VARS1 cDNA ORF clone, Homo sapiens(human)

The following VARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu61296 XM_024446537.1
Latest version!
Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu61296 XM_017011246.1
Latest version!
Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu61296 XM_017011247.1
Latest version!
Homo sapiens valyl-tRNA synthetase (VARS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu19378 NM_006295.3
Latest version!
Homo sapiens valyl-tRNA synthetase 1 (VARS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu40453 XM_005249362.2
Latest version!
Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu19378 NM_006295.2 Homo sapiens valyl-tRNA synthetase (VARS), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu61296
    Clone ID Related Accession (Same CDS sequence) XM_017011246.1 , XM_017011247.1 , XM_024446537.1
    Accession Version XM_017011246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product valine--tRNA ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
    GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC
    AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG
    GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT
    TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC
    GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT
    GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC
    AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG
    GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG
    GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA
    GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC
    TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT
    CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC
    AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG
    ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG
    CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC
    AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC
    CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG
    ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT
    GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG
    GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC
    CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC
    TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT
    GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC
    TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT
    CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC
    GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG
    GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC
    ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG
    GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT
    GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC
    AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT
    GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG
    CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG
    ATGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866735.1
    CDS911..2959
    Translation

    Target ORF information:

    RefSeq Version XM_017011246.1
    Organism Homo sapiens(human)
    Definition Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
    GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC
    AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG
    GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT
    TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC
    GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT
    GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC
    AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG
    GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG
    GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA
    GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC
    TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT
    CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC
    AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG
    ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG
    CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC
    AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC
    CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG
    ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT
    GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG
    GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC
    CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC
    TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT
    GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC
    TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT
    CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC
    GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG
    GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC
    ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG
    GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT
    GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC
    AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT
    GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG
    CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG
    ATGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu61296
    Clone ID Related Accession (Same CDS sequence) XM_017011246.1 , XM_017011247.1 , XM_024446537.1
    Accession Version XM_017011247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product valine--tRNA ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
    GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC
    AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG
    GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT
    TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC
    GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT
    GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC
    AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG
    GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG
    GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA
    GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC
    TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT
    CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC
    AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG
    ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG
    CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC
    AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC
    CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG
    ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT
    GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG
    GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC
    CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC
    TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT
    GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC
    TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT
    CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC
    GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG
    GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC
    ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG
    GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT
    GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC
    AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT
    GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG
    CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG
    ATGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866736.1
    CDS914..2962
    Translation

    Target ORF information:

    RefSeq Version XM_017011247.1
    Organism Homo sapiens(human)
    Definition Homo sapiens valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
    GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC
    AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG
    GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT
    TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC
    GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT
    GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC
    AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG
    GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG
    GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA
    GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC
    TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT
    CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC
    AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG
    ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG
    CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC
    AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC
    CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG
    ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT
    GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG
    GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC
    CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC
    TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT
    GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC
    TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT
    CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC
    GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG
    GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC
    ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG
    GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT
    GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC
    AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT
    GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG
    CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG
    ATGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu61296
    Clone ID Related Accession (Same CDS sequence) XM_017011246.1 , XM_017011247.1 , XM_024446537.1
    Accession Version XM_024446537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product valine--tRNA ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
    GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC
    AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG
    GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT
    TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC
    GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT
    GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC
    AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG
    GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG
    GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA
    GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC
    TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT
    CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC
    AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG
    ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG
    CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC
    AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC
    CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG
    ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT
    GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG
    GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC
    CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC
    TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT
    GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC
    TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT
    CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC
    GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG
    GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC
    ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG
    GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT
    GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC
    AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT
    GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG
    CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG
    ATGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024302305.1
    CDS1025..3073
    Translation

    Target ORF information:

    RefSeq Version XM_024446537.1
    Organism Homo sapiens(human)
    Definition Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
    GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC
    AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG
    GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT
    TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC
    GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT
    GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC
    AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG
    GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG
    GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA
    GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC
    TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT
    CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC
    AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG
    ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG
    CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC
    AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC
    CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG
    ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT
    GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG
    GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC
    CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC
    TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT
    GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC
    TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT
    CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC
    GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG
    GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC
    ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG
    GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT
    GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC
    AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT
    GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG
    CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG
    ATGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19378
    Clone ID Related Accession (Same CDS sequence) NM_006295.2 , NM_006295.3
    Accession Version NM_006295.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-10
    Organism Homo sapiens(human)
    Product valine--tRNA ligase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX781162.1, CX167000.1, BC012808.2 and M98326.1. This sequence is a reference standard in the RefSeqGene project. On May 4, 2006 this sequence version replaced NM_006295.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59303.1, BC012808.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
    GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC
    TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG
    GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG
    CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC
    AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA
    CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT
    GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC
    CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC
    CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA
    GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA
    GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT
    GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC
    AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG
    CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG
    GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG
    GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC
    CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG
    CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT
    AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA
    ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG
    GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT
    GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC
    TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC
    CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG
    CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG
    CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT
    CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC
    CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC
    ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC
    CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG
    GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC
    CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT
    TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG
    ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC
    ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC
    CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC
    TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG
    AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT
    GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG
    AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC
    CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA
    GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG
    GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG
    CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC
    CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT
    GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA
    GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG
    GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG
    CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA
    CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA
    GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006286.1
    CDS424..4218
    Translation

    Target ORF information:

    RefSeq Version NM_006295.2
    Organism Homo sapiens(human)
    Definition Homo sapiens valyl-tRNA synthetase (VARS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006295.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
    GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC
    TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG
    GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG
    CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC
    AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA
    CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT
    GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC
    CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC
    CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA
    GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA
    GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT
    GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC
    AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG
    CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG
    GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG
    GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC
    CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG
    CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT
    AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA
    ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG
    GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT
    GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC
    TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC
    CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG
    CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG
    CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT
    CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC
    CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC
    ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC
    CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG
    GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC
    CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT
    TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG
    ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC
    ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC
    CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC
    TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG
    AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT
    GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG
    AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC
    CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA
    GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG
    GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG
    CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC
    CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT
    GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA
    GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG
    GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG
    CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA
    CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA
    GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19378
    Clone ID Related Accession (Same CDS sequence) NM_006295.2 , NM_006295.3
    Accession Version NM_006295.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Homo sapiens(human)
    Product valine--tRNA ligase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX781162.1, CX167000.1, BC012808.2 and M98326.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006295.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59303.1, BC012808.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000375663.8/ ENSP00000364815.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
    GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC
    TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG
    GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG
    CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC
    AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA
    CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT
    GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC
    CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC
    CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA
    GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA
    GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT
    GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC
    AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG
    CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG
    GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG
    GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC
    CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG
    CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT
    AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA
    ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG
    GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT
    GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC
    TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC
    CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG
    CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG
    CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT
    CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC
    CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC
    ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC
    CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG
    GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC
    CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT
    TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG
    ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC
    ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC
    CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC
    TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG
    AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT
    GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG
    AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC
    CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA
    GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG
    GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG
    CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC
    CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT
    GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA
    GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG
    GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG
    CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA
    CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA
    GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006286.1
    CDS241..4035
    Translation

    Target ORF information:

    RefSeq Version NM_006295.3
    Organism Homo sapiens(human)
    Definition Homo sapiens valyl-tRNA synthetase 1 (VARS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006295.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
    GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC
    TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG
    GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG
    CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC
    AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA
    CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT
    GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC
    CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC
    CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA
    GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA
    GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT
    GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC
    AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG
    CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC
    CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG
    GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG
    GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG
    GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC
    CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG
    CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT
    AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA
    ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG
    GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT
    GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC
    TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC
    CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG
    CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG
    CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT
    CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC
    CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC
    ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC
    CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG
    GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC
    CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT
    TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG
    ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC
    ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC
    CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC
    TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG
    AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT
    GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG
    AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC
    CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA
    GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG
    GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC
    TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG
    CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC
    CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT
    GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA
    GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG
    GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG
    CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA
    CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA
    GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40453
    Clone ID Related Accession (Same CDS sequence) XM_005249362.2
    Accession Version XM_005249362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product valine--tRNA ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005249362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
    GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC
    TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG
    GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG
    CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC
    AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA
    CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT
    GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC
    CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC
    CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA
    GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA
    GGGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGAGAAAC GGGATCCTGG GGTCATTACC
    TATGACCTCC CAACCCCACC CGGGGAAAAG AAAGATGTCA GTGGCCCCAT GCCCGACTCC
    TACAGCCCTC GGTATGTGGA GGCTGCCTGG TACCCTTGGT GGGAGCAGCA GGGCTTCTTC
    AAGCCAGAGT ATGGGCGTCC TAATGTGTCA GCAGCAAATC CCCGAGGTGT CTTCATGATG
    TGCATCCCAC CCCCCAATGT GACAGGCTCC CTGCACCTGG GCCATGCACT CACCAACGCC
    ATCCAGGACT CCCTGACTCG ATGGCACCGC ATGCGTGGGG AGACCACCCT GTGGAACCCT
    GGCTGTGACC ATGCAGGTAT TGCCACCCAG GTGGTGGTGG AGAAGAAGCT ATGGCGTGAG
    CAGGGACTGA GCCGGCACCA GCTGGGCCGC GAGGCCTTTC TACAGGAAGT CTGGAAGTGG
    AAGGAGGAGA AAGGTGACCG GATTTACCAC CAGTTGAAGA AGCTTGGCAG CTCCTTGGAC
    TGGGATCGAG CCTGTTTCAC CATGGACCCT AAACTCTCAG CAGCTGTGAC AGAGGCCTTT
    GTCCGGCTTC ACGAGGAAGG CATCATCTAT CGCAGTACCC GCCTTGTTAA CTGGTCCTGC
    ACCCTCAACT CCGCCATCTC TGACATTGAG GTGGATAAGA AGGAGCTGAC AGGTCGCACC
    CTGCTCTCCG TGCCTGGCTA CAAGGAGAAG GTGGAGTTCG GGGTCCTCGT GTCCTTTGCC
    TATAAGGTCC AAGGCTCAGA TAGCGACGAG GAGGTGGTGG TGGCAACAAC TCGGATCGAG
    ACAATGCTGG GAGATGTGGC TGTAGCTGTG CACCCCAAAG ATACCAGATA CCAGCACCTG
    AAGGGGAAGA ACGTGATCCA CCCATTCCTG TCTCGGAGCC TTCCCATTGT CTTCGATGAA
    TTTGTGGACA TGGACTTTGG CACAGGTGCT GTGAAGATCA CCCCCGCACA TGACCAAAAT
    GACTATGAAG TTGGGCAGCG GCACGGGCTG GAGGCCATCA GCATCATGGA CTCCCGGGGG
    GCCCTCATCA ATGTGCCTCC GCCTTTCCTG GGCCTGCCCA GGTTTGAGGC CAGGAAAGCG
    GTGCTGGTGG CGCTGAAGGA GCGGGGACTG TTCCGTGGCA TTGAGGACAA CCCCATGGTG
    GTGCCACTTT GCAACCGGTC GAAGGACGTG GTAGAGCCTC TGCTGCGGCC GCAGTGGTAC
    GTTCGCTGCG GGGAGATGGC CCAGGCTGCC AGCGCCGCTG TGACTCGGGG TGACCTCCGC
    ATCCTGCCTG AGGCCCATCA GCGCACATGG CATGCCTGGA TGGACAACAT CCGGGAGTGG
    TGCATTTCCA GGCAGCTGTG GTGGGGCCAT CGCATCCCAG CCTACTTTGT CACTGTCAGT
    GACCCAGCGG TGCCCCCTGG GGAGGACCCT GATGGGCGGT ACTGGGTGAG TGGACGCAAT
    GAGGCGGAGG CCCGGGAGAA GGCAGCCAAG GAGTTCGGAG TGTCCCCTGA CAAGATCAGT
    CTCCAGCAAG ATGAGGATGT ATTGGATACC TGGTTCTCCT CTGGCCTCTT CCCCTTATCC
    ATTTTGGGCT GGCCCAACCA GTCAGAAGAC CTGAGTGTGT TCTACCCCGG GACACTGCTG
    GAGACCGGTC ATGACATCCT CTTCTTCTGG GTGGCCCGGA TGGTCATGCT GGGCCTGAAG
    CTCACGGGCA GGCTGCCCTT TAGAGAGGTC TACCTCCATG CCATCGTGCG AGATGCTCAC
    GGCCGGAAGA TGAGCAAGTC TCTAGGCAAT GTCATCGATC CCCTGGACGT CATCTATGGA
    ATCTCCCTGC AGGGCCTCCA CAACCAGCTG CTGAACAGCA ACCTGGATCC CAGCGAGGTG
    GAGAAGGCCA AAGAAGGGCA GAAAGCTGAC TTCCCAGCGG GGATTCCTGA ATGTGGCACC
    GATGCTCTCC GGTTTGGATT ATGTGCCTAC ATGTCCCAGG GTCGTGACAT CAACCTGGAT
    GTGAACCGGA TACTGGGTTA CCGCCACTTC TGCAACAAGC TCTGGAATGC CACCAAGTTT
    GCCCTTCGTG GCCTTGGGAA GGGTTTTGTG CCCTCACCCA CCTCCCAGCC CGGAGGCCAT
    GAGAGCCTGG TGGACCGCTG GATCCGCAGC CGCCTGACAG AGGCTGTGAG GCTCAGCAAT
    CAAGGCTTCC AGGCCTACGA CTTCCCGGCC GTCACCACTG CCCAGTACAG CTTCTGGCTC
    TATGAGCTCT GTGATGTCTA CTTGGAGTGC CTGAAACCTG TACTGAATGG GGTGGACCAG
    GTGGCAGCTG AGTGTGCCCG CCAGACCCTG TACACTTGCC TGGACGTTGG CCTGCGGCTG
    CTCTCACCCT TCATGCCCTT CGTGACGGAG GAGCTGTTCC AGAGGCTGCC CCGGAGGATG
    CCGCAAGCTC CCCCTAGCCT CTGTGTTACC CCCTACCCGG AGCCCTCAGA GTGCTCCTGG
    AAGGACCCCG AGGCAGAAGC CGCCCTTGAG CTGGCGCTAA GCATCACGCG AGCCGTGCGC
    TCCCTGCGGG CCGACTACAA CCTCACCCGG ATCCGGCCTG ACTGTTTCCT GGAAGTGGCG
    GATGAGGCCA CGGGCGCCCT GGCATCGGCG GTGTCGGGCT ACGTGCAGGC CCTGGCCAGC
    GCAGGTGTGG TGGCTGTTCT GGCCCTGGGG GCTCCCGCCC CCCAGGGTTG CGCTGTGGCT
    CTGGCTTCTG ATCGCTGCTC CATCCACCTG CAGCTTCAGG GGCTGGTGGA CCCTGCACGG
    GAGCTGGGCA AGCTGCAAGC CAAGCGAGTT GAGGCCCAGC GGCAGGCCCA GCGTCTGCGG
    GAACGCCGTG CTGCCTCGGG CTATCCTGTC AAGGTGCCGC TCGAAGTCCA GGAGGCAGAT
    GAAGCCAAGC TCCAACAGAC AGAAGCAGAG CTCAGGAAGG TGGATGAGGC CATCGCCCTA
    TTCCAGAAGA TGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005249419.1
    CDS417..4214
    Translation

