Sf3b3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Sf3b3
Entrez Gene ID	101943
Full Name	splicing factor 3b, subunit 3
Synonyms	1810061H24Rik,5730409A01Rik,AA409318,D8Ertd633e,RSE1,SAP130,mKIAA0017
General protein information	Preferred Names splicing factor 3b, subunit 3 Names splicing factor 3B subunit 3 SAP 130 SF3b130 pre-mRNA-splicing factor SF3b 130 kDa subunit spliceosome-associated protein 130
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 E1\|8 57.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530518.2	XP_006530581.1	splicing factor 3B subunit 3 isoform X1
XM_017312494.1	XP_017167983.1	splicing factor 3B subunit 3 isoform X2
NM_133953.2	NP_598714.1	splicing factor 3B subunit 3
XM_006530518.4	XP_006530581.1	splicing factor 3B subunit 3 isoform X1
XM_006530518.3	XP_006530581.1	splicing factor 3B subunit 3 isoform X1
XM_006530520.3	XP_006530583.1	splicing factor 3B subunit 3 isoform X3
XM_030243208.1	XP_030099068.1	splicing factor 3B subunit 3 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SF3B3	XP_536791.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005549	XP_315551.1
Homo sapiens (human)	SF3B3	NP_036558.3
Bos taurus (cattle)	SF3B3	NP_001071319.1
Rattus norvegicus (Norway rat)	Sf3b3	NP_001099657.2
Gallus gallus (chicken)	SF3B3	XP_004944176.1
Danio rerio (zebrafish)	LOC100334114	XP_002667683.2
Magnaporthe oryzae (rice blast fungus)	MGG_06457	XP_003717109.1
Neurospora crassa	NCU00396	XP_957282.2
Xenopus tropicalis (tropical clawed frog)	sf3b3	XP_002931705.1
	SF3B3	XP_002802591.1
Mus musculus (house mouse)	Sf3b3	NP_598714.1
Caenorhabditis elegans (roundworm)	teg-4	NP_491953.1
Arabidopsis thaliana (thale cress)	AT3G55220	NP_567016.1
Pan troglodytes (chimpanzee)	SF3B3	XP_511081.3
Danio rerio (zebrafish)	sf3b3	NP_998668.1
Drosophila melanogaster (fruit fly)	CG13900	NP_728546.1
Schizosaccharomyces pombe (fission yeast)	prp12	NP_594414.1
Arabidopsis thaliana (thale cress)	AT3G55200	NP_567015.1
Oryza sativa (rice)	Os02g0137400	NP_001045829.2

Gene Ontology
Bibliography

Related articles in PubMed

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA
BMC developmental biology514(2005 Jul)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sf3b3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu41119	XM_006530520.3 Latest version!	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu41119	XM_030243208.1 Latest version!	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu82317	XM_017312494.1 Latest version!	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu10398	NM_133953.2 Latest version!	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OMu10398	XM_006530518.4 Latest version!	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu10398	XM_006530518.2	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OMu10398	XM_006530518.3	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3b3 ( XM_006530520.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530583.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu41119
Clone ID Related Accession (Same CDS sequence)	XM_006530520.3 , XM_030243208.1
Accession Version	XM_006530520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530520.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CAGGACAGTT GAATGAGTAC ACGGAGCGGA AAGAGATGTC AGCAGATGTG 
GTGTGCATGA GTCTGGCCAA CGTGCCCCCT GGAGAACAGC GATCTCGGTT CCTGGCTGTG 
GGGCTGGTGG ACAACACTGT CAGAATCATC TCCCTCGACC CATCAGACTG TTTACAGCCT 
CTGAGTATGC AGGCTCTCCC AGCCCAGCCT GAGTCTCTAT GTATTGTGGA AATGGGTGGG 
ACTGAGAAAC AAGATGAGCT GGGTGAGAGA GGCTCTATTG GCTTCCTGTA CCTGAACATT 
GGACTACAGA ACGGTGTACT GCTGAGGACT GTCCTAGACC CTGTTACTGG GGACCTGTCT 
GACACCCGCA CTAGGTATCT GGGGTCCCGT CCTGTGAAAC TCTTCCGGGT CCGGATGCAA 
GGCCAGGAGG CTGTTCTGGC TATGTCCAGT CGCTCATGGC TGAGCTACTC TTACCAGTCT 
CGCTTCCATC TCACCCCTCT GTCTTATGAG ACCCTGGAAT TTGCATCGGG CTTTGCCTCT 
GAACAGTGTC CCGAGGGCAT TGTTGCCATC TCCACCAACA CTTTGAGGAT TTTGGCTTTA 
GAGAAGCTTG GTGCTGTCTT CAATCAAGTA GCCTTCCCAT TGCAGTACAC ACCCAGGAAG 
TTTGTCATCC ACCCTGAGAG TAACAACCTT ATCATCATTG AGACGGATCA CAATGCCTAC 
ACTGAGGCCA CAAAAGCTCA GAGAAAGCAG CAGATGGCTG AGGAAATGGT AGAAGCAGCA 
GGGGAGGATG AGCGGGAGTT GGCTGCAGAA ATGGCAGCAG CATTCCTCAA TGAAAACCTC 
CCAGAATCCA TTTTTGGAGC TCCCAAGGCT GGCAATGGGC AGTGGGCCTC TGTGATTCGG 
GTGATGAACC CTATCCAGGG GAATACACTA GACCTTGTCC AGTTGGAGCA AAACGAGGCA 
GCCTTTAGTG TGGCAGTGTG TAGGTTCTCC AACACTGGTG AAGATTGGTA TGTGCTGGTG 
GGTGTTGCCA AGGACTTGAT ACTGAGCCCT CGATCTGTGG CAGGGGGCTT TGTGTATACG 
TACAAGCTTG TGAACAACGG GGAGAAGTTG GAGTTTCTGC ACAAGACTCC AGTGGAAGAG 
GTCCCTGCTG CCATTGCCCC ATTCCAGGGG AGGGTACTGA TTGGTGTCGG GAAGCTTTTA 
CGAGTTTATG ACCTGGGGAA GAAGAAGTTA CTTCGAAAGT GTGAGAATAA GCATATTGCC 
AATTACATAT CTGGGATCCA GACAATTGGT CACAGGGTAA TTGTGTCTGA TGTCCAAGAA 
AGTTTCATCT GGGTTCGATA CAAGCGTAAC GAGAACCAGC TCATTATCTT TGCTGATGAC 
ACCTATCCCA GATGGGTCAC CACAGCCAGC CTTCTCGACT ATGACACTGT GGCTGGGGCA 
GACAAGTTTG GCAACATTTG TGTGGTGAGG CTCCCACCTA ACACCAACGA TGAGGTGGAT 
GAAGATCCCA CTGGAAACAA AGCCCTGTGG GACCGGGGCT TGCTCAATGG GGCTTCACAG 
AAGGCAGAGG TCATCATGAA CTACCACGTT GGAGAGACCG TGCTGTCGCT ACAGAAGACC 
ACACTGATCC CTGGAGGCTC AGAGTCCCTT GTCTATACCA CTTTATCTGG AGGAATTGGC 
ATCCTTGTCC CTTTCACGTC TCACGAGGAC CATGACTTCT TCCAGCATGT TGAAATGCAC 
CTGCGGTCTG AGCATCCCCC TCTTTGTGGA CGGGACCACC TGAGCTTTCG TTCTTACTAT 
TTCCCTGTTA AGAATGTGAT TGATGGAGAC CTTTGTGAGC AGTTCAACTC CATGGAACCC 
AACAAGCAGA AGAACGTCTC TGAGGAGCTG GACCGAACCC CACCTGAAGT GTCCAAGAAG 
CTTGAGGACA TTAGGACCCG CTATGCCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530583.1
CDS	1798..3750
Translation	MDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	XM_006530520.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530520.3

ORF Insert Sequence:

ATGGATCCTT CAGGACAGTT GAATGAGTAC ACGGAGCGGA AAGAGATGTC AGCAGATGTG 
GTGTGCATGA GTCTGGCCAA CGTGCCCCCT GGAGAACAGC GATCTCGGTT CCTGGCTGTG 
GGGCTGGTGG ACAACACTGT CAGAATCATC TCCCTCGACC CATCAGACTG TTTACAGCCT 
CTGAGTATGC AGGCTCTCCC AGCCCAGCCT GAGTCTCTAT GTATTGTGGA AATGGGTGGG 
ACTGAGAAAC AAGATGAGCT GGGTGAGAGA GGCTCTATTG GCTTCCTGTA CCTGAACATT 
GGACTACAGA ACGGTGTACT GCTGAGGACT GTCCTAGACC CTGTTACTGG GGACCTGTCT 
GACACCCGCA CTAGGTATCT GGGGTCCCGT CCTGTGAAAC TCTTCCGGGT CCGGATGCAA 
GGCCAGGAGG CTGTTCTGGC TATGTCCAGT CGCTCATGGC TGAGCTACTC TTACCAGTCT 
CGCTTCCATC TCACCCCTCT GTCTTATGAG ACCCTGGAAT TTGCATCGGG CTTTGCCTCT 
GAACAGTGTC CCGAGGGCAT TGTTGCCATC TCCACCAACA CTTTGAGGAT TTTGGCTTTA 
GAGAAGCTTG GTGCTGTCTT CAATCAAGTA GCCTTCCCAT TGCAGTACAC ACCCAGGAAG 
TTTGTCATCC ACCCTGAGAG TAACAACCTT ATCATCATTG AGACGGATCA CAATGCCTAC 
ACTGAGGCCA CAAAAGCTCA GAGAAAGCAG CAGATGGCTG AGGAAATGGT AGAAGCAGCA 
GGGGAGGATG AGCGGGAGTT GGCTGCAGAA ATGGCAGCAG CATTCCTCAA TGAAAACCTC 
CCAGAATCCA TTTTTGGAGC TCCCAAGGCT GGCAATGGGC AGTGGGCCTC TGTGATTCGG 
GTGATGAACC CTATCCAGGG GAATACACTA GACCTTGTCC AGTTGGAGCA AAACGAGGCA 
GCCTTTAGTG TGGCAGTGTG TAGGTTCTCC AACACTGGTG AAGATTGGTA TGTGCTGGTG 
GGTGTTGCCA AGGACTTGAT ACTGAGCCCT CGATCTGTGG CAGGGGGCTT TGTGTATACG 
TACAAGCTTG TGAACAACGG GGAGAAGTTG GAGTTTCTGC ACAAGACTCC AGTGGAAGAG 
GTCCCTGCTG CCATTGCCCC ATTCCAGGGG AGGGTACTGA TTGGTGTCGG GAAGCTTTTA 
CGAGTTTATG ACCTGGGGAA GAAGAAGTTA CTTCGAAAGT GTGAGAATAA GCATATTGCC 
AATTACATAT CTGGGATCCA GACAATTGGT CACAGGGTAA TTGTGTCTGA TGTCCAAGAA 
AGTTTCATCT GGGTTCGATA CAAGCGTAAC GAGAACCAGC TCATTATCTT TGCTGATGAC 
ACCTATCCCA GATGGGTCAC CACAGCCAGC CTTCTCGACT ATGACACTGT GGCTGGGGCA 
GACAAGTTTG GCAACATTTG TGTGGTGAGG CTCCCACCTA ACACCAACGA TGAGGTGGAT 
GAAGATCCCA CTGGAAACAA AGCCCTGTGG GACCGGGGCT TGCTCAATGG GGCTTCACAG 
AAGGCAGAGG TCATCATGAA CTACCACGTT GGAGAGACCG TGCTGTCGCT ACAGAAGACC 
ACACTGATCC CTGGAGGCTC AGAGTCCCTT GTCTATACCA CTTTATCTGG AGGAATTGGC 
ATCCTTGTCC CTTTCACGTC TCACGAGGAC CATGACTTCT TCCAGCATGT TGAAATGCAC 
CTGCGGTCTG AGCATCCCCC TCTTTGTGGA CGGGACCACC TGAGCTTTCG TTCTTACTAT 
TTCCCTGTTA AGAATGTGAT TGATGGAGAC CTTTGTGAGC AGTTCAACTC CATGGAACCC 
AACAAGCAGA AGAACGTCTC TGAGGAGCTG GACCGAACCC CACCTGAAGT GTCCAAGAAG 
CTTGAGGACA TTAGGACCCG CTATGCCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( XM_030243208.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099068.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu41119
Clone ID Related Accession (Same CDS sequence)	XM_006530520.3 , XM_030243208.1
Accession Version	XM_030243208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CAGGACAGTT GAATGAGTAC ACGGAGCGGA AAGAGATGTC AGCAGATGTG 
GTGTGCATGA GTCTGGCCAA CGTGCCCCCT GGAGAACAGC GATCTCGGTT CCTGGCTGTG 
GGGCTGGTGG ACAACACTGT CAGAATCATC TCCCTCGACC CATCAGACTG TTTACAGCCT 
CTGAGTATGC AGGCTCTCCC AGCCCAGCCT GAGTCTCTAT GTATTGTGGA AATGGGTGGG 
ACTGAGAAAC AAGATGAGCT GGGTGAGAGA GGCTCTATTG GCTTCCTGTA CCTGAACATT 
GGACTACAGA ACGGTGTACT GCTGAGGACT GTCCTAGACC CTGTTACTGG GGACCTGTCT 
GACACCCGCA CTAGGTATCT GGGGTCCCGT CCTGTGAAAC TCTTCCGGGT CCGGATGCAA 
GGCCAGGAGG CTGTTCTGGC TATGTCCAGT CGCTCATGGC TGAGCTACTC TTACCAGTCT 
CGCTTCCATC TCACCCCTCT GTCTTATGAG ACCCTGGAAT TTGCATCGGG CTTTGCCTCT 
GAACAGTGTC CCGAGGGCAT TGTTGCCATC TCCACCAACA CTTTGAGGAT TTTGGCTTTA 
GAGAAGCTTG GTGCTGTCTT CAATCAAGTA GCCTTCCCAT TGCAGTACAC ACCCAGGAAG 
TTTGTCATCC ACCCTGAGAG TAACAACCTT ATCATCATTG AGACGGATCA CAATGCCTAC 
ACTGAGGCCA CAAAAGCTCA GAGAAAGCAG CAGATGGCTG AGGAAATGGT AGAAGCAGCA 
GGGGAGGATG AGCGGGAGTT GGCTGCAGAA ATGGCAGCAG CATTCCTCAA TGAAAACCTC 
CCAGAATCCA TTTTTGGAGC TCCCAAGGCT GGCAATGGGC AGTGGGCCTC TGTGATTCGG 
GTGATGAACC CTATCCAGGG GAATACACTA GACCTTGTCC AGTTGGAGCA AAACGAGGCA 
GCCTTTAGTG TGGCAGTGTG TAGGTTCTCC AACACTGGTG AAGATTGGTA TGTGCTGGTG 
GGTGTTGCCA AGGACTTGAT ACTGAGCCCT CGATCTGTGG CAGGGGGCTT TGTGTATACG 
TACAAGCTTG TGAACAACGG GGAGAAGTTG GAGTTTCTGC ACAAGACTCC AGTGGAAGAG 
GTCCCTGCTG CCATTGCCCC ATTCCAGGGG AGGGTACTGA TTGGTGTCGG GAAGCTTTTA 
CGAGTTTATG ACCTGGGGAA GAAGAAGTTA CTTCGAAAGT GTGAGAATAA GCATATTGCC 
AATTACATAT CTGGGATCCA GACAATTGGT CACAGGGTAA TTGTGTCTGA TGTCCAAGAA 
AGTTTCATCT GGGTTCGATA CAAGCGTAAC GAGAACCAGC TCATTATCTT TGCTGATGAC 
ACCTATCCCA GATGGGTCAC CACAGCCAGC CTTCTCGACT ATGACACTGT GGCTGGGGCA 
GACAAGTTTG GCAACATTTG TGTGGTGAGG CTCCCACCTA ACACCAACGA TGAGGTGGAT 
GAAGATCCCA CTGGAAACAA AGCCCTGTGG GACCGGGGCT TGCTCAATGG GGCTTCACAG 
AAGGCAGAGG TCATCATGAA CTACCACGTT GGAGAGACCG TGCTGTCGCT ACAGAAGACC 
ACACTGATCC CTGGAGGCTC AGAGTCCCTT GTCTATACCA CTTTATCTGG AGGAATTGGC 
ATCCTTGTCC CTTTCACGTC TCACGAGGAC CATGACTTCT TCCAGCATGT TGAAATGCAC 
CTGCGGTCTG AGCATCCCCC TCTTTGTGGA CGGGACCACC TGAGCTTTCG TTCTTACTAT 
TTCCCTGTTA AGAATGTGAT TGATGGAGAC CTTTGTGAGC AGTTCAACTC CATGGAACCC 
AACAAGCAGA AGAACGTCTC TGAGGAGCTG GACCGAACCC CACCTGAAGT GTCCAAGAAG 
CTTGAGGACA TTAGGACCCG CTATGCCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099068.1
CDS	1930..3882
Translation	MDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	XM_030243208.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243208.1

ORF Insert Sequence:

ATGGATCCTT CAGGACAGTT GAATGAGTAC ACGGAGCGGA AAGAGATGTC AGCAGATGTG 
GTGTGCATGA GTCTGGCCAA CGTGCCCCCT GGAGAACAGC GATCTCGGTT CCTGGCTGTG 
GGGCTGGTGG ACAACACTGT CAGAATCATC TCCCTCGACC CATCAGACTG TTTACAGCCT 
CTGAGTATGC AGGCTCTCCC AGCCCAGCCT GAGTCTCTAT GTATTGTGGA AATGGGTGGG 
ACTGAGAAAC AAGATGAGCT GGGTGAGAGA GGCTCTATTG GCTTCCTGTA CCTGAACATT 
GGACTACAGA ACGGTGTACT GCTGAGGACT GTCCTAGACC CTGTTACTGG GGACCTGTCT 
GACACCCGCA CTAGGTATCT GGGGTCCCGT CCTGTGAAAC TCTTCCGGGT CCGGATGCAA 
GGCCAGGAGG CTGTTCTGGC TATGTCCAGT CGCTCATGGC TGAGCTACTC TTACCAGTCT 
CGCTTCCATC TCACCCCTCT GTCTTATGAG ACCCTGGAAT TTGCATCGGG CTTTGCCTCT 
GAACAGTGTC CCGAGGGCAT TGTTGCCATC TCCACCAACA CTTTGAGGAT TTTGGCTTTA 
GAGAAGCTTG GTGCTGTCTT CAATCAAGTA GCCTTCCCAT TGCAGTACAC ACCCAGGAAG 
TTTGTCATCC ACCCTGAGAG TAACAACCTT ATCATCATTG AGACGGATCA CAATGCCTAC 
ACTGAGGCCA CAAAAGCTCA GAGAAAGCAG CAGATGGCTG AGGAAATGGT AGAAGCAGCA 
GGGGAGGATG AGCGGGAGTT GGCTGCAGAA ATGGCAGCAG CATTCCTCAA TGAAAACCTC 
CCAGAATCCA TTTTTGGAGC TCCCAAGGCT GGCAATGGGC AGTGGGCCTC TGTGATTCGG 
GTGATGAACC CTATCCAGGG GAATACACTA GACCTTGTCC AGTTGGAGCA AAACGAGGCA 
GCCTTTAGTG TGGCAGTGTG TAGGTTCTCC AACACTGGTG AAGATTGGTA TGTGCTGGTG 
GGTGTTGCCA AGGACTTGAT ACTGAGCCCT CGATCTGTGG CAGGGGGCTT TGTGTATACG 
TACAAGCTTG TGAACAACGG GGAGAAGTTG GAGTTTCTGC ACAAGACTCC AGTGGAAGAG 
GTCCCTGCTG CCATTGCCCC ATTCCAGGGG AGGGTACTGA TTGGTGTCGG GAAGCTTTTA 
CGAGTTTATG ACCTGGGGAA GAAGAAGTTA CTTCGAAAGT GTGAGAATAA GCATATTGCC 
AATTACATAT CTGGGATCCA GACