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SLC12A3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SLC12A3
Entrez Gene ID 6559
Full Name solute carrier family 12 (sodium/chloride transporter), member 3
Synonyms NCC, NCCT, TSC
General protein information
Preferred Names
solute carrier family 12 member 3
Names
solute carrier family 12 member 3
Na-Cl symporter
Na-Cl cotransporter
thiazide-sensitive Na-Cl cotransporter
thiazide-sensitive sodium-chloride cotransporter
NaCl electroneutral thiazide-sensitive cotransporter
solute carrier family 12 (sodium/chloride transporters), member 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q13

Summary This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600968

Disorder Html: Gitelman syndrome, 263800 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005256119 XP_005256176 solute carrier family 12 member 3 isoform X1
NM_000339 NP_000330 solute carrier family 12 member 3 isoform 1
NM_001126107 NP_001119579 solute carrier family 12 member 3 isoform 2
NM_001126108 NP_001119580 solute carrier family 12 member 3 isoform 3



Homo sapiens (human) SLC12A3 NP_000330.2
Pan troglodytes (chimpanzee) SLC12A3 XP_510983.2
Macaca mulatta (Rhesus monkey) SLC12A3 XP_001093467.2
Canis lupus familiaris (dog) SLC12A3 XP_005617594.1
Bos taurus (cattle) SLC12A3 NP_001193107.1
Mus musculus (house mouse) Slc12a3 NP_062288.2
Rattus norvegicus (Norway rat) Slc12a3 NP_062218.3
Gallus gallus (chicken) SLC12A3 XP_414059.4
Danio rerio (zebrafish) slc12a3 NP_001038545.1
Xenopus (Silurana) tropicalis (western clawed frog) slc12a3 XP_002937217.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu49480 XM_005256119 PREDICTED: Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu26218 NM_000339 Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $727.30
$1039.00
OHu26776 NM_001126107 Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $727.30
$1039.00
OHu26429 NM_001126108 Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $720.30
$1029.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu49480
Clone ID Related Accession (Same CDS sequence) XM_005256119
Accession Version XM_005256119.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC
ATCATTGCCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG
GAAGCCAGCG CCAGAGTGGA CCCCAAGGCC CTGGTGAAGG AGGAGCAGGC CACCACCATC
TTCCAGTCGG AGCAGGGCAA GAAGACCATA GACATCTACT GGCTCTTTGA CGATGGAGGC
CTCACCCTCC TCATTCCCTA TCTCCTTGGC CGCAAGAGGA GGTGGAGCAA ATGCAAGATC
CGTGTGTTCG TAGGCGGCCA GATTAACAGG ATGGACCAGG AGAGAAAGGC GATCATTTCT
CTGCTGAGCA AGTTCCGACT GGGATTCCAT GAAGTCCACA TCCTCCCTGA CATCAACCAG
AACCCTCGGG CTGAGCACAC CAAGAGGTTT GAGGACATGA TTGCACCCTT CCGTCTGAAT
GATGGCTTCA AGGATGAGGC CACTGTCAAC GAGATGCGGC GGGACTGCCC CTGGAAGATC
TCAGATGAGG AGATTACGAA GAACAGAGTC AAGTCCCTTC GGCAGGTGAG GCTGAATGAG
ATTGTGCTGG ATTACTCCCG AGACGCTGCT CTCATCGTCA TCACTTTGCC CATAGGGAGG
AAGGGGAAGT GCCCCAGCTC GCTGTACATG GCCTGGCTGG AGACCCTGTC CCAGGACCTC
AGACCTCCAG TCATCCTGAT CCGAGGAAAC CAGGAAAACG TGCTCACCTT TTACTGCCAG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256176.1
CDS30..3092
Misc Feature(1)159..383(+)
Misc Feature(2)204..3086(+)
Translation

Target ORF information:

RefSeq Version XM_005256119
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005256119

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC
ATCATTGCCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG
GAAGCCAGCG CCAGAGTGGA CCCCAAGGCC CTGGTGAAGG AGGAGCAGGC CACCACCATC
TTCCAGTCGG AGCAGGGCAA GAAGACCATA GACATCTACT GGCTCTTTGA CGATGGAGGC
CTCACCCTCC TCATTCCCTA TCTCCTTGGC CGCAAGAGGA GGTGGAGCAA ATGCAAGATC
CGTGTGTTCG TAGGCGGCCA GATTAACAGG ATGGACCAGG AGAGAAAGGC GATCATTTCT
CTGCTGAGCA AGTTCCGACT GGGATTCCAT GAAGTCCACA TCCTCCCTGA CATCAACCAG
AACCCTCGGG CTGAGCACAC CAAGAGGTTT GAGGACATGA TTGCACCCTT CCGTCTGAAT
GATGGCTTCA AGGATGAGGC CACTGTCAAC GAGATGCGGC GGGACTGCCC CTGGAAGATC
TCAGATGAGG AGATTACGAA GAACAGAGTC AAGTCCCTTC GGCAGGTGAG GCTGAATGAG
ATTGTGCTGG ATTACTCCCG AGACGCTGCT CTCATCGTCA TCACTTTGCC CATAGGGAGG
AAGGGGAAGT GCCCCAGCTC GCTGTACATG GCCTGGCTGG AGACCCTGTC CCAGGACCTC
AGACCTCCAG TCATCCTGAT CCGAGGAAAC CAGGAAAACG TGCTCACCTT TTACTGCCAG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26218
Clone ID Related Accession (Same CDS sequence) NM_000339
Accession Version NM_000339.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315298.1, U44128.1, AC012181.8 and BF510426.1. This sequence is a reference standard in the RefSeqGene project. On Apr 29, 2008 this sequence version replaced gi:4506976. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U44128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG
AAGGAAGCCA GCGCCAGAGG TGCCAGGCCA TCAGTCTCTG GCGCTTTGGA CCCCAAGGCC
CTGGTGAAGG AGGAGCAGGC CACCACCATC TTCCAGTCGG AGCAGGGCAA GAAGACCATA
GACATCTACT GGCTCTTTGA CGATGGAGGC CTCACCCTCC TCATTCCCTA TCTCCTTGGC
CGCAAGAGGA GGTGGAGCAA ATGCAAGATC CGTGTGTTCG TAGGCGGCCA GATTAACAGG
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGACT GGGATTCCAT
GAAGTCCACA TCCTCCCTGA CATCAACCAG AACCCTCGGG CTGAGCACAC CAAGAGGTTT
GAGGACATGA TTGCACCCTT CCGTCTGAAT GATGGCTTCA AGGATGAGGC CACTGTCAAC
GAGATGCGGC GGGACTGCCC CTGGAAGATC TCAGATGAGG AGATTACGAA GAACAGAGTC
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTGTGCTGG ATTACTCCCG AGACGCTGCT
CTCATCGTCA TCACTTTGCC CATAGGGAGG AAGGGGAAGT GCCCCAGCTC GCTGTACATG
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC
CAGGAAAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000330.2
CDS30..3122
Misc Feature(1)159..410(+)
Misc Feature(2)204..3116(+)
Misc Feature(3)435..497(+)
Misc Feature(4)504..566(+)
Misc Feature(5)684..746(+)
Misc Feature(6)813..875(+)
Misc Feature(7)888..950(+)
Misc Feature(8)1047..1109(+)
Misc Feature(9)1161..1223(+)
Misc Feature(10)1386..1448(+)
Misc Feature(11)1563..1625(+)
Misc Feature(12)1632..1694(+)
Misc Feature(13)1761..1823(+)
Misc Feature(14)2010..2072(+)
Exon (1)1..311
Gene:SLC12A3
Gene Synonym:
Exon (2)312..458
Gene:SLC12A3
Gene Synonym:
Exon (3)459..534
Gene:SLC12A3
Gene Synonym:
Exon (4)535..630
Gene:SLC12A3
Gene Synonym:
Exon (5)631..770
Gene:SLC12A3
Gene Synonym:
Exon (6)771..881
Gene:SLC12A3
Gene Synonym:
Exon (7)882..993
Gene:SLC12A3
Gene Synonym:
Exon (8)994..1124
Gene:SLC12A3
Gene Synonym:
Exon (9)1125..1209
Gene:SLC12A3
Gene Synonym:
Exon (10)1210..1364
Gene:SLC12A3
Gene Synonym:
Exon (11)1365..1472
Gene:SLC12A3
Gene Synonym:
Exon (12)1473..1596
Gene:SLC12A3
Gene Synonym:
Exon (13)1597..1698
Gene:SLC12A3
Gene Synonym:
Exon (14)1699..1854
Gene:SLC12A3
Gene Synonym:
Exon (15)1855..1954
Gene:SLC12A3
Gene Synonym:
Exon (16)1955..2066
Gene:SLC12A3
Gene Synonym:
Exon (17)2067..2207
Gene:SLC12A3
Gene Synonym:
Exon (18)2208..2314
Gene:SLC12A3
Gene Synonym:
Exon (19)2315..2397
Gene:SLC12A3
Gene Synonym:
Exon (20)2398..2475
Gene:SLC12A3
Gene Synonym:
Exon (21)2476..2577
Gene:SLC12A3
Gene Synonym:
Exon (22)2578..2689
Gene:SLC12A3
Gene Synonym:
Exon (23)2690..2776
Gene:SLC12A3
Gene Synonym:
Exon (24)2777..2912
Gene:SLC12A3
Gene Synonym:
Exon (25)2913..2980
Gene:SLC12A3
Gene Synonym:
Exon (26)2981..5567
Gene:SLC12A3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000339
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000339

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG
AAGGAAGCCA GCGCCAGAGG TGCCAGGCCA TCAGTCTCTG GCGCTTTGGA CCCCAAGGCC
CTGGTGAAGG AGGAGCAGGC CACCACCATC TTCCAGTCGG AGCAGGGCAA GAAGACCATA
GACATCTACT GGCTCTTTGA CGATGGAGGC CTCACCCTCC TCATTCCCTA TCTCCTTGGC
CGCAAGAGGA GGTGGAGCAA ATGCAAGATC CGTGTGTTCG TAGGCGGCCA GATTAACAGG
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGACT GGGATTCCAT
GAAGTCCACA TCCTCCCTGA CATCAACCAG AACCCTCGGG CTGAGCACAC CAAGAGGTTT
GAGGACATGA TTGCACCCTT CCGTCTGAAT GATGGCTTCA AGGATGAGGC CACTGTCAAC
GAGATGCGGC GGGACTGCCC CTGGAAGATC TCAGATGAGG AGATTACGAA GAACAGAGTC
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTGTGCTGG ATTACTCCCG AGACGCTGCT
CTCATCGTCA TCACTTTGCC CATAGGGAGG AAGGGGAAGT GCCCCAGCTC GCTGTACATG
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC
CAGGAAAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26776
Clone ID Related Accession (Same CDS sequence) NM_001126107
Accession Version NM_001126107.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315298.1, U44128.1, AC012181.8 and BF510426.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a protein (isoform 2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC
ATCATTGGCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG
GAAGCCAGCG CCAGAGGTGC CAGGCCATCA GTCTCTGGCG CTTTGGACCC CAAGGCCCTG
GTGAAGGAGG AGCAGGCCAC CACCATCTTC CAGTCGGAGC AGGGCAAGAA GACCATAGAC
ATCTACTGGC TCTTTGACGA TGGAGGCCTC ACCCTCCTCA TTCCCTATCT CCTTGGCCGC
AAGAGGAGGT GGAGCAAATG CAAGATCCGT GTGTTCGTAG GCGGCCAGAT TAACAGGATG
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGACTGGG ATTCCATGAA
GTCCACATCC TCCCTGACAT CAACCAGAAC CCTCGGGCTG AGCACACCAA GAGGTTTGAG
GACATGATTG CACCCTTCCG TCTGAATGAT GGCTTCAAGG ATGAGGCCAC TGTCAACGAG
ATGCGGCGGG ACTGCCCCTG GAAGATCTCA GATGAGGAGA TTACGAAGAA CAGAGTCAAG
TCCCTTCGGC AGGTGAGGCT GAATGAGATT GTGCTGGATT ACTCCCGAGA CGCTGCTCTC
ATCGTCATCA CTTTGCCCAT AGGGAGGAAG GGGAAGTGCC CCAGCTCGCT GTACATGGCC
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG
GAAAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119579.1
CDS30..3119
Misc Feature(1)159..383(+)
Misc Feature(2)204..3113(+)
Misc Feature(3)432..494(+)
Misc Feature(4)501..563(+)
Misc Feature(5)681..743(+)
Misc Feature(6)810..872(+)
Misc Feature(7)885..947(+)
Misc Feature(8)1044..1106(+)
Misc Feature(9)1158..1220(+)
Misc Feature(10)1383..1445(+)
Misc Feature(11)1560..1622(+)
Misc Feature(12)1629..1691(+)
Misc Feature(13)1758..1820(+)
Misc Feature(14)2007..2069(+)
Exon (1)1..311
Gene:SLC12A3
Gene Synonym:
Exon (2)312..455
Gene:SLC12A3
Gene Synonym:
Exon (3)456..531
Gene:SLC12A3
Gene Synonym:
Exon (4)532..627
Gene:SLC12A3
Gene Synonym:
Exon (5)628..767
Gene:SLC12A3
Gene Synonym:
Exon (6)768..878
Gene:SLC12A3
Gene Synonym:
Exon (7)879..990
Gene:SLC12A3
Gene Synonym:
Exon (8)991..1121
Gene:SLC12A3
Gene Synonym:
Exon (9)1122..1206
Gene:SLC12A3
Gene Synonym:
Exon (10)1207..1361
Gene:SLC12A3
Gene Synonym:
Exon (11)1362..1469
Gene:SLC12A3
Gene Synonym:
Exon (12)1470..1593
Gene:SLC12A3
Gene Synonym:
Exon (13)1594..1695
Gene:SLC12A3
Gene Synonym:
Exon (14)1696..1851
Gene:SLC12A3
Gene Synonym:
Exon (15)1852..1951
Gene:SLC12A3
Gene Synonym:
Exon (16)1952..2063
Gene:SLC12A3
Gene Synonym:
Exon (17)2064..2204
Gene:SLC12A3
Gene Synonym:
Exon (18)2205..2311
Gene:SLC12A3
Gene Synonym:
Exon (19)2312..2394
Gene:SLC12A3
Gene Synonym:
Exon (20)2395..2472
Gene:SLC12A3
Gene Synonym:
Exon (21)2473..2574
Gene:SLC12A3
Gene Synonym:
Exon (22)2575..2686
Gene:SLC12A3
Gene Synonym:
Exon (23)2687..2773
Gene:SLC12A3
Gene Synonym:
Exon (24)2774..2909
Gene:SLC12A3
Gene Synonym:
Exon (25)2910..2977
Gene:SLC12A3
Gene Synonym:
Exon (26)2978..5564
Gene:SLC12A3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126107
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126107

