PUS1 cDNA ORF clone, Homo sapiens(human)

The following PUS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03372 NM_001002020.3
Latest version!
Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu03372 NM_001002019.3
Latest version!
Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu32279 NM_025215.6
Latest version!
Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu72764 XM_011538768.3
Latest version!
Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu72765 XM_011538769.2
Latest version!
Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
View Old Accession Versions >>
OHu72764 XM_011538768.2 Homo sapiens pseudouridylate synthase 1 (PUS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu03372 NM_001002019.2 Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu32279 NM_025215.5 Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu03372 NM_001002020.2 Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu03372
    Clone ID Related Accession (Same CDS sequence) NM_001002019.3 , NM_001002020.2 , NM_001002019.2 , NM_001002020.3
    Accession Version NM_001002019.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX340929.2, BM923133.1, AF116238.1, AI566545.1 and AC131009.7. On May 31, 2019 this sequence version replaced NM_001002019.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.2045.1, SRR1163655.386344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002019.1
    CDS140..1339
    Translation

    Target ORF information:

    RefSeq Version NM_001002019.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002019.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03372
    Clone ID Related Accession (Same CDS sequence) NM_001002019.3 , NM_001002020.2 , NM_001002019.2 , NM_001002020.3
    Accession Version NM_001002020.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-13
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB118952.1, BC002901.2 and AI566545.1. This sequence is a reference standard in the RefSeqGene project. On Sep 24, 2009 this sequence version replaced NM_001002020.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.138333.1, BC002901.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002020.1
    CDS192..1391
    Translation

    Target ORF information:

    RefSeq Version NM_001002020.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002020.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03372
    Clone ID Related Accession (Same CDS sequence) NM_001002019.3 , NM_001002020.2 , NM_001002019.2 , NM_001002020.3
    Accession Version NM_001002019.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-03
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX340929.2, BM923133.1, AF116238.1 and AI566545.1. On Sep 24, 2009 this sequence version replaced NM_001002019.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.386344.1, SRR1163655.2045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002019.1
    CDS163..1362
    Translation

    Target ORF information:

    RefSeq Version NM_001002019.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002019.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03372
    Clone ID Related Accession (Same CDS sequence) NM_001002019.3 , NM_001002020.2 , NM_001002019.2 , NM_001002020.3
    Accession Version NM_001002020.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB118952.1, BC002901.2, AI566545.1 and AC131009.7. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001002020.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002901.2, SRR1163655.223644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002020.1
    CDS116..1315
    Translation

    Target ORF information:

    RefSeq Version NM_001002020.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002020.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCGGGA ACGCGGAGCC GCCGCCCGCC GGAGCCGCAT GCCCCCAGGA CCGGAGGTCC 
    TGCAGCGGCC GGGCCGGGGG CGACCGCGTC TGGGAGGACG GAGAACATCC GGCGAAGAAG
    CTCAAGAGCG GTGGCGACGA GGAGCGGCGC GAGAAGCCGC CCAAGCGGAA GATCGTGCTG
    CTCATGGCCT ATTCGGGCAA GGGCTACCAC GGCATGCAGA GGAATGTCGG GTCCTCACAA
    TTCAAAACAA TTGAAGATGA CTTGGTGTCC GCCCTCGTCC GGTCAGGCTG TATTCCTGAA
    AATCATGGTG AGGACATGAG GAAAATGTCC TTCCAGCGCT GCGCCCGGAC AGACAAGGGT
    GTGTCCGCAG CCGGCCAGGT GGTATCCCTG AAGGTGTGGC TGATTGACGA CATTCTAGAA
    AAGATCAACA GCCACCTTCC CTCTCACATT CGGATTCTGG GACTGAAGCG GGTCACGGGC
    GGGTTTAACT CCAAGAACAG ATGTGATGCC AGGACCTATT GCTACCTGCT GCCCACGTTT
    GCCTTTGCGC ACAAGGACCG GGACGTTCAG GATGAGACCT ACCGCCTGAG CGCCGAGACG
    CTGCAGCAGG TCAACAGGCT CCTGGCCTGC TACAAGGGCA CGCACAACTT CCACAATTTC
    ACCTCGCAGA AGGGGCCGCA GGATCCCAGT GCCTGCCGCT ACATCCTGGA GATGTACTGC
    GAGGAACCCT TTGTGCGGGA GGGCCTGGAG TTTGCGGTGA TCAGGGTGAA GGGCCAGAGC
    TTCATGATGC ATCAGATCCG GAAGATGGTC GGCCTGGTGG TGGCCATTGT GAAGGGTTAT
    GCCCCTGAGA GCGTGCTGGA GCGCAGCTGG GGCACAGAGA AGGTGGACGT GCCCAAGGCG
    CCCGGACTCG GCCTGGTCCT GGAGAGGGTG CACTTCGAGA AGTACAACCA GCGCTTTGGC
    AACGATGGGC TGCATGAGCC GCTGGACTGG GCGCAGGAGG AAGGAAAGGT CGCAGCCTTC
    AAGGAGGAGC ACATCTACCC CACCATCATC GGCACCGAGC GGGACGAACG CTCCATGGCC
    CAGTGGCTGA GCACCTTGCC CATCCACAAC TTCAGTGCCA CCGCTCTCAC GGCAGGTGGC
    ACGGGCGCCA AGGTGCCCAG TCCCCTGGAA GGCAGTGAAG GGGACGGAGA CACTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu32279
    Clone ID Related Accession (Same CDS sequence) NM_025215.5 , NM_025215.6
