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WT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol WT1
Entrez Gene ID 7490
Full Name Wilms tumor 1
Synonyms AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33
General protein information
Preferred Names
Wilms tumor protein
Names
Wilms tumor protein
amino-terminal domain of EWS|last three zinc fingers of the DNA-binding domain of WT1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. lac of sum
Disorder MIM:

607102

Disorder Html: Wilms tumor, type 1, 194070 (3); Denys-Drash syndrome, 194080 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_000378 NP_000369 Wilms tumor protein isoform A
NM_001198551 NP_001185480 Wilms tumor protein isoform E
NM_024424 NP_077742 Wilms tumor protein isoform B
NM_001198552 NP_001185481 Wilms tumor protein isoform F
NM_024426 NP_077744 Wilms tumor protein isoform D



Homo sapiens (human) WT1 NP_077742.2
Pan troglodytes (chimpanzee) WT1 XP_001138640.3
Macaca mulatta (Rhesus monkey) WT1 XP_001084704.2
Canis lupus familiaris (dog) WT1 XP_005631206.1
Bos taurus (cattle) WT1 XP_003587050.2
Mus musculus (house mouse) Wt1 NP_659032.3
Rattus norvegicus (Norway rat) Wt1 NP_113722.2
Gallus gallus (chicken) WT1 NP_990547.1
Danio rerio (zebrafish) wt1a NP_571121.1
Xenopus (Silurana) tropicalis (western clawed frog) wt1 NP_001135625.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27056 NM_000378 Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $293.30
$419.00
OHu27316
NM_001198551 Homo sapiens Wilms tumor 1 (WT1), transcript variant E, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu27419 NM_024424 Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $300.30
$429.00
OHu27290 NM_001198552 Homo sapiens Wilms tumor 1 (WT1), transcript variant F, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu27351
NM_024426 Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$214.50-$300.30
$429.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27056
Clone ID Related Accession (Same CDS sequence) NM_000378
Accession Version NM_000378.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced gi:65507713. Transcript Variant: This variant (A) lacks an alternate in-frame exon and uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform A), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG CCACAGCACA
GGGTACGAGA GCGATAACCA CACAACGCCC ATCCTCTGCG GAGCCCAATA CAGAATACAC
ACGCACGGTG TCTTCAGAGG CATTCAGGAT GTGCGACGTG TGCCTGGAGT AGCCCCGACT
CTTGTACGGT CGGCATCTGA GACCAGTGAG AAACGCCCCT TCATGTGTGC TTACCCAGGC
TGCAATAAGA GATATTTTAA GCTGTCCCAC TTACAGATGC ACAGCAGGAA GCACACTGGT
GAGAAACCAT ACCAGTGTGA CTTCAAGGAC TGTGAACGAA GGTTTTCTCG TTCAGACCAG
CTCAAAAGAC ACCAAAGGAG ACATACAGGT GTGAAACCAT TCCAGTGTAA AACTTGTCAG
CGAAAGTTCT CCCGGTCCGA CCACCTGAAG ACCCACACCA GGACTCATAC AGGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000369.3
CDS191..1684
Misc Feature(1)395..1306(+)
Misc Feature(2)728..1657(+)
Misc Feature(3)1325..1384(+)
Misc Feature(4)1331..1384(+)
Misc Feature(5)1337..1555(+)
Misc Feature(6)1358..1441(+)
Misc Feature(7)1406..1474(+)
Misc Feature(8)1406..1471(+)
Misc Feature(9)1436..1438(+)
Misc Feature(10)1451..1525(+)
Misc Feature(11)1496..1558(+)
Misc Feature(12)1496..1558(+)
Misc Feature(13)1496..1558(+)
Misc Feature(14)1496..1558(+)
Misc Feature(15)1511..1645(+)
Misc Feature(16)1520..1522(+)
Misc Feature(17)1532..1615(+)
Misc Feature(18)1580..1648(+)
Misc Feature(19)1580..1648(+)
Misc Feature(20)395..397(+)
Misc Feature(21)1185..1185(+)
Exon (1)1..836
Gene:WT1
Gene Synonym:
Exon (2)837..959
Gene:WT1
Gene Synonym:
Exon (3)960..1062
Gene:WT1
Gene Synonym:
Exon (4)1063..1140
Gene:WT1
Gene Synonym:
Exon (5)1141..1237
Gene:WT1
Gene Synonym:
Exon (6)1238..1388
Gene:WT1
Gene Synonym:
Exon (7)1389..1478
Gene:WT1
Gene Synonym:
Exon (8)1479..1562
Gene:WT1
Gene Synonym:
Exon (9)1563..2967
Gene:WT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000378
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000378

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG CCACAGCACA
GGGTACGAGA GCGATAACCA CACAACGCCC ATCCTCTGCG GAGCCCAATA CAGAATACAC
ACGCACGGTG TCTTCAGAGG CATTCAGGAT GTGCGACGTG TGCCTGGAGT AGCCCCGACT
CTTGTACGGT CGGCATCTGA GACCAGTGAG AAACGCCCCT TCATGTGTGC TTACCCAGGC
TGCAATAAGA GATATTTTAA GCTGTCCCAC TTACAGATGC ACAGCAGGAA GCACACTGGT
GAGAAACCAT ACCAGTGTGA CTTCAAGGAC TGTGAACGAA GGTTTTCTCG TTCAGACCAG
CTCAAAAGAC ACCAAAGGAG ACATACAGGT GTGAAACCAT TCCAGTGTAA AACTTGTCAG
CGAAAGTTCT CCCGGTCCGA CCACCTGAAG ACCCACACCA GGACTCATAC AGGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27316
Clone ID Related Accession (Same CDS sequence) NM_001198551
Accession Version NM_001198551.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform E
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, BC032861.2, AK093168.1, M30393.1 and BC046461.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (E) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. The encoded isoform (E) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032861.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG
AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001185480.1
CDS279..1187
Misc Feature(1)231..233(+)
Misc Feature(2)285..809(+)
Misc Feature(3)783..>980(+)
Misc Feature(4)828..887(+)
Misc Feature(5)834..887(+)
Misc Feature(6)840..1058(+)
Misc Feature(7)861..944(+)
Misc Feature(8)909..977(+)
Misc Feature(9)909..974(+)
Misc Feature(10)954..1028(+)
Misc Feature(11)987..>1163(+)
Misc Feature(12)999..1061(+)
Misc Feature(13)999..1061(+)
Misc Feature(14)999..1061(+)
Misc Feature(15)999..1061(+)
Misc Feature(16)1014..1148(+)
Misc Feature(17)1035..1118(+)
Misc Feature(18)1083..1151(+)
Misc Feature(19)1083..1151(+)
Misc Feature(20)688..688(+)
Exon (1)1..288
Gene:WT1
Gene Synonym:
Exon (2)289..411
Gene:WT1
Gene Synonym:
Exon (3)412..514
Gene:WT1
Gene Synonym:
Exon (4)515..592
Gene:WT1
Gene Synonym:
Exon (5)593..643
Gene:WT1
Gene Synonym:
Exon (6)644..740
Gene:WT1
Gene Synonym:
Exon (7)741..891
Gene:WT1
Gene Synonym:
Exon (8)892..981
Gene:WT1
Gene Synonym:
Exon (9)982..1065
Gene:WT1
Gene Synonym:
Exon (10)1066..2470
Gene:WT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001198551
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001198551

