CACNA1A cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CACNA1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25978 NM_023035.3
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$929.50-$1301.30
$1859.00
OHu21065 NM_001174080.2
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu25901 NM_000068.4
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu26122 NM_001127222.2
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu26360 NM_001127221.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
View Old Accession Versions >>
OHu26122 NM_001127222.1 Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu25901 NM_000068.3 Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu25978 NM_023035.2 Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$929.50-$1301.30
$1859.00
OHu21065 NM_001174080.1 Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25978
    Clone ID Related Accession (Same CDS sequence) NM_023035.2 , NM_023035.3
    Accession Version NM_023035.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF004884.1, BQ340521.1, AB035726.1 and AC011446.6. On Jun 1, 2007 this sequence version replaced NM_023035.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). This variant includes a (CAG)n-repeat in the coding region, resulting in a polyglutamine tract near the C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF004884.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGGG CAGTAGTTCC GTAAGTGGAA GCCCAGCCCC CTCAACATCT
    GGTACCAGCA CTCCGCGGCG GGGCCGCCGC CAGCTCCCCC AGACCCCCTC CACCCCCCGG
    CCACACGTGT CCTATTCCCC TGTGATCCGT AAGGCCGGCG GCTCGGGGCC CCCGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGGCGGTGG CCAGGCCGGG CCGGGCGGCC
    ACCAGCGGCC CTCGGAGGTA CCCAGGCCCC ACGGCCGAGC CTCTGGCCGG AGATCGGCCG
    CCCACGGGGG GCCACAGCAG CGGCCGCTCG CCCAGGATGG AGAGGCGGGT CCCAGGCCCG
    GCCCGGAGCG AGTCCCCCAG GGCCTGTCGA CACGGCGGGG CCCGGTGGCC GGCATCTGGC
    CCGCACGTGT CCGAGGGGCC CCCGGGTCCC CGGCACCATG GCTACTACCG GGGCTCCGAC
    TACGACGAGG CCGATGGCCC GGGCAGCGGG GGCGGCGAGG AGGCCATGGC CGGGGCCTAC
    GACGCGCCAC CCCCCGTACG ACACGCGTCC TCGGGCGCCA CCGGGCGCTC GCCCAGGACT
    CCCCGGGCCT CGGGCCCGGC CTGCGCCTCG CCTTCTCGGC ACGGCCGGCG ACTCCCCAAC
    GGCTACTACC CGGCGCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCAG GAAGGGCCTG
    CACGAACCCT ACAGCGAGAG TGACGATGAT TGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_075461.2
    CDS237..7775
    Translation

    Target ORF information:

    RefSeq Version NM_023035.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_023035.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGGG CAGTAGTTCC GTAAGTGGAA GCCCAGCCCC CTCAACATCT
    GGTACCAGCA CTCCGCGGCG GGGCCGCCGC CAGCTCCCCC AGACCCCCTC CACCCCCCGG
    CCACACGTGT CCTATTCCCC TGTGATCCGT AAGGCCGGCG GCTCGGGGCC CCCGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGGCGGTGG CCAGGCCGGG CCGGGCGGCC
    ACCAGCGGCC CTCGGAGGTA CCCAGGCCCC ACGGCCGAGC CTCTGGCCGG AGATCGGCCG
    CCCACGGGGG GCCACAGCAG CGGCCGCTCG CCCAGGATGG AGAGGCGGGT CCCAGGCCCG
    GCCCGGAGCG AGTCCCCCAG GGCCTGTCGA CACGGCGGGG CCCGGTGGCC GGCATCTGGC
    CCGCACGTGT CCGAGGGGCC CCCGGGTCCC CGGCACCATG GCTACTACCG GGGCTCCGAC
    TACGACGAGG CCGATGGCCC GGGCAGCGGG GGCGGCGAGG AGGCCATGGC CGGGGCCTAC
    GACGCGCCAC CCCCCGTACG ACACGCGTCC TCGGGCGCCA CCGGGCGCTC GCCCAGGACT
    CCCCGGGCCT CGGGCCCGGC CTGCGCCTCG CCTTCTCGGC ACGGCCGGCG ACTCCCCAAC
    GGCTACTACC CGGCGCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCAG GAAGGGCCTG
    CACGAACCCT ACAGCGAGAG TGACGATGAT TGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25978
    Clone ID Related Accession (Same CDS sequence) NM_023035.2 , NM_023035.3
    Accession Version NM_023035.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-30
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008540.6, AF004884.1, BQ340521.1, AB035726.1 and AC011446.6. On Jun 2, 2019 this sequence version replaced NM_023035.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). This variant includes a (CAG)n-repeat in the coding region, resulting in a polyglutamine tract near the C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF004884.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGGG CAGTAGTTCC GTAAGTGGAA GCCCAGCCCC CTCAACATCT
    GGTACCAGCA CTCCGCGGCG GGGCCGCCGC CAGCTCCCCC AGACCCCCTC CACCCCCCGG
    CCACACGTGT CCTATTCCCC TGTGATCCGT AAGGCCGGCG GCTCGGGGCC CCCGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGGCGGTGG CCAGGCCGGG CCGGGCGGCC
    ACCAGCGGCC CTCGGAGGTA CCCAGGCCCC ACGGCCGAGC CTCTGGCCGG AGATCGGCCG
    CCCACGGGGG GCCACAGCAG CGGCCGCTCG CCCAGGATGG AGAGGCGGGT CCCAGGCCCG
    GCCCGGAGCG AGTCCCCCAG GGCCTGTCGA CACGGCGGGG CCCGGTGGCC GGCATCTGGC
    CCGCACGTGT CCGAGGGGCC CCCGGGTCCC CGGCACCATG GCTACTACCG GGGCTCCGAC
    TACGACGAGG CCGATGGCCC GGGCAGCGGG GGCGGCGAGG AGGCCATGGC CGGGGCCTAC
    GACGCGCCAC CCCCCGTACG ACACGCGTCC TCGGGCGCCA CCGGGCGCTC GCCCAGGACT
    CCCCGGGCCT CGGGCCCGGC CTGCGCCTCG CCTTCTCGGC ACGGCCGGCG ACTCCCCAAC
    GGCTACTACC CGGCGCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCAG GAAGGGCCTG
    CACGAACCCT ACAGCGAGAG TGACGATGAT TGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_075461.2
    CDS256..7794
    Translation

    Target ORF information:

    RefSeq Version NM_023035.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_023035.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGGG CAGTAGTTCC GTAAGTGGAA GCCCAGCCCC CTCAACATCT
    GGTACCAGCA CTCCGCGGCG GGGCCGCCGC CAGCTCCCCC AGACCCCCTC CACCCCCCGG
    CCACACGTGT CCTATTCCCC TGTGATCCGT AAGGCCGGCG GCTCGGGGCC CCCGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGGCGGTGG CCAGGCCGGG CCGGGCGGCC
    ACCAGCGGCC CTCGGAGGTA CCCAGGCCCC ACGGCCGAGC CTCTGGCCGG AGATCGGCCG
    CCCACGGGGG GCCACAGCAG CGGCCGCTCG CCCAGGATGG AGAGGCGGGT CCCAGGCCCG
    GCCCGGAGCG AGTCCCCCAG GGCCTGTCGA CACGGCGGGG CCCGGTGGCC GGCATCTGGC
    CCGCACGTGT CCGAGGGGCC CCCGGGTCCC CGGCACCATG GCTACTACCG GGGCTCCGAC
    TACGACGAGG CCGATGGCCC GGGCAGCGGG GGCGGCGAGG AGGCCATGGC CGGGGCCTAC
    GACGCGCCAC CCCCCGTACG ACACGCGTCC TCGGGCGCCA CCGGGCGCTC GCCCAGGACT
    CCCCGGGCCT CGGGCCCGGC CTGCGCCTCG CCTTCTCGGC ACGGCCGGCG ACTCCCCAAC
    GGCTACTACC CGGCGCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCAG GAAGGGCCTG
    CACGAACCCT ACAGCGAGAG TGACGATGAT TGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21065
    Clone ID Related Accession (Same CDS sequence) NM_001174080.1 , NM_001174080.2
    Accession Version NM_001174080.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF004883.1, FJ040507.1 and AC011446.6. Transcript Variant: This variant (5) uses two alternate splice sites, compared to variant 2. The resulting isoform (5) lacks an internal 3-aa segment and has a shorter and distinct C-terminus that does not include the polyglutamine tract, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGACGA GCAAGAGGAA GAAGAAGCAG CGAACCAGAA ACTTGCCCTA
    CAGAAAGCCA AGGAGGTGGC AGAAGTGAGT CCTCTGTCCG CGGCCAACAT GTCTATAGCT
    GTGAAAGAGC AACAGAAGAA TCAAAAGCCA GCCAAGTCCG TGTGGGAGCA GCGGACCAGT
    GAGATGCGAA AGCAGAACTT GCTGGCCAGC CGGGAGGCCC TGTATAACGA AATGGACCCG
    GACGAGCGCT GGAAGGCTGC CTACACGCGG CACCTGCGGC CAGACATGAA GACGCACTTG
    GACCGGCCGC TGGTGGTGGA CCCGCAGGAG AACCGCAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACCGTGGA CCAGCGCCTC GGCCAGCAGC GCGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA TCGGGCCCGG GACCCCAGCG GCTCGGCGGG CCTGGACGCA
    CGGAGGCCCT GGGCGGGAAG CCAGGAGGCC GAGCTGAGCC GGGAGGGACC CTACGGCCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCAGC CTGGAGCAAC CCGGGTTCTG GGAGGGCGAG
    GCCGAGCGAG GCAAGGCCGG GGACCCCCAC CGGAGGCACG TGCACCGGCA GGGGGGCAGC
    AGGGAGAGCC GCAGCGGGTC CCCGCGCACG GGCGCGGACG GGGAGCATCG ACGTCATCGC
    GCGCACCGCA GGCCCGGGGA GGAGGGTCCG GAGGACAAGG CGGAGCGGAG GGCGCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGG GCGAGGGCCC CGACGGGGGC
    GAGCGCAGGA GAAGGCACCG GCATGGCGCT CCAGCCACGT ACGAGGGGGA CGCGCGGAGG
    GAGGACAAGG AGCGGAGGCA TCGGAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCTGTG
    TCGGGCCCCA ACCTGTCAAC CACCCGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCA
    CCCCTGGCAG AGGATATTGA CAACATGAAG AACAACAAGC TGGCCACCGC GGAGTCGGCC
    GCTCCCCACG GCAGCCTTGG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGAAAC
    AGCACCGACC CCGGCCCCAT GCTGGCCATC CCTGCCATGG CCACCAACCC CCAGAACGCC
    GCCAGCCGCC GGACGCCCAA CAACCCGGGG AACCCATCCA ATCCCGGCCC CCCCAAGACC
    CCCGAGAATA GCCTTATCGT CACCAACCCC AGCGGCACCC AGACCAATTC AGCTAAGACT
    GCCAGGAAAC CCGACCACAC CACAGTGGAC ATCCCCCCAG CCTGCCCACC CCCCCTCAAC
    CACACCGTCG TACAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAAGAGGAA
    GAGAAGAAGG AGGAGGAGGA AGACGACCGT GGGGAAGACG GCCCTAAGCC AATGCCTCCC
    TATAGCTCCA TGTTCATCCT GTCCACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATC CTCATGGTCA TTGCCATGAG CAGCATCGCC
    CTGGCCGCCG AGGACCCTGT GCAGCCCAAC GCACCTCGGA ACAACGTGCT GCGATACTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT TGACCTGGGG
    CTCGTCCTGC ATCAGGGTGC CTACTTCCGT GACCTCTGGA ATATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTAGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA AGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGCTG
    CCAAAGCTCA AGGCTGTGTT TGACTGTGTG GTGAACTCAC TTAAAAACGT CTTCAACATC
    CTCATCGTCT ACATGCTATT CATGTTCATC TTCGCCGTGG TGGCTGTGCA GCTCTTCAAG
    GGGAAATTCT TCCACTGCAC TGACGAGTCC AAAGAGTTTG AGAAAGATTG TCGAGGCAAA
    TACCTCCTCT ACGAGAAGAA TGAGGTGAAG GCGCGAGACC GGGAGTGGAA GAAGTATGAA
    TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCATTCGGTG GACGCCACCT TTGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATTTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ATATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AATACAGCCT GGAGAAAAAT GAGAGGGCCT GCATTGATTT CGCCATCAGC
    GCCAAGCCGC TGACCCGACA CATGCCGCAG AACAAGCAGA GCTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCTCCGCC TTTCGAGTAC ACGATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTTA TGATGAAGTT CTATGGGGCT TCTGTTGCTT ATGAAAATGC CCTGCGGGTG
    TTCAACATCG TCTTCACCTC CCTCTTCTCT CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTCCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ATATCCTCGT GACTGAGTTT GGGAATCCGA ATAACTTCAT CAACCTGAGC
    TTTCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTTC TCCGTCAGGG TTACACCATC
    CGCATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCTTATGT CTGTCTGCTG
    ATCGCCATGC TCTTCTTCAT CTATGCCATC ATTGGGATGC AGGTGTTTGG TAACATTGGC
    ATCGACGTGG AGGACGAGGA CAGTGATGAA GATGAGTTCC AAATCACTGA GCACAATAAC
    TTCCGGACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACCGG GGAAGCTTGG
    CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCGTGTG ATAAGAACTC TGGCATCCTG
    ACTCGAGAGT GTGGCAATGA ATTTGCTTAT TTTTACTTTG TTTCCTTCAT CTTCCTCTGC
    TCGTTTCTGA TGCTGAATCT CTTTGTCGCC GTCATCATGG ACAACTTTGA GTACCTCACC
    CGAGACTCCT CCATCCTGGG CCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG
    TATGACCCCG CAGCTTGGGG CCGCATGCCT TACCTGGACA TGTATCAGAT GCTGAGACAC
    ATGTCTCCGC CCCTGGGTCT GGGGAAGAAG TGTCCGGCCA GAGTGGCTTA CAAGCGGCTT
    CTGCGGATGG ACCTGCCCGT CGCAGATGAC AACACCGTCC ACTTCAATTC CACCCTCATG
    GCTCTGATCC GCACAGCCCT GGACATCAAG ATTGCCAAGG GAGGAGCCGA CAAACAGCAG
    ATGGACGCTG AGCTGCGGAA GGAGATGATG GCGATTTGGC CCAATCTGTC CCAGAAGACG
    CTAGACCTGC TGGTCACACC TCACAAGTCC ACGGACCTCA CCGTGGGGAA GATCTACGCA
    GCCATGATGA TCATGGAGTA CTACCGGCAG AGCAAGGCCA AGAAGCTGCA GGCCATGCGC
    GAGGAGCAGG ACCGGACACC CCTCATGTTC CAGCGCATGG AGCCCCCGTC CCCAACGCAG
    GAAGGGGGAC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGCCCTG
    ATGGCTCACG AAAGCGGCCT CAAGGAGAGC CCGTCCTGGG TGACCCAGCG TGCCCAGGAG
    ATGTTCCAGA AGACGGGCAC ATGGAGTCCG GAACAAGGCC CCCCTACCGA CATGCCCAAC
    AGCCAGCCTA ACTCTCAGTC CGTGGAGATG CGAGAGATGG GCAGAGATGG CTACTCCGAC
    AGCGAGCACT ACCTCCCCAT GGAAGGCCAG GGCCGGGCTG CCTCCATGCC CCGCCTCCCT
    GCAGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC
    ACCAGCCCCA TGAAGCGTTC AGCCTCCGTG CTGGGCCCCA AGGCCCGACG CCTGGACGAT
    TACTCGCTGG AGCGGGTCCC GCCCGAGGAG AACCAGCGGC ACCACCAGCG GCGCCGCGAC
    CGCAGCCACC GCGCCTCTGA GCGCTCCCTG GGCCGCTACA CCGATGTGGA CACAGGCTTG
    GGGACAGACC TGAGCATGAC CACCCAATCC GGGGACCTGC CGTCGAAGGA GCGGGACCAG
    GAGCGGGGCC GGCCCAAGGA TCGGAAGCAT CGACAGCACC ACCACCACCA CCACCACCAC
    CACCATCCCC CGCCCCCCGA CAAGGACCGC TATGCCCAGG AACGGCCGGA CCACGGCCGG
    GCACGGGCTC GGGACCAGCG CTGGTCCCGC TCGCCCAGCG AGGGCCGAGA GCACATGGCG
    CACCGGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001167551.1
    CDS237..7028
    Translation

    Target ORF information:

    RefSeq Version NM_001174080.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001174080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGACGA GCAAGAGGAA GAAGAAGCAG CGAACCAGAA ACTTGCCCTA
    CAGAAAGCCA AGGAGGTGGC AGAAGTGAGT CCTCTGTCCG CGGCCAACAT GTCTATAGCT
    GTGAAAGAGC AACAGAAGAA TCAAAAGCCA GCCAAGTCCG TGTGGGAGCA GCGGACCAGT
    GAGATGCGAA AGCAGAACTT GCTGGCCAGC CGGGAGGCCC TGTATAACGA AATGGACCCG
    GACGAGCGCT GGAAGGCTGC CTACACGCGG CACCTGCGGC CAGACATGAA GACGCACTTG
    GACCGGCCGC TGGTGGTGGA CCCGCAGGAG AACCGCAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACCGTGGA CCAGCGCCTC GGCCAGCAGC GCGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA TCGGGCCCGG GACCCCAGCG GCTCGGCGGG CCTGGACGCA
    CGGAGGCCCT GGGCGGGAAG CCAGGAGGCC GAGCTGAGCC GGGAGGGACC CTACGGCCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCAGC CTGGAGCAAC CCGGGTTCTG GGAGGGCGAG
    GCCGAGCGAG GCAAGGCCGG GGACCCCCAC CGGAGGCACG TGCACCGGCA GGGGGGCAGC
    AGGGAGAGCC GCAGCGGGTC CCCGCGCACG GGCGCGGACG GGGAGCATCG ACGTCATCGC
    GCGCACCGCA GGCCCGGGGA GGAGGGTCCG GAGGACAAGG CGGAGCGGAG GGCGCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGG GCGAGGGCCC CGACGGGGGC
    GAGCGCAGGA GAAGGCACCG GCATGGCGCT CCAGCCACGT ACGAGGGGGA CGCGCGGAGG
    GAGGACAAGG AGCGGAGGCA TCGGAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCTGTG
    TCGGGCCCCA ACCTGTCAAC CACCCGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCA
    CCCCTGGCAG AGGATATTGA CAACATGAAG AACAACAAGC TGGCCACCGC GGAGTCGGCC
    GCTCCCCACG GCAGCCTTGG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGAAAC
    AGCACCGACC CCGGCCCCAT GCTGGCCATC CCTGCCATGG CCACCAACCC CCAGAACGCC
    GCCAGCCGCC GGACGCCCAA CAACCCGGGG AACCCATCCA ATCCCGGCCC CCCCAAGACC
    CCCGAGAATA GCCTTATCGT CACCAACCCC AGCGGCACCC AGACCAATTC AGCTAAGACT
    GCCAGGAAAC CCGACCACAC CACAGTGGAC ATCCCCCCAG CCTGCCCACC CCCCCTCAAC
    CACACCGTCG TACAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAAGAGGAA
    GAGAAGAAGG AGGAGGAGGA AGACGACCGT GGGGAAGACG GCCCTAAGCC AATGCCTCCC
    TATAGCTCCA TGTTCATCCT GTCCACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATC CTCATGGTCA TTGCCATGAG CAGCATCGCC
    CTGGCCGCCG AGGACCCTGT GCAGCCCAAC GCACCTCGGA ACAACGTGCT GCGATACTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT TGACCTGGGG
    CTCGTCCTGC ATCAGGGTGC CTACTTCCGT GACCTCTGGA ATATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTAGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA AGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGCTG
    CCAAAGCTCA AGGCTGTGTT TGACTGTGTG GTGAACTCAC TTAAAAACGT CTTCAACATC
    CTCATCGTCT ACATGCTATT CATGTTCATC TTCGCCGTGG TGGCTGTGCA GCTCTTCAAG
    GGGAAATTCT TCCACTGCAC TGACGAGTCC AAAGAGTTTG AGAAAGATTG TCGAGGCAAA
    TACCTCCTCT ACGAGAAGAA TGAGGTGAAG GCGCGAGACC GGGAGTGGAA GAAGTATGAA
    TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCATTCGGTG GACGCCACCT TTGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATTTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ATATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AATACAGCCT GGAGAAAAAT GAGAGGGCCT GCATTGATTT CGCCATCAGC
    GCCAAGCCGC TGACCCGACA CATGCCGCAG AACAAGCAGA GCTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCTCCGCC TTTCGAGTAC ACGATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTTA TGATGAAGTT CTATGGGGCT TCTGTTGCTT ATGAAAATGC CCTGCGGGTG
    TTCAACATCG TCTTCACCTC CCTCTTCTCT CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTCCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ATATCCTCGT GACTGAGTTT GGGAATCCGA ATAACTTCAT CAACCTGAGC
    TTTCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTTC TCCGTCAGGG TTACACCATC
    CGCATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCTTATGT CTGTCTGCTG
    ATCGCCATGC TCTTCTTCAT CTATGCCATC ATTGGGATGC AGGTGTTTGG TAACATTGGC
    ATCGACGTGG AGGACGAGGA CAGTGATGAA GATGAGTTCC AAATCACTGA GCACAATAAC
    TTCCGGACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACCGG GGAAGCTTGG
    CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCGTGTG ATAAGAACTC TGGCATCCTG
    ACTCGAGAGT GTGGCAATGA ATTTGCTTAT TTTTACTTTG TTTCCTTCAT CTTCCTCTGC
    TCGTTTCTGA TGCTGAATCT CTTTGTCGCC GTCATCATGG ACAACTTTGA GTACCTCACC
    CGAGACTCCT CCATCCTGGG CCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG
    TATGACCCCG CAGCTTGGGG CCGCATGCCT TACCTGGACA TGTATCAGAT GCTGAGACAC
    ATGTCTCCGC CCCTGGGTCT GGGGAAGAAG TGTCCGGCCA GAGTGGCTTA CAAGCGGCTT
    CTGCGGATGG ACCTGCCCGT CGCAGATGAC AACACCGTCC ACTTCAATTC CACCCTCATG
    GCTCTGATCC GCACAGCCCT GGACATCAAG ATTGCCAAGG GAGGAGCCGA CAAACAGCAG
    ATGGACGCTG AGCTGCGGAA GGAGATGATG GCGATTTGGC CCAATCTGTC CCAGAAGACG
    CTAGACCTGC TGGTCACACC TCACAAGTCC ACGGACCTCA CCGTGGGGAA GATCTACGCA
    GCCATGATGA TCATGGAGTA CTACCGGCAG AGCAAGGCCA AGAAGCTGCA GGCCATGCGC
    GAGGAGCAGG ACCGGACACC CCTCATGTTC CAGCGCATGG AGCCCCCGTC CCCAACGCAG
    GAAGGGGGAC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGCCCTG
    ATGGCTCACG AAAGCGGCCT CAAGGAGAGC CCGTCCTGGG TGACCCAGCG TGCCCAGGAG
    ATGTTCCAGA AGACGGGCAC ATGGAGTCCG GAACAAGGCC CCCCTACCGA CATGCCCAAC
    AGCCAGCCTA ACTCTCAGTC CGTGGAGATG CGAGAGATGG GCAGAGATGG CTACTCCGAC
    AGCGAGCACT ACCTCCCCAT GGAAGGCCAG GGCCGGGCTG CCTCCATGCC CCGCCTCCCT
    GCAGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC
    ACCAGCCCCA TGAAGCGTTC AGCCTCCGTG CTGGGCCCCA AGGCCCGACG CCTGGACGAT
    TACTCGCTGG AGCGGGTCCC GCCCGAGGAG AACCAGCGGC ACCACCAGCG GCGCCGCGAC
    CGCAGCCACC GCGCCTCTGA GCGCTCCCTG GGCCGCTACA CCGATGTGGA CACAGGCTTG
    GGGACAGACC TGAGCATGAC CACCCAATCC GGGGACCTGC CGTCGAAGGA GCGGGACCAG
    GAGCGGGGCC GGCCCAAGGA TCGGAAGCAT CGACAGCACC ACCACCACCA CCACCACCAC
    CACCATCCCC CGCCCCCCGA CAAGGACCGC TATGCCCAGG AACGGCCGGA CCACGGCCGG
    GCACGGGCTC GGGACCAGCG CTGGTCCCGC TCGCCCAGCG AGGGCCGAGA GCACATGGCG
    CACCGGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21065
    Clone ID Related Accession (Same CDS sequence) NM_001174080.1 , NM_001174080.2
    Accession Version NM_001174080.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008540.6, AF004883.1, FJ040507.1 and AC011446.6. On Jun 2, 2019 this sequence version replaced NM_001174080.1. Transcript Variant: This variant (5) uses two alternate splice sites, compared to variant 2. The resulting isoform (5) lacks an internal 3-aa segment and has a shorter and distinct C-terminus that does not include the polyglutamine tract, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGACGA GCAAGAGGAA GAAGAAGCAG CGAACCAGAA ACTTGCCCTA
    CAGAAAGCCA AGGAGGTGGC AGAAGTGAGT CCTCTGTCCG CGGCCAACAT GTCTATAGCT
    GTGAAAGAGC AACAGAAGAA TCAAAAGCCA GCCAAGTCCG TGTGGGAGCA GCGGACCAGT
    GAGATGCGAA AGCAGAACTT GCTGGCCAGC CGGGAGGCCC TGTATAACGA AATGGACCCG
    GACGAGCGCT GGAAGGCTGC CTACACGCGG CACCTGCGGC CAGACATGAA GACGCACTTG
    GACCGGCCGC TGGTGGTGGA CCCGCAGGAG AACCGCAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACCGTGGA CCAGCGCCTC GGCCAGCAGC GCGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA TCGGGCCCGG GACCCCAGCG GCTCGGCGGG CCTGGACGCA
    CGGAGGCCCT GGGCGGGAAG CCAGGAGGCC GAGCTGAGCC GGGAGGGACC CTACGGCCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCAGC CTGGAGCAAC CCGGGTTCTG GGAGGGCGAG
    GCCGAGCGAG GCAAGGCCGG GGACCCCCAC CGGAGGCACG TGCACCGGCA GGGGGGCAGC
    AGGGAGAGCC GCAGCGGGTC CCCGCGCACG GGCGCGGACG GGGAGCATCG ACGTCATCGC
    GCGCACCGCA GGCCCGGGGA GGAGGGTCCG GAGGACAAGG CGGAGCGGAG GGCGCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGG GCGAGGGCCC CGACGGGGGC
    GAGCGCAGGA GAAGGCACCG GCATGGCGCT CCAGCCACGT ACGAGGGGGA CGCGCGGAGG
    GAGGACAAGG AGCGGAGGCA TCGGAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCTGTG
    TCGGGCCCCA ACCTGTCAAC CACCCGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCA
    CCCCTGGCAG AGGATATTGA CAACATGAAG AACAACAAGC TGGCCACCGC GGAGTCGGCC
    GCTCCCCACG GCAGCCTTGG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGAAAC
    AGCACCGACC CCGGCCCCAT GCTGGCCATC CCTGCCATGG CCACCAACCC CCAGAACGCC
    GCCAGCCGCC GGACGCCCAA CAACCCGGGG AACCCATCCA ATCCCGGCCC CCCCAAGACC
    CCCGAGAATA GCCTTATCGT CACCAACCCC AGCGGCACCC AGACCAATTC AGCTAAGACT
    GCCAGGAAAC CCGACCACAC CACAGTGGAC ATCCCCCCAG CCTGCCCACC CCCCCTCAAC
    CACACCGTCG TACAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAAGAGGAA
    GAGAAGAAGG AGGAGGAGGA AGACGACCGT GGGGAAGACG GCCCTAAGCC AATGCCTCCC
    TATAGCTCCA TGTTCATCCT GTCCACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATC CTCATGGTCA TTGCCATGAG CAGCATCGCC
    CTGGCCGCCG AGGACCCTGT GCAGCCCAAC GCACCTCGGA ACAACGTGCT GCGATACTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT TGACCTGGGG
    CTCGTCCTGC ATCAGGGTGC CTACTTCCGT GACCTCTGGA ATATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTAGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA AGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGCTG
    CCAAAGCTCA AGGCTGTGTT TGACTGTGTG GTGAACTCAC TTAAAAACGT CTTCAACATC
    CTCATCGTCT ACATGCTATT CATGTTCATC TTCGCCGTGG TGGCTGTGCA GCTCTTCAAG
    GGGAAATTCT TCCACTGCAC TGACGAGTCC AAAGAGTTTG AGAAAGATTG TCGAGGCAAA
    TACCTCCTCT ACGAGAAGAA TGAGGTGAAG GCGCGAGACC GGGAGTGGAA GAAGTATGAA
    TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCATTCGGTG GACGCCACCT TTGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATTTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ATATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AATACAGCCT GGAGAAAAAT GAGAGGGCCT GCATTGATTT CGCCATCAGC
    GCCAAGCCGC TGACCCGACA CATGCCGCAG AACAAGCAGA GCTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCTCCGCC TTTCGAGTAC ACGATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTTA TGATGAAGTT CTATGGGGCT TCTGTTGCTT ATGAAAATGC CCTGCGGGTG
    TTCAACATCG TCTTCACCTC CCTCTTCTCT CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTCCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ATATCCTCGT GACTGAGTTT GGGAATCCGA ATAACTTCAT CAACCTGAGC
    TTTCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTTC TCCGTCAGGG TTACACCATC
    CGCATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCTTATGT CTGTCTGCTG
    ATCGCCATGC TCTTCTTCAT CTATGCCATC ATTGGGATGC AGGTGTTTGG TAACATTGGC
    ATCGACGTGG AGGACGAGGA CAGTGATGAA GATGAGTTCC AAATCACTGA GCACAATAAC
    TTCCGGACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACCGG GGAAGCTTGG
    CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCGTGTG ATAAGAACTC TGGCATCCTG
    ACTCGAGAGT GTGGCAATGA ATTTGCTTAT TTTTACTTTG TTTCCTTCAT CTTCCTCTGC
    TCGTTTCTGA TGCTGAATCT CTTTGTCGCC GTCATCATGG ACAACTTTGA GTACCTCACC
    CGAGACTCCT CCATCCTGGG CCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG
    TATGACCCCG CAGCTTGGGG CCGCATGCCT TACCTGGACA TGTATCAGAT GCTGAGACAC
    ATGTCTCCGC CCCTGGGTCT GGGGAAGAAG TGTCCGGCCA GAGTGGCTTA CAAGCGGCTT
    CTGCGGATGG ACCTGCCCGT CGCAGATGAC AACACCGTCC ACTTCAATTC CACCCTCATG
    GCTCTGATCC GCACAGCCCT GGACATCAAG ATTGCCAAGG GAGGAGCCGA CAAACAGCAG
    ATGGACGCTG AGCTGCGGAA GGAGATGATG GCGATTTGGC CCAATCTGTC CCAGAAGACG
    CTAGACCTGC TGGTCACACC TCACAAGTCC ACGGACCTCA CCGTGGGGAA GATCTACGCA
    GCCATGATGA TCATGGAGTA CTACCGGCAG AGCAAGGCCA AGAAGCTGCA GGCCATGCGC
    GAGGAGCAGG ACCGGACACC CCTCATGTTC CAGCGCATGG AGCCCCCGTC CCCAACGCAG
    GAAGGGGGAC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGCCCTG
    ATGGCTCACG AAAGCGGCCT CAAGGAGAGC CCGTCCTGGG TGACCCAGCG TGCCCAGGAG
    ATGTTCCAGA AGACGGGCAC ATGGAGTCCG GAACAAGGCC CCCCTACCGA CATGCCCAAC
    AGCCAGCCTA ACTCTCAGTC CGTGGAGATG CGAGAGATGG GCAGAGATGG CTACTCCGAC
    AGCGAGCACT ACCTCCCCAT GGAAGGCCAG GGCCGGGCTG CCTCCATGCC CCGCCTCCCT
    GCAGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC
    ACCAGCCCCA TGAAGCGTTC AGCCTCCGTG CTGGGCCCCA AGGCCCGACG CCTGGACGAT
    TACTCGCTGG AGCGGGTCCC GCCCGAGGAG AACCAGCGGC ACCACCAGCG GCGCCGCGAC
    CGCAGCCACC GCGCCTCTGA GCGCTCCCTG GGCCGCTACA CCGATGTGGA CACAGGCTTG
    GGGACAGACC TGAGCATGAC CACCCAATCC GGGGACCTGC CGTCGAAGGA GCGGGACCAG
    GAGCGGGGCC GGCCCAAGGA TCGGAAGCAT CGACAGCACC ACCACCACCA CCACCACCAC
    CACCATCCCC CGCCCCCCGA CAAGGACCGC TATGCCCAGG AACGGCCGGA CCACGGCCGG
    GCACGGGCTC GGGACCAGCG CTGGTCCCGC TCGCCCAGCG AGGGCCGAGA GCACATGGCG
    CACCGGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001167551.1
    CDS256..7047
    Translation

    Target ORF information:

    RefSeq Version NM_001174080.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001174080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGACGA GCAAGAGGAA GAAGAAGCAG CGAACCAGAA ACTTGCCCTA
    CAGAAAGCCA AGGAGGTGGC AGAAGTGAGT CCTCTGTCCG CGGCCAACAT GTCTATAGCT
    GTGAAAGAGC AACAGAAGAA TCAAAAGCCA GCCAAGTCCG TGTGGGAGCA GCGGACCAGT
    GAGATGCGAA AGCAGAACTT GCTGGCCAGC CGGGAGGCCC TGTATAACGA AATGGACCCG
    GACGAGCGCT GGAAGGCTGC CTACACGCGG CACCTGCGGC CAGACATGAA GACGCACTTG
    GACCGGCCGC TGGTGGTGGA CCCGCAGGAG AACCGCAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACCGTGGA CCAGCGCCTC GGCCAGCAGC GCGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA TCGGGCCCGG GACCCCAGCG GCTCGGCGGG CCTGGACGCA
    CGGAGGCCCT GGGCGGGAAG CCAGGAGGCC GAGCTGAGCC GGGAGGGACC CTACGGCCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCAGC CTGGAGCAAC CCGGGTTCTG GGAGGGCGAG
    GCCGAGCGAG GCAAGGCCGG GGACCCCCAC CGGAGGCACG TGCACCGGCA GGGGGGCAGC
    AGGGAGAGCC GCAGCGGGTC CCCGCGCACG GGCGCGGACG GGGAGCATCG ACGTCATCGC
    GCGCACCGCA GGCCCGGGGA GGAGGGTCCG GAGGACAAGG CGGAGCGGAG GGCGCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGG GCGAGGGCCC CGACGGGGGC
    GAGCGCAGGA GAAGGCACCG GCATGGCGCT CCAGCCACGT ACGAGGGGGA CGCGCGGAGG
    GAGGACAAGG AGCGGAGGCA TCGGAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCTGTG
    TCGGGCCCCA ACCTGTCAAC CACCCGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCA
    CCCCTGGCAG AGGATATTGA CAACATGAAG AACAACAAGC TGGCCACCGC GGAGTCGGCC
    GCTCCCCACG GCAGCCTTGG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGAAAC
    AGCACCGACC CCGGCCCCAT GCTGGCCATC CCTGCCATGG CCACCAACCC CCAGAACGCC
    GCCAGCCGCC GGACGCCCAA CAACCCGGGG AACCCATCCA ATCCCGGCCC CCCCAAGACC
    CCCGAGAATA GCCTTATCGT CACCAACCCC AGCGGCACCC AGACCAATTC AGCTAAGACT
    GCCAGGAAAC CCGACCACAC CACAGTGGAC ATCCCCCCAG CCTGCCCACC CCCCCTCAAC
    CACACCGTCG TACAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAAGAGGAA
    GAGAAGAAGG AGGAGGAGGA AGACGACCGT GGGGAAGACG GCCCTAAGCC AATGCCTCCC
    TATAGCTCCA TGTTCATCCT GTCCACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATC CTCATGGTCA TTGCCATGAG CAGCATCGCC
    CTGGCCGCCG AGGACCCTGT GCAGCCCAAC GCACCTCGGA ACAACGTGCT GCGATACTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT TGACCTGGGG
    CTCGTCCTGC ATCAGGGTGC CTACTTCCGT GACCTCTGGA ATATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTAGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA AGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGCTG
    CCAAAGCTCA AGGCTGTGTT TGACTGTGTG GTGAACTCAC TTAAAAACGT CTTCAACATC
    CTCATCGTCT ACATGCTATT CATGTTCATC TTCGCCGTGG TGGCTGTGCA GCTCTTCAAG
    GGGAAATTCT TCCACTGCAC TGACGAGTCC AAAGAGTTTG AGAAAGATTG TCGAGGCAAA
    TACCTCCTCT ACGAGAAGAA TGAGGTGAAG GCGCGAGACC GGGAGTGGAA GAAGTATGAA
    TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCATTCGGTG GACGCCACCT TTGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATTTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ATATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AATACAGCCT GGAGAAAAAT GAGAGGGCCT GCATTGATTT CGCCATCAGC
    GCCAAGCCGC TGACCCGACA CATGCCGCAG AACAAGCAGA GCTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCTCCGCC TTTCGAGTAC ACGATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTTA TGATGAAGTT CTATGGGGCT TCTGTTGCTT ATGAAAATGC CCTGCGGGTG
    TTCAACATCG TCTTCACCTC CCTCTTCTCT CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTCCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ATATCCTCGT GACTGAGTTT GGGAATCCGA ATAACTTCAT CAACCTGAGC
    TTTCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTTC TCCGTCAGGG TTACACCATC
    CGCATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCTTATGT CTGTCTGCTG
    ATCGCCATGC TCTTCTTCAT CTATGCCATC ATTGGGATGC AGGTGTTTGG TAACATTGGC
    ATCGACGTGG AGGACGAGGA CAGTGATGAA GATGAGTTCC AAATCACTGA GCACAATAAC
    TTCCGGACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACCGG GGAAGCTTGG
    CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCGTGTG ATAAGAACTC TGGCATCCTG
    ACTCGAGAGT GTGGCAATGA ATTTGCTTAT TTTTACTTTG TTTCCTTCAT CTTCCTCTGC
    TCGTTTCTGA TGCTGAATCT CTTTGTCGCC GTCATCATGG ACAACTTTGA GTACCTCACC
    CGAGACTCCT CCATCCTGGG CCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG
    TATGACCCCG CAGCTTGGGG CCGCATGCCT TACCTGGACA TGTATCAGAT GCTGAGACAC
    ATGTCTCCGC CCCTGGGTCT GGGGAAGAAG TGTCCGGCCA GAGTGGCTTA CAAGCGGCTT
    CTGCGGATGG ACCTGCCCGT CGCAGATGAC AACACCGTCC ACTTCAATTC CACCCTCATG
    GCTCTGATCC GCACAGCCCT GGACATCAAG ATTGCCAAGG GAGGAGCCGA CAAACAGCAG
    ATGGACGCTG AGCTGCGGAA GGAGATGATG GCGATTTGGC CCAATCTGTC CCAGAAGACG
    CTAGACCTGC TGGTCACACC TCACAAGTCC ACGGACCTCA CCGTGGGGAA GATCTACGCA
    GCCATGATGA TCATGGAGTA CTACCGGCAG AGCAAGGCCA AGAAGCTGCA GGCCATGCGC
    GAGGAGCAGG ACCGGACACC CCTCATGTTC CAGCGCATGG AGCCCCCGTC CCCAACGCAG
    GAAGGGGGAC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGCCCTG
    ATGGCTCACG AAAGCGGCCT CAAGGAGAGC CCGTCCTGGG TGACCCAGCG TGCCCAGGAG
    ATGTTCCAGA AGACGGGCAC ATGGAGTCCG GAACAAGGCC CCCCTACCGA CATGCCCAAC
    AGCCAGCCTA ACTCTCAGTC CGTGGAGATG CGAGAGATGG GCAGAGATGG CTACTCCGAC
    AGCGAGCACT ACCTCCCCAT GGAAGGCCAG GGCCGGGCTG CCTCCATGCC CCGCCTCCCT
    GCAGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC
    ACCAGCCCCA TGAAGCGTTC AGCCTCCGTG CTGGGCCCCA AGGCCCGACG CCTGGACGAT
    TACTCGCTGG AGCGGGTCCC GCCCGAGGAG AACCAGCGGC ACCACCAGCG GCGCCGCGAC
    CGCAGCCACC GCGCCTCTGA GCGCTCCCTG GGCCGCTACA CCGATGTGGA CACAGGCTTG
    GGGACAGACC TGAGCATGAC CACCCAATCC GGGGACCTGC CGTCGAAGGA GCGGGACCAG
    GAGCGGGGCC GGCCCAAGGA TCGGAAGCAT CGACAGCACC ACCACCACCA CCACCACCAC
    CACCATCCCC CGCCCCCCGA CAAGGACCGC TATGCCCAGG AACGGCCGGA CCACGGCCGG
    GCACGGGCTC GGGACCAGCG CTGGTCCCGC TCGCCCAGCG AGGGCCGAGA GCACATGGCG
    CACCGGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25901
    Clone ID Related Accession (Same CDS sequence) NM_000068.4 , NM_000068.3
    Accession Version NM_000068.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-30
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008540.6, AF004883.1, BQ340521.1, AB035726.1 and AC011446.6. On Jun 2, 2019 this sequence version replaced NM_000068.3. Transcript Variant: This variant (1) uses an alternate splice site in the 3' coding region that results in a frameshift, compared to variant 2. The resulting isoform (1) has a shorter and distinct C-terminus that does not include the polyglutamine tract, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF004883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000059.3
    CDS256..7056
    Translation

    Target ORF information:

    RefSeq Version NM_000068.4
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000068.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25901
    Clone ID Related Accession (Same CDS sequence) NM_000068.4 , NM_000068.3
    Accession Version NM_000068.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF004883.1, BQ340521.1, AB035726.1 and AC011446.6. On Jun 1, 2007 this sequence version replaced NM_000068.2. Transcript Variant: This variant (1) uses an alternate splice site in the 3' coding region that results in a frameshift, compared to variant 2. The resulting isoform (1) has a shorter and distinct C-terminus that does not include the polyglutamine tract, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF004883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000059.3
    CDS237..7037
    Translation

    Target ORF information:

    RefSeq Version NM_000068.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000068.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGTGGA GGCGGACGAG CAAGAGGAAG AAGAAGCAGC GAACCAGAAA
    CTTGCCCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CTCTGTCCGC GGCCAACATG
    TCTATAGCTG TGAAAGAGCA ACAGAAGAAT CAAAAGCCAG CCAAGTCCGT GTGGGAGCAG
    CGGACCAGTG AGATGCGAAA GCAGAACTTG CTGGCCAGCC GGGAGGCCCT GTATAACGAA
    ATGGACCCGG ACGAGCGCTG GAAGGCTGCC TACACGCGGC ACCTGCGGCC AGACATGAAG
    ACGCACTTGG ACCGGCCGCT GGTGGTGGAC CCGCAGGAGA ACCGCAACAA CAACACCAAC
    AAGAGCCGGG CGGCCGAGCC CACCGTGGAC CAGCGCCTCG GCCAGCAGCG CGCCGAGGAC
    TTCCTCAGGA AACAGGCCCG CTACCACGAT CGGGCCCGGG ACCCCAGCGG CTCGGCGGGC
    CTGGACGCAC GGAGGCCCTG GGCGGGAAGC CAGGAGGCCG AGCTGAGCCG GGAGGGACCC
    TACGGCCGCG AGTCGGACCA CCACGCCCGG GAGGGCAGCC TGGAGCAACC CGGGTTCTGG
    GAGGGCGAGG CCGAGCGAGG CAAGGCCGGG GACCCCCACC GGAGGCACGT GCACCGGCAG
    GGGGGCAGCA GGGAGAGCCG CAGCGGGTCC CCGCGCACGG GCGCGGACGG GGAGCATCGA
    CGTCATCGCG CGCACCGCAG GCCCGGGGAG GAGGGTCCGG AGGACAAGGC GGAGCGGAGG
    GCGCGGCACC GCGAGGGCAG CCGGCCGGCC CGGGGCGGCG AGGGCGAGGG CGAGGGCCCC
    GACGGGGGCG AGCGCAGGAG AAGGCACCGG CATGGCGCTC CAGCCACGTA CGAGGGGGAC
    GCGCGGAGGG AGGACAAGGA GCGGAGGCAT CGGAGGAGGA AAGAGAACCA GGGCTCCGGG
    GTCCCTGTGT CGGGCCCCAA CCTGTCAACC ACCCGGCCAA TCCAGCAGGA CCTGGGCCGC
    CAAGACCCAC CCCTGGCAGA GGATATTGAC AACATGAAGA ACAACAAGCT GGCCACCGCG
    GAGTCGGCCG CTCCCCACGG CAGCCTTGGC CACGCCGGCC TGCCCCAGAG CCCAGCCAAG
    ATGGGAAACA GCACCGACCC CGGCCCCATG CTGGCCATCC CTGCCATGGC CACCAACCCC
    CAGAACGCCG CCAGCCGCCG GACGCCCAAC AACCCGGGGA ACCCATCCAA TCCCGGCCCC
    CCCAAGACCC CCGAGAATAG CCTTATCGTC ACCAACCCCA GCGGCACCCA GACCAATTCA
    GCTAAGACTG CCAGGAAACC CGACCACACC ACAGTGGACA TCCCCCCAGC CTGCCCACCC
    CCCCTCAACC ACACCGTCGT ACAAGTGAAC AAAAACGCCA ACCCAGACCC ACTGCCAAAA
    AAAGAGGAAG AGAAGAAGGA GGAGGAGGAA GACGACCGTG GGGAAGACGG CCCTAAGCCA
    ATGCCTCCCT ATAGCTCCAT GTTCATCCTG TCCACGACCA ACCCCCTTCG CCGCCTGTGC
    CATTACATCC TGAACCTGCG CTACTTTGAG ATGTGCATCC TCATGGTCAT TGCCATGAGC
    AGCATCGCCC TGGCCGCCGA GGACCCTGTG CAGCCCAACG CACCTCGGAA CAACGTGCTG
    CGATACTTTG ACTACGTTTT TACAGGCGTC TTTACCTTTG AGATGGTGAT CAAGATGATT
    GACCTGGGGC TCGTCCTGCA TCAGGGTGCC TACTTCCGTG ACCTCTGGAA TATTCTCGAC
    TTCATAGTGG TCAGTGGGGC CCTGGTAGCC TTTGCCTTCA CTGGCAATAG CAAAGGAAAA
    GACATCAACA CGATTAAATC CCTCCGAGTC CTCCGGGTGC TACGACCTCT TAAAACCATC
    AAGCGGCTGC CAAAGCTCAA GGCTGTGTTT GACTGTGTGG TGAACTCACT TAAAAACGTC
    TTCAACATCC TCATCGTCTA CATGCTATTC ATGTTCATCT TCGCCGTGGT GGCTGTGCAG
    CTCTTCAAGG GGAAATTCTT CCACTGCACT GACGAGTCCA AAGAGTTTGA GAAAGATTGT
    CGAGGCAAAT ACCTCCTCTA CGAGAAGAAT GAGGTGAAGG CGCGAGACCG GGAGTGGAAG
    AAGTATGAAT TCCATTACGA CAATGTGCTG TGGGCTCTGC TGACCCTCTT CACCGTGTCC
    ACGGGAGAAG GCTGGCCACA GGTCCTCAAG CATTCGGTGG ACGCCACCTT TGAGAACCAG
    GGCCCCAGCC CCGGGTACCG CATGGAGATG TCCATTTTCT ACGTCGTCTA CTTTGTGGTG
    TTCCCCTTCT TCTTTGTCAA TATCTTTGTG GCCTTGATCA TCATCACCTT CCAGGAGCAA
    GGGGACAAGA TGATGGAGGA ATACAGCCTG GAGAAAAATG AGAGGGCCTG CATTGATTTC
    GCCATCAGCG CCAAGCCGCT GACCCGACAC ATGCCGCAGA ACAAGCAGAG CTTCCAGTAC
    CGCATGTGGC AGTTCGTGGT GTCTCCGCCT TTCGAGTACA CGATCATGGC CATGATCGCC
    CTCAACACCA TCGTGCTTAT GATGAAGTTC TATGGGGCTT CTGTTGCTTA TGAAAATGCC
    CTGCGGGTGT TCAACATCGT CTTCACCTCC CTCTTCTCTC TGGAATGTGT GCTGAAAGTC
    ATGGCTTTTG GGATTCTGAA TTATTTCCGC GATGCCTGGA ACATCTTCGA CTTTGTGACT
    GTTCTGGGCA GCATCACCGA TATCCTCGTG ACTGAGTTTG GGAATCCGAA TAACTTCATC
    AACCTGAGCT TTCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTTCT CCGTCAGGGT
    TACACCATCC GCATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCTTATGTC
    TGTCTGCTGA TCGCCATGCT CTTCTTCATC TATGCCATCA TTGGGATGCA GGTGTTTGGT
    AACATTGGCA TCGACGTGGA GGACGAGGAC AGTGATGAAG ATGAGTTCCA AATCACTGAG
    CACAATAACT TCCGGACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACCGGG
    GAAGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCGTGTGA TAAGAACTCT
    GGCATCCTGA CTCGAGAGTG TGGCAATGAA TTTGCTTATT TTTACTTTGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTC TTTGTCGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGACTCCTC CATCCTGGGC CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCCGC AGCTTGGGGC CGCATGCCTT ACCTGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGCGGCTTC TGCGGATGGA CCTGCCCGTC GCAGATGACA ACACCGTCCA CTTCAATTCC
    ACCCTCATGG CTCTGATCCG CACAGCCCTG GACATCAAGA TTGCCAAGGG AGGAGCCGAC
    AAACAGCAGA TGGACGCTGA GCTGCGGAAG GAGATGATGG CGATTTGGCC CAATCTGTCC
    CAGAAGACGC TAGACCTGCT GGTCACACCT CACAAGTCCA CGGACCTCAC CGTGGGGAAG
    ATCTACGCAG CCATGATGAT CATGGAGTAC TACCGGCAGA GCAAGGCCAA GAAGCTGCAG
    GCCATGCGCG AGGAGCAGGA CCGGACACCC CTCATGTTCC AGCGCATGGA GCCCCCGTCC
    CCAACGCAGG AAGGGGGACC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGCCCTGA TGGCTCACGA AAGCGGCCTC AAGGAGAGCC CGTCCTGGGT GACCCAGCGT
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGTCCGG AACAAGGCCC CCCTACCGAC
    ATGCCCAACA GCCAGCCTAA CTCTCAGTCC GTGGAGATGC GAGAGATGGG CAGAGATGGC
    TACTCCGACA GCGAGCACTA CCTCCCCATG GAAGGCCAGG GCCGGGCTGC CTCCATGCCC
    CGCCTCCCTG CAGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGTTCA GCCTCCGTGC TGGGCCCCAA GGCCCGACGC
    CTGGACGATT ACTCGCTGGA GCGGGTCCCG CCCGAGGAGA ACCAGCGGCA CCACCAGCGG
    CGCCGCGACC GCAGCCACCG CGCCTCTGAG CGCTCCCTGG GCCGCTACAC CGATGTGGAC
    ACAGGCTTGG GGACAGACCT GAGCATGACC ACCCAATCCG GGGACCTGCC GTCGAAGGAG
    CGGGACCAGG AGCGGGGCCG GCCCAAGGAT CGGAAGCATC GACAGCACCA CCACCACCAC
    CACCACCACC ACCATCCCCC GCCCCCCGAC AAGGACCGCT ATGCCCAGGA ACGGCCGGAC
    CACGGCCGGG CACGGGCTCG GGACCAGCGC TGGTCCCGCT CGCCCAGCGA GGGCCGAGAG
    CACATGGCGC ACCGGCAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26122
    Clone ID Related Accession (Same CDS sequence) NM_001127222.1 , NM_001127222.2
    Accession Version NM_001127222.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF004883.1, AB035727.2, AC022436.6, AC124224.1, AC026805.5 and AC011446.6. Transcript Variant: This variant (4) uses two alternate in-frame splice sites and lacks an alternate in-frame exon, compared to variant 2. The resulting isoform (4) differs at three internal regions, compared to isoform 2, and includes the polyglutamine tract near the C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035727.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGCTCTG
    CGGAGAACCA CCATAAAGAA AAGCAAGACA GATTTGCTCA ACCCCGAAGA GGCTGAGGAT
    CAGCTGGCTG ATATAGCCTC TGTGGGTTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCGACCTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTACTCA GTTTGGTAGC TCTCAACACG
    CTGTGTGTTG CTATTGTTCA CTACAACCAG CCCGAGTGGC TCTCCGACTT CCTTTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGCTTTG ACTGTGGGGT TATCATTGGG
    AGCATCTTCG AGGTCATCTG GGCTGTCATA AAACCTGGCA CATCCTTTGG AATCAGCGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGA
    AACCTGGTCG TCTCTCTCCT CAACTCCATG AAGTCCATCA TCAGCCTGTT GTTTCTCCTT
    TTCCTGTTCA TTGTCGTCTT CGCCCTTTTG GGAATGCAAC TCTTCGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC CACCAACTTC GATACTTTTC CAGCAGCAAT AATGACGGTG
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ACGACGGGAT CAAGTCTCAG
    GGGGGCGTGC AGGGCGGCAT GGTGTTCTCC ATCTATTTCA TTGTACTGAC GCTCTTTGGG
    AACTACACCC TCCTGAATGT GTTCTTGGCC ATCGCTGTGG ACAATCTGGC CAACGCCCAG
    GAGCTCACCA AGGACGAGCA AGAGGAAGAA GAAGCAGCGA ACCAGAAACT TGCCCTACAG
    AAAGCCAAGG AGGTGGCAGA AGTGAGTCCT CTGTCCGCGG CCAACATGTC TATAGCTGTG
    AAAGAGCAAC AGAAGAATCA AAAGCCAGCC AAGTCCGTGT GGGAGCAGCG GACCAGTGAG
    ATGCGAAAGC AGAACTTGCT GGCCAGCCGG GAGGCCCTGT ATAACGAAAT GGACCCGGAC
    GAGCGCTGGA AGGCTGCCTA CACGCGGCAC CTGCGGCCAG ACATGAAGAC GCACTTGGAC
    CGGCCGCTGG TGGTGGACCC GCAGGAGAAC CGCAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CCGTGGACCA GCGCCTCGGC CAGCAGCGCG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGATCG GGCCCGGGAC CCCAGCGGCT CGGCGGGCCT GGACGCACGG
    AGGCCCTGGG CGGGAAGCCA GGAGGCCGAG CTGAGCCGGG AGGGACCCTA CGGCCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCAGCCTG GAGCAACCCG GGTTCTGGGA GGGCGAGGCC
    GAGCGAGGCA AGGCCGGGGA CCCCCACCGG AGGCACGTGC ACCGGCAGGG GGGCAGCAGG
    GAGAGCCGCA GCGGGTCCCC GCGCACGGGC GCGGACGGGG AGCATCGACG TCATCGCGCG
    CACCGCAGGC CCGGGGAGGA GGGTCCGGAG GACAAGGCGG AGCGGAGGGC GCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGGGCG AGGGCCCCGA CGGGGGCGAG
    CGCAGGAGAA GGCACCGGCA TGGCGCTCCA GCCACGTACG AGGGGGACGC GCGGAGGGAG
    GACAAGGAGC GGAGGCATCG GAGGAGGAAA GAGAACCAGG GCTCCGGGGT CCCTGTGTCG
    GGCCCCAACC TGTCAACCAC CCGGCCAATC CAGCAGGACC TGGGCCGCCA AGACCCACCC
    CTGGCAGAGG ATATTGACAA CATGAAGAAC AACAAGCTGG CCACCGCGGA GTCGGCCGCT
    CCCCACGGCA GCCTTGGCCA CGCCGGCCTG CCCCAGAGCC CAGCCAAGAT GGGAAACAGC
    ACCGACCCCG GCCCCATGCT GGCCATCCCT GCCATGGCCA CCAACCCCCA GAACGCCGCC
    AGCCGCCGGA CGCCCAACAA CCCGGGGAAC CCATCCAATC CCGGCCCCCC CAAGACCCCC
    GAGAATAGCC TTATCGTCAC CAACCCCAGC GGCACCCAGA CCAATTCAGC TAAGACTGCC
    AGGAAACCCG ACCACACCAC AGTGGACATC CCCCCAGCCT GCCCACCCCC CCTCAACCAC
    ACCGTCGTAC AAGTGAACAA AAACGCCAAC CCAGACCCAC TGCCAAAAAA AGAGGAAGAG
    AAGAAGGAGG AGGAGGAAGA CGACCGTGGG GAAGACGGCC CTAAGCCAAT GCCTCCCTAT
    AGCTCCATGT TCATCCTGTC CACGACCAAC CCCCTTCGCC GCCTGTGCCA TTACATCCTG
    AACCTGCGCT ACTTTGAGAT GTGCATCCTC ATGGTCATTG CCATGAGCAG CATCGCCCTG
    GCCGCCGAGG ACCCTGTGCA GCCCAACGCA CCTCGGAACA ACGTGCTGCG ATACTTTGAC
    TACGTTTTTA CAGGCGTCTT TACCTTTGAG ATGGTGATCA AGATGATTGA CCTGGGGCTC
    GTCCTGCATC AGGGTGCCTA CTTCCGTGAC CTCTGGAATA TTCTCGACTT CATAGTGGTC
    AGTGGGGCCC TGGTAGCCTT TGCCTTCACT GGCAATAGCA AAGGAAAAGA CATCAACACG
    ATTAAATCCC TCCGAGTCCT CCGGGTGCTA CGACCTCTTA AAACCATCAA GCGGCTGCCA
    AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG AACTCACTTA AAAACGTCTT CAACATCCTC
    ATCGTCTACA TGCTATTCAT GTTCATCTTC GCCGTGGTGG CTGTGCAGCT CTTCAAGGGG
    AAATTCTTCC ACTGCACTGA CGAGTCCAAA GAGTTTGAGA AAGATTGTCG AGGCAAATAC
    CTCCTCTACG AGAAGAATGA GGTGAAGGCG CGAGACCGGG AGTGGAAGAA GTATGAATTC
    CATTACGACA ATGTGCTGTG GGCTCTGCTG ACCCTCTTCA CCGTGTCCAC GGGAGAAGGC
    TGGCCACAGG TCCTCAAGCA TTCGGTGGAC GCCACCTTTG AGAACCAGGG CCCCAGCCCC
    GGGTACCGCA TGGAGATGTC CATTTTCTAC GTCGTCTACT TTGTGGTGTT CCCCTTCTTC
    TTTGTCAATA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAAGG GGACAAGATG
    ATGGAGGAAT ACAGCCTGGA GAAAAATGAG AGGGCCTGCA TTGATTTCGC CATCAGCGCC
    AAGCCGCTGA CCCGACACAT GCCGCAGAAC AAGCAGAGCT TCCAGTACCG CATGTGGCAG
    TTCGTGGTGT CTCCGCCTTT CGAGTACACG ATCATGGCCA TGATCGCCCT CAACACCATC
    GTGCTTATGA TGAAGTTCTA TGGGGCTTCT GTTGCTTATG AAAATGCCCT GCGGGTGTTC
    AACATCGTCT TCACCTCCCT CTTCTCTCTG GAATGTGTGC TGAAAGTCAT GGCTTTTGGG
    ATTCTGAATT ATTTCCGCGA TGCCTGGAAC ATCTTCGACT TTGTGACTGT TCTGGGCAGC
    ATCACCGATA TCCTCGTGAC TGAGTTTGGG AATAACTTCA TCAACCTGAG CTTTCTCCGC
    CTCTTCCGAG CTGCCCGGCT CATCAAACTT CTCCGTCAGG GTTACACCAT CCGCATTCTT
    CTCTGGACCT TTGTGCAGTC CTTCAAGGCC CTGCCTTATG TCTGTCTGCT GATCGCCATG
    CTCTTCTTCA TCTATGCCAT CATTGGGATG CAGGTGTTTG GTAACATTGG CATCGACGTG
    GAGGACGAGG ACAGTGATGA AGATGAGTTC CAAATCACTG AGCACAATAA CTTCCGGACC
    TTCTTCCAGG CCCTCATGCT TCTCTTCCGG AGTGCCACCG GGGAAGCTTG GCACAACATC
    ATGCTTTCCT GCCTCAGCGG GAAACCGTGT GATAAGAACT CTGGCATCCT GACTCGAGAG
    TGTGGCAATG AATTTGCTTA TTTTTACTTT GTTTCCTTCA TCTTCCTCTG CTCGTTTCTG
    ATGCTGAATC TCTTTGTCGC CGTCATCATG GACAACTTTG AGTACCTCAC CCGAGACTCC
    TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCCGA GTATGACCCC
    GCAGCTTGGG GCCGCATGCC TTACCTGGAC ATGTATCAGA TGCTGAGACA CATGTCTCCG
    CCCCTGGGTC TGGGGAAGAA GTGTCCGGCC AGAGTGGCTT ACAAGCGGCT TCTGCGGATG
    GACCTGCCCG TCGCAGATGA CAACACCGTC CACTTCAATT CCACCCTCAT GGCTCTGATC
    CGCACAGCCC TGGACATCAA GATTGCCAAG GGAGGAGCCG ACAAACAGCA GATGGACGCT
    GAGCTGCGGA AGGAGATGAT GGCGATTTGG CCCAATCTGT CCCAGAAGAC GCTAGACCTG
    CTGGTCACAC CTCACAAGTC CACGGACCTC ACCGTGGGGA AGATCTACGC AGCCATGATG
    ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG CGAGGAGCAG
    GACCGGACAC CCCTCATGTT CCAGCGCATG GAGCCCCCGT CCCCAACGCA GGAAGGGGGA
    CCTGGCCAGA ACGCCCTCCC CTCCACCCAG CTGGACCCAG GAGGAGCCCT GATGGCTCAC
    GAAAGCGGCC TCAAGGAGAG CCCGTCCTGG GTGACCCAGC GTGCCCAGGA GATGTTCCAG
    AAGACGGGCA CATGGAGTCC GGAACAAGGC CCCCCTACCG ACATGCCCAA CAGCCAGCCT
    AACTCTCAGT CCGTGGAGAT GCGAGAGATG GGCAGAGATG GCTACTCCGA CAGCGAGCAC
    TACCTCCCCA TGGAAGGCCA GGGCCGGGCT GCCTCCATGC CCCGCCTCCC TGCAGAGAAC
    CAGAGGAGAA GGGGCCGGCC ACGTGGGAAT AACCTCAGTA CCATCTCAGA CACCAGCCCC
    ATGAAGCGTT CAGCCTCCGT GCTGGGCCCC AAGGCCCGAC GCCTGGACGA TTACTCGCTG
    GAGCGGGTCC CGCCCGAGGA GAACCAGCGG CACCACCAGC GGCGCCGCGA CCGCAGCCAC
    CGCGCCTCTG AGCGCTCCCT GGGCCGCTAC ACCGATGTGG ACACAGGCTT GGGGACAGAC
    CTGAGCATGA CCACCCAATC CGGGGACCTG CCGTCGAAGG AGCGGGACCA GGAGCGGGGC
    CGGCCCAAGG ATCGGAAGCA TCGACAGCAC CACCACCACC ACCACCACCA CCACCATCCC
    CCGCCCCCCG ACAAGGACCG CTATGCCCAG GAACGGCCGG ACCACGGCCG GGCACGGGCT
    CGGGACCAGC GCTGGTCCCG CTCGCCCAGC GAGGGCCGAG AGCACATGGC GCACCGGCAG
    GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG
    CGGGGCCGCC GCCAGCTCCC CCAGACCCCC TCCACCCCCC GGCCACACGT GTCCTATTCC
    CCTGTGATCC GTAAGGCCGG CGGCTCGGGG CCCCCGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGCGGT GGCCAGGCCG GGCCGGGCGG CCACCAGCGG CCCTCGGAGG
    TACCCAGGCC CCACGGCCGA GCCTCTGGCC GGAGATCGGC CGCCCACGGG GGGCCACAGC
    AGCGGCCGCT CGCCCAGGAT GGAGAGGCGG GTCCCAGGCC CGGCCCGGAG CGAGTCCCCC
    AGGGCCTGTC GACACGGCGG GGCCCGGTGG CCGGCATCTG GCCCGCACGT GTCCGAGGGG
    CCCCCGGGTC CCCGGCACCA TGGCTACTAC CGGGGCTCCG ACTACGACGA GGCCGATGGC
    CCGGGCAGCG GGGGCGGCGA GGAGGCCATG GCCGGGGCCT ACGACGCGCC ACCCCCCGTA
    CGACACGCGT CCTCGGGCGC CACCGGGCGC TCGCCCAGGA CTCCCCGGGC CTCGGGCCCG
    GCCTGCGCCT CGCCTTCTCG GCACGGCCGG CGACTCCCCA ACGGCTACTA CCCGGCGCAC
    GGACTGGCCA GGCCCCGCGG GCCGGGCTCC AGGAAGGGCC TGCACGAACC CTACAGCGAG
    AGTGACGATG ATTGGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120694.1
    CDS237..7757
    Translation

    Target ORF information:

    RefSeq Version NM_001127222.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGCTCTG
    CGGAGAACCA CCATAAAGAA AAGCAAGACA GATTTGCTCA ACCCCGAAGA GGCTGAGGAT
    CAGCTGGCTG ATATAGCCTC TGTGGGTTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCGACCTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTACTCA GTTTGGTAGC TCTCAACACG
    CTGTGTGTTG CTATTGTTCA CTACAACCAG CCCGAGTGGC TCTCCGACTT CCTTTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGCTTTG ACTGTGGGGT TATCATTGGG
    AGCATCTTCG AGGTCATCTG GGCTGTCATA AAACCTGGCA CATCCTTTGG AATCAGCGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGA
    AACCTGGTCG TCTCTCTCCT CAACTCCATG AAGTCCATCA TCAGCCTGTT GTTTCTCCTT
    TTCCTGTTCA TTGTCGTCTT CGCCCTTTTG GGAATGCAAC TCTTCGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC CACCAACTTC GATACTTTTC CAGCAGCAAT AATGACGGTG
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ACGACGGGAT CAAGTCTCAG
    GGGGGCGTGC AGGGCGGCAT GGTGTTCTCC ATCTATTTCA TTGTACTGAC GCTCTTTGGG
    AACTACACCC TCCTGAATGT GTTCTTGGCC ATCGCTGTGG ACAATCTGGC CAACGCCCAG
    GAGCTCACCA AGGACGAGCA AGAGGAAGAA GAAGCAGCGA ACCAGAAACT TGCCCTACAG
    AAAGCCAAGG AGGTGGCAGA AGTGAGTCCT CTGTCCGCGG CCAACATGTC TATAGCTGTG
    AAAGAGCAAC AGAAGAATCA AAAGCCAGCC AAGTCCGTGT GGGAGCAGCG GACCAGTGAG
    ATGCGAAAGC AGAACTTGCT GGCCAGCCGG GAGGCCCTGT ATAACGAAAT GGACCCGGAC
    GAGCGCTGGA AGGCTGCCTA CACGCGGCAC CTGCGGCCAG ACATGAAGAC GCACTTGGAC
    CGGCCGCTGG TGGTGGACCC GCAGGAGAAC CGCAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CCGTGGACCA GCGCCTCGGC CAGCAGCGCG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGATCG GGCCCGGGAC CCCAGCGGCT CGGCGGGCCT GGACGCACGG
    AGGCCCTGGG CGGGAAGCCA GGAGGCCGAG CTGAGCCGGG AGGGACCCTA CGGCCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCAGCCTG GAGCAACCCG GGTTCTGGGA GGGCGAGGCC
    GAGCGAGGCA AGGCCGGGGA CCCCCACCGG AGGCACGTGC ACCGGCAGGG GGGCAGCAGG
    GAGAGCCGCA GCGGGTCCCC GCGCACGGGC GCGGACGGGG AGCATCGACG TCATCGCGCG
    CACCGCAGGC CCGGGGAGGA GGGTCCGGAG GACAAGGCGG AGCGGAGGGC GCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGGGCG AGGGCCCCGA CGGGGGCGAG
    CGCAGGAGAA GGCACCGGCA TGGCGCTCCA GCCACGTACG AGGGGGACGC GCGGAGGGAG
    GACAAGGAGC GGAGGCATCG GAGGAGGAAA GAGAACCAGG GCTCCGGGGT CCCTGTGTCG
    GGCCCCAACC TGTCAACCAC CCGGCCAATC CAGCAGGACC TGGGCCGCCA AGACCCACCC
    CTGGCAGAGG ATATTGACAA CATGAAGAAC AACAAGCTGG CCACCGCGGA GTCGGCCGCT
    CCCCACGGCA GCCTTGGCCA CGCCGGCCTG CCCCAGAGCC CAGCCAAGAT GGGAAACAGC
    ACCGACCCCG GCCCCATGCT GGCCATCCCT GCCATGGCCA CCAACCCCCA GAACGCCGCC
    AGCCGCCGGA CGCCCAACAA CCCGGGGAAC CCATCCAATC CCGGCCCCCC CAAGACCCCC
    GAGAATAGCC TTATCGTCAC CAACCCCAGC GGCACCCAGA CCAATTCAGC TAAGACTGCC
    AGGAAACCCG ACCACACCAC AGTGGACATC CCCCCAGCCT GCCCACCCCC CCTCAACCAC
    ACCGTCGTAC AAGTGAACAA AAACGCCAAC CCAGACCCAC TGCCAAAAAA AGAGGAAGAG
    AAGAAGGAGG AGGAGGAAGA CGACCGTGGG GAAGACGGCC CTAAGCCAAT GCCTCCCTAT
    AGCTCCATGT TCATCCTGTC CACGACCAAC CCCCTTCGCC GCCTGTGCCA TTACATCCTG
    AACCTGCGCT ACTTTGAGAT GTGCATCCTC ATGGTCATTG CCATGAGCAG CATCGCCCTG
    GCCGCCGAGG ACCCTGTGCA GCCCAACGCA CCTCGGAACA ACGTGCTGCG ATACTTTGAC
    TACGTTTTTA CAGGCGTCTT TACCTTTGAG ATGGTGATCA AGATGATTGA CCTGGGGCTC
    GTCCTGCATC AGGGTGCCTA CTTCCGTGAC CTCTGGAATA TTCTCGACTT CATAGTGGTC
    AGTGGGGCCC TGGTAGCCTT TGCCTTCACT GGCAATAGCA AAGGAAAAGA CATCAACACG
    ATTAAATCCC TCCGAGTCCT CCGGGTGCTA CGACCTCTTA AAACCATCAA GCGGCTGCCA
    AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG AACTCACTTA AAAACGTCTT CAACATCCTC
    ATCGTCTACA TGCTATTCAT GTTCATCTTC GCCGTGGTGG CTGTGCAGCT CTTCAAGGGG
    AAATTCTTCC ACTGCACTGA CGAGTCCAAA GAGTTTGAGA AAGATTGTCG AGGCAAATAC
    CTCCTCTACG AGAAGAATGA GGTGAAGGCG CGAGACCGGG AGTGGAAGAA GTATGAATTC
    CATTACGACA ATGTGCTGTG GGCTCTGCTG ACCCTCTTCA CCGTGTCCAC GGGAGAAGGC
    TGGCCACAGG TCCTCAAGCA TTCGGTGGAC GCCACCTTTG AGAACCAGGG CCCCAGCCCC
    GGGTACCGCA TGGAGATGTC CATTTTCTAC GTCGTCTACT TTGTGGTGTT CCCCTTCTTC
    TTTGTCAATA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAAGG GGACAAGATG
    ATGGAGGAAT ACAGCCTGGA GAAAAATGAG AGGGCCTGCA TTGATTTCGC CATCAGCGCC
    AAGCCGCTGA CCCGACACAT GCCGCAGAAC AAGCAGAGCT TCCAGTACCG CATGTGGCAG
    TTCGTGGTGT CTCCGCCTTT CGAGTACACG ATCATGGCCA TGATCGCCCT CAACACCATC
    GTGCTTATGA TGAAGTTCTA TGGGGCTTCT GTTGCTTATG AAAATGCCCT GCGGGTGTTC
    AACATCGTCT TCACCTCCCT CTTCTCTCTG GAATGTGTGC TGAAAGTCAT GGCTTTTGGG
    ATTCTGAATT ATTTCCGCGA TGCCTGGAAC ATCTTCGACT TTGTGACTGT TCTGGGCAGC
    ATCACCGATA TCCTCGTGAC TGAGTTTGGG AATAACTTCA TCAACCTGAG CTTTCTCCGC
    CTCTTCCGAG CTGCCCGGCT CATCAAACTT CTCCGTCAGG GTTACACCAT CCGCATTCTT
    CTCTGGACCT TTGTGCAGTC CTTCAAGGCC CTGCCTTATG TCTGTCTGCT GATCGCCATG
    CTCTTCTTCA TCTATGCCAT CATTGGGATG CAGGTGTTTG GTAACATTGG CATCGACGTG
    GAGGACGAGG ACAGTGATGA AGATGAGTTC CAAATCACTG AGCACAATAA CTTCCGGACC
    TTCTTCCAGG CCCTCATGCT TCTCTTCCGG AGTGCCACCG GGGAAGCTTG GCACAACATC
    ATGCTTTCCT GCCTCAGCGG GAAACCGTGT GATAAGAACT CTGGCATCCT GACTCGAGAG
    TGTGGCAATG AATTTGCTTA TTTTTACTTT GTTTCCTTCA TCTTCCTCTG CTCGTTTCTG
    ATGCTGAATC TCTTTGTCGC CGTCATCATG GACAACTTTG AGTACCTCAC CCGAGACTCC
    TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCCGA GTATGACCCC
    GCAGCTTGGG GCCGCATGCC TTACCTGGAC ATGTATCAGA TGCTGAGACA CATGTCTCCG
    CCCCTGGGTC TGGGGAAGAA GTGTCCGGCC AGAGTGGCTT ACAAGCGGCT TCTGCGGATG
    GACCTGCCCG TCGCAGATGA CAACACCGTC CACTTCAATT CCACCCTCAT GGCTCTGATC
    CGCACAGCCC TGGACATCAA GATTGCCAAG GGAGGAGCCG ACAAACAGCA GATGGACGCT
    GAGCTGCGGA AGGAGATGAT GGCGATTTGG CCCAATCTGT CCCAGAAGAC GCTAGACCTG
    CTGGTCACAC CTCACAAGTC CACGGACCTC ACCGTGGGGA AGATCTACGC AGCCATGATG
    ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG CGAGGAGCAG
    GACCGGACAC CCCTCATGTT CCAGCGCATG GAGCCCCCGT CCCCAACGCA GGAAGGGGGA
    CCTGGCCAGA ACGCCCTCCC CTCCACCCAG CTGGACCCAG GAGGAGCCCT GATGGCTCAC
    GAAAGCGGCC TCAAGGAGAG CCCGTCCTGG GTGACCCAGC GTGCCCAGGA GATGTTCCAG
    AAGACGGGCA CATGGAGTCC GGAACAAGGC CCCCCTACCG ACATGCCCAA CAGCCAGCCT
    AACTCTCAGT CCGTGGAGAT GCGAGAGATG GGCAGAGATG GCTACTCCGA CAGCGAGCAC
    TACCTCCCCA TGGAAGGCCA GGGCCGGGCT GCCTCCATGC CCCGCCTCCC TGCAGAGAAC
    CAGAGGAGAA GGGGCCGGCC ACGTGGGAAT AACCTCAGTA CCATCTCAGA CACCAGCCCC
    ATGAAGCGTT CAGCCTCCGT GCTGGGCCCC AAGGCCCGAC GCCTGGACGA TTACTCGCTG
    GAGCGGGTCC CGCCCGAGGA GAACCAGCGG CACCACCAGC GGCGCCGCGA CCGCAGCCAC
    CGCGCCTCTG AGCGCTCCCT GGGCCGCTAC ACCGATGTGG ACACAGGCTT GGGGACAGAC
    CTGAGCATGA CCACCCAATC CGGGGACCTG CCGTCGAAGG AGCGGGACCA GGAGCGGGGC
    CGGCCCAAGG ATCGGAAGCA TCGACAGCAC CACCACCACC ACCACCACCA CCACCATCCC
    CCGCCCCCCG ACAAGGACCG CTATGCCCAG GAACGGCCGG ACCACGGCCG GGCACGGGCT
    CGGGACCAGC GCTGGTCCCG CTCGCCCAGC GAGGGCCGAG AGCACATGGC GCACCGGCAG
    GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG
    CGGGGCCGCC GCCAGCTCCC CCAGACCCCC TCCACCCCCC GGCCACACGT GTCCTATTCC
    CCTGTGATCC GTAAGGCCGG CGGCTCGGGG CCCCCGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGCGGT GGCCAGGCCG GGCCGGGCGG CCACCAGCGG CCCTCGGAGG
    TACCCAGGCC CCACGGCCGA GCCTCTGGCC GGAGATCGGC CGCCCACGGG GGGCCACAGC
    AGCGGCCGCT CGCCCAGGAT GGAGAGGCGG GTCCCAGGCC CGGCCCGGAG CGAGTCCCCC
    AGGGCCTGTC GACACGGCGG GGCCCGGTGG CCGGCATCTG GCCCGCACGT GTCCGAGGGG
    CCCCCGGGTC CCCGGCACCA TGGCTACTAC CGGGGCTCCG ACTACGACGA GGCCGATGGC
    CCGGGCAGCG GGGGCGGCGA GGAGGCCATG GCCGGGGCCT ACGACGCGCC ACCCCCCGTA
    CGACACGCGT CCTCGGGCGC CACCGGGCGC TCGCCCAGGA CTCCCCGGGC CTCGGGCCCG
    GCCTGCGCCT CGCCTTCTCG GCACGGCCGG CGACTCCCCA ACGGCTACTA CCCGGCGCAC
    GGACTGGCCA GGCCCCGCGG GCCGGGCTCC AGGAAGGGCC TGCACGAACC CTACAGCGAG
    AGTGACGATG ATTGGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26122
    Clone ID Related Accession (Same CDS sequence) NM_001127222.1 , NM_001127222.2
    Accession Version NM_001127222.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008540.6, AF004883.1, AB035727.2, AC022436.6, AC124224.1, AC026805.5 and AC011446.6. On Jun 2, 2019 this sequence version replaced NM_001127222.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites and lacks an alternate in-frame exon, compared to variant 2. The resulting isoform (4) differs at three internal regions, compared to isoform 2, and includes the polyglutamine tract near the C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035727.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGCTCTG
    CGGAGAACCA CCATAAAGAA AAGCAAGACA GATTTGCTCA ACCCCGAAGA GGCTGAGGAT
    CAGCTGGCTG ATATAGCCTC TGTGGGTTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCGACCTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTACTCA GTTTGGTAGC TCTCAACACG
    CTGTGTGTTG CTATTGTTCA CTACAACCAG CCCGAGTGGC TCTCCGACTT CCTTTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGCTTTG ACTGTGGGGT TATCATTGGG
    AGCATCTTCG AGGTCATCTG GGCTGTCATA AAACCTGGCA CATCCTTTGG AATCAGCGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGA
    AACCTGGTCG TCTCTCTCCT CAACTCCATG AAGTCCATCA TCAGCCTGTT GTTTCTCCTT
    TTCCTGTTCA TTGTCGTCTT CGCCCTTTTG GGAATGCAAC TCTTCGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC CACCAACTTC GATACTTTTC CAGCAGCAAT AATGACGGTG
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ACGACGGGAT CAAGTCTCAG
    GGGGGCGTGC AGGGCGGCAT GGTGTTCTCC ATCTATTTCA TTGTACTGAC GCTCTTTGGG
    AACTACACCC TCCTGAATGT GTTCTTGGCC ATCGCTGTGG ACAATCTGGC CAACGCCCAG
    GAGCTCACCA AGGACGAGCA AGAGGAAGAA GAAGCAGCGA ACCAGAAACT TGCCCTACAG
    AAAGCCAAGG AGGTGGCAGA AGTGAGTCCT CTGTCCGCGG CCAACATGTC TATAGCTGTG
    AAAGAGCAAC AGAAGAATCA AAAGCCAGCC AAGTCCGTGT GGGAGCAGCG GACCAGTGAG
    ATGCGAAAGC AGAACTTGCT GGCCAGCCGG GAGGCCCTGT ATAACGAAAT GGACCCGGAC
    GAGCGCTGGA AGGCTGCCTA CACGCGGCAC CTGCGGCCAG ACATGAAGAC GCACTTGGAC
    CGGCCGCTGG TGGTGGACCC GCAGGAGAAC CGCAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CCGTGGACCA GCGCCTCGGC CAGCAGCGCG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGATCG GGCCCGGGAC CCCAGCGGCT CGGCGGGCCT GGACGCACGG
    AGGCCCTGGG CGGGAAGCCA GGAGGCCGAG CTGAGCCGGG AGGGACCCTA CGGCCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCAGCCTG GAGCAACCCG GGTTCTGGGA GGGCGAGGCC
    GAGCGAGGCA AGGCCGGGGA CCCCCACCGG AGGCACGTGC ACCGGCAGGG GGGCAGCAGG
    GAGAGCCGCA GCGGGTCCCC GCGCACGGGC GCGGACGGGG AGCATCGACG TCATCGCGCG
    CACCGCAGGC CCGGGGAGGA GGGTCCGGAG GACAAGGCGG AGCGGAGGGC GCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGGGCG AGGGCCCCGA CGGGGGCGAG
    CGCAGGAGAA GGCACCGGCA TGGCGCTCCA GCCACGTACG AGGGGGACGC GCGGAGGGAG
    GACAAGGAGC GGAGGCATCG GAGGAGGAAA GAGAACCAGG GCTCCGGGGT CCCTGTGTCG
    GGCCCCAACC TGTCAACCAC CCGGCCAATC CAGCAGGACC TGGGCCGCCA AGACCCACCC
    CTGGCAGAGG ATATTGACAA CATGAAGAAC AACAAGCTGG CCACCGCGGA GTCGGCCGCT
    CCCCACGGCA GCCTTGGCCA CGCCGGCCTG CCCCAGAGCC CAGCCAAGAT GGGAAACAGC
    ACCGACCCCG GCCCCATGCT GGCCATCCCT GCCATGGCCA CCAACCCCCA GAACGCCGCC
    AGCCGCCGGA CGCCCAACAA CCCGGGGAAC CCATCCAATC CCGGCCCCCC CAAGACCCCC
    GAGAATAGCC TTATCGTCAC CAACCCCAGC GGCACCCAGA CCAATTCAGC TAAGACTGCC
    AGGAAACCCG ACCACACCAC AGTGGACATC CCCCCAGCCT GCCCACCCCC CCTCAACCAC
    ACCGTCGTAC AAGTGAACAA AAACGCCAAC CCAGACCCAC TGCCAAAAAA AGAGGAAGAG
    AAGAAGGAGG AGGAGGAAGA CGACCGTGGG GAAGACGGCC CTAAGCCAAT GCCTCCCTAT
    AGCTCCATGT TCATCCTGTC CACGACCAAC CCCCTTCGCC GCCTGTGCCA TTACATCCTG
    AACCTGCGCT ACTTTGAGAT GTGCATCCTC ATGGTCATTG CCATGAGCAG CATCGCCCTG
    GCCGCCGAGG ACCCTGTGCA GCCCAACGCA CCTCGGAACA ACGTGCTGCG ATACTTTGAC
    TACGTTTTTA CAGGCGTCTT TACCTTTGAG ATGGTGATCA AGATGATTGA CCTGGGGCTC
    GTCCTGCATC AGGGTGCCTA CTTCCGTGAC CTCTGGAATA TTCTCGACTT CATAGTGGTC
    AGTGGGGCCC TGGTAGCCTT TGCCTTCACT GGCAATAGCA AAGGAAAAGA CATCAACACG
    ATTAAATCCC TCCGAGTCCT CCGGGTGCTA CGACCTCTTA AAACCATCAA GCGGCTGCCA
    AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG AACTCACTTA AAAACGTCTT CAACATCCTC
    ATCGTCTACA TGCTATTCAT GTTCATCTTC GCCGTGGTGG CTGTGCAGCT CTTCAAGGGG
    AAATTCTTCC ACTGCACTGA CGAGTCCAAA GAGTTTGAGA AAGATTGTCG AGGCAAATAC
    CTCCTCTACG AGAAGAATGA GGTGAAGGCG CGAGACCGGG AGTGGAAGAA GTATGAATTC
    CATTACGACA ATGTGCTGTG GGCTCTGCTG ACCCTCTTCA CCGTGTCCAC GGGAGAAGGC
    TGGCCACAGG TCCTCAAGCA TTCGGTGGAC GCCACCTTTG AGAACCAGGG CCCCAGCCCC
    GGGTACCGCA TGGAGATGTC CATTTTCTAC GTCGTCTACT TTGTGGTGTT CCCCTTCTTC
    TTTGTCAATA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAAGG GGACAAGATG
    ATGGAGGAAT ACAGCCTGGA GAAAAATGAG AGGGCCTGCA TTGATTTCGC CATCAGCGCC
    AAGCCGCTGA CCCGACACAT GCCGCAGAAC AAGCAGAGCT TCCAGTACCG CATGTGGCAG
    TTCGTGGTGT CTCCGCCTTT CGAGTACACG ATCATGGCCA TGATCGCCCT CAACACCATC
    GTGCTTATGA TGAAGTTCTA TGGGGCTTCT GTTGCTTATG AAAATGCCCT GCGGGTGTTC
    AACATCGTCT TCACCTCCCT CTTCTCTCTG GAATGTGTGC TGAAAGTCAT GGCTTTTGGG
    ATTCTGAATT ATTTCCGCGA TGCCTGGAAC ATCTTCGACT TTGTGACTGT TCTGGGCAGC
    ATCACCGATA TCCTCGTGAC TGAGTTTGGG AATAACTTCA TCAACCTGAG CTTTCTCCGC
    CTCTTCCGAG CTGCCCGGCT CATCAAACTT CTCCGTCAGG GTTACACCAT CCGCATTCTT
    CTCTGGACCT TTGTGCAGTC CTTCAAGGCC CTGCCTTATG TCTGTCTGCT GATCGCCATG
    CTCTTCTTCA TCTATGCCAT CATTGGGATG CAGGTGTTTG GTAACATTGG CATCGACGTG
    GAGGACGAGG ACAGTGATGA AGATGAGTTC CAAATCACTG AGCACAATAA CTTCCGGACC
    TTCTTCCAGG CCCTCATGCT TCTCTTCCGG AGTGCCACCG GGGAAGCTTG GCACAACATC
    ATGCTTTCCT GCCTCAGCGG GAAACCGTGT GATAAGAACT CTGGCATCCT GACTCGAGAG
    TGTGGCAATG AATTTGCTTA TTTTTACTTT GTTTCCTTCA TCTTCCTCTG CTCGTTTCTG
    ATGCTGAATC TCTTTGTCGC CGTCATCATG GACAACTTTG AGTACCTCAC CCGAGACTCC
    TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCCGA GTATGACCCC
    GCAGCTTGGG GCCGCATGCC TTACCTGGAC ATGTATCAGA TGCTGAGACA CATGTCTCCG
    CCCCTGGGTC TGGGGAAGAA GTGTCCGGCC AGAGTGGCTT ACAAGCGGCT TCTGCGGATG
    GACCTGCCCG TCGCAGATGA CAACACCGTC CACTTCAATT CCACCCTCAT GGCTCTGATC
    CGCACAGCCC TGGACATCAA GATTGCCAAG GGAGGAGCCG ACAAACAGCA GATGGACGCT
    GAGCTGCGGA AGGAGATGAT GGCGATTTGG CCCAATCTGT CCCAGAAGAC GCTAGACCTG
    CTGGTCACAC CTCACAAGTC CACGGACCTC ACCGTGGGGA AGATCTACGC AGCCATGATG
    ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG CGAGGAGCAG
    GACCGGACAC CCCTCATGTT CCAGCGCATG GAGCCCCCGT CCCCAACGCA GGAAGGGGGA
    CCTGGCCAGA ACGCCCTCCC CTCCACCCAG CTGGACCCAG GAGGAGCCCT GATGGCTCAC
    GAAAGCGGCC TCAAGGAGAG CCCGTCCTGG GTGACCCAGC GTGCCCAGGA GATGTTCCAG
    AAGACGGGCA CATGGAGTCC GGAACAAGGC CCCCCTACCG ACATGCCCAA CAGCCAGCCT
    AACTCTCAGT CCGTGGAGAT GCGAGAGATG GGCAGAGATG GCTACTCCGA CAGCGAGCAC
    TACCTCCCCA TGGAAGGCCA GGGCCGGGCT GCCTCCATGC CCCGCCTCCC TGCAGAGAAC
    CAGAGGAGAA GGGGCCGGCC ACGTGGGAAT AACCTCAGTA CCATCTCAGA CACCAGCCCC
    ATGAAGCGTT CAGCCTCCGT GCTGGGCCCC AAGGCCCGAC GCCTGGACGA TTACTCGCTG
    GAGCGGGTCC CGCCCGAGGA GAACCAGCGG CACCACCAGC GGCGCCGCGA CCGCAGCCAC
    CGCGCCTCTG AGCGCTCCCT GGGCCGCTAC ACCGATGTGG ACACAGGCTT GGGGACAGAC
    CTGAGCATGA CCACCCAATC CGGGGACCTG CCGTCGAAGG AGCGGGACCA GGAGCGGGGC
    CGGCCCAAGG ATCGGAAGCA TCGACAGCAC CACCACCACC ACCACCACCA CCACCATCCC
    CCGCCCCCCG ACAAGGACCG CTATGCCCAG GAACGGCCGG ACCACGGCCG GGCACGGGCT
    CGGGACCAGC GCTGGTCCCG CTCGCCCAGC GAGGGCCGAG AGCACATGGC GCACCGGCAG
    GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG
    CGGGGCCGCC GCCAGCTCCC CCAGACCCCC TCCACCCCCC GGCCACACGT GTCCTATTCC
    CCTGTGATCC GTAAGGCCGG CGGCTCGGGG CCCCCGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGCGGT GGCCAGGCCG GGCCGGGCGG CCACCAGCGG CCCTCGGAGG
    TACCCAGGCC CCACGGCCGA GCCTCTGGCC GGAGATCGGC CGCCCACGGG GGGCCACAGC
    AGCGGCCGCT CGCCCAGGAT GGAGAGGCGG GTCCCAGGCC CGGCCCGGAG CGAGTCCCCC
    AGGGCCTGTC GACACGGCGG GGCCCGGTGG CCGGCATCTG GCCCGCACGT GTCCGAGGGG
    CCCCCGGGTC CCCGGCACCA TGGCTACTAC CGGGGCTCCG ACTACGACGA GGCCGATGGC
    CCGGGCAGCG GGGGCGGCGA GGAGGCCATG GCCGGGGCCT ACGACGCGCC ACCCCCCGTA
    CGACACGCGT CCTCGGGCGC CACCGGGCGC TCGCCCAGGA CTCCCCGGGC CTCGGGCCCG
    GCCTGCGCCT CGCCTTCTCG GCACGGCCGG CGACTCCCCA ACGGCTACTA CCCGGCGCAC
    GGACTGGCCA GGCCCCGCGG GCCGGGCTCC AGGAAGGGCC TGCACGAACC CTACAGCGAG
    AGTGACGATG ATTGGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120694.1
    CDS256..7776
    Translation

    Target ORF information:

    RefSeq Version NM_001127222.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127222.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGCTCTG
    CGGAGAACCA CCATAAAGAA AAGCAAGACA GATTTGCTCA ACCCCGAAGA GGCTGAGGAT
    CAGCTGGCTG ATATAGCCTC TGTGGGTTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCGACCTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTACTCA GTTTGGTAGC TCTCAACACG
    CTGTGTGTTG CTATTGTTCA CTACAACCAG CCCGAGTGGC TCTCCGACTT CCTTTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGCTTTG ACTGTGGGGT TATCATTGGG
    AGCATCTTCG AGGTCATCTG GGCTGTCATA AAACCTGGCA CATCCTTTGG AATCAGCGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGA
    AACCTGGTCG TCTCTCTCCT CAACTCCATG AAGTCCATCA TCAGCCTGTT GTTTCTCCTT
    TTCCTGTTCA TTGTCGTCTT CGCCCTTTTG GGAATGCAAC TCTTCGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC CACCAACTTC GATACTTTTC CAGCAGCAAT AATGACGGTG
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ACGACGGGAT CAAGTCTCAG
    GGGGGCGTGC AGGGCGGCAT GGTGTTCTCC ATCTATTTCA TTGTACTGAC GCTCTTTGGG
    AACTACACCC TCCTGAATGT GTTCTTGGCC ATCGCTGTGG ACAATCTGGC CAACGCCCAG
    GAGCTCACCA AGGACGAGCA AGAGGAAGAA GAAGCAGCGA ACCAGAAACT TGCCCTACAG
    AAAGCCAAGG AGGTGGCAGA AGTGAGTCCT CTGTCCGCGG CCAACATGTC TATAGCTGTG
    AAAGAGCAAC AGAAGAATCA AAAGCCAGCC AAGTCCGTGT GGGAGCAGCG GACCAGTGAG
    ATGCGAAAGC AGAACTTGCT GGCCAGCCGG GAGGCCCTGT ATAACGAAAT GGACCCGGAC
    GAGCGCTGGA AGGCTGCCTA CACGCGGCAC CTGCGGCCAG ACATGAAGAC GCACTTGGAC
    CGGCCGCTGG TGGTGGACCC GCAGGAGAAC CGCAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CCGTGGACCA GCGCCTCGGC CAGCAGCGCG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGATCG GGCCCGGGAC CCCAGCGGCT CGGCGGGCCT GGACGCACGG
    AGGCCCTGGG CGGGAAGCCA GGAGGCCGAG CTGAGCCGGG AGGGACCCTA CGGCCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCAGCCTG GAGCAACCCG GGTTCTGGGA GGGCGAGGCC
    GAGCGAGGCA AGGCCGGGGA CCCCCACCGG AGGCACGTGC ACCGGCAGGG GGGCAGCAGG
    GAGAGCCGCA GCGGGTCCCC GCGCACGGGC GCGGACGGGG AGCATCGACG TCATCGCGCG
    CACCGCAGGC CCGGGGAGGA GGGTCCGGAG GACAAGGCGG AGCGGAGGGC GCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGGGCG AGGGCCCCGA CGGGGGCGAG
    CGCAGGAGAA GGCACCGGCA TGGCGCTCCA GCCACGTACG AGGGGGACGC GCGGAGGGAG
    GACAAGGAGC GGAGGCATCG GAGGAGGAAA GAGAACCAGG GCTCCGGGGT CCCTGTGTCG
    GGCCCCAACC TGTCAACCAC CCGGCCAATC CAGCAGGACC TGGGCCGCCA AGACCCACCC
    CTGGCAGAGG ATATTGACAA CATGAAGAAC AACAAGCTGG CCACCGCGGA GTCGGCCGCT
    CCCCACGGCA GCCTTGGCCA CGCCGGCCTG CCCCAGAGCC CAGCCAAGAT GGGAAACAGC
    ACCGACCCCG GCCCCATGCT GGCCATCCCT GCCATGGCCA CCAACCCCCA GAACGCCGCC
    AGCCGCCGGA CGCCCAACAA CCCGGGGAAC CCATCCAATC CCGGCCCCCC CAAGACCCCC
    GAGAATAGCC TTATCGTCAC CAACCCCAGC GGCACCCAGA CCAATTCAGC TAAGACTGCC
    AGGAAACCCG ACCACACCAC AGTGGACATC CCCCCAGCCT GCCCACCCCC CCTCAACCAC
    ACCGTCGTAC AAGTGAACAA AAACGCCAAC CCAGACCCAC TGCCAAAAAA AGAGGAAGAG
    AAGAAGGAGG AGGAGGAAGA CGACCGTGGG GAAGACGGCC CTAAGCCAAT GCCTCCCTAT
    AGCTCCATGT TCATCCTGTC CACGACCAAC CCCCTTCGCC GCCTGTGCCA TTACATCCTG
    AACCTGCGCT ACTTTGAGAT GTGCATCCTC ATGGTCATTG CCATGAGCAG CATCGCCCTG
    GCCGCCGAGG ACCCTGTGCA GCCCAACGCA CCTCGGAACA ACGTGCTGCG ATACTTTGAC
    TACGTTTTTA CAGGCGTCTT TACCTTTGAG ATGGTGATCA AGATGATTGA CCTGGGGCTC
    GTCCTGCATC AGGGTGCCTA CTTCCGTGAC CTCTGGAATA TTCTCGACTT CATAGTGGTC
    AGTGGGGCCC TGGTAGCCTT TGCCTTCACT GGCAATAGCA AAGGAAAAGA CATCAACACG
    ATTAAATCCC TCCGAGTCCT CCGGGTGCTA CGACCTCTTA AAACCATCAA GCGGCTGCCA
    AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG AACTCACTTA AAAACGTCTT CAACATCCTC
    ATCGTCTACA TGCTATTCAT GTTCATCTTC GCCGTGGTGG CTGTGCAGCT CTTCAAGGGG
    AAATTCTTCC ACTGCACTGA CGAGTCCAAA GAGTTTGAGA AAGATTGTCG AGGCAAATAC
    CTCCTCTACG AGAAGAATGA GGTGAAGGCG CGAGACCGGG AGTGGAAGAA GTATGAATTC
    CATTACGACA ATGTGCTGTG GGCTCTGCTG ACCCTCTTCA CCGTGTCCAC GGGAGAAGGC
    TGGCCACAGG TCCTCAAGCA TTCGGTGGAC GCCACCTTTG AGAACCAGGG CCCCAGCCCC
    GGGTACCGCA TGGAGATGTC CATTTTCTAC GTCGTCTACT TTGTGGTGTT CCCCTTCTTC
    TTTGTCAATA TCTTTGTGGC CTTGATCATC ATCACCTTCC AGGAGCAAGG GGACAAGATG
    ATGGAGGAAT ACAGCCTGGA GAAAAATGAG AGGGCCTGCA TTGATTTCGC CATCAGCGCC
    AAGCCGCTGA CCCGACACAT GCCGCAGAAC AAGCAGAGCT TCCAGTACCG CATGTGGCAG
    TTCGTGGTGT CTCCGCCTTT CGAGTACACG ATCATGGCCA TGATCGCCCT CAACACCATC
    GTGCTTATGA TGAAGTTCTA TGGGGCTTCT GTTGCTTATG AAAATGCCCT GCGGGTGTTC
    AACATCGTCT TCACCTCCCT CTTCTCTCTG GAATGTGTGC TGAAAGTCAT GGCTTTTGGG
    ATTCTGAATT ATTTCCGCGA TGCCTGGAAC ATCTTCGACT TTGTGACTGT TCTGGGCAGC
    ATCACCGATA TCCTCGTGAC TGAGTTTGGG AATAACTTCA TCAACCTGAG CTTTCTCCGC
    CTCTTCCGAG CTGCCCGGCT CATCAAACTT CTCCGTCAGG GTTACACCAT CCGCATTCTT
    CTCTGGACCT TTGTGCAGTC CTTCAAGGCC CTGCCTTATG TCTGTCTGCT GATCGCCATG
    CTCTTCTTCA TCTATGCCAT CATTGGGATG CAGGTGTTTG GTAACATTGG CATCGACGTG
    GAGGACGAGG ACAGTGATGA AGATGAGTTC CAAATCACTG AGCACAATAA CTTCCGGACC
    TTCTTCCAGG CCCTCATGCT TCTCTTCCGG AGTGCCACCG GGGAAGCTTG GCACAACATC
    ATGCTTTCCT GCCTCAGCGG GAAACCGTGT GATAAGAACT CTGGCATCCT GACTCGAGAG
    TGTGGCAATG AATTTGCTTA TTTTTACTTT GTTTCCTTCA TCTTCCTCTG CTCGTTTCTG
    ATGCTGAATC TCTTTGTCGC CGTCATCATG GACAACTTTG AGTACCTCAC CCGAGACTCC
    TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCCGA GTATGACCCC
    GCAGCTTGGG GCCGCATGCC TTACCTGGAC ATGTATCAGA TGCTGAGACA CATGTCTCCG
    CCCCTGGGTC TGGGGAAGAA GTGTCCGGCC AGAGTGGCTT ACAAGCGGCT TCTGCGGATG
    GACCTGCCCG TCGCAGATGA CAACACCGTC CACTTCAATT CCACCCTCAT GGCTCTGATC
    CGCACAGCCC TGGACATCAA GATTGCCAAG GGAGGAGCCG ACAAACAGCA GATGGACGCT
    GAGCTGCGGA AGGAGATGAT GGCGATTTGG CCCAATCTGT CCCAGAAGAC GCTAGACCTG
    CTGGTCACAC CTCACAAGTC CACGGACCTC ACCGTGGGGA AGATCTACGC AGCCATGATG
    ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG CGAGGAGCAG
    GACCGGACAC CCCTCATGTT CCAGCGCATG GAGCCCCCGT CCCCAACGCA GGAAGGGGGA
    CCTGGCCAGA ACGCCCTCCC CTCCACCCAG CTGGACCCAG GAGGAGCCCT GATGGCTCAC
    GAAAGCGGCC TCAAGGAGAG CCCGTCCTGG GTGACCCAGC GTGCCCAGGA GATGTTCCAG
    AAGACGGGCA CATGGAGTCC GGAACAAGGC CCCCCTACCG ACATGCCCAA CAGCCAGCCT
    AACTCTCAGT CCGTGGAGAT GCGAGAGATG GGCAGAGATG GCTACTCCGA CAGCGAGCAC
    TACCTCCCCA TGGAAGGCCA GGGCCGGGCT GCCTCCATGC CCCGCCTCCC TGCAGAGAAC
    CAGAGGAGAA GGGGCCGGCC ACGTGGGAAT AACCTCAGTA CCATCTCAGA CACCAGCCCC
    ATGAAGCGTT CAGCCTCCGT GCTGGGCCCC AAGGCCCGAC GCCTGGACGA TTACTCGCTG
    GAGCGGGTCC CGCCCGAGGA GAACCAGCGG CACCACCAGC GGCGCCGCGA CCGCAGCCAC
    CGCGCCTCTG AGCGCTCCCT GGGCCGCTAC ACCGATGTGG ACACAGGCTT GGGGACAGAC
    CTGAGCATGA CCACCCAATC CGGGGACCTG CCGTCGAAGG AGCGGGACCA GGAGCGGGGC
    CGGCCCAAGG ATCGGAAGCA TCGACAGCAC CACCACCACC ACCACCACCA CCACCATCCC
    CCGCCCCCCG ACAAGGACCG CTATGCCCAG GAACGGCCGG ACCACGGCCG GGCACGGGCT
    CGGGACCAGC GCTGGTCCCG CTCGCCCAGC GAGGGCCGAG AGCACATGGC GCACCGGCAG
    GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG
    CGGGGCCGCC GCCAGCTCCC CCAGACCCCC TCCACCCCCC GGCCACACGT GTCCTATTCC
    CCTGTGATCC GTAAGGCCGG CGGCTCGGGG CCCCCGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGCGGT GGCCAGGCCG GGCCGGGCGG CCACCAGCGG CCCTCGGAGG
    TACCCAGGCC CCACGGCCGA GCCTCTGGCC GGAGATCGGC CGCCCACGGG GGGCCACAGC
    AGCGGCCGCT CGCCCAGGAT GGAGAGGCGG GTCCCAGGCC CGGCCCGGAG CGAGTCCCCC
    AGGGCCTGTC GACACGGCGG GGCCCGGTGG CCGGCATCTG GCCCGCACGT GTCCGAGGGG
    CCCCCGGGTC CCCGGCACCA TGGCTACTAC CGGGGCTCCG ACTACGACGA GGCCGATGGC
    CCGGGCAGCG GGGGCGGCGA GGAGGCCATG GCCGGGGCCT ACGACGCGCC ACCCCCCGTA
    CGACACGCGT CCTCGGGCGC CACCGGGCGC TCGCCCAGGA CTCCCCGGGC CTCGGGCCCG
    GCCTGCGCCT CGCCTTCTCG GCACGGCCGG CGACTCCCCA ACGGCTACTA CCCGGCGCAC
    GGACTGGCCA GGCCCCGCGG GCCGGGCTCC AGGAAGGGCC TGCACGAACC CTACAGCGAG
    AGTGACGATG ATTGGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26360
    Clone ID Related Accession (Same CDS sequence) NM_001127221.1
    Accession Version NM_001127221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Homo sapiens(human)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF004883.1, X99897.1, AC124224.1 and AC011446.6. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) uses two alternate splice sites, lacks two alternate exons, and includes an additional exon, compared to variant 2. The resulting isoform (3) differs at 3 internal regions and has a shorter and distinct C-terminus that does not include the polyglutamine tract, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X99897.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGACGA GCAAGAGGAA GAAGAAGCAG CGAACCAGAA ACTTGCCCTA
    CAGAAAGCCA AGGAGGTGGC AGAAGTGAGT CCTCTGTCCG CGGCCAACAT GTCTATAGCT
    GTGAAAGAGC AACAGAAGAA TCAAAAGCCA GCCAAGTCCG TGTGGGAGCA GCGGACCAGT
    GAGATGCGAA AGCAGAACTT GCTGGCCAGC CGGGAGGCCC TGTATAACGA AATGGACCCG
    GACGAGCGCT GGAAGGCTGC CTACACGCGG CACCTGCGGC CAGACATGAA GACGCACTTG
    GACCGGCCGC TGGTGGTGGA CCCGCAGGAG AACCGCAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACCGTGGA CCAGCGCCTC GGCCAGCAGC GCGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA TCGGGCCCGG GACCCCAGCG GCTCGGCGGG CCTGGACGCA
    CGGAGGCCCT GGGCGGGAAG CCAGGAGGCC GAGCTGAGCC GGGAGGGACC CTACGGCCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCAGC CTGGAGCAAC CCGGGTTCTG GGAGGGCGAG
    GCCGAGCGAG GCAAGGCCGG GGACCCCCAC CGGAGGCACG TGCACCGGCA GGGGGGCAGC
    AGGGAGAGCC GCAGCGGGTC CCCGCGCACG GGCGCGGACG GGGAGCATCG ACGTCATCGC
    GCGCACCGCA GGCCCGGGGA GGAGGGTCCG GAGGACAAGG CGGAGCGGAG GGCGCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGG GCGAGGGCCC CGACGGGGGC
    GAGCGCAGGA GAAGGCACCG GCATGGCGCT CCAGCCACGT ACGAGGGGGA CGCGCGGAGG
    GAGGACAAGG AGCGGAGGCA TCGGAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCTGTG
    TCGGGCCCCA ACCTGTCAAC CACCCGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCA
    CCCCTGGCAG AGGATATTGA CAACATGAAG AACAACAAGC TGGCCACCGC GGAGTCGGCC
    GCTCCCCACG GCAGCCTTGG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGAAAC
    AGCACCGACC CCGGCCCCAT GCTGGCCATC CCTGCCATGG CCACCAACCC CCAGAACGCC
    GCCAGCCGCC GGACGCCCAA CAACCCGGGG AACCCATCCA ATCCCGGCCC CCCCAAGACC
    CCCGAGAATA GCCTTATCGT CACCAACCCC AGCGGCACCC AGACCAATTC AGCTAAGACT
    GCCAGGAAAC CCGACCACAC CACAGTGGAC ATCCCCCCAG CCTGCCCACC CCCCCTCAAC
    CACACCGTCG TACAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAAGAGGAA
    GAGAAGAAGG AGGAGGAGGA AGACGACCGT GGGGAAGACG GCCCTAAGCC AATGCCTCCC
    TATAGCTCCA TGTTCATCCT GTCCACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATC CTCATGGTCA TTGCCATGAG CAGCATCGCC
    CTGGCCGCCG AGGACCCTGT GCAGCCCAAC GCACCTCGGA ACAACGTGCT GCGATACTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT TGACCTGGGG
    CTCGTCCTGC ATCAGGGTGC CTACTTCCGT GACCTCTGGA ATATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTAGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA AGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGCTG
    CCAAAGCTCA AGGCTGTGTT TGACTGTGTG GTGAACTCAC TTAAAAACGT CTTCAACATC
    CTCATCGTCT ACATGCTATT CATGTTCATC TTCGCCGTGG TGGCTGTGCA GCTCTTCAAG
    GGGAAATTCT TCCACTGCAC TGACGAGTCC AAAGAGTTTG AGAAAGATTG TCGAGGCAAA
    TACCTCCTCT ACGAGAAGAA TGAGGTGAAG GCGCGAGACC GGGAGTGGAA GAAGTATGAA
    TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCATTCGGTG GACGCCACCT TTGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATTTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ATATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AATACAGCCT GGAGAAAAAT GAGAGGGCCT GCATTGATTT CGCCATCAGC
    GCCAAGCCGC TGACCCGACA CATGCCGCAG AACAAGCAGA GCTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCTCCGCC TTTCGAGTAC ACGATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTTA TGATGAAGTT CTATGGGGCT TCTGTTGCTT ATGAAAATGC CCTGCGGGTG
    TTCAACATCG TCTTCACCTC CCTCTTCTCT CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTCCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ATATCCTCGT GACTGAGTTT GGGAATAACT TCATCAACCT GAGCTTTCTC
    CGCCTCTTCC GAGCTGCCCG GCTCATCAAA CTTCTCCGTC AGGGTTACAC CATCCGCATT
    CTTCTCTGGA CCTTTGTGCA GTCCTTCAAG GCCCTGCCTT ATGTCTGTCT GCTGATCGCC
    ATGCTCTTCT TCATCTATGC CATCATTGGG ATGCAGGTGT TTGGTAACAT TGGCATCGAC
    GTGGAGGACG AGGACAGTGA TGAAGATGAG TTCCAAATCA CTGAGCACAA TAACTTCCGG
    ACCTTCTTCC AGGCCCTCAT GCTTCTCTTC CGGAGTGCCA CCGGGGAAGC TTGGCACAAC
    ATCATGCTTT CCTGCCTCAG CGGGAAACCG TGTGATAAGA ACTCTGGCAT CCTGACTCGA
    GAGTGTGGCA ATGAATTTGC TTATTTTTAC TTTGTTTCCT TCATCTTCCT CTGCTCGTTT
    CTGATGCTGA ATCTCTTTGT CGCCGTCATC ATGGACAACT TTGAGTACCT CACCCGAGAC
    TCCTCCATCC TGGGCCCCCA CCACCTGGAT GAGTACGTGC GTGTCTGGGC CGAGTATGAC
    CCCGCAGCTT GCGGTCGGAT TCATTATAAG GATATGTACA GTTTATTACG AGTAATATCT
    CCCCCTCTCG GCTTAGGCAA GAAATGTCCT CATAGGGTTG CTTGCAAGCG GCTTCTGCGG
    ATGGACCTGC CCGTCGCAGA TGACAACACC GTCCACTTCA ATTCCACCCT CATGGCTCTG
    ATCCGCACAG CCCTGGACAT CAAGATTGCC AAGGGAGGAG CCGACAAACA GCAGATGGAC
    GCTGAGCTGC GGAAGGAGAT GATGGCGATT TGGCCCAATC TGTCCCAGAA GACGCTAGAC
    CTGCTGGTCA CACCTCACAA GTCCACGGAC CTCACCGTGG GGAAGATCTA CGCAGCCATG
    ATGATCATGG AGTACTACCG GCAGAGCAAG GCCAAGAAGC TGCAGGCCAT GCGCGAGGAG
    CAGGACCGGA CACCCCTCAT GTTCCAGCGC ATGGAGCCCC CGTCCCCAAC GCAGGAAGGG
    GGACCTGGCC AGAACGCCCT CCCCTCCACC CAGCTGGACC CAGGAGGAGC CCTGATGGCT
    CACGAAAGCG GCCTCAAGGA GAGCCCGTCC TGGGTGACCC AGCGTGCCCA GGAGATGTTC
    CAGAAGACGG GCACATGGAG TCCGGAACAA GGCCCCCCTA CCGACATGCC CAACAGCCAG
    CCTAACTCTC AGTCCGTGGA GATGCGAGAG ATGGGCAGAG ATGGCTACTC CGACAGCGAG
    CACTACCTCC CCATGGAAGG CCAGGGCCGG GCTGCCTCCA TGCCCCGCCT CCCTGCAGAG
    AACCAGAGGA GAAGGGGCCG GCCACGTGGG AATAACCTCA GTACCATCTC AGACACCAGC
    CCCATGAAGC GTTCAGCCTC CGTGCTGGGC CCCAAGGCCC GACGCCTGGA CGATTACTCG
    CTGGAGCGGG TCCCGCCCGA GGAGAACCAG CGGCACCACC AGCGGCGCCG CGACCGCAGC
    CACCGCGCCT CTGAGCGCTC CCTGGGCCGC TACACCGATG TGGACACAGG CTTGGGGACA
    GACCTGAGCA TGACCACCCA ATCCGGGGAC CTGCCGTCGA AGGAGCGGGA CCAGGAGCGG
    GGCCGGCCCA AGGATCGGAA GCATCGACAG CACCACCACC ACCACCACCA CCACCACCAT
    CCCCCGCCCC CCGACAAGGA CCGCTATGCC CAGGAACGGC CGGACCACGG CCGGGCACGG
    GCTCGGGACC AGCGCTGGTC CCGCTCGCCC AGCGAGGGCC GAGAGCACAT GGCGCACCGG
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120693.1
    CDS237..7022
    Translation

    Target ORF information:

    RefSeq Version NM_001127221.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCAG CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCA
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TTAACCGGTC TCTCTTCCTC
    TTCAGCGAAG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCG AATTGCATCG TCCTCGCACT GGAGCAGCAT
    CTGCCTGATG ATGACAAGAC CCCGATGTCT GAACGGCTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATTG CCCTTGGGTT TGCCTTCCAC
    AAAGGCTCCT ACTTGAGGAA TGGCTGGAAT GTCATGGACT TTGTGGTGGT GCTAACGGGC
    ATCTTGGCGA CAGTTGGGAC GGAGTTTGAC CTACGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCGATGA TCCCTTTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGG
    ACAGATGACA TTCAGGGTGA GTCTCCGGCT CCATGTGGGA CAGAAGAGCC CGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGGC CCAACAACGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATAACCAT GGAAGGGTGG
    ACTGATCTCC TCTACAATAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TTGTGCTGGG TGTGCTGTCA
    GGGGAGTTTG CCAAAGAAAG GGAACGGGTG GAGAACCGGC GGGCTTTTCT GAAGCTGAGG
    CGGCAACAAC AGATTGAACG TGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAGGA TGAAACTGAC GGGGAGCAGA GGCATCCCTT TGATGGAGCT
    CTGCGGAGAA CCACCATAAA GAAAAGCAAG ACAGATTTGC TCAACCCCGA AGAGGCTGAG
    GATCAGCTGG CTGATATAGC CTCTGTGGGT TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCGAC CTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTAC TCAGTTTGGT AGCTCTCAAC
    ACGCTGTGTG TTGCTATTGT TCACTACAAC CAGCCCGAGT GGCTCTCCGA CTTCCTTTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGCT TTGACTGTGG GGTTATCATT
    GGGAGCATCT TCGAGGTCAT CTGGGCTGTC ATAAAACCTG GCACATCCTT TGGAATCAGC
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGAAACCTGG TCGTCTCTCT CCTCAACTCC ATGAAGTCCA TCATCAGCCT GTTGTTTCTC
    CTTTTCCTGT TCATTGTCGT CTTCGCCCTT TTGGGAATGC AACTCTTCGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCCACCAAC TTCGATACTT TTCCAGCAGC AATAATGACG
    GTGTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTACGACGG GATCAAGTCT
    CAGGGGGGCG TGCAGGGCGG CATGGTGTTC TCCATCTATT TCATTGTACT GACGCTCTTT
    GGGAACTACA CCCTCCTGAA TGTGTTCTTG GCCATCGCTG TGGACAATCT GGCCAACGCC
    CAGGAGCTCA CCAAGGACGA GCAAGAGGAA GAAGAAGCAG CGAACCAGAA ACTTGCCCTA
    CAGAAAGCCA AGGAGGTGGC AGAAGTGAGT CCTCTGTCCG CGGCCAACAT GTCTATAGCT
    GTGAAAGAGC AACAGAAGAA TCAAAAGCCA GCCAAGTCCG TGTGGGAGCA GCGGACCAGT
    GAGATGCGAA AGCAGAACTT GCTGGCCAGC CGGGAGGCCC TGTATAACGA AATGGACCCG
    GACGAGCGCT GGAAGGCTGC CTACACGCGG CACCTGCGGC CAGACATGAA GACGCACTTG
    GACCGGCCGC TGGTGGTGGA CCCGCAGGAG AACCGCAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACCGTGGA CCAGCGCCTC GGCCAGCAGC GCGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA TCGGGCCCGG GACCCCAGCG GCTCGGCGGG CCTGGACGCA
    CGGAGGCCCT GGGCGGGAAG CCAGGAGGCC GAGCTGAGCC GGGAGGGACC CTACGGCCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCAGC CTGGAGCAAC CCGGGTTCTG GGAGGGCGAG
    GCCGAGCGAG GCAAGGCCGG GGACCCCCAC CGGAGGCACG TGCACCGGCA GGGGGGCAGC
    AGGGAGAGCC GCAGCGGGTC CCCGCGCACG GGCGCGGACG GGGAGCATCG ACGTCATCGC
    GCGCACCGCA GGCCCGGGGA GGAGGGTCCG GAGGACAAGG CGGAGCGGAG GGCGCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGG GCGAGGGCCC CGACGGGGGC
    GAGCGCAGGA GAAGGCACCG GCATGGCGCT CCAGCCACGT ACGAGGGGGA CGCGCGGAGG
    GAGGACAAGG AGCGGAGGCA TCGGAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCTGTG
    TCGGGCCCCA ACCTGTCAAC CACCCGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCA
    CCCCTGGCAG AGGATATTGA CAACATGAAG AACAACAAGC TGGCCACCGC GGAGTCGGCC
    GCTCCCCACG GCAGCCTTGG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGAAAC
    AGCACCGACC CCGGCCCCAT GCTGGCCATC CCTGCCATGG CCACCAACCC CCAGAACGCC
    GCCAGCCGCC GGACGCCCAA CAACCCGGGG AACCCATCCA ATCCCGGCCC CCCCAAGACC
    CCCGAGAATA GCCTTATCGT CACCAACCCC AGCGGCACCC AGACCAATTC AGCTAAGACT
    GCCAGGAAAC CCGACCACAC CACAGTGGAC ATCCCCCCAG CCTGCCCACC CCCCCTCAAC
    CACACCGTCG TACAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAAGAGGAA
    GAGAAGAAGG AGGAGGAGGA AGACGACCGT GGGGAAGACG GCCCTAAGCC AATGCCTCCC
    TATAGCTCCA TGTTCATCCT GTCCACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATC CTCATGGTCA TTGCCATGAG CAGCATCGCC
    CTGGCCGCCG AGGACCCTGT GCAGCCCAAC GCACCTCGGA ACAACGTGCT GCGATACTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT TGACCTGGGG
    CTCGTCCTGC ATCAGGGTGC CTACTTCCGT GACCTCTGGA ATATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTAGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA AGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGCTG
    CCAAAGCTCA AGGCTGTGTT TGACTGTGTG GTGAACTCAC TTAAAAACGT CTTCAACATC
    CTCATCGTCT ACATGCTATT CATGTTCATC TTCGCCGTGG TGGCTGTGCA GCTCTTCAAG
    GGGAAATTCT TCCACTGCAC TGACGAGTCC AAAGAGTTTG AGAAAGATTG TCGAGGCAAA
    TACCTCCTCT ACGAGAAGAA TGAGGTGAAG GCGCGAGACC GGGAGTGGAA GAAGTATGAA
    TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCATTCGGTG GACGCCACCT TTGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATTTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ATATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AATACAGCCT GGAGAAAAAT GAGAGGGCCT GCATTGATTT CGCCATCAGC
    GCCAAGCCGC TGACCCGACA CATGCCGCAG AACAAGCAGA GCTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCTCCGCC TTTCGAGTAC ACGATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTTA TGATGAAGTT CTATGGGGCT TCTGTTGCTT ATGAAAATGC CCTGCGGGTG
    TTCAACATCG TCTTCACCTC CCTCTTCTCT CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTCCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ATATCCTCGT GACTGAGTTT GGGAATAACT TCATCAACCT GAGCTTTCTC
    CGCCTCTTCC GAGCTGCCCG GCTCATCAAA CTTCTCCGTC AGGGTTACAC CATCCGCATT
    CTTCTCTGGA CCTTTGTGCA GTCCTTCAAG GCCCTGCCTT ATGTCTGTCT GCTGATCGCC
    ATGCTCTTCT TCATCTATGC CATCATTGGG ATGCAGGTGT TTGGTAACAT TGGCATCGAC
    GTGGAGGACG AGGACAGTGA TGAAGATGAG TTCCAAATCA CTGAGCACAA TAACTTCCGG
    ACCTTCTTCC AGGCCCTCAT GCTTCTCTTC CGGAGTGCCA CCGGGGAAGC TTGGCACAAC
    ATCATGCTTT CCTGCCTCAG CGGGAAACCG TGTGATAAGA ACTCTGGCAT CCTGACTCGA
    GAGTGTGGCA ATGAATTTGC TTATTTTTAC TTTGTTTCCT TCATCTTCCT CTGCTCGTTT
    CTGATGCTGA ATCTCTTTGT CGCCGTCATC ATGGACAACT TTGAGTACCT CACCCGAGAC
    TCCTCCATCC TGGGCCCCCA CCACCTGGAT GAGTACGTGC GTGTCTGGGC CGAGTATGAC
    CCCGCAGCTT GCGGTCGGAT TCATTATAAG GATATGTACA GTTTATTACG AGTAATATCT
    CCCCCTCTCG GCTTAGGCAA GAAATGTCCT CATAGGGTTG CTTGCAAGCG GCTTCTGCGG
    ATGGACCTGC CCGTCGCAGA TGACAACACC GTCCACTTCA ATTCCACCCT CATGGCTCTG
    ATCCGCACAG CCCTGGACAT CAAGATTGCC AAGGGAGGAG CCGACAAACA GCAGATGGAC
    GCTGAGCTGC GGAAGGAGAT GATGGCGATT TGGCCCAATC TGTCCCAGAA GACGCTAGAC
    CTGCTGGTCA CACCTCACAA GTCCACGGAC CTCACCGTGG GGAAGATCTA CGCAGCCATG
    ATGATCATGG AGTACTACCG GCAGAGCAAG GCCAAGAAGC TGCAGGCCAT GCGCGAGGAG
    CAGGACCGGA CACCCCTCAT GTTCCAGCGC ATGGAGCCCC CGTCCCCAAC GCAGGAAGGG
    GGACCTGGCC AGAACGCCCT CCCCTCCACC CAGCTGGACC CAGGAGGAGC CCTGATGGCT
    CACGAAAGCG GCCTCAAGGA GAGCCCGTCC TGGGTGACCC AGCGTGCCCA GGAGATGTTC
    CAGAAGACGG GCACATGGAG TCCGGAACAA GGCCCCCCTA CCGACATGCC CAACAGCCAG
    CCTAACTCTC AGTCCGTGGA GATGCGAGAG ATGGGCAGAG ATGGCTACTC CGACAGCGAG
    CACTACCTCC CCATGGAAGG CCAGGGCCGG GCTGCCTCCA TGCCCCGCCT CCCTGCAGAG
    AACCAGAGGA GAAGGGGCCG GCCACGTGGG AATAACCTCA GTACCATCTC AGACACCAGC
    CCCATGAAGC GTTCAGCCTC CGTGCTGGGC CCCAAGGCCC GACGCCTGGA CGATTACTCG
    CTGGAGCGGG TCCCGCCCGA GGAGAACCAG CGGCACCACC AGCGGCGCCG CGACCGCAGC
    CACCGCGCCT CTGAGCGCTC CCTGGGCCGC TACACCGATG TGGACACAGG CTTGGGGACA
    GACCTGAGCA TGACCACCCA ATCCGGGGAC CTGCCGTCGA AGGAGCGGGA CCAGGAGCGG
    GGCCGGCCCA AGGATCGGAA GCATCGACAG CACCACCACC ACCACCACCA CCACCACCAT
    CCCCCGCCCC CCGACAAGGA CCGCTATGCC CAGGAACGGC CGGACCACGG CCGGGCACGG
    GCTCGGGACC AGCGCTGGTC CCGCTCGCCC AGCGAGGGCC GAGAGCACAT GGCGCACCGG
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.