ORF cDNA Clones and Custom Clones » Species Summary » Homo sapiens » WNK3 cDNA ORF clone

WNK3 cDNA ORF clone, Homo sapiens(human)

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Summary

Gene Symbol	WNK3
Entrez Gene ID	65267
Full Name	WNK lysine deficient protein kinase 3
Synonyms	PRKWNK3
General protein information	Preferred Names WNK lysine deficient protein kinase 3 Names serine/threonine-protein kinase WNK3 protein kinase with no lysine 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.22
Summary	This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010].
Disorder MIM:	300358

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017029742.1	XP_016885231.1	serine/threonine-protein kinase WNK3 isoform X1
XM_017029743.1	XP_016885232.1	serine/threonine-protein kinase WNK3 isoform X1
XM_017029744.1	XP_016885233.1	serine/threonine-protein kinase WNK3 isoform X1
XM_006724593.3	XP_006724656.1	serine/threonine-protein kinase WNK3 isoform X4
NM_001002838.4	NP_001002838.1	serine/threonine-protein kinase WNK3 isoform 2
NM_020922.4	NP_065973.2	serine/threonine-protein kinase WNK3 isoform 1
XM_017029745.1	XP_016885234.1	serine/threonine-protein kinase WNK3 isoform X2
XM_017029741.1	XP_016885230.1	serine/threonine-protein kinase WNK3 isoform X1
NM_001002838.3	NP_001002838.1	serine/threonine-protein kinase WNK3 isoform 2
XM_011530802.2	XP_011529104.1	serine/threonine-protein kinase WNK3 isoform X3

Homologies

Pan troglodytes (chimpanzee)	WNK3	XP_521078.2
Homo sapiens (human)	WNK3	NP_065973.2
Canis lupus familiaris (dog)	WNK3	XP_864627.1
Mus musculus (house mouse)	Wnk3	NP_001258607.1
Bos taurus (cattle)	WNK3	NP_001243516.1
Rattus norvegicus (Norway rat)	Wnk3	NP_001157079.1
	WNK3	XP_001089672.1

Bibliography

Related articles in PubMed

WNK3 interacts with NCC.
Wang D, Pan S, Xu Y, Ye X, Ren X, Zeng Q
Science China. Life sciences59(11)1189-1191(2016 Nov)

WNK3 Kinase Enhances the Sodium Chloride Cotransporter Expression via an ERK 1/2 Signaling Pathway.
Wang D, Zhang Y, Han J, Pan S, Xu N, Feng X, Zhuang Z, Caroti C, Zhuang J, Hoover RS, Gu D, Zeng Q, Cai H
Nephron133(4)287-95(2016)

Inhibition of WNK3 Kinase Signaling Reduces Brain Damage and Accelerates Neurological Recovery After Stroke.
Begum G, Yuan H, Kahle KT, Li L, Wang S, Shi Y, Shmukler BE, Yang SS, Lin SH, Alper SL, Sun D
Stroke46(7)1956-1965(2015 Jul)

WNK3 abrogates the NEDD4-2-mediated inhibition of the renal Na+-Cl- cotransporter.
Lagnaz D, Arroyo JP, Ch?vez-Canales M, V?zquez N, Rizzo F, Spirl? A, Debonneville A, Staub O, Gamba G
American journal of physiology. Renal physiology307(3)F275-86(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that WNK lysine deficient protein kinase 3 protein (WNK3) interacts with NCC and increases the Na-Cl cotransporter (NCC) expression on the cell membrane and in cytoplasm together.
Title: WNK3 interacts with NCC.

WNK3 enhances NCC protein expression by increasing NCC synthesis via an ERK 1/2-dependent signaling pathway
Title: WNK3 Kinase Enhances the Sodium Chloride Cotransporter Expression via an ERK 1/2 Signaling Pathway.

WNK3 knockout mice exhibited significantly decreased infarct volume in a transgenic model of stroke and brain damage.
Title: Inhibition of WNK3 Kinase Signaling Reduces Brain Damage and Accelerates Neurological Recovery After Stroke.

There may be a novel role for WNK3 on the renal Na+-Cl-cotransporter (NCC) expression at the plasma membrane, an effect apparently independent of the SPAK kinase and the aldosterone-SGK1 pathway.
Title: WNK3 abrogates the NEDD4-2-mediated inhibition of the renal Na+-Cl- cotransporter.

LINGO-1 potentiates neuronal apoptosis, likely by inhibiting WNK3 kinase activity.
Title: LINGO-1 receptor promotes neuronal apoptosis by inhibiting WNK3 kinase activity.

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The following WNK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu99431	XM_017029745.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OHu21239	NM_001002838.4 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu21773	NM_020922.4 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu21239	XM_006724593.3 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X7, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu21773	XM_017029743.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu21773	XM_017029744.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu21773	XM_017029741.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu21773	XM_017029742.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
OHu71645	XM_011530802.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu21239	NM_001002838.3	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WNK3 ( XM_017029745.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885234.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X5, mRNA.

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CloneID	OHu99431
Clone ID Related Accession (Same CDS sequence)	XM_017029745.1
Accession Version	XM_017029745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG ATCCACTGTG TGTGGAGAGT 
AATGCAGCAT CATGCCAACA GTCTCCAGCC AGTAAAAAAG GGATGTTCAC AGATGACTTA 
CACAAGCTGG TGGATGACTG GACAAAGGAA GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT 
TTAAACCAAC TTAAACAAAG TCAACACAAA CTAGAGACAG AAAACTGGAA TAAAGTATCT 
GAAAATACTC CGTCTACTAT GGGCTACACA TCAACATGGA TTTCTTCTCT GTCCCAAATC 
CGTGGAGCTG TCCCAACTTC CTTGCCACAA GGACTCTCAC TCCCTTCATT TCCTGGGCCA 
TTATCATCAT ATGGAATGCC TCACGTTTGT CAGTATAATG CTGTGGCGGG GGCGGGGTAT 
CCAGTACAGT GGGTAGGAAT TTCAGGAACA ACACAACAAT CTGTAGTAAT TCCCGCCCAA 
TCTGGGGGAC CATTCCAGCC AGGGATGAAT ATGCAGGCAT TTCCAACTTC ATCAGTGCAG 
AATCCTGCCA CAATCCCTCC TGGTCCTAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885234.1
CDS	378..5750
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATDPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_017029745.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029745.1

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG ATCCACTGTG TGTGGAGAGT 
AATGCAGCAT CATGCCAACA GTCTCCAGCC AGTAAAAAAG GGATGTTCAC AGATGACTTA 
CACAAGCTGG TGGATGACTG GACAAAGGAA GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT 
TTAAACCAAC TTAAACAAAG TCAACACAAA CTAGAGACAG AAAACTGGAA TAAAGTATCT 
GAAAATACTC CGTCTACTAT GGGCTACACA TCAACATGGA TTTCTTCTCT GTCCCAAATC 
CGTGGAGCTG TCCCAACTTC CTTGCCACAA GGACTCTCAC TCCCTTCATT TCCTGGGCCA 
TTATCATCAT ATGGAATGCC TCACGTTTGT CAGTATAATG CTGTGGCGGG GGCGGGGTAT 
CCAGTACAGT GGGTAGGAAT TTCAGGAACA ACACAACAAT CTGTAGTAAT TCCCGCCCAA 
TCTGGGGGAC CATTCCAGCC AGGGATGAAT ATGCAGGCAT TTCCAACTTC ATCAGTGCAG 
AATCCTGCCA CAATCCCTCC TGGTCCTAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( NM_001002838.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001002838.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 2, mRNA.

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CloneID	OHu21239
Clone ID Related Accession (Same CDS sequence)	XM_006724593.3 , NM_001002838.4 , NM_001002838.3
Accession Version	NM_001002838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591766.20, AY352048.1, AJ409088.2, Z84469.1 and R44257.1. On Jun 2, 2019 this sequence version replaced NM_001002838.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ409088.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGA TCCACTGTGT GTGGAGAGTA ATGCAGCATC ATGCCAACAG 
TCTCCAGCCA GTAAAAAAGG GATGTTCACA GATGACTTAC ACAAGCTGGT GGATGACTGG 
ACAAAGGAAG CAGTAGGAAA TTCTCTTATT AAGCCAAGTT TAAACCAACT TAAACAAAGT 
CAACACAAAC TAGAGACAGA AAACTGGAAT AAAGTATCTG AAAATACTCC GTCTACTATG 
GGCTACACAT CAACATGGAT TTCTTCTCTG TCCCAAATCC GTGGAGCTGT CCCAACTTCC 
TTGCCACAAG GACTCTCACT CCCTTCATTT CCTGGGCCAT TATCATCATA TGGAATGCCT 
CACGTTTGTC AGTATAATGC TGTGGCGGGG GCGGGGTATC CAGTACAGTG GGTAGGAATT 
TCAGGAACAA CACAACAATC TGTAGTAATT CCCGCCCAAT CTGGGGGACC ATTCCAGCCA 
GGGATGAATA TGCAGGCATT TCCAACTTCA TCAGTGCAGA ATCCTGCCAC AATCCCTCCT 
GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002838.1
CDS	481..5712
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATDPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	NM_001002838.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002838.4

