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Spred3 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Spred3
Entrez Gene ID 101809
Full Name sprouty-related, EVH1 domain containing 3
Synonyms AW047143,D130060H24Rik,Spred-3
General protein information
Preferred Names

sprouty-related, EVH1 domain containing 3

Names

sprouty-related, EVH1 domain-containing protein 3
Sprouty-related EVH1 domain-containing protein
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

7

7|7 B1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006539451.1 XP_006539514.1 sprouty-related, EVH1 domain-containing protein 3 isoform X1
XM_006539455.3 XP_006539518.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_006539453.3 XP_006539516.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_006539452.1 XP_006539515.1 sprouty-related, EVH1 domain-containing protein 3 isoform X2
XM_006539454.3 XP_006539517.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_017321915.1 XP_017177404.1 sprouty-related, EVH1 domain-containing protein 3 isoform X6
NM_182927.3 NP_891557.3 sprouty-related, EVH1 domain-containing protein 3
XM_017321914.1 XP_017177403.1 sprouty-related, EVH1 domain-containing protein 3 isoform X5
XM_006539452.2 XP_006539515.1 sprouty-related, EVH1 domain-containing protein 3 isoform X2
XM_006539454.4 XP_006539517.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_030241962.1 XP_030097822.1 sprouty-related, EVH1 domain-containing protein 3 isoform X4
XM_017321915.2 XP_017177404.1 sprouty-related, EVH1 domain-containing protein 3 isoform X5
XM_006539456.2 XP_006539519.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_011250410.2 XP_011248712.2 sprouty-related, EVH1 domain-containing protein 3 isoform X4
XM_006539451.2 XP_006539514.1 sprouty-related, EVH1 domain-containing protein 3 isoform X1
XM_017321916.1 XP_017177405.1 sprouty-related, EVH1 domain-containing protein 3 isoform X7
XM_006539453.2 XP_006539516.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_006539455.4 XP_006539518.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3
XM_006539456.3 XP_006539519.1 sprouty-related, EVH1 domain-containing protein 3 isoform X3

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) SPRED3 XP_005616679.1
Pan troglodytes (chimpanzee) SPRED3 XP_524251.3
LOC715133 XP_001107519.1
Bos taurus (cattle) SPRED3 XP_003587312.2
Rattus norvegicus (Norway rat) Spred3 NP_001166815.1
Homo sapiens (human) SPRED3 NP_001035987.1
Mus musculus (house mouse) Spred3 NP_891557.3

Gene Ontology


Bibliography

Related articles in PubMed

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
Heng X, Guo Q, Leung AW, Li JY
eLife6(2017 May)

Fgf9 signalling stimulates Spred and Sprouty expression in embryonic mouse pancreas mesenchyme.
Sylvestersen KB, Herrera PL, Serup P, Rescan C
Gene expression patterns : GEP11(1-2)105-11(2011 Jan-Feb)

Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation.
Kato R, Nonami A, Taketomi T, Wakioka T, Kuroiwa A, Matsuda Y, Yoshimura A
Biochemical and biophysical research communications302(4)767-72(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spred3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spred3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu08251 NM_182927.3
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu08251 XM_006539454.4
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu08251 XM_006539456.3
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu08251 XM_006539455.4
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu08251 XM_006539453.3
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu37947 XM_006539452.2
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu72039 XM_030241962.1
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu81891 XM_017321915.2
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu37946 XM_006539451.2
Latest version!		 Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu81889 XM_011250410.2
Latest version!		 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OMu81890 XM_017321914.1
Latest version!		 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OMu81892 XM_017321916.1
Latest version!		 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
View Old Accession Versions >>
OMu37947 XM_006539452.1 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OMu08251 XM_006539454.3 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu81891 XM_017321915.1 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
OMu37946 XM_006539451.1 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OMu08251 XM_006539453.2 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu08251 XM_006539455.3 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu08251 XM_006539456.2 Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version NM_182927.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-23
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC062119.1, CF538218.1 and EL606697.1. On May 15, 2008 this sequence version replaced NM_182927.2. ##Evidence-Data-START## Transcript exon combination :: BC062119.1, CA324469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
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721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_891557.3
CDS133..1359
Translation

