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UCHL1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol UCHL1
Entrez Gene ID 7345
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms HEL-117, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, Uch-L1
General protein information
Preferred Names
ubiquitin carboxyl-terminal hydrolase isozyme L1
Names
ubiquitin carboxyl-terminal hydrolase isozyme L1
ubiquitin thioesterase L1
neuron cytoplasmic protein 9.5
ubiquitin C-terminal hydrolase
NP_004172.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4p14

Summary The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

191342

Disorder Html: {Parkinson disease 5, susceptibility to}, 613643 (3); {Parkinson

mRNA and Protein(s)

mRNA Protein Name
NM_004181 NP_004172 ubiquitin carboxyl-terminal hydrolase isozyme L1



Homo sapiens (human) UCHL1 NP_004172.2
Pan troglodytes (chimpanzee) UCHL1 XP_517163.2
Macaca mulatta (Rhesus monkey) LOC701579 XP_001097839.2
Canis lupus familiaris (dog) UCHL1 XP_536245.3
Bos taurus (cattle) UCHL1 NP_001039637.1
Mus musculus (house mouse) Uchl1 NP_035800.2
Rattus norvegicus (Norway rat) Uchl1 NP_058933.2
Gallus gallus (chicken) UCHL1 NP_001073681.1
Danio rerio (zebrafish) uchl1 NP_958885.1
Xenopus (Silurana) tropicalis (western clawed frog) uchl1 NP_001011321.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UCHL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UCHL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19475 NM_004181 Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $216.30
$309.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19475
Clone ID Related Accession (Same CDS sequence) NM_004181
Accession Version NM_004181.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 672bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP202166.1 and BC000332.2. This sequence is a reference standard in the RefSeqGene project. On Jun 12, 2009 this sequence version replaced gi:34147658. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000332.2, BC006305.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGCAGCTCA AGCCGATGGA GATCAACCCC GAGATGCTGA ACAAAGTGCT GTCCCGGCTG 
GGGGTCGCCG GCCAGTGGCG CTTCGTGGAC GTGCTGGGGC TGGAAGAGGA GTCTCTGGGC
TCGGTGCCAG CGCCTGCCTG CGCGCTGCTG CTGCTGTTTC CCCTCACGGC CCAGCATGAG
AACTTCAGGA AAAAGCAGAT TGAAGAGCTG AAGGGACAAG AAGTTAGTCC TAAAGTGTAC
TTCATGAAGC AGACCATTGG GAATTCCTGT GGCACAATCG GACTTATTCA CGCAGTGGCC
AATAATCAAG ACAAACTGGG ATTTGAGGAT GGATCAGTTC TGAAACAGTT TCTTTCTGAA
ACAGAGAAAA TGTCCCCTGA AGACAGAGCA AAATGCTTTG AAAAGAATGA GGCCATACAG
GCAGCCCATG ATGCCGTGGC ACAGGAAGGC CAATGTCGGG TAGATGACAA GGTGAATTTC
CATTTTATTC TGTTTAACAA CGTGGATGGC CACCTCTATG AACTTGATGG ACGAATGCCT
TTTCCGGTGA ACCATGGCGC CAGTTCAGAG GACACCCTGC TGAAGGACGC TGCCAAGGTC
TGCAGAGAAT TCACCGAGCG TGAGCAAGGA GAAGTCCGCT TCTCTGCCGT GGCTCTCTGC
AAGGCAGCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004172.2
CDS97..768
Misc Feature(1)67..69(+)
Misc Feature(2)97..99(+)
Misc Feature(3)109..753(+)
Misc Feature(4)109..711(+)
Misc Feature(5)109..579(+)
Misc Feature(6)109..126(+)
Misc Feature(7)112..114(+)
Misc Feature(8)130..132(+)
Misc Feature(9)346..624(+)
Misc Feature(10)466..468(+)
Misc Feature(11)622..624(+)
Misc Feature(12)631..633(+)
Misc Feature(13)727..744(+)
Misc Feature(14)754..756(+)
Exon (1)1..129
Gene:UCHL1
Gene Synonym:
Exon (2)130..141
Gene:UCHL1
Gene Synonym:
Exon (3)142..270
Gene:UCHL1
Gene Synonym:
Exon (4)271..421
Gene:UCHL1
Gene Synonym:
Exon (5)422..507
Gene:UCHL1
Gene Synonym:
Exon (6)508..555
Gene:UCHL1
Gene Synonym:
Exon (7)556..622
Gene:UCHL1
Gene Synonym:
Exon (8)623..681
Gene:UCHL1
Gene Synonym:
Exon (9)682..1124
Gene:UCHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004181
Organism Homo sapiens (human)
Definition Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004181