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGA TCCACTGTGT GTGGAGAGTA ATGCAGCATC ATGCCAACAG 
TCTCCAGCCA GTAAAAAAGG GATGTTCACA GATGACTTAC ACAAGCTGGT GGATGACTGG 
ACAAAGGAAG CAGTAGGAAA TTCTCTTATT AAGCCAAGTT TAAACCAACT TAAACAAAGT 
CAACACAAAC TAGAGACAGA AAACTGGAAT AAAGTATCTG AAAATACTCC GTCTACTATG 
GGCTACACAT CAACATGGAT TTCTTCTCTG TCCCAAATCC GTGGAGCTGT CCCAACTTCC 
TTGCCACAAG GACTCTCACT CCCTTCATTT CCTGGGCCAT TATCATCATA TGGAATGCCT 
CACGTTTGTC AGTATAATGC TGTGGCGGGG GCGGGGTATC CAGTACAGTG GGTAGGAATT 
TCAGGAACAA CACAACAATC TGTAGTAATT CCCGCCCAAT CTGGGGGACC ATTCCAGCCA 
GGGATGAATA TGCAGGCATT TCCAACTTCA TCAGTGCAGA ATCCTGCCAC AATCCCTCCT 
GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( NM_001002838.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001002838.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 2, mRNA.

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CloneID	OHu21239
Clone ID Related Accession (Same CDS sequence)	XM_006724593.3 , NM_001002838.4 , NM_001002838.3
Accession Version	NM_001002838.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY352048.1, AJ409088.2, Z84469.1 and R44257.1. On May 15, 2010 this sequence version replaced NM_001002838.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ409088.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGA TCCACTGTGT GTGGAGAGTA ATGCAGCATC ATGCCAACAG 
TCTCCAGCCA GTAAAAAAGG GATGTTCACA GATGACTTAC ACAAGCTGGT GGATGACTGG 
ACAAAGGAAG CAGTAGGAAA TTCTCTTATT AAGCCAAGTT TAAACCAACT TAAACAAAGT 
CAACACAAAC TAGAGACAGA AAACTGGAAT AAAGTATCTG AAAATACTCC GTCTACTATG 
GGCTACACAT CAACATGGAT TTCTTCTCTG TCCCAAATCC GTGGAGCTGT CCCAACTTCC 
TTGCCACAAG GACTCTCACT CCCTTCATTT CCTGGGCCAT TATCATCATA TGGAATGCCT 
CACGTTTGTC AGTATAATGC TGTGGCGGGG GCGGGGTATC CAGTACAGTG GGTAGGAATT 
TCAGGAACAA CACAACAATC TGTAGTAATT CCCGCCCAAT CTGGGGGACC ATTCCAGCCA 
GGGATGAATA TGCAGGCATT TCCAACTTCA TCAGTGCAGA ATCCTGCCAC AATCCCTCCT 
GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002838.1
CDS	440..5671
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATDPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	NM_001002838.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002838.3

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGA TCCACTGTGT GTGGAGAGTA ATGCAGCATC ATGCCAACAG 
TCTCCAGCCA GTAAAAAAGG GATGTTCACA GATGACTTAC ACAAGCTGGT GGATGACTGG 
ACAAAGGAAG CAGTAGGAAA TTCTCTTATT AAGCCAAGTT TAAACCAACT TAAACAAAGT 
CAACACAAAC TAGAGACAGA AAACTGGAAT AAAGTATCTG AAAATACTCC GTCTACTATG 
GGCTACACAT CAACATGGAT TTCTTCTCTG TCCCAAATCC GTGGAGCTGT CCCAACTTCC 
TTGCCACAAG GACTCTCACT CCCTTCATTT CCTGGGCCAT TATCATCATA TGGAATGCCT 
CACGTTTGTC AGTATAATGC TGTGGCGGGG GCGGGGTATC CAGTACAGTG GGTAGGAATT 
TCAGGAACAA CACAACAATC TGTAGTAATT CCCGCCCAAT CTGGGGGACC ATTCCAGCCA 
GGGATGAATA TGCAGGCATT TCCAACTTCA TCAGTGCAGA ATCCTGCCAC AATCCCTCCT 
GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( NM_020922.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065973.2 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 1, mRNA.