    Target ORF information:

    RefSeq Version XM_005249362.2
    Organism Homo sapiens(human)
    Definition Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
    GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC
    TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG
    GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG
    CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC
    AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA
    CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT
    GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC
    CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC
    CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA
    GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA
    GGGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGAGAAAC GGGATCCTGG GGTCATTACC
    TATGACCTCC CAACCCCACC CGGGGAAAAG AAAGATGTCA GTGGCCCCAT GCCCGACTCC
    TACAGCCCTC GGTATGTGGA GGCTGCCTGG TACCCTTGGT GGGAGCAGCA GGGCTTCTTC
    AAGCCAGAGT ATGGGCGTCC TAATGTGTCA GCAGCAAATC CCCGAGGTGT CTTCATGATG
    TGCATCCCAC CCCCCAATGT GACAGGCTCC CTGCACCTGG GCCATGCACT CACCAACGCC
    ATCCAGGACT CCCTGACTCG ATGGCACCGC ATGCGTGGGG AGACCACCCT GTGGAACCCT
    GGCTGTGACC ATGCAGGTAT TGCCACCCAG GTGGTGGTGG AGAAGAAGCT ATGGCGTGAG
    CAGGGACTGA GCCGGCACCA GCTGGGCCGC GAGGCCTTTC TACAGGAAGT CTGGAAGTGG
    AAGGAGGAGA AAGGTGACCG GATTTACCAC CAGTTGAAGA AGCTTGGCAG CTCCTTGGAC
    TGGGATCGAG CCTGTTTCAC CATGGACCCT AAACTCTCAG CAGCTGTGAC AGAGGCCTTT
    GTCCGGCTTC ACGAGGAAGG CATCATCTAT CGCAGTACCC GCCTTGTTAA CTGGTCCTGC
    ACCCTCAACT CCGCCATCTC TGACATTGAG GTGGATAAGA AGGAGCTGAC AGGTCGCACC
    CTGCTCTCCG TGCCTGGCTA CAAGGAGAAG GTGGAGTTCG GGGTCCTCGT GTCCTTTGCC
    TATAAGGTCC AAGGCTCAGA TAGCGACGAG GAGGTGGTGG TGGCAACAAC TCGGATCGAG
    ACAATGCTGG GAGATGTGGC TGTAGCTGTG CACCCCAAAG ATACCAGATA CCAGCACCTG
    AAGGGGAAGA ACGTGATCCA CCCATTCCTG TCTCGGAGCC TTCCCATTGT CTTCGATGAA
    TTTGTGGACA TGGACTTTGG CACAGGTGCT GTGAAGATCA CCCCCGCACA TGACCAAAAT
    GACTATGAAG TTGGGCAGCG GCACGGGCTG GAGGCCATCA GCATCATGGA CTCCCGGGGG
    GCCCTCATCA ATGTGCCTCC GCCTTTCCTG GGCCTGCCCA GGTTTGAGGC CAGGAAAGCG
    GTGCTGGTGG CGCTGAAGGA GCGGGGACTG TTCCGTGGCA TTGAGGACAA CCCCATGGTG