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCAGCTCA AGCCGATGGA GATCAACCCC GAGATGCTGA ACAAAGTGCT GTCCCGGCTG 
GGGGTCGCCG GCCAGTGGCG CTTCGTGGAC GTGCTGGGGC TGGAAGAGGA GTCTCTGGGC
TCGGTGCCAG CGCCTGCCTG CGCGCTGCTG CTGCTGTTTC CCCTCACGGC CCAGCATGAG
AACTTCAGGA AAAAGCAGAT TGAAGAGCTG AAGGGACAAG AAGTTAGTCC TAAAGTGTAC
TTCATGAAGC AGACCATTGG GAATTCCTGT GGCACAATCG GACTTATTCA CGCAGTGGCC
AATAATCAAG ACAAACTGGG ATTTGAGGAT GGATCAGTTC TGAAACAGTT TCTTTCTGAA
ACAGAGAAAA TGTCCCCTGA AGACAGAGCA AAATGCTTTG AAAAGAATGA GGCCATACAG
GCAGCCCATG ATGCCGTGGC ACAGGAAGGC CAATGTCGGG TAGATGACAA GGTGAATTTC
CATTTTATTC TGTTTAACAA CGTGGATGGC CACCTCTATG AACTTGATGG ACGAATGCCT
TTTCCGGTGA ACCATGGCGC CAGTTCAGAG GACACCCTGC TGAAGGACGC TGCCAAGGTC
TGCAGAGAAT TCACCGAGCG TGAGCAAGGA GAAGTCCGCT TCTCTGCCGT GGCTCTCTGC
AAGGCAGCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

KSHV LANA and EBV LMP1 induce the expression of UCH-L1 following viral transformation
Virology 448, 293-302 (2014)
Bentz GL, Bheda-Malge A, Wang L, Shackelford J, Damania B and Pagano JS.


book

Heterogeneous expression and functional relevance of the ubiquitin carboxyl-terminal hydrolase L1 in melanoma
Int. J. Cancer 133 (11), 2522-2532 (2013)
Wulfanger J, Biehl K, Tetzner A, Wild P, Ikenberg K, Meyer S and Seliger B.


book

Prognostic relevance of ubiquitin C-terminal hydrolase L1 (UCH-L1) mRNA and protein expression in breast cancer patients
J. Cancer Res. Clin. Oncol. 139 (10), 1745-1755 (2013)
Schroder C, Milde-Langosch K, Gebauer F, Schmid K, Mueller V, Wirtz RM, Meyer-Schwesinger C, Schluter H, Sauter G and Schumacher U.


book

Expression of neuronal markers in the endometrium of women with and those without endometriosis
Hum. Reprod. 28 (9), 2502-2510 (2013)
Newman TA, Bailey JL, Stocker LJ, Woo YL, Macklon NS and Cheong YC.


book

The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis
Cell Commun. Signal 11, 76 (2013)
Sosna J, Voigt S, Mathieu S, Kabelitz D, Trad A, Janssen O, Meyer-Schwesinger C, Schutze S and Adam D.


book

Parkinson Disease Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Farlow,J., Pankratz,N.D., Wojcieszek,J. and Foroud,T.


book

Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes
Electrophoresis 13 (12), 960-969 (1992)
Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE and Vandekerckhove J.


book

The gene for human neurone specific ubiquitin C-terminal hydrolase (UCHL1, PGP9.5) maps to chromosome 4p14
Ann. Hum. Genet. 55 (PT 4), 273-278 (1991)
Edwards YH, Fox MF, Povey S, Hinks LJ, Thompson RJ and Day IN.


book

Neuronal protein gene product 9.5 (IEF SSP 6104) is expressed in cultured human MRC-5 fibroblasts of normal origin and is strongly down-regulated in their SV40 transformed counterparts
FEBS Lett. 280 (2), 235-240 (1991)
Honore B, Rasmussen HH, Vandekerckhove J and Celis JE.


book

The structure of the human gene encoding protein gene product 9.5 (PGP9.5), a neuron-specific ubiquitin C-terminal hydrolase
Biochem. J. 268 (2), 521-524 (1990)
Day IN, Hinks LJ and Thompson RJ.


 
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