SLC12A4 cDNA ORF clone, Homo sapiens(human)

The following SLC12A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09118 NM_001145963.2
Latest version!
Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu09135 NM_001145964.2
Latest version!
Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $741.30
$1059.00
OHu09216 NM_001145961.2
Latest version!
Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu17960 NM_005072.5
Latest version!
Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu09056 NM_001145962.1
Latest version!
Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $769.30
$1099.00
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OHu17960 NM_005072.4 Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu09216 NM_001145961.1 Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu09135 NM_001145964.1 Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $741.30
$1059.00
OHu09118 NM_001145963.1 Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu09118
    Clone ID Related Accession (Same CDS sequence) NM_001145963.2 , NM_001145963.1
    Accession Version NM_001145963.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402650.1, AK316415.1, CF272417.1, AK310027.1 and AI368074.1. On May 31, 2019 this sequence version replaced NM_001145963.1. Transcript Variant: This variant (4) contains an alternate 5' exon which includes a translational start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.261650.1, AK302790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCCG AAGGTGCTGT GTGCGGCTTC GTCTATCTGG AGGGCACTGC CTGGGCGGTC 
    CCTGAGGACA CCGAACCCTT GGCATCCTGC ACATTGGGAC ATGGCAACCA CAGAGAGAGC
    AGCCCTTTTC TTTCCCCCTT GGAGGCTTCC AGAGGAATTG ACTACTATGA CAGGAACCTG
    GCACTGTTTG AGGAAGAGCT GGACATCCGC CCAAAGGTAT CGTCTCTTCT GGGAAAGCTC
    GTCAGCTACA CCAACCTCAC CCAGGGCGCC AAAGAGCATG AGGAGGCCGA GAGTGGGGAG
    GGCACCCGCC GGAGGGCAGC CGAGGCACCC AGCATGGGCA CCCTCATGGG GGTGTACCTG
    CCCTGCCTGC AGAATATCTT TGGGGTTATC CTCTTCCTGC GGCTGACCTG GATGGTGGGC
    ACAGCAGGTG TGCTACAGGC CCTCCTCATC GTGCTTATCT GCTGCTGTTG TACCCTGCTG
    ACGGCCATCT CCATGAGTGC CATCGCCACC AACGGTGTGG TTCCAGCTGG GGGCTCCTAT
    TTCATGATCT CTCGTTCACT GGGGCCAGAA TTTGGAGGTG CTGTGGGCCT GTGCTTCTAC
    CTGGGAACAA CATTCGCAGC AGCCATGTAC ATCCTGGGGG CCATCGAGAT CTTGCTGACC
    TACATTGCCC CACCAGCTGC CATTTTTTAC CCATCGGGTG CTCATGACAC GTCGAATGCC
    ACTTTGAACA ATATGCGTGT GTATGGGACC ATTTTCCTGA CCTTCATGAC CCTGGTGGTG
    TTTGTGGGGG TCAAGTATGT GAACAAATTT GCCTCGCTCT TCCTGGCCTG TGTGATCATC
    TCCATCCTCT CCATCTATGC TGGGGGCATA AAGTCTATAT TTGACCCTCC CGTGTTTCCG
    GTATGCATGC TGGGCAACAG GACCCTGTCC CGGGACCAGT TTGACATCTG TGCCAAGACA
    GCTGTAGTGG ACAATGAGAC AGTGGCCACC CAGCTATGGA GTTTCTTCTG CCACAGCCCC
    AACCTTACGA CCGACTCCTG TGACCCCTAC TTCATGCTCA ACAATGTGAC CGAGATCCCT
    GGCATCCCCG GGGCAGCTGC TGGTGTGCTC CAGGAAAACC TGTGGAGCGC CTACCTGGAG
    AAGGGTGACA TCGTGGAGAA GCATGGGCTG CCCTCCGCAG ATGCCCCGAG CCTGAAGGAG
    AGCCTGCCTC TGTACGTGGT CGCTGACATC GCCACATCCT TCACCGTGCT GGTCGGCATC
    TTCTTCCCTT CTGTAACAGG CATCATGGCT GGCTCAAACC GCTCTGGGGA CCTTCGTGAC
    GCCCAGAAGT CTATCCCTGT GGGGACCATT CTGGCCATCA TTACAACTTC CCTCGTGTAC
    TTCAGCAGTG TGGTTCTCTT TGGTGCCTGC ATTGAGGGTG TGGTTCTCCG GGACAAGTAT
    GGCGATGGTG TCAGCAGGAA CTTGGTGGTG GGCACACTGG CCTGGCCTTC ACCCTGGGTC
    ATCGTCATCG GCTCCTTCTT TTCAACGTGT GGCGCTGGCC TCCAGAGCCT CACAGGGGCA
    CCACGCCTAT TGCAGGCCAT TGCCAAGGAC AACATCATCC CCTTCCTCCG GGTGTTTGGC
    CACGGGAAGG TGAATGGTGA ACCCACATGG GCACTCCTCC TGACGGCACT CATCGCCGAG
    CTGGGCATCC TCATCGCCTC CCTCGACATG GTGGCCCCCA TCTTATCCAT GTTCTTTCTG
    ATGTGCTACC TGTTCGTGAA CCTCGCCTGT GCGGTGCAGA CACTCCTGAG GACCCCCAAC
    TGGCGGCCCC GGTTCAAGTA CTATCACTGG GCGCTGTCCT TCCTGGGCAT GAGTCTCTGC
    CTGGCCCTTA TGTTTGTCTC CTCCTGGTAC TATGCCCTGG TGGCCATGCT CATCGCCGGC
    ATGATCTACA AATACATCGA GTACCAAGGG GCTGAGAAGG AGTGGGGTGA CGGGATCCGA
    GGCCTGTCCC TGAGCGCTGC CCGCTACGCG CTGTTGCGGC TGGAGGAGGG GCCTCCTCAC
    ACCAAGAACT GGCGGCCGCA GCTGCTGGTG CTGCTGAAGC TGGACGAGGA CCTCCACGTG
    AAGTACCCGC GGCTCCTCAC CTTCGCCTCC CAGCTCAAGG CTGGCAAGGG CCTGACCATT
    GTTGGTTCTG TCATCCAGGG GAGCTTCTTG GAGAGCTATG GCGAGGCTCA GGCCGCCGAG
    CAGACCATCA AGAACATGAT GGAAATTGAG AAGGTGAAGG GCTTCTGCCA GGTGGTGGTG
    GCCAGCAAGG TGCGGGAGGG GCTGGCCCAC CTCATCCAGT CCTGTGGCCT GGGAGGCATG
    CGGCATAACT CCGTGGTGCT GGGCTGGCCC TACGGCTGGC GACAGAGCGA GGACCCCCGT
    GCCTGGAAGA CCTTCATTGA CACCGTGCGC TGCACTACGG CTGCCCACCT GGCCCTGCTC
    GTGCCCAAGA ACATCGCCTT CTACCCCAGC AACCACGAGC GCTACCTGGA GGGCCACATA
    GACGTGTGGT GGATCGTGCA CGATGGTGGC ATGCTCATGC TTCTGCCCTT CCTGCTGCGC
    CAGCATAAGG TCTGGAGGAA GTGCCGGATG CGCATCTTCA CAGTGGCCCA GATGGATGAC
    AACAGCATCC AGATGAAGAA GGACCTGGCT GTCTTTCTGT ACCATCTGCG CCTTGAGGCC
    GAGGTGGAGG TGGTGGAGAT GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139435.1
    CDS26..3265
    Translation

    Target ORF information:

    RefSeq Version NM_001145963.2
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145963.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCCG AAGGTGCTGT GTGCGGCTTC GTCTATCTGG AGGGCACTGC CTGGGCGGTC 
    CCTGAGGACA CCGAACCCTT GGCATCCTGC ACATTGGGAC ATGGCAACCA CAGAGAGAGC
    AGCCCTTTTC TTTCCCCCTT GGAGGCTTCC AGAGGAATTG ACTACTATGA CAGGAACCTG
    GCACTGTTTG AGGAAGAGCT GGACATCCGC CCAAAGGTAT CGTCTCTTCT GGGAAAGCTC
    GTCAGCTACA CCAACCTCAC CCAGGGCGCC AAAGAGCATG AGGAGGCCGA GAGTGGGGAG
    GGCACCCGCC GGAGGGCAGC CGAGGCACCC AGCATGGGCA CCCTCATGGG GGTGTACCTG
    CCCTGCCTGC AGAATATCTT TGGGGTTATC CTCTTCCTGC GGCTGACCTG GATGGTGGGC
    ACAGCAGGTG TGCTACAGGC CCTCCTCATC GTGCTTATCT GCTGCTGTTG TACCCTGCTG
    ACGGCCATCT CCATGAGTGC CATCGCCACC AACGGTGTGG TTCCAGCTGG GGGCTCCTAT
    TTCATGATCT CTCGTTCACT GGGGCCAGAA TTTGGAGGTG CTGTGGGCCT GTGCTTCTAC
    CTGGGAACAA CATTCGCAGC AGCCATGTAC ATCCTGGGGG CCATCGAGAT CTTGCTGACC
    TACATTGCCC CACCAGCTGC CATTTTTTAC CCATCGGGTG CTCATGACAC GTCGAATGCC
    ACTTTGAACA ATATGCGTGT GTATGGGACC ATTTTCCTGA CCTTCATGAC CCTGGTGGTG
    TTTGTGGGGG TCAAGTATGT GAACAAATTT GCCTCGCTCT TCCTGGCCTG TGTGATCATC
    TCCATCCTCT CCATCTATGC TGGGGGCATA AAGTCTATAT TTGACCCTCC CGTGTTTCCG
    GTATGCATGC TGGGCAACAG GACCCTGTCC CGGGACCAGT TTGACATCTG TGCCAAGACA
    GCTGTAGTGG ACAATGAGAC AGTGGCCACC CAGCTATGGA GTTTCTTCTG CCACAGCCCC
    AACCTTACGA CCGACTCCTG TGACCCCTAC TTCATGCTCA ACAATGTGAC CGAGATCCCT
    GGCATCCCCG GGGCAGCTGC TGGTGTGCTC CAGGAAAACC TGTGGAGCGC CTACCTGGAG
    AAGGGTGACA TCGTGGAGAA GCATGGGCTG CCCTCCGCAG ATGCCCCGAG CCTGAAGGAG
    AGCCTGCCTC TGTACGTGGT CGCTGACATC GCCACATCCT TCACCGTGCT GGTCGGCATC
    TTCTTCCCTT CTGTAACAGG CATCATGGCT GGCTCAAACC GCTCTGGGGA CCTTCGTGAC
    GCCCAGAAGT CTATCCCTGT GGGGACCATT CTGGCCATCA TTACAACTTC CCTCGTGTAC
    TTCAGCAGTG TGGTTCTCTT TGGTGCCTGC ATTGAGGGTG TGGTTCTCCG GGACAAGTAT
    GGCGATGGTG TCAGCAGGAA CTTGGTGGTG GGCACACTGG CCTGGCCTTC ACCCTGGGTC
    ATCGTCATCG GCTCCTTCTT TTCAACGTGT GGCGCTGGCC TCCAGAGCCT CACAGGGGCA
    CCACGCCTAT TGCAGGCCAT TGCCAAGGAC AACATCATCC CCTTCCTCCG GGTGTTTGGC
    CACGGGAAGG TGAATGGTGA ACCCACATGG GCACTCCTCC TGACGGCACT CATCGCCGAG
    CTGGGCATCC TCATCGCCTC CCTCGACATG GTGGCCCCCA TCTTATCCAT GTTCTTTCTG
    ATGTGCTACC TGTTCGTGAA CCTCGCCTGT GCGGTGCAGA CACTCCTGAG GACCCCCAAC
    TGGCGGCCCC GGTTCAAGTA CTATCACTGG GCGCTGTCCT TCCTGGGCAT GAGTCTCTGC
    CTGGCCCTTA TGTTTGTCTC CTCCTGGTAC TATGCCCTGG TGGCCATGCT CATCGCCGGC
    ATGATCTACA AATACATCGA GTACCAAGGG GCTGAGAAGG AGTGGGGTGA CGGGATCCGA
    GGCCTGTCCC TGAGCGCTGC CCGCTACGCG CTGTTGCGGC TGGAGGAGGG GCCTCCTCAC
    ACCAAGAACT GGCGGCCGCA GCTGCTGGTG CTGCTGAAGC TGGACGAGGA CCTCCACGTG
    AAGTACCCGC GGCTCCTCAC CTTCGCCTCC CAGCTCAAGG CTGGCAAGGG CCTGACCATT
    GTTGGTTCTG TCATCCAGGG GAGCTTCTTG GAGAGCTATG GCGAGGCTCA GGCCGCCGAG
    CAGACCATCA AGAACATGAT GGAAATTGAG AAGGTGAAGG GCTTCTGCCA GGTGGTGGTG
    GCCAGCAAGG TGCGGGAGGG GCTGGCCCAC CTCATCCAGT CCTGTGGCCT GGGAGGCATG
    CGGCATAACT CCGTGGTGCT GGGCTGGCCC TACGGCTGGC GACAGAGCGA GGACCCCCGT
    GCCTGGAAGA CCTTCATTGA CACCGTGCGC TGCACTACGG CTGCCCACCT GGCCCTGCTC
    GTGCCCAAGA ACATCGCCTT CTACCCCAGC AACCACGAGC GCTACCTGGA GGGCCACATA
    GACGTGTGGT GGATCGTGCA CGATGGTGGC ATGCTCATGC TTCTGCCCTT CCTGCTGCGC
    CAGCATAAGG TCTGGAGGAA GTGCCGGATG CGCATCTTCA CAGTGGCCCA GATGGATGAC
    AACAGCATCC AGATGAAGAA GGACCTGGCT GTCTTTCTGT ACCATCTGCG CCTTGAGGCC
    GAGGTGGAGG TGGTGGAGAT GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09118
    Clone ID Related Accession (Same CDS sequence) NM_001145963.2 , NM_001145963.1
    Accession Version NM_001145963.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-24
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402650.1, AK316415.1, CF272417.1, AK310027.1 and AI368074.1. Transcript Variant: This variant (4) contains an alternate 5' exon which includes a translational start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302790.1, SRR1803612.261650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCCG AAGGTGCTGT GTGCGGCTTC GTCTATCTGG AGGGCACTGC CTGGGCGGTC 
    CCTGAGGACA CCGAACCCTT GGCATCCTGC ACATTGGGAC ATGGCAACCA CAGAGAGAGC
    AGCCCTTTTC TTTCCCCCTT GGAGGCTTCC AGAGGAATTG ACTACTATGA CAGGAACCTG
    GCACTGTTTG AGGAAGAGCT GGACATCCGC CCAAAGGTAT CGTCTCTTCT GGGAAAGCTC
    GTCAGCTACA CCAACCTCAC CCAGGGCGCC AAAGAGCATG AGGAGGCCGA GAGTGGGGAG
    GGCACCCGCC GGAGGGCAGC CGAGGCACCC AGCATGGGCA CCCTCATGGG GGTGTACCTG
    CCCTGCCTGC AGAATATCTT TGGGGTTATC CTCTTCCTGC GGCTGACCTG GATGGTGGGC
    ACAGCAGGTG TGCTACAGGC CCTCCTCATC GTGCTTATCT GCTGCTGTTG TACCCTGCTG
    ACGGCCATCT CCATGAGTGC CATCGCCACC AACGGTGTGG TTCCAGCTGG GGGCTCCTAT
    TTCATGATCT CTCGTTCACT GGGGCCAGAA TTTGGAGGTG CTGTGGGCCT GTGCTTCTAC
    CTGGGAACAA CATTCGCAGC AGCCATGTAC ATCCTGGGGG CCATCGAGAT CTTGCTGACC
    TACATTGCCC CACCAGCTGC CATTTTTTAC CCATCGGGTG CTCATGACAC GTCGAATGCC
    ACTTTGAACA ATATGCGTGT GTATGGGACC ATTTTCCTGA CCTTCATGAC CCTGGTGGTG
    TTTGTGGGGG TCAAGTATGT GAACAAATTT GCCTCGCTCT TCCTGGCCTG TGTGATCATC
    TCCATCCTCT CCATCTATGC TGGGGGCATA AAGTCTATAT TTGACCCTCC CGTGTTTCCG
    GTATGCATGC TGGGCAACAG GACCCTGTCC CGGGACCAGT TTGACATCTG TGCCAAGACA
    GCTGTAGTGG ACAATGAGAC AGTGGCCACC CAGCTATGGA GTTTCTTCTG CCACAGCCCC
    AACCTTACGA CCGACTCCTG TGACCCCTAC TTCATGCTCA ACAATGTGAC CGAGATCCCT
    GGCATCCCCG GGGCAGCTGC TGGTGTGCTC CAGGAAAACC TGTGGAGCGC CTACCTGGAG
    AAGGGTGACA TCGTGGAGAA GCATGGGCTG CCCTCCGCAG ATGCCCCGAG CCTGAAGGAG
    AGCCTGCCTC TGTACGTGGT CGCTGACATC GCCACATCCT TCACCGTGCT GGTCGGCATC
    TTCTTCCCTT CTGTAACAGG CATCATGGCT GGCTCAAACC GCTCTGGGGA CCTTCGTGAC
    GCCCAGAAGT CTATCCCTGT GGGGACCATT CTGGCCATCA TTACAACTTC CCTCGTGTAC
    TTCAGCAGTG TGGTTCTCTT TGGTGCCTGC ATTGAGGGTG TGGTTCTCCG GGACAAGTAT
    GGCGATGGTG TCAGCAGGAA CTTGGTGGTG GGCACACTGG CCTGGCCTTC ACCCTGGGTC
    ATCGTCATCG GCTCCTTCTT TTCAACGTGT GGCGCTGGCC TCCAGAGCCT CACAGGGGCA
    CCACGCCTAT TGCAGGCCAT TGCCAAGGAC AACATCATCC CCTTCCTCCG GGTGTTTGGC
    CACGGGAAGG TGAATGGTGA ACCCACATGG GCACTCCTCC TGACGGCACT CATCGCCGAG
    CTGGGCATCC TCATCGCCTC CCTCGACATG GTGGCCCCCA TCTTATCCAT GTTCTTTCTG
    ATGTGCTACC TGTTCGTGAA CCTCGCCTGT GCGGTGCAGA CACTCCTGAG GACCCCCAAC
    TGGCGGCCCC GGTTCAAGTA CTATCACTGG GCGCTGTCCT TCCTGGGCAT GAGTCTCTGC
    CTGGCCCTTA TGTTTGTCTC CTCCTGGTAC TATGCCCTGG TGGCCATGCT CATCGCCGGC
    ATGATCTACA AATACATCGA GTACCAAGGG GCTGAGAAGG AGTGGGGTGA CGGGATCCGA
    GGCCTGTCCC TGAGCGCTGC CCGCTACGCG CTGTTGCGGC TGGAGGAGGG GCCTCCTCAC
    ACCAAGAACT GGCGGCCGCA GCTGCTGGTG CTGCTGAAGC TGGACGAGGA CCTCCACGTG
    AAGTACCCGC GGCTCCTCAC CTTCGCCTCC CAGCTCAAGG CTGGCAAGGG CCTGACCATT
    GTTGGTTCTG TCATCCAGGG GAGCTTCTTG GAGAGCTATG GCGAGGCTCA GGCCGCCGAG
    CAGACCATCA AGAACATGAT GGAAATTGAG AAGGTGAAGG GCTTCTGCCA GGTGGTGGTG
    GCCAGCAAGG TGCGGGAGGG GCTGGCCCAC CTCATCCAGT CCTGTGGCCT GGGAGGCATG
    CGGCATAACT CCGTGGTGCT GGGCTGGCCC TACGGCTGGC GACAGAGCGA GGACCCCCGT
    GCCTGGAAGA CCTTCATTGA CACCGTGCGC TGCACTACGG CTGCCCACCT GGCCCTGCTC
    GTGCCCAAGA ACATCGCCTT CTACCCCAGC AACCACGAGC GCTACCTGGA GGGCCACATA
    GACGTGTGGT GGATCGTGCA CGATGGTGGC ATGCTCATGC TTCTGCCCTT CCTGCTGCGC
    CAGCATAAGG TCTGGAGGAA GTGCCGGATG CGCATCTTCA CAGTGGCCCA GATGGATGAC
    AACAGCATCC AGATGAAGAA GGACCTGGCT GTCTTTCTGT ACCATCTGCG CCTTGAGGCC
    GAGGTGGAGG TGGTGGAGAT GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139435.1
    CDS39..3278
    Translation

    Target ORF information:

    RefSeq Version NM_001145963.1
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145963.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCCG AAGGTGCTGT GTGCGGCTTC GTCTATCTGG AGGGCACTGC CTGGGCGGTC 
    CCTGAGGACA CCGAACCCTT GGCATCCTGC ACATTGGGAC ATGGCAACCA CAGAGAGAGC
    AGCCCTTTTC TTTCCCCCTT GGAGGCTTCC AGAGGAATTG ACTACTATGA CAGGAACCTG
    GCACTGTTTG AGGAAGAGCT GGACATCCGC CCAAAGGTAT CGTCTCTTCT GGGAAAGCTC
    GTCAGCTACA CCAACCTCAC CCAGGGCGCC AAAGAGCATG AGGAGGCCGA GAGTGGGGAG
    GGCACCCGCC GGAGGGCAGC CGAGGCACCC AGCATGGGCA CCCTCATGGG GGTGTACCTG
    CCCTGCCTGC AGAATATCTT TGGGGTTATC CTCTTCCTGC GGCTGACCTG GATGGTGGGC
    ACAGCAGGTG TGCTACAGGC CCTCCTCATC GTGCTTATCT GCTGCTGTTG TACCCTGCTG
    ACGGCCATCT CCATGAGTGC CATCGCCACC AACGGTGTGG TTCCAGCTGG GGGCTCCTAT
    TTCATGATCT CTCGTTCACT GGGGCCAGAA TTTGGAGGTG CTGTGGGCCT GTGCTTCTAC
    CTGGGAACAA CATTCGCAGC AGCCATGTAC ATCCTGGGGG CCATCGAGAT CTTGCTGACC
    TACATTGCCC CACCAGCTGC CATTTTTTAC CCATCGGGTG CTCATGACAC GTCGAATGCC
    ACTTTGAACA ATATGCGTGT GTATGGGACC ATTTTCCTGA CCTTCATGAC CCTGGTGGTG
    TTTGTGGGGG TCAAGTATGT GAACAAATTT GCCTCGCTCT TCCTGGCCTG TGTGATCATC
    TCCATCCTCT CCATCTATGC TGGGGGCATA AAGTCTATAT TTGACCCTCC CGTGTTTCCG
    GTATGCATGC TGGGCAACAG GACCCTGTCC CGGGACCAGT TTGACATCTG TGCCAAGACA
    GCTGTAGTGG ACAATGAGAC AGTGGCCACC CAGCTATGGA GTTTCTTCTG CCACAGCCCC
    AACCTTACGA CCGACTCCTG TGACCCCTAC TTCATGCTCA ACAATGTGAC CGAGATCCCT
    GGCATCCCCG GGGCAGCTGC TGGTGTGCTC CAGGAAAACC TGTGGAGCGC CTACCTGGAG
    AAGGGTGACA TCGTGGAGAA GCATGGGCTG CCCTCCGCAG ATGCCCCGAG CCTGAAGGAG
    AGCCTGCCTC TGTACGTGGT CGCTGACATC GCCACATCCT TCACCGTGCT GGTCGGCATC
    TTCTTCCCTT CTGTAACAGG CATCATGGCT GGCTCAAACC GCTCTGGGGA CCTTCGTGAC
    GCCCAGAAGT CTATCCCTGT GGGGACCATT CTGGCCATCA TTACAACTTC CCTCGTGTAC
    TTCAGCAGTG TGGTTCTCTT TGGTGCCTGC ATTGAGGGTG TGGTTCTCCG GGACAAGTAT
    GGCGATGGTG TCAGCAGGAA CTTGGTGGTG GGCACACTGG CCTGGCCTTC ACCCTGGGTC
    ATCGTCATCG GCTCCTTCTT TTCAACGTGT GGCGCTGGCC TCCAGAGCCT CACAGGGGCA
    CCACGCCTAT TGCAGGCCAT TGCCAAGGAC AACATCATCC CCTTCCTCCG GGTGTTTGGC
    CACGGGAAGG TGAATGGTGA ACCCACATGG GCACTCCTCC TGACGGCACT CATCGCCGAG
    CTGGGCATCC TCATCGCCTC CCTCGACATG GTGGCCCCCA TCTTATCCAT GTTCTTTCTG
    ATGTGCTACC TGTTCGTGAA CCTCGCCTGT GCGGTGCAGA CACTCCTGAG GACCCCCAAC
    TGGCGGCCCC GGTTCAAGTA CTATCACTGG GCGCTGTCCT TCCTGGGCAT GAGTCTCTGC
    CTGGCCCTTA TGTTTGTCTC CTCCTGGTAC TATGCCCTGG TGGCCATGCT CATCGCCGGC
    ATGATCTACA AATACATCGA GTACCAAGGG GCTGAGAAGG AGTGGGGTGA CGGGATCCGA
    GGCCTGTCCC TGAGCGCTGC CCGCTACGCG CTGTTGCGGC TGGAGGAGGG GCCTCCTCAC
    ACCAAGAACT GGCGGCCGCA GCTGCTGGTG CTGCTGAAGC TGGACGAGGA CCTCCACGTG
    AAGTACCCGC GGCTCCTCAC CTTCGCCTCC CAGCTCAAGG CTGGCAAGGG CCTGACCATT
    GTTGGTTCTG TCATCCAGGG GAGCTTCTTG GAGAGCTATG GCGAGGCTCA GGCCGCCGAG
    CAGACCATCA AGAACATGAT GGAAATTGAG AAGGTGAAGG GCTTCTGCCA GGTGGTGGTG
    GCCAGCAAGG TGCGGGAGGG GCTGGCCCAC CTCATCCAGT CCTGTGGCCT GGGAGGCATG
    CGGCATAACT CCGTGGTGCT GGGCTGGCCC TACGGCTGGC GACAGAGCGA GGACCCCCGT
    GCCTGGAAGA CCTTCATTGA CACCGTGCGC TGCACTACGG CTGCCCACCT GGCCCTGCTC
    GTGCCCAAGA ACATCGCCTT CTACCCCAGC AACCACGAGC GCTACCTGGA GGGCCACATA
    GACGTGTGGT GGATCGTGCA CGATGGTGGC ATGCTCATGC TTCTGCCCTT CCTGCTGCGC
    CAGCATAAGG TCTGGAGGAA GTGCCGGATG CGCATCTTCA CAGTGGCCCA GATGGATGAC
    AACAGCATCC AGATGAAGAA GGACCTGGCT GTCTTTCTGT ACCATCTGCG CCTTGAGGCC
    GAGGTGGAGG TGGTGGAGAT GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09135
    Clone ID Related Accession (Same CDS sequence) NM_001145964.2 , NM_001145964.1
    Accession Version NM_001145964.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC333904.1, AK299042.1, CF272417.1, AK310027.1 and AI368074.1. On May 31, 2019 this sequence version replaced NM_001145964.1. Transcript Variant: This variant (5) contains an alternate 5' exon which includes a translational start codon, compared to variant 1. The encoded isoform (e) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299042.1, SRR1803613.136433.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGGACA CCCTGAGCCC CGGACATGGC AACCACAGAG AGAGCAGCCC TTTTCTTTCC 
    CCCTTGGAGG CTTCCAGAGG AATTGACTAC TATGACAGGA ACCTGGCACT GTTTGAGGAA
    GAGCTGGACA TCCGCCCAAA GGTATCGTCT CTTCTGGGAA AGCTCGTCAG CTACACCAAC
    CTCACCCAGG GCGCCAAAGA GCATGAGGAG GCCGAGAGTG GGGAGGGCAC CCGCCGGAGG
    GCAGCCGAGG CACCCAGCAT GGGCACCCTC ATGGGGGTGT ACCTGCCCTG CCTGCAGAAT
    ATCTTTGGGG TTATCCTCTT CCTGCGGCTG ACCTGGATGG TGGGCACAGC AGGTGTGCTA
    CAGGCCCTCC TCATCGTGCT TATCTGCTGC TGTTGTACCC TGCTGACGGC CATCTCCATG
    AGTGCCATCG CCACCAACGG TGTGGTTCCA GCTGGGGGCT CCTATTTCAT GATCTCTCGT
    TCACTGGGGC CAGAATTTGG AGGTGCTGTG GGCCTGTGCT TCTACCTGGG AACAACATTC
    GCAGCAGCCA TGTACATCCT GGGGGCCATC GAGATCTTGC TGACCTACAT TGCCCCACCA
    GCTGCCATTT TTTACCCATC GGGTGCTCAT GACACGTCGA ATGCCACTTT GAACAATATG
    CGTGTGTATG GGACCATTTT CCTGACCTTC ATGACCCTGG TGGTGTTTGT GGGGGTCAAG
    TATGTGAACA AATTTGCCTC GCTCTTCCTG GCCTGTGTGA TCATCTCCAT CCTCTCCATC
    TATGCTGGGG GCATAAAGTC TATATTTGAC CCTCCCGTGT TTCCGGTATG CATGCTGGGC
    AACAGGACCC TGTCCCGGGA CCAGTTTGAC ATCTGTGCCA AGACAGCTGT AGTGGACAAT
    GAGACAGTGG CCACCCAGCT ATGGAGTTTC TTCTGCCACA GCCCCAACCT TACGACCGAC
    TCCTGTGACC CCTACTTCAT GCTCAACAAT GTGACCGAGA TCCCTGGCAT CCCCGGGGCA
    GCTGCTGGTG TGCTCCAGGA AAACCTGTGG AGCGCCTACC TGGAGAAGGG TGACATCGTG
    GAGAAGCATG GGCTGCCCTC CGCAGATGCC CCGAGCCTGA AGGAGAGCCT GCCTCTGTAC
    GTGGTCGCTG ACATCGCCAC ATCCTTCACC GTGCTGGTCG GCATCTTCTT CCCTTCTGTA
    ACAGGCATCA TGGCTGGCTC AAACCGCTCT GGGGACCTTC GTGACGCCCA GAAGTCTATC
    CCTGTGGGGA CCATTCTGGC CATCATTACA ACTTCCCTCG TGTACTTCAG CAGTGTGGTT
    CTCTTTGGTG CCTGCATTGA GGGTGTGGTT CTCCGGGACA AGTATGGCGA TGGTGTCAGC
    AGGAACTTGG TGGTGGGCAC ACTGGCCTGG CCTTCACCCT GGGTCATCGT CATCGGCTCC
    TTCTTTTCAA CGTGTGGCGC TGGCCTCCAG AGCCTCACAG GGGCACCACG CCTATTGCAG
    GCCATTGCCA AGGACAACAT CATCCCCTTC CTCCGGGTGT TTGGCCACGG GAAGGTGAAT
    GGTGAACCCA CATGGGCACT CCTCCTGACG GCACTCATCG CCGAGCTGGG CATCCTCATC
    GCCTCCCTCG ACATGGTGGC CCCCATCTTA TCCATGTTCT TTCTGATGTG CTACCTGTTC
    GTGAACCTCG CCTGTGCGGT GCAGACACTC CTGAGGACCC CCAACTGGCG GCCCCGGTTC
    AAGTACTATC ACTGGGCGCT GTCCTTCCTG GGCATGAGTC TCTGCCTGGC CCTTATGTTT
    GTCTCCTCCT GGTACTATGC CCTGGTGGCC ATGCTCATCG CCGGCATGAT CTACAAATAC
    ATCGAGTACC AAGGGGCTGA GAAGGAGTGG GGTGACGGGA TCCGAGGCCT GTCCCTGAGC
    GCTGCCCGCT ACGCGCTGTT GCGGCTGGAG GAGGGGCCTC CTCACACCAA GAACTGGCGG
    CCGCAGCTGC TGGTGCTGCT GAAGCTGGAC GAGGACCTCC ACGTGAAGTA CCCGCGGCTC
    CTCACCTTCG CCTCCCAGCT CAAGGCTGGC AAGGGCCTGA CCATTGTTGG TTCTGTCATC
    CAGGGGAGCT TCTTGGAGAG CTATGGCGAG GCTCAGGCCG CCGAGCAGAC CATCAAGAAC
    ATGATGGAAA TTGAGAAGGT GAAGGGCTTC TGCCAGGTGG TGGTGGCCAG CAAGGTGCGG
    GAGGGGCTGG CCCACCTCAT CCAGTCCTGT GGCCTGGGAG GCATGCGGCA TAACTCCGTG
    GTGCTGGGCT GGCCCTACGG CTGGCGACAG AGCGAGGACC CCCGTGCCTG GAAGACCTTC
    ATTGACACCG TGCGCTGCAC TACGGCTGCC CACCTGGCCC TGCTCGTGCC CAAGAACATC
    GCCTTCTACC CCAGCAACCA CGAGCGCTAC CTGGAGGGCC ACATAGACGT GTGGTGGATC
    GTGCACGATG GTGGCATGCT CATGCTTCTG CCCTTCCTGC TGCGCCAGCA TAAGGTCTGG
    AGGAAGTGCC GGATGCGCAT CTTCACAGTG GCCCAGATGG ATGACAACAG CATCCAGATG
    AAGAAGGACC TGGCTGTCTT TCTGTACCAT CTGCGCCTTG AGGCCGAGGT GGAGGTGGTG
    GAGATGCATA ACAGTGACAT CTCTGCATAC ACCTACGAGC GGACGCTGAT GATGGAGCAG
    CGGTCGCAGA TGCTGCGGCA GATGAGACTG ACCAAGACTG AGCGGGAGCG AGAAGCCCAG
    CTGGTCAAGG ATCGGCACTC GGCCCTGCGG CTGGAGAGCC TGTACTCGGA CGAGGAAGAT
    GAGTCTGCAG TGGGGGCTGA CAAGATCCAG ATGACGTGGA CCAGGGACAA GTACATGACT
    GAGACCTGGG ACCCCAGCCA TGCCCCTGAC AATTTCCGGG AGCTGGTGCA CATTAAGCCG
    GACCAATCCA ATGTGCGGCG CATGCACACT GCTGTGAAGC TCAATGAAGT CATTGTCACG
    CGCTCCCACG ACGCCCGCCT GGTTCTCCTA AACATGCCTG GCCCACCCAG GAACAGTGAG
    GGCGACGAGA ACTACATGGA GTTCCTCGAG GTGCTGACCG AGGGCCTTGA GCGGGTGCTG
    TTGGTGCGCG GTGGTGGCCG TGAAGTCATC ACCATCTACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139436.1
    CDS21..3185
    Translation

    Target ORF information:

    RefSeq Version NM_001145964.2
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145964.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGGACA CCCTGAGCCC CGGACATGGC AACCACAGAG AGAGCAGCCC TTTTCTTTCC 
    CCCTTGGAGG CTTCCAGAGG AATTGACTAC TATGACAGGA ACCTGGCACT GTTTGAGGAA
    GAGCTGGACA TCCGCCCAAA GGTATCGTCT CTTCTGGGAA AGCTCGTCAG CTACACCAAC
    CTCACCCAGG GCGCCAAAGA GCATGAGGAG GCCGAGAGTG GGGAGGGCAC CCGCCGGAGG
    GCAGCCGAGG CACCCAGCAT GGGCACCCTC ATGGGGGTGT ACCTGCCCTG CCTGCAGAAT
    ATCTTTGGGG TTATCCTCTT CCTGCGGCTG ACCTGGATGG TGGGCACAGC AGGTGTGCTA
    CAGGCCCTCC TCATCGTGCT TATCTGCTGC TGTTGTACCC TGCTGACGGC CATCTCCATG
    AGTGCCATCG CCACCAACGG TGTGGTTCCA GCTGGGGGCT CCTATTTCAT GATCTCTCGT
    TCACTGGGGC CAGAATTTGG AGGTGCTGTG GGCCTGTGCT TCTACCTGGG AACAACATTC
    GCAGCAGCCA TGTACATCCT GGGGGCCATC GAGATCTTGC TGACCTACAT TGCCCCACCA
    GCTGCCATTT TTTACCCATC GGGTGCTCAT GACACGTCGA ATGCCACTTT GAACAATATG
    CGTGTGTATG GGACCATTTT CCTGACCTTC ATGACCCTGG TGGTGTTTGT GGGGGTCAAG
    TATGTGAACA AATTTGCCTC GCTCTTCCTG GCCTGTGTGA TCATCTCCAT CCTCTCCATC
    TATGCTGGGG GCATAAAGTC TATATTTGAC CCTCCCGTGT TTCCGGTATG CATGCTGGGC
    AACAGGACCC TGTCCCGGGA CCAGTTTGAC ATCTGTGCCA AGACAGCTGT AGTGGACAAT
    GAGACAGTGG CCACCCAGCT ATGGAGTTTC TTCTGCCACA GCCCCAACCT TACGACCGAC
    TCCTGTGACC CCTACTTCAT GCTCAACAAT GTGACCGAGA TCCCTGGCAT CCCCGGGGCA
    GCTGCTGGTG TGCTCCAGGA AAACCTGTGG AGCGCCTACC TGGAGAAGGG TGACATCGTG
    GAGAAGCATG GGCTGCCCTC CGCAGATGCC CCGAGCCTGA AGGAGAGCCT GCCTCTGTAC
    GTGGTCGCTG ACATCGCCAC ATCCTTCACC GTGCTGGTCG GCATCTTCTT CCCTTCTGTA
    ACAGGCATCA TGGCTGGCTC AAACCGCTCT GGGGACCTTC GTGACGCCCA GAAGTCTATC
    CCTGTGGGGA CCATTCTGGC CATCATTACA ACTTCCCTCG TGTACTTCAG CAGTGTGGTT
    CTCTTTGGTG CCTGCATTGA GGGTGTGGTT CTCCGGGACA AGTATGGCGA TGGTGTCAGC
    AGGAACTTGG TGGTGGGCAC ACTGGCCTGG CCTTCACCCT GGGTCATCGT CATCGGCTCC
    TTCTTTTCAA CGTGTGGCGC TGGCCTCCAG AGCCTCACAG GGGCACCACG CCTATTGCAG
    GCCATTGCCA AGGACAACAT CATCCCCTTC CTCCGGGTGT TTGGCCACGG GAAGGTGAAT
    GGTGAACCCA CATGGGCACT CCTCCTGACG GCACTCATCG CCGAGCTGGG CATCCTCATC
    GCCTCCCTCG ACATGGTGGC CCCCATCTTA TCCATGTTCT TTCTGATGTG CTACCTGTTC
    GTGAACCTCG CCTGTGCGGT GCAGACACTC CTGAGGACCC CCAACTGGCG GCCCCGGTTC
    AAGTACTATC ACTGGGCGCT GTCCTTCCTG GGCATGAGTC TCTGCCTGGC CCTTATGTTT
    GTCTCCTCCT GGTACTATGC CCTGGTGGCC ATGCTCATCG CCGGCATGAT CTACAAATAC
    ATCGAGTACC AAGGGGCTGA GAAGGAGTGG GGTGACGGGA TCCGAGGCCT GTCCCTGAGC
    GCTGCCCGCT ACGCGCTGTT GCGGCTGGAG GAGGGGCCTC CTCACACCAA GAACTGGCGG
    CCGCAGCTGC TGGTGCTGCT GAAGCTGGAC GAGGACCTCC ACGTGAAGTA CCCGCGGCTC
    CTCACCTTCG CCTCCCAGCT CAAGGCTGGC AAGGGCCTGA CCATTGTTGG TTCTGTCATC
    CAGGGGAGCT TCTTGGAGAG CTATGGCGAG GCTCAGGCCG CCGAGCAGAC CATCAAGAAC
    ATGATGGAAA TTGAGAAGGT GAAGGGCTTC TGCCAGGTGG TGGTGGCCAG CAAGGTGCGG
    GAGGGGCTGG CCCACCTCAT CCAGTCCTGT GGCCTGGGAG GCATGCGGCA TAACTCCGTG
    GTGCTGGGCT GGCCCTACGG CTGGCGACAG AGCGAGGACC CCCGTGCCTG GAAGACCTTC
    ATTGACACCG TGCGCTGCAC TACGGCTGCC CACCTGGCCC TGCTCGTGCC CAAGAACATC
    GCCTTCTACC CCAGCAACCA CGAGCGCTAC CTGGAGGGCC ACATAGACGT GTGGTGGATC
    GTGCACGATG GTGGCATGCT CATGCTTCTG CCCTTCCTGC TGCGCCAGCA TAAGGTCTGG
    AGGAAGTGCC GGATGCGCAT CTTCACAGTG GCCCAGATGG ATGACAACAG CATCCAGATG
    AAGAAGGACC TGGCTGTCTT TCTGTACCAT CTGCGCCTTG AGGCCGAGGT GGAGGTGGTG
    GAGATGCATA ACAGTGACAT CTCTGCATAC ACCTACGAGC GGACGCTGAT GATGGAGCAG
    CGGTCGCAGA TGCTGCGGCA GATGAGACTG ACCAAGACTG AGCGGGAGCG AGAAGCCCAG
    CTGGTCAAGG ATCGGCACTC GGCCCTGCGG CTGGAGAGCC TGTACTCGGA CGAGGAAGAT
    GAGTCTGCAG TGGGGGCTGA CAAGATCCAG ATGACGTGGA CCAGGGACAA GTACATGACT
    GAGACCTGGG ACCCCAGCCA TGCCCCTGAC AATTTCCGGG AGCTGGTGCA CATTAAGCCG
    GACCAATCCA ATGTGCGGCG CATGCACACT GCTGTGAAGC TCAATGAAGT CATTGTCACG
    CGCTCCCACG ACGCCCGCCT GGTTCTCCTA AACATGCCTG GCCCACCCAG GAACAGTGAG
    GGCGACGAGA ACTACATGGA GTTCCTCGAG GTGCTGACCG AGGGCCTTGA GCGGGTGCTG
    TTGGTGCGCG GTGGTGGCCG TGAAGTCATC ACCATCTACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09135
    Clone ID Related Accession (Same CDS sequence) NM_001145964.2 , NM_001145964.1
    Accession Version NM_001145964.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-24
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC333904.1, AK299042.1, CF272417.1, AK310027.1 and AI368074.1. Transcript Variant: This variant (5) contains an alternate 5' exon which includes a translational start codon, compared to variant 1. The encoded isoform (e) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.136433.1, SRR1803617.197310.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGGACA CCCTGAGCCC CGGACATGGC AACCACAGAG AGAGCAGCCC TTTTCTTTCC 
    CCCTTGGAGG CTTCCAGAGG AATTGACTAC TATGACAGGA ACCTGGCACT GTTTGAGGAA
    GAGCTGGACA TCCGCCCAAA GGTATCGTCT CTTCTGGGAA AGCTCGTCAG CTACACCAAC
    CTCACCCAGG GCGCCAAAGA GCATGAGGAG GCCGAGAGTG GGGAGGGCAC CCGCCGGAGG
    GCAGCCGAGG CACCCAGCAT GGGCACCCTC ATGGGGGTGT ACCTGCCCTG CCTGCAGAAT
    ATCTTTGGGG TTATCCTCTT CCTGCGGCTG ACCTGGATGG TGGGCACAGC AGGTGTGCTA
    CAGGCCCTCC TCATCGTGCT TATCTGCTGC TGTTGTACCC TGCTGACGGC CATCTCCATG
    AGTGCCATCG CCACCAACGG TGTGGTTCCA GCTGGGGGCT CCTATTTCAT GATCTCTCGT
    TCACTGGGGC CAGAATTTGG AGGTGCTGTG GGCCTGTGCT TCTACCTGGG AACAACATTC
    GCAGCAGCCA TGTACATCCT GGGGGCCATC GAGATCTTGC TGACCTACAT TGCCCCACCA
    GCTGCCATTT TTTACCCATC GGGTGCTCAT GACACGTCGA ATGCCACTTT GAACAATATG
    CGTGTGTATG GGACCATTTT CCTGACCTTC ATGACCCTGG TGGTGTTTGT GGGGGTCAAG
    TATGTGAACA AATTTGCCTC GCTCTTCCTG GCCTGTGTGA TCATCTCCAT CCTCTCCATC
    TATGCTGGGG GCATAAAGTC TATATTTGAC CCTCCCGTGT TTCCGGTATG CATGCTGGGC
    AACAGGACCC TGTCCCGGGA CCAGTTTGAC ATCTGTGCCA AGACAGCTGT AGTGGACAAT
    GAGACAGTGG CCACCCAGCT ATGGAGTTTC TTCTGCCACA GCCCCAACCT TACGACCGAC
    TCCTGTGACC CCTACTTCAT GCTCAACAAT GTGACCGAGA TCCCTGGCAT CCCCGGGGCA
    GCTGCTGGTG TGCTCCAGGA AAACCTGTGG AGCGCCTACC TGGAGAAGGG TGACATCGTG
    GAGAAGCATG GGCTGCCCTC CGCAGATGCC CCGAGCCTGA AGGAGAGCCT GCCTCTGTAC
    GTGGTCGCTG ACATCGCCAC ATCCTTCACC GTGCTGGTCG GCATCTTCTT CCCTTCTGTA
    ACAGGCATCA TGGCTGGCTC AAACCGCTCT GGGGACCTTC GTGACGCCCA GAAGTCTATC
    CCTGTGGGGA CCATTCTGGC CATCATTACA ACTTCCCTCG TGTACTTCAG CAGTGTGGTT
    CTCTTTGGTG CCTGCATTGA GGGTGTGGTT CTCCGGGACA AGTATGGCGA TGGTGTCAGC
    AGGAACTTGG TGGTGGGCAC ACTGGCCTGG CCTTCACCCT GGGTCATCGT CATCGGCTCC
    TTCTTTTCAA CGTGTGGCGC TGGCCTCCAG AGCCTCACAG GGGCACCACG CCTATTGCAG
    GCCATTGCCA AGGACAACAT CATCCCCTTC CTCCGGGTGT TTGGCCACGG GAAGGTGAAT
    GGTGAACCCA CATGGGCACT CCTCCTGACG GCACTCATCG CCGAGCTGGG CATCCTCATC
    GCCTCCCTCG ACATGGTGGC CCCCATCTTA TCCATGTTCT TTCTGATGTG CTACCTGTTC
    GTGAACCTCG CCTGTGCGGT GCAGACACTC CTGAGGACCC CCAACTGGCG GCCCCGGTTC
    AAGTACTATC ACTGGGCGCT GTCCTTCCTG GGCATGAGTC TCTGCCTGGC CCTTATGTTT
    GTCTCCTCCT GGTACTATGC CCTGGTGGCC ATGCTCATCG CCGGCATGAT CTACAAATAC
    ATCGAGTACC AAGGGGCTGA GAAGGAGTGG GGTGACGGGA TCCGAGGCCT GTCCCTGAGC
    GCTGCCCGCT ACGCGCTGTT GCGGCTGGAG GAGGGGCCTC CTCACACCAA GAACTGGCGG
    CCGCAGCTGC TGGTGCTGCT GAAGCTGGAC GAGGACCTCC ACGTGAAGTA CCCGCGGCTC
    CTCACCTTCG CCTCCCAGCT CAAGGCTGGC AAGGGCCTGA CCATTGTTGG TTCTGTCATC
    CAGGGGAGCT TCTTGGAGAG CTATGGCGAG GCTCAGGCCG CCGAGCAGAC CATCAAGAAC
    ATGATGGAAA TTGAGAAGGT GAAGGGCTTC TGCCAGGTGG TGGTGGCCAG CAAGGTGCGG
    GAGGGGCTGG CCCACCTCAT CCAGTCCTGT GGCCTGGGAG GCATGCGGCA TAACTCCGTG
    GTGCTGGGCT GGCCCTACGG CTGGCGACAG AGCGAGGACC CCCGTGCCTG GAAGACCTTC
    ATTGACACCG TGCGCTGCAC TACGGCTGCC CACCTGGCCC TGCTCGTGCC CAAGAACATC
    GCCTTCTACC CCAGCAACCA CGAGCGCTAC CTGGAGGGCC ACATAGACGT GTGGTGGATC
    GTGCACGATG GTGGCATGCT CATGCTTCTG CCCTTCCTGC TGCGCCAGCA TAAGGTCTGG
    AGGAAGTGCC GGATGCGCAT CTTCACAGTG GCCCAGATGG ATGACAACAG CATCCAGATG
    AAGAAGGACC TGGCTGTCTT TCTGTACCAT CTGCGCCTTG AGGCCGAGGT GGAGGTGGTG
    GAGATGCATA ACAGTGACAT CTCTGCATAC ACCTACGAGC GGACGCTGAT GATGGAGCAG
    CGGTCGCAGA TGCTGCGGCA GATGAGACTG ACCAAGACTG AGCGGGAGCG AGAAGCCCAG
    CTGGTCAAGG ATCGGCACTC GGCCCTGCGG CTGGAGAGCC TGTACTCGGA CGAGGAAGAT
    GAGTCTGCAG TGGGGGCTGA CAAGATCCAG ATGACGTGGA CCAGGGACAA GTACATGACT
    GAGACCTGGG ACCCCAGCCA TGCCCCTGAC AATTTCCGGG AGCTGGTGCA CATTAAGCCG
    GACCAATCCA ATGTGCGGCG CATGCACACT GCTGTGAAGC TCAATGAAGT CATTGTCACG
    CGCTCCCACG ACGCCCGCCT GGTTCTCCTA AACATGCCTG GCCCACCCAG GAACAGTGAG
    GGCGACGAGA ACTACATGGA GTTCCTCGAG GTGCTGACCG AGGGCCTTGA GCGGGTGCTG
    TTGGTGCGCG GTGGTGGCCG TGAAGTCATC ACCATCTACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139436.1
    CDS105..3269
    Translation

    Target ORF information:

    RefSeq Version NM_001145964.1
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGGGACA CCCTGAGCCC CGGACATGGC AACCACAGAG AGAGCAGCCC TTTTCTTTCC 
    CCCTTGGAGG CTTCCAGAGG AATTGACTAC TATGACAGGA ACCTGGCACT GTTTGAGGAA
    GAGCTGGACA TCCGCCCAAA GGTATCGTCT CTTCTGGGAA AGCTCGTCAG CTACACCAAC
    CTCACCCAGG GCGCCAAAGA GCATGAGGAG GCCGAGAGTG GGGAGGGCAC CCGCCGGAGG
    GCAGCCGAGG CACCCAGCAT GGGCACCCTC ATGGGGGTGT ACCTGCCCTG CCTGCAGAAT
    ATCTTTGGGG TTATCCTCTT CCTGCGGCTG ACCTGGATGG TGGGCACAGC AGGTGTGCTA
    CAGGCCCTCC TCATCGTGCT TATCTGCTGC TGTTGTACCC TGCTGACGGC CATCTCCATG
    AGTGCCATCG CCACCAACGG TGTGGTTCCA GCTGGGGGCT CCTATTTCAT GATCTCTCGT
    TCACTGGGGC CAGAATTTGG AGGTGCTGTG GGCCTGTGCT TCTACCTGGG AACAACATTC
    GCAGCAGCCA TGTACATCCT GGGGGCCATC GAGATCTTGC TGACCTACAT TGCCCCACCA
    GCTGCCATTT TTTACCCATC GGGTGCTCAT GACACGTCGA ATGCCACTTT GAACAATATG
    CGTGTGTATG GGACCATTTT CCTGACCTTC ATGACCCTGG TGGTGTTTGT GGGGGTCAAG
    TATGTGAACA AATTTGCCTC GCTCTTCCTG GCCTGTGTGA TCATCTCCAT CCTCTCCATC
    TATGCTGGGG GCATAAAGTC TATATTTGAC CCTCCCGTGT TTCCGGTATG CATGCTGGGC
    AACAGGACCC TGTCCCGGGA CCAGTTTGAC ATCTGTGCCA AGACAGCTGT AGTGGACAAT
    GAGACAGTGG CCACCCAGCT ATGGAGTTTC TTCTGCCACA GCCCCAACCT TACGACCGAC
    TCCTGTGACC CCTACTTCAT GCTCAACAAT GTGACCGAGA TCCCTGGCAT CCCCGGGGCA
    GCTGCTGGTG TGCTCCAGGA AAACCTGTGG AGCGCCTACC TGGAGAAGGG TGACATCGTG
    GAGAAGCATG GGCTGCCCTC CGCAGATGCC CCGAGCCTGA AGGAGAGCCT GCCTCTGTAC
    GTGGTCGCTG ACATCGCCAC ATCCTTCACC GTGCTGGTCG GCATCTTCTT CCCTTCTGTA
    ACAGGCATCA TGGCTGGCTC AAACCGCTCT GGGGACCTTC GTGACGCCCA GAAGTCTATC
    CCTGTGGGGA CCATTCTGGC CATCATTACA ACTTCCCTCG TGTACTTCAG CAGTGTGGTT
    CTCTTTGGTG CCTGCATTGA GGGTGTGGTT CTCCGGGACA AGTATGGCGA TGGTGTCAGC
    AGGAACTTGG TGGTGGGCAC ACTGGCCTGG CCTTCACCCT GGGTCATCGT CATCGGCTCC
    TTCTTTTCAA CGTGTGGCGC TGGCCTCCAG AGCCTCACAG GGGCACCACG CCTATTGCAG
    GCCATTGCCA AGGACAACAT CATCCCCTTC CTCCGGGTGT TTGGCCACGG GAAGGTGAAT
    GGTGAACCCA CATGGGCACT CCTCCTGACG GCACTCATCG CCGAGCTGGG CATCCTCATC
    GCCTCCCTCG ACATGGTGGC CCCCATCTTA TCCATGTTCT TTCTGATGTG CTACCTGTTC
    GTGAACCTCG CCTGTGCGGT GCAGACACTC CTGAGGACCC CCAACTGGCG GCCCCGGTTC
    AAGTACTATC ACTGGGCGCT GTCCTTCCTG GGCATGAGTC TCTGCCTGGC CCTTATGTTT
    GTCTCCTCCT GGTACTATGC CCTGGTGGCC ATGCTCATCG CCGGCATGAT CTACAAATAC
    ATCGAGTACC AAGGGGCTGA GAAGGAGTGG GGTGACGGGA TCCGAGGCCT GTCCCTGAGC
    GCTGCCCGCT ACGCGCTGTT GCGGCTGGAG GAGGGGCCTC CTCACACCAA GAACTGGCGG
    CCGCAGCTGC TGGTGCTGCT GAAGCTGGAC GAGGACCTCC ACGTGAAGTA CCCGCGGCTC
    CTCACCTTCG CCTCCCAGCT CAAGGCTGGC AAGGGCCTGA CCATTGTTGG TTCTGTCATC
    CAGGGGAGCT TCTTGGAGAG CTATGGCGAG GCTCAGGCCG CCGAGCAGAC CATCAAGAAC
    ATGATGGAAA TTGAGAAGGT GAAGGGCTTC TGCCAGGTGG TGGTGGCCAG CAAGGTGCGG
    GAGGGGCTGG CCCACCTCAT CCAGTCCTGT GGCCTGGGAG GCATGCGGCA TAACTCCGTG
    GTGCTGGGCT GGCCCTACGG CTGGCGACAG AGCGAGGACC CCCGTGCCTG GAAGACCTTC
    ATTGACACCG TGCGCTGCAC TACGGCTGCC CACCTGGCCC TGCTCGTGCC CAAGAACATC
    GCCTTCTACC CCAGCAACCA CGAGCGCTAC CTGGAGGGCC ACATAGACGT GTGGTGGATC
    GTGCACGATG GTGGCATGCT CATGCTTCTG CCCTTCCTGC TGCGCCAGCA TAAGGTCTGG
    AGGAAGTGCC GGATGCGCAT CTTCACAGTG GCCCAGATGG ATGACAACAG CATCCAGATG
    AAGAAGGACC TGGCTGTCTT TCTGTACCAT CTGCGCCTTG AGGCCGAGGT GGAGGTGGTG
    GAGATGCATA ACAGTGACAT CTCTGCATAC ACCTACGAGC GGACGCTGAT GATGGAGCAG
    CGGTCGCAGA TGCTGCGGCA GATGAGACTG ACCAAGACTG AGCGGGAGCG AGAAGCCCAG
    CTGGTCAAGG ATCGGCACTC GGCCCTGCGG CTGGAGAGCC TGTACTCGGA CGAGGAAGAT
    GAGTCTGCAG TGGGGGCTGA CAAGATCCAG ATGACGTGGA CCAGGGACAA GTACATGACT
    GAGACCTGGG ACCCCAGCCA TGCCCCTGAC AATTTCCGGG AGCTGGTGCA CATTAAGCCG
    GACCAATCCA ATGTGCGGCG CATGCACACT GCTGTGAAGC TCAATGAAGT CATTGTCACG
    CGCTCCCACG ACGCCCGCCT GGTTCTCCTA AACATGCCTG GCCCACCCAG GAACAGTGAG
    GGCGACGAGA ACTACATGGA GTTCCTCGAG GTGCTGACCG AGGGCCTTGA GCGGGTGCTG
    TTGGTGCGCG GTGGTGGCCG TGAAGTCATC ACCATCTACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09216
    Clone ID Related Accession (Same CDS sequence) NM_001145961.1 , NM_001145961.2
    Accession Version NM_001145961.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321966.1, AK293906.1, BC021193.2, CF272417.1, AK310027.1 and AI368074.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293906.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139433.1
    CDS142..3381
    Translation

    Target ORF information:

    RefSeq Version NM_001145961.1
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145961.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09216
    Clone ID Related Accession (Same CDS sequence) NM_001145961.1 , NM_001145961.2
    Accession Version NM_001145961.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321966.1, AK293906.1, BC021193.2, CF272417.1, AK310027.1 and AI368074.1. On May 31, 2019 this sequence version replaced NM_001145961.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293906.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139433.1
    CDS85..3324
    Translation

    Target ORF information:

    RefSeq Version NM_001145961.2
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145961.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GCATAACAGT GACATCTCTG CATACACCTA CGAGCGGACG
    CTGATGATGG AGCAGCGGTC GCAGATGCTG CGGCAGATGA GACTGACCAA GACTGAGCGG
    GAGCGAGAAG CCCAGCTGGT CAAGGATCGG CACTCGGCCC TGCGGCTGGA GAGCCTGTAC
    TCGGACGAGG AAGATGAGTC TGCAGTGGGG GCTGACAAGA TCCAGATGAC GTGGACCAGG
    GACAAGTACA TGACTGAGAC CTGGGACCCC AGCCATGCCC CTGACAATTT CCGGGAGCTG
    GTGCACATTA AGCCGGACCA ATCCAATGTG CGGCGCATGC ACACTGCTGT GAAGCTCAAT
    GAAGTCATTG TCACGCGCTC CCACGACGCC CGCCTGGTTC TCCTAAACAT GCCTGGCCCA
    CCCAGGAACA GTGAGGGCGA CGAGAACTAC ATGGAGTTCC TCGAGGTGCT GACCGAGGGC
    CTTGAGCGGG TGCTGTTGGT GCGCGGTGGT GGCCGTGAAG TCATCACCAT CTACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17960
    Clone ID Related Accession (Same CDS sequence) NM_005072.5 , NM_005072.4
    Accession Version NM_005072.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321966.1, AF047338.1, CF272417.1, AK310027.1 and AI368074.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005072.4. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021193.2, SRR1803614.234883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000316341.8/ ENSP00000318557.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GGTGGAGGTG GTGGAGATGC ATAACAGTGA CATCTCTGCA
    TACACCTACG AGCGGACGCT GATGATGGAG CAGCGGTCGC AGATGCTGCG GCAGATGAGA
    CTGACCAAGA CTGAGCGGGA GCGAGAAGCC CAGCTGGTCA AGGATCGGCA CTCGGCCCTG
    CGGCTGGAGA GCCTGTACTC GGACGAGGAA GATGAGTCTG CAGTGGGGGC TGACAAGATC
    CAGATGACGT GGACCAGGGA CAAGTACATG ACTGAGACCT GGGACCCCAG CCATGCCCCT
    GACAATTTCC GGGAGCTGGT GCACATTAAG CCGGACCAAT CCAATGTGCG GCGCATGCAC
    ACTGCTGTGA AGCTCAATGA AGTCATTGTC ACGCGCTCCC ACGACGCCCG CCTGGTTCTC
    CTAAACATGC CTGGCCCACC CAGGAACAGT GAGGGCGACG AGAACTACAT GGAGTTCCTC
    GAGGTGCTGA CCGAGGGCCT TGAGCGGGTG CTGTTGGTGC GCGGTGGTGG CCGTGAAGTC
    ATCACCATCT ACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005063.1
    CDS85..3342
    Translation

    Target ORF information:

    RefSeq Version NM_005072.5
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005072.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GGTGGAGGTG GTGGAGATGC ATAACAGTGA CATCTCTGCA
    TACACCTACG AGCGGACGCT GATGATGGAG CAGCGGTCGC AGATGCTGCG GCAGATGAGA
    CTGACCAAGA CTGAGCGGGA GCGAGAAGCC CAGCTGGTCA AGGATCGGCA CTCGGCCCTG
    CGGCTGGAGA GCCTGTACTC GGACGAGGAA GATGAGTCTG CAGTGGGGGC TGACAAGATC
    CAGATGACGT GGACCAGGGA CAAGTACATG ACTGAGACCT GGGACCCCAG CCATGCCCCT
    GACAATTTCC GGGAGCTGGT GCACATTAAG CCGGACCAAT CCAATGTGCG GCGCATGCAC
    ACTGCTGTGA AGCTCAATGA AGTCATTGTC ACGCGCTCCC ACGACGCCCG CCTGGTTCTC
    CTAAACATGC CTGGCCCACC CAGGAACAGT GAGGGCGACG AGAACTACAT GGAGTTCCTC
    GAGGTGCTGA CCGAGGGCCT TGAGCGGGTG CTGTTGGTGC GCGGTGGTGG CCGTGAAGTC
    ATCACCATCT ACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17960
    Clone ID Related Accession (Same CDS sequence) NM_005072.5 , NM_005072.4
    Accession Version NM_005072.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321966.1, AF047338.1, CF272417.1, AK310027.1 and AI368074.1. This sequence is a reference standard in the RefSeqGene project. On Mar 24, 2009 this sequence version replaced NM_005072.3. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021193.2, SRR1803614.234883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GGTGGAGGTG GTGGAGATGC ATAACAGTGA CATCTCTGCA
    TACACCTACG AGCGGACGCT GATGATGGAG CAGCGGTCGC AGATGCTGCG GCAGATGAGA
    CTGACCAAGA CTGAGCGGGA GCGAGAAGCC CAGCTGGTCA AGGATCGGCA CTCGGCCCTG
    CGGCTGGAGA GCCTGTACTC GGACGAGGAA GATGAGTCTG CAGTGGGGGC TGACAAGATC
    CAGATGACGT GGACCAGGGA CAAGTACATG ACTGAGACCT GGGACCCCAG CCATGCCCCT
    GACAATTTCC GGGAGCTGGT GCACATTAAG CCGGACCAAT CCAATGTGCG GCGCATGCAC
    ACTGCTGTGA AGCTCAATGA AGTCATTGTC ACGCGCTCCC ACGACGCCCG CCTGGTTCTC
    CTAAACATGC CTGGCCCACC CAGGAACAGT GAGGGCGACG AGAACTACAT GGAGTTCCTC
    GAGGTGCTGA CCGAGGGCCT TGAGCGGGTG CTGTTGGTGC GCGGTGGTGG CCGTGAAGTC
    ATCACCATCT ACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005063.1
    CDS142..3399
    Translation

    Target ORF information:

    RefSeq Version NM_005072.4
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005072.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCACT TCACCGTGGT GCCAGTGGAC GGGCCGAGGC GCGGCGACTA TGACAACCTC 
    GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC TGGATGACTC GGACGGACAT
    GGCAACCACA GAGAGAGCAG CCCTTTTCTT TCCCCCTTGG AGGCTTCCAG AGGAATTGAC
    TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTATCG
    TCTCTTCTGG GAAAGCTCGT CAGCTACACC AACCTCACCC AGGGCGCCAA AGAGCATGAG
    GAGGCCGAGA GTGGGGAGGG CACCCGCCGG AGGGCAGCCG AGGCACCCAG CATGGGCACC
    CTCATGGGGG TGTACCTGCC CTGCCTGCAG AATATCTTTG GGGTTATCCT CTTCCTGCGG
    CTGACCTGGA TGGTGGGCAC AGCAGGTGTG CTACAGGCCC TCCTCATCGT GCTTATCTGC
    TGCTGTTGTA CCCTGCTGAC GGCCATCTCC ATGAGTGCCA TCGCCACCAA CGGTGTGGTT
    CCAGCTGGGG GCTCCTATTT CATGATCTCT CGTTCACTGG GGCCAGAATT TGGAGGTGCT
    GTGGGCCTGT GCTTCTACCT GGGAACAACA TTCGCAGCAG CCATGTACAT CCTGGGGGCC
    ATCGAGATCT TGCTGACCTA CATTGCCCCA CCAGCTGCCA TTTTTTACCC ATCGGGTGCT
    CATGACACGT CGAATGCCAC TTTGAACAAT ATGCGTGTGT ATGGGACCAT TTTCCTGACC
    TTCATGACCC TGGTGGTGTT TGTGGGGGTC AAGTATGTGA ACAAATTTGC CTCGCTCTTC
    CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATAAA GTCTATATTT
    GACCCTCCCG TGTTTCCGGT ATGCATGCTG GGCAACAGGA CCCTGTCCCG GGACCAGTTT
    GACATCTGTG CCAAGACAGC TGTAGTGGAC AATGAGACAG TGGCCACCCA GCTATGGAGT
    TTCTTCTGCC ACAGCCCCAA CCTTACGACC GACTCCTGTG ACCCCTACTT CATGCTCAAC
    AATGTGACCG AGATCCCTGG CATCCCCGGG GCAGCTGCTG GTGTGCTCCA GGAAAACCTG
    TGGAGCGCCT ACCTGGAGAA GGGTGACATC GTGGAGAAGC ATGGGCTGCC CTCCGCAGAT
    GCCCCGAGCC TGAAGGAGAG CCTGCCTCTG TACGTGGTCG CTGACATCGC CACATCCTTC
    ACCGTGCTGG TCGGCATCTT CTTCCCTTCT GTAACAGGCA TCATGGCTGG CTCAAACCGC
    TCTGGGGACC TTCGTGACGC CCAGAAGTCT ATCCCTGTGG GGACCATTCT GGCCATCATT
    ACAACTTCCC TCGTGTACTT CAGCAGTGTG GTTCTCTTTG GTGCCTGCAT TGAGGGTGTG
    GTTCTCCGGG ACAAGTATGG CGATGGTGTC AGCAGGAACT TGGTGGTGGG CACACTGGCC
    TGGCCTTCAC CCTGGGTCAT CGTCATCGGC TCCTTCTTTT CAACGTGTGG CGCTGGCCTC
    CAGAGCCTCA CAGGGGCACC ACGCCTATTG CAGGCCATTG CCAAGGACAA CATCATCCCC
    TTCCTCCGGG TGTTTGGCCA CGGGAAGGTG AATGGTGAAC CCACATGGGC ACTCCTCCTG
    ACGGCACTCA TCGCCGAGCT GGGCATCCTC ATCGCCTCCC TCGACATGGT GGCCCCCATC
    TTATCCATGT TCTTTCTGAT GTGCTACCTG TTCGTGAACC TCGCCTGTGC GGTGCAGACA
    CTCCTGAGGA CCCCCAACTG GCGGCCCCGG TTCAAGTACT ATCACTGGGC GCTGTCCTTC
    CTGGGCATGA GTCTCTGCCT GGCCCTTATG TTTGTCTCCT CCTGGTACTA TGCCCTGGTG
    GCCATGCTCA TCGCCGGCAT GATCTACAAA TACATCGAGT ACCAAGGGGC TGAGAAGGAG
    TGGGGTGACG GGATCCGAGG CCTGTCCCTG AGCGCTGCCC GCTACGCGCT GTTGCGGCTG
    GAGGAGGGGC CTCCTCACAC CAAGAACTGG CGGCCGCAGC TGCTGGTGCT GCTGAAGCTG
    GACGAGGACC TCCACGTGAA GTACCCGCGG CTCCTCACCT TCGCCTCCCA GCTCAAGGCT
    GGCAAGGGCC TGACCATTGT TGGTTCTGTC ATCCAGGGGA GCTTCTTGGA GAGCTATGGC
    GAGGCTCAGG CCGCCGAGCA GACCATCAAG AACATGATGG AAATTGAGAA GGTGAAGGGC
    TTCTGCCAGG TGGTGGTGGC CAGCAAGGTG CGGGAGGGGC TGGCCCACCT CATCCAGTCC
    TGTGGCCTGG GAGGCATGCG GCATAACTCC GTGGTGCTGG GCTGGCCCTA CGGCTGGCGA
    CAGAGCGAGG ACCCCCGTGC CTGGAAGACC TTCATTGACA CCGTGCGCTG CACTACGGCT
    GCCCACCTGG CCCTGCTCGT GCCCAAGAAC ATCGCCTTCT ACCCCAGCAA CCACGAGCGC
    TACCTGGAGG GCCACATAGA CGTGTGGTGG ATCGTGCACG ATGGTGGCAT GCTCATGCTT
    CTGCCCTTCC TGCTGCGCCA GCATAAGGTC TGGAGGAAGT GCCGGATGCG CATCTTCACA
    GTGGCCCAGA TGGATGACAA CAGCATCCAG ATGAAGAAGG ACCTGGCTGT CTTTCTGTAC
    CATCTGCGCC TTGAGGCCGA GGTGGAGGTG GTGGAGATGC ATAACAGTGA CATCTCTGCA
    TACACCTACG AGCGGACGCT GATGATGGAG CAGCGGTCGC AGATGCTGCG GCAGATGAGA
    CTGACCAAGA CTGAGCGGGA GCGAGAAGCC CAGCTGGTCA AGGATCGGCA CTCGGCCCTG
    CGGCTGGAGA GCCTGTACTC GGACGAGGAA GATGAGTCTG CAGTGGGGGC TGACAAGATC
    CAGATGACGT GGACCAGGGA CAAGTACATG ACTGAGACCT GGGACCCCAG CCATGCCCCT
    GACAATTTCC GGGAGCTGGT GCACATTAAG CCGGACCAAT CCAATGTGCG GCGCATGCAC
    ACTGCTGTGA AGCTCAATGA AGTCATTGTC ACGCGCTCCC ACGACGCCCG CCTGGTTCTC
    CTAAACATGC CTGGCCCACC CAGGAACAGT GAGGGCGACG AGAACTACAT GGAGTTCCTC
    GAGGTGCTGA CCGAGGGCCT TGAGCGGGTG CTGTTGGTGC GCGGTGGTGG CCGTGAAGTC
    ATCACCATCT ACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09056
    Clone ID Related Accession (Same CDS sequence) NM_001145962.1
    Accession Version NM_001145962.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-07
    Organism Homo sapiens(human)
    Product solute carrier family 12 member 4 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321966.1, AK293956.1, AF047338.1, CF272417.1, AK310027.1 and AI368074.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.238528.1, AK293956.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGAGCGG GCGGGGCGTG TCGGCCCGGG GCGGCGGGGA CAGCGGCGGG GACAGCGGCG 
    GGCGGCTGGG ACGGCGGGTG CGGCGGGGCC GAGCCCGCAC GATGCCTCAC TTCACCGTGG
    TGCCAGTGGA CGGGCCGAGG CGCGGCGACT ATGACAACCT CGAGGGGCTC AGTTGGGTGG
    ACTACGGGGA GCGCGCCGAG CTGGATGACT CGGACGGAAG AGCTGGACAT CCGCCCAAAG
    GTATCGTCTC TTCTGGGAAA GCTCGTCAGC TACACCAACC TCACCCAGGG CGCCAAAGAG
    CATGAGGAGG CCGAGAGTGG GGAGGGCACC CGCCGGAGGG CAGCCGAGGC ACCCAGCATG
    GGCACCCTCA TGGGGGTGTA CCTGCCCTGC CTGCAGAATA TCTTTGGGGT TATCCTCTTC
    CTGCGGCTGA CCTGGATGGT GGGCACAGCA GGTGTGCTAC AGGCCCTCCT CATCGTGCTT
    ATCTGCTGCT GTTGTACCCT GCTGACGGCC ATCTCCATGA GTGCCATCGC CACCAACGGT
    GTGGTTCCAG CTGGGGGCTC CTATTTCATG ATCTCTCGTT CACTGGGGCC AGAATTTGGA
    GGTGCTGTGG GCCTGTGCTT CTACCTGGGA ACAACATTCG CAGCAGCCAT GTACATCCTG
    GGGGCCATCG AGATCTTGCT GACCTACATT GCCCCACCAG CTGCCATTTT TTACCCATCG
    GGTGCTCATG ACACGTCGAA TGCCACTTTG AACAATATGC GTGTGTATGG GACCATTTTC
    CTGACCTTCA TGACCCTGGT GGTGTTTGTG GGGGTCAAGT ATGTGAACAA ATTTGCCTCG
    CTCTTCCTGG CCTGTGTGAT CATCTCCATC CTCTCCATCT ATGCTGGGGG CATAAAGTCT
    ATATTTGACC CTCCCGTGTT TCCGGTATGC ATGCTGGGCA ACAGGACCCT GTCCCGGGAC
    CAGTTTGACA TCTGTGCCAA GACAGCTGTA GTGGACAATG AGACAGTGGC CACCCAGCTA
    TGGAGTTTCT TCTGCCACAG CCCCAACCTT ACGACCGACT CCTGTGACCC CTACTTCATG
    CTCAACAATG TGACCGAGAT CCCTGGCATC CCCGGGGCAG CTGCTGGTGT GCTCCAGGAA
    AACCTGTGGA GCGCCTACCT GGAGAAGGGT GACATCGTGG AGAAGCATGG GCTGCCCTCC
    GCAGATGCCC CGAGCCTGAA GGAGAGCCTG CCTCTGTACG TGGTCGCTGA CATCGCCACA
    TCCTTCACCG TGCTGGTCGG CATCTTCTTC CCTTCTGTAA CAGGCATCAT GGCTGGCTCA
    AACCGCTCTG GGGACCTTCG TGACGCCCAG AAGTCTATCC CTGTGGGGAC CATTCTGGCC
    ATCATTACAA CTTCCCTCGT GTACTTCAGC AGTGTGGTTC TCTTTGGTGC CTGCATTGAG
    GGTGTGGTTC TCCGGGACAA GTATGGCGAT GGTGTCAGCA GGAACTTGGT GGTGGGCACA
    CTGGCCTGGC CTTCACCCTG GGTCATCGTC ATCGGCTCCT TCTTTTCAAC GTGTGGCGCT
    GGCCTCCAGA GCCTCACAGG GGCACCACGC CTATTGCAGG CCATTGCCAA GGACAACATC
    ATCCCCTTCC TCCGGGTGTT TGGCCACGGG AAGGTGAATG GTGAACCCAC ATGGGCACTC
    CTCCTGACGG CACTCATCGC CGAGCTGGGC ATCCTCATCG CCTCCCTCGA CATGGTGGCC
    CCCATCTTAT CCATGTTCTT TCTGATGTGC TACCTGTTCG TGAACCTCGC CTGTGCGGTG
    CAGACACTCC TGAGGACCCC CAACTGGCGG CCCCGGTTCA AGTACTATCA CTGGGCGCTG
    TCCTTCCTGG GCATGAGTCT CTGCCTGGCC CTTATGTTTG TCTCCTCCTG GTACTATGCC
    CTGGTGGCCA TGCTCATCGC CGGCATGATC TACAAATACA TCGAGTACCA AGGGGCTGAG
    AAGGAGTGGG GTGACGGGAT CCGAGGCCTG TCCCTGAGCG CTGCCCGCTA CGCGCTGTTG
    CGGCTGGAGG AGGGGCCTCC TCACACCAAG AACTGGCGGC CGCAGCTGCT GGTGCTGCTG
    AAGCTGGACG AGGACCTCCA CGTGAAGTAC CCGCGGCTCC TCACCTTCGC CTCCCAGCTC
    AAGGCTGGCA AGGGCCTGAC CATTGTTGGT TCTGTCATCC AGGGGAGCTT CTTGGAGAGC
    TATGGCGAGG CTCAGGCCGC CGAGCAGACC ATCAAGAACA TGATGGAAAT TGAGAAGGTG
    AAGGGCTTCT GCCAGGTGGT GGTGGCCAGC AAGGTGCGGG AGGGGCTGGC CCACCTCATC
    CAGTCCTGTG GCCTGGGAGG CATGCGGCAT AACTCCGTGG TGCTGGGCTG GCCCTACGGC
    TGGCGACAGA GCGAGGACCC CCGTGCCTGG AAGACCTTCA TTGACACCGT GCGCTGCACT
    ACGGCTGCCC ACCTGGCCCT GCTCGTGCCC AAGAACATCG CCTTCTACCC CAGCAACCAC
    GAGCGCTACC TGGAGGGCCA CATAGACGTG TGGTGGATCG TGCACGATGG TGGCATGCTC
    ATGCTTCTGC CCTTCCTGCT GCGCCAGCAT AAGGTCTGGA GGAAGTGCCG GATGCGCATC
    TTCACAGTGG CCCAGATGGA TGACAACAGC ATCCAGATGA AGAAGGACCT GGCTGTCTTT
    CTGTACCATC TGCGCCTTGA GGCCGAGGTG GAGGTGGTGG AGATGCATAA CAGTGACATC
    TCTGCATACA CCTACGAGCG GACGCTGATG ATGGAGCAGC GGTCGCAGAT GCTGCGGCAG
    ATGAGACTGA CCAAGACTGA GCGGGAGCGA GAAGCCCAGC TGGTCAAGGA TCGGCACTCG
    GCCCTGCGGC TGGAGAGCCT GTACTCGGAC GAGGAAGATG AGTCTGCAGT GGGGGCTGAC
    AAGATCCAGA TGACGTGGAC CAGGGACAAG TACATGACTG AGACCTGGGA CCCCAGCCAT
    GCCCCTGACA ATTTCCGGGA GCTGGTGCAC ATTAAGCCGG ACCAATCCAA TGTGCGGCGC
    ATGCACACTG CTGTGAAGCT CAATGAAGTC ATTGTCACGC GCTCCCACGA CGCCCGCCTG
    GTTCTCCTAA ACATGCCTGG CCCACCCAGG AACAGTGAGG GCGACGAGAA CTACATGGAG
    TTCCTCGAGG TGCTGACCGA GGGCCTTGAG CGGGTGCTGT TGGTGCGCGG TGGTGGCCGT
    GAAGTCATCA CCATCTACTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139434.1
    CDS41..3304
    Translation

    Target ORF information:

    RefSeq Version NM_001145962.1
    Organism Homo sapiens(human)
    Definition Homo sapiens solute carrier family 12 member 4 (SLC12A4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145962.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGAGCGG GCGGGGCGTG TCGGCCCGGG GCGGCGGGGA CAGCGGCGGG GACAGCGGCG 
    GGCGGCTGGG ACGGCGGGTG CGGCGGGGCC GAGCCCGCAC GATGCCTCAC TTCACCGTGG
    TGCCAGTGGA CGGGCCGAGG CGCGGCGACT ATGACAACCT CGAGGGGCTC AGTTGGGTGG
    ACTACGGGGA GCGCGCCGAG CTGGATGACT CGGACGGAAG AGCTGGACAT CCGCCCAAAG
    GTATCGTCTC TTCTGGGAAA GCTCGTCAGC TACACCAACC TCACCCAGGG CGCCAAAGAG
    CATGAGGAGG CCGAGAGTGG GGAGGGCACC CGCCGGAGGG CAGCCGAGGC ACCCAGCATG
    GGCACCCTCA TGGGGGTGTA CCTGCCCTGC CTGCAGAATA TCTTTGGGGT TATCCTCTTC
    CTGCGGCTGA CCTGGATGGT GGGCACAGCA GGTGTGCTAC AGGCCCTCCT CATCGTGCTT
    ATCTGCTGCT GTTGTACCCT GCTGACGGCC ATCTCCATGA GTGCCATCGC CACCAACGGT
    GTGGTTCCAG CTGGGGGCTC CTATTTCATG ATCTCTCGTT CACTGGGGCC AGAATTTGGA
    GGTGCTGTGG GCCTGTGCTT CTACCTGGGA ACAACATTCG CAGCAGCCAT GTACATCCTG
    GGGGCCATCG AGATCTTGCT GACCTACATT GCCCCACCAG CTGCCATTTT TTACCCATCG
    GGTGCTCATG ACACGTCGAA TGCCACTTTG AACAATATGC GTGTGTATGG GACCATTTTC
    CTGACCTTCA TGACCCTGGT GGTGTTTGTG GGGGTCAAGT ATGTGAACAA ATTTGCCTCG
    CTCTTCCTGG CCTGTGTGAT CATCTCCATC CTCTCCATCT ATGCTGGGGG CATAAAGTCT
    ATATTTGACC CTCCCGTGTT TCCGGTATGC ATGCTGGGCA ACAGGACCCT GTCCCGGGAC
    CAGTTTGACA TCTGTGCCAA GACAGCTGTA GTGGACAATG AGACAGTGGC CACCCAGCTA
    TGGAGTTTCT TCTGCCACAG CCCCAACCTT ACGACCGACT CCTGTGACCC CTACTTCATG
    CTCAACAATG TGACCGAGAT CCCTGGCATC CCCGGGGCAG CTGCTGGTGT GCTCCAGGAA
    AACCTGTGGA GCGCCTACCT GGAGAAGGGT GACATCGTGG AGAAGCATGG GCTGCCCTCC
    GCAGATGCCC CGAGCCTGAA GGAGAGCCTG CCTCTGTACG TGGTCGCTGA CATCGCCACA
    TCCTTCACCG TGCTGGTCGG CATCTTCTTC CCTTCTGTAA CAGGCATCAT GGCTGGCTCA
    AACCGCTCTG GGGACCTTCG TGACGCCCAG AAGTCTATCC CTGTGGGGAC CATTCTGGCC
    ATCATTACAA CTTCCCTCGT GTACTTCAGC AGTGTGGTTC TCTTTGGTGC CTGCATTGAG
    GGTGTGGTTC TCCGGGACAA GTATGGCGAT GGTGTCAGCA GGAACTTGGT GGTGGGCACA
    CTGGCCTGGC CTTCACCCTG GGTCATCGTC ATCGGCTCCT TCTTTTCAAC GTGTGGCGCT
    GGCCTCCAGA GCCTCACAGG GGCACCACGC CTATTGCAGG CCATTGCCAA GGACAACATC
    ATCCCCTTCC TCCGGGTGTT TGGCCACGGG AAGGTGAATG GTGAACCCAC ATGGGCACTC
    CTCCTGACGG CACTCATCGC CGAGCTGGGC ATCCTCATCG CCTCCCTCGA CATGGTGGCC
    CCCATCTTAT CCATGTTCTT TCTGATGTGC TACCTGTTCG TGAACCTCGC CTGTGCGGTG
    CAGACACTCC TGAGGACCCC CAACTGGCGG CCCCGGTTCA AGTACTATCA CTGGGCGCTG
    TCCTTCCTGG GCATGAGTCT CTGCCTGGCC CTTATGTTTG TCTCCTCCTG GTACTATGCC
    CTGGTGGCCA TGCTCATCGC CGGCATGATC TACAAATACA TCGAGTACCA AGGGGCTGAG
    AAGGAGTGGG GTGACGGGAT CCGAGGCCTG TCCCTGAGCG CTGCCCGCTA CGCGCTGTTG
    CGGCTGGAGG AGGGGCCTCC TCACACCAAG AACTGGCGGC CGCAGCTGCT GGTGCTGCTG
    AAGCTGGACG AGGACCTCCA CGTGAAGTAC CCGCGGCTCC TCACCTTCGC CTCCCAGCTC
    AAGGCTGGCA AGGGCCTGAC CATTGTTGGT TCTGTCATCC AGGGGAGCTT CTTGGAGAGC
    TATGGCGAGG CTCAGGCCGC CGAGCAGACC ATCAAGAACA TGATGGAAAT TGAGAAGGTG
    AAGGGCTTCT GCCAGGTGGT GGTGGCCAGC AAGGTGCGGG AGGGGCTGGC CCACCTCATC
    CAGTCCTGTG GCCTGGGAGG CATGCGGCAT AACTCCGTGG TGCTGGGCTG GCCCTACGGC
    TGGCGACAGA GCGAGGACCC CCGTGCCTGG AAGACCTTCA TTGACACCGT GCGCTGCACT
    ACGGCTGCCC ACCTGGCCCT GCTCGTGCCC AAGAACATCG CCTTCTACCC CAGCAACCAC
    GAGCGCTACC TGGAGGGCCA CATAGACGTG TGGTGGATCG TGCACGATGG TGGCATGCTC
    ATGCTTCTGC CCTTCCTGCT GCGCCAGCAT AAGGTCTGGA GGAAGTGCCG GATGCGCATC
    TTCACAGTGG CCCAGATGGA TGACAACAGC ATCCAGATGA AGAAGGACCT GGCTGTCTTT
    CTGTACCATC TGCGCCTTGA GGCCGAGGTG GAGGTGGTGG AGATGCATAA CAGTGACATC
    TCTGCATACA CCTACGAGCG GACGCTGATG ATGGAGCAGC GGTCGCAGAT GCTGCGGCAG
    ATGAGACTGA CCAAGACTGA GCGGGAGCGA GAAGCCCAGC TGGTCAAGGA TCGGCACTCG
    GCCCTGCGGC TGGAGAGCCT GTACTCGGAC GAGGAAGATG AGTCTGCAGT GGGGGCTGAC
    AAGATCCAGA TGACGTGGAC CAGGGACAAG TACATGACTG AGACCTGGGA CCCCAGCCAT
    GCCCCTGACA ATTTCCGGGA GCTGGTGCAC ATTAAGCCGG ACCAATCCAA TGTGCGGCGC
    ATGCACACTG CTGTGAAGCT CAATGAAGTC ATTGTCACGC GCTCCCACGA CGCCCGCCTG
    GTTCTCCTAA ACATGCCTGG CCCACCCAGG AACAGTGAGG GCGACGAGAA CTACATGGAG
    TTCCTCGAGG TGCTGACCGA GGGCCTTGAG CGGGTGCTGT TGGTGCGCGG TGGTGGCCGT
    GAAGTCATCA CCATCTACTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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