Target ORF information:

RefSeq Version NM_182927.3
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_182927.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539455.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539455.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539518.1
CDS196..1422
Translation

Target ORF information:

RefSeq Version XM_006539455.3
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539455.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539454.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539517.1
CDS241..1467
Translation

Target ORF information:

RefSeq Version XM_006539454.3
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539454.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539456.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006539456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539519.1
CDS97..1323
Translation

Target ORF information:

RefSeq Version XM_006539456.2
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539456.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539453.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539516.1
CDS87..1313
Translation

Target ORF information:

RefSeq Version XM_006539453.2
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539453.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539453.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539516.1
CDS643..1869
Translation

Target ORF information:

RefSeq Version XM_006539453.3
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539453.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539454.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539454.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539517.1
CDS240..1466
Translation

Target ORF information:

RefSeq Version XM_006539454.4
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539454.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539455.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539455.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539518.1
CDS582..1808
Translation

Target ORF information:

RefSeq Version XM_006539455.4
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539455.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08251
Clone ID Related Accession (Same CDS sequence) XM_006539455.3 , XM_006539453.3 , XM_006539454.3 , NM_182927.3 , XM_006539454.4 , XM_006539456.2 , XM_006539453.2 , XM_006539455.4 , XM_006539456.3
Accession Version XM_006539456.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539456.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539519.1
CDS113..1339
Translation

Target ORF information:

RefSeq Version XM_006539456.3
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539456.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCCGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTATGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAGC GGCTTCGGGA CCAGAAAACC 
ACCTTGGAGT GTACCCTGAG GCCAGGTTTG GTTTACAACA AAGTGAATCC CATCTTCCAC 
CATTGGAGCT TGGGGGACTG CAAGTTTGGG CTGACGTTTC AGAGTCCTGC AGAAGCGGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCTGCCCTGA GTCGAGGTTC GCTGACTCCT 
TCCTCTTCCT CTTCCTCCTC CTCCCCTTCC CAGGACACTG CAGAGACTCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCACA 
GCTCCCATTG CCACAGTGGA GTCAGCAGCT GCCTTCCCAC TGGCCACGCC CCCACAAAGG 
CGCCGCTCCT CTGCTCAGAG CTACCCTCCG CTTCTACCGT TCACTGGGAT TCCTGAACCT 
TCAGAGTCTT TAGCTGGGGC AGGGAGCCAG GGCTGGGGCA GCCGCGGCTA TGAGGACTAC 
CGGCGATCTG GACCACCGCC TCCACCTCTT GCCCTGTCTA CCTGCGTTGT GCGCTTTGCC 
AAGACTGGTG CATTGCGGGG TGCAGCCCTG GGTCCTCCAG TGTCACTCCC TGCCCCTCTC 
ACAGAGGCTG CACCCCCAGC ACCCCCTGCT CGTCCACCCC CAGGTCCAGG CCCCACCCCT 
GCTCCAGCCA AAGCCTCCCC AGAGGCTGAG GAGGCTGCAC GCTGTGTGCA CTGCCGAGCA 
CTCTTCCGCC GCCGGGCAGA TGGGCGTGGT GGTCGCTGCG CAGAGGCCCC TGACCCAGGT 
CGCCTGCTAG TGCGCCGGCT CAGCTGCTTG TGGTGTGCCG AGAGCTTGCT GTACCACTGC 
CTGTCAGACG CCGAGGGTGA CTTCTCGGAC CCGTGTGCTT GTGAGCCAGG CCACCCACGC 
CCTGCTGCGC GCTGGGCTGC TCTGGCTGCT CTGTCTCTGG CAGTGCCCTG TCTCTGTTGT 
TATGCGCCCC TGCGCGCCTG TCACTGGGTT GCAGCACGTT GTGGCTGTGC GGGCTGTGGG 
GGTCGCCACG AGGAGGCTGC ACGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu37947
Clone ID Related Accession (Same CDS sequence) XM_006539452.1 , XM_006539452.2
Accession Version XM_006539452.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTACGCCT GCCTTGCTTC TACCCCTCCC CCAAGCCGCC CCCTCCCCCA CACACATCTT 
CCAGGTTCCC CCTCCCTCCC CCCTCGCCCC GGCTCCCGGT GCCCGTCTCC AGCGCCGCCG 
GAGCCAGTGA GGGAGCGGGA GAGAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGCCGC 
GTCGCCGCCG CTCGGAGCCC GGCCGGAGCC TCCCAGACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539515.1
CDS423..1688
Translation

