Micalcl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Micalcl
Entrez Gene ID	100504195
Full Name	MICAL C-terminal like
Synonyms	4921517J23Rik
General protein information	Preferred Names MICAL C-terminal like Names MICAL C-terminal-like protein ERK2-binding testicular protein 1 MICAL C-terminal-like protein-like ebitein-1 ebitein1 mical-cL
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F1

mRNA and Protein(s)

mRNA Protein Name

XM_030241932.1 XP_030097792.1 MICAL C-terminal-like protein isoform X1

NM_027587.2 NP_081863.2 MICAL C-terminal-like protein
Pathways from BioSystems

mRNA	Protein	Name
XM_030241932.1	XP_030097792.1	MICAL C-terminal-like protein isoform X1
NM_027587.2	NP_081863.2	MICAL C-terminal-like protein

Homologies

Pan troglodytes (chimpanzee)	MICALCL	XP_508290.2
Bos taurus (cattle)	MICALCL	XP_003587005.2
Mus musculus (house mouse)	Micalcl	NP_081863.2
	MICALCL	XP_001096072.1
Rattus norvegicus (Norway rat)	Micalcl	NP_872610.2
Homo sapiens (human)	MICALCL	NP_116256.2

Gene Ontology
Bibliography

Related articles in PubMed

ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2.
Miura K
Biochemical and biophysical research communications375(3)367-71(2008 Oct)

Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.
Miura K, Imaki J
Biochimica et biophysica acta1784(9)1319-25(2008 Sep)

Molecular cloning of Ebitein1: a novel extracellular signal-regulated kinase 2-binding protein in testis.
Miura K, Imaki J
Biochemical and biophysical research communications368(2)336-42(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EBITEIN1 is a phosphoprotein and a downstream interactor of ERK2 that participates in the intracellular signal transduction pathway mediating the morphogenetic development of round spermatids into spermatozoa.
Title: ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2.

Based on empirical and bioinformatic analyses, we propose two domains in EBITEIN1. One is the minimal sequence for binding ERK2 (EB domain) and the other is the EBITEIN1 C-terminal domain (ECT domain).
Title: Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.

The following Micalcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Micalcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04389	NM_027587.2 Latest version!	Mus musculus MICAL C-terminal like (Micalcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$440.30 $629.00
OMu04389	XM_030241932.1 Latest version!	Mus musculus MICAL C-terminal like (Micalcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Micalcl ( NM_027587.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081863.2 Mus musculus MICAL C-terminal like (Micalcl), mRNA.