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC
ATCATTGGCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG
GAAGCCAGCG CCAGAGGTGC CAGGCCATCA GTCTCTGGCG CTTTGGACCC CAAGGCCCTG
GTGAAGGAGG AGCAGGCCAC CACCATCTTC CAGTCGGAGC AGGGCAAGAA GACCATAGAC
ATCTACTGGC TCTTTGACGA TGGAGGCCTC ACCCTCCTCA TTCCCTATCT CCTTGGCCGC
AAGAGGAGGT GGAGCAAATG CAAGATCCGT GTGTTCGTAG GCGGCCAGAT TAACAGGATG
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGACTGGG ATTCCATGAA
GTCCACATCC TCCCTGACAT CAACCAGAAC CCTCGGGCTG AGCACACCAA GAGGTTTGAG
GACATGATTG CACCCTTCCG TCTGAATGAT GGCTTCAAGG ATGAGGCCAC TGTCAACGAG
ATGCGGCGGG ACTGCCCCTG GAAGATCTCA GATGAGGAGA TTACGAAGAA CAGAGTCAAG
TCCCTTCGGC AGGTGAGGCT GAATGAGATT GTGCTGGATT ACTCCCGAGA CGCTGCTCTC
ATCGTCATCA CTTTGCCCAT AGGGAGGAAG GGGAAGTGCC CCAGCTCGCT GTACATGGCC
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG
GAAAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26429
Clone ID Related Accession (Same CDS sequence) NM_001126108
Accession Version NM_001126108.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3066bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 3 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315298.1, X91220.1, AC012181.8 and BF510426.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a protein (isoform 3) that is 9 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X91220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG
AAGGAAGCCA GCGCCAGAGT GGACCCCAAG GCCCTGGTGA AGGAGGAGCA GGCCACCACC
ATCTTCCAGT CGGAGCAGGG CAAGAAGACC ATAGACATCT ACTGGCTCTT TGACGATGGA
GGCCTCACCC TCCTCATTCC CTATCTCCTT GGCCGCAAGA GGAGGTGGAG CAAATGCAAG
ATCCGTGTGT TCGTAGGCGG CCAGATTAAC AGGATGGACC AGGAGAGAAA GGCGATCATT
TCTCTGCTGA GCAAGTTCCG ACTGGGATTC CATGAAGTCC ACATCCTCCC TGACATCAAC
CAGAACCCTC GGGCTGAGCA CACCAAGAGG TTTGAGGACA TGATTGCACC CTTCCGTCTG
AATGATGGCT TCAAGGATGA GGCCACTGTC AACGAGATGC GGCGGGACTG CCCCTGGAAG
ATCTCAGATG AGGAGATTAC GAAGAACAGA GTCAAGTCCC TTCGGCAGGT GAGGCTGAAT
GAGATTGTGC TGGATTACTC CCGAGACGCT GCTCTCATCG TCATCACTTT GCCCATAGGG
AGGAAGGGGA AGTGCCCCAG CTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC
CTCAGACCTC CAGTCATCCT GATCCGAGGA AACCAGGAAA ACGTGCTCAC CTTTTACTGC
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119580.1
CDS30..3095
Misc Feature(1)159..410(+)
Misc Feature(2)204..3089(+)
Misc Feature(3)435..497(+)
Misc Feature(4)504..566(+)
Misc Feature(5)684..746(+)
Misc Feature(6)813..875(+)
Misc Feature(7)888..950(+)
Misc Feature(8)1047..1109(+)
Misc Feature(9)1161..1223(+)
Misc Feature(10)1386..1448(+)
Misc Feature(11)1563..1625(+)
Misc Feature(12)1632..1694(+)
Misc Feature(13)1761..1823(+)
Misc Feature(14)2010..2072(+)
Exon (1)1..311
Gene:SLC12A3
Gene Synonym:
Exon (2)312..458
Gene:SLC12A3
Gene Synonym:
Exon (3)459..534
Gene:SLC12A3
Gene Synonym:
Exon (4)535..630
Gene:SLC12A3
Gene Synonym:
Exon (5)631..770
Gene:SLC12A3
Gene Synonym:
Exon (6)771..881
Gene:SLC12A3
Gene Synonym:
Exon (7)882..993
Gene:SLC12A3
Gene Synonym:
Exon (8)994..1124
Gene:SLC12A3
Gene Synonym:
Exon (9)1125..1209
Gene:SLC12A3
Gene Synonym:
Exon (10)1210..1364
Gene:SLC12A3
Gene Synonym:
Exon (11)1365..1472
Gene:SLC12A3
Gene Synonym:
Exon (12)1473..1596
Gene:SLC12A3
Gene Synonym:
Exon (13)1597..1698
Gene:SLC12A3
Gene Synonym:
Exon (14)1699..1854
Gene:SLC12A3
Gene Synonym:
Exon (15)1855..1954
Gene:SLC12A3
Gene Synonym:
Exon (16)1955..2066
Gene:SLC12A3
Gene Synonym:
Exon (17)2067..2207
Gene:SLC12A3
Gene Synonym:
Exon (18)2208..2314
Gene:SLC12A3
Gene Synonym:
Exon (19)2315..2397
Gene:SLC12A3
Gene Synonym:
Exon (20)2398..2448
Gene:SLC12A3
Gene Synonym:
Exon (21)2449..2550
Gene:SLC12A3
Gene Synonym:
Exon (22)2551..2662
Gene:SLC12A3
Gene Synonym:
Exon (23)2663..2749
Gene:SLC12A3
Gene Synonym:
Exon (24)2750..2885
Gene:SLC12A3
Gene Synonym:
Exon (25)2886..2953
Gene:SLC12A3
Gene Synonym:
Exon (26)2954..5540
Gene:SLC12A3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126108
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126108

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG
AAGGAAGCCA GCGCCAGAGT GGACCCCAAG GCCCTGGTGA AGGAGGAGCA GGCCACCACC
ATCTTCCAGT CGGAGCAGGG CAAGAAGACC ATAGACATCT ACTGGCTCTT TGACGATGGA
GGCCTCACCC TCCTCATTCC CTATCTCCTT GGCCGCAAGA GGAGGTGGAG CAAATGCAAG
ATCCGTGTGT TCGTAGGCGG CCAGATTAAC AGGATGGACC AGGAGAGAAA GGCGATCATT
TCTCTGCTGA GCAAGTTCCG ACTGGGATTC CATGAAGTCC ACATCCTCCC TGACATCAAC
CAGAACCCTC GGGCTGAGCA CACCAAGAGG TTTGAGGACA TGATTGCACC CTTCCGTCTG
AATGATGGCT TCAAGGATGA GGCCACTGTC AACGAGATGC GGCGGGACTG CCCCTGGAAG
ATCTCAGATG AGGAGATTAC GAAGAACAGA GTCAAGTCCC TTCGGCAGGT GAGGCTGAAT
GAGATTGTGC TGGATTACTC CCGAGACGCT GCTCTCATCG TCATCACTTT GCCCATAGGG
AGGAAGGGGA AGTGCCCCAG CTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC
CTCAGACCTC CAGTCATCCT GATCCGAGGA AACCAGGAAA ACGTGCTCAC CTTTTACTGC
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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