ORF cDNA Clones and Custom Clones » Species Summary » Homo sapiens » PDLIM1 cDNA ORF clone

PDLIM1 cDNA ORF clone, Homo sapiens(human)

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Summary

Gene Symbol	PDLIM1
Entrez Gene ID	9124
Full Name	PDZ and LIM domain 1
Synonyms	CLIM1,CLP-36,CLP36,HEL-S-112,hCLIM1
General protein information	Preferred Names PDZ and LIM domain 1 Names PDZ and LIM domain protein 1 LIM domain protein CLP-36 carboxyl terminal LIM domain protein 1 elfin epididymis secretory protein Li 112
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.33
Summary	This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012].
Disorder MIM:	605900

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_020992.4	NP_066272.1	PDZ and LIM domain protein 1
NM_020992.3	NP_066272.1	PDZ and LIM domain protein 1
XM_011540330.1	XP_011538632.1	PDZ and LIM domain protein 1 isoform X1

Homologies

	PDLIM1	XP_001099155.1
Bos taurus (cattle)	PDLIM1	NP_001030532.1
Gallus gallus (chicken)	PDLIM1	XP_426503.4
Danio rerio (zebrafish)	pdlim1	NP_001017870.1
Pan troglodytes (chimpanzee)	PDLIM1	XP_001153448.1
Mus musculus (house mouse)	Pdlim1	NP_058557.2
Xenopus tropicalis (tropical clawed frog)	pdlim1	NP_988930.1
Homo sapiens (human)	PDLIM1	NP_066272.1
Rattus norvegicus (Norway rat)	Pdlim1	NP_059061.1
Canis lupus familiaris (dog)	PDLIM1	XP_534974.4

Bibliography

Related articles in PubMed

Autoantibodies against TYMS and PDLIM1 proteins detected as circulatory signatures in Indian breast cancer patients.
Gupta P, Suman S, Mishra M, Mishra S, Srivastava N, Kumar V, Singh PK, Shukla Y
Proteomics. Clinical applications10(5)564-73(2016 05)

Glioma invasion mediated by the p75 neurotrophin receptor (p75(NTR)/CD271) requires regulated interaction with PDLIM1.
Ahn BY, Saldanha-Gama RF, Rahn JJ, Hao X, Zhang J, Dang NH, Alshehri M, Robbins SM, Senger DL
Oncogene35(11)1411-22(2016 Mar)

PDLIM1 Stabilizes the E-Cadherin/?-Catenin Complex to Prevent Epithelial-Mesenchymal Transition and Metastatic Potential of Colorectal Cancer Cells.
Chen HN, Yuan K, Xie N, Wang K, Huang Z, Chen Y, Dou Q, Wu M, Nice EC, Zhou ZG, Huang C
Cancer research76(5)1122-34(2016 Mar)

PDZ and LIM domain protein 1(PDLIM1)/CLP36 promotes breast cancer cell migration, invasion and metastasis through interaction with ?-actinin.
Liu Z, Zhan Y, Tu Y, Chen K, Liu Z, Wu C
Oncogene34(10)1300-11(2015 Mar)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High titers of both TYMS and PDLIM1 serum autoantibodies were significantly more prevalent in breast cancer cases than in controls.
Title: Autoantibodies against TYMS and PDLIM1 proteins detected as circulatory signatures in Indian breast cancer patients.

PDLIM1 was shown to interact with p75(NTR) in highly invasive patient-derived glioma stem cells/tumor-initiating cells and shRNA knockdown of PDLIM1 in vitro and in vivo results in complete ablation of p75(NTR)-mediated invasion.
Title: Glioma invasion mediated by the p75 neurotrophin receptor (p75(NTR)/CD271) requires regulated interaction with PDLIM1.

downregulation of PDLIM1 in colorectal cancer samples correlated with reduced E-cadherin and membrane beta-catenin levels
Title: PDLIM1 Stabilizes the E-Cadherin/?-Catenin Complex to Prevent Epithelial-Mesenchymal Transition and Metastatic Potential of Colorectal Cancer Cells.

Our results identify CLP36 as an important regulator of breast cancer cell migration and metastasis, and shed light on how increased CLP36 expression contributes to the progression of breast cancer.
Title: PDZ and LIM domain protein 1(PDLIM1)/CLP36 promotes breast cancer cell migration, invasion and metastasis through interaction with ?-actinin.

Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability.
Title: Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability.

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The following PDLIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDLIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01736	NM_020992.4 Latest version!	Homo sapiens PDZ and LIM domain 1 (PDLIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu62491	XM_011540330.1 Latest version!	Homo sapiens PDZ and LIM domain 1 (PDLIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OHu01736	NM_020992.3	Homo sapiens PDZ and LIM domain 1 (PDLIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDLIM1 ( NM_020992.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066272.1 Homo sapiens PDZ and LIM domain 1 (PDLIM1), mRNA.

Price & Availability↑

CloneID	OHu01736
Clone ID Related Accession (Same CDS sequence)	NM_020992.4 , NM_020992.3
Accession Version	NM_020992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	PDZ and LIM domain protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC000915.2 and AL160288.21. On Nov 22, 2018 this sequence version replaced NM_020992.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.305224.1, BC000915.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329399.7/ ENSP00000360305.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCACCC AGCAGATAGA CCTCCAGGGC CCGGGGCCGT GGGGCTTCCG CCTCGTGGGC 
GGCAAGGACT TCGAGCAGCC TCTCGCCATT TCCCGGGTCA CTCCTGGAAG CAAGGCGGCT 
CTAGCTAATT TATGTATTGG AGATGTAATC ACAGCCATTG ATGGGGAAAA TACTAGCAAT 
ATGACACACT TGGAAGCTCA GAACAGAATC AAAGGCTGCA CAGACAACTT GACTCTCACT 
GTAGCCAGAT CTGAACATAA AGTCTGGTCT CCTCTGGTGA CGGAGGAAGG GAAGCGTCAT 
CCATACAAGA TGAATTTAGC CTCTGAACCC CAGGAGGTCC TGCACATAGG AAGCGCCCAC 
AACCGAAGTG CCATGCCCTT TACCGCCTCG CCTGCCTCCA GCACTACTGC CAGGGTCATC 
ACAAACCAGT ACAACAACCC AGCTGGCCTC TACTCTTCTG AAAATATCTC CAACTTCAAC 
AATGCCCTGG AGTCAAAGAC TGCTGCCAGC GGGGTGGAGG CGAACAGCAG ACCCTTAGAC 
CATGCTCAGC CTCCAAGCAG CCTTGTCATC GACAAAGAAT CTGAAGTTTA CAAGATGCTT 
CAGGAGAAAC AGGAGTTGAA TGAGCCCCCG AAACAGTCCA CGTCTTTCTT GGTTTTGCAG 
GAAATCCTGG AGTCTGAAGA AAAAGGGGAT CCCAACAAGC CCTCAGGATT CAGAAGTGTT 
AAAGCTCCTG TCACTAAAGT GGCTGCGTCG ATTGGAAATG CTCAGAAGTT GCCTATGTGT 
GACAAATGTG GCACTGGGAT TGTTGGTGTG TTTGTGAAGC TGCGGGACCG TCACCGCCAC 
CCTGAGTGTT ATGTGTGCAC TGACTGTGGC ACCAACCTGA AACAGAAGGG CCATTTCTTT 
GTGGAGGATC AAATCTACTG TGAGAAGCAT GCCCGGGAGC GAGTCACACC ACCTGAGGGT 
TATGAAGTGG TCACTGTGTT CCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066272.1
CDS	89..1078
Translation	MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK

Target ORF information:

RefSeq Version	NM_020992.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PDZ and LIM domain 1 (PDLIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020992.4