Price & Availability↑

CloneID	OMu04389
Clone ID Related Accession (Same CDS sequence)	XM_030241932.1 , NM_027587.2
Accession Version	NM_027587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	MICAL C-terminal-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132392.3. On or before Jan 14, 2011 this sequence version replaced XM_003084725.1, NM_027587.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB359922.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCAAA GAGCACCATC GCCTCCAAAG GAGCCGCCTC CTCCTCCATC TTTGTCTTCT 
TCATCTTCCC TACCATCTTC ATTTTCTTCA GCCAGTGTAC CTGGGCACAC TGCAGACGAT 
TCCTCCTCTC CCCAGGTGCC AGCTTACAAC CTCCACTCCC CACAGATCTC CAGAGGCGAT 
GTCAGCCCCA CTCCAATCTA CCTCAGACGT GCCAGGGCTC AAGGGATTGT GAAGGAAATT 
CCCCTCTACC TGCCGCATAG CCCCATGCTA GAGAGCACAG AAGACTGCCT GGTCGAGCCC 
GGTAGAGAAA GCCTCAGAAG TCCTGAGGAG ATCTCCTCTT CAGAGGGCTG CCAGGAAGCC 
CGGGCTCTTG GAAACACCAG AAGCATACAG CACCCCATTT TAGGGAAGGA CCAGTATTTG 
CCCAATCAAA ACCTAGCTCT TGGTGCTGCA GGGAACCCTG GAGACCCGAG GGAGGAATCC 
AGGATGGGAC AACCAGGTGG ACCAGAGCTG TCCAAAGAAA GGAAGTTGGG CTTGAAGAAA 
TTAGTCCTCA CTGAGGAGCA GAAGAACAAG TTGCTAGACT GGAGTGACTG CACCCAGGAG 
CACAAGACTG GGGAGCAGCT CTCTCAGGAA AGTGCTGAGA ACATCAGAGG TGGTAGCCTG 
AAGCCAACCT GCTCTTCTAC CCTCTCTCAG GCGGTGAAAG AGAAGCTGCT ATCCCAGAAA 
AAGGCCCTGG GGGGGATGCG TACCCCAGCT GTCAAAGCAC CTCAGGAGCG AGAGGTGCCC 
CCACCCAAGT CCCCCCTGAA GCTCATAGCA AATGCCATCC TTAGGTCTCT GCTCCACAAC 
TCCGAAGCTG GAAAGAAAAC CAGCCCTAAG CCAGAGTCCA AAACTTTGCC TAGGGGCCAG 
CCACATGCTC GTTCGTTCAG CCTCCGGAAA CTGGGTTCCA GTAAAGATGG GGACCAGCAG 
TCTCCAGGGA GACACATGGC CAAGAAGGCT TCAGCATTCT TCTCTCTGGC TTCTCCAACA 
TCTAAGGTTG CCCAAGCCTC AGACCTTAGC CTTCCCAACT CCATCCTCCG CAGCCGGAGT 
TTACCGAGTC GTCCATCCAA GATGTTCTTT TCAACCACAC CTCACAGCAA GGTTGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGATGCCA CTCACAGTCC AAAGACAGGA 
GCCTCACATA TTTCTTCCCT TGGCCTCAAA GATAAATCCT TTGAGAGTTT TCTCCAGGAA 
TGCAAACAAA GAAAGGACAT TGGGGACTTC TTTAATAGCC CCAAGGAGGA GGGGCCACCA 
GGGAACAGAG TCCCATCCTT GGAGAAGTTG GTACAGCCTG TTGGCAGCAC CTCCATGGGA 
CAGGTGGCAC ATCCTTCTTC CACAGGGCAA GATGCACACC CTGTAGCTCC AGTGACAGAA 
GCCACCTCTT CTCCCACCTC GTCCTCTGCA GAGGAAGAAG CCGACTCCCA GCTTTCCTTA 
AGGATAAAGG AGAAGATACT CAGAAGAAGG AGGAAGCTGG AAAAGCAGTC AGCTAAGCAA 
GAAGAATTGA AAAGACTCCA TAAGGCTCAG GCCATCCAGA GACAACTAGA GGAGGTGGAG 
GAGCGGCAGA GGACATTGGC GATCCAGGGC GTGAAGCTGG AGAAGGTGCT GAGAGGAGAA 
GCAGATTCAG GGACACAGGA TGAAGCCCAG CTTTTGCAGG AATGGTTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCG GAGCTACTGA TCATGGCCCA AGAACTAGAA 
CTAGAAGATC ATCAGAGCAG ACTGGAGCAG AAACTACGAC AGAAAATGCT CAAGGATGAG 
GGCCAGAAAG ACGAGAATGA CCTAAAGGAG GAGCAAGAAA TATTCGAGGA GATGATGCAG 
GTGATTGAGC AAAGAAACAA GCTCGTGGAT TCCCTAGAGG AACAGCGGGT CAAAGAAAGA 
ACCCAAGACC AACACTTTGA AAACTTTGTT CTCTCCAGAG GCTGTCAGCT CAGCAGGACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081863.2
CDS	238..2280
Translation	MNQRAPSPPKEPPPPPSLSSSSSLPSSFSSASVPGHTADDSSSPQVPAYNLHSPQISRGDVSPTPIYLRRARAQGIVKEIPLYLPHSPMLESTEDCLVEPGRESLRSPEEISSSEGCQEARALGNTRSIQHPILGKDQYLPNQNLALGAAGNPGDPREESRMGQPGGPELSKERKLGLKKLVLTEEQKNKLLDWSDCTQEHKTGEQLSQESAENIRGGSLKPTCSSTLSQAVKEKLLSQKKALGGMRTPAVKAPQEREVPPPKSPLKLIANAILRSLLHNSEAGKKTSPKPESKTLPRGQPHARSFSLRKLGSSKDGDQQSPGRHMAKKASAFFSLASPTSKVAQASDLSLPNSILRSRSLPSRPSKMFFSTTPHSKVEDVPTLLEKVSLQDATHSPKTGASHISSLGLKDKSFESFLQECKQRKDIGDFFNSPKEEGPPGNRVPSLEKLVQPVGSTSMGQVAHPSSTGQDAHPVAPVTEATSSPTSSSAEEEADSQLSLRIKEKILRRRRKLEKQSAKQEELKRLHKAQAIQRQLEEVEERQRTLAIQGVKLEKVLRGEADSGTQDEAQLLQEWFKLVLEKNKLMRYESELLIMAQELELEDHQSRLEQKLRQKMLKDEGQKDENDLKEEQEIFEEMMQVIEQRNKLVDSLEEQRVKERTQDQHFENFVLSRGCQLSRT

Target ORF information:

RefSeq Version	NM_027587.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus MICAL C-terminal like (Micalcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027587.2

ORF Insert Sequence:

ATGAACCAAA GAGCACCATC GCCTCCAAAG GAGCCGCCTC CTCCTCCATC TTTGTCTTCT 
TCATCTTCCC TACCATCTTC ATTTTCTTCA GCCAGTGTAC CTGGGCACAC TGCAGACGAT 
TCCTCCTCTC CCCAGGTGCC AGCTTACAAC CTCCACTCCC CACAGATCTC CAGAGGCGAT 
GTCAGCCCCA CTCCAATCTA CCTCAGACGT GCCAGGGCTC AAGGGATTGT GAAGGAAATT 
CCCCTCTACC TGCCGCATAG CCCCATGCTA GAGAGCACAG AAGACTGCCT GGTCGAGCCC 
GGTAGAGAAA GCCTCAGAAG TCCTGAGGAG ATCTCCTCTT CAGAGGGCTG CCAGGAAGCC 
CGGGCTCTTG GAAACACCAG AAGCATACAG CACCCCATTT TAGGGAAGGA CCAGTATTTG 
CCCAATCAAA ACCTAGCTCT TGGTGCTGCA GGGAACCCTG GAGACCCGAG GGAGGAATCC 
AGGATGGGAC AACCAGGTGG ACCAGAGCTG TCCAAAGAAA GGAAGTTGGG CTTGAAGAAA 
TTAGTCCTCA CTGAGGAGCA GAAGAACAAG TTGCTAGACT GGAGTGACTG CACCCAGGAG 
CACAAGACTG GGGAGCAGCT CTCTCAGGAA AGTGCTGAGA ACATCAGAGG TGGTAGCCTG 
AAGCCAACCT GCTCTTCTAC CCTCTCTCAG GCGGTGAAAG AGAAGCTGCT ATCCCAGAAA 
AAGGCCCTGG GGGGGATGCG TACCCCAGCT GTCAAAGCAC CTCAGGAGCG AGAGGTGCCC 
CCACCCAAGT CCCCCCTGAA GCTCATAGCA AATGCCATCC TTAGGTCTCT GCTCCACAAC 
TCCGAAGCTG GAAAGAAAAC CAGCCCTAAG CCAGAGTCCA AAACTTTGCC TAGGGGCCAG 
CCACATGCTC GTTCGTTCAG CCTCCGGAAA CTGGGTTCCA GTAAAGATGG GGACCAGCAG 
TCTCCAGGGA GACACATGGC CAAGAAGGCT TCAGCATTCT TCTCTCTGGC TTCTCCAACA 
TCTAAGGTTG CCCAAGCCTC AGACCTTAGC CTTCCCAACT CCATCCTCCG CAGCCGGAGT 
TTACCGAGTC GTCCATCCAA GATGTTCTTT TCAACCACAC CTCACAGCAA GGTTGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGATGCCA CTCACAGTCC AAAGACAGGA 
GCCTCACATA TTTCTTCCCT TGGCCTCAAA GATAAATCCT TTGAGAGTTT TCTCCAGGAA 
TGCAAACAAA GAAAGGACAT TGGGGACTTC TTTAATAGCC CCAAGGAGGA GGGGCCACCA 
GGGAACAGAG TCCCATCCTT GGAGAAGTTG GTACAGCCTG TTGGCAGCAC CTCCATGGGA 
CAGGTGGCAC ATCCTTCTTC CACAGGGCAA GATGCACACC CTGTAGCTCC AGTGACAGAA 
GCCACCTCTT CTCCCACCTC GTCCTCTGCA GAGGAAGAAG CCGACTCCCA GCTTTCCTTA 
AGGATAAAGG AGAAGATACT CAGAAGAAGG AGGAAGCTGG AAAAGCAGTC AGCTAAGCAA 
GAAGAATTGA AAAGACTCCA TAAGGCTCAG GCCATCCAGA GACAACTAGA GGAGGTGGAG 