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG
AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27419
Clone ID Related Accession (Same CDS sequence) NM_024424
Accession Version NM_024424.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced gi:65507816. Transcript Variant: This variant (B) uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform B), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTGAAAA GCCCTTCAGC
TGTCGGTGGC CAAGTTGTCA GAAAAAGTTT GCCCGGTCAG ATGAATTAGT CCGCCATCAC
AACATGCATC AGAGAAACAT GACCAAACTC CAGCTGGCGC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_077742.2
CDS191..1735
Misc Feature(1)395..1357(+)
Misc Feature(2)1346..1603(+)
Misc Feature(3)1376..1435(+)
Misc Feature(4)1382..1435(+)
Misc Feature(5)1388..1606(+)
Misc Feature(6)1409..1492(+)
Misc Feature(7)1457..1525(+)
Misc Feature(8)1457..1522(+)
Misc Feature(9)1487..1489(+)
Misc Feature(10)1502..1576(+)
Misc Feature(11)1535..>1711(+)
Misc Feature(12)1547..1609(+)
Misc Feature(13)1547..1609(+)
Misc Feature(14)1547..1609(+)
Misc Feature(15)1547..1609(+)
Misc Feature(16)1562..1696(+)
Misc Feature(17)1571..1573(+)
Misc Feature(18)1583..1666(+)
Misc Feature(19)1631..1699(+)
Misc Feature(20)1631..1699(+)
Misc Feature(21)395..397(+)
Misc Feature(22)1236..1236(+)
Exon (1)1..836
Gene:WT1
Gene Synonym:
Exon (2)837..959
Gene:WT1
Gene Synonym:
Exon (3)960..1062
Gene:WT1
Gene Synonym:
Exon (4)1063..1140
Gene:WT1
Gene Synonym:
Exon (5)1141..1191
Gene:WT1
Gene Synonym:
Exon (6)1192..1288
Gene:WT1
Gene Synonym:
Exon (7)1289..1439
Gene:WT1
Gene Synonym:
Exon (8)1440..1529
Gene:WT1
Gene Synonym:
Exon (9)1530..1613
Gene:WT1
Gene Synonym:
Exon (10)1614..3018
Gene:WT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_024424
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_024424

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTGAAAA GCCCTTCAGC
TGTCGGTGGC CAAGTTGTCA GAAAAAGTTT GCCCGGTCAG ATGAATTAGT CCGCCATCAC
AACATGCATC AGAGAAACAT GACCAAACTC CAGCTGGCGC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27290
Clone ID Related Accession (Same CDS sequence) NM_001198552
Accession Version NM_001198552.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform F
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, AK093168.1, M30393.1 and BC046461.1. Transcript Variant: This variant (F) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also lacks an alternate, in-frame exon compared to variant D. The encoded isoform (F) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001185481.1
CDS279..1145
Misc Feature(1)231..233(+)
Misc Feature(2)285..758(+)
Misc Feature(3)732..>1118(+)
Misc Feature(4)777..836(+)
Misc Feature(5)783..836(+)
Misc Feature(6)789..1007(+)
Misc Feature(7)810..893(+)
Misc Feature(8)858..926(+)
Misc Feature(9)858..923(+)
Misc Feature(10)903..977(+)
Misc Feature(11)948..1010(+)
Misc Feature(12)948..1010(+)
Misc Feature(13)948..1010(+)
Misc Feature(14)948..1010(+)
Misc Feature(15)963..1106(+)
Misc Feature(16)1041..1109(+)
Misc Feature(17)1041..1109(+)
Misc Feature(18)637..637(+)
Exon (1)1..288
Gene:WT1
Gene Synonym:
Exon (2)289..411
Gene:WT1
Gene Synonym:
Exon (3)412..514
Gene:WT1
Gene Synonym:
Exon (4)515..592
Gene:WT1
Gene Synonym:
Exon (5)593..689
Gene:WT1
Gene Synonym:
Exon (6)690..840
Gene:WT1
Gene Synonym:
Exon (7)841..930
Gene:WT1
Gene Synonym:
Exon (8)931..1023
Gene:WT1
Gene Synonym:
Exon (9)1024..2428
Gene:WT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001198552
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant F, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001198552

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27351
Clone ID Related Accession (Same CDS sequence) NM_024426
Accession Version NM_024426.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform D
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced gi:65508003. Transcript Variant: This variant (D) represents the longest transcript and encodes the longest isoform (D). It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51630.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_077744.3
CDS191..1744
Misc Feature(1)395..1357(+)
Misc Feature(2)1331..>1717(+)
Misc Feature(3)1376..1435(+)
Misc Feature(4)1382..1435(+)
Misc Feature(5)1388..1606(+)
Misc Feature(6)1409..1492(+)
Misc Feature(7)1457..1525(+)
Misc Feature(8)1457..1522(+)
Misc Feature(9)1487..1489(+)
Misc Feature(10)1502..1576(+)
Misc Feature(11)1547..1609(+)
Misc Feature(12)1547..1609(+)
Misc Feature(13)1547..1609(+)
Misc Feature(14)1547..1609(+)
Misc Feature(15)1562..1705(+)
Misc Feature(16)1571..1573(+)
Misc Feature(17)1640..1708(+)
Misc Feature(18)1640..1708(+)
Misc Feature(19)395..397(+)
Misc Feature(20)1236..1236(+)
Exon (1)1..836
Gene:WT1
Gene Synonym:
Exon (2)837..959
Gene:WT1
Gene Synonym:
Exon (3)960..1062
Gene:WT1
Gene Synonym:
Exon (4)1063..1140
Gene:WT1
Gene Synonym:
Exon (5)1141..1191
Gene:WT1
Gene Synonym:
Exon (6)1192..1288
Gene:WT1
Gene Synonym:
Exon (7)1289..1439
Gene:WT1
Gene Synonym:
Exon (8)1440..1529
Gene:WT1
Gene Synonym:
Exon (9)1530..1622
Gene:WT1
Gene Synonym:
Exon (10)1623..3027
Gene:WT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_024426
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_024426

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of a Novel C-Terminal Truncated WT1 Isoform with Antagonistic Effects against Major WT1 Isoforms
PLoS ONE 10 (6), E0130578 (2015)
Tatsumi N, Hojo N, Sakamoto H, Inaba R, Moriguchi N, Matsuno K, Fukuda M, Matsumura A, Hayashi S, Morimoto S, Nakata J, Fujiki F, Nishida S, Nakajima H, Tsuboi A, Oka Y, Hosen N, Sugiyama H and Oji Y.


book

A high incidence of WT1 abnormality in bilateral Wilms tumours in Japan, and the penetrance rates in children with WT1 germline mutation
Br. J. Cancer 112 (6), 1121-1133 (2015)
Kaneko Y, Okita H, Haruta M, Arai Y, Oue T, Tanaka Y, Horie H, Hinotsu S, Koshinaga T, Yoneda A, Ohtsuka Y, Taguchi T and Fukuzawa M.


book

The complex life of WT1
J. Cell. Sci. 116 (PT 9), 1653-1658 (2003)
Wagner KD, Wagner N and Schedl A.


book

Paternal expression of WT1 in human fibroblasts and lymphocytes
Hum. Mol. Genet. 6 (13), 2243-2246 (1997)
Mitsuya K, Sui H, Meguro M, Kugoh H, Jinno Y, Niikawa N and Oshimura M.


book

A non-AUG translational initiation event generates novel WT1 isoforms
J. Biol. Chem. 271 (15), 8646-8654 (1996)
Bruening W and Pelletier J.


book

RNA editing in the Wilms' tumor susceptibility gene, WT1
Genes Dev. 8 (6), 720-731 (1994)
Sharma PM, Bowman M, Madden SL, Rauscher FJ 3rd and Sukumar S.


book

Mosaic and polymorphic imprinting of the WT1 gene in humans
Nat. Genet. 6 (3), 305-309 (1994)
Jinno Y, Yun K, Nishiwaki K, Kubota T, Ogawa O, Reeve AE and Niikawa N.


book

Congenital Diaphragmatic Hernia Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Pober,B.R., Russell,M.K. and Ackerman,K.G.


book

Wilms Tumor Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Dome,J.S. and Huff,V.


book

Germline intronic and exonic mutations in the Wilms' tumour gene (WT1) affecting urogenital development
Nat. Genet. 1 (2), 144-148 (1992)
Bruening W, Bardeesy N, Silverman BL, Cohn RA, Machin GA, Aronson AJ, Housman D and Pelletier J.


 
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