    Accession Version NM_025215.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK074659.1 and AI566545.1. On Sep 24, 2009 this sequence version replaced NM_025215.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074659.1, AF318369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCTCC AGCTTCGCGC GCTGTTGGGA GCCTTCGGAC GGTGGACCCT GCGCCTGGGA 
    CCGCGTCCGT CCTGCTCGCC GCGCATGGCC GGGAACGCGG AGCCGCCGCC CGCCGGAGCC
    GCATGCCCCC AGGACCGGAG GTCCTGCAGC GGCCGGGCCG GGGGCGACCG CGTCTGGGAG
    GACGGAGAAC ATCCGGCGAA GAAGCTCAAG AGCGGTGGCG ACGAGGAGCG GCGCGAGAAG
    CCGCCCAAGC GGAAGATCGT GCTGCTCATG GCCTATTCGG GCAAGGGCTA CCACGGCATG
    CAGAGGAATG TCGGGTCCTC ACAATTCAAA ACAATTGAAG ATGACTTGGT GTCCGCCCTC
    GTCCGGTCAG GCTGTATTCC TGAAAATCAT GGTGAGGACA TGAGGAAAAT GTCCTTCCAG
    CGCTGCGCCC GGACAGACAA GGGTGTGTCC GCAGCCGGCC AGGTGGTATC CCTGAAGGTG
    TGGCTGATTG ACGACATTCT AGAAAAGATC AACAGCCACC TTCCCTCTCA CATTCGGATT
    CTGGGACTGA AGCGGGTCAC GGGCGGGTTT AACTCCAAGA ACAGATGTGA TGCCAGGACC
    TATTGCTACC TGCTGCCCAC GTTTGCCTTT GCGCACAAGG ACCGGGACGT TCAGGATGAG
    ACCTACCGCC TGAGCGCCGA GACGCTGCAG CAGGTCAACA GGCTCCTGGC CTGCTACAAG
    GGCACGCACA ACTTCCACAA TTTCACCTCG CAGAAGGGGC CGCAGGATCC CAGTGCCTGC
    CGCTACATCC TGGAGATGTA CTGCGAGGAA CCCTTTGTGC GGGAGGGCCT GGAGTTTGCG
    GTGATCAGGG TGAAGGGCCA GAGCTTCATG ATGCATCAGA TCCGGAAGAT GGTCGGCCTG
    GTGGTGGCCA TTGTGAAGGG TTATGCCCCT GAGAGCGTGC TGGAGCGCAG CTGGGGCACA
    GAGAAGGTGG ACGTGCCCAA GGCGCCCGGA CTCGGCCTGG TCCTGGAGAG GGTGCACTTC
    GAGAAGTACA ACCAGCGCTT TGGCAACGAT GGGCTGCATG AGCCGCTGGA CTGGGCGCAG
    GAGGAAGGAA AGGTCGCAGC CTTCAAGGAG GAGCACATCT ACCCCACCAT CATCGGCACC
    GAGCGGGACG AACGCTCCAT GGCCCAGTGG CTGAGCACCT TGCCCATCCA CAACTTCAGT
    GCCACCGCTC TCACGGCAGG TGGCACGGGC GCCAAGGTGC CCAGTCCCCT GGAAGGCAGT
    GAAGGGGACG GAGACACTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079491.2
    CDS456..1739
    Translation