GAGCGGCAGA GGACATTGGC GATCCAGGGC GTGAAGCTGG AGAAGGTGCT GAGAGGAGAA 
GCAGATTCAG GGACACAGGA TGAAGCCCAG CTTTTGCAGG AATGGTTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCG GAGCTACTGA TCATGGCCCA AGAACTAGAA 
CTAGAAGATC ATCAGAGCAG ACTGGAGCAG AAACTACGAC AGAAAATGCT CAAGGATGAG 
GGCCAGAAAG ACGAGAATGA CCTAAAGGAG GAGCAAGAAA TATTCGAGGA GATGATGCAG 
GTGATTGAGC AAAGAAACAA GCTCGTGGAT TCCCTAGAGG AACAGCGGGT CAAAGAAAGA 
ACCCAAGACC AACACTTTGA AAACTTTGTT CTCTCCAGAG GCTGTCAGCT CAGCAGGACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Micalcl ( XM_030241932.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097792.1 Mus musculus MICAL C-terminal like (Micalcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04389
Clone ID Related Accession (Same CDS sequence)	XM_030241932.1 , NM_027587.2
Accession Version	XM_030241932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MICAL C-terminal-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAAA GAGCACCATC GCCTCCAAAG GAGCCGCCTC CTCCTCCATC TTTGTCTTCT 
TCATCTTCCC TACCATCTTC ATTTTCTTCA GCCAGTGTAC CTGGGCACAC TGCAGACGAT 
TCCTCCTCTC CCCAGGTGCC AGCTTACAAC CTCCACTCCC CACAGATCTC CAGAGGCGAT 
GTCAGCCCCA CTCCAATCTA CCTCAGACGT GCCAGGGCTC AAGGGATTGT GAAGGAAATT 
CCCCTCTACC TGCCGCATAG CCCCATGCTA GAGAGCACAG AAGACTGCCT GGTCGAGCCC 
GGTAGAGAAA GCCTCAGAAG TCCTGAGGAG ATCTCCTCTT CAGAGGGCTG CCAGGAAGCC 
CGGGCTCTTG GAAACACCAG AAGCATACAG CACCCCATTT TAGGGAAGGA CCAGTATTTG 
CCCAATCAAA ACCTAGCTCT TGGTGCTGCA GGGAACCCTG GAGACCCGAG GGAGGAATCC 
AGGATGGGAC AACCAGGTGG ACCAGAGCTG TCCAAAGAAA GGAAGTTGGG CTTGAAGAAA 
TTAGTCCTCA CTGAGGAGCA GAAGAACAAG TTGCTAGACT GGAGTGACTG CACCCAGGAG 
CACAAGACTG GGGAGCAGCT CTCTCAGGAA AGTGCTGAGA ACATCAGAGG TGGTAGCCTG 
AAGCCAACCT GCTCTTCTAC CCTCTCTCAG GCGGTGAAAG AGAAGCTGCT ATCCCAGAAA 
AAGGCCCTGG GGGGGATGCG TACCCCAGCT GTCAAAGCAC CTCAGGAGCG AGAGGTGCCC 
CCACCCAAGT CCCCCCTGAA GCTCATAGCA AATGCCATCC TTAGGTCTCT GCTCCACAAC 
TCCGAAGCTG GAAAGAAAAC CAGCCCTAAG CCAGAGTCCA AAACTTTGCC TAGGGGCCAG 
CCACATGCTC GTTCGTTCAG CCTCCGGAAA CTGGGTTCCA GTAAAGATGG GGACCAGCAG 
TCTCCAGGGA GACACATGGC CAAGAAGGCT TCAGCATTCT TCTCTCTGGC TTCTCCAACA 
TCTAAGGTTG CCCAAGCCTC AGACCTTAGC CTTCCCAACT CCATCCTCCG CAGCCGGAGT 
TTACCGAGTC GTCCATCCAA GATGTTCTTT TCAACCACAC CTCACAGCAA GGTTGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGATGCCA CTCACAGTCC AAAGACAGGA 
GCCTCACATA TTTCTTCCCT TGGCCTCAAA GATAAATCCT TTGAGAGTTT TCTCCAGGAA 
TGCAAACAAA GAAAGGACAT TGGGGACTTC TTTAATAGCC CCAAGGAGGA GGGGCCACCA 
GGGAACAGAG TCCCATCCTT GGAGAAGTTG GTACAGCCTG TTGGCAGCAC CTCCATGGGA 
CAGGTGGCAC ATCCTTCTTC CACAGGGCAA GATGCACACC CTGTAGCTCC AGTGACAGAA 
GCCACCTCTT CTCCCACCTC GTCCTCTGCA GAGGAAGAAG CCGACTCCCA GCTTTCCTTA 
AGGATAAAGG AGAAGATACT CAGAAGAAGG AGGAAGCTGG AAAAGCAGTC AGCTAAGCAA 
GAAGAATTGA AAAGACTCCA TAAGGCTCAG GCCATCCAGA GACAACTAGA GGAGGTGGAG 
GAGCGGCAGA GGACATTGGC GATCCAGGGC GTGAAGCTGG AGAAGGTGCT GAGAGGAGAA 
GCAGATTCAG GGACACAGGA TGAAGCCCAG CTTTTGCAGG AATGGTTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCG GAGCTACTGA TCATGGCCCA AGAACTAGAA 
CTAGAAGATC ATCAGAGCAG ACTGGAGCAG AAACTACGAC AGAAAATGCT CAAGGATGAG 
GGCCAGAAAG ACGAGAATGA CCTAAAGGAG GAGCAAGAAA TATTCGAGGA GATGATGCAG 
GTGATTGAGC AAAGAAACAA GCTCGTGGAT TCCCTAGAGG AACAGCGGGT CAAAGAAAGA 
ACCCAAGACC AACACTTTGA AAACTTTGTT CTCTCCAGAG GCTGTCAGCT CAGCAGGACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097792.1
CDS	452..2494
Translation	MNQRAPSPPKEPPPPPSLSSSSSLPSSFSSASVPGHTADDSSSPQVPAYNLHSPQISRGDVSPTPIYLRRARAQGIVKEIPLYLPHSPMLESTEDCLVEPGRESLRSPEEISSSEGCQEARALGNTRSIQHPILGKDQYLPNQNLALGAAGNPGDPREESRMGQPGGPELSKERKLGLKKLVLTEEQKNKLLDWSDCTQEHKTGEQLSQESAENIRGGSLKPTCSSTLSQAVKEKLLSQKKALGGMRTPAVKAPQEREVPPPKSPLKLIANAILRSLLHNSEAGKKTSPKPESKTLPRGQPHARSFSLRKLGSSKDGDQQSPGRHMAKKASAFFSLASPTSKVAQASDLSLPNSILRSRSLPSRPSKMFFSTTPHSKVEDVPTLLEKVSLQDATHSPKTGASHISSLGLKDKSFESFLQECKQRKDIGDFFNSPKEEGPPGNRVPSLEKLVQPVGSTSMGQVAHPSSTGQDAHPVAPVTEATSSPTSSSAEEEADSQLSLRIKEKILRRRRKLEKQSAKQEELKRLHKAQAIQRQLEEVEERQRTLAIQGVKLEKVLRGEADSGTQDEAQLLQEWFKLVLEKNKLMRYESELLIMAQELELEDHQSRLEQKLRQKMLKDEGQKDENDLKEEQEIFEEMMQVIEQRNKLVDSLEEQRVKERTQDQHFENFVLSRGCQLSRT

