• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

SPAG6 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SPAG6
Entrez Gene ID 9576
Full Name sperm associated antigen 6
Synonyms CT141,Repro-SA-1,pf16
General protein information
Preferred Names

sperm associated antigen 6

Names

sperm-associated antigen 6
axoneme central apparatus protein
protein PF16 homolog
sperm flagellar protein
testicular tissue protein Li 176
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

10

10p12.2
Summary The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011].
Disorder MIM:

605730


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_172242.2 NP_758442.1 sperm-associated antigen 6 isoform 2
XM_005252645.1 XP_005252702.1 sperm-associated antigen 6 isoform X1
NM_172242.3 NP_758442.1 sperm-associated antigen 6 isoform 2
NM_001253854.2 NP_001240783.1 sperm-associated antigen 6 isoform 3
XM_005252646.1 XP_005252703.1 sperm-associated antigen 6 isoform X2
XM_005252646.2 XP_005252703.1 sperm-associated antigen 6 isoform X2
NM_012443.3 NP_036575.1 sperm-associated antigen 6 isoform 1
NM_001253854.1 NP_001240783.1 sperm-associated antigen 6 isoform 3
NM_012443.4 NP_036575.1 sperm-associated antigen 6 isoform 1
XM_005252645.2 XP_005252702.1 sperm-associated antigen 6 isoform X1
NM_001253855.1 NP_001240784.1 sperm-associated antigen 6 isoform 4

Pathways from BioSystems


Homologies


Homo sapiens (human) SPAG6 NP_036575.1
Gallus gallus (chicken) SPAG6 NP_001186515.1
Danio rerio (zebrafish) spag6 NP_001002210.1
Xenopus tropicalis (tropical clawed frog) spag6 NP_001015976.1
SPAG6 XP_001097347.1
Canis lupus familiaris (dog) SPAG6 XP_535163.3
Rattus norvegicus (Norway rat) Spag6 NP_001030132.1
Pan troglodytes (chimpanzee) SPAG6 NP_001233340.1
Bos taurus (cattle) SPAG6 NP_001033274.1
Mus musculus (house mouse) Spag6 NP_056588.1

Gene Ontology


Bibliography

Related articles in PubMed

SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes.
Li X, Yang B, Wang L, Chen L, Luo X, Liu L
Oncology reports37(5)2839-2846(2017 May)

SPAG6 and L1TD1 are transcriptionally regulated by DNA methylation in non-small cell lung cancers.
Altenberger C, Heller G, Ziegler B, Tomasich E, Marhold M, Topakian T, M?llauer L, Heffeter P, Lang G, End-Pf?tzenreuter A, D?me B, Arns BM, Klepetko W, Zielinski CC, Z?chbauer-M?ller S
Molecular cancer16(1)1(2017 01)

SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis.
Yang B, Wang L, Luo X, Chen L, Yang Z, Liu L
International journal of oncology46(2)649-56(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrates that SPAG6 may regulate apoptosis through the TRAIL signal pathway by inhibiting the expression of FADD and the interactions between FADD and the TRAIL death receptors.
Title: SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes.

SPAG6 and L1TD1 are tumor-specifically methylated in NSCLCs and that DNA methylation is involved in the transcriptional regulation of these genes. Moreover, in vitro as well as in vivo experiments revealed tumor-cell growth suppressing properties of L1TD1 in NSCLC cells.
Title: SPAG6 and L1TD1 are transcriptionally regulated by DNA methylation in non-small cell lung cancers.

These findings demonstrate that SPAG6 might have a role in malignant myeloid hematologic cell proliferation and apoptosis by regulating caspase proteins and p53, suggesting that SPAG6 may be a potential therapeutic target.
Title: SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis.

studies demonstrate a role for Spag6 in the pathogenesis of OM in mice, possibly through its role in the regulation of cilia/basal body polarity through the PCP-dependent mechanisms in the middle ear and Eustachian tubes.
Title: Otitis media in sperm-associated antigen 6 (Spag6)-deficient mice.

SPAG6 is a S-SOX5 target gene, indicating a key role for S-SOX5 in the formation and function of motile cilia.
Title: Transcriptional regulation of an axonemal central apparatus gene, sperm-associated antigen 6, by a SRY-related high mobility group transcription factor, S-SOX5.

The following SPAG6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu07170 NM_172242.3
Latest version!		 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu29207 NM_001253855.1
Latest version!		 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu29215 NM_001253854.2
Latest version!		 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu29216 NM_012443.4
Latest version!		 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $300.30
$429.00
OHu43907 XM_005252645.2
Latest version!		 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu43908 XM_005252646.2
Latest version!		 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu07170 NM_172242.2 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu43908 XM_005252646.1 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu29215 NM_001253854.1 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu29216 NM_012443.3 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $300.30
$429.00
OHu43907 XM_005252645.1 Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu07170
Clone ID Related Accession (Same CDS sequence) NM_172242.2 , NM_172242.3
Accession Version NM_172242.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-10-12
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB057157.1, DB025758.1, AL080136.1, BC030585.2 and AF079363.1. On Dec 8, 2011 this sequence version replaced NM_172242.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030585.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTATTA 
TTCCCCTGGA TATTCAGATA CACTTCTGCA GAGGGTGGAC AGCTATCAAC CACTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_758442.1
CDS168..1544
Translation