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CloneID	OHu21773
Clone ID Related Accession (Same CDS sequence)	XM_017029742.1 , XM_017029743.1 , XM_017029744.1 , NM_020922.4 , XM_017029741.1
Accession Version	NM_020922.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-13
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY352048.1, Z84469.1 and R44257.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2010 this sequence version replaced NM_020922.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY352048.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065973.2
CDS	440..5842
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	NM_020922.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020922.4

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_006724593.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724656.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X7, mRNA.

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CloneID	OHu21239
Clone ID Related Accession (Same CDS sequence)	XM_006724593.3 , NM_001002838.4 , NM_001002838.3
Accession Version	XM_006724593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGA TCCACTGTGT GTGGAGAGTA ATGCAGCATC ATGCCAACAG 
TCTCCAGCCA GTAAAAAAGG GATGTTCACA GATGACTTAC ACAAGCTGGT GGATGACTGG 
ACAAAGGAAG CAGTAGGAAA TTCTCTTATT AAGCCAAGTT TAAACCAACT TAAACAAAGT 
CAACACAAAC TAGAGACAGA AAACTGGAAT AAAGTATCTG AAAATACTCC GTCTACTATG 
GGCTACACAT CAACATGGAT TTCTTCTCTG TCCCAAATCC GTGGAGCTGT CCCAACTTCC 
TTGCCACAAG GACTCTCACT CCCTTCATTT CCTGGGCCAT TATCATCATA TGGAATGCCT 
CACGTTTGTC AGTATAATGC TGTGGCGGGG GCGGGGTATC CAGTACAGTG GGTAGGAATT 
TCAGGAACAA CACAACAATC TGTAGTAATT CCCGCCCAAT CTGGGGGACC ATTCCAGCCA 
GGGATGAATA TGCAGGCATT TCCAACTTCA TCAGTGCAGA ATCCTGCCAC AATCCCTCCT 
GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724656.1
CDS	378..5609
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATDPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_006724593.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724593.3

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGA TCCACTGTGT GTGGAGAGTA ATGCAGCATC ATGCCAACAG 
TCTCCAGCCA GTAAAAAAGG GATGTTCACA GATGACTTAC ACAAGCTGGT GGATGACTGG 
ACAAAGGAAG CAGTAGGAAA TTCTCTTATT AAGCCAAGTT TAAACCAACT TAAACAAAGT 
CAACACAAAC TAGAGACAGA AAACTGGAAT AAAGTATCTG AAAATACTCC GTCTACTATG 
GGCTACACAT CAACATGGAT TTCTTCTCTG TCCCAAATCC GTGGAGCTGT CCCAACTTCC 
TTGCCACAAG GACTCTCACT CCCTTCATTT CCTGGGCCAT TATCATCATA TGGAATGCCT 
CACGTTTGTC AGTATAATGC TGTGGCGGGG GCGGGGTATC CAGTACAGTG GGTAGGAATT 
TCAGGAACAA CACAACAATC TGTAGTAATT CCCGCCCAAT CTGGGGGACC ATTCCAGCCA 
GGGATGAATA TGCAGGCATT TCCAACTTCA TCAGTGCAGA ATCCTGCCAC AATCCCTCCT 
GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_017029742.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885231.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X2, mRNA.

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CloneID	OHu21773
Clone ID Related Accession (Same CDS sequence)	XM_017029742.1 , XM_017029743.1 , XM_017029744.1 , NM_020922.4 , XM_017029741.1
Accession Version	XM_017029742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885231.1
CDS	456..5858
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_017029742.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029742.1

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_017029743.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885232.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X3, mRNA.

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CloneID	OHu21773
Clone ID Related Accession (Same CDS sequence)	XM_017029742.1 , XM_017029743.1 , XM_017029744.1 , NM_020922.4 , XM_017029741.1
Accession Version	XM_017029743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885232.1
CDS	371..5773
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_017029743.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029743.1

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_017029744.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885233.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.

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CloneID	OHu21773
Clone ID Related Accession (Same CDS sequence)	XM_017029742.1 , XM_017029743.1 , XM_017029744.1 , NM_020922.4 , XM_017029741.1
Accession Version	XM_017029744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885233.1
CDS	273..5675
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_017029744.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029744.1

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_017029741.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885230.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.