Target ORF information:

RefSeq Version	XM_030241932.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MICAL C-terminal like (Micalcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241932.1

ORF Insert Sequence:

ATGAACCAAA GAGCACCATC GCCTCCAAAG GAGCCGCCTC CTCCTCCATC TTTGTCTTCT 
TCATCTTCCC TACCATCTTC ATTTTCTTCA GCCAGTGTAC CTGGGCACAC TGCAGACGAT 
TCCTCCTCTC CCCAGGTGCC AGCTTACAAC CTCCACTCCC CACAGATCTC CAGAGGCGAT 
GTCAGCCCCA CTCCAATCTA CCTCAGACGT GCCAGGGCTC AAGGGATTGT GAAGGAAATT 
CCCCTCTACC TGCCGCATAG CCCCATGCTA GAGAGCACAG AAGACTGCCT GGTCGAGCCC 
GGTAGAGAAA GCCTCAGAAG TCCTGAGGAG ATCTCCTCTT CAGAGGGCTG CCAGGAAGCC 
CGGGCTCTTG GAAACACCAG AAGCATACAG CACCCCATTT TAGGGAAGGA CCAGTATTTG 
CCCAATCAAA ACCTAGCTCT TGGTGCTGCA GGGAACCCTG GAGACCCGAG GGAGGAATCC 
AGGATGGGAC AACCAGGTGG ACCAGAGCTG TCCAAAGAAA GGAAGTTGGG CTTGAAGAAA 
TTAGTCCTCA CTGAGGAGCA GAAGAACAAG TTGCTAGACT GGAGTGACTG CACCCAGGAG 
CACAAGACTG GGGAGCAGCT CTCTCAGGAA AGTGCTGAGA ACATCAGAGG TGGTAGCCTG 
AAGCCAACCT GCTCTTCTAC CCTCTCTCAG GCGGTGAAAG AGAAGCTGCT ATCCCAGAAA 
AAGGCCCTGG GGGGGATGCG TACCCCAGCT GTCAAAGCAC CTCAGGAGCG AGAGGTGCCC 
CCACCCAAGT CCCCCCTGAA GCTCATAGCA AATGCCATCC TTAGGTCTCT GCTCCACAAC 
TCCGAAGCTG GAAAGAAAAC CAGCCCTAAG CCAGAGTCCA AAACTTTGCC TAGGGGCCAG 
CCACATGCTC GTTCGTTCAG CCTCCGGAAA CTGGGTTCCA GTAAAGATGG GGACCAGCAG 
TCTCCAGGGA GACACATGGC CAAGAAGGCT TCAGCATTCT TCTCTCTGGC TTCTCCAACA 
TCTAAGGTTG CCCAAGCCTC AGACCTTAGC CTTCCCAACT CCATCCTCCG CAGCCGGAGT 
TTACCGAGTC GTCCATCCAA GATGTTCTTT TCAACCACAC CTCACAGCAA GGTTGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGATGCCA CTCACAGTCC AAAGACAGGA 
GCCTCACATA TTTCTTCCCT TGGCCTCAAA GATAAATCCT TTGAGAGTTT TCTCCAGGAA 
TGCAAACAAA GAAAGGACAT TGGGGACTTC TTTAATAGCC CCAAGGAGGA GGGGCCACCA 
GGGAACAGAG TCCCATCCTT GGAGAAGTTG GTACAGCCTG TTGGCAGCAC CTCCATGGGA 
CAGGTGGCAC ATCCTTCTTC CACAGGGCAA GATGCACACC CTGTAGCTCC AGTGACAGAA 
GCCACCTCTT CTCCCACCTC GTCCTCTGCA GAGGAAGAAG CCGACTCCCA GCTTTCCTTA 
AGGATAAAGG AGAAGATACT CAGAAGAAGG AGGAAGCTGG AAAAGCAGTC AGCTAAGCAA 
GAAGAATTGA AAAGACTCCA TAAGGCTCAG GCCATCCAGA GACAACTAGA GGAGGTGGAG 
GAGCGGCAGA GGACATTGGC GATCCAGGGC GTGAAGCTGG AGAAGGTGCT GAGAGGAGAA 
GCAGATTCAG GGACACAGGA TGAAGCCCAG CTTTTGCAGG AATGGTTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCG GAGCTACTGA TCATGGCCCA AGAACTAGAA 
CTAGAAGATC ATCAGAGCAG ACTGGAGCAG AAACTACGAC AGAAAATGCT CAAGGATGAG 
GGCCAGAAAG ACGAGAATGA CCTAAAGGAG GAGCAAGAAA TATTCGAGGA GATGATGCAG 
GTGATTGAGC AAAGAAACAA GCTCGTGGAT TCCCTAGAGG AACAGCGGGT CAAAGAAAGA 
ACCCAAGACC AACACTTTGA AAACTTTGTT CTCTCCAGAG GCTGTCAGCT CAGCAGGACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2.
Biochemical and biophysical research communications375(3)367-71(2008 Oct)
Miura K

Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.
Biochimica et biophysica acta1784(9)1319-25(2008 Sep)
Miura K,Imaki J

Molecular cloning of Ebitein1: a novel extracellular signal-regulated kinase 2-binding protein in testis.
Biochemical and biophysical research communications368(2)336-42(2008 Apr)
Miura K,Imaki J

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Micalcl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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