EIF5B cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | EIF5B |
| Entrez Gene ID | 9669 |
| Full Name | eukaryotic translation initiation factor 5B |
| Synonyms | IF2 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_015904.4 | NP_056988.3 | eukaryotic translation initiation factor 5B |
| NM_015904.3 | NP_056988.3 | eukaryotic translation initiation factor 5B |
| Homo sapiens (human) | EIF5B | NP_056988.3 |
| Rattus norvegicus (Norway rat) | Eif5b | NP_001103611.1 |
| Bos taurus (cattle) | EIF5B | XP_003586655.1 |
| Gallus gallus (chicken) | EIF5B | NP_001033585.1 |
| Pan troglodytes (chimpanzee) | EIF5B | XP_001159759.1 |
| Canis lupus familiaris (dog) | EIF5B | XP_005626079.1 |
| EIF5B | XP_001103720.2 | |
| Xenopus tropicalis (tropical clawed frog) | eif5b | XP_004911863.1 |
| Mus musculus (house mouse) | Eif5b | NP_938045.2 |
| Danio rerio (zebrafish) | eif5b | XP_001923573.4 |
Related articles in PubMed
Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
Ho JJD, Balukoff NC, Cervantes G, Malcolm PD, Krieger JR, Lee S
Cell reports22(1)17-26(2018 Jan)
eIF5B increases ASAP1 expression to promote HCC proliferation and invasion.
Wang ZG, Zheng H, Gao W, Han J, Cao JZ, Yang Y, Li S, Gao R, Liu H, Pan ZY, Fu SY, Gu FM, Xing H, Ni JS, Yan HL, Ren H, Zhou WP
Oncotarget7(38)62327-62339(2016 09)
eIF1A/eIF5B interaction network and its functions in translation initiation complex assembly and remodeling.
Nag N, Lin KY, Edmonds KA, Yu J, Nadkarni D, Marintcheva B, Marintchev A
Nucleic acids research44(15)7441-56(2016 Sep)
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Jiang X, Jiang X, Feng Y, Xu R, Wang Q, Deng H
PloS one11(12)e0168387(2016)
Upregulation of eIF5B controls cell-cycle arrest and specific developmental stages.
Lee S, Truesdell SS, Bukhari SI, Lee JH, LeTonqueze O, Vasudevan S
Proceedings of the National Academy of Sciences of the United States of America111(41)E4315-22(2014 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
results indicate that the interactions between eIF1A and eIF5B are being continuously rearranged during translation initiation; presentation of a model how the dynamic eIF1A/eIF5B interaction network can promote remodeling of the translation initiation complexes, and the roles in the process played by intrinsically disordered protein segments
Title: eIF1A/eIF5B interaction network and its functions in translation initiation complex assembly and remodeling.
eIF5B silencing provides a negative feedback to deactivate MAPK signaling
Title: Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
eIF5B overexpression promotes maturation of G0-like immature oocytes and causes cell death, an alternative to G0, in serum-starved THP1 cells.
Title: Upregulation of eIF5B controls cell-cycle arrest and specific developmental stages.
The authors show that the cleavage of initiation factor eIF5B during enteroviral infection, along with the viral internal ribosome entry site, plays a role in mediating viral translation under conditions that are nonpermissive for host cell translation.
Title: Poliovirus switches to an eIF2-independent mode of translation during infection.
3Cpro-mediated cleavage of eIF5B may thus play an accessory role in the shutoff of translation that occurs in enterovirus-infected cells.
Title: Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases.
The following EIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu30523 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_015904.4 , NM_015904.3 | |
| Accession Version | NM_015904.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-06-07 | |
| Organism | Homo sapiens(human) | |
| Product | eukaryotic translation initiation factor 5B | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX485839.1, AB018284.1, BC032639.1 and AC018690.5. On Nov 22, 2018 this sequence version replaced NM_015904.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018284.1, AJ006776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000289371.11/ ENSP00000289371.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_056988.3 |
| CDS | 167..3829 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_015904.4 |
ORF Insert Sequence:
1 | ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu30523 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_015904.4 , NM_015904.3 | |
| Accession Version | NM_015904.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-30 | |
| Organism | Homo sapiens(human) | |
| Product | eukaryotic translation initiation factor 5B | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX485839.1, AB018284.1, BC032639.1 and AC018690.5. On Dec 29, 2005 this sequence version replaced NM_015904.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018284.1, AJ006776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. | |
1 | ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_056988.3 |
| CDS | 185..3847 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_015904.3 |
ORF Insert Sequence:
1 | ATGGGGAAGA AACAGAAAAA CAAGAGCGAA GACAGCACCA AGGATGACAT TGATCTTGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. |
|
eIF5B increases ASAP1 expression to promote HCC proliferation and invasion. |
|
eIF1A/eIF5B interaction network and its functions in translation initiation complex assembly and remodeling. |
|
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis. |
|
Upregulation of eIF5B controls cell-cycle arrest and specific developmental stages. |