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CloneID	OHu21773
Clone ID Related Accession (Same CDS sequence)	XM_017029742.1 , XM_017029743.1 , XM_017029744.1 , NM_020922.4 , XM_017029741.1
Accession Version	XM_017029741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885230.1
CDS	379..5781
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_017029741.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029741.1

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGGTGGATAT TTTGGCCTAA GCTTTACTTG TCCTAGTCTC 
AAAAATCCTA TTAGCAAGAA ATCCTGGACT CGCAAATTAA AAAGCTGGGC ATACAGGCTA 
CGGCAGTCAA CCAGCTTTTT CAAGAGATCA AAAGTCCGTC AAGTGGAAAC AGAAGAGATG 
AGATCAGCAA TTGCTCCTGA TCCCATTCCT CTGACACGGG AGTCCACAGC TGATACTAGG 
GCTTTGAATA GATGTAAAGC GATGAGTGGA TCATTTCAGC GGGGTCGGTT CCAGGTGATT 
ACAATTCCTC AGCAGCAGTC AGCAAAAATG ACATCTTTTG GAATAGAACA CATATCAGTG 
TTCAGTGAGA CAAACCATTC TAGTGAAGAA GCCTTTATTA AAACAGCAAA GTCTCAGTTG 
GTAGAAATAG AACCTGCCAC ACAAAATCCA AAAACTTCGT TTTCTTATGA GAAGTTACAA 
GCTCTTCAGG AAACCTGTAA AGAAAATAAA GGAGTTCCCA AACAAGGTGA CAACTTCTTA 
TCTTTCAGCG CAGCTTGTGA GACTGATGTA TCTTCAGTGA CCCCAGAAAA GGAATTTGAA 
GAAACTTCAG CCACAGGAAG TAGCATGCAG TCTGGATCTG AACTGTTGCT TAAAGAGAGA 
GAGATATTGA CTGCTGGGAA ACAGCCTAGC TCTGATAGTG AATTTTCAGC CAGTCTTGCT 
GGCAGTGGAA AGTCAGTGGC AAAGACTGGT CCAGAGAGTA ATCAGTGCTT ACCACACCAC 
GAAGAACAAG CTTATGCTCA AACACAGAGT TCACTCTTCT ATTCGCCATC TTCCCCAATG 
AGCAGTGATG ATGAATCAGA AATAGAGGAT GAGGACTTGA AGGTGGAGCT TCAAAGATTA 
CGAGAAAAAC ACATTCAGGA GGTGGTAAAT CTTCAAACCC AGCAGAATAA GGAGCTGCAG 
GAGCTCTATG AACGCCTTCG GTCAATTAAA GATAGCAAAA CCCAATCTAC TGAGATTCCT 
TTGCCACCTG CATCACCACG TCGACCAAGA TCTTTCAAAA GCAAACTTCG AAGCCGCCCC 
CAGTCCTTGA CACATGTGGA CAATGGCATA GTTGCTACAG GGAAAAGCTG CCTTATAAAT 
GAGTTGGAAA ATCCACTGTG TGTGGAGAGT AATGCAGCAT CATGCCAACA GTCTCCAGCC 
AGTAAAAAAG GGATGTTCAC AGATGACTTA CACAAGCTGG TGGATGACTG GACAAAGGAA 
GCAGTAGGAA ATTCTCTTAT TAAGCCAAGT TTAAACCAAC TTAAACAAAG TCAACACAAA 
CTAGAGACAG AAAACTGGAA TAAAGTATCT GAAAATACTC CGTCTACTAT GGGCTACACA 
TCAACATGGA TTTCTTCTCT GTCCCAAATC CGTGGAGCTG TCCCAACTTC CTTGCCACAA 
GGACTCTCAC TCCCTTCATT TCCTGGGCCA TTATCATCAT ATGGAATGCC TCACGTTTGT 
CAGTATAATG CTGTGGCGGG GGCGGGGTAT CCAGTACAGT GGGTAGGAAT TTCAGGAACA 
ACACAACAAT CTGTAGTAAT TCCCGCCCAA TCTGGGGGAC CATTCCAGCC AGGGATGAAT 
ATGCAGGCAT TTCCAACTTC ATCAGTGCAG AATCCTGCCA CAATCCCTCC TGGTCCTAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_011530802.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529104.1 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X6, mRNA.