Target ORF information:

RefSeq Version XM_006539452.2
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539452.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTACGCCT GCCTTGCTTC TACCCCTCCC CCAAGCCGCC CCCTCCCCCA CACACATCTT 
CCAGGTTCCC CCTCCCTCCC CCCTCGCCCC GGCTCCCGGT GCCCGTCTCC AGCGCCGCCG 
GAGCCAGTGA GGGAGCGGGA GAGAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGCCGC 
GTCGCCGCCG CTCGGAGCCC GGCCGGAGCC TCCCAGACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu72039
Clone ID Related Accession (Same CDS sequence) XM_030241962.1
Accession Version XM_030241962.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 969bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGGTA 
TGTGTATGCA CCACCTGGCT CCTTGGTATC GGGCTCCCTA GAACTGAGCC ATGTGGTTGC 
TGGGAACCGA AACCCAGATG CTCCGTAAGT ACTCTCTTGC TTAGCTCTCT CTCCAACCAG 
AGCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030097822.1
CDS368..1336
Translation

Target ORF information:

RefSeq Version XM_030241962.1
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030241962.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGGTA 
TGTGTATGCA CCACCTGGCT CCTTGGTATC GGGCTCCCTA GAACTGAGCC ATGTGGTTGC 
TGGGAACCGA AACCCAGATG CTCCGTAAGT ACTCTCTTGC TTAGCTCTCT CTCCAACCAG 
AGCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu81891
Clone ID Related Accession (Same CDS sequence) XM_017321915.1 , XM_017321915.2
Accession Version XM_017321915.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGCATGTCAG CTCCCAGAAG 
CCACGGATCT TTTGCCAGTA CCTTGCTTCA CCTCCAGTCC CTAAAACAGT GCCCAGTACA 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017177404.1
CDS368..1153
Translation

Target ORF information:

RefSeq Version XM_017321915.2
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017321915.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGCATGTCAG CTCCCAGAAG 
CCACGGATCT TTTGCCAGTA CCTTGCTTCA CCTCCAGTCC CTAAAACAGT GCCCAGTACA 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu37946
Clone ID Related Accession (Same CDS sequence) XM_006539451.1 , XM_006539451.2
Accession Version XM_006539451.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539514.1
CDS368..1873
Translation

Target ORF information:

RefSeq Version XM_006539451.2
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related EVH1 domain containing 3 (Spred3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539451.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu37946
Clone ID Related Accession (Same CDS sequence) XM_006539451.1 , XM_006539451.2
Accession Version XM_006539451.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539514.1
CDS152..1657
Translation

Target ORF information:

RefSeq Version XM_006539451.1
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539451.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu37947
Clone ID Related Accession (Same CDS sequence) XM_006539452.1 , XM_006539452.2
Accession Version XM_006539452.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTACGCCT GCCTTGCTTC TACCCCTCCC CCAAGCCGCC CCCTCCCCCA CACACATCTT 
CCAGGTTCCC CCTCCCTCCC CCCTCGCCCC GGCTCCCGGT GCCCGTCTCC AGCGCCGCCG 
GAGCCAGTGA GGGAGCGGGA GAGAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGCCGC 
GTCGCCGCCG CTCGGAGCCC GGCCGGAGCC TCCCAGACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539515.1
CDS207..1472
Translation

Target ORF information:

RefSeq Version XM_006539452.1
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539452.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTACGCCT GCCTTGCTTC TACCCCTCCC CCAAGCCGCC CCCTCCCCCA CACACATCTT 
CCAGGTTCCC CCTCCCTCCC CCCTCGCCCC GGCTCCCGGT GCCCGTCTCC AGCGCCGCCG 
GAGCCAGTGA GGGAGCGGGA GAGAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGCCGC 
GTCGCCGCCG CTCGGAGCCC GGCCGGAGCC TCCCAGACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGAGC 
TACCCTCCGC TTCTACCGTT CACTGGGATT CCTGAACCTT CAGAGTCTTT AGCTGGGGCA 
GGGAGCCAGG GCTGGGGCAG CCGCGGCTAT GAGGACTACC GGCGATCTGG ACCACCGCCT 
CCACCTCTTG CCCTGTCTAC CTGCGTTGTG CGCTTTGCCA AGACTGGTGC ATTGCGGGGT 
GCAGCCCTGG GTCCTCCAGT GTCACTCCCT GCCCCTCTCA CAGAGGCTGC ACCCCCAGCA 
CCCCCTGCTC GTCCACCCCC AGGTCCAGGC CCCACCCCTG CTCCAGCCAA AGCCTCCCCA 
GAGGCTGAGG AGGCTGCACG CTGTGTGCAC TGCCGAGCAC TCTTCCGCCG CCGGGCAGAT 
GGGCGTGGTG GTCGCTGCGC AGAGGCCCCT GACCCAGGTC GCCTGCTAGT GCGCCGGCTC 
AGCTGCTTGT GGTGTGCCGA GAGCTTGCTG TACCACTGCC TGTCAGACGC CGAGGGTGAC 
TTCTCGGACC CGTGTGCTTG TGAGCCAGGC CACCCACGCC CTGCTGCGCG CTGGGCTGCT 
CTGGCTGCTC TGTCTCTGGC AGTGCCCTGT CTCTGTTGTT ATGCGCCCCT GCGCGCCTGT 
CACTGGGTTG CAGCACGTTG TGGCTGTGCG GGCTGTGGGG GTCGCCACGA GGAGGCTGCA 
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu81889
Clone ID Related Accession (Same CDS sequence) XM_011250410.2
Accession Version XM_011250410.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGGTA 
TGTGTATGCA CCACCTGGCT CCTTGGTATC GGGCTCCCTA GAACTGAGCC ATGTGGTTGC 
TGGGAACCGA AACCCAGATG CTCCGGATGC CCAAAGAGGC CAAAGAGCAT GGTGGATCCT 
CGGGTGCTTA CGAACCCCCA GAGGTCAGTG CTGGGAGCCA GCTTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248712.2
CDS152..1159
Translation

Target ORF information:

RefSeq Version XM_011250410.2
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250410.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGTCCCACGT GGACAGCGAC 
TCCTCCTCCA GTCACAGCCG CCAGGAGACT CCTCCCACAG CTCCCATTGC CACAGTGGAG 
TCAGCAGCTG CCTTCCCACT GGCCACGCCC CCACAAAGGC GCCGCTCCTC TGCTCAGGTA 
TGTGTATGCA CCACCTGGCT CCTTGGTATC GGGCTCCCTA GAACTGAGCC ATGTGGTTGC 
TGGGAACCGA AACCCAGATG CTCCGGATGC CCAAAGAGGC CAAAGAGCAT GGTGGATCCT 
CGGGTGCTTA CGAACCCCCA GAGGTCAGTG CTGGGAGCCA GCTTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu81890
Clone ID Related Accession (Same CDS sequence) XM_017321914.1
Accession Version XM_017321914.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGGTATGTGT ATGCACCACC 
TGGCTCCTTG GTATCGGGCT CCCTAGAACT GAGCCATGTG GTTGCTGGGA ACCGAAACCC 
AGATGCTCCG GATGCCCAAA GAGGCCAAAG AGCATGGTGG ATCCTCGGGT GCTTACGAAC 
CCCCAGAGGT CAGTGCTGGG AGCCAGCTTT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017177403.1
CDS152..1024
Translation

Target ORF information:

RefSeq Version XM_017321914.1
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017321914.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGGTATGTGT ATGCACCACC 
TGGCTCCTTG GTATCGGGCT CCCTAGAACT GAGCCATGTG GTTGCTGGGA ACCGAAACCC 
AGATGCTCCG GATGCCCAAA GAGGCCAAAG AGCATGGTGG ATCCTCGGGT GCTTACGAAC 
CCCCAGAGGT CAGTGCTGGG AGCCAGCTTT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu81891
Clone ID Related Accession (Same CDS sequence) XM_017321915.1 , XM_017321915.2
Accession Version XM_017321915.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGCATGTCAG CTCCCAGAAG 
CCACGGATCT TTTGCCAGTA CCTTGCTTCA CCTCCAGTCC CTAAAACAGT GCCCAGTACA 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017177404.1
CDS152..937
Translation

Target ORF information:

RefSeq Version XM_017321915.1
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017321915.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGCATGTCAG CTCCCAGAAG 
CCACGGATCT TTTGCCAGTA CCTTGCTTCA CCTCCAGTCC CTAAAACAGT GCCCAGTACA 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu81892
Clone ID Related Accession (Same CDS sequence) XM_017321916.1
Accession Version XM_017321916.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X7
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGGGATGCCC AAAGAGGCCA 
AAGAGCATGG TGGATCCTCG GGTGCTTACG AACCCCCAGA GGTCAGTGCT GGGAGCCAGC 
TTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017177405.1
CDS152..937
Translation

Target ORF information:

RefSeq Version XM_017321916.1
Organism Mus musculus(house mouse)
Definition Mus musculus sprouty-related, EVH1 domain containing 3 (Spred3), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017321916.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCTA TAGATGTTCG TTGTAGCCCT AGCCGGGCTC TAGGACCCAG CGGGCATGTA 
CGCCTGCCTT GCTTCTACCC CTCCCCCAAG CCGCCCCCTC CCCCACACAC ATCTTCCAGG 
TTCCCCCTCC CTCCCCCCTC GCCCCGGCTC CCGGTGCCCG TCTCCAGCGC CGCCGGAGCC 
AGTGAGGGAG CGGGAGAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GCCGCGTCGC 
CGCCGCTCGG AGCCCGGCCG GAGCCTCCCA GGCAGGTACA TGGTGCGGGT CCGAGCCGTG 
GTGATGGCCC GAGATGACTC CAGTGGGGGC TGGCTGCCTG TGGGGGGCGG GGGCCTCAGC 
CAGGTGAGCG TATGTCGGGT CCGAGGGGCC AGGCCCGAGG GGGGGGCCCG CCAGGGGCAC 
TACGTCATCC ACGGGGAGCG GCTTCGGGAC CAGAAAACCA CCTTGGAGTG TACCCTGAGG 
CCAGGTTTGG TTTACAACAA AGTGAATCCC ATCTTCCACC ATTGGAGCTT GGGGGACTGC 
AAGTTTGGGC TGACGTTTCA GAGTCCTGCA GAAGCGGATG AGTTCCAGAA GAGCCTGCTG 
GCTGCGCTGG CTGCCCTGAG TCGAGGTTCG CTGACTCCTT CCTCTTCCTC TTCCTCCTCC 
TCCCCTTCCC AGGACACTGC AGAGACTCCC TGCCCTCTGA CGGGATGCCC AAAGAGGCCA 
AAGAGCATGG TGGATCCTCG GGTGCTTACG AACCCCCAGA GGTCAGTGCT GGGAGCCAGC 
TTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
eLife6(2017 May)
Heng X,Guo Q,Leung AW,Li JY


book

Fgf9 signalling stimulates Spred and Sprouty expression in embryonic mouse pancreas mesenchyme.
Gene expression patterns : GEP11(1-2)105-11(2011 Jan-Feb)
Sylvestersen KB,Herrera PL,Serup P,Rescan C


book

Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation.
Biochemical and biophysical research communications302(4)767-72(2003 Mar)
Kato R,Nonami A,Taketomi T,Wakioka T,Kuroiwa A,Matsuda Y,Yoshimura A