This gene encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015].
The following MLEC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLEC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718506.1, BC016297.2, AC069234.35 and AA831396.1. On Jan 15, 2015 this sequence version replaced NM_014730.2.
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
##Evidence-Data-START##
Transcript exon combination :: SRR1803612.133333.1, SRR1803617.58579.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718506.1, BC016297.2, AC069234.35 and AA831396.1. On Nov 23, 2018 this sequence version replaced NM_014730.3.
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
##Evidence-Data-START##
Transcript exon combination :: SRR1803617.58579.1, SRR1660805.33469.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000228506.8/ ENSP00000228506.3
RefSeq Select criteria :: based on conservation, expression, longest protein
##RefSeq-Attributes-END##
COMPLETENESS: full length.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069234.35, BI458950.1 and AA831396.1.
Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and coding region which results in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
##Evidence-Data-START##
Transcript exon combination :: BI458950.1 [ECO:0000332]
##Evidence-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205
Annotation Version :: 109.20191205
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.3
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205
Annotation Version :: 109.20191205
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.3
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718506.1, BE326824.1, AC069234.35 and AA831396.1.
Transcript Variant: This variant (3) lacks two consecutive exon in the central coding region which results in a frameshift compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus compare to isoform 1.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
##Evidence-Data-START##
Transcript exon combination :: BE326824.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718506.1, BE326824.1, AC069234.35 and AA831396.1. On May 31, 2019 this sequence version replaced NM_001303628.1.
Transcript Variant: This variant (3) lacks two consecutive exon in the central coding region which results in a frameshift compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus compare to isoform 1.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
##Evidence-Data-START##
Transcript exon combination :: BE326824.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
User Manual
NM_014730.3