    Target ORF information:

    RefSeq Version NM_025215.5
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025215.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCTCC AGCTTCGCGC GCTGTTGGGA GCCTTCGGAC GGTGGACCCT GCGCCTGGGA 
    CCGCGTCCGT CCTGCTCGCC GCGCATGGCC GGGAACGCGG AGCCGCCGCC CGCCGGAGCC
    GCATGCCCCC AGGACCGGAG GTCCTGCAGC GGCCGGGCCG GGGGCGACCG CGTCTGGGAG
    GACGGAGAAC ATCCGGCGAA GAAGCTCAAG AGCGGTGGCG ACGAGGAGCG GCGCGAGAAG
    CCGCCCAAGC GGAAGATCGT GCTGCTCATG GCCTATTCGG GCAAGGGCTA CCACGGCATG
    CAGAGGAATG TCGGGTCCTC ACAATTCAAA ACAATTGAAG ATGACTTGGT GTCCGCCCTC
    GTCCGGTCAG GCTGTATTCC TGAAAATCAT GGTGAGGACA TGAGGAAAAT GTCCTTCCAG
    CGCTGCGCCC GGACAGACAA GGGTGTGTCC GCAGCCGGCC AGGTGGTATC CCTGAAGGTG
    TGGCTGATTG ACGACATTCT AGAAAAGATC AACAGCCACC TTCCCTCTCA CATTCGGATT
    CTGGGACTGA AGCGGGTCAC GGGCGGGTTT AACTCCAAGA ACAGATGTGA TGCCAGGACC
    TATTGCTACC TGCTGCCCAC GTTTGCCTTT GCGCACAAGG ACCGGGACGT TCAGGATGAG
    ACCTACCGCC TGAGCGCCGA GACGCTGCAG CAGGTCAACA GGCTCCTGGC CTGCTACAAG
    GGCACGCACA ACTTCCACAA TTTCACCTCG CAGAAGGGGC CGCAGGATCC CAGTGCCTGC
    CGCTACATCC TGGAGATGTA CTGCGAGGAA CCCTTTGTGC GGGAGGGCCT GGAGTTTGCG
    GTGATCAGGG TGAAGGGCCA GAGCTTCATG ATGCATCAGA TCCGGAAGAT GGTCGGCCTG
    GTGGTGGCCA TTGTGAAGGG TTATGCCCCT GAGAGCGTGC TGGAGCGCAG CTGGGGCACA
    GAGAAGGTGG ACGTGCCCAA GGCGCCCGGA CTCGGCCTGG TCCTGGAGAG GGTGCACTTC
    GAGAAGTACA ACCAGCGCTT TGGCAACGAT GGGCTGCATG AGCCGCTGGA CTGGGCGCAG
    GAGGAAGGAA AGGTCGCAGC CTTCAAGGAG GAGCACATCT ACCCCACCAT CATCGGCACC
    GAGCGGGACG AACGCTCCAT GGCCCAGTGG CTGAGCACCT TGCCCATCCA CAACTTCAGT
    GCCACCGCTC TCACGGCAGG TGGCACGGGC GCCAAGGTGC CCAGTCCCCT GGAAGGCAGT
    GAAGGGGACG GAGACACTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu32279
    Clone ID Related Accession (Same CDS sequence) NM_025215.5 , NM_025215.6