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CloneID	OHu71645
Clone ID Related Accession (Same CDS sequence)	XM_011530802.2
Accession Version	XM_011530802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGG GAAAAGCTGC CTTATAAATG AGTTGGAAAA TCCACTGTGT 
GTGGAGAGTA ATGCAGCATC ATGCCAACAG TCTCCAGCCA GTAAAAAAGG GATGTTCACA 
GATGACTTAC ACAAGCTGGT GGATGACTGG ACAAAGGAAG CAGTAGGAAA TTCTCTTATT 
AAGCCAAGTT TAAACCAACT TAAACAAAGT CAACACAAAC TAGAGACAGA AAACTGGAAT 
AAAGTATCTG AAAATACTCC GTCTACTATG GGCTACACAT CAACATGGAT TTCTTCTCTG 
TCCCAAATCC GTGGAGCTGT CCCAACTTCC TTGCCACAAG GACTCTCACT CCCTTCATTT 
CCTGGGCCAT TATCATCATA TGGAATGCCT CACGTTTGTC AGTATAATGC TGTGGCGGGG 
GCGGGGTATC CAGTACAGTG GGTAGGAATT TCAGGAACAA CACAACAATC TGTAGTAATT 
CCCGCCCAAT CTGGGGGACC ATTCCAGCCA GGGATGAATA TGCAGGCATT TCCAACTTCA 
TCAGTGCAGA ATCCTGCCAC AATCCCTCCT GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529104.1
CDS	378..5639
Translation	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Target ORF information:

RefSeq Version	XM_011530802.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530802.2

ORF Insert Sequence:

ATGGCCACTG ATTCAGGGGA TCCAGCCAGC ACAGAAGATT CTGAGAAACC TGATGGAATT 
TCATTTGAAA ACAGAGTTCC CCAGGTCGCT GCAACTTTGA CAGTAGAAGC TAGACTAAAG 
GAGAAAAACA GTACCTTCTC TGCTTCTGGG GAAACTGTAG AAAGGAAGAG ATTTTTCCGA 
AAGAGTGTTG AAATGACGGA AGATGACAAA GTTGCCGAAT CATCCCCCAA AGATGAGAGA 
ATTAAGGCTG CAATGAATAT TCCAAGAGTA GATAAGCTTC CTTCAAATGT GTTGAGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGTCAACCT CAGAAATCTC AAAAGATTGT 
TTCAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGATTCCT GAAATTTGAC ATAGAACTAG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GACTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGAAAGTTAA CCAAAGCTGA GCAGCAAAGA TTCAAGGAAG AAGCAGAGAT GTTGAAGGGT 
CTCCAGCACC CCAATATAGT TCGATTTTAT GATTCCTGGG AATCTATATT AAAAGGAAAG 
AAATGTATTG TATTAGTGAC TGAACTAATG ACATCTGGGA CCTTAAAGAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGCTGGT GCAGGCAAAT TTTAAAGGGG 
TTGCAGTTCT TGCACACTAG GACTCCTCCT ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGATT AGCCACCTTA 
ATGCGTACCT CATTTGCTAA GAGTGTCATT GGAACTCCTG AGTTTATGGC TCCAGAGATG 
TATGAAGAAC ACTATGATGA ATCCGTAGAT GTTTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCCACAT CGGAGTATCC TTATTCTGAG TGTCAGAATG CAGCTCAAAT ATACCGGAAA 
GTAACTAGTG GCATAAAACC AGCCAGCTTC AATAAAGTCA CTGATCCTGA AGTCAAAGAA 
ATCATTGAAG GATGTATTCG TCAAAACAAA TCTGAAAGGT TGTCTATCAG GGACCTATTA 
AACCATGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA AGAAGATGAT 
TGCTCAAATT CATCCCTTGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTT AGTTTCAACT TAGAAACAGA TACACCTGAG 
GAAGTAGCAT ATGAAATGGT CAAGTCTGGG TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAATCCA TTAGAGACCG GGTGACGCCA ATAAAGAAGA CAAGAGAGAA GAAGCCTGCT 
GGCTGTTTGG AAGAACGCAG GGATTCTCAG TGCAAGTCTA TGGGGAATGT ATTCCCTCAG 
CCCCAGAATA CAACTTTACC CCTTGCTCCC GCTCAGCAAA CTGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAACATGT TAGACAACAG CTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG CATCAGTTAT ACATTCAGAT 
ACTTCAAGTC AGCCCAGTGT AGCCTATTCC TCAAATCAAA CGATGGGCTC TCAAATGGTT 
TCTAATATCC CGCAGGCTGA AGTAAATGTT CCAGGGCAAA TTTATTCATC TCAGCAACTA 
GTAGGACATT ACCAGCAAGT TTCAGGGTTA CAGAAGCATT CAAAGCTGAC TCAGCCGCAG 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTACCTG TACATGTCCT TGGACCGACA 
GTTGTTTCAC AACCCCAGGT TTCCCCATTA ACTGTTCAGA AGGTCCCACA GATAAAGCCT 
GTATCCCAAC CAGTTGGAGC TGAACAACAA GCAGCTCTTC TAAAACCAGA TTTAGTTCGA 
AGCTTGAATC AAGATGTGGC AACTACGAAG GAAAACGTCA GTAGCCCTGA TAACCCAAGT 
GGAAATGGCA AACAGGATCG GATCAAACAG AGAAGAGCTT CCTGTCCCCG ACCAGAGAAG 
GGGACTAAAT TTCAGCTTAC CGTCCTTCAG GTATCAACCT CTGGAGATAA CATGGTAGAG 
TGTCAGCTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGT TGATGGTGAT 
GCGCCAGAGG ATATTGCAGA CTATATGGTT GAAGATAACT TTGTGCTGGA AAGTGAGAAA 
GAAAAATTTG TAGAAGAATT GAGAGCTATT GTAGGTCAAG CCCAGGAGAT CCTTCATGTC 
CACTTTGCCA CAGAAAGAGC CACTGGAGTT GATTCTATTA CTGTGGACTC CAACAGTAGC 
CAGACAGGGT CATCTGAACA AGTACAGATA AATTCTACAT CTACTCAAAC CAGCAATGAA 
TCTGCTCCTC AGTCATCCCC AGTTGGTCGG TGGCGATTCT GTATCAATCA GACGATAAGA 
AACCGTGAGA CTCAGTCTCC TCCTTCTCTT CAGCATTCCA TGTCTGCGGT TCCTGGCCGA 
CATCCACTTC CTAGTCCAAA AAACACAAGT AATAAGGAAA TATCACGGGA CACATTGCTC 
ACTATAGAAA ATAATCCATG CCACCGTGCA CTTTTCACCT CCAAATCAGA ACACAAGGAT 
GTGGTTGATG GTAAGATTTC TGAATGTGCT AGTGTAGAAA CCAAGCAGCC AGCTATACTT 
TATCAAGTGG AAGATAACAG GCAGATAATG GCACCAGTTA CTAATAGTTC CAGTTACTCT 
ACTACTTCAG TTCGTGCAGT TCCAGCTGAA TGTGAGGGAC TCACCAAACA AGCAAGCATA 
TTCATACCTG TGTATCCATG TCACCAAACT GCCAGTCAGG CTGATGCACT TATGTCCCAT 
