MAP3K6 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following MAP3K6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu90784 XM_017002771.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu28867 NM_004672.5
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $909.30
$1299.00
OHu28868 NM_001297609.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu90785 XM_017002772.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu28867 NM_004672.4 Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $909.30
$1299.00
OHu28868 NM_001297609.1 Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu90784
    Clone ID Related Accession (Same CDS sequence) XM_017002771.1
    Accession Version XM_017002771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGCTGG GCTGCCTGCT GGCCCGCAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
    GATGTGGGTT TCTACCTGGG AGCCCAGATC CTCGCCAATG ACCCCACCCA GGTGGTGCTG
    GCTGCAGAGC AGCTGTATAA GCTCAATGCC CCCATATGGT ACCTGGTGTC CGTGATGGAG
    ACCTTCCTGC TCTACCAGCA CTTCAGGCCC ACGCCAGAGC CCCCTGGAGG GCCACCACGC
    CGTGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AACCATTCAA GACAGCCTGT
    GCCCAGGGCG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ACAAGGTGCT GCTGCCTGCA
    AAGCTCGAGG TTCGGGGTAC TGACCCAGTA AGCACAGTGA CCCTGAGCCT GCTGGAGCCT
    GAGACCCAGG ACATTCCCTC CAGCTGGACC TTCCCAGTCG CCTCCATATG CGGAGTCAGC
    GCCTCAAAGC GCGACGAGCG CTGCTGCTTC CTCTATGCAC TCCCCCCGGC TCAGGACGTC
    CAGCTGTGCT TCCCCAGCGT AGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTGG
    GTGACGAACC CGGATTCCAC GGCGCCCGCG GAGGAGGCGG AGGGCGCGGG GGAGATGTTG
    GAGTTTGATT ATGAGTACAC GGAGACGGGC GAGCGGCTGG TGCTGGGCAA GGGCACGTAT
    GGGGTGGTGT ACGCGGGCCG CGATCGCCAC ACGAGGGTGC GCATCGCCAT CAAGGAGATC
    CCGGAGCGGG ACAGCAGGTT CTCTCAGCCC CTGCATGAAG AGATCGCTCT TCACAGACGC
    CTGCGCCACA AGAACATAGT GCGCTATCTG GGCTCAGCTA GCCAGGGCGG CTACCTTAAG
    ATCTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCCTCCT TGCTGCGGTC GGTGTGGGGA
    CCCCTGAAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTT
    GGCTACTTGC ACGACAACCA CATCGTGCAC AGGGACATAA AAGGGGACAA TGTGCTGATC
    AACACCTTCA GTGGGCTGCT CAAGATTTCT GACTTCGGCA CCTCCAAGCG GCTGGCAGGC
    ATCACACCTT GCACTGAGAC CTTCACAGGA ACTCTGCAGT ATATGGCCCC AGAAATCATT
    GACCAGGGCC CACGCGGGTA TGGGAAAGCA GCTGACATCT GGTCACTGGG CTGCACTGTC
    ATTGAGATGG CCACAGGTCG CCCCCCCTTC CACGAGCTCG GGAGCCCACA GGCTGCCATG
    TTTCAGGTGG GTATGTACAA GGTCCATCCG CCAATGCCCA GCTCTCTGTC GGCCGAGGCC
    CAAGCCTTTC TCCTCCGAAC TTTTGAGCCA GACCCCCGCC TCCGAGCCAG CGCCCAGACA
    CTGCTGGGGG ACCCCTTCCT GCAGCCTGGG AAAAGGAGCC GCAGCCCCAG CTCCCCACGA
    CATGCTCCAC GGCCCTCAGA TGCCCCTTCT GCCAGTCCCA CTCCTTCAGC CAACTCAACC
    ACCCAGTCTC AGACATTCCC GTGCCCTCAG GCACCCTCTC AGCACCCACC CAGCCCCCCG
    AAGCGCTGCC TCAGTTATGG GGGCACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGCGGCC
    GAGGAGCCTG CGTCTCCGGA GGAGAGTTCG GGGCTGAGCC TGCTGCACCA GGAGAGCAAG
    CGTCGGGCCA TGCTGGCCGC AGTATTGGAG CAGGAGCTGC CAGCGCTGGC GGAGAATCTG
    CACCAGGAGC AGAAGCAAGA GCAGGGGGCC CGTCTGGGCA GAAACCATGT GGAAGAGCTG
    CTGCGCTGCC TCGGGGCACA CATCCACACT CCCAACCGCC GGCAGCTCGC CCAGGAGCTG
    CGGGCGCTGC AAGGACGGCT GAGGGCCCAG GGCCTTGGGC CTGCGCTTCT GCACAGACCG
    CTGTTTGCCT TCCCGGATGC GGTGAAGCAG ATCCTCCGCA AGCGCCAGAT CCGTCCACAC
    TGGATGTTCG TTCTGGACTC ACTGCTCAGC CGTGCTGTGC GGGCAGCCCT GGGTGTGCTA
    GGACCGGAGG TGGAGAAGGA GGCGGTCTCA CCGAGGTCAG AGGAGCTGAG TAATGAAGGG
    GACTCCCAGC AGAGCCCAGG CCAGCAGAGC CCGCTTCCGG TGGAGCCCGA GCAGGGCCCC
    GCTCCTCTGA TGGTGCAGCT GAGCCTCTTG AGGGCAGAGA CTGATCGGCT GCGCGAAATC
    CTGGCGGGGA AGGAACGGGA GTACCAGGCC CTGGTGCAGC GGGCTCTACA GCGGCTGAAT
    GAGGAAGCCC GGACCTATGT CCTGGCCCCA GAGCCTCCAA CTGCTCTTTC AACGGACCAG
    GGCCTGGTGC AGTGGCTACA GGAACTGAAT GTGGATTCAG GCACCATCCA AATGCTGTTG
    AACCATAGCT TCACCCTCCA CACTCTGCTC ACCTATGCCA CTCGAGATGA CCTCATCTAC
    ACCCGCATCA GGGGAGGGAT GGTATGCCGC ATCTGGAGGG CCATCTTGGC ACAGCGAGCA
    GGATCCACAC CAGTCACCTC TGGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858260.1
    CDS908..3517
    Translation

    Target ORF information:

    RefSeq Version XM_017002771.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGCTGG GCTGCCTGCT GGCCCGCAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG 
    GATGTGGGTT TCTACCTGGG AGCCCAGATC CTCGCCAATG ACCCCACCCA GGTGGTGCTG
    GCTGCAGAGC AGCTGTATAA GCTCAATGCC CCCATATGGT ACCTGGTGTC CGTGATGGAG
    ACCTTCCTGC TCTACCAGCA CTTCAGGCCC ACGCCAGAGC CCCCTGGAGG GCCACCACGC
    CGTGCCCACT TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AACCATTCAA GACAGCCTGT
    GCCCAGGGCG ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ACAAGGTGCT GCTGCCTGCA
    AAGCTCGAGG TTCGGGGTAC TGACCCAGTA AGCACAGTGA CCCTGAGCCT GCTGGAGCCT
    GAGACCCAGG ACATTCCCTC CAGCTGGACC TTCCCAGTCG CCTCCATATG CGGAGTCAGC
    GCCTCAAAGC GCGACGAGCG CTGCTGCTTC CTCTATGCAC TCCCCCCGGC TCAGGACGTC
    CAGCTGTGCT TCCCCAGCGT AGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTGG
    GTGACGAACC CGGATTCCAC GGCGCCCGCG GAGGAGGCGG AGGGCGCGGG GGAGATGTTG
    GAGTTTGATT ATGAGTACAC GGAGACGGGC GAGCGGCTGG TGCTGGGCAA GGGCACGTAT
    GGGGTGGTGT ACGCGGGCCG CGATCGCCAC ACGAGGGTGC GCATCGCCAT CAAGGAGATC
    CCGGAGCGGG ACAGCAGGTT CTCTCAGCCC CTGCATGAAG AGATCGCTCT TCACAGACGC
    CTGCGCCACA AGAACATAGT GCGCTATCTG GGCTCAGCTA GCCAGGGCGG CTACCTTAAG
    ATCTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCCTCCT TGCTGCGGTC GGTGTGGGGA
    CCCCTGAAGG ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTT
    GGCTACTTGC ACGACAACCA CATCGTGCAC AGGGACATAA AAGGGGACAA TGTGCTGATC
    AACACCTTCA GTGGGCTGCT CAAGATTTCT GACTTCGGCA CCTCCAAGCG GCTGGCAGGC
    ATCACACCTT GCACTGAGAC CTTCACAGGA ACTCTGCAGT ATATGGCCCC AGAAATCATT
    GACCAGGGCC CACGCGGGTA TGGGAAAGCA GCTGACATCT GGTCACTGGG CTGCACTGTC
    ATTGAGATGG CCACAGGTCG CCCCCCCTTC CACGAGCTCG GGAGCCCACA GGCTGCCATG
    TTTCAGGTGG GTATGTACAA GGTCCATCCG CCAATGCCCA GCTCTCTGTC GGCCGAGGCC
    CAAGCCTTTC TCCTCCGAAC TTTTGAGCCA GACCCCCGCC TCCGAGCCAG CGCCCAGACA
    CTGCTGGGGG ACCCCTTCCT GCAGCCTGGG AAAAGGAGCC GCAGCCCCAG CTCCCCACGA
    CATGCTCCAC GGCCCTCAGA TGCCCCTTCT GCCAGTCCCA CTCCTTCAGC CAACTCAACC
    ACCCAGTCTC AGACATTCCC GTGCCCTCAG GCACCCTCTC AGCACCCACC CAGCCCCCCG
    AAGCGCTGCC TCAGTTATGG GGGCACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGCGGCC
    GAGGAGCCTG CGTCTCCGGA GGAGAGTTCG GGGCTGAGCC TGCTGCACCA GGAGAGCAAG
    CGTCGGGCCA TGCTGGCCGC AGTATTGGAG CAGGAGCTGC CAGCGCTGGC GGAGAATCTG
    CACCAGGAGC AGAAGCAAGA GCAGGGGGCC CGTCTGGGCA GAAACCATGT GGAAGAGCTG
    CTGCGCTGCC TCGGGGCACA CATCCACACT CCCAACCGCC GGCAGCTCGC CCAGGAGCTG
    CGGGCGCTGC AAGGACGGCT GAGGGCCCAG GGCCTTGGGC CTGCGCTTCT GCACAGACCG
    CTGTTTGCCT TCCCGGATGC GGTGAAGCAG ATCCTCCGCA AGCGCCAGAT CCGTCCACAC
    TGGATGTTCG TTCTGGACTC ACTGCTCAGC CGTGCTGTGC GGGCAGCCCT GGGTGTGCTA
    GGACCGGAGG TGGAGAAGGA GGCGGTCTCA CCGAGGTCAG AGGAGCTGAG TAATGAAGGG
    GACTCCCAGC AGAGCCCAGG CCAGCAGAGC CCGCTTCCGG TGGAGCCCGA GCAGGGCCCC
    GCTCCTCTGA TGGTGCAGCT GAGCCTCTTG AGGGCAGAGA CTGATCGGCT GCGCGAAATC
    CTGGCGGGGA AGGAACGGGA GTACCAGGCC CTGGTGCAGC GGGCTCTACA GCGGCTGAAT
    GAGGAAGCCC GGACCTATGT CCTGGCCCCA GAGCCTCCAA CTGCTCTTTC AACGGACCAG
    GGCCTGGTGC AGTGGCTACA GGAACTGAAT GTGGATTCAG GCACCATCCA AATGCTGTTG
    AACCATAGCT TCACCCTCCA CACTCTGCTC ACCTATGCCA CTCGAGATGA CCTCATCTAC
    ACCCGCATCA GGGGAGGGAT GGTATGCCGC ATCTGGAGGG CCATCTTGGC ACAGCGAGCA
    GGATCCACAC CAGTCACCTC TGGACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28867
    Clone ID Related Accession (Same CDS sequence) NM_004672.4 , NM_004672.5
    Accession Version NM_004672.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 6 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW771625.1, AB167411.1, AF100318.1 and AI658745.1. This sequence is a reference standard in the RefSeqGene project. On Jul 4, 2014 this sequence version replaced NM_004672.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB167411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GAGGATGTTT TCCAGAAGAA CTCGGATTGC GTTGGCAGCT ACACACTGAT CCCCTATGTG
    GTGACGGCCA CTGGTCGGGT GCTGTGTGGT GATGCAGGCC TTCTGCGGGG CCTGGCTGAT
    GGGCTGGTAC AGGCTGGAGT GGGGACCGAG GCCCTGCTCA CTCCCCTGGT GGGCCGGCTT
    GCCCGCCTGC TGGAGGCCAC ACCCACAGAC TCTTGTGGCT ATTTCCGGGA GACCATTCGG
    CGGGACATCC GGCAGGCGCG GGAGCGGTTC AGTGGGCCAC AGCTGCGGCA GGAGCTGGCT
    CGCCTGCAGC GGAGACTGGA CAGCGTGGAG CTGCTGAGCC CCGACATCAT CATGAACTTG
    CTGCTCTCCT ACCGCGATGT GCAGGACTAC TCGGCCATCA TTGAGCTGGT GGAGACGCTG
    CAGGCCTTGC CCACCTGTGA TGTGGCCGAG CAGCATAATG TCTGCTTCCA CTACACTTTT
    GCCCTCAACC GGAGGAACAG GCCTGGGGAC CGGGCGAAGG CCCTGTCTGT GCTGCTGCCG
    CTGGTACAGC TTGAGGGCTC TGTGGCGCCC GATCTGTACT GCATGTGTGG CCGTATCTAC
    AAGGACATGT TCTTCAGCTC GGGTTTCCAG GATGCTGGGC ACCGGGAGCA GGCCTATCAC
    TGGTATCGCA AGGCTTTTGA CGTAGAGCCC AGCCTTCACT CAGGCATCAA TGCAGCTGTG
    CTCCTCATTG CTGCCGGGCA GCACTTTGAG GATTCCAAAG AGCTCCGGCT AATAGGCATG
    AAGCTGGGCT GCCTGCTGGC CCGCAAAGGC TGCGTGGAGA AGATGCAGTA TTACTGGGAT
    GTGGGTTTCT ACCTGGGAGC CCAGATCCTC GCCAATGACC CCACCCAGGT GGTGCTGGCT
    GCAGAGCAGC TGTATAAGCT CAATGCCCCC ATATGGTACC TGGTGTCCGT GATGGAGACC
    TTCCTGCTCT ACCAGCACTT CAGGCCCACG CCAGAGCCCC CTGGAGGGCC ACCACGCCGT
    GCCCACTTCT GGCTCCACTT CTTGCTACAG TCCTGCCAAC CATTCAAGAC AGCCTGTGCC
    CAGGGCGACC AGTGCTTGGT GCTGGTCCTG GAGATGAACA AGGTGCTGCT GCCTGCAAAG
    CTCGAGGTTC GGGGTACTGA CCCAGTAAGC ACAGTGACCC TGAGCCTGCT GGAGCCTGAG
    ACCCAGGACA TTCCCTCCAG CTGGACCTTC CCAGTCGCCT CCATATGCGG AGTCAGCGCC
    TCAAAGCGCG ACGAGCGCTG CTGCTTCCTC TATGCACTCC CCCCGGCTCA GGACGTCCAG
    CTGTGCTTCC CCAGCGTAGG GCACTGCCAG TGGTTCTGCG GCCTGATCCA GGCCTGGGTG
    ACGAACCCGG ATTCCACGGC GCCCGCGGAG GAGGCGGAGG GCGCGGGGGA GATGTTGGAG
    TTTGATTATG AGTACACGGA GACGGGCGAG CGGCTGGTGC TGGGCAAGGG CACGTATGGG
    GTGGTGTACG CGGGCCGCGA TCGCCACACG AGGGTGCGCA TCGCCATCAA GGAGATCCCG
    GAGCGGGACA GCAGGTTCTC TCAGCCCCTG CATGAAGAGA TCGCTCTTCA CAGACGCCTG
    CGCCACAAGA ACATAGTGCG CTATCTGGGC TCAGCTAGCC AGGGCGGCTA CCTTAAGATC
    TTCATGGAGG AAGTGCCTGG AGGCAGCCTG TCCTCCTTGC TGCGGTCGGT GTGGGGACCC
    CTGAAGGACA ACGAGAGCAC CATCAGTTTC TACACCCGCC AGATCCTGCA GGGACTTGGC
    TACTTGCACG ACAACCACAT CGTGCACAGG GACATAAAAG GGGACAATGT GCTGATCAAC
    ACCTTCAGTG GGCTGCTCAA GATTTCTGAC TTCGGCACCT CCAAGCGGCT GGCAGGCATC
    ACACCTTGCA CTGAGACCTT CACAGGAACT CTGCAGTATA TGGCCCCAGA AATCATTGAC
    CAGGGCCCAC GCGGGTATGG GAAAGCAGCT GACATCTGGT CACTGGGCTG CACTGTCATT
    GAGATGGCCA CAGGTCGCCC CCCCTTCCAC GAGCTCGGGA GCCCACAGGC TGCCATGTTT
    CAGGTGGGTA TGTACAAGGT CCATCCGCCA ATGCCCAGCT CTCTGTCGGC CGAGGCCCAA
    GCCTTTCTCC TCCGAACTTT TGAGCCAGAC CCCCGCCTCC GAGCCAGCGC CCAGACACTG
    CTGGGGGACC CCTTCCTGCA GCCTGGGAAA AGGAGCCGCA GCCCCAGCTC CCCACGACAT
    GCTCCACGGC CCTCAGATGC CCCTTCTGCC AGTCCCACTC CTTCAGCCAA CTCAACCACC
    CAGTCTCAGA CATTCCCGTG CCCTCAGGCA CCCTCTCAGC ACCCACCCAG CCCCCCGAAG
    CGCTGCCTCA GTTATGGGGG CACCAGCCAG CTCCGGGTGC CCGAGGAGCC TGCGGCCGAG
    GAGCCTGCGT CTCCGGAGGA GAGTTCGGGG CTGAGCCTGC TGCACCAGGA GAGCAAGCGT
    CGGGCCATGC TGGCCGCAGT ATTGGAGCAG GAGCTGCCAG CGCTGGCGGA GAATCTGCAC
    CAGGAGCAGA AGCAAGAGCA GGGGGCCCGT CTGGGCAGAA ACCATGTGGA AGAGCTGCTG
    CGCTGCCTCG GGGCACACAT CCACACTCCC AACCGCCGGC AGCTCGCCCA GGAGCTGCGG
    GCGCTGCAAG GACGGCTGAG GGCCCAGGGC CTTGGGCCTG CGCTTCTGCA CAGACCGCTG
    TTTGCCTTCC CGGATGCGGT GAAGCAGATC CTCCGCAAGC GCCAGATCCG TCCACACTGG
    ATGTTCGTTC TGGACTCACT GCTCAGCCGT GCTGTGCGGG CAGCCCTGGG TGTGCTAGGA
    CCGGAGGTGG AGAAGGAGGC GGTCTCACCG AGGTCAGAGG AGCTGAGTAA TGAAGGGGAC
    TCCCAGCAGA GCCCAGGCCA GCAGAGCCCG CTTCCGGTGG AGCCCGAGCA GGGCCCCGCT
    CCTCTGATGG TGCAGCTGAG CCTCTTGAGG GCAGAGACTG ATCGGCTGCG CGAAATCCTG
    GCGGGGAAGG AACGGGAGTA CCAGGCCCTG GTGCAGCGGG CTCTACAGCG GCTGAATGAG
    GAAGCCCGGA CCTATGTCCT GGCCCCAGAG CCTCCAACTG CTCTTTCAAC GGACCAGGGC
    CTGGTGCAGT GGCTACAGGA ACTGAATGTG GATTCAGGCA CCATCCAAAT GCTGTTGAAC
    CATAGCTTCA CCCTCCACAC TCTGCTCACC TATGCCACTC GAGATGACCT CATCTACACC
    CGCATCAGGG GAGGGATGGT ATGCCGCATC TGGAGGGCCA TCTTGGCACA GCGAGCAGGA
    TCCACACCAG TCACCTCTGG ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004663.3
    CDS270..4136
    Translation

    Target ORF information:

    RefSeq Version NM_004672.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004672.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GAGGATGTTT TCCAGAAGAA CTCGGATTGC GTTGGCAGCT ACACACTGAT CCCCTATGTG
    GTGACGGCCA CTGGTCGGGT GCTGTGTGGT GATGCAGGCC TTCTGCGGGG CCTGGCTGAT
    GGGCTGGTAC AGGCTGGAGT GGGGACCGAG GCCCTGCTCA CTCCCCTGGT GGGCCGGCTT
    GCCCGCCTGC TGGAGGCCAC ACCCACAGAC TCTTGTGGCT ATTTCCGGGA GACCATTCGG
    CGGGACATCC GGCAGGCGCG GGAGCGGTTC AGTGGGCCAC AGCTGCGGCA GGAGCTGGCT
    CGCCTGCAGC GGAGACTGGA CAGCGTGGAG CTGCTGAGCC CCGACATCAT CATGAACTTG
    CTGCTCTCCT ACCGCGATGT GCAGGACTAC TCGGCCATCA TTGAGCTGGT GGAGACGCTG
    CAGGCCTTGC CCACCTGTGA TGTGGCCGAG CAGCATAATG TCTGCTTCCA CTACACTTTT
    GCCCTCAACC GGAGGAACAG GCCTGGGGAC CGGGCGAAGG CCCTGTCTGT GCTGCTGCCG
    CTGGTACAGC TTGAGGGCTC TGTGGCGCCC GATCTGTACT GCATGTGTGG CCGTATCTAC
    AAGGACATGT TCTTCAGCTC GGGTTTCCAG GATGCTGGGC ACCGGGAGCA GGCCTATCAC
    TGGTATCGCA AGGCTTTTGA CGTAGAGCCC AGCCTTCACT CAGGCATCAA TGCAGCTGTG
    CTCCTCATTG CTGCCGGGCA GCACTTTGAG GATTCCAAAG AGCTCCGGCT AATAGGCATG
    AAGCTGGGCT GCCTGCTGGC CCGCAAAGGC TGCGTGGAGA AGATGCAGTA TTACTGGGAT
    GTGGGTTTCT ACCTGGGAGC CCAGATCCTC GCCAATGACC CCACCCAGGT GGTGCTGGCT
    GCAGAGCAGC TGTATAAGCT CAATGCCCCC ATATGGTACC TGGTGTCCGT GATGGAGACC
    TTCCTGCTCT ACCAGCACTT CAGGCCCACG CCAGAGCCCC CTGGAGGGCC ACCACGCCGT
    GCCCACTTCT GGCTCCACTT CTTGCTACAG TCCTGCCAAC CATTCAAGAC AGCCTGTGCC
    CAGGGCGACC AGTGCTTGGT GCTGGTCCTG GAGATGAACA AGGTGCTGCT GCCTGCAAAG
    CTCGAGGTTC GGGGTACTGA CCCAGTAAGC ACAGTGACCC TGAGCCTGCT GGAGCCTGAG
    ACCCAGGACA TTCCCTCCAG CTGGACCTTC CCAGTCGCCT CCATATGCGG AGTCAGCGCC
    TCAAAGCGCG ACGAGCGCTG CTGCTTCCTC TATGCACTCC CCCCGGCTCA GGACGTCCAG
    CTGTGCTTCC CCAGCGTAGG GCACTGCCAG TGGTTCTGCG GCCTGATCCA GGCCTGGGTG
    ACGAACCCGG ATTCCACGGC GCCCGCGGAG GAGGCGGAGG GCGCGGGGGA GATGTTGGAG
    TTTGATTATG AGTACACGGA GACGGGCGAG CGGCTGGTGC TGGGCAAGGG CACGTATGGG
    GTGGTGTACG CGGGCCGCGA TCGCCACACG AGGGTGCGCA TCGCCATCAA GGAGATCCCG
    GAGCGGGACA GCAGGTTCTC TCAGCCCCTG CATGAAGAGA TCGCTCTTCA CAGACGCCTG
    CGCCACAAGA ACATAGTGCG CTATCTGGGC TCAGCTAGCC AGGGCGGCTA CCTTAAGATC
    TTCATGGAGG AAGTGCCTGG AGGCAGCCTG TCCTCCTTGC TGCGGTCGGT GTGGGGACCC
    CTGAAGGACA ACGAGAGCAC CATCAGTTTC TACACCCGCC AGATCCTGCA GGGACTTGGC
    TACTTGCACG ACAACCACAT CGTGCACAGG GACATAAAAG GGGACAATGT GCTGATCAAC
    ACCTTCAGTG GGCTGCTCAA GATTTCTGAC TTCGGCACCT CCAAGCGGCT GGCAGGCATC
    ACACCTTGCA CTGAGACCTT CACAGGAACT CTGCAGTATA TGGCCCCAGA AATCATTGAC
    CAGGGCCCAC GCGGGTATGG GAAAGCAGCT GACATCTGGT CACTGGGCTG CACTGTCATT
    GAGATGGCCA CAGGTCGCCC CCCCTTCCAC GAGCTCGGGA GCCCACAGGC TGCCATGTTT
    CAGGTGGGTA TGTACAAGGT CCATCCGCCA ATGCCCAGCT CTCTGTCGGC CGAGGCCCAA
    GCCTTTCTCC TCCGAACTTT TGAGCCAGAC CCCCGCCTCC GAGCCAGCGC CCAGACACTG
    CTGGGGGACC CCTTCCTGCA GCCTGGGAAA AGGAGCCGCA GCCCCAGCTC CCCACGACAT
    GCTCCACGGC CCTCAGATGC CCCTTCTGCC AGTCCCACTC CTTCAGCCAA CTCAACCACC
    CAGTCTCAGA CATTCCCGTG CCCTCAGGCA CCCTCTCAGC ACCCACCCAG CCCCCCGAAG
    CGCTGCCTCA GTTATGGGGG CACCAGCCAG CTCCGGGTGC CCGAGGAGCC TGCGGCCGAG
    GAGCCTGCGT CTCCGGAGGA GAGTTCGGGG CTGAGCCTGC TGCACCAGGA GAGCAAGCGT
    CGGGCCATGC TGGCCGCAGT ATTGGAGCAG GAGCTGCCAG CGCTGGCGGA GAATCTGCAC
    CAGGAGCAGA AGCAAGAGCA GGGGGCCCGT CTGGGCAGAA ACCATGTGGA AGAGCTGCTG
    CGCTGCCTCG GGGCACACAT CCACACTCCC AACCGCCGGC AGCTCGCCCA GGAGCTGCGG
    GCGCTGCAAG GACGGCTGAG GGCCCAGGGC CTTGGGCCTG CGCTTCTGCA CAGACCGCTG
    TTTGCCTTCC CGGATGCGGT GAAGCAGATC CTCCGCAAGC GCCAGATCCG TCCACACTGG
    ATGTTCGTTC TGGACTCACT GCTCAGCCGT GCTGTGCGGG CAGCCCTGGG TGTGCTAGGA
    CCGGAGGTGG AGAAGGAGGC GGTCTCACCG AGGTCAGAGG AGCTGAGTAA TGAAGGGGAC
    TCCCAGCAGA GCCCAGGCCA GCAGAGCCCG CTTCCGGTGG AGCCCGAGCA GGGCCCCGCT
    CCTCTGATGG TGCAGCTGAG CCTCTTGAGG GCAGAGACTG ATCGGCTGCG CGAAATCCTG
    GCGGGGAAGG AACGGGAGTA CCAGGCCCTG GTGCAGCGGG CTCTACAGCG GCTGAATGAG
    GAAGCCCGGA CCTATGTCCT GGCCCCAGAG CCTCCAACTG CTCTTTCAAC GGACCAGGGC
    CTGGTGCAGT GGCTACAGGA ACTGAATGTG GATTCAGGCA CCATCCAAAT GCTGTTGAAC
    CATAGCTTCA CCCTCCACAC TCTGCTCACC TATGCCACTC GAGATGACCT CATCTACACC
    CGCATCAGGG GAGGGATGGT ATGCCGCATC TGGAGGGCCA TCTTGGCACA GCGAGCAGGA
    TCCACACCAG TCACCTCTGG ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28867
    Clone ID Related Accession (Same CDS sequence) NM_004672.4 , NM_004672.5
    Accession Version NM_004672.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 6 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FO393419.1, AW771625.1, AB167411.1 and AF100318.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004672.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB167411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357582.3/ ENSP00000350195.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GAGGATGTTT TCCAGAAGAA CTCGGATTGC GTTGGCAGCT ACACACTGAT CCCCTATGTG
    GTGACGGCCA CTGGTCGGGT GCTGTGTGGT GATGCAGGCC TTCTGCGGGG CCTGGCTGAT
    GGGCTGGTAC AGGCTGGAGT GGGGACCGAG GCCCTGCTCA CTCCCCTGGT GGGCCGGCTT
    GCCCGCCTGC TGGAGGCCAC ACCCACAGAC TCTTGTGGCT ATTTCCGGGA GACCATTCGG
    CGGGACATCC GGCAGGCGCG GGAGCGGTTC AGTGGGCCAC AGCTGCGGCA GGAGCTGGCT
    CGCCTGCAGC GGAGACTGGA CAGCGTGGAG CTGCTGAGCC CCGACATCAT CATGAACTTG
    CTGCTCTCCT ACCGCGATGT GCAGGACTAC TCGGCCATCA TTGAGCTGGT GGAGACGCTG
    CAGGCCTTGC CCACCTGTGA TGTGGCCGAG CAGCATAATG TCTGCTTCCA CTACACTTTT
    GCCCTCAACC GGAGGAACAG GCCTGGGGAC CGGGCGAAGG CCCTGTCTGT GCTGCTGCCG
    CTGGTACAGC TTGAGGGCTC TGTGGCGCCC GATCTGTACT GCATGTGTGG CCGTATCTAC
    AAGGACATGT TCTTCAGCTC GGGTTTCCAG GATGCTGGGC ACCGGGAGCA GGCCTATCAC
    TGGTATCGCA AGGCTTTTGA CGTAGAGCCC AGCCTTCACT CAGGCATCAA TGCAGCTGTG
    CTCCTCATTG CTGCCGGGCA GCACTTTGAG GATTCCAAAG AGCTCCGGCT AATAGGCATG
    AAGCTGGGCT GCCTGCTGGC CCGCAAAGGC TGCGTGGAGA AGATGCAGTA TTACTGGGAT
    GTGGGTTTCT ACCTGGGAGC CCAGATCCTC GCCAATGACC CCACCCAGGT GGTGCTGGCT
    GCAGAGCAGC TGTATAAGCT CAATGCCCCC ATATGGTACC TGGTGTCCGT GATGGAGACC
    TTCCTGCTCT ACCAGCACTT CAGGCCCACG CCAGAGCCCC CTGGAGGGCC ACCACGCCGT
    GCCCACTTCT GGCTCCACTT CTTGCTACAG TCCTGCCAAC CATTCAAGAC AGCCTGTGCC
    CAGGGCGACC AGTGCTTGGT GCTGGTCCTG GAGATGAACA AGGTGCTGCT GCCTGCAAAG
    CTCGAGGTTC GGGGTACTGA CCCAGTAAGC ACAGTGACCC TGAGCCTGCT GGAGCCTGAG
    ACCCAGGACA TTCCCTCCAG CTGGACCTTC CCAGTCGCCT CCATATGCGG AGTCAGCGCC
    TCAAAGCGCG ACGAGCGCTG CTGCTTCCTC TATGCACTCC CCCCGGCTCA GGACGTCCAG
    CTGTGCTTCC CCAGCGTAGG GCACTGCCAG TGGTTCTGCG GCCTGATCCA GGCCTGGGTG
    ACGAACCCGG ATTCCACGGC GCCCGCGGAG GAGGCGGAGG GCGCGGGGGA GATGTTGGAG
    TTTGATTATG AGTACACGGA GACGGGCGAG CGGCTGGTGC TGGGCAAGGG CACGTATGGG
    GTGGTGTACG CGGGCCGCGA TCGCCACACG AGGGTGCGCA TCGCCATCAA GGAGATCCCG
    GAGCGGGACA GCAGGTTCTC TCAGCCCCTG CATGAAGAGA TCGCTCTTCA CAGACGCCTG
    CGCCACAAGA ACATAGTGCG CTATCTGGGC TCAGCTAGCC AGGGCGGCTA CCTTAAGATC
    TTCATGGAGG AAGTGCCTGG AGGCAGCCTG TCCTCCTTGC TGCGGTCGGT GTGGGGACCC
    CTGAAGGACA ACGAGAGCAC CATCAGTTTC TACACCCGCC AGATCCTGCA GGGACTTGGC
    TACTTGCACG ACAACCACAT CGTGCACAGG GACATAAAAG GGGACAATGT GCTGATCAAC
    ACCTTCAGTG GGCTGCTCAA GATTTCTGAC TTCGGCACCT CCAAGCGGCT GGCAGGCATC
    ACACCTTGCA CTGAGACCTT CACAGGAACT CTGCAGTATA TGGCCCCAGA AATCATTGAC
    CAGGGCCCAC GCGGGTATGG GAAAGCAGCT GACATCTGGT CACTGGGCTG CACTGTCATT
    GAGATGGCCA CAGGTCGCCC CCCCTTCCAC GAGCTCGGGA GCCCACAGGC TGCCATGTTT
    CAGGTGGGTA TGTACAAGGT CCATCCGCCA ATGCCCAGCT CTCTGTCGGC CGAGGCCCAA
    GCCTTTCTCC TCCGAACTTT TGAGCCAGAC CCCCGCCTCC GAGCCAGCGC CCAGACACTG
    CTGGGGGACC CCTTCCTGCA GCCTGGGAAA AGGAGCCGCA GCCCCAGCTC CCCACGACAT
    GCTCCACGGC CCTCAGATGC CCCTTCTGCC AGTCCCACTC CTTCAGCCAA CTCAACCACC
    CAGTCTCAGA CATTCCCGTG CCCTCAGGCA CCCTCTCAGC ACCCACCCAG CCCCCCGAAG
    CGCTGCCTCA GTTATGGGGG CACCAGCCAG CTCCGGGTGC CCGAGGAGCC TGCGGCCGAG
    GAGCCTGCGT CTCCGGAGGA GAGTTCGGGG CTGAGCCTGC TGCACCAGGA GAGCAAGCGT
    CGGGCCATGC TGGCCGCAGT ATTGGAGCAG GAGCTGCCAG CGCTGGCGGA GAATCTGCAC
    CAGGAGCAGA AGCAAGAGCA GGGGGCCCGT CTGGGCAGAA ACCATGTGGA AGAGCTGCTG
    CGCTGCCTCG GGGCACACAT CCACACTCCC AACCGCCGGC AGCTCGCCCA GGAGCTGCGG
    GCGCTGCAAG GACGGCTGAG GGCCCAGGGC CTTGGGCCTG CGCTTCTGCA CAGACCGCTG
    TTTGCCTTCC CGGATGCGGT GAAGCAGATC CTCCGCAAGC GCCAGATCCG TCCACACTGG
    ATGTTCGTTC TGGACTCACT GCTCAGCCGT GCTGTGCGGG CAGCCCTGGG TGTGCTAGGA
    CCGGAGGTGG AGAAGGAGGC GGTCTCACCG AGGTCAGAGG AGCTGAGTAA TGAAGGGGAC
    TCCCAGCAGA GCCCAGGCCA GCAGAGCCCG CTTCCGGTGG AGCCCGAGCA GGGCCCCGCT
    CCTCTGATGG TGCAGCTGAG CCTCTTGAGG GCAGAGACTG ATCGGCTGCG CGAAATCCTG
    GCGGGGAAGG AACGGGAGTA CCAGGCCCTG GTGCAGCGGG CTCTACAGCG GCTGAATGAG
    GAAGCCCGGA CCTATGTCCT GGCCCCAGAG CCTCCAACTG CTCTTTCAAC GGACCAGGGC
    CTGGTGCAGT GGCTACAGGA ACTGAATGTG GATTCAGGCA CCATCCAAAT GCTGTTGAAC
    CATAGCTTCA CCCTCCACAC TCTGCTCACC TATGCCACTC GAGATGACCT CATCTACACC
    CGCATCAGGG GAGGGATGGT ATGCCGCATC TGGAGGGCCA TCTTGGCACA GCGAGCAGGA
    TCCACACCAG TCACCTCTGG ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004663.3
    CDS365..4231
    Translation

    Target ORF information:

    RefSeq Version NM_004672.5
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004672.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GAGGATGTTT TCCAGAAGAA CTCGGATTGC GTTGGCAGCT ACACACTGAT CCCCTATGTG
    GTGACGGCCA CTGGTCGGGT GCTGTGTGGT GATGCAGGCC TTCTGCGGGG CCTGGCTGAT
    GGGCTGGTAC AGGCTGGAGT GGGGACCGAG GCCCTGCTCA CTCCCCTGGT GGGCCGGCTT
    GCCCGCCTGC TGGAGGCCAC ACCCACAGAC TCTTGTGGCT ATTTCCGGGA GACCATTCGG
    CGGGACATCC GGCAGGCGCG GGAGCGGTTC AGTGGGCCAC AGCTGCGGCA GGAGCTGGCT
    CGCCTGCAGC GGAGACTGGA CAGCGTGGAG CTGCTGAGCC CCGACATCAT CATGAACTTG
    CTGCTCTCCT ACCGCGATGT GCAGGACTAC TCGGCCATCA TTGAGCTGGT GGAGACGCTG
    CAGGCCTTGC CCACCTGTGA TGTGGCCGAG CAGCATAATG TCTGCTTCCA CTACACTTTT
    GCCCTCAACC GGAGGAACAG GCCTGGGGAC CGGGCGAAGG CCCTGTCTGT GCTGCTGCCG
    CTGGTACAGC TTGAGGGCTC TGTGGCGCCC GATCTGTACT GCATGTGTGG CCGTATCTAC
    AAGGACATGT TCTTCAGCTC GGGTTTCCAG GATGCTGGGC ACCGGGAGCA GGCCTATCAC
    TGGTATCGCA AGGCTTTTGA CGTAGAGCCC AGCCTTCACT CAGGCATCAA TGCAGCTGTG
    CTCCTCATTG CTGCCGGGCA GCACTTTGAG GATTCCAAAG AGCTCCGGCT AATAGGCATG
    AAGCTGGGCT GCCTGCTGGC CCGCAAAGGC TGCGTGGAGA AGATGCAGTA TTACTGGGAT
    GTGGGTTTCT ACCTGGGAGC CCAGATCCTC GCCAATGACC CCACCCAGGT GGTGCTGGCT
    GCAGAGCAGC TGTATAAGCT CAATGCCCCC ATATGGTACC TGGTGTCCGT GATGGAGACC
    TTCCTGCTCT ACCAGCACTT CAGGCCCACG CCAGAGCCCC CTGGAGGGCC ACCACGCCGT
    GCCCACTTCT GGCTCCACTT CTTGCTACAG TCCTGCCAAC CATTCAAGAC AGCCTGTGCC
    CAGGGCGACC AGTGCTTGGT GCTGGTCCTG GAGATGAACA AGGTGCTGCT GCCTGCAAAG
    CTCGAGGTTC GGGGTACTGA CCCAGTAAGC ACAGTGACCC TGAGCCTGCT GGAGCCTGAG
    ACCCAGGACA TTCCCTCCAG CTGGACCTTC CCAGTCGCCT CCATATGCGG AGTCAGCGCC
    TCAAAGCGCG ACGAGCGCTG CTGCTTCCTC TATGCACTCC CCCCGGCTCA GGACGTCCAG
    CTGTGCTTCC CCAGCGTAGG GCACTGCCAG TGGTTCTGCG GCCTGATCCA GGCCTGGGTG
    ACGAACCCGG ATTCCACGGC GCCCGCGGAG GAGGCGGAGG GCGCGGGGGA GATGTTGGAG
    TTTGATTATG AGTACACGGA GACGGGCGAG CGGCTGGTGC TGGGCAAGGG CACGTATGGG
    GTGGTGTACG CGGGCCGCGA TCGCCACACG AGGGTGCGCA TCGCCATCAA GGAGATCCCG
    GAGCGGGACA GCAGGTTCTC TCAGCCCCTG CATGAAGAGA TCGCTCTTCA CAGACGCCTG
    CGCCACAAGA ACATAGTGCG CTATCTGGGC TCAGCTAGCC AGGGCGGCTA CCTTAAGATC
    TTCATGGAGG AAGTGCCTGG AGGCAGCCTG TCCTCCTTGC TGCGGTCGGT GTGGGGACCC
    CTGAAGGACA ACGAGAGCAC CATCAGTTTC TACACCCGCC AGATCCTGCA GGGACTTGGC
    TACTTGCACG ACAACCACAT CGTGCACAGG GACATAAAAG GGGACAATGT GCTGATCAAC
    ACCTTCAGTG GGCTGCTCAA GATTTCTGAC TTCGGCACCT CCAAGCGGCT GGCAGGCATC
    ACACCTTGCA CTGAGACCTT CACAGGAACT CTGCAGTATA TGGCCCCAGA AATCATTGAC
    CAGGGCCCAC GCGGGTATGG GAAAGCAGCT GACATCTGGT CACTGGGCTG CACTGTCATT
    GAGATGGCCA CAGGTCGCCC CCCCTTCCAC GAGCTCGGGA GCCCACAGGC TGCCATGTTT
    CAGGTGGGTA TGTACAAGGT CCATCCGCCA ATGCCCAGCT CTCTGTCGGC CGAGGCCCAA
    GCCTTTCTCC TCCGAACTTT TGAGCCAGAC CCCCGCCTCC GAGCCAGCGC CCAGACACTG
    CTGGGGGACC CCTTCCTGCA GCCTGGGAAA AGGAGCCGCA GCCCCAGCTC CCCACGACAT
    GCTCCACGGC CCTCAGATGC CCCTTCTGCC AGTCCCACTC CTTCAGCCAA CTCAACCACC
    CAGTCTCAGA CATTCCCGTG CCCTCAGGCA CCCTCTCAGC ACCCACCCAG CCCCCCGAAG
    CGCTGCCTCA GTTATGGGGG CACCAGCCAG CTCCGGGTGC CCGAGGAGCC TGCGGCCGAG
    GAGCCTGCGT CTCCGGAGGA GAGTTCGGGG CTGAGCCTGC TGCACCAGGA GAGCAAGCGT
    CGGGCCATGC TGGCCGCAGT ATTGGAGCAG GAGCTGCCAG CGCTGGCGGA GAATCTGCAC
    CAGGAGCAGA AGCAAGAGCA GGGGGCCCGT CTGGGCAGAA ACCATGTGGA AGAGCTGCTG
    CGCTGCCTCG GGGCACACAT CCACACTCCC AACCGCCGGC AGCTCGCCCA GGAGCTGCGG
    GCGCTGCAAG GACGGCTGAG GGCCCAGGGC CTTGGGCCTG CGCTTCTGCA CAGACCGCTG
    TTTGCCTTCC CGGATGCGGT GAAGCAGATC CTCCGCAAGC GCCAGATCCG TCCACACTGG
    ATGTTCGTTC TGGACTCACT GCTCAGCCGT GCTGTGCGGG CAGCCCTGGG TGTGCTAGGA
    CCGGAGGTGG AGAAGGAGGC GGTCTCACCG AGGTCAGAGG AGCTGAGTAA TGAAGGGGAC
    TCCCAGCAGA GCCCAGGCCA GCAGAGCCCG CTTCCGGTGG AGCCCGAGCA GGGCCCCGCT
    CCTCTGATGG TGCAGCTGAG CCTCTTGAGG GCAGAGACTG ATCGGCTGCG CGAAATCCTG
    GCGGGGAAGG AACGGGAGTA CCAGGCCCTG GTGCAGCGGG CTCTACAGCG GCTGAATGAG
    GAAGCCCGGA CCTATGTCCT GGCCCCAGAG CCTCCAACTG CTCTTTCAAC GGACCAGGGC
    CTGGTGCAGT GGCTACAGGA ACTGAATGTG GATTCAGGCA CCATCCAAAT GCTGTTGAAC
    CATAGCTTCA CCCTCCACAC TCTGCTCACC TATGCCACTC GAGATGACCT CATCTACACC
    CGCATCAGGG GAGGGATGGT ATGCCGCATC TGGAGGGCCA TCTTGGCACA GCGAGCAGGA
    TCCACACCAG TCACCTCTGG ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28868
    Clone ID Related Accession (Same CDS sequence) NM_001297609.2 , NM_001297609.1
    Accession Version NM_001297609.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 6 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FO393419.1, AW771625.1, AB208805.1 and AI658745.1. On May 31, 2019 this sequence version replaced NM_001297609.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB208805.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GATTGCGTTG GCAGCTACAC ACTGATCCCC TATGTGGTGA CGGCCACTGG TCGGGTGCTG
    TGTGGTGATG CAGGCCTTCT GCGGGGCCTG GCTGATGGGC TGGTACAGGC TGGAGTGGGG
    ACCGAGGCCC TGCTCACTCC CCTGGTGGGC CGGCTTGCCC GCCTGCTGGA GGCCACACCC
    ACAGACTCTT GTGGCTATTT CCGGGAGACC ATTCGGCGGG ACATCCGGCA GGCGCGGGAG
    CGGTTCAGTG GGCCACAGCT GCGGCAGGAG CTGGCTCGCC TGCAGCGGAG ACTGGACAGC
    GTGGAGCTGC TGAGCCCCGA CATCATCATG AACTTGCTGC TCTCCTACCG CGATGTGCAG
    GACTACTCGG CCATCATTGA GCTGGTGGAG ACGCTGCAGG CCTTGCCCAC CTGTGATGTG
    GCCGAGCAGC ATAATGTCTG CTTCCACTAC ACTTTTGCCC TCAACCGGAG GAACAGGCCT
    GGGGACCGGG CGAAGGCCCT GTCTGTGCTG CTGCCGCTGG TACAGCTTGA GGGCTCTGTG
    GCGCCCGATC TGTACTGCAT GTGTGGCCGT ATCTACAAGG ACATGTTCTT CAGCTCGGGT
    TTCCAGGATG CTGGGCACCG GGAGCAGGCC TATCACTGGT ATCGCAAGGC TTTTGACGTA
    GAGCCCAGCC TTCACTCAGG CATCAATGCA GCTGTGCTCC TCATTGCTGC CGGGCAGCAC
    TTTGAGGATT CCAAAGAGCT CCGGCTAATA GGCATGAAGC TGGGCTGCCT GCTGGCCCGC
    AAAGGCTGCG TGGAGAAGAT GCAGTATTAC TGGGATGTGG GTTTCTACCT GGGAGCCCAG
    ATCCTCGCCA ATGACCCCAC CCAGGTGGTG CTGGCTGCAG AGCAGCTGTA TAAGCTCAAT
    GCCCCCATAT GGTACCTGGT GTCCGTGATG GAGACCTTCC TGCTCTACCA GCACTTCAGG
    CCCACGCCAG AGCCCCCTGG AGGGCCACCA CGCCGTGCCC ACTTCTGGCT CCACTTCTTG
    CTACAGTCCT GCCAACCATT CAAGACAGCC TGTGCCCAGG GCGACCAGTG CTTGGTGCTG
    GTCCTGGAGA TGAACAAGGT GCTGCTGCCT GCAAAGCTCG AGGTTCGGGG TACTGACCCA
    GTAAGCACAG TGACCCTGAG CCTGCTGGAG CCTGAGACCC AGGACATTCC CTCCAGCTGG
    ACCTTCCCAG TCGCCTCCAT ATGCGGAGTC AGCGCCTCAA AGCGCGACGA GCGCTGCTGC
    TTCCTCTATG CACTCCCCCC GGCTCAGGAC GTCCAGCTGT GCTTCCCCAG CGTAGGGCAC
    TGCCAGTGGT TCTGCGGCCT GATCCAGGCC TGGGTGACGA ACCCGGATTC CACGGCGCCC
    GCGGAGGAGG CGGAGGGCGC GGGGGAGATG TTGGAGTTTG ATTATGAGTA CACGGAGACG
    GGCGAGCGGC TGGTGCTGGG CAAGGGCACG TATGGGGTGG TGTACGCGGG CCGCGATCGC
    CACACGAGGG TGCGCATCGC CATCAAGGAG ATCCCGGAGC GGGACAGCAG GTTCTCTCAG
    CCCCTGCATG AAGAGATCGC TCTTCACAGA CGCCTGCGCC ACAAGAACAT AGTGCGCTAT
    CTGGGCTCAG CTAGCCAGGG CGGCTACCTT AAGATCTTCA TGGAGGAAGT GCCTGGAGGC
    AGCCTGTCCT CCTTGCTGCG GTCGGTGTGG GGACCCCTGA AGGACAACGA GAGCACCATC
    AGTTTCTACA CCCGCCAGAT CCTGCAGGGA CTTGGCTACT TGCACGACAA CCACATCGTG
    CACAGGGACA TAAAAGGGGA CAATGTGCTG ATCAACACCT TCAGTGGGCT GCTCAAGATT
    TCTGACTTCG GCACCTCCAA GCGGCTGGCA GGCATCACAC CTTGCACTGA GACCTTCACA
    GGAACTCTGC AGTATATGGC CCCAGAAATC ATTGACCAGG GCCCACGCGG GTATGGGAAA
    GCAGCTGACA TCTGGTCACT GGGCTGCACT GTCATTGAGA TGGCCACAGG TCGCCCCCCC
    TTCCACGAGC TCGGGAGCCC ACAGGCTGCC ATGTTTCAGG TGGGTATGTA CAAGGTCCAT
    CCGCCAATGC CCAGCTCTCT GTCGGCCGAG GCCCAAGCCT TTCTCCTCCG AACTTTTGAG
    CCAGACCCCC GCCTCCGAGC CAGCGCCCAG ACACTGCTGG GGGACCCCTT CCTGCAGCCT
    GGGAAAAGGA GCCGCAGCCC CAGCTCCCCA CGACATGCTC CACGGCCCTC AGATGCCCCT
    TCTGCCAGTC CCACTCCTTC AGCCAACTCA ACCACCCAGT CTCAGACATT CCCGTGCCCT
    CAGGCACCCT CTCAGCACCC ACCCAGCCCC CCGAAGCGCT GCCTCAGTTA TGGGGGCACC
    AGCCAGCTCC GGGTGCCCGA GGAGCCTGCG GCCGAGGAGC CTGCGTCTCC GGAGGAGAGT
    TCGGGGCTGA GCCTGCTGCA CCAGGAGAGC AAGCGTCGGG CCATGCTGGC CGCAGTATTG
    GAGCAGGAGC TGCCAGCGCT GGCGGAGAAT CTGCACCAGG AGCAGAAGCA AGAGCAGGGG
    GCCCGTCTGG GCAGAAACCA TGTGGAAGAG CTGCTGCGCT GCCTCGGGGC ACACATCCAC
    ACTCCCAACC GCCGGCAGCT CGCCCAGGAG CTGCGGGCGC TGCAAGGACG GCTGAGGGCC
    CAGGGCCTTG GGCCTGCGCT TCTGCACAGA CCGCTGTTTG CCTTCCCGGA TGCGGTGAAG
    CAGATCCTCC GCAAGCGCCA GATCCGTCCA CACTGGATGT TCGTTCTGGA CTCACTGCTC
    AGCCGTGCTG TGCGGGCAGC CCTGGGTGTG CTAGGACCGG AGGTGGAGAA GGAGGCGGTC
    TCACCGAGGT CAGAGGAGCT GAGTAATGAA GGGGACTCCC AGCAGAGCCC AGGCCAGCAG
    AGCCCGCTTC CGGTGGAGCC CGAGCAGGGC CCCGCTCCTC TGATGGTGCA GCTGAGCCTC
    TTGAGGGCAG AGACTGATCG GCTGCGCGAA ATCCTGGCGG GGAAGGAACG GGAGTACCAG
    GCCCTGGTGC AGCGGGCTCT ACAGCGGCTG AATGAGGAAG CCCGGACCTA TGTCCTGGCC
    CCAGAGCCTC CAACTGCTCT TTCAACGGAC CAGGGCCTGG TGCAGTGGCT ACAGGAACTG
    AATGTGGATT CAGGCACCAT CCAAATGCTG TTGAACCATA GCTTCACCCT CCACACTCTG
    CTCACCTATG CCACTCGAGA TGACCTCATC TACACCCGCA TCAGGGGAGG GATGGTATGC
    CGCATCTGGA GGGCCATCTT GGCACAGCGA GCAGGATCCA CACCAGTCAC CTCTGGACCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284538.1
    CDS365..4207
    Translation

    Target ORF information:

    RefSeq Version NM_001297609.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001297609.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GATTGCGTTG GCAGCTACAC ACTGATCCCC TATGTGGTGA CGGCCACTGG TCGGGTGCTG
    TGTGGTGATG CAGGCCTTCT GCGGGGCCTG GCTGATGGGC TGGTACAGGC TGGAGTGGGG
    ACCGAGGCCC TGCTCACTCC CCTGGTGGGC CGGCTTGCCC GCCTGCTGGA GGCCACACCC
    ACAGACTCTT GTGGCTATTT CCGGGAGACC ATTCGGCGGG ACATCCGGCA GGCGCGGGAG
    CGGTTCAGTG GGCCACAGCT GCGGCAGGAG CTGGCTCGCC TGCAGCGGAG ACTGGACAGC
    GTGGAGCTGC TGAGCCCCGA CATCATCATG AACTTGCTGC TCTCCTACCG CGATGTGCAG
    GACTACTCGG CCATCATTGA GCTGGTGGAG ACGCTGCAGG CCTTGCCCAC CTGTGATGTG
    GCCGAGCAGC ATAATGTCTG CTTCCACTAC ACTTTTGCCC TCAACCGGAG GAACAGGCCT
    GGGGACCGGG CGAAGGCCCT GTCTGTGCTG CTGCCGCTGG TACAGCTTGA GGGCTCTGTG
    GCGCCCGATC TGTACTGCAT GTGTGGCCGT ATCTACAAGG ACATGTTCTT CAGCTCGGGT
    TTCCAGGATG CTGGGCACCG GGAGCAGGCC TATCACTGGT ATCGCAAGGC TTTTGACGTA
    GAGCCCAGCC TTCACTCAGG CATCAATGCA GCTGTGCTCC TCATTGCTGC CGGGCAGCAC
    TTTGAGGATT CCAAAGAGCT CCGGCTAATA GGCATGAAGC TGGGCTGCCT GCTGGCCCGC
    AAAGGCTGCG TGGAGAAGAT GCAGTATTAC TGGGATGTGG GTTTCTACCT GGGAGCCCAG
    ATCCTCGCCA ATGACCCCAC CCAGGTGGTG CTGGCTGCAG AGCAGCTGTA TAAGCTCAAT
    GCCCCCATAT GGTACCTGGT GTCCGTGATG GAGACCTTCC TGCTCTACCA GCACTTCAGG
    CCCACGCCAG AGCCCCCTGG AGGGCCACCA CGCCGTGCCC ACTTCTGGCT CCACTTCTTG
    CTACAGTCCT GCCAACCATT CAAGACAGCC TGTGCCCAGG GCGACCAGTG CTTGGTGCTG
    GTCCTGGAGA TGAACAAGGT GCTGCTGCCT GCAAAGCTCG AGGTTCGGGG TACTGACCCA
    GTAAGCACAG TGACCCTGAG CCTGCTGGAG CCTGAGACCC AGGACATTCC CTCCAGCTGG
    ACCTTCCCAG TCGCCTCCAT ATGCGGAGTC AGCGCCTCAA AGCGCGACGA GCGCTGCTGC
    TTCCTCTATG CACTCCCCCC GGCTCAGGAC GTCCAGCTGT GCTTCCCCAG CGTAGGGCAC
    TGCCAGTGGT TCTGCGGCCT GATCCAGGCC TGGGTGACGA ACCCGGATTC CACGGCGCCC
    GCGGAGGAGG CGGAGGGCGC GGGGGAGATG TTGGAGTTTG ATTATGAGTA CACGGAGACG
    GGCGAGCGGC TGGTGCTGGG CAAGGGCACG TATGGGGTGG TGTACGCGGG CCGCGATCGC
    CACACGAGGG TGCGCATCGC CATCAAGGAG ATCCCGGAGC GGGACAGCAG GTTCTCTCAG
    CCCCTGCATG AAGAGATCGC TCTTCACAGA CGCCTGCGCC ACAAGAACAT AGTGCGCTAT
    CTGGGCTCAG CTAGCCAGGG CGGCTACCTT AAGATCTTCA TGGAGGAAGT GCCTGGAGGC
    AGCCTGTCCT CCTTGCTGCG GTCGGTGTGG GGACCCCTGA AGGACAACGA GAGCACCATC
    AGTTTCTACA CCCGCCAGAT CCTGCAGGGA CTTGGCTACT TGCACGACAA CCACATCGTG
    CACAGGGACA TAAAAGGGGA CAATGTGCTG ATCAACACCT TCAGTGGGCT GCTCAAGATT
    TCTGACTTCG GCACCTCCAA GCGGCTGGCA GGCATCACAC CTTGCACTGA GACCTTCACA
    GGAACTCTGC AGTATATGGC CCCAGAAATC ATTGACCAGG GCCCACGCGG GTATGGGAAA
    GCAGCTGACA TCTGGTCACT GGGCTGCACT GTCATTGAGA TGGCCACAGG TCGCCCCCCC
    TTCCACGAGC TCGGGAGCCC ACAGGCTGCC ATGTTTCAGG TGGGTATGTA CAAGGTCCAT
    CCGCCAATGC CCAGCTCTCT GTCGGCCGAG GCCCAAGCCT TTCTCCTCCG AACTTTTGAG
    CCAGACCCCC GCCTCCGAGC CAGCGCCCAG ACACTGCTGG GGGACCCCTT CCTGCAGCCT
    GGGAAAAGGA GCCGCAGCCC CAGCTCCCCA CGACATGCTC CACGGCCCTC AGATGCCCCT
    TCTGCCAGTC CCACTCCTTC AGCCAACTCA ACCACCCAGT CTCAGACATT CCCGTGCCCT
    CAGGCACCCT CTCAGCACCC ACCCAGCCCC CCGAAGCGCT GCCTCAGTTA TGGGGGCACC
    AGCCAGCTCC GGGTGCCCGA GGAGCCTGCG GCCGAGGAGC CTGCGTCTCC GGAGGAGAGT
    TCGGGGCTGA GCCTGCTGCA CCAGGAGAGC AAGCGTCGGG CCATGCTGGC CGCAGTATTG
    GAGCAGGAGC TGCCAGCGCT GGCGGAGAAT CTGCACCAGG AGCAGAAGCA AGAGCAGGGG
    GCCCGTCTGG GCAGAAACCA TGTGGAAGAG CTGCTGCGCT GCCTCGGGGC ACACATCCAC
    ACTCCCAACC GCCGGCAGCT CGCCCAGGAG CTGCGGGCGC TGCAAGGACG GCTGAGGGCC
    CAGGGCCTTG GGCCTGCGCT TCTGCACAGA CCGCTGTTTG CCTTCCCGGA TGCGGTGAAG
    CAGATCCTCC GCAAGCGCCA GATCCGTCCA CACTGGATGT TCGTTCTGGA CTCACTGCTC
    AGCCGTGCTG TGCGGGCAGC CCTGGGTGTG CTAGGACCGG AGGTGGAGAA GGAGGCGGTC
    TCACCGAGGT CAGAGGAGCT GAGTAATGAA GGGGACTCCC AGCAGAGCCC AGGCCAGCAG
    AGCCCGCTTC CGGTGGAGCC CGAGCAGGGC CCCGCTCCTC TGATGGTGCA GCTGAGCCTC
    TTGAGGGCAG AGACTGATCG GCTGCGCGAA ATCCTGGCGG GGAAGGAACG GGAGTACCAG
    GCCCTGGTGC AGCGGGCTCT ACAGCGGCTG AATGAGGAAG CCCGGACCTA TGTCCTGGCC
    CCAGAGCCTC CAACTGCTCT TTCAACGGAC CAGGGCCTGG TGCAGTGGCT ACAGGAACTG
    AATGTGGATT CAGGCACCAT CCAAATGCTG TTGAACCATA GCTTCACCCT CCACACTCTG
    CTCACCTATG CCACTCGAGA TGACCTCATC TACACCCGCA TCAGGGGAGG GATGGTATGC
    CGCATCTGGA GGGCCATCTT GGCACAGCGA GCAGGATCCA CACCAGTCAC CTCTGGACCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28868
    Clone ID Related Accession (Same CDS sequence) NM_001297609.2 , NM_001297609.1
    Accession Version NM_001297609.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 6 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW771625.1, AB208805.1, FO393419.1 and AI658745.1. On Jul 4, 2014 this sequence version replaced XM_005246029.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB208805.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GATTGCGTTG GCAGCTACAC ACTGATCCCC TATGTGGTGA CGGCCACTGG TCGGGTGCTG
    TGTGGTGATG CAGGCCTTCT GCGGGGCCTG GCTGATGGGC TGGTACAGGC TGGAGTGGGG
    ACCGAGGCCC TGCTCACTCC CCTGGTGGGC CGGCTTGCCC GCCTGCTGGA GGCCACACCC
    ACAGACTCTT GTGGCTATTT CCGGGAGACC ATTCGGCGGG ACATCCGGCA GGCGCGGGAG
    CGGTTCAGTG GGCCACAGCT GCGGCAGGAG CTGGCTCGCC TGCAGCGGAG ACTGGACAGC
    GTGGAGCTGC TGAGCCCCGA CATCATCATG AACTTGCTGC TCTCCTACCG CGATGTGCAG
    GACTACTCGG CCATCATTGA GCTGGTGGAG ACGCTGCAGG CCTTGCCCAC CTGTGATGTG
    GCCGAGCAGC ATAATGTCTG CTTCCACTAC ACTTTTGCCC TCAACCGGAG GAACAGGCCT
    GGGGACCGGG CGAAGGCCCT GTCTGTGCTG CTGCCGCTGG TACAGCTTGA GGGCTCTGTG
    GCGCCCGATC TGTACTGCAT GTGTGGCCGT ATCTACAAGG ACATGTTCTT CAGCTCGGGT
    TTCCAGGATG CTGGGCACCG GGAGCAGGCC TATCACTGGT ATCGCAAGGC TTTTGACGTA
    GAGCCCAGCC TTCACTCAGG CATCAATGCA GCTGTGCTCC TCATTGCTGC CGGGCAGCAC
    TTTGAGGATT CCAAAGAGCT CCGGCTAATA GGCATGAAGC TGGGCTGCCT GCTGGCCCGC
    AAAGGCTGCG TGGAGAAGAT GCAGTATTAC TGGGATGTGG GTTTCTACCT GGGAGCCCAG
    ATCCTCGCCA ATGACCCCAC CCAGGTGGTG CTGGCTGCAG AGCAGCTGTA TAAGCTCAAT
    GCCCCCATAT GGTACCTGGT GTCCGTGATG GAGACCTTCC TGCTCTACCA GCACTTCAGG
    CCCACGCCAG AGCCCCCTGG AGGGCCACCA CGCCGTGCCC ACTTCTGGCT CCACTTCTTG
    CTACAGTCCT GCCAACCATT CAAGACAGCC TGTGCCCAGG GCGACCAGTG CTTGGTGCTG
    GTCCTGGAGA TGAACAAGGT GCTGCTGCCT GCAAAGCTCG AGGTTCGGGG TACTGACCCA
    GTAAGCACAG TGACCCTGAG CCTGCTGGAG CCTGAGACCC AGGACATTCC CTCCAGCTGG
    ACCTTCCCAG TCGCCTCCAT ATGCGGAGTC AGCGCCTCAA AGCGCGACGA GCGCTGCTGC
    TTCCTCTATG CACTCCCCCC GGCTCAGGAC GTCCAGCTGT GCTTCCCCAG CGTAGGGCAC
    TGCCAGTGGT TCTGCGGCCT GATCCAGGCC TGGGTGACGA ACCCGGATTC CACGGCGCCC
    GCGGAGGAGG CGGAGGGCGC GGGGGAGATG TTGGAGTTTG ATTATGAGTA CACGGAGACG
    GGCGAGCGGC TGGTGCTGGG CAAGGGCACG TATGGGGTGG TGTACGCGGG CCGCGATCGC
    CACACGAGGG TGCGCATCGC CATCAAGGAG ATCCCGGAGC GGGACAGCAG GTTCTCTCAG
    CCCCTGCATG AAGAGATCGC TCTTCACAGA CGCCTGCGCC ACAAGAACAT AGTGCGCTAT
    CTGGGCTCAG CTAGCCAGGG CGGCTACCTT AAGATCTTCA TGGAGGAAGT GCCTGGAGGC
    AGCCTGTCCT CCTTGCTGCG GTCGGTGTGG GGACCCCTGA AGGACAACGA GAGCACCATC
    AGTTTCTACA CCCGCCAGAT CCTGCAGGGA CTTGGCTACT TGCACGACAA CCACATCGTG
    CACAGGGACA TAAAAGGGGA CAATGTGCTG ATCAACACCT TCAGTGGGCT GCTCAAGATT
    TCTGACTTCG GCACCTCCAA GCGGCTGGCA GGCATCACAC CTTGCACTGA GACCTTCACA
    GGAACTCTGC AGTATATGGC CCCAGAAATC ATTGACCAGG GCCCACGCGG GTATGGGAAA
    GCAGCTGACA TCTGGTCACT GGGCTGCACT GTCATTGAGA TGGCCACAGG TCGCCCCCCC
    TTCCACGAGC TCGGGAGCCC ACAGGCTGCC ATGTTTCAGG TGGGTATGTA CAAGGTCCAT
    CCGCCAATGC CCAGCTCTCT GTCGGCCGAG GCCCAAGCCT TTCTCCTCCG AACTTTTGAG
    CCAGACCCCC GCCTCCGAGC CAGCGCCCAG ACACTGCTGG GGGACCCCTT CCTGCAGCCT
    GGGAAAAGGA GCCGCAGCCC CAGCTCCCCA CGACATGCTC CACGGCCCTC AGATGCCCCT
    TCTGCCAGTC CCACTCCTTC AGCCAACTCA ACCACCCAGT CTCAGACATT CCCGTGCCCT
    CAGGCACCCT CTCAGCACCC ACCCAGCCCC CCGAAGCGCT GCCTCAGTTA TGGGGGCACC
    AGCCAGCTCC GGGTGCCCGA GGAGCCTGCG GCCGAGGAGC CTGCGTCTCC GGAGGAGAGT
    TCGGGGCTGA GCCTGCTGCA CCAGGAGAGC AAGCGTCGGG CCATGCTGGC CGCAGTATTG
    GAGCAGGAGC TGCCAGCGCT GGCGGAGAAT CTGCACCAGG AGCAGAAGCA AGAGCAGGGG
    GCCCGTCTGG GCAGAAACCA TGTGGAAGAG CTGCTGCGCT GCCTCGGGGC ACACATCCAC
    ACTCCCAACC GCCGGCAGCT CGCCCAGGAG CTGCGGGCGC TGCAAGGACG GCTGAGGGCC
    CAGGGCCTTG GGCCTGCGCT TCTGCACAGA CCGCTGTTTG CCTTCCCGGA TGCGGTGAAG
    CAGATCCTCC GCAAGCGCCA GATCCGTCCA CACTGGATGT TCGTTCTGGA CTCACTGCTC
    AGCCGTGCTG TGCGGGCAGC CCTGGGTGTG CTAGGACCGG AGGTGGAGAA GGAGGCGGTC
    TCACCGAGGT CAGAGGAGCT GAGTAATGAA GGGGACTCCC AGCAGAGCCC AGGCCAGCAG
    AGCCCGCTTC CGGTGGAGCC CGAGCAGGGC CCCGCTCCTC TGATGGTGCA GCTGAGCCTC
    TTGAGGGCAG AGACTGATCG GCTGCGCGAA ATCCTGGCGG GGAAGGAACG GGAGTACCAG
    GCCCTGGTGC AGCGGGCTCT ACAGCGGCTG AATGAGGAAG CCCGGACCTA TGTCCTGGCC
    CCAGAGCCTC CAACTGCTCT TTCAACGGAC CAGGGCCTGG TGCAGTGGCT ACAGGAACTG
    AATGTGGATT CAGGCACCAT CCAAATGCTG TTGAACCATA GCTTCACCCT CCACACTCTG
    CTCACCTATG CCACTCGAGA TGACCTCATC TACACCCGCA TCAGGGGAGG GATGGTATGC
    CGCATCTGGA GGGCCATCTT GGCACAGCGA GCAGGATCCA CACCAGTCAC CTCTGGACCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284538.1
    CDS270..4112
    Translation

    Target ORF information:

    RefSeq Version NM_001297609.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001297609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GATTGCGTTG GCAGCTACAC ACTGATCCCC TATGTGGTGA CGGCCACTGG TCGGGTGCTG
    TGTGGTGATG CAGGCCTTCT GCGGGGCCTG GCTGATGGGC TGGTACAGGC TGGAGTGGGG
    ACCGAGGCCC TGCTCACTCC CCTGGTGGGC CGGCTTGCCC GCCTGCTGGA GGCCACACCC
    ACAGACTCTT GTGGCTATTT CCGGGAGACC ATTCGGCGGG ACATCCGGCA GGCGCGGGAG
    CGGTTCAGTG GGCCACAGCT GCGGCAGGAG CTGGCTCGCC TGCAGCGGAG ACTGGACAGC
    GTGGAGCTGC TGAGCCCCGA CATCATCATG AACTTGCTGC TCTCCTACCG CGATGTGCAG
    GACTACTCGG CCATCATTGA GCTGGTGGAG ACGCTGCAGG CCTTGCCCAC CTGTGATGTG
    GCCGAGCAGC ATAATGTCTG CTTCCACTAC ACTTTTGCCC TCAACCGGAG GAACAGGCCT
    GGGGACCGGG CGAAGGCCCT GTCTGTGCTG CTGCCGCTGG TACAGCTTGA GGGCTCTGTG
    GCGCCCGATC TGTACTGCAT GTGTGGCCGT ATCTACAAGG ACATGTTCTT CAGCTCGGGT
    TTCCAGGATG CTGGGCACCG GGAGCAGGCC TATCACTGGT ATCGCAAGGC TTTTGACGTA
    GAGCCCAGCC TTCACTCAGG CATCAATGCA GCTGTGCTCC TCATTGCTGC CGGGCAGCAC
    TTTGAGGATT CCAAAGAGCT CCGGCTAATA GGCATGAAGC TGGGCTGCCT GCTGGCCCGC
    AAAGGCTGCG TGGAGAAGAT GCAGTATTAC TGGGATGTGG GTTTCTACCT GGGAGCCCAG
    ATCCTCGCCA ATGACCCCAC CCAGGTGGTG CTGGCTGCAG AGCAGCTGTA TAAGCTCAAT
    GCCCCCATAT GGTACCTGGT GTCCGTGATG GAGACCTTCC TGCTCTACCA GCACTTCAGG
    CCCACGCCAG AGCCCCCTGG AGGGCCACCA CGCCGTGCCC ACTTCTGGCT CCACTTCTTG
    CTACAGTCCT GCCAACCATT CAAGACAGCC TGTGCCCAGG GCGACCAGTG CTTGGTGCTG
    GTCCTGGAGA TGAACAAGGT GCTGCTGCCT GCAAAGCTCG AGGTTCGGGG TACTGACCCA
    GTAAGCACAG TGACCCTGAG CCTGCTGGAG CCTGAGACCC AGGACATTCC CTCCAGCTGG
    ACCTTCCCAG TCGCCTCCAT ATGCGGAGTC AGCGCCTCAA AGCGCGACGA GCGCTGCTGC
    TTCCTCTATG CACTCCCCCC GGCTCAGGAC GTCCAGCTGT GCTTCCCCAG CGTAGGGCAC
    TGCCAGTGGT TCTGCGGCCT GATCCAGGCC TGGGTGACGA ACCCGGATTC CACGGCGCCC
    GCGGAGGAGG CGGAGGGCGC GGGGGAGATG TTGGAGTTTG ATTATGAGTA CACGGAGACG
    GGCGAGCGGC TGGTGCTGGG CAAGGGCACG TATGGGGTGG TGTACGCGGG CCGCGATCGC
    CACACGAGGG TGCGCATCGC CATCAAGGAG ATCCCGGAGC GGGACAGCAG GTTCTCTCAG
    CCCCTGCATG AAGAGATCGC TCTTCACAGA CGCCTGCGCC ACAAGAACAT AGTGCGCTAT
    CTGGGCTCAG CTAGCCAGGG CGGCTACCTT AAGATCTTCA TGGAGGAAGT GCCTGGAGGC
    AGCCTGTCCT CCTTGCTGCG GTCGGTGTGG GGACCCCTGA AGGACAACGA GAGCACCATC
    AGTTTCTACA CCCGCCAGAT CCTGCAGGGA CTTGGCTACT TGCACGACAA CCACATCGTG
    CACAGGGACA TAAAAGGGGA CAATGTGCTG ATCAACACCT TCAGTGGGCT GCTCAAGATT
    TCTGACTTCG GCACCTCCAA GCGGCTGGCA GGCATCACAC CTTGCACTGA GACCTTCACA
    GGAACTCTGC AGTATATGGC CCCAGAAATC ATTGACCAGG GCCCACGCGG GTATGGGAAA
    GCAGCTGACA TCTGGTCACT GGGCTGCACT GTCATTGAGA TGGCCACAGG TCGCCCCCCC
    TTCCACGAGC TCGGGAGCCC ACAGGCTGCC ATGTTTCAGG TGGGTATGTA CAAGGTCCAT
    CCGCCAATGC CCAGCTCTCT GTCGGCCGAG GCCCAAGCCT TTCTCCTCCG AACTTTTGAG
    CCAGACCCCC GCCTCCGAGC CAGCGCCCAG ACACTGCTGG GGGACCCCTT CCTGCAGCCT
    GGGAAAAGGA GCCGCAGCCC CAGCTCCCCA CGACATGCTC CACGGCCCTC AGATGCCCCT
    TCTGCCAGTC CCACTCCTTC AGCCAACTCA ACCACCCAGT CTCAGACATT CCCGTGCCCT
    CAGGCACCCT CTCAGCACCC ACCCAGCCCC CCGAAGCGCT GCCTCAGTTA TGGGGGCACC
    AGCCAGCTCC GGGTGCCCGA GGAGCCTGCG GCCGAGGAGC CTGCGTCTCC GGAGGAGAGT
    TCGGGGCTGA GCCTGCTGCA CCAGGAGAGC AAGCGTCGGG CCATGCTGGC CGCAGTATTG
    GAGCAGGAGC TGCCAGCGCT GGCGGAGAAT CTGCACCAGG AGCAGAAGCA AGAGCAGGGG
    GCCCGTCTGG GCAGAAACCA TGTGGAAGAG CTGCTGCGCT GCCTCGGGGC ACACATCCAC
    ACTCCCAACC GCCGGCAGCT CGCCCAGGAG CTGCGGGCGC TGCAAGGACG GCTGAGGGCC
    CAGGGCCTTG GGCCTGCGCT TCTGCACAGA CCGCTGTTTG CCTTCCCGGA TGCGGTGAAG
    CAGATCCTCC GCAAGCGCCA GATCCGTCCA CACTGGATGT TCGTTCTGGA CTCACTGCTC
    AGCCGTGCTG TGCGGGCAGC CCTGGGTGTG CTAGGACCGG AGGTGGAGAA GGAGGCGGTC
    TCACCGAGGT CAGAGGAGCT GAGTAATGAA GGGGACTCCC AGCAGAGCCC AGGCCAGCAG
    AGCCCGCTTC CGGTGGAGCC CGAGCAGGGC CCCGCTCCTC TGATGGTGCA GCTGAGCCTC
    TTGAGGGCAG AGACTGATCG GCTGCGCGAA ATCCTGGCGG GGAAGGAACG GGAGTACCAG
    GCCCTGGTGC AGCGGGCTCT ACAGCGGCTG AATGAGGAAG CCCGGACCTA TGTCCTGGCC
    CCAGAGCCTC CAACTGCTCT TTCAACGGAC CAGGGCCTGG TGCAGTGGCT ACAGGAACTG
    AATGTGGATT CAGGCACCAT CCAAATGCTG TTGAACCATA GCTTCACCCT CCACACTCTG
    CTCACCTATG CCACTCGAGA TGACCTCATC TACACCCGCA TCAGGGGAGG GATGGTATGC
    CGCATCTGGA GGGCCATCTT GGCACAGCGA GCAGGATCCA CACCAGTCAC CTCTGGACCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90785
    Clone ID Related Accession (Same CDS sequence) XM_017002772.1
    Accession Version XM_017002772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GAGGATGTTT TCCAGAAGAA CTCGGATTGC GTTGGCAGCT ACACACTGAT CCCCTATGTG
    GTGACGGCCA CTGGTCGGGT GCTGTGTGGT GATGCAGGCC TTCTGCGGGG CCTGGCTGAT
    GGGCTGGTAC AGGCTGGAGT GGGGACCGAG GCCCTGCTCA CTCCCCTGGT GGGCCGGCTT
    GCCCGCCTGC TGGAGGCCAC ACCCACAGAC TCTTGTGGCT ATTTCCGGGA GACCATTCGG
    CGGGACATCC GGCAGGCGCG GGAGCGGTTC AGTGGGCCAC AGCTGCGGCA GGAGCTGGCT
    CGCCTGCAGC GGAGACTGGA CAGCGTGGAG CTGCTGAGCC CCGACATCAT CATGAACTTG
    CTGCTCTCCT ACCGCGATGT GCAGGACTAC TCGGCCATCA TTGAGCTGGT GGAGACGCTG
    CAGGCCTTGC CCACCTGTGA TGTGGCCGAG CAGCATAATG TCTGCTTCCA CTACACTTTT
    GCCCTCAACC GGAGGAACAG GCCTGGGGAC CGGGCGAAGG CCCTGTCTGT GCTGCTGCCG
    CTGGTACAGC TTGAGGGCTC TGTGGCGCCC GATCTGTACT GCATGTGTGG CCGTATCTAC
    AAGGACATGT TCTTCAGCTC GGGTTTCCAG GATGCTGGGC ACCGGGAGCA GGCCTATCAC
    TGGTATCGCA AGGCTTTTGA CGTAGAGCCC AGCCTTCACT CAGGCATCAA TGCAGCTGTG
    CTCCTCATTG CTGCCGGGCA GCACTTTGAG GATTCCAAAG AGCTCCGGCT AATAGGCATG
    AAGCTGGGCT GCCTGCTGGC CCGCAAAGGC TGCGTGGAGA AGATGCAGTA TTACTGGGAT
    GTGGGTTTCT ACCTGGGAGC CCAGATCCTC GCCAATGACC CCACCCAGGT GGTGCTGGCT
    GCAGAGCAGC TGTATAAGCT CAATGCCCCC ATATGGTACC TGGTGTCCGT GATGGAGACC
    TTCCTGCTCT ACCAGCACTT CAGGCCCACG CCAGAGCCCC CTGGAGGGCC ACCACGCCGT
    GCCCACTTCT GGCTCCACTT CTTGCTACAG TCCTGCCAAC CATTCAAGAC AGCCTGTGCC
    CAGGGCGACC AGTGCTTGGT GCTGGTCCTG GAGATGAACA AGGTGCTGCT GCCTGCAAAG
    CTCGAGGTTC GGGGTACTGA CCCAGTAAGC ACAGTGACCC TGAGCCTGCT GGAGCCTGAG
    ACCCAGGACA TTCCCTCCAG CTGGACCTTC CCAGTCGCCT CCATATGCGG AGTCAGCGCC
    TCAAAGCGCG ACGAGCGCTG CTGCTTCCTC TATGCACTCC CCCCGGCTCA GGACGTCCAG
    CTGTGCTTCC CCAGCGTAGG GCACTGCCAG TGGTTCTGCG GCCTGATCCA GGCCTGGGTG
    ACGAACCCGG ATTCCACGGC GCCCGCGGAG GAGGCGGAGG GCGCGGGGGA GATGTTGGAG
    TTTGATTATG AGTACACGGA GACGGGCGAG CGGCTGGTGC TGGGCAAGGG CACGTATGGG
    GTGGTGTACG CGGGCCGCGA TCGCCACACG AGGGTGCGCA TCGCCATCAA GGAGATCCCG
    GAGCGGGACA GCAGGTTCTC TCAGCCCCTG CATGAAGAGA TCGCTCTTCA CAGACGCCTG
    CGCCACAAGA ACATAGTGCG CTATCTGGGC TCAGCTAGCC AGGGCGGCTA CCTTAAGATC
    TTCATGGAGG AAGTGCCTGG AGGCAGCCTG TCCTCCTTGC TGCGGTCGGT GTGGGGACCC
    CTGAAGGACA ACGAGAGCAC CATCAGTTTC TACACCCGCC AGATCCTGCA GGGACTTGGC
    TACTTGCACG ACAACCACAT CGTGCACAGG GACATAAAAG GGCCCAGACC AGTGTCCACT
    AATGGCTGTC AATCAGGGGG ACAATGTGCT GATCAACACC TTCAGTGGGC TGCTCAAGAT
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858261.1
    CDS1441..3846
    Translation

    Target ORF information:

    RefSeq Version XM_017002772.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGGGC CGTGTCCCCG GTCCGGGGCG GAGCGCGCCG GCAGCTGCTG GCAGGACCCG 
    CTGGCCGTGG CGCTGAGCCG GGGCCGGCAG CTCGCGGCGC CCCCGGGCCG GGGCTGCGCG
    CGGAGCCGGC CGCTCAGCGT GGTCTACGTG CTGACCCGGG AGCCGCAGCC CGGGCTCGAG
    CCTCGGGAGG GAACCGAGGC GGAGCCGCTG CCCCTGCGCT GCCTGCGCGA GGCTTGCGCG
    CAGGTCCCCC GGCCGCGGCC GCCCCCGCAG CTGCGCAGCC TGCCCTTCGG GACGCTGGAG
    CTAGGCGACA CCGCGGCTCT GGATGCCTTC TACAACGCGG ATGTGGTGGT GCTGGAGGTG
    AGCAGCTCGC TGGTACAGCC CTCCCTGTTC TACCACCTTG GTGTGCGTGA GAGCTTCAGC
    ATGACCAACA ATGTGCTCCT CTGCTCCCAG GCCGACCTCC CTGACCTGCA GGCCCTGCGG
    GAGGATGTTT TCCAGAAGAA CTCGGATTGC GTTGGCAGCT ACACACTGAT CCCCTATGTG
    GTGACGGCCA CTGGTCGGGT GCTGTGTGGT GATGCAGGCC TTCTGCGGGG CCTGGCTGAT
    GGGCTGGTAC AGGCTGGAGT GGGGACCGAG GCCCTGCTCA CTCCCCTGGT GGGCCGGCTT
    GCCCGCCTGC TGGAGGCCAC ACCCACAGAC TCTTGTGGCT ATTTCCGGGA GACCATTCGG
    CGGGACATCC GGCAGGCGCG GGAGCGGTTC AGTGGGCCAC AGCTGCGGCA GGAGCTGGCT
    CGCCTGCAGC GGAGACTGGA CAGCGTGGAG CTGCTGAGCC CCGACATCAT CATGAACTTG
    CTGCTCTCCT ACCGCGATGT GCAGGACTAC TCGGCCATCA TTGAGCTGGT GGAGACGCTG
    CAGGCCTTGC CCACCTGTGA TGTGGCCGAG CAGCATAATG TCTGCTTCCA CTACACTTTT
    GCCCTCAACC GGAGGAACAG GCCTGGGGAC CGGGCGAAGG CCCTGTCTGT GCTGCTGCCG
    CTGGTACAGC TTGAGGGCTC TGTGGCGCCC GATCTGTACT GCATGTGTGG CCGTATCTAC
    AAGGACATGT TCTTCAGCTC GGGTTTCCAG GATGCTGGGC ACCGGGAGCA GGCCTATCAC
    TGGTATCGCA AGGCTTTTGA CGTAGAGCCC AGCCTTCACT CAGGCATCAA TGCAGCTGTG
    CTCCTCATTG CTGCCGGGCA GCACTTTGAG GATTCCAAAG AGCTCCGGCT AATAGGCATG
    AAGCTGGGCT GCCTGCTGGC CCGCAAAGGC TGCGTGGAGA AGATGCAGTA TTACTGGGAT
    GTGGGTTTCT ACCTGGGAGC CCAGATCCTC GCCAATGACC CCACCCAGGT GGTGCTGGCT
    GCAGAGCAGC TGTATAAGCT CAATGCCCCC ATATGGTACC TGGTGTCCGT GATGGAGACC
    TTCCTGCTCT ACCAGCACTT CAGGCCCACG CCAGAGCCCC CTGGAGGGCC ACCACGCCGT
    GCCCACTTCT GGCTCCACTT CTTGCTACAG TCCTGCCAAC CATTCAAGAC AGCCTGTGCC
    CAGGGCGACC AGTGCTTGGT GCTGGTCCTG GAGATGAACA AGGTGCTGCT GCCTGCAAAG
    CTCGAGGTTC GGGGTACTGA CCCAGTAAGC ACAGTGACCC TGAGCCTGCT GGAGCCTGAG
    ACCCAGGACA TTCCCTCCAG CTGGACCTTC CCAGTCGCCT CCATATGCGG AGTCAGCGCC
    TCAAAGCGCG ACGAGCGCTG CTGCTTCCTC TATGCACTCC CCCCGGCTCA GGACGTCCAG
    CTGTGCTTCC CCAGCGTAGG GCACTGCCAG TGGTTCTGCG GCCTGATCCA GGCCTGGGTG
    ACGAACCCGG ATTCCACGGC GCCCGCGGAG GAGGCGGAGG GCGCGGGGGA GATGTTGGAG
    TTTGATTATG AGTACACGGA GACGGGCGAG CGGCTGGTGC TGGGCAAGGG CACGTATGGG
    GTGGTGTACG CGGGCCGCGA TCGCCACACG AGGGTGCGCA TCGCCATCAA GGAGATCCCG
    GAGCGGGACA GCAGGTTCTC TCAGCCCCTG CATGAAGAGA TCGCTCTTCA CAGACGCCTG
    CGCCACAAGA ACATAGTGCG CTATCTGGGC TCAGCTAGCC AGGGCGGCTA CCTTAAGATC
    TTCATGGAGG AAGTGCCTGG AGGCAGCCTG TCCTCCTTGC TGCGGTCGGT GTGGGGACCC
    CTGAAGGACA ACGAGAGCAC CATCAGTTTC TACACCCGCC AGATCCTGCA GGGACTTGGC
    TACTTGCACG ACAACCACAT CGTGCACAGG GACATAAAAG GGCCCAGACC AGTGTCCACT
    AATGGCTGTC AATCAGGGGG ACAATGTGCT GATCAACACC TTCAGTGGGC TGCTCAAGAT
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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