    GTGCCACTTT GCAACCGGTC GAAGGACGTG GTAGAGCCTC TGCTGCGGCC GCAGTGGTAC
    GTTCGCTGCG GGGAGATGGC CCAGGCTGCC AGCGCCGCTG TGACTCGGGG TGACCTCCGC
    ATCCTGCCTG AGGCCCATCA GCGCACATGG CATGCCTGGA TGGACAACAT CCGGGAGTGG
    TGCATTTCCA GGCAGCTGTG GTGGGGCCAT CGCATCCCAG CCTACTTTGT CACTGTCAGT
    GACCCAGCGG TGCCCCCTGG GGAGGACCCT GATGGGCGGT ACTGGGTGAG TGGACGCAAT
    GAGGCGGAGG CCCGGGAGAA GGCAGCCAAG GAGTTCGGAG TGTCCCCTGA CAAGATCAGT
    CTCCAGCAAG ATGAGGATGT ATTGGATACC TGGTTCTCCT CTGGCCTCTT CCCCTTATCC
    ATTTTGGGCT GGCCCAACCA GTCAGAAGAC CTGAGTGTGT TCTACCCCGG GACACTGCTG
    GAGACCGGTC ATGACATCCT CTTCTTCTGG GTGGCCCGGA TGGTCATGCT GGGCCTGAAG
    CTCACGGGCA GGCTGCCCTT TAGAGAGGTC TACCTCCATG CCATCGTGCG AGATGCTCAC
    GGCCGGAAGA TGAGCAAGTC TCTAGGCAAT GTCATCGATC CCCTGGACGT CATCTATGGA
    ATCTCCCTGC AGGGCCTCCA CAACCAGCTG CTGAACAGCA ACCTGGATCC CAGCGAGGTG
    GAGAAGGCCA AAGAAGGGCA GAAAGCTGAC TTCCCAGCGG GGATTCCTGA ATGTGGCACC
    GATGCTCTCC GGTTTGGATT ATGTGCCTAC ATGTCCCAGG GTCGTGACAT CAACCTGGAT
    GTGAACCGGA TACTGGGTTA CCGCCACTTC TGCAACAAGC TCTGGAATGC CACCAAGTTT
    GCCCTTCGTG GCCTTGGGAA GGGTTTTGTG CCCTCACCCA CCTCCCAGCC CGGAGGCCAT
    GAGAGCCTGG TGGACCGCTG GATCCGCAGC CGCCTGACAG AGGCTGTGAG GCTCAGCAAT
    CAAGGCTTCC AGGCCTACGA CTTCCCGGCC GTCACCACTG CCCAGTACAG CTTCTGGCTC
    TATGAGCTCT GTGATGTCTA CTTGGAGTGC CTGAAACCTG TACTGAATGG GGTGGACCAG
    GTGGCAGCTG AGTGTGCCCG CCAGACCCTG TACACTTGCC TGGACGTTGG CCTGCGGCTG
    CTCTCACCCT TCATGCCCTT CGTGACGGAG GAGCTGTTCC AGAGGCTGCC CCGGAGGATG
    CCGCAAGCTC CCCCTAGCCT CTGTGTTACC CCCTACCCGG AGCCCTCAGA GTGCTCCTGG
    AAGGACCCCG AGGCAGAAGC CGCCCTTGAG CTGGCGCTAA GCATCACGCG AGCCGTGCGC
    TCCCTGCGGG CCGACTACAA CCTCACCCGG ATCCGGCCTG ACTGTTTCCT GGAAGTGGCG
    GATGAGGCCA CGGGCGCCCT GGCATCGGCG GTGTCGGGCT ACGTGCAGGC CCTGGCCAGC
    GCAGGTGTGG TGGCTGTTCT GGCCCTGGGG GCTCCCGCCC CCCAGGGTTG CGCTGTGGCT
    CTGGCTTCTG ATCGCTGCTC CATCCACCTG CAGCTTCAGG GGCTGGTGGA CCCTGCACGG
    GAGCTGGGCA AGCTGCAAGC CAAGCGAGTT GAGGCCCAGC GGCAGGCCCA GCGTCTGCGG
    GAACGCCGTG CTGCCTCGGG CTATCCTGTC AAGGTGCCGC TCGAAGTCCA GGAGGCAGAT
    GAAGCCAAGC TCCAACAGAC AGAAGCAGAG CTCAGGAAGG TGGATGAGGC CATCGCCCTA
    TTCCAGAAGA TGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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