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CYP4F12 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol CYP4F12
Entrez Gene ID 66002
Full Name cytochrome P450 family 4 subfamily F member 12
Synonyms CYPIVF12,F22329_1
General protein information
Preferred Names

cytochrome P450 family 4 subfamily F member 12

Names

cytochrome P450 4F12
cytochrome P450, family 4, subfamily F, polypeptide 12
cytochrome P450, subfamily IVF, polypeptide 12
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19p13.12
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014].
Disorder MIM:

611485


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017027172.1 XP_016882661.1 cytochrome P450 4F12 isoform X1
XM_006722850.3 XP_006722913.1 cytochrome P450 4F12 isoform X2
XM_011528204.2 XP_011526506.1 cytochrome P450 4F12 isoform X3
XM_011528202.2 XP_011526504.1 cytochrome P450 4F12 isoform X2
XM_011528203.2 XP_011526505.1 cytochrome P450 4F12 isoform X3
XM_011528208.2 XP_011526510.1 cytochrome P450 4F12 isoform X2
XM_011528202.3 XP_011526504.1 cytochrome P450 4F12 isoform X2
XM_011528207.2 XP_011526509.1 cytochrome P450 4F12 isoform X4
NM_023944.3 NP_076433.3 cytochrome P450 4F12
XM_011528205.2 XP_011526507.1 cytochrome P450 4F12 isoform X3
NM_023944.4 NP_076433.3 cytochrome P450 4F12

Pathways from BioSystems


Homologies


CYP4F12 XP_001112408.1
Homo sapiens (human) CYP4F12 NP_076433.3
Pan troglodytes (chimpanzee) LOC468754 XP_524141.2
Rattus norvegicus (Norway rat) Cyp4f1 NP_062569.2
Oryza sativa (rice) Os02g0204700 NP_001172847.1
Mus musculus (house mouse) Cyp4f14 NP_001191262.1
Arabidopsis thaliana (thale cress) BAS1 NP_180239.1
Oryza sativa (rice) Os06g0600400 NP_001058000.1

Gene Ontology


Bibliography

Related articles in PubMed

Terfenadine t-butyl hydroxylation catalyzed by human and marmoset cytochrome P450 3A and 4F enzymes in livers and small intestines.
Uehara S, Yuki Y, Uno Y, Inoue T, Sasaki E, Yamazaki H
Xenobiotica; the fate of foreign compounds in biological systems1-6(2017 May)

Inducible CYP4F12 enhances Hepatitis C virus infection via association with viral nonstructural protein 5B.
Zhu SL, Wang L, Cao ZY, Wang J, Jing MZ, Xia ZC, Ao F, Ye LB, Liu S, Zhu Y
Biochemical and biophysical research communications471(1)95-102(2016 Feb)

Identification of pregnane-X receptor target genes and coactivator and corepressor binding to promoter elements in human hepatocytes.
Hariparsad N, Chu X, Yabut J, Labhart P, Hartley DP, Dai X, Evers R
Nucleic acids research37(4)1160-73(2009 Mar)

Human CYP4F12 genetic polymorphism: identification and functional characterization of seven variant allozymes.
Cauffiez C, Klinzig F, Rat E, Tournel G, Allorge D, Chevalier D, Pottier N, Lovecchio T, Colombel JF, Lhermitte M, Lo-Guidice JM, Broly F
Biochemical pharmacology68(12)2417-25(2004 Dec)

Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants.
Nelson DR, Zeldin DC, Hoffman SM, Maltais LJ, Wain HM, Nebert DW
Pharmacogenetics14(1)1-18(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results showed that HCV infection induced expression of CYP4F12 protein, which bound to the HCV replication complex to facilitate viral replication.
Title: Inducible CYP4F12 enhances Hepatitis C virus infection via association with viral nonstructural protein 5B.

3-hydroxystearate and 3-hydroxypalmitate are converted to omega-hydroxylated 3-OHDCA precursors in liver; CYP4F11 and, to a lesser extent, CYP4F2 catalyzed omega-hydroxylation of 3-hydroxystearate; CYP4F3b, CYP4F12, and CYP4A11 had negligible activity.
Title: Omega oxidation of 3-hydroxy fatty acids by the human CYP4F gene subfamily enzyme CYP4F11.

We conclude that CYP4F8 and CYP4F12 catalyze epoxidation of 22:6n-3 and 22:5n-3, and CYP4F8 omega3-hydroxylation of 22:5n-6.
Title: Oxygenation of polyunsaturated long chain fatty acids by recombinant CYP4F8 and CYP4F12 and catalytic importance of Tyr-125 and Gly-328 of CYP4F8.

The following CYP4F12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP4F12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21164 NM_023944.4
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu51770 XM_006722850.3
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu51770 XM_011528208.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu51770 XM_011528202.3
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu51771 XM_011528207.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71682 XM_011528203.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71682 XM_011528204.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71682 XM_011528205.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu88680 XM_017027172.1
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu51770 XM_011528202.2 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu21164 NM_023944.3 Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu21164
Clone ID Related Accession (Same CDS sequence) NM_023944.3 , NM_023944.4
Accession Version NM_023944.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-22
Organism Homo sapiens(human)
Product cytochrome P450 4F12
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122702.2, DC418592.1, AB035130.1 and DR422336.1. This sequence is a reference standard in the RefSeqGene project. On May 14, 2011 this sequence version replaced NM_023944.2. Transcript Variant: This variant (1) represents the shorter transcript but encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035130.1, BC035350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCGCTGC TGAGCCTGCC CTGGCTGGGC CTCAGACCGG TGGCAACGTC CCCATGGCTA 
CTCCTGCTGC TGGTTGTGGG CTCCTGGCTA CTCGCCCGCA TCCTGGCTTG GACCTATGCC 
TTCTATAACA ACTGCCGCCG GCTCCAGTGT TTCCCACAGC CCCCAAAACG GAACTGGTTT 
TGGGGTCACC TGGGCCTGAT CACTCCTACA GAGGAGGGCT TGAAGAACTC GACCCAGATG 
TCGGCCACCT ATTCCCAGGG CTTTACGGTA TGGCTGGGTC CCATCATCCC CTTCATCGTT 
TTATGCCACC CTGACACCAT CCGGTCTATC ACCAATGCCT CAGCTGCCAT TGCACCCAAG 
GATAATCTCT TCATCAGGTT CCTGAAGCCC TGGCTGGGAG AAGGGATACT GCTGAGTGGC 
GGTGACAAGT GGAGCCGCCA CCGTCGGATG CTGACGCCCG CCTTCCATTT CAACATCCTG 
AAGTCCTATA TAACGATCTT CAACAAGAGT GCAAACATCA TGCTTGACAA GTGGCAGCAC 
CTGGCCTCAG AGGGCAGCAG TCGTCTGGAC ATGTTTGAGC ACATCAGCCT CATGACCTTG 
GACAGTCTAC AGAAATGCAT CTTCAGCTTT GACAGCCATT GTCAGGAGAG GCCCAGTGAA 
TATATTGCCA CCATCTTGGA GCTCAGTGCC CTTGTAGAGA AAAGAAGCCA GCATATCCTC 
CAGCACATGG ACTTTCTGTA TTACCTCTCC CATGACGGGC GGCGCTTCCA CAGGGCCTGC 
CGCCTGGTGC ATGACTTCAC AGACGCTGTC ATCCGGGAGC GGCGTCGCAC CCTCCCCACT 
CAGGGTATTG ATGATTTTTT CAAAGACAAA GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTTCTGC TGAGCAAGGA TGAAGATGGG AAGGCATTGT CAGATGAGGA TATAAGAGCA 
GAGGCTGACA CCTTCATGTT TGGAGGCCAT GACACCACGG CCAGTGGCCT CTCCTGGGTC 
CTGTACAACC TTGCGAGGCA CCCAGAATAC CAGGAGCGCT GCCGACAGGA GGTGCAAGAG 
CTTCTGAAGG ACCGCGATCC TAAAGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCGTGAAGGA GAGCCTGAGG TTACATCCCC CAGCTCCCTT CATCTCCCGA 
TGCTGCACCC AGGACATTGT TCTCCCAGAT GGCCGAGTCA TCCCCAAAGG CATTACCTGC 
CTCATCGATA TTATAGGGGT CCATCACAAC CCAACTGTGT GGCCGGATCC TGAGGTCTAC 
GACCCCTTCC GCTTTGACCC AGAGAACAGC AAGGGGAGGT CACCTCTGGC TTTTATTCCT 
TTCTCCGCAG GGCCCAGGAA CTGCATCGGG CAGGCGTTCG CCATGGCGGA GATGAAAGTG 
GTCCTGGCGT TGATGCTGCT GCACTTCCGG TTCCTGCCAG ACCACACTGA GCCCCGCAGG 
AAGCTGGAAT TGATCATGCG CGCCGAGGGC GGGCTTTGGC TGCGGGTGGA GCCCCTGAAT 
GTAAGCTTGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_076433.3
CDS120..1694
Translation

Target ORF information:

RefSeq Version NM_023944.3
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_023944.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCGCTGC TGAGCCTGCC CTGGCTGGGC CTCAGACCGG TGGCAACGTC CCCATGGCTA 
CTCCTGCTGC TGGTTGTGGG CTCCTGGCTA CTCGCCCGCA TCCTGGCTTG GACCTATGCC 
TTCTATAACA ACTGCCGCCG GCTCCAGTGT TTCCCACAGC CCCCAAAACG GAACTGGTTT 
TGGGGTCACC TGGGCCTGAT CACTCCTACA GAGGAGGGCT TGAAGAACTC GACCCAGATG 
TCGGCCACCT ATTCCCAGGG CTTTACGGTA TGGCTGGGTC CCATCATCCC CTTCATCGTT 
TTATGCCACC CTGACACCAT CCGGTCTATC ACCAATGCCT CAGCTGCCAT TGCACCCAAG 
GATAATCTCT TCATCAGGTT CCTGAAGCCC TGGCTGGGAG AAGGGATACT GCTGAGTGGC 
GGTGACAAGT GGAGCCGCCA CCGTCGGATG CTGACGCCCG CCTTCCATTT CAACATCCTG 
AAGTCCTATA TAACGATCTT CAACAAGAGT GCAAACATCA TGCTTGACAA GTGGCAGCAC 
CTGGCCTCAG AGGGCAGCAG TCGTCTGGAC ATGTTTGAGC ACATCAGCCT CATGACCTTG 
GACAGTCTAC AGAAATGCAT CTTCAGCTTT GACAGCCATT GTCAGGAGAG GCCCAGTGAA 
TATATTGCCA CCATCTTGGA GCTCAGTGCC CTTGTAGAGA AAAGAAGCCA GCATATCCTC 
CAGCACATGG ACTTTCTGTA TTACCTCTCC CATGACGGGC GGCGCTTCCA CAGGGCCTGC 
CGCCTGGTGC ATGACTTCAC AGACGCTGTC ATCCGGGAGC GGCGTCGCAC CCTCCCCACT 
CAGGGTATTG ATGATTTTTT CAAAGACAAA GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTTCTGC TGAGCAAGGA TGAAGATGGG AAGGCATTGT CAGATGAGGA TATAAGAGCA 
GAGGCTGACA CCTTCATGTT TGGAGGCCAT GACACCACGG CCAGTGGCCT CTCCTGGGTC 
CTGTACAACC TTGCGAGGCA CCCAGAATAC CAGGAGCGCT GCCGACAGGA GGTGCAAGAG 
CTTCTGAAGG ACCGCGATCC TAAAGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCGTGAAGGA GAGCCTGAGG TTACATCCCC CAGCTCCCTT CATCTCCCGA 
TGCTGCACCC AGGACATTGT TCTCCCAGAT GGCCGAGTCA TCCCCAAAGG CATTACCTGC 
CTCATCGATA TTATAGGGGT CCATCACAAC CCAACTGTGT GGCCGGATCC TGAGGTCTAC 
GACCCCTTCC GCTTTGACCC AGAGAACAGC AAGGGGAGGT CACCTCTGGC TTTTATTCCT 
TTCTCCGCAG GGCCCAGGAA CTGCATCGGG CAGGCGTTCG CCATGGCGGA GATGAAAGTG 
GTCCTGGCGT TGATGCTGCT GCACTTCCGG TTCCTGCCAG ACCACACTGA GCCCCGCAGG 
AAGCTGGAAT TGATCATGCG CGCCGAGGGC GGGCTTTGGC TGCGGGTGGA GCCCCTGAAT 
GTAAGCTTGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21164
Clone ID Related Accession (Same CDS sequence) NM_023944.3 , NM_023944.4
Accession Version NM_023944.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-31
Organism Homo sapiens(human)
Product cytochrome P450 4F12
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122702.2, DC418592.1, AB035130.1 and DR422336.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_023944.3. Transcript Variant: This variant (1) represents the shorter transcript but encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035130.1, AB035131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000550308.6/ ENSP00000448998.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCGCTGC TGAGCCTGCC CTGGCTGGGC CTCAGACCGG TGGCAACGTC CCCATGGCTA 
CTCCTGCTGC TGGTTGTGGG CTCCTGGCTA CTCGCCCGCA TCCTGGCTTG GACCTATGCC 
TTCTATAACA ACTGCCGCCG GCTCCAGTGT TTCCCACAGC CCCCAAAACG GAACTGGTTT 
TGGGGTCACC TGGGCCTGAT CACTCCTACA GAGGAGGGCT TGAAGAACTC GACCCAGATG 
TCGGCCACCT ATTCCCAGGG CTTTACGGTA TGGCTGGGTC CCATCATCCC CTTCATCGTT 
TTATGCCACC CTGACACCAT CCGGTCTATC ACCAATGCCT CAGCTGCCAT TGCACCCAAG 
GATAATCTCT TCATCAGGTT CCTGAAGCCC TGGCTGGGAG AAGGGATACT GCTGAGTGGC 
GGTGACAAGT GGAGCCGCCA CCGTCGGATG CTGACGCCCG CCTTCCATTT CAACATCCTG 
AAGTCCTATA TAACGATCTT CAACAAGAGT GCAAACATCA TGCTTGACAA GTGGCAGCAC 
CTGGCCTCAG AGGGCAGCAG TCGTCTGGAC ATGTTTGAGC ACATCAGCCT CATGACCTTG 
GACAGTCTAC AGAAATGCAT CTTCAGCTTT GACAGCCATT GTCAGGAGAG GCCCAGTGAA 
TATATTGCCA CCATCTTGGA GCTCAGTGCC CTTGTAGAGA AAAGAAGCCA GCATATCCTC 
CAGCACATGG ACTTTCTGTA TTACCTCTCC CATGACGGGC GGCGCTTCCA CAGGGCCTGC 
CGCCTGGTGC ATGACTTCAC AGACGCTGTC ATCCGGGAGC GGCGTCGCAC CCTCCCCACT 
CAGGGTATTG ATGATTTTTT CAAAGACAAA GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTTCTGC TGAGCAAGGA TGAAGATGGG AAGGCATTGT CAGATGAGGA TATAAGAGCA 
GAGGCTGACA CCTTCATGTT TGGAGGCCAT GACACCACGG CCAGTGGCCT CTCCTGGGTC 
CTGTACAACC TTGCGAGGCA CCCAGAATAC CAGGAGCGCT GCCGACAGGA GGTGCAAGAG 
CTTCTGAAGG ACCGCGATCC TAAAGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCGTGAAGGA GAGCCTGAGG TTACATCCCC CAGCTCCCTT CATCTCCCGA 
TGCTGCACCC AGGACATTGT TCTCCCAGAT GGCCGAGTCA TCCCCAAAGG CATTACCTGC 
CTCATCGATA TTATAGGGGT CCATCACAAC CCAACTGTGT GGCCGGATCC TGAGGTCTAC 
GACCCCTTCC GCTTTGACCC AGAGAACAGC AAGGGGAGGT CACCTCTGGC TTTTATTCCT 
TTCTCCGCAG GGCCCAGGAA CTGCATCGGG CAGGCGTTCG CCATGGCGGA GATGAAAGTG 
GTCCTGGCGT TGATGCTGCT GCACTTCCGG TTCCTGCCAG ACCACACTGA GCCCCGCAGG 
AAGCTGGAAT TGATCATGCG CGCCGAGGGC GGGCTTTGGC TGCGGGTGGA GCCCCTGAAT 
GTAAGCTTGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_076433.3
CDS51..1625
Translation

Target ORF information:

RefSeq Version NM_023944.4
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_023944.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCGCTGC TGAGCCTGCC CTGGCTGGGC CTCAGACCGG TGGCAACGTC CCCATGGCTA 
CTCCTGCTGC TGGTTGTGGG CTCCTGGCTA CTCGCCCGCA TCCTGGCTTG GACCTATGCC 
TTCTATAACA ACTGCCGCCG GCTCCAGTGT TTCCCACAGC CCCCAAAACG GAACTGGTTT 
TGGGGTCACC TGGGCCTGAT CACTCCTACA GAGGAGGGCT TGAAGAACTC GACCCAGATG 
TCGGCCACCT ATTCCCAGGG CTTTACGGTA TGGCTGGGTC CCATCATCCC CTTCATCGTT 
TTATGCCACC CTGACACCAT CCGGTCTATC ACCAATGCCT CAGCTGCCAT TGCACCCAAG 
GATAATCTCT TCATCAGGTT CCTGAAGCCC TGGCTGGGAG AAGGGATACT GCTGAGTGGC 
GGTGACAAGT GGAGCCGCCA CCGTCGGATG CTGACGCCCG CCTTCCATTT CAACATCCTG 
AAGTCCTATA TAACGATCTT CAACAAGAGT GCAAACATCA TGCTTGACAA GTGGCAGCAC 
CTGGCCTCAG AGGGCAGCAG TCGTCTGGAC ATGTTTGAGC ACATCAGCCT CATGACCTTG 
GACAGTCTAC AGAAATGCAT CTTCAGCTTT GACAGCCATT GTCAGGAGAG GCCCAGTGAA 
TATATTGCCA CCATCTTGGA GCTCAGTGCC CTTGTAGAGA AAAGAAGCCA GCATATCCTC 
CAGCACATGG ACTTTCTGTA TTACCTCTCC CATGACGGGC GGCGCTTCCA CAGGGCCTGC 
CGCCTGGTGC ATGACTTCAC AGACGCTGTC ATCCGGGAGC GGCGTCGCAC CCTCCCCACT 
CAGGGTATTG ATGATTTTTT CAAAGACAAA GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTTCTGC TGAGCAAGGA TGAAGATGGG AAGGCATTGT CAGATGAGGA TATAAGAGCA 
GAGGCTGACA CCTTCATGTT TGGAGGCCAT GACACCACGG CCAGTGGCCT CTCCTGGGTC 
CTGTACAACC TTGCGAGGCA CCCAGAATAC CAGGAGCGCT GCCGACAGGA GGTGCAAGAG 
CTTCTGAAGG ACCGCGATCC TAAAGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCGTGAAGGA GAGCCTGAGG TTACATCCCC CAGCTCCCTT CATCTCCCGA 
TGCTGCACCC AGGACATTGT TCTCCCAGAT GGCCGAGTCA TCCCCAAAGG CATTACCTGC 
CTCATCGATA TTATAGGGGT CCATCACAAC CCAACTGTGT GGCCGGATCC TGAGGTCTAC 
GACCCCTTCC GCTTTGACCC AGAGAACAGC AAGGGGAGGT CACCTCTGGC TTTTATTCCT 
TTCTCCGCAG GGCCCAGGAA CTGCATCGGG CAGGCGTTCG CCATGGCGGA GATGAAAGTG 
GTCCTGGCGT TGATGCTGCT GCACTTCCGG TTCCTGCCAG ACCACACTGA GCCCCGCAGG 
AAGCTGGAAT TGATCATGCG CGCCGAGGGC GGGCTTTGGC TGCGGGTGGA GCCCCTGAAT 
GTAAGCTTGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51770
Clone ID Related Accession (Same CDS sequence) XM_006722850.3 , XM_011528202.2 , XM_011528208.2 , XM_011528202.3
Accession Version XM_006722850.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722913.1
CDS452..1579
Translation

Target ORF information:

RefSeq Version XM_006722850.3
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006722850.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51770
Clone ID Related Accession (Same CDS sequence) XM_006722850.3 , XM_011528202.2 , XM_011528208.2 , XM_011528202.3
Accession Version XM_011528202.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526504.1
CDS87..1214
Translation

Target ORF information:

RefSeq Version XM_011528202.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528202.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51770
Clone ID Related Accession (Same CDS sequence) XM_006722850.3 , XM_011528202.2 , XM_011528208.2 , XM_011528202.3
Accession Version XM_011528208.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526510.1
CDS206..1333
Translation

Target ORF information:

RefSeq Version XM_011528208.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528208.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51770
Clone ID Related Accession (Same CDS sequence) XM_006722850.3 , XM_011528202.2 , XM_011528208.2 , XM_011528202.3
Accession Version XM_011528202.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528202.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526504.1
CDS86..1213
Translation

Target ORF information:

RefSeq Version XM_011528202.3
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528202.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGACGC CCGCCTTCCA TTTCAACATC CTGAAGTCCT ATATAACGAT CTTCAACAAG 
AGTGCAAACA TCATGCTTGA CAAGTGGCAG CACCTGGCCT CAGAGGGCAG CAGTCGTCTG 
GACATGTTTG AGCACATCAG CCTCATGACC TTGGACAGTC TACAGAAATG CATCTTCAGC 
TTTGACAGCC ATTGTCAGGA GAGGCCCAGT GAATATATTG CCACCATCTT GGAGCTCAGT 
GCCCTTGTAG AGAAAAGAAG CCAGCATATC CTCCAGCACA TGGACTTTCT GTATTACCTC 
TCCCATGACG GGCGGCGCTT CCACAGGGCC TGCCGCCTGG TGCATGACTT CACAGACGCT 
GTCATCCGGG AGCGGCGTCG CACCCTCCCC ACTCAGGGTA TTGATGATTT TTTCAAAGAC 
AAAGCCAAGT CCAAGACTTT GGATTTCATT GATGTGCTTC TGCTGAGCAA GGATGAAGAT 
GGGAAGGCAT TGTCAGATGA GGATATAAGA GCAGAGGCTG ACACCTTCAT GTTTGGAGGC 
CATGACACCA CGGCCAGTGG CCTCTCCTGG GTCCTGTACA ACCTTGCGAG GCACCCAGAA 
TACCAGGAGC GCTGCCGACA GGAGGTGCAA GAGCTTCTGA AGGACCGCGA TCCTAAAGAG 
ATTGAATGGG ACGACCTGGC CCAGCTGCCC TTCCTGACCA TGTGCGTGAA GGAGAGCCTG 
AGGTTACATC CCCCAGCTCC CTTCATCTCC CGATGCTGCA CCCAGGACAT TGTTCTCCCA 
GATGGCCGAG TCATCCCCAA AGGCATTACC TGCCTCATCG ATATTATAGG GGTCCATCAC 
AACCCAACTG TGTGGCCGGA TCCTGAGGTC TACGACCCCT TCCGCTTTGA CCCAGAGAAC 
AGCAAGGGGA GGTCACCTCT GGCTTTTATT CCTTTCTCCG CAGGGCCCAG GAACTGCATC 
GGGCAGGCGT TCGCCATGGC GGAGATGAAA GTGGTCCTGG CGTTGATGCT GCTGCACTTC 
CGGTTCCTGC CAGACCACAC TGAGCCCCGC AGGAAGCTGG AATTGATCAT GCGCGCCGAG 
GGCGGGCTTT GGCTGCGGGT GGAGCCCCTG AATGTAAGCT TGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51771
Clone ID Related Accession (Same CDS sequence) XM_011528207.2
Accession Version XM_011528207.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1005bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTTTGAGC ACATCAGCCT CATGACCTTG GACAGTCTAC AGAAATGCAT CTTCAGCTTT 
GACAGCCATT GTCAGGAGAG GCCCAGTGAA TATATTGCCA CCATCTTGGA GCTCAGTGCC 
CTTGTAGAGA AAAGAAGCCA GCATATCCTC CAGCACATGG ACTTTCTGTA TTACCTCTCC 
CATGACGGGC GGCGCTTCCA CAGGGCCTGC CGCCTGGTGC ATGACTTCAC AGACGCTGTC 
ATCCGGGAGC GGCGTCGCAC CCTCCCCACT CAGGGTATTG ATGATTTTTT CAAAGACAAA 
GCCAAGTCCA AGACTTTGGA TTTCATTGAT GTGCTTCTGC TGAGCAAGGA TGAAGATGGG 
AAGGCATTGT CAGATGAGGA TATAAGAGCA GAGGCTGACA CCTTCATGTT TGGAGGCCAT 
GACACCACGG CCAGTGGCCT CTCCTGGGTC CTGTACAACC TTGCGAGGCA CCCAGAATAC 
CAGGAGCGCT GCCGACAGGA GGTGCAAGAG CTTCTGAAGG ACCGCGATCC TAAAGAGATT 
GAATGGGACG ACCTGGCCCA GCTGCCCTTC CTGACCATGT GCGTGAAGGA GAGCCTGAGG 
TTACATCCCC CAGCTCCCTT CATCTCCCGA TGCTGCACCC AGGACATTGT TCTCCCAGAT 
GGCCGAGTCA TCCCCAAAGG CATTACCTGC CTCATCGATA TTATAGGGGT CCATCACAAC 
CCAACTGTGT GGCCGGATCC TGAGGTCTAC GACCCCTTCC GCTTTGACCC AGAGAACAGC 
AAGGGGAGGT CACCTCTGGC TTTTATTCCT TTCTCCGCAG GGCCCAGGAA CTGCATCGGG 
CAGGCGTTCG CCATGGCGGA GATGAAAGTG GTCCTGGCGT TGATGCTGCT GCACTTCCGG 
TTCCTGCCAG ACCACACTGA GCCCCGCAGG AAGCTGGAAT TGATCATGCG CGCCGAGGGC 
GGGCTTTGGC TGCGGGTGGA GCCCCTGAAT GTAAGCTTGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526509.1
CDS1819..2823
Translation