CCTGGCGAAT CAACTCAGAC TTCTGGTAAC TCTCTTACAA CTCTGGCATT TGATCAAAAG 
CCTCAAACCT TATCAGTACA GCAGCCAGCT ATGGATGCAG AGTTTATTTC TCAAGAAGGA 
GAAACTACAG TGAACACTGA AGCAAGTTCT CCTAAGACAG TCATTCCCAC TCAGACCCCT 
GGCCTTGAAC CAACTACCCT TCAACCCACT ACTGTCCTGG AATCAGATGG AGAAAGACCT 
CCAAAACTGG AGTTTGCAGA CAACCGAATT AAAACTCTGG ATGAAAAATT AAGAAACTTG 
CTCTATCAGG AGCACAGCAT CTCTAGCATC TATCCCGAGA GTCAGAAGGA TACCCAAAGC 
ATAGACTCTC CATTTTCTTC CTCTGCTGAA GATACCCTCT CCTGTCCAGT GACAGAAGTC 
ATAGCCATCA GTCACTGTGG AATTAAAGAT AGCCCTGTAC AATCCCCTAA TTTCCAACAG 
ACAGGCTCTA AGCTTCTGTC CAATGTGGCT GCAAGTCAGC CTGCTAATAT ATCAGTGTTC 
AAAAGGGACC TGAATGTGAT AACTTCTGTA CCCAGCGAAT TGTGTTTACA TGAGATGTCC 
TCAGATGCTT CACTTCCAGG GGATCCAGAG GCCTATCCTG CTGCTGTGTC AAGCGGTGGA 
GCCATTCATC TGCAGACAGG AGTGGAAACA GAAGAGATGA GATCAGCAAT TGCTCCTGAT 
CCCATTCCTC TGACACGGGA GTCCACAGCT GATACTAGGG CTTTGAATAG ATGTAAAGCG 
ATGAGTGGAT CATTTCAGCG GGGTCGGTTC CAGGTGATTA CAATTCCTCA GCAGCAGTCA 
GCAAAAATGA CATCTTTTGG AATAGAACAC ATATCAGTGT TCAGTGAGAC AAACCATTCT 
AGTGAAGAAG CCTTTATTAA AACAGCAAAG TCTCAGTTGG TAGAAATAGA ACCTGCCACA 
CAAAATCCAA AAACTTCGTT TTCTTATGAG AAGTTACAAG CTCTTCAGGA AACCTGTAAA 
GAAAATAAAG GAGTTCCCAA ACAAGGTGAC AACTTCTTAT CTTTCAGCGC AGCTTGTGAG 
ACTGATGTAT CTTCAGTGAC CCCAGAAAAG GAATTTGAAG AAACTTCAGC CACAGGAAGT 
AGCATGCAGT CTGGATCTGA ACTGTTGCTT AAAGAGAGAG AGATATTGAC TGCTGGGAAA 
CAGCCTAGCT CTGATAGTGA ATTTTCAGCC AGTCTTGCTG GCAGTGGAAA GTCAGTGGCA 
AAGACTGGTC CAGAGAGTAA TCAGTGCTTA CCACACCACG AAGAACAAGC TTATGCTCAA 
ACACAGAGTT CACTCTTCTA TTCGCCATCT TCCCCAATGA GCAGTGATGA TGAATCAGAA 
ATAGAGGATG AGGACTTGAA GGTGGAGCTT CAAAGATTAC GAGAAAAACA CATTCAGGAG 
GTGGTAAATC TTCAAACCCA GCAGAATAAG GAGCTGCAGG AGCTCTATGA ACGCCTTCGG 
TCAATTAAAG ATAGCAAAAC CCAATCTACT GAGATTCCTT TGCCACCTGC ATCACCACGT 
CGACCAAGAT CTTTCAAAAG CAAACTTCGA AGCCGCCCCC AGTCCTTGAC ACATGTGGAC 
AATGGCATAG TTGCTACAGG GAAAAGCTGC CTTATAAATG AGTTGGAAAA TCCACTGTGT 
GTGGAGAGTA ATGCAGCATC ATGCCAACAG TCTCCAGCCA GTAAAAAAGG GATGTTCACA 
GATGACTTAC ACAAGCTGGT GGATGACTGG ACAAAGGAAG CAGTAGGAAA TTCTCTTATT 
AAGCCAAGTT TAAACCAACT TAAACAAAGT CAACACAAAC TAGAGACAGA AAACTGGAAT 
AAAGTATCTG AAAATACTCC GTCTACTATG GGCTACACAT CAACATGGAT TTCTTCTCTG 
TCCCAAATCC GTGGAGCTGT CCCAACTTCC TTGCCACAAG GACTCTCACT CCCTTCATTT 
CCTGGGCCAT TATCATCATA TGGAATGCCT CACGTTTGTC AGTATAATGC TGTGGCGGGG 
GCGGGGTATC CAGTACAGTG GGTAGGAATT TCAGGAACAA CACAACAATC TGTAGTAATT 
CCCGCCCAAT CTGGGGGACC ATTCCAGCCA GGGATGAATA TGCAGGCATT TCCAACTTCA 
TCAGTGCAGA ATCCTGCCAC AATCCCTCCT GGTCCTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WNK3 interacts with NCC.
Science China. Life sciences59(11)1189-1191(2016 Nov)
Wang D,Pan S,Xu Y,Ye X,Ren X,Zeng Q

WNK3 Kinase Enhances the Sodium Chloride Cotransporter Expression via an ERK 1/2 Signaling Pathway.
Nephron133(4)287-95(2016)
Wang D,Zhang Y,Han J,Pan S,Xu N,Feng X,Zhuang Z,Caroti C,Zhuang J,Hoover RS,Gu D,Zeng Q,Cai H

Inhibition of WNK3 Kinase Signaling Reduces Brain Damage and Accelerates Neurological Recovery After Stroke.
Stroke46(7)1956-1965(2015 Jul)
Begum G,Yuan H,Kahle KT,Li L,Wang S,Shi Y,Shmukler BE,Yang SS,Lin SH,Alper SL,Sun D

WNK3 abrogates the NEDD4-2-mediated inhibition of the renal Na+-Cl- cotransporter.
American journal of physiology. Renal physiology307(3)F275-86(2014 Aug)
Lagnaz D,Arroyo JP,Ch?vez-Canales M,V?zquez N,Rizzo F,Spirl? A,Debonneville A,Staub O,Gamba G

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