Target ORF information:

RefSeq Version NM_172242.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_172242.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTATTA 
TTCCCCTGGA TATTCAGATA CACTTCTGCA GAGGGTGGAC AGCTATCAAC CACTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu07170
Clone ID Related Accession (Same CDS sequence) NM_172242.2 , NM_172242.3
Accession Version NM_172242.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-27
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025758.1, AL080136.1, BC030585.2 and AF079363.1. On May 31, 2019 this sequence version replaced NM_172242.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030585.2, SRR5189667.128759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTATTA 
TTCCCCTGGA TATTCAGATA CACTTCTGCA GAGGGTGGAC AGCTATCAAC CACTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_758442.1
CDS117..1493
Translation

Target ORF information:

RefSeq Version NM_172242.3
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_172242.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTATTA 
TTCCCCTGGA TATTCAGATA CACTTCTGCA GAGGGTGGAC AGCTATCAAC CACTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29207
Clone ID Related Accession (Same CDS sequence) NM_001253855.1
Accession Version NM_001253855.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-23
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 4
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB057157.1, DB025758.1, AK310247.1, AF079363.1 and BC030585.2. Transcript Variant: This variant (4) retains an intron in the 5' end and contains an alternate coding exon in the 3' end. The resulting isoform (4) uses a downstream translation start codon, so it has a shorter N-terminus and a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTGGCGG AGCTGGCGAC TAGACCCCAA AACATCGAGA CGCTGCAGAA CGCGGGTGTA 
ATGTCTTTGC TGAGAACTCT TCTTCTGGAC GTGGTCCCAA CAATTCAACA GACTGCTGCT 
TTGGCTCTTG GGAGACTGGC CAATTATAAT GATGACCTAG CAGAAGCTGT TGTGAAGTGC 
GACATTCTTC CACAGCTTGT TTATTCATTG GCAGAACAGA ATCGCTTCTA CAAGAAAGCA 
GCTGCCTTTG TGTTACGAGC AGTTGGTAAA CATTCTCCCC AGCTAGCTCA GGCAATAGTC 
GATTGTGGAG CACTGGATAC GCTGGTCATA TGCTTGGAAG ATTTTGACCC TGGAGTCAAG 
GAGGCTGCAG CCTGGGCACT TAGATATATT GCAAGACATA ATGCAGAACT GTCACAAGCT 
GTGGTGGATG CAGGAGCTGT TCCTCTTTTA GTACTCTGTA TCCAGGAGCC AGAAATTGCT 
TTGAAAAGGA TTGCTGCTTC GGCCCTCAGT GATATTGCAA AGCATTCTCC AGAGTTAGCA 
CAGACAGTAG TGGATGCAGG AGCTGTTGCT CATTTAGCCC AGATGATCCT GAACCCTGAT 
GCTAAATTGA AGCATCAGAT CCTTTCAGCT CTCAGTCAGG TTTCAAAACA TTCCGTGGAT 
CTGGCAGAAA TGGTTGTTGA AGCAGAGATT TTTCCAGTTG TACTTACCTG TCTGAAGGAC 
AAGGATGAAT ACGTGAAGAA AAATGCTTCT ACTTTAATTA GAGAGATTGC AAAACATACA 
CCCGAGCTTT CACAGCTGGT AGTTAACGCA GGAGGGGTTG CTGCCGTGAT TGACTGCATT 
GGGTCCTGCA AAGGGAACAC ACGGCTGCCT GGCATCATGA TGCTTGGTTA TGTAGCAGCT 
CATTCTGAGA ACCTAGCAAT GGCAGTCATC ATTTCTAAGG GTGTACCCCA GTTGTCAGTC 
TGCTTGTCAG AAGAACCGGA AGATCATATT AAGGCTGCAG CTGCTTGGGC CTTAGGACAG 
ATTGGAAGAC ACACTCCTGA ACACGCACGG GCTGTTGCAG TCACAAATAC TTTGCCAGTT 
CTGCTTTCTT TGTACATGTC AACAGAAAGT TCTGAGGATC TCCAAGTAAA AAGTAAAAAA 
GCCATAAAGA ATATCCTGCA AAAATGTACC TACTTACCAG CCCTTGAACC ATTTCTATAT 
GATGCTCCTC CCAATATTCT GAAACATGTG GTTGGACAGT TCAGTAAGGT GCTGCCGCAT 
GATAGCAAAG CTCGACGACT TTTTGTAACA AGTGGTGGCC TTAAAAAAGT TCAAGAGATA 
AAAGCAGAAC CTGGTTCTCT CCTTCAAGAA TACATCAACA GTATTAACAG TTGTTACCCC 
GAGGAAATAG TGAGTAAATT CTTTCCAGAG TTGCAAGTTT GTGCACTTTT TAGGAAGTTT 
ATGAGAACTC CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001240784.1
CDS320..1774
Translation

Target ORF information:

RefSeq Version NM_001253855.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001253855.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTGGCGG AGCTGGCGAC TAGACCCCAA AACATCGAGA CGCTGCAGAA CGCGGGTGTA 
ATGTCTTTGC TGAGAACTCT TCTTCTGGAC GTGGTCCCAA CAATTCAACA GACTGCTGCT 
TTGGCTCTTG GGAGACTGGC CAATTATAAT GATGACCTAG CAGAAGCTGT TGTGAAGTGC 
GACATTCTTC CACAGCTTGT TTATTCATTG GCAGAACAGA ATCGCTTCTA CAAGAAAGCA 
GCTGCCTTTG TGTTACGAGC AGTTGGTAAA CATTCTCCCC AGCTAGCTCA GGCAATAGTC 
GATTGTGGAG CACTGGATAC GCTGGTCATA TGCTTGGAAG ATTTTGACCC TGGAGTCAAG 
GAGGCTGCAG CCTGGGCACT TAGATATATT GCAAGACATA ATGCAGAACT GTCACAAGCT 
GTGGTGGATG CAGGAGCTGT TCCTCTTTTA GTACTCTGTA TCCAGGAGCC AGAAATTGCT 
TTGAAAAGGA TTGCTGCTTC GGCCCTCAGT GATATTGCAA AGCATTCTCC AGAGTTAGCA 
CAGACAGTAG TGGATGCAGG AGCTGTTGCT CATTTAGCCC AGATGATCCT GAACCCTGAT 
GCTAAATTGA AGCATCAGAT CCTTTCAGCT CTCAGTCAGG TTTCAAAACA TTCCGTGGAT 
CTGGCAGAAA TGGTTGTTGA AGCAGAGATT TTTCCAGTTG TACTTACCTG TCTGAAGGAC 
AAGGATGAAT ACGTGAAGAA AAATGCTTCT ACTTTAATTA GAGAGATTGC AAAACATACA 
CCCGAGCTTT CACAGCTGGT AGTTAACGCA GGAGGGGTTG CTGCCGTGAT TGACTGCATT 
GGGTCCTGCA AAGGGAACAC ACGGCTGCCT GGCATCATGA TGCTTGGTTA TGTAGCAGCT 
CATTCTGAGA ACCTAGCAAT GGCAGTCATC ATTTCTAAGG GTGTACCCCA GTTGTCAGTC 
TGCTTGTCAG AAGAACCGGA AGATCATATT AAGGCTGCAG CTGCTTGGGC CTTAGGACAG 
ATTGGAAGAC ACACTCCTGA ACACGCACGG GCTGTTGCAG TCACAAATAC TTTGCCAGTT 
CTGCTTTCTT TGTACATGTC AACAGAAAGT TCTGAGGATC TCCAAGTAAA AAGTAAAAAA 
GCCATAAAGA ATATCCTGCA AAAATGTACC TACTTACCAG CCCTTGAACC ATTTCTATAT 
GATGCTCCTC CCAATATTCT GAAACATGTG GTTGGACAGT TCAGTAAGGT GCTGCCGCAT 
GATAGCAAAG CTCGACGACT TTTTGTAACA AGTGGTGGCC TTAAAAAAGT TCAAGAGATA 
AAAGCAGAAC CTGGTTCTCT CCTTCAAGAA TACATCAACA GTATTAACAG TTGTTACCCC 
GAGGAAATAG TGAGTAAATT CTTTCCAGAG TTGCAAGTTT GTGCACTTTT TAGGAAGTTT 
ATGAGAACTC CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29215
Clone ID Related Accession (Same CDS sequence) NM_001253854.2 , NM_001253854.1
Accession Version NM_001253854.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-28
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302194.1, AF079363.1 and BC030585.2. On Jun 1, 2019 this sequence version replaced NM_001253854.1. Transcript Variant: This variant (3) contains several alternate exons in the 5' end that cause a frameshift and use of a downstream translation start site. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2161674 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCATTCAC TCACCATGGA TCTCTGGATA CCTAATGGAC AGGCAGGTGT AATGTCTTTG 
CTGAGAACTC TTCTTCTGGA CGTGGTCCCA ACAATTCAAC AGACTGCTGC TTTGGCTCTT 
GGGAGACTGG CCAATTATAA TGATGACCTA GCAGAAGCTG TTGTGAAGTG CGACATTCTT 
CCACAGCTTG TTTATTCATT GGCAGAACAG AATCGCTTCT ACAAGAAAGC AGCTGCCTTT 
GTGTTACGAG CAGTTGGTAA ACATTCTCCC CAGCTAGCTC AGGCAATAGT CGATTGTGGA 
GCACTGGATA CGCTGGTCAT ATGCTTGGAA GATTTTGACC CTGGAGTCAA GGAGGCTGCA 
GCCTGGGCAC TTAGATATAT TGCAAGACAT AATGCAGAAC TGTCACAAGC TGTGGTGGAT 
GCAGGAGCTG TTCCTCTTTT AGTACTCTGT ATCCAGGAGC CAGAAATTGC TTTGAAAAGG 
ATTGCTGCTT CGGCCCTCAG TGATATTGCA AAGCATTCTC CAGAGTTAGC ACAGACAGTA 
GTGGATGCAG GAGCTGTTGC TCATTTAGCC CAGATGATCC TGAACCCTGA TGCTAAATTG 
AAGCATCAGA TCCTTTCAGC TCTCAGTCAG GTTTCAAAAC ATTCCGTGGA TCTGGCAGAA 
ATGGTTGTTG AAGCAGAGAT TTTTCCAGTT GTACTTACCT GTCTGAAGGA CAAGGATGAA 
TACGTGAAGA AAAATGCTTC TACTTTAATT AGAGAGATTG CAAAACATAC ACCCGAGCTT 
TCACAGCTGG TAGTTAACGC AGGAGGGGTT GCTGCCGTGA TTGACTGCAT TGGGTCCTGC 
AAAGGGAACA CACGGCTGCC TGGCATCATG ATGCTTGGTT ATGTAGCAGC TCATTCTGAG 
AACCTAGCAA TGGCAGTCAT CATTTCTAAG GGTGTACCCC AGTTGTCAGT CTGCTTGTCA 
GAAGAACCGG AAGATCATAT TAAGGCTGCA GCTGCTTGGG CCTTAGGACA GATTGGAAGA 
CACACTCCTG AACACGCACG GGCTGTTGCA GTCACAAATA CTTTGCCAGT TCTGCTTTCT 
TTGTACATGT CAACAGAAAG TTCTGAGGAT CTCCAAGTAA AAAGTAAAAA AGCCATAAAG 
AATATCCTGC AAAAATGTAC CTACTTACCA GCCCTTGAAC CATTTCTATA TGATGCTCCT 
CCCAATATTC TGAAACATGT GGTTGGACAG TTCAGTAAGG TGCTGCCGCA TGATAGCAAA 
GCTCGACGAC TTTTTGTAAC AAGTGGTGGC CTTAAAAAAG TTCAAGAGAT AAAAGCAGAA 
CCTGGTTCTC TCCTTCAAGA ATACATCAAC AGTATTAACA GTTGTTACCC CGAGGAAATA 
GTGAGGTATT ATTCCCCTGG ATATTCAGAT ACACTTCTGC AGAGGGTGGA CAGCTATCAA 
CCACTTAATA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001240783.1
CDS547..2001
Translation

Target ORF information:

RefSeq Version NM_001253854.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001253854.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCATTCAC TCACCATGGA TCTCTGGATA CCTAATGGAC AGGCAGGTGT AATGTCTTTG 
CTGAGAACTC TTCTTCTGGA CGTGGTCCCA ACAATTCAAC AGACTGCTGC TTTGGCTCTT 
GGGAGACTGG CCAATTATAA TGATGACCTA GCAGAAGCTG TTGTGAAGTG CGACATTCTT 
CCACAGCTTG TTTATTCATT GGCAGAACAG AATCGCTTCT ACAAGAAAGC AGCTGCCTTT 
GTGTTACGAG CAGTTGGTAA ACATTCTCCC CAGCTAGCTC AGGCAATAGT CGATTGTGGA 
GCACTGGATA CGCTGGTCAT ATGCTTGGAA GATTTTGACC CTGGAGTCAA GGAGGCTGCA 
GCCTGGGCAC TTAGATATAT TGCAAGACAT AATGCAGAAC TGTCACAAGC TGTGGTGGAT 
GCAGGAGCTG TTCCTCTTTT AGTACTCTGT ATCCAGGAGC CAGAAATTGC TTTGAAAAGG 
ATTGCTGCTT CGGCCCTCAG TGATATTGCA AAGCATTCTC CAGAGTTAGC ACAGACAGTA 
GTGGATGCAG GAGCTGTTGC TCATTTAGCC CAGATGATCC TGAACCCTGA TGCTAAATTG 
AAGCATCAGA TCCTTTCAGC TCTCAGTCAG GTTTCAAAAC ATTCCGTGGA TCTGGCAGAA 
ATGGTTGTTG AAGCAGAGAT TTTTCCAGTT GTACTTACCT GTCTGAAGGA CAAGGATGAA 
TACGTGAAGA AAAATGCTTC TACTTTAATT AGAGAGATTG CAAAACATAC ACCCGAGCTT 
TCACAGCTGG TAGTTAACGC AGGAGGGGTT GCTGCCGTGA TTGACTGCAT TGGGTCCTGC 
AAAGGGAACA CACGGCTGCC TGGCATCATG ATGCTTGGTT ATGTAGCAGC TCATTCTGAG 
AACCTAGCAA TGGCAGTCAT CATTTCTAAG GGTGTACCCC AGTTGTCAGT CTGCTTGTCA 
GAAGAACCGG AAGATCATAT TAAGGCTGCA GCTGCTTGGG CCTTAGGACA GATTGGAAGA 
CACACTCCTG AACACGCACG GGCTGTTGCA GTCACAAATA CTTTGCCAGT TCTGCTTTCT 
TTGTACATGT CAACAGAAAG TTCTGAGGAT CTCCAAGTAA AAAGTAAAAA AGCCATAAAG 
AATATCCTGC AAAAATGTAC CTACTTACCA GCCCTTGAAC CATTTCTATA TGATGCTCCT 
CCCAATATTC TGAAACATGT GGTTGGACAG TTCAGTAAGG TGCTGCCGCA TGATAGCAAA 
GCTCGACGAC TTTTTGTAAC AAGTGGTGGC CTTAAAAAAG TTCAAGAGAT AAAAGCAGAA 
CCTGGTTCTC TCCTTCAAGA ATACATCAAC AGTATTAACA GTTGTTACCC CGAGGAAATA 
GTGAGGTATT ATTCCCCTGG ATATTCAGAT ACACTTCTGC AGAGGGTGGA CAGCTATCAA 
CCACTTAATA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29215
Clone ID Related Accession (Same CDS sequence) NM_001253854.2 , NM_001253854.1
Accession Version NM_001253854.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-10-12
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB057157.1, DB025758.1, AK302194.1, AF079363.1 and BC030585.2. Transcript Variant: This variant (3) contains several alternate exons in the 5' end that cause a frameshift and use of a downstream translation start site. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2161674 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCATTCAC TCACCATGGA TCTCTGGATA CCTAATGGAC AGGCAGGTGT AATGTCTTTG 
CTGAGAACTC TTCTTCTGGA CGTGGTCCCA ACAATTCAAC AGACTGCTGC TTTGGCTCTT 
GGGAGACTGG CCAATTATAA TGATGACCTA GCAGAAGCTG TTGTGAAGTG CGACATTCTT 
CCACAGCTTG TTTATTCATT GGCAGAACAG AATCGCTTCT ACAAGAAAGC AGCTGCCTTT 
GTGTTACGAG CAGTTGGTAA ACATTCTCCC CAGCTAGCTC AGGCAATAGT CGATTGTGGA 
GCACTGGATA CGCTGGTCAT ATGCTTGGAA GATTTTGACC CTGGAGTCAA GGAGGCTGCA 
GCCTGGGCAC TTAGATATAT TGCAAGACAT AATGCAGAAC TGTCACAAGC TGTGGTGGAT 
GCAGGAGCTG TTCCTCTTTT AGTACTCTGT ATCCAGGAGC CAGAAATTGC TTTGAAAAGG 
ATTGCTGCTT CGGCCCTCAG TGATATTGCA AAGCATTCTC CAGAGTTAGC ACAGACAGTA 
GTGGATGCAG GAGCTGTTGC TCATTTAGCC CAGATGATCC TGAACCCTGA TGCTAAATTG 
AAGCATCAGA TCCTTTCAGC TCTCAGTCAG GTTTCAAAAC ATTCCGTGGA TCTGGCAGAA 
ATGGTTGTTG AAGCAGAGAT TTTTCCAGTT GTACTTACCT GTCTGAAGGA CAAGGATGAA 
TACGTGAAGA AAAATGCTTC TACTTTAATT AGAGAGATTG CAAAACATAC ACCCGAGCTT 
TCACAGCTGG TAGTTAACGC AGGAGGGGTT GCTGCCGTGA TTGACTGCAT TGGGTCCTGC 
AAAGGGAACA CACGGCTGCC TGGCATCATG ATGCTTGGTT ATGTAGCAGC TCATTCTGAG 
AACCTAGCAA TGGCAGTCAT CATTTCTAAG GGTGTACCCC AGTTGTCAGT CTGCTTGTCA 
GAAGAACCGG AAGATCATAT TAAGGCTGCA GCTGCTTGGG CCTTAGGACA GATTGGAAGA 
CACACTCCTG AACACGCACG GGCTGTTGCA GTCACAAATA CTTTGCCAGT TCTGCTTTCT 
TTGTACATGT CAACAGAAAG TTCTGAGGAT CTCCAAGTAA AAAGTAAAAA AGCCATAAAG 
AATATCCTGC AAAAATGTAC CTACTTACCA GCCCTTGAAC CATTTCTATA TGATGCTCCT 
CCCAATATTC TGAAACATGT GGTTGGACAG TTCAGTAAGG TGCTGCCGCA TGATAGCAAA 
GCTCGACGAC TTTTTGTAAC AAGTGGTGGC CTTAAAAAAG TTCAAGAGAT AAAAGCAGAA 
CCTGGTTCTC TCCTTCAAGA ATACATCAAC AGTATTAACA GTTGTTACCC CGAGGAAATA 
GTGAGGTATT ATTCCCCTGG ATATTCAGAT ACACTTCTGC AGAGGGTGGA CAGCTATCAA 
CCACTTAATA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001240783.1
CDS598..2052
Translation

Target ORF information:

RefSeq Version NM_001253854.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001253854.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCATTCAC TCACCATGGA TCTCTGGATA CCTAATGGAC AGGCAGGTGT AATGTCTTTG 
CTGAGAACTC TTCTTCTGGA CGTGGTCCCA ACAATTCAAC AGACTGCTGC TTTGGCTCTT 
GGGAGACTGG CCAATTATAA TGATGACCTA GCAGAAGCTG TTGTGAAGTG CGACATTCTT 
CCACAGCTTG TTTATTCATT GGCAGAACAG AATCGCTTCT ACAAGAAAGC AGCTGCCTTT 
GTGTTACGAG CAGTTGGTAA ACATTCTCCC CAGCTAGCTC AGGCAATAGT CGATTGTGGA 
GCACTGGATA CGCTGGTCAT ATGCTTGGAA GATTTTGACC CTGGAGTCAA GGAGGCTGCA 
GCCTGGGCAC TTAGATATAT TGCAAGACAT AATGCAGAAC TGTCACAAGC TGTGGTGGAT 
GCAGGAGCTG TTCCTCTTTT AGTACTCTGT ATCCAGGAGC CAGAAATTGC TTTGAAAAGG 
ATTGCTGCTT CGGCCCTCAG TGATATTGCA AAGCATTCTC CAGAGTTAGC ACAGACAGTA 
GTGGATGCAG GAGCTGTTGC TCATTTAGCC CAGATGATCC TGAACCCTGA TGCTAAATTG 
AAGCATCAGA TCCTTTCAGC TCTCAGTCAG GTTTCAAAAC ATTCCGTGGA TCTGGCAGAA 
ATGGTTGTTG AAGCAGAGAT TTTTCCAGTT GTACTTACCT GTCTGAAGGA CAAGGATGAA 
TACGTGAAGA AAAATGCTTC TACTTTAATT AGAGAGATTG CAAAACATAC ACCCGAGCTT 
TCACAGCTGG TAGTTAACGC AGGAGGGGTT GCTGCCGTGA TTGACTGCAT TGGGTCCTGC 
AAAGGGAACA CACGGCTGCC TGGCATCATG ATGCTTGGTT ATGTAGCAGC TCATTCTGAG 
AACCTAGCAA TGGCAGTCAT CATTTCTAAG GGTGTACCCC AGTTGTCAGT CTGCTTGTCA 
GAAGAACCGG AAGATCATAT TAAGGCTGCA GCTGCTTGGG CCTTAGGACA GATTGGAAGA 
CACACTCCTG AACACGCACG GGCTGTTGCA GTCACAAATA CTTTGCCAGT TCTGCTTTCT 
TTGTACATGT CAACAGAAAG TTCTGAGGAT CTCCAAGTAA AAAGTAAAAA AGCCATAAAG 
AATATCCTGC AAAAATGTAC CTACTTACCA GCCCTTGAAC CATTTCTATA TGATGCTCCT 
CCCAATATTC TGAAACATGT GGTTGGACAG TTCAGTAAGG TGCTGCCGCA TGATAGCAAA 
GCTCGACGAC TTTTTGTAAC AAGTGGTGGC CTTAAAAAAG TTCAAGAGAT AAAAGCAGAA 
CCTGGTTCTC TCCTTCAAGA ATACATCAAC AGTATTAACA GTTGTTACCC CGAGGAAATA 
GTGAGGTATT ATTCCCCTGG ATATTCAGAT ACACTTCTGC AGAGGGTGGA CAGCTATCAA 
CCACTTAATA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29216
Clone ID Related Accession (Same CDS sequence) NM_012443.3 , NM_012443.4
Accession Version NM_012443.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB057157.1, DB025758.1, AF079363.1 and BC030585.2. On Dec 8, 2011 this sequence version replaced NM_012443.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL080136.1, AF079363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG GTATTATTCC CCTGGATATT CAGATACACT TCTGCAGAGG 
GTGGACAGCT ATCAACCACT TAATAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036575.1
CDS168..1697
Translation

Target ORF information:

RefSeq Version NM_012443.3
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_012443.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG GTATTATTCC CCTGGATATT CAGATACACT TCTGCAGAGG 
GTGGACAGCT ATCAACCACT TAATAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29216
Clone ID Related Accession (Same CDS sequence) NM_012443.3 , NM_012443.4
Accession Version NM_012443.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-23
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025758.1, AF079363.1 and BC030585.2. On Nov 22, 2018 this sequence version replaced NM_012443.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL080136.1, AF079363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376624.8/ ENSP00000365811.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG GTATTATTCC CCTGGATATT CAGATACACT TCTGCAGAGG 
GTGGACAGCT ATCAACCACT TAATAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036575.1
CDS117..1646
Translation

Target ORF information:

RefSeq Version NM_012443.4
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_012443.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG GTATTATTCC CCTGGATATT CAGATACACT TCTGCAGAGG 
GTGGACAGCT ATCAACCACT TAATAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43907
Clone ID Related Accession (Same CDS sequence) XM_005252645.1 , XM_005252645.2
Accession Version XM_005252645.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG TAAATTCTTT CCAGAGTTGC AAGTTTGTGC ACTTTTTAGG 
AAGTTTATGA GAACTCCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252702.1
CDS197..1717
Translation

Target ORF information:

RefSeq Version XM_005252645.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005252645.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG TAAATTCTTT CCAGAGTTGC AAGTTTGTGC ACTTTTTAGG 
AAGTTTATGA GAACTCCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43907
Clone ID Related Accession (Same CDS sequence) XM_005252645.1 , XM_005252645.2
Accession Version XM_005252645.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005252645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG TAAATTCTTT CCAGAGTTGC AAGTTTGTGC ACTTTTTAGG 
AAGTTTATGA GAACTCCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252702.1
CDS152..1672
Translation

Target ORF information:

RefSeq Version XM_005252645.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005252645.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCA TCAGATCCTT TCAGCTCTCA GTCAGGTTTC AAAACATTCC 
GTGGATCTGG CAGAAATGGT TGTTGAAGCA GAGATTTTTC CAGTTGTACT TACCTGTCTG 
AAGGACAAGG ATGAATACGT GAAGAAAAAT GCTTCTACTT TAATTAGAGA GATTGCAAAA 
CATACACCCG AGCTTTCACA GCTGGTAGTT AACGCAGGAG GGGTTGCTGC CGTGATTGAC 
TGCATTGGGT CCTGCAAAGG GAACACACGG CTGCCTGGCA TCATGATGCT TGGTTATGTA 
GCAGCTCATT CTGAGAACCT AGCAATGGCA GTCATCATTT CTAAGGGTGT ACCCCAGTTG 
TCAGTCTGCT TGTCAGAAGA ACCGGAAGAT CATATTAAGG CTGCAGCTGC TTGGGCCTTA 
GGACAGATTG GAAGACACAC TCCTGAACAC GCACGGGCTG TTGCAGTCAC AAATACTTTG 
CCAGTTCTGC TTTCTTTGTA CATGTCAACA GAAAGTTCTG AGGATCTCCA AGTAAAAAGT 
AAAAAAGCCA TAAAGAATAT CCTGCAAAAA TGTACCTACT TACCAGCCCT TGAACCATTT 
CTATATGATG CTCCTCCCAA TATTCTGAAA CATGTGGTTG GACAGTTCAG TAAGGTGCTG 
CCGCATGATA GCAAAGCTCG ACGACTTTTT GTAACAAGTG GTGGCCTTAA AAAAGTTCAA 
GAGATAAAAG CAGAACCTGG TTCTCTCCTT CAAGAATACA TCAACAGTAT TAACAGTTGT 
TACCCCGAGG AAATAGTGAG TAAATTCTTT CCAGAGTTGC AAGTTTGTGC ACTTTTTAGG 
AAGTTTATGA GAACTCCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43908
Clone ID Related Accession (Same CDS sequence) XM_005252646.1 , XM_005252646.2
Accession Version XM_005252646.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCT TTCACAGCTG GTAGTTAACG CAGGAGGGGT TGCTGCCGTG 
ATTGACTGCA TTGGGTCCTG CAAAGGGAAC ACACGGCTGC CTGGCATCAT GATGCTTGGT 
TATGTAGCAG CTCATTCTGA GAACCTAGCA ATGGCAGTCA TCATTTCTAA GGGTGTACCC 
CAGTTGTCAG TCTGCTTGTC AGAAGAACCG GAAGATCATA TTAAGGCTGC AGCTGCTTGG 
GCCTTAGGAC AGATTGGAAG ACACACTCCT GAACACGCAC GGGCTGTTGC AGTCACAAAT 
ACTTTGCCAG TTCTGCTTTC TTTGTACATG TCAACAGAAA GTTCTGAGGA TCTCCAAGTA 
AAAAGTAAAA AAGCCATAAA GAATATCCTG CAAAAATGTA CCTACTTACC AGCCCTTGAA 
CCATTTCTAT ATGATGCTCC TCCCAATATT CTGAAACATG TGGTTGGACA GTTCAGTAAG 
GTGCTGCCGC ATGATAGCAA AGCTCGACGA CTTTTTGTAA CAAGTGGTGG CCTTAAAAAA 
GTTCAAGAGA TAAAAGCAGA ACCTGGTTCT CTCCTTCAAG AATACATCAA CAGTATTAAC 
AGTTGTTACC CCGAGGAAAT AGTGAGGTAT TATTCCCCTG GATATTCAGA TACACTTCTG 
CAGAGGGTGG ACAGCTATCA ACCACTTAAT AACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252703.1
CDS197..1552
Translation

Target ORF information:

RefSeq Version XM_005252646.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005252646.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCT TTCACAGCTG GTAGTTAACG CAGGAGGGGT TGCTGCCGTG 
ATTGACTGCA TTGGGTCCTG CAAAGGGAAC ACACGGCTGC CTGGCATCAT GATGCTTGGT 
TATGTAGCAG CTCATTCTGA GAACCTAGCA ATGGCAGTCA TCATTTCTAA GGGTGTACCC 
CAGTTGTCAG TCTGCTTGTC AGAAGAACCG GAAGATCATA TTAAGGCTGC AGCTGCTTGG 
GCCTTAGGAC AGATTGGAAG ACACACTCCT GAACACGCAC GGGCTGTTGC AGTCACAAAT 
ACTTTGCCAG TTCTGCTTTC TTTGTACATG TCAACAGAAA GTTCTGAGGA TCTCCAAGTA 
AAAAGTAAAA AAGCCATAAA GAATATCCTG CAAAAATGTA CCTACTTACC AGCCCTTGAA 
CCATTTCTAT ATGATGCTCC TCCCAATATT CTGAAACATG TGGTTGGACA GTTCAGTAAG 
GTGCTGCCGC ATGATAGCAA AGCTCGACGA CTTTTTGTAA CAAGTGGTGG CCTTAAAAAA 
GTTCAAGAGA TAAAAGCAGA ACCTGGTTCT CTCCTTCAAG AATACATCAA CAGTATTAAC 
AGTTGTTACC CCGAGGAAAT AGTGAGGTAT TATTCCCCTG GATATTCAGA TACACTTCTG 
CAGAGGGTGG ACAGCTATCA ACCACTTAAT AACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43908
Clone ID Related Accession (Same CDS sequence) XM_005252646.1 , XM_005252646.2
Accession Version XM_005252646.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product sperm-associated antigen 6 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005252646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCT TTCACAGCTG GTAGTTAACG CAGGAGGGGT TGCTGCCGTG 
ATTGACTGCA TTGGGTCCTG CAAAGGGAAC ACACGGCTGC CTGGCATCAT GATGCTTGGT 
TATGTAGCAG CTCATTCTGA GAACCTAGCA ATGGCAGTCA TCATTTCTAA GGGTGTACCC 
CAGTTGTCAG TCTGCTTGTC AGAAGAACCG GAAGATCATA TTAAGGCTGC AGCTGCTTGG 
GCCTTAGGAC AGATTGGAAG ACACACTCCT GAACACGCAC GGGCTGTTGC AGTCACAAAT 
ACTTTGCCAG TTCTGCTTTC TTTGTACATG TCAACAGAAA GTTCTGAGGA TCTCCAAGTA 
AAAAGTAAAA AAGCCATAAA GAATATCCTG CAAAAATGTA CCTACTTACC AGCCCTTGAA 
CCATTTCTAT ATGATGCTCC TCCCAATATT CTGAAACATG TGGTTGGACA GTTCAGTAAG 
GTGCTGCCGC ATGATAGCAA AGCTCGACGA CTTTTTGTAA CAAGTGGTGG CCTTAAAAAA 
GTTCAAGAGA TAAAAGCAGA ACCTGGTTCT CTCCTTCAAG AATACATCAA CAGTATTAAC 
AGTTGTTACC CCGAGGAAAT AGTGAGGTAT TATTCCCCTG GATATTCAGA TACACTTCTG 
CAGAGGGTGG ACAGCTATCA ACCACTTAAT AACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252703.1
CDS152..1507
Translation