Target ORF information:

RefSeq Version XM_011528207.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528207.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTTTGAGC ACATCAGCCT CATGACCTTG GACAGTCTAC AGAAATGCAT CTTCAGCTTT 
GACAGCCATT GTCAGGAGAG GCCCAGTGAA TATATTGCCA CCATCTTGGA GCTCAGTGCC 
CTTGTAGAGA AAAGAAGCCA GCATATCCTC CAGCACATGG ACTTTCTGTA TTACCTCTCC 
CATGACGGGC GGCGCTTCCA CAGGGCCTGC CGCCTGGTGC ATGACTTCAC AGACGCTGTC 
ATCCGGGAGC GGCGTCGCAC CCTCCCCACT CAGGGTATTG ATGATTTTTT CAAAGACAAA 
GCCAAGTCCA AGACTTTGGA TTTCATTGAT GTGCTTCTGC TGAGCAAGGA TGAAGATGGG 
AAGGCATTGT CAGATGAGGA TATAAGAGCA GAGGCTGACA CCTTCATGTT TGGAGGCCAT 
GACACCACGG CCAGTGGCCT CTCCTGGGTC CTGTACAACC TTGCGAGGCA CCCAGAATAC 
CAGGAGCGCT GCCGACAGGA GGTGCAAGAG CTTCTGAAGG ACCGCGATCC TAAAGAGATT 
GAATGGGACG ACCTGGCCCA GCTGCCCTTC CTGACCATGT GCGTGAAGGA GAGCCTGAGG 
TTACATCCCC CAGCTCCCTT CATCTCCCGA TGCTGCACCC AGGACATTGT TCTCCCAGAT 
GGCCGAGTCA TCCCCAAAGG CATTACCTGC CTCATCGATA TTATAGGGGT CCATCACAAC 
CCAACTGTGT GGCCGGATCC TGAGGTCTAC GACCCCTTCC GCTTTGACCC AGAGAACAGC 
AAGGGGAGGT CACCTCTGGC TTTTATTCCT TTCTCCGCAG GGCCCAGGAA CTGCATCGGG 
CAGGCGTTCG CCATGGCGGA GATGAAAGTG GTCCTGGCGT TGATGCTGCT GCACTTCCGG 
TTCCTGCCAG ACCACACTGA GCCCCGCAGG AAGCTGGAAT TGATCATGCG CGCCGAGGGC 
GGGCTTTGGC TGCGGGTGGA GCCCCTGAAT GTAAGCTTGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71682
Clone ID Related Accession (Same CDS sequence) XM_011528204.2 , XM_011528203.2 , XM_011528205.2
Accession Version XM_011528204.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCAGG ACAAGTGGCA GCACCTGGCC TCAGAGGGCA GCAGTCGTCT GGACATGTTT 
GAGCACATCA GCCTCATGAC CTTGGACAGT CTACAGAAAT GCATCTTCAG CTTTGACAGC 
CATTGTCAGG AGAGGCCCAG TGAATATATT GCCACCATCT TGGAGCTCAG TGCCCTTGTA 
GAGAAAAGAA GCCAGCATAT CCTCCAGCAC ATGGACTTTC TGTATTACCT CTCCCATGAC 
GGGCGGCGCT TCCACAGGGC CTGCCGCCTG GTGCATGACT TCACAGACGC TGTCATCCGG 
GAGCGGCGTC GCACCCTCCC CACTCAGGGT ATTGATGATT TTTTCAAAGA CAAAGCCAAG 
TCCAAGACTT TGGATTTCAT TGATGTGCTT CTGCTGAGCA AGGATGAAGA TGGGAAGGCA 
TTGTCAGATG AGGATATAAG AGCAGAGGCT GACACCTTCA TGTTTGGAGG CCATGACACC 
ACGGCCAGTG GCCTCTCCTG GGTCCTGTAC AACCTTGCGA GGCACCCAGA ATACCAGGAG 
CGCTGCCGAC AGGAGGTGCA AGAGCTTCTG AAGGACCGCG ATCCTAAAGA GATTGAATGG 
GACGACCTGG CCCAGCTGCC CTTCCTGACC ATGTGCGTGA AGGAGAGCCT GAGGTTACAT 
CCCCCAGCTC CCTTCATCTC CCGATGCTGC ACCCAGGACA TTGTTCTCCC AGATGGCCGA 
GTCATCCCCA AAGGCATTAC CTGCCTCATC GATATTATAG GGGTCCATCA CAACCCAACT 
GTGTGGCCGG ATCCTGAGGT CTACGACCCC TTCCGCTTTG ACCCAGAGAA CAGCAAGGGG 
AGGTCACCTC TGGCTTTTAT TCCTTTCTCC GCAGGGCCCA GGAACTGCAT CGGGCAGGCG 
TTCGCCATGG CGGAGATGAA AGTGGTCCTG GCGTTGATGC TGCTGCACTT CCGGTTCCTG 
CCAGACCACA CTGAGCCCCG CAGGAAGCTG GAATTGATCA TGCGCGCCGA GGGCGGGCTT 
TGGCTGCGGG TGGAGCCCCT GAATGTAAGC TTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526506.1
CDS857..1915
Translation

Target ORF information:

RefSeq Version XM_011528204.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528204.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCAGG ACAAGTGGCA GCACCTGGCC TCAGAGGGCA GCAGTCGTCT GGACATGTTT 
GAGCACATCA GCCTCATGAC CTTGGACAGT CTACAGAAAT GCATCTTCAG CTTTGACAGC 
CATTGTCAGG AGAGGCCCAG TGAATATATT GCCACCATCT TGGAGCTCAG TGCCCTTGTA 
GAGAAAAGAA GCCAGCATAT CCTCCAGCAC ATGGACTTTC TGTATTACCT CTCCCATGAC 
GGGCGGCGCT TCCACAGGGC CTGCCGCCTG GTGCATGACT TCACAGACGC TGTCATCCGG 
GAGCGGCGTC GCACCCTCCC CACTCAGGGT ATTGATGATT TTTTCAAAGA CAAAGCCAAG 
TCCAAGACTT TGGATTTCAT TGATGTGCTT CTGCTGAGCA AGGATGAAGA TGGGAAGGCA 
TTGTCAGATG AGGATATAAG AGCAGAGGCT GACACCTTCA TGTTTGGAGG CCATGACACC 
ACGGCCAGTG GCCTCTCCTG GGTCCTGTAC AACCTTGCGA GGCACCCAGA ATACCAGGAG 
CGCTGCCGAC AGGAGGTGCA AGAGCTTCTG AAGGACCGCG ATCCTAAAGA GATTGAATGG 
GACGACCTGG CCCAGCTGCC CTTCCTGACC ATGTGCGTGA AGGAGAGCCT GAGGTTACAT 
CCCCCAGCTC CCTTCATCTC CCGATGCTGC ACCCAGGACA TTGTTCTCCC AGATGGCCGA 
GTCATCCCCA AAGGCATTAC CTGCCTCATC GATATTATAG GGGTCCATCA CAACCCAACT 
GTGTGGCCGG ATCCTGAGGT CTACGACCCC TTCCGCTTTG ACCCAGAGAA CAGCAAGGGG 
AGGTCACCTC TGGCTTTTAT TCCTTTCTCC GCAGGGCCCA GGAACTGCAT CGGGCAGGCG 
TTCGCCATGG CGGAGATGAA AGTGGTCCTG GCGTTGATGC TGCTGCACTT CCGGTTCCTG 
CCAGACCACA CTGAGCCCCG CAGGAAGCTG GAATTGATCA TGCGCGCCGA GGGCGGGCTT 
TGGCTGCGGG TGGAGCCCCT GAATGTAAGC TTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71682
Clone ID Related Accession (Same CDS sequence) XM_011528204.2 , XM_011528203.2 , XM_011528205.2
Accession Version XM_011528203.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCAGG ACAAGTGGCA GCACCTGGCC TCAGAGGGCA GCAGTCGTCT GGACATGTTT 
GAGCACATCA GCCTCATGAC CTTGGACAGT CTACAGAAAT GCATCTTCAG CTTTGACAGC 
CATTGTCAGG AGAGGCCCAG TGAATATATT GCCACCATCT TGGAGCTCAG TGCCCTTGTA 
GAGAAAAGAA GCCAGCATAT CCTCCAGCAC ATGGACTTTC TGTATTACCT CTCCCATGAC 
GGGCGGCGCT TCCACAGGGC CTGCCGCCTG GTGCATGACT TCACAGACGC TGTCATCCGG 
GAGCGGCGTC GCACCCTCCC CACTCAGGGT ATTGATGATT TTTTCAAAGA CAAAGCCAAG 
TCCAAGACTT TGGATTTCAT TGATGTGCTT CTGCTGAGCA AGGATGAAGA TGGGAAGGCA 
TTGTCAGATG AGGATATAAG AGCAGAGGCT GACACCTTCA TGTTTGGAGG CCATGACACC 
ACGGCCAGTG GCCTCTCCTG GGTCCTGTAC AACCTTGCGA GGCACCCAGA ATACCAGGAG 
CGCTGCCGAC AGGAGGTGCA AGAGCTTCTG AAGGACCGCG ATCCTAAAGA GATTGAATGG 
GACGACCTGG CCCAGCTGCC CTTCCTGACC ATGTGCGTGA AGGAGAGCCT GAGGTTACAT 
CCCCCAGCTC CCTTCATCTC CCGATGCTGC ACCCAGGACA TTGTTCTCCC AGATGGCCGA 
GTCATCCCCA AAGGCATTAC CTGCCTCATC GATATTATAG GGGTCCATCA CAACCCAACT 
GTGTGGCCGG ATCCTGAGGT CTACGACCCC TTCCGCTTTG ACCCAGAGAA CAGCAAGGGG 
AGGTCACCTC TGGCTTTTAT TCCTTTCTCC GCAGGGCCCA GGAACTGCAT CGGGCAGGCG 
TTCGCCATGG CGGAGATGAA AGTGGTCCTG GCGTTGATGC TGCTGCACTT CCGGTTCCTG 
CCAGACCACA CTGAGCCCCG CAGGAAGCTG GAATTGATCA TGCGCGCCGA GGGCGGGCTT 
TGGCTGCGGG TGGAGCCCCT GAATGTAAGC TTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526505.1
CDS1378..2436
Translation

Target ORF information:

RefSeq Version XM_011528203.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528203.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCAGG ACAAGTGGCA GCACCTGGCC TCAGAGGGCA GCAGTCGTCT GGACATGTTT 
GAGCACATCA GCCTCATGAC CTTGGACAGT CTACAGAAAT GCATCTTCAG CTTTGACAGC 
CATTGTCAGG AGAGGCCCAG TGAATATATT GCCACCATCT TGGAGCTCAG TGCCCTTGTA 
GAGAAAAGAA GCCAGCATAT CCTCCAGCAC ATGGACTTTC TGTATTACCT CTCCCATGAC 
GGGCGGCGCT TCCACAGGGC CTGCCGCCTG GTGCATGACT TCACAGACGC TGTCATCCGG 
GAGCGGCGTC GCACCCTCCC CACTCAGGGT ATTGATGATT TTTTCAAAGA CAAAGCCAAG 
TCCAAGACTT TGGATTTCAT TGATGTGCTT CTGCTGAGCA AGGATGAAGA TGGGAAGGCA 
TTGTCAGATG AGGATATAAG AGCAGAGGCT GACACCTTCA TGTTTGGAGG CCATGACACC 
ACGGCCAGTG GCCTCTCCTG GGTCCTGTAC AACCTTGCGA GGCACCCAGA ATACCAGGAG 
CGCTGCCGAC AGGAGGTGCA AGAGCTTCTG AAGGACCGCG ATCCTAAAGA GATTGAATGG 
GACGACCTGG CCCAGCTGCC CTTCCTGACC ATGTGCGTGA AGGAGAGCCT GAGGTTACAT 
CCCCCAGCTC CCTTCATCTC CCGATGCTGC ACCCAGGACA TTGTTCTCCC AGATGGCCGA 
GTCATCCCCA AAGGCATTAC CTGCCTCATC GATATTATAG GGGTCCATCA CAACCCAACT 
GTGTGGCCGG ATCCTGAGGT CTACGACCCC TTCCGCTTTG ACCCAGAGAA CAGCAAGGGG 
AGGTCACCTC TGGCTTTTAT TCCTTTCTCC GCAGGGCCCA GGAACTGCAT CGGGCAGGCG 
TTCGCCATGG CGGAGATGAA AGTGGTCCTG GCGTTGATGC TGCTGCACTT CCGGTTCCTG 
CCAGACCACA CTGAGCCCCG CAGGAAGCTG GAATTGATCA TGCGCGCCGA GGGCGGGCTT 
TGGCTGCGGG TGGAGCCCCT GAATGTAAGC TTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71682
Clone ID Related Accession (Same CDS sequence) XM_011528204.2 , XM_011528203.2 , XM_011528205.2
Accession Version XM_011528205.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCAGG ACAAGTGGCA GCACCTGGCC TCAGAGGGCA GCAGTCGTCT GGACATGTTT 
GAGCACATCA GCCTCATGAC CTTGGACAGT CTACAGAAAT GCATCTTCAG CTTTGACAGC 
CATTGTCAGG AGAGGCCCAG TGAATATATT GCCACCATCT TGGAGCTCAG TGCCCTTGTA 
GAGAAAAGAA GCCAGCATAT CCTCCAGCAC ATGGACTTTC TGTATTACCT CTCCCATGAC 
GGGCGGCGCT TCCACAGGGC CTGCCGCCTG GTGCATGACT TCACAGACGC TGTCATCCGG 
GAGCGGCGTC GCACCCTCCC CACTCAGGGT ATTGATGATT TTTTCAAAGA CAAAGCCAAG 
TCCAAGACTT TGGATTTCAT TGATGTGCTT CTGCTGAGCA AGGATGAAGA TGGGAAGGCA 
TTGTCAGATG AGGATATAAG AGCAGAGGCT GACACCTTCA TGTTTGGAGG CCATGACACC 
ACGGCCAGTG GCCTCTCCTG GGTCCTGTAC AACCTTGCGA GGCACCCAGA ATACCAGGAG 
CGCTGCCGAC AGGAGGTGCA AGAGCTTCTG AAGGACCGCG ATCCTAAAGA GATTGAATGG 
GACGACCTGG CCCAGCTGCC CTTCCTGACC ATGTGCGTGA AGGAGAGCCT GAGGTTACAT 
CCCCCAGCTC CCTTCATCTC CCGATGCTGC ACCCAGGACA TTGTTCTCCC AGATGGCCGA 
GTCATCCCCA AAGGCATTAC CTGCCTCATC GATATTATAG GGGTCCATCA CAACCCAACT 
GTGTGGCCGG ATCCTGAGGT CTACGACCCC TTCCGCTTTG ACCCAGAGAA CAGCAAGGGG 
AGGTCACCTC TGGCTTTTAT TCCTTTCTCC GCAGGGCCCA GGAACTGCAT CGGGCAGGCG 
TTCGCCATGG CGGAGATGAA AGTGGTCCTG GCGTTGATGC TGCTGCACTT CCGGTTCCTG 
CCAGACCACA CTGAGCCCCG CAGGAAGCTG GAATTGATCA TGCGCGCCGA GGGCGGGCTT 
TGGCTGCGGG TGGAGCCCCT GAATGTAAGC TTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526507.1
CDS69..1127
Translation

Target ORF information:

RefSeq Version XM_011528205.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011528205.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCAGG ACAAGTGGCA GCACCTGGCC TCAGAGGGCA GCAGTCGTCT GGACATGTTT 
GAGCACATCA GCCTCATGAC CTTGGACAGT CTACAGAAAT GCATCTTCAG CTTTGACAGC 
CATTGTCAGG AGAGGCCCAG TGAATATATT GCCACCATCT TGGAGCTCAG TGCCCTTGTA 
GAGAAAAGAA GCCAGCATAT CCTCCAGCAC ATGGACTTTC TGTATTACCT CTCCCATGAC 
GGGCGGCGCT TCCACAGGGC CTGCCGCCTG GTGCATGACT TCACAGACGC TGTCATCCGG 
GAGCGGCGTC GCACCCTCCC CACTCAGGGT ATTGATGATT TTTTCAAAGA CAAAGCCAAG 
TCCAAGACTT TGGATTTCAT TGATGTGCTT CTGCTGAGCA AGGATGAAGA TGGGAAGGCA 
TTGTCAGATG AGGATATAAG AGCAGAGGCT GACACCTTCA TGTTTGGAGG CCATGACACC 
ACGGCCAGTG GCCTCTCCTG GGTCCTGTAC AACCTTGCGA GGCACCCAGA ATACCAGGAG 
CGCTGCCGAC AGGAGGTGCA AGAGCTTCTG AAGGACCGCG ATCCTAAAGA GATTGAATGG 
GACGACCTGG CCCAGCTGCC CTTCCTGACC ATGTGCGTGA AGGAGAGCCT GAGGTTACAT 
CCCCCAGCTC CCTTCATCTC CCGATGCTGC ACCCAGGACA TTGTTCTCCC AGATGGCCGA 
GTCATCCCCA AAGGCATTAC CTGCCTCATC GATATTATAG GGGTCCATCA CAACCCAACT 
GTGTGGCCGG ATCCTGAGGT CTACGACCCC TTCCGCTTTG ACCCAGAGAA CAGCAAGGGG 
AGGTCACCTC TGGCTTTTAT TCCTTTCTCC GCAGGGCCCA GGAACTGCAT CGGGCAGGCG 
TTCGCCATGG CGGAGATGAA AGTGGTCCTG GCGTTGATGC TGCTGCACTT CCGGTTCCTG 
CCAGACCACA CTGAGCCCCG CAGGAAGCTG GAATTGATCA TGCGCGCCGA GGGCGGGCTT 
TGGCTGCGGG TGGAGCCCCT GAATGTAAGC TTGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu88680
Clone ID Related Accession (Same CDS sequence) XM_017027172.1
Accession Version XM_017027172.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F12 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCGCTGC TGAGCCTGCC CTGGCTGGGC CTCAGACCGG TGGCAACGTC CCCATGGCTA 
CTCCTGCTGC TGGTTGTGGG CTCCTGGCTA CTCGCCCGCA TCCTGGCTTG GACCTATGCC 
TTCTATAACA ACTGCCGCCG GCTCCAGTGT TTCCCACAGC CCCCAAAACG GAACTGGTTT 
TGGGGTCACC TGGGCCTGAT CACTCCTACA GAGGAGGGCT TGAAGAACTC GACCCAGATG 
TCGGCCACCT ATTCCCAGGG CTTTACGGTA TGGCTGGGTC CCATCATCCC CTTCATCGTT 
TTATGCCACC CTGACACCAT CCGGTCTATC ACCAATGCCT CAGCTGCCAT TGCACCCAAG 
GATAATCTCT TCATCAGGTT CCTGAAGCCC TGGCTGGGAG AAGGGATACT GCTGAGTGGC 
GGTGACAAGT GGAGCCGCCA CCGTCGGATG CTGACGCCCG CCTTCCATTT CAACATCCTG 
AAGTCCTATA TAACGATCTT CAACAAGAGT GCAAACATCA TGCTTGACAA GTGGCAGCAC 
CTGGCCTCAG AGGGCAGCAG TCGTCTGGAC ATGTTTGAGC ACATCAGCCT CATGACCTTG 
GACAGTCTAC AGAAATGCAT CTTCAGCTTT GACAGCCATT GTCAGGAGAG GCCCAGTGAA 
TATATTGCCA CCATCTTGGA GCTCAGTGCC CTTGTAGAGA AAAGAAGCCA GCATATCCTC 
CAGCACATGG ACTTTCTGTA TTACCTCTCC CATGACGGGC GGCGCTTCCA CAGGGCCTGC 
CGCCTGGTGC ATGACTTCAC AGACGCTGTC ATCCGGGAGC GGCGTCGCAC CCTCCCCACT 
CAGGGTATTG ATGATTTTTT CAAAGACAAA GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTTCTGC TGAGCAAGGA TGAAGATGGG AAGGCATTGT CAGATGAGGA TATAAGAGCA 
GAGGCTGACA CCTTCATGTT TGGAGGCCAT GACACCACGG CCAGTGGCCT CTCCTGGGTC 
CTGTACAACC TTGCGAGGCA CCCAGAATAC CAGGAGCGCT GCCGACAGGA GGTGCAAGAG 
CTTCTGAAGG ACCGCGATCC TAAAGAGATT GAATGGTCTA CGACCCCTTC CGCTTTGACC 
CAGAGAACAG CAAGGGGAGG TCACCTCTGG CTTTTATTCC TTTCTCCGCA GGGCCCAGGA 
ACTGCATCGG GCAGGCGTTC GCCATGGCGG AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016882661.1
CDS62..1297
Translation

