×

RNASEH2C cDNA ORF clone, Homo sapiens(Human)

Gene Symbol RNASEH2C
Entrez Gene ID 84153
Full Name ribonuclease H2 subunit C
Synonyms AGS3,AYP1
General protein information
Preferred Names

ribonuclease H2 subunit C

Names

ribonuclease H2 subunit C
RNase H1 small subunit
RNase H2 subunit C
aicardi-Goutieres syndrome 3 protein
ribonuclease HI subunit C

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q13.1

Summary This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

610330

Disorder Html: Aicardi-Goutieres syndrome 3, 610329 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_032193.3 NP_115569.2 ribonuclease H2 subunit C



Canis lupus familiaris (dog) RNASEH2C XP_852826.1
Rattus norvegicus (Norway rat) Rnaseh2c XP_002728902.1
Macaca mulatta (rhesus monkey) LOC701279 XP_001089023.1
Pan troglodytes (chimpanzee) RNASEH2C XP_508559.3
Mus musculus (house mouse) Rnaseh2c NP_080892.1
Macaca mulatta (rhesus monkey) RNASEH2C XP_001085695.1
Homo sapiens (human) RNASEH2C NP_115569.2
Bos taurus (cattle) RNASEH2C NP_001039386.1


Related articles in PubMed

Altered spatio-temporal dynamics of RNase H2 complex assembly at replication and repair sites in Aicardi-Goutières syndrome.
Kind B, Muster B, Staroske W, Herce HD, Sachse R, Rapp A, Schmidt F, Koss S, Cardoso MC, Lee-Kirsch MA
Human molecular genetics23(22)5950-60(2014 Nov)

The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects.
Figiel M, Chon H, Cerritelli SM, Cybulska M, Crouch RJ, Nowotny M
The Journal of biological chemistry286(12)10540-50(2011 Mar)

New loci associated with kidney function and chronic kidney disease.
Köttgen A, Pattaro C, Böger CA, Fuchsberger C, Olden M, Glazer NL, Parsa A, Gao X, Yang Q, Smith AV, O'Connell JR, Li M, Schmidt H, Tanaka T, Isaacs A, Ketkar S, Hwang SJ, Johnson AD, Dehghan A, Teumer A, Paré G, Atkinson EJ, Zeller T, Lohman K, Cornelis MC, Probst-Hensch NM, Kronenberg F, Tönjes A, Hayward C, Aspelund T, Eiriksdottir G, Launer LJ, Harris TB, Rampersaud E, Mitchell BD, Arking DE, Boerwinkle E, Struchalin M, Cavalieri M, Singleton A, Giallauria F, Metter J, de Boer IH, Haritunians T, Lumley T, Siscovick D, Psaty BM, Zillikens MC, Oostra BA, Feitosa M, Province M, de Andrade M, Turner ST, Schillert A, Ziegler A, Wild PS, Schnabel RB, Wilde S, Munzel TF, Leak TS, Illig T, Klopp N, Meisinger C, Wichmann HE, Koenig W, Zgaga L, Zemunik T, Kolcic I, Minelli C, Hu FB, Johansson A, Igl W, Zaboli G, Wild SH, Wright AF, Campbell H, Ellinghaus D, Schreiber S, Aulchenko YS, Felix JF, Rivadeneira F, Uitterlinden AG, Hofman A, Imboden M, Nitsch D, Brandstätter A, Kollerits B, Kedenko L, Mägi R, Stumvoll M, Kovacs P, Boban M, Campbell S, Endlich K, Völzke H, Kroemer HK, Nauck M, Völker U, Polasek O, Vitart V, Badola S, Parker AN, Ridker PM, Kardia SL, Blankenberg S, Liu Y, Curhan GC, Franke A, Rochat T, Paulweber B, Prokopenko I, Wang W, Gudnason V, Shuldiner AR, Coresh J, Schmidt R, Ferrucci L, Shlipak MG, van Duijn CM, Borecki I, Krämer BK, Rudan I, Gyllensten U, Wilson JF, Witteman JC, Pramstaller PP, Rettig R, Hastie N, Chasman DI, Kao WH, Heid IM, Fox CS
Nature genetics42(5)376-84(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reviewed that Neurologic Phenotypes Associated with Mutations in RNASEH2C in patients with Aicardi-Goutieres Syndrome.
Title: Neurologic Phenotypes Associated with Mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, ADAR1, and IFIH1: Aicardi-Goutières Syndrome and Beyond.

RNase H2 complex is assembled in the cytosol and imported into the nucleus in an RNase H2B-dependent manner.
Title: Altered spatio-temporal dynamics of RNase H2 complex assembly at replication and repair sites in Aicardi-Goutières syndrome.

Examination of the effect of several Aicardi-Goutieres Syndrome-related mutations in the C subunit of RNase H2.
Title: Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex.

Describes an RNASEH2C pseudogene that is located close to the SRY gene on chromosome Y.
Title: Evidence that the SRY protein is encoded by a single exon on the human Y chromosome.