Target ORF information:

RefSeq Version XM_005252646.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 6 (SPAG6), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005252646.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTCAGA GGCAGGTGCT GCAAGTGTTC GAGCAATACC AGAAGGCCAG GACCCAGTTC 
GTGCAGATGG TGGCGGAGCT GGCGACTAGA CCCCAAAACA TCGAGACGCT GCAGAACGCG 
GGTGTAATGT CTTTGCTGAG AACTCTTCTT CTGGACGTGG TCCCAACAAT TCAACAGACT 
GCTGCTTTGG CTCTTGGGAG ACTGGCCAAT TATAATGATG ACCTAGCAGA AGCTGTTGTG 
AAGTGCGACA TTCTTCCACA GCTTGTTTAT TCATTGGCAG AACAGAATCG CTTCTACAAG 
AAAGCAGCTG CCTTTGTGTT ACGAGCAGTT GGTAAACATT CTCCCCAGCT AGCTCAGGCA 
ATAGTCGATT GTGGAGCACT GGATACGCTG GTCATATGCT TGGAAGATTT TGACCCTGGA 
GTCAAGGAGG CTGCAGCCTG GGCACTTAGA TATATTGCAA GACATAATGC AGAACTGTCA 
CAAGCTGTGG TGGATGCAGG AGCTGTTCCT CTTTTAGTAC TCTGTATCCA GGAGCCAGAA 
ATTGCTTTGA AAAGGATTGC TGCTTCGGCC CTCAGTGATA TTGCAAAGCA TTCTCCAGAG 
TTAGCACAGA CAGTAGTGGA TGCAGGAGCT GTTGCTCATT TAGCCCAGAT GATCCTGAAC 
CCTGATGCTA AATTGAAGCT TTCACAGCTG GTAGTTAACG CAGGAGGGGT TGCTGCCGTG 
ATTGACTGCA TTGGGTCCTG CAAAGGGAAC ACACGGCTGC CTGGCATCAT GATGCTTGGT 
TATGTAGCAG CTCATTCTGA GAACCTAGCA ATGGCAGTCA TCATTTCTAA GGGTGTACCC 
CAGTTGTCAG TCTGCTTGTC AGAAGAACCG GAAGATCATA TTAAGGCTGC AGCTGCTTGG 
GCCTTAGGAC AGATTGGAAG ACACACTCCT GAACACGCAC GGGCTGTTGC AGTCACAAAT 
ACTTTGCCAG TTCTGCTTTC TTTGTACATG TCAACAGAAA GTTCTGAGGA TCTCCAAGTA 
AAAAGTAAAA AAGCCATAAA GAATATCCTG CAAAAATGTA CCTACTTACC AGCCCTTGAA 
CCATTTCTAT ATGATGCTCC TCCCAATATT CTGAAACATG TGGTTGGACA GTTCAGTAAG 
GTGCTGCCGC ATGATAGCAA AGCTCGACGA CTTTTTGTAA CAAGTGGTGG CCTTAAAAAA 
GTTCAAGAGA TAAAAGCAGA ACCTGGTTCT CTCCTTCAAG AATACATCAA CAGTATTAAC 
AGTTGTTACC CCGAGGAAAT AGTGAGGTAT TATTCCCCTG GATATTCAGA TACACTTCTG 
CAGAGGGTGG ACAGCTATCA ACCACTTAAT AACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes.
Oncology reports37(5)2839-2846(2017 May)
Li X,Yang B,Wang L,Chen L,Luo X,Liu L


book

SPAG6 and L1TD1 are transcriptionally regulated by DNA methylation in non-small cell lung cancers.
Molecular cancer16(1)1(2017 01)
Altenberger C,Heller G,Ziegler B,Tomasich E,Marhold M,Topakian T,M?llauer L,Heffeter P,Lang G,End-Pf?tzenreuter A,D?me B,Arns BM,Klepetko W,Zielinski CC,Z?chbauer-M?ller S


book

SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis.
International journal of oncology46(2)649-56(2015 Feb)
Yang B,Wang L,Luo X,Chen L,Yang Z,Liu L