ORF Insert Sequence:

ATGACCACCC AGCAGATAGA CCTCCAGGGC CCGGGGCCGT GGGGCTTCCG CCTCGTGGGC 
GGCAAGGACT TCGAGCAGCC TCTCGCCATT TCCCGGGTCA CTCCTGGAAG CAAGGCGGCT 
CTAGCTAATT TATGTATTGG AGATGTAATC ACAGCCATTG ATGGGGAAAA TACTAGCAAT 
ATGACACACT TGGAAGCTCA GAACAGAATC AAAGGCTGCA CAGACAACTT GACTCTCACT 
GTAGCCAGAT CTGAACATAA AGTCTGGTCT CCTCTGGTGA CGGAGGAAGG GAAGCGTCAT 
CCATACAAGA TGAATTTAGC CTCTGAACCC CAGGAGGTCC TGCACATAGG AAGCGCCCAC 
AACCGAAGTG CCATGCCCTT TACCGCCTCG CCTGCCTCCA GCACTACTGC CAGGGTCATC 
ACAAACCAGT ACAACAACCC AGCTGGCCTC TACTCTTCTG AAAATATCTC CAACTTCAAC 
AATGCCCTGG AGTCAAAGAC TGCTGCCAGC GGGGTGGAGG CGAACAGCAG ACCCTTAGAC 
CATGCTCAGC CTCCAAGCAG CCTTGTCATC GACAAAGAAT CTGAAGTTTA CAAGATGCTT 
CAGGAGAAAC AGGAGTTGAA TGAGCCCCCG AAACAGTCCA CGTCTTTCTT GGTTTTGCAG 
GAAATCCTGG AGTCTGAAGA AAAAGGGGAT CCCAACAAGC CCTCAGGATT CAGAAGTGTT 
AAAGCTCCTG TCACTAAAGT GGCTGCGTCG ATTGGAAATG CTCAGAAGTT GCCTATGTGT 
GACAAATGTG GCACTGGGAT TGTTGGTGTG TTTGTGAAGC TGCGGGACCG TCACCGCCAC 
CCTGAGTGTT ATGTGTGCAC TGACTGTGGC ACCAACCTGA AACAGAAGGG CCATTTCTTT 
GTGGAGGATC AAATCTACTG TGAGAAGCAT GCCCGGGAGC GAGTCACACC ACCTGAGGGT 
TATGAAGTGG TCACTGTGTT CCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDLIM1 ( NM_020992.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066272.1 Homo sapiens PDZ and LIM domain 1 (PDLIM1), mRNA.