The following RNASEH2C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNASEH2C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

Free DYK Tag Antibody

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20612
NM_032193.3
Latest version!
Homo sapiens ribonuclease H2 subunit C (RNASEH2C), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20612
Clone ID Related Accession (Same CDS sequence) NM_032193.3
Accession Version NM_032193.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-28
Organism Homo sapiens(Human)
Product ribonuclease H2 subunit C
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF346606.1, BC023588.2, AP001266.4 and BC000831.1. This sequence is a reference standard in the RefSeqGene project. On Aug 25, 2006 this sequence version replaced NM_032193.2. ##Evidence-Data-START## Transcript exon combination :: AF346606.1, BX353248.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGAGAGCG GCGACGAAGC GGCCATCGAG AGGCACCGCG TCCACTTGCG CTCCGCCACA 
TTGCGCGACG CCGTACCCGC CACACTGCAT CTGCTGCCCT GCGAGGTTGC GGTGGACGGG
CCCGCCCCGG TGGGGCGCTT CTTCACGCCC GCCATCCGCC AGGGCCCCGA GGGACTCGAA
GTGTCGTTTC GGGGCCGCTG TCTACGGGGA GAGGAGGTGG CGGTGCCGCC TGGCCTCGTG
GGATACGTGA TGGTGACAGA AGAGAAGAAG GTGTCGATGG GGAAGCCAGA CCCCTTGCGG
GATTCCGGGA CTGACGACCA AGAGGAGGAG CCGCTGGAGC GGGACTTCGA CCGCTTCATT
GGAGCCACTG CCAACTTCAG CCGCTTCACC CTGTGGGGTC TGGAGACCAT CCCTGGCCCG
GATGCCAAAG TGCGTGGGGC CTTAACTTGG CCCAGCCTTG CGGCAGCGAT TCACGCACAG
GTGCCCGAGG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_115569.2
CDS181..675
Misc Feature(1)64..66
Misc Feature(2)181..183
Misc Feature(3)187..189
Misc Feature(4)262..651
Misc Feature(5)order(268..282,337..339,358..360,364..381,421..432)
Exon (1)1..352
Exon (2)353..528
Exon (3)529..648
Exon (4)649..2785
Translation

Target ORF information:

RefSeq Version NM_032193.3
Organism Homo sapiens(Human)
Definition Homo sapiens ribonuclease H2 subunit C (RNASEH2C), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032193.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGAGAGCG GCGACGAAGC GGCCATCGAG AGGCACCGCG TCCACTTGCG CTCCGCCACA 
TTGCGCGACG CCGTACCCGC CACACTGCAT CTGCTGCCCT GCGAGGTTGC GGTGGACGGG
CCCGCCCCGG TGGGGCGCTT CTTCACGCCC GCCATCCGCC AGGGCCCCGA GGGACTCGAA
GTGTCGTTTC GGGGCCGCTG TCTACGGGGA GAGGAGGTGG CGGTGCCGCC TGGCCTCGTG
GGATACGTGA TGGTGACAGA AGAGAAGAAG GTGTCGATGG GGAAGCCAGA CCCCTTGCGG
GATTCCGGGA CTGACGACCA AGAGGAGGAG CCGCTGGAGC GGGACTTCGA CCGCTTCATT
GGAGCCACTG CCAACTTCAG CCGCTTCACC CTGTGGGGTC TGGAGACCAT CCCTGGCCCG
GATGCCAAAG TGCGTGGGGC CTTAACTTGG CCCAGCCTTG CGGCAGCGAT TCACGCACAG
GTGCCCGAGG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Altered spatio-temporal dynamics of RNase H2 complex assembly at replication and repair sites in Aicardi-Goutières syndrome.
Human molecular genetics23(22)5950-60(2014 Nov)
Kind B,Muster B,Staroske W,Herce HD,Sachse R,Rapp A,Schmidt F,Koss S,Cardoso MC,Lee-Kirsch MA


book

The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects.
The Journal of biological chemistry286(12)10540-50(2011 Mar)
Figiel M,Chon H,Cerritelli SM,Cybulska M,Crouch RJ,Nowotny M


book

New loci associated with kidney function and chronic kidney disease.
Nature genetics42(5)376-84(2010 May)
Köttgen A,Pattaro C,Böger CA,Fuchsberger C,Olden M,Glazer NL,Parsa A,Gao X,Yang Q,Smith AV,O'Connell JR,Li M,Schmidt H,Tanaka T,Isaacs A,Ketkar S,Hwang SJ,Johnson AD,Dehghan A,Teumer A,Paré G,Atkinson EJ,Zeller T,Lohman K,Cornelis MC,Probst-Hensch NM,Kronenberg F,Tönjes A,Hayward C,Aspelund T,Eiriksdottir G,Launer LJ,Harris TB,Rampersaud E,Mitchell BD,Arking DE,Boerwinkle E,Struchalin M,Cavalieri M,Singleton A,Giallauria F,Metter J,de Boer IH,Haritunians T,Lumley T,Siscovick D,Psaty BM,Zillikens MC,Oostra BA,Feitosa M,Province M,de Andrade M,Turner ST,Schillert A,Ziegler A,Wild PS,Schnabel RB,Wilde S,Munzel TF,Leak TS,Illig T,Klopp N,Meisinger C,Wichmann HE,Koenig W,Zgaga L,Zemunik T,Kolcic I,Minelli C,Hu FB,Johansson A,Igl W,Zaboli G,Wild SH,Wright AF,Campbell H,Ellinghaus D,Schreiber S,Aulchenko YS,Felix JF,Rivadeneira F,Uitterlinden AG,Hofman A,Imboden M,Nitsch D,Brandstätter A,Kollerits B,Kedenko L,Mägi R,Stumvoll M,Kovacs P,Boban M,Campbell S,Endlich K,Völzke H,Kroemer HK,Nauck M,Völker U,Polasek O,Vitart V,Badola S,Parker AN,Ridker PM,Kardia SL,Blankenberg S,Liu Y,Curhan GC,Franke A,Rochat T,Paulweber B,Prokopenko I,Wang W,Gudnason V,Shuldiner AR,Coresh J,Schmidt R,Ferrucci L,Shlipak MG,van Duijn CM,Borecki I,Krämer BK,Rudan I,Gyllensten U,Wilson JF,Witteman JC,Pramstaller PP,Rettig R,Hastie N,Chasman DI,Kao WH,Heid IM,Fox CS


 
*
*
*
*