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VPREB1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol VPREB1
Entrez Gene ID 7441
Full Name pre-B lymphocyte 1
Synonyms CD179a, IGI, IGVPB, VPREB
General protein information
Preferred Names
immunoglobulin iota chain
Names
immunoglobulin iota chain
CD179a
v(pre)B protein
CD179 antigen-like family member A
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q11.22

Summary The protein encoded by this gene belongs to the immunoglobulin superfamily and is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. This gene encodes the iota polypeptide chain that is associated with the Ig-mu chain to form a molecular complex which is expressed on the surface of pre-B cells. The complex is thought to regulate Ig gene rearrangements in the early steps of B-cell differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]. lac of sum
Disorder MIM:

605141


mRNA and Protein(s)

mRNA Protein Name
NM_007128 NP_009059 immunoglobulin iota chain isoform 1 precursor
NM_001303509 NP_001290438 immunoglobulin iota chain isoform 2


Homo sapiens (human) VPREB1 NP_009059.1
Pan troglodytes (chimpanzee) VPREB1 XP_515010.2
Macaca mulatta (Rhesus monkey) VPREB1 XP_001089831.1
Canis lupus familiaris (dog) VPREB1 XP_543537.2
Mus musculus (house mouse) Vpreb2 NP_058679.1
Mus musculus (house mouse) Vpreb1 NP_058678.1
Rattus norvegicus (Norway rat) Vpreb1 NP_001102315.1
Rattus norvegicus (Norway rat) Vpreb2 NP_001128260.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VPREB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPREB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18799
NM_007128 Homo sapiens pre-B lymphocyte 1 (VPREB1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu61315 NM_001303509 Homo sapiens pre-B lymphocyte 1 (VPREB1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18799
Clone ID Related Accession (Same CDS sequence) NM_007128
Accession Version NM_007128.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product immunoglobulin iota chain isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG502270.1, BC017922.1 and AC245060.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2015 this sequence version replaced gi:18379350. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG531255.1, BC017922.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGTCCTGGG CTCCTGTCCT GCTCATGCTG TTTGTCTACT GCACAGGTTG TGGTCCTCAG 
CCGGTGCTGC ATCAGCCGCC GGCCATGTCC TCGGCCCTTG GAACCACAAT CCGCCTCACC
TGCACCCTGA GGAACGACCA TGACATCGGT GTGTACAGCG TCTACTGGTA CCAGCAGAGG
CCGGGCCACC CTCCCAGGTT CCTGCTGAGA TATTTCTCAC AATCAGACAA GAGCCAGGGC
CCCCAGGTCC CCCCTCGCTT CTCTGGATCC AAAGATGTGG CCAGGAACAG GGGGTATTTG
AGCATCTCTG AGCTGCAGCC TGAGGACGAG GCTATGTATT ACTGTGCTAT GGGGGCCCGC
AGCTCGGAGA AGGAGGAGAG GGAGAGGGAG TGGGAGGAAG AAATGGAACC CACTGCAGCC
AGGACACGTG TCCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009059.1
CDS35..472
Misc Feature(1)10..10(+)
Misc Feature(2)92..157(+)
Misc Feature(3)110..382(+)
Misc Feature(4)122..388(+)
Misc Feature(5)158..202(+)
Misc Feature(6)203..244(+)
Misc Feature(7)245..277(+)
Misc Feature(8)278..379(+)
Exon (1)1..80
Gene:VPREB1
Gene Synonym:
Exon (2)81..650
Gene:VPREB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007128
Organism Homo sapiens (human)
Definition Homo sapiens pre-B lymphocyte 1 (VPREB1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007128

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGTCCTGGG CTCCTGTCCT GCTCATGCTG TTTGTCTACT GCACAGGTTG TGGTCCTCAG 
CCGGTGCTGC ATCAGCCGCC GGCCATGTCC TCGGCCCTTG GAACCACAAT CCGCCTCACC
TGCACCCTGA GGAACGACCA TGACATCGGT GTGTACAGCG TCTACTGGTA CCAGCAGAGG
CCGGGCCACC CTCCCAGGTT CCTGCTGAGA TATTTCTCAC AATCAGACAA GAGCCAGGGC
CCCCAGGTCC CCCCTCGCTT CTCTGGATCC AAAGATGTGG CCAGGAACAG GGGGTATTTG
AGCATCTCTG AGCTGCAGCC TGAGGACGAG GCTATGTATT ACTGTGCTAT GGGGGCCCGC
AGCTCGGAGA AGGAGGAGAG GGAGAGGGAG TGGGAGGAAG AAATGGAACC CACTGCAGCC
AGGACACGTG TCCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61315
Clone ID Related Accession (Same CDS sequence) NM_001303509
Accession Version NM_001303509.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 435bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product immunoglobulin iota chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC245060.1. Transcript Variant: This variant (2) uses an alternate splice site in the coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG502270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529, SAMEA2162823 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGTCTGCAC CATGTCCTGG GCTCCTGTCC TGCTCATGCT GTTTGTCTAC TGCACAGCCG 
GTGCTGCATC AGCCGCCGGC CATGTCCTCG GCCCTTGGAA CCACAATCCG CCTCACCTGC
ACCCTGAGGA ACGACCATGA CATCGGTGTG TACAGCGTCT ACTGGTACCA GCAGAGGCCG
GGCCACCCTC CCAGGTTCCT GCTGAGATAT TTCTCACAAT CAGACAAGAG CCAGGGCCCC
CAGGTCCCCC CTCGCTTCTC TGGATCCAAA GATGTGGCCA GGAACAGGGG GTATTTGAGC
ATCTCTGAGC TGCAGCCTGA GGACGAGGCT ATGTATTACT GTGCTATGGG GGCCCGCAGC
TCGGAGAAGG AGGAGAGGGA GAGGGAGTGG GAGGAAGAAA TGGAACCCAC TGCAGCCAGG
ACACGTGTCC CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290438.1
CDS24..458
Misc Feature(1)10..10(+)
Misc Feature(2)96..368(+)
Misc Feature(3)108..374(+)
Exon (1)1..80
Gene:VPREB1
Gene Synonym:
Exon (2)81..636
Gene:VPREB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001303509
Organism Homo sapiens (human)
Definition Homo sapiens pre-B lymphocyte 1 (VPREB1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303509

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGTCTGCAC CATGTCCTGG GCTCCTGTCC TGCTCATGCT GTTTGTCTAC TGCACAGCCG 
GTGCTGCATC AGCCGCCGGC CATGTCCTCG GCCCTTGGAA CCACAATCCG CCTCACCTGC
ACCCTGAGGA ACGACCATGA CATCGGTGTG TACAGCGTCT ACTGGTACCA GCAGAGGCCG
GGCCACCCTC CCAGGTTCCT GCTGAGATAT TTCTCACAAT CAGACAAGAG CCAGGGCCCC
CAGGTCCCCC CTCGCTTCTC TGGATCCAAA GATGTGGCCA GGAACAGGGG GTATTTGAGC
ATCTCTGAGC TGCAGCCTGA GGACGAGGCT ATGTATTACT GTGCTATGGG GGCCCGCAGC
TCGGAGAAGG AGGAGAGGGA GAGGGAGTGG GAGGAAGAAA TGGAACCCAC TGCAGCCAGG
ACACGTGTCC CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

VPREB1 deletions occur independent of lambda light chain rearrangement in childhood acute lymphoblastic leukemia
Leukemia 28 (1), 216-220 (2014)
Mangum DS, Downie J, Mason CC, Jahromi MS, Joshi D, Rodic V, Muschen M, Meeker N, Trede N, Frazer JK, Zhou Y, Cheng C, Jeha S, Pui CH, Willman CL, Harvey RC, Hunger SP, Yang JJ, Barnette P, Mullighan CG, Miles RR and Schiffman JD.


book

The potential role of VPREB1 gene copy number variation in susceptibility to rheumatoid arthritis
Mol. Immunol. 48 (11), 1338-1343 (2011)
Yim SH, Chung YJ, Jin EH, Shim SC, Kim JY, Kim YS, Hu HJ, Shin SH, Pae HO, Zouali M and Chung HT.


book

Clustering of pre-B cell integrins induces galectin-1-dependent pre-B cell receptor relocalization and activation
J. Immunol. 177 (2), 796-803 (2006)
Rossi B, Espeli M, Schiff C and Gauthier L.


book

Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering
Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13014-13019 (2002)
Gauthier L, Rossi B, Roux F, Termine E and Schiff C.


book

The human V-preB promoter is a target for coordinated activation by early B cell factor and E47
J. Immunol. 168 (10), 5130-5138 (2002)
Gisler R and Sigvardsson M.


book

Conventional and surrogate light chains differentially regulate Ig mu and Dmu heavy chain maturation and surface expression
J. Immunol. 167 (7), 3846-3857 (2001)
Fang T, Smith BP and Roman CA.


book

The human pre-B-specific lambda-like cluster is located in the 22q11.2-22q12.3 region, distal to the IgC lambda locus
Genomics 9 (3), 544-546 (1991)
Mattei MG, Fumoux F, Roeckel N, Fougereau M and Schiff C.


book

The products of pre-B cell-specific genes (lambda 5 and VpreB) and the immunoglobulin mu chain form a complex that is transported onto the cell surface
J. Exp. Med. 172 (3), 973-976 (1990)
Tsubata T and Reth M.


book

Structure and pre-B lymphocyte restricted expression of the VpreB in humans and conservation of its structure in other mammalian species
EMBO J. 7 (1), 111-116 (1988)
Bauer SR, Kudo A and Melchers F.


book

The human Vpre B gene is located on chromosome 22 near a cluster of V lambda gene segments
Immunogenetics 28 (5), 328-333 (1988)
Bauer SR, Huebner K, Budarf M, Finan J, Erikson J, Emanuel BS, Nowell PC, Croce CM and Melchers F.


 
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