Price & Availability↑

CloneID	OHu01736
Clone ID Related Accession (Same CDS sequence)	NM_020992.4 , NM_020992.3
Accession Version	NM_020992.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	PDZ and LIM domain protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY025482.1, AL160288.21, BC000915.2 and CD368928.1. On Oct 24, 2012 this sequence version replaced NM_020992.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.202976.1, SRR1163655.150351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACCC AGCAGATAGA CCTCCAGGGC CCGGGGCCGT GGGGCTTCCG CCTCGTGGGC 
GGCAAGGACT TCGAGCAGCC TCTCGCCATT TCCCGGGTCA CTCCTGGAAG CAAGGCGGCT 
CTAGCTAATT TATGTATTGG AGATGTAATC ACAGCCATTG ATGGGGAAAA TACTAGCAAT 
ATGACACACT TGGAAGCTCA GAACAGAATC AAAGGCTGCA CAGACAACTT GACTCTCACT 
GTAGCCAGAT CTGAACATAA AGTCTGGTCT CCTCTGGTGA CGGAGGAAGG GAAGCGTCAT 
CCATACAAGA TGAATTTAGC CTCTGAACCC CAGGAGGTCC TGCACATAGG AAGCGCCCAC 
AACCGAAGTG CCATGCCCTT TACCGCCTCG CCTGCCTCCA GCACTACTGC CAGGGTCATC 
ACAAACCAGT ACAACAACCC AGCTGGCCTC TACTCTTCTG AAAATATCTC CAACTTCAAC 
AATGCCCTGG AGTCAAAGAC TGCTGCCAGC GGGGTGGAGG CGAACAGCAG ACCCTTAGAC 
CATGCTCAGC CTCCAAGCAG CCTTGTCATC GACAAAGAAT CTGAAGTTTA CAAGATGCTT 
CAGGAGAAAC AGGAGTTGAA TGAGCCCCCG AAACAGTCCA CGTCTTTCTT GGTTTTGCAG 
GAAATCCTGG AGTCTGAAGA AAAAGGGGAT CCCAACAAGC CCTCAGGATT CAGAAGTGTT 
AAAGCTCCTG TCACTAAAGT GGCTGCGTCG ATTGGAAATG CTCAGAAGTT GCCTATGTGT 
GACAAATGTG GCACTGGGAT TGTTGGTGTG TTTGTGAAGC TGCGGGACCG TCACCGCCAC 
CCTGAGTGTT ATGTGTGCAC TGACTGTGGC ACCAACCTGA AACAGAAGGG CCATTTCTTT 
GTGGAGGATC AAATCTACTG TGAGAAGCAT GCCCGGGAGC GAGTCACACC ACCTGAGGGT 
TATGAAGTGG TCACTGTGTT CCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066272.1
CDS	234..1223
Translation	MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK

Target ORF information:

RefSeq Version	NM_020992.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PDZ and LIM domain 1 (PDLIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020992.3

ORF Insert Sequence:

ATGACCACCC AGCAGATAGA CCTCCAGGGC CCGGGGCCGT GGGGCTTCCG CCTCGTGGGC 
GGCAAGGACT TCGAGCAGCC TCTCGCCATT TCCCGGGTCA CTCCTGGAAG CAAGGCGGCT 
CTAGCTAATT TATGTATTGG AGATGTAATC ACAGCCATTG ATGGGGAAAA TACTAGCAAT 
ATGACACACT TGGAAGCTCA GAACAGAATC AAAGGCTGCA CAGACAACTT GACTCTCACT 
GTAGCCAGAT CTGAACATAA AGTCTGGTCT CCTCTGGTGA CGGAGGAAGG GAAGCGTCAT 
CCATACAAGA TGAATTTAGC CTCTGAACCC CAGGAGGTCC TGCACATAGG AAGCGCCCAC 
AACCGAAGTG CCATGCCCTT TACCGCCTCG CCTGCCTCCA GCACTACTGC CAGGGTCATC 
ACAAACCAGT ACAACAACCC AGCTGGCCTC TACTCTTCTG AAAATATCTC CAACTTCAAC 
AATGCCCTGG AGTCAAAGAC TGCTGCCAGC GGGGTGGAGG CGAACAGCAG ACCCTTAGAC 
CATGCTCAGC CTCCAAGCAG CCTTGTCATC GACAAAGAAT CTGAAGTTTA CAAGATGCTT 
CAGGAGAAAC AGGAGTTGAA TGAGCCCCCG AAACAGTCCA CGTCTTTCTT GGTTTTGCAG 
GAAATCCTGG AGTCTGAAGA AAAAGGGGAT CCCAACAAGC CCTCAGGATT CAGAAGTGTT 
AAAGCTCCTG TCACTAAAGT GGCTGCGTCG ATTGGAAATG CTCAGAAGTT GCCTATGTGT 
GACAAATGTG GCACTGGGAT TGTTGGTGTG TTTGTGAAGC TGCGGGACCG TCACCGCCAC 
CCTGAGTGTT ATGTGTGCAC TGACTGTGGC ACCAACCTGA AACAGAAGGG CCATTTCTTT 
GTGGAGGATC AAATCTACTG TGAGAAGCAT GCCCGGGAGC GAGTCACACC ACCTGAGGGT 
TATGAAGTGG TCACTGTGTT CCCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDLIM1 ( XM_011540330.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538632.1 Homo sapiens PDZ and LIM domain 1 (PDLIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu62491
Clone ID Related Accession (Same CDS sequence)	XM_011540330.1
Accession Version	XM_011540330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PDZ and LIM domain protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTAG CCTCTGAACC CCAGGAGGTC CTGCACATAG GAAGCGCCCA CAACCGAAGT 
GCCATGCCCT TTACCGCCTC GCCTGCCTCC AGCACTACTG CCAGGGTCAT CACAAACCAG 
TACAACAACC CAGCTGGCCT CTACTCTTCT GAAAATATCT CCAACTTCAA CAATGCCCTG 
GAGTCAAAGA CTGCTGCCAG CGGGGTGGAG GCGAACAGCA GACCCTTAGA CCATGCTCAG 
CCTCCAAGCA GCCTTGTCAT CGACAAAGAA TCTGAAGTTT ACAAGATGCT TCAGGAGAAA 
CAGGAGTTGA ATGAGCCCCC GAAACAGTCC ACGTCTTTCT TGGTTTTGCA GGAAATCCTG 
GAGTCTGAAG AAAAAGGGGA TCCCAACAAG CCCTCAGGAT TCAGAAGTGT TAAAGCTCCT 
GTCACTAAAG TGGCTGCGTC GATTGGAAAT GCTCAGAAGT TGCCTATGTG TGACAAATGT 
GGCACTGGGA TTGTTGGTGT GTTTGTGAAG CTGCGGGACC GTCACCGCCA CCCTGAGTGT 
TATGTGTGCA CTGACTGTGG CACCAACCTG AAACAGAAGG GCCATTTCTT TGTGGAGGAT 
CAAATCTACT GTGAGAAGCA TGCCCGGGAG CGAGTCACAC CACCTGAGGG TTATGAAGTG 
GTCACTGTGT TCCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538632.1
CDS	249..929
Translation	MNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK

Target ORF information:

RefSeq Version	XM_011540330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PDZ and LIM domain 1 (PDLIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540330.1

ORF Insert Sequence:

ATGAATTTAG CCTCTGAACC CCAGGAGGTC CTGCACATAG GAAGCGCCCA CAACCGAAGT 
GCCATGCCCT TTACCGCCTC GCCTGCCTCC AGCACTACTG CCAGGGTCAT CACAAACCAG 
TACAACAACC CAGCTGGCCT CTACTCTTCT GAAAATATCT CCAACTTCAA CAATGCCCTG 
GAGTCAAAGA CTGCTGCCAG CGGGGTGGAG GCGAACAGCA GACCCTTAGA CCATGCTCAG 
CCTCCAAGCA GCCTTGTCAT CGACAAAGAA TCTGAAGTTT ACAAGATGCT TCAGGAGAAA 
CAGGAGTTGA ATGAGCCCCC GAAACAGTCC ACGTCTTTCT TGGTTTTGCA GGAAATCCTG 
GAGTCTGAAG AAAAAGGGGA TCCCAACAAG CCCTCAGGAT TCAGAAGTGT TAAAGCTCCT 
GTCACTAAAG TGGCTGCGTC GATTGGAAAT GCTCAGAAGT TGCCTATGTG TGACAAATGT 
GGCACTGGGA TTGTTGGTGT GTTTGTGAAG CTGCGGGACC GTCACCGCCA CCCTGAGTGT 
TATGTGTGCA CTGACTGTGG CACCAACCTG AAACAGAAGG GCCATTTCTT TGTGGAGGAT 
CAAATCTACT GTGAGAAGCA TGCCCGGGAG CGAGTCACAC CACCTGAGGG TTATGAAGTG 
GTCACTGTGT TCCCCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.