    Accession Version NM_025215.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK074659.1, AI566545.1 and AC131009.7. On May 17, 2019 this sequence version replaced NM_025215.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074659.1, AF318369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376649.8/ ENSP00000365837.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCTCC AGCTTCGCGC GCTGTTGGGA GCCTTCGGAC GGTGGACCCT GCGCCTGGGA 
    CCGCGTCCGT CCTGCTCGCC GCGCATGGCC GGGAACGCGG AGCCGCCGCC CGCCGGAGCC
    GCATGCCCCC AGGACCGGAG GTCCTGCAGC GGCCGGGCCG GGGGCGACCG CGTCTGGGAG
    GACGGAGAAC ATCCGGCGAA GAAGCTCAAG AGCGGTGGCG ACGAGGAGCG GCGCGAGAAG
    CCGCCCAAGC GGAAGATCGT GCTGCTCATG GCCTATTCGG GCAAGGGCTA CCACGGCATG
    CAGAGGAATG TCGGGTCCTC ACAATTCAAA ACAATTGAAG ATGACTTGGT GTCCGCCCTC
    GTCCGGTCAG GCTGTATTCC TGAAAATCAT GGTGAGGACA TGAGGAAAAT GTCCTTCCAG
    CGCTGCGCCC GGACAGACAA GGGTGTGTCC GCAGCCGGCC AGGTGGTATC CCTGAAGGTG
    TGGCTGATTG ACGACATTCT AGAAAAGATC AACAGCCACC TTCCCTCTCA CATTCGGATT
    CTGGGACTGA AGCGGGTCAC GGGCGGGTTT AACTCCAAGA ACAGATGTGA TGCCAGGACC
    TATTGCTACC TGCTGCCCAC GTTTGCCTTT GCGCACAAGG ACCGGGACGT TCAGGATGAG
    ACCTACCGCC TGAGCGCCGA GACGCTGCAG CAGGTCAACA GGCTCCTGGC CTGCTACAAG
    GGCACGCACA ACTTCCACAA TTTCACCTCG CAGAAGGGGC CGCAGGATCC CAGTGCCTGC
    CGCTACATCC TGGAGATGTA CTGCGAGGAA CCCTTTGTGC GGGAGGGCCT GGAGTTTGCG
    GTGATCAGGG TGAAGGGCCA GAGCTTCATG ATGCATCAGA TCCGGAAGAT GGTCGGCCTG
    GTGGTGGCCA TTGTGAAGGG TTATGCCCCT GAGAGCGTGC TGGAGCGCAG CTGGGGCACA
    GAGAAGGTGG ACGTGCCCAA GGCGCCCGGA CTCGGCCTGG TCCTGGAGAG GGTGCACTTC
    GAGAAGTACA ACCAGCGCTT TGGCAACGAT GGGCTGCATG AGCCGCTGGA CTGGGCGCAG
    GAGGAAGGAA AGGTCGCAGC CTTCAAGGAG GAGCACATCT ACCCCACCAT CATCGGCACC
    GAGCGGGACG AACGCTCCAT GGCCCAGTGG CTGAGCACCT TGCCCATCCA CAACTTCAGT
    GCCACCGCTC TCACGGCAGG TGGCACGGGC GCCAAGGTGC CCAGTCCCCT GGAAGGCAGT
    GAAGGGGACG GAGACACTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079491.2
    CDS448..1731
    Translation

    Target ORF information:

    RefSeq Version NM_025215.6
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025215.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCTCC AGCTTCGCGC GCTGTTGGGA GCCTTCGGAC GGTGGACCCT GCGCCTGGGA 
    CCGCGTCCGT CCTGCTCGCC GCGCATGGCC GGGAACGCGG AGCCGCCGCC CGCCGGAGCC
    GCATGCCCCC AGGACCGGAG GTCCTGCAGC GGCCGGGCCG GGGGCGACCG CGTCTGGGAG
    GACGGAGAAC ATCCGGCGAA GAAGCTCAAG AGCGGTGGCG ACGAGGAGCG GCGCGAGAAG
    CCGCCCAAGC GGAAGATCGT GCTGCTCATG GCCTATTCGG GCAAGGGCTA CCACGGCATG
    CAGAGGAATG TCGGGTCCTC ACAATTCAAA ACAATTGAAG ATGACTTGGT GTCCGCCCTC
    GTCCGGTCAG GCTGTATTCC TGAAAATCAT GGTGAGGACA TGAGGAAAAT GTCCTTCCAG
    CGCTGCGCCC GGACAGACAA GGGTGTGTCC GCAGCCGGCC AGGTGGTATC CCTGAAGGTG
    TGGCTGATTG ACGACATTCT AGAAAAGATC AACAGCCACC TTCCCTCTCA CATTCGGATT
    CTGGGACTGA AGCGGGTCAC GGGCGGGTTT AACTCCAAGA ACAGATGTGA TGCCAGGACC
    TATTGCTACC TGCTGCCCAC GTTTGCCTTT GCGCACAAGG ACCGGGACGT TCAGGATGAG
    ACCTACCGCC TGAGCGCCGA GACGCTGCAG CAGGTCAACA GGCTCCTGGC CTGCTACAAG
    GGCACGCACA ACTTCCACAA TTTCACCTCG CAGAAGGGGC CGCAGGATCC CAGTGCCTGC
    CGCTACATCC TGGAGATGTA CTGCGAGGAA CCCTTTGTGC GGGAGGGCCT GGAGTTTGCG
    GTGATCAGGG TGAAGGGCCA GAGCTTCATG ATGCATCAGA TCCGGAAGAT GGTCGGCCTG
    GTGGTGGCCA TTGTGAAGGG TTATGCCCCT GAGAGCGTGC TGGAGCGCAG CTGGGGCACA
    GAGAAGGTGG ACGTGCCCAA GGCGCCCGGA CTCGGCCTGG TCCTGGAGAG GGTGCACTTC
    GAGAAGTACA ACCAGCGCTT TGGCAACGAT GGGCTGCATG AGCCGCTGGA CTGGGCGCAG
    GAGGAAGGAA AGGTCGCAGC CTTCAAGGAG GAGCACATCT ACCCCACCAT CATCGGCACC
    GAGCGGGACG AACGCTCCAT GGCCCAGTGG CTGAGCACCT TGCCCATCCA CAACTTCAGT
    GCCACCGCTC TCACGGCAGG TGGCACGGGC GCCAAGGTGC CCAGTCCCCT GGAAGGCAGT
    GAAGGGGACG GAGACACTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72764
    Clone ID Related Accession (Same CDS sequence) XM_011538768.3 , XM_011538768.2
    Accession Version XM_011538768.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011538768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGGAAAA TGTCCTTCCA GCGCTGCGCC CGGACAGACA AGGGTGTGTC CGCAGCCGGC 
    CAGGTGGTAT CCCTGAAGGT GTGGCTGATT GACGACATTC TAGAAAAGAT CAACAGCCAC
    CTTCCCTCTC ACATTCGGAT TCTGGGACTG AAGCGGGTCA CGGGCGGGTT TAACTCCAAG
    AACAGATGTG ATGCCAGGAC CTATTGCTAC CTGCTGCCCA CGTTTGCCTT TGCGCACAAG
    GACCGGGACG TTCAGGATGA GACCTACCGC CTGAGCGCCG AGACGCTGCA GCAGGTCAAC
    AGGCTCCTGG CCTGCTACAA GGGCACGCAC AACTTCCACA ATTTCACCTC GCAGAAGGGG
    CCGCAGGATC CCAGTGCCTG CCGCTACATC CTGGAGATGT ACTGCGAGGA ACCCTTTGTG
    CGGGAGGGCC TGGAGTTTGC GGTGATCAGG GTGAAGGGCC AGAGCTTCAT GATGCATCAG
    ATCCGGAAGA TGGTCGGCCT GGTGGTGGCC ATTGTGAAGG GTTATGCCCC TGAGAGCGTG
    CTGGAGCGCA GCTGGGGCAC AGAGAAGGTG GACGTGCCCA AGGCGCCCGG ACTCGGCCTG
    GTCCTGGAGA GGGTGCACTT CGAGAAGTAC AACCAGCGCT TTGGCAACGA TGGGCTGCAT
    GAGCCGCTGG ACTGGGCGCA GGAGGAAGGA AAGGTCGCAG CCTTCAAGGA GGAGCACATC
    TACCCCACCA TCATCGGCAC CGAGCGGGAC GAACGCTCCA TGGCCCAGTG GCTGAGCACC
    TTGCCCATCC ACAACTTCAG TGCCACCGCT CTCACGGCAG GTGGCACGGG CGCCAAGGTG
    CCCAGTCCCC TGGAAGGCAG TGAAGGGGAC GGAGACACTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011537070.1
    CDS121..1005
    Translation