Target ORF information:

RefSeq Version XM_017027172.1
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 12 (CYP4F12), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017027172.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCGCTGC TGAGCCTGCC CTGGCTGGGC CTCAGACCGG TGGCAACGTC CCCATGGCTA 
CTCCTGCTGC TGGTTGTGGG CTCCTGGCTA CTCGCCCGCA TCCTGGCTTG GACCTATGCC 
TTCTATAACA ACTGCCGCCG GCTCCAGTGT TTCCCACAGC CCCCAAAACG GAACTGGTTT 
TGGGGTCACC TGGGCCTGAT CACTCCTACA GAGGAGGGCT TGAAGAACTC GACCCAGATG 
TCGGCCACCT ATTCCCAGGG CTTTACGGTA TGGCTGGGTC CCATCATCCC CTTCATCGTT 
TTATGCCACC CTGACACCAT CCGGTCTATC ACCAATGCCT CAGCTGCCAT TGCACCCAAG 
GATAATCTCT TCATCAGGTT CCTGAAGCCC TGGCTGGGAG AAGGGATACT GCTGAGTGGC 
GGTGACAAGT GGAGCCGCCA CCGTCGGATG CTGACGCCCG CCTTCCATTT CAACATCCTG 
AAGTCCTATA TAACGATCTT CAACAAGAGT GCAAACATCA TGCTTGACAA GTGGCAGCAC 
CTGGCCTCAG AGGGCAGCAG TCGTCTGGAC ATGTTTGAGC ACATCAGCCT CATGACCTTG 
GACAGTCTAC AGAAATGCAT CTTCAGCTTT GACAGCCATT GTCAGGAGAG GCCCAGTGAA 
TATATTGCCA CCATCTTGGA GCTCAGTGCC CTTGTAGAGA AAAGAAGCCA GCATATCCTC 
CAGCACATGG ACTTTCTGTA TTACCTCTCC CATGACGGGC GGCGCTTCCA CAGGGCCTGC 
CGCCTGGTGC ATGACTTCAC AGACGCTGTC ATCCGGGAGC GGCGTCGCAC CCTCCCCACT 
CAGGGTATTG ATGATTTTTT CAAAGACAAA GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTTCTGC TGAGCAAGGA TGAAGATGGG AAGGCATTGT CAGATGAGGA TATAAGAGCA 
GAGGCTGACA CCTTCATGTT TGGAGGCCAT GACACCACGG CCAGTGGCCT CTCCTGGGTC 
CTGTACAACC TTGCGAGGCA CCCAGAATAC CAGGAGCGCT GCCGACAGGA GGTGCAAGAG 
CTTCTGAAGG ACCGCGATCC TAAAGAGATT GAATGGTCTA CGACCCCTTC CGCTTTGACC 
CAGAGAACAG CAAGGGGAGG TCACCTCTGG CTTTTATTCC TTTCTCCGCA GGGCCCAGGA 
ACTGCATCGG GCAGGCGTTC GCCATGGCGG AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Terfenadine t-butyl hydroxylation catalyzed by human and marmoset cytochrome P450 3A and 4F enzymes in livers and small intestines.
Xenobiotica; the fate of foreign compounds in biological systems1-6(2017 May)
Uehara S,Yuki Y,Uno Y,Inoue T,Sasaki E,Yamazaki H


book

Inducible CYP4F12 enhances Hepatitis C virus infection via association with viral nonstructural protein 5B.
Biochemical and biophysical research communications471(1)95-102(2016 Feb)
Zhu SL,Wang L,Cao ZY,Wang J,Jing MZ,Xia ZC,Ao F,Ye LB,Liu S,Zhu Y


book

Identification of pregnane-X receptor target genes and coactivator and corepressor binding to promoter elements in human hepatocytes.
Nucleic acids research37(4)1160-73(2009 Mar)
Hariparsad N,Chu X,Yabut J,Labhart P,Hartley DP,Dai X,Evers R


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Human CYP4F12 genetic polymorphism: identification and functional characterization of seven variant allozymes.
Biochemical pharmacology68(12)2417-25(2004 Dec)
Cauffiez C,Klinzig F,Rat E,Tournel G,Allorge D,Chevalier D,Pottier N,Lovecchio T,Colombel JF,Lhermitte M,Lo-Guidice JM,Broly F


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Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants.
Pharmacogenetics14(1)1-18(2004 Jan)
Nelson DR,Zeldin DC,Hoffman SM,Maltais LJ,Wain HM,Nebert DW