    Target ORF information:

    RefSeq Version XM_011538768.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538768.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGGAAAA TGTCCTTCCA GCGCTGCGCC CGGACAGACA AGGGTGTGTC CGCAGCCGGC 
    CAGGTGGTAT CCCTGAAGGT GTGGCTGATT GACGACATTC TAGAAAAGAT CAACAGCCAC
    CTTCCCTCTC ACATTCGGAT TCTGGGACTG AAGCGGGTCA CGGGCGGGTT TAACTCCAAG
    AACAGATGTG ATGCCAGGAC CTATTGCTAC CTGCTGCCCA CGTTTGCCTT TGCGCACAAG
    GACCGGGACG TTCAGGATGA GACCTACCGC CTGAGCGCCG AGACGCTGCA GCAGGTCAAC
    AGGCTCCTGG CCTGCTACAA GGGCACGCAC AACTTCCACA ATTTCACCTC GCAGAAGGGG
    CCGCAGGATC CCAGTGCCTG CCGCTACATC CTGGAGATGT ACTGCGAGGA ACCCTTTGTG
    CGGGAGGGCC TGGAGTTTGC GGTGATCAGG GTGAAGGGCC AGAGCTTCAT GATGCATCAG
    ATCCGGAAGA TGGTCGGCCT GGTGGTGGCC ATTGTGAAGG GTTATGCCCC TGAGAGCGTG
    CTGGAGCGCA GCTGGGGCAC AGAGAAGGTG GACGTGCCCA AGGCGCCCGG ACTCGGCCTG
    GTCCTGGAGA GGGTGCACTT CGAGAAGTAC AACCAGCGCT TTGGCAACGA TGGGCTGCAT
    GAGCCGCTGG ACTGGGCGCA GGAGGAAGGA AAGGTCGCAG CCTTCAAGGA GGAGCACATC
    TACCCCACCA TCATCGGCAC CGAGCGGGAC GAACGCTCCA TGGCCCAGTG GCTGAGCACC
    TTGCCCATCC ACAACTTCAG TGCCACCGCT CTCACGGCAG GTGGCACGGG CGCCAAGGTG
    CCCAGTCCCC TGGAAGGCAG TGAAGGGGAC GGAGACACTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72764
    Clone ID Related Accession (Same CDS sequence) XM_011538768.3 , XM_011538768.2
    Accession Version XM_011538768.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGGAAAA TGTCCTTCCA GCGCTGCGCC CGGACAGACA AGGGTGTGTC CGCAGCCGGC 
    CAGGTGGTAT CCCTGAAGGT GTGGCTGATT GACGACATTC TAGAAAAGAT CAACAGCCAC
    CTTCCCTCTC ACATTCGGAT TCTGGGACTG AAGCGGGTCA CGGGCGGGTT TAACTCCAAG
    AACAGATGTG ATGCCAGGAC CTATTGCTAC CTGCTGCCCA CGTTTGCCTT TGCGCACAAG
    GACCGGGACG TTCAGGATGA GACCTACCGC CTGAGCGCCG AGACGCTGCA GCAGGTCAAC
    AGGCTCCTGG CCTGCTACAA GGGCACGCAC AACTTCCACA ATTTCACCTC GCAGAAGGGG
    CCGCAGGATC CCAGTGCCTG CCGCTACATC CTGGAGATGT ACTGCGAGGA ACCCTTTGTG
    CGGGAGGGCC TGGAGTTTGC GGTGATCAGG GTGAAGGGCC AGAGCTTCAT GATGCATCAG
    ATCCGGAAGA TGGTCGGCCT GGTGGTGGCC ATTGTGAAGG GTTATGCCCC TGAGAGCGTG
    CTGGAGCGCA GCTGGGGCAC AGAGAAGGTG GACGTGCCCA AGGCGCCCGG ACTCGGCCTG
    GTCCTGGAGA GGGTGCACTT CGAGAAGTAC AACCAGCGCT TTGGCAACGA TGGGCTGCAT
    GAGCCGCTGG ACTGGGCGCA GGAGGAAGGA AAGGTCGCAG CCTTCAAGGA GGAGCACATC
    TACCCCACCA TCATCGGCAC CGAGCGGGAC GAACGCTCCA TGGCCCAGTG GCTGAGCACC
    TTGCCCATCC ACAACTTCAG TGCCACCGCT CTCACGGCAG GTGGCACGGG CGCCAAGGTG
    CCCAGTCCCC TGGAAGGCAG TGAAGGGGAC GGAGACACTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011537070.1
    CDS120..1004
    Translation

    Target ORF information:

    RefSeq Version XM_011538768.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridylate synthase 1 (PUS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538768.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGGAAAA TGTCCTTCCA GCGCTGCGCC CGGACAGACA AGGGTGTGTC CGCAGCCGGC 
    CAGGTGGTAT CCCTGAAGGT GTGGCTGATT GACGACATTC TAGAAAAGAT CAACAGCCAC
    CTTCCCTCTC ACATTCGGAT TCTGGGACTG AAGCGGGTCA CGGGCGGGTT TAACTCCAAG
    AACAGATGTG ATGCCAGGAC CTATTGCTAC CTGCTGCCCA CGTTTGCCTT TGCGCACAAG
    GACCGGGACG TTCAGGATGA GACCTACCGC CTGAGCGCCG AGACGCTGCA GCAGGTCAAC
    AGGCTCCTGG CCTGCTACAA GGGCACGCAC AACTTCCACA ATTTCACCTC GCAGAAGGGG
    CCGCAGGATC CCAGTGCCTG CCGCTACATC CTGGAGATGT ACTGCGAGGA ACCCTTTGTG
    CGGGAGGGCC TGGAGTTTGC GGTGATCAGG GTGAAGGGCC AGAGCTTCAT GATGCATCAG
    ATCCGGAAGA TGGTCGGCCT GGTGGTGGCC ATTGTGAAGG GTTATGCCCC TGAGAGCGTG
    CTGGAGCGCA GCTGGGGCAC AGAGAAGGTG GACGTGCCCA AGGCGCCCGG ACTCGGCCTG
    GTCCTGGAGA GGGTGCACTT CGAGAAGTAC AACCAGCGCT TTGGCAACGA TGGGCTGCAT
    GAGCCGCTGG ACTGGGCGCA GGAGGAAGGA AAGGTCGCAG CCTTCAAGGA GGAGCACATC
    TACCCCACCA TCATCGGCAC CGAGCGGGAC GAACGCTCCA TGGCCCAGTG GCTGAGCACC
    TTGCCCATCC ACAACTTCAG TGCCACCGCT CTCACGGCAG GTGGCACGGG CGCCAAGGTG
    CCCAGTCCCC TGGAAGGCAG TGAAGGGGAC GGAGACACTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72765
    Clone ID Related Accession (Same CDS sequence) XM_011538769.2
    Accession Version XM_011538769.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tRNA pseudouridine synthase A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGCCTCC AGCTTCGCGC GCTGTTGGGA GCCTTCGGAC GGTGGACCCT GCGCCTGGGA 
    CCGCGTCCGT CCTGCTCGCC GCGCATGGCC GGGAACGCGG AGCCGCCGCC CGCCGGAGCC
    GCATGCCCCC AGGACCGGAG GTCCTGCAGC GGCCGGGCCG GGGGCGACCG CGTCTGGGAG
    GACGGAGAAC ATCCGGCGAA GAAGCTCAAG AGCGGTGGCG ACGAGGAGCG GCGCGAGAAG
    CCGCCCAAGC GGAAGATCGT GCTGCTCATG GCCTATTCGG GCAAGGGCTA CCACGGCATG
    CAGAGGAATG TCGGGTCCTC ACAATTCAAA ACAATTGAAG ATGACTTGGT GTCCGCCCTC
    GTCCGGTCAG GCTGTATTCC TGAAAATCAT GGTGAGGACA TGAGGAAAAT GTCCTTCCAG
    CGCTGCGCCC GGACAGACAA GAGTCAGGAT CTCATTCTGT TGCCCAGGCT GGAGTGCAGT
    GGCATGATCA TGGCTCACTG CAGTCTCAAA CTGCTGGGCT CAAGCAATCC TCCTGCCTCA
    GCCTCCCGAG TAGCTCGTGC CTTCAGTCTC TTGCCTCGGC ACCTGGGTGG CTTACCGCCC
    ACAACCCAAC GTTTGGCAGA CGGGGACAGC AGAGCCGGAG AGAACAGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011537071.1
    CDS456..1106
    Translation

    Target ORF information:

    RefSeq Version XM_011538769.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pseudouridine synthase 1 (PUS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGCCTCC AGCTTCGCGC GCTGTTGGGA GCCTTCGGAC GGTGGACCCT GCGCCTGGGA 
    CCGCGTCCGT CCTGCTCGCC GCGCATGGCC GGGAACGCGG AGCCGCCGCC CGCCGGAGCC
    GCATGCCCCC AGGACCGGAG GTCCTGCAGC GGCCGGGCCG GGGGCGACCG CGTCTGGGAG
    GACGGAGAAC ATCCGGCGAA GAAGCTCAAG AGCGGTGGCG ACGAGGAGCG GCGCGAGAAG
    CCGCCCAAGC GGAAGATCGT GCTGCTCATG GCCTATTCGG GCAAGGGCTA CCACGGCATG
    CAGAGGAATG TCGGGTCCTC ACAATTCAAA ACAATTGAAG ATGACTTGGT GTCCGCCCTC
    GTCCGGTCAG GCTGTATTCC TGAAAATCAT GGTGAGGACA TGAGGAAAAT GTCCTTCCAG
    CGCTGCGCCC GGACAGACAA GAGTCAGGAT CTCATTCTGT TGCCCAGGCT GGAGTGCAGT
    GGCATGATCA TGGCTCACTG CAGTCTCAAA CTGCTGGGCT CAAGCAATCC TCCTGCCTCA
    GCCTCCCGAG TAGCTCGTGC CTTCAGTCTC TTGCCTCGGC ACCTGGGTGG CTTACCGCCC
    ACAACCCAAC GTTTGGCAGA CGGGGACAGC AGAGCCGGAG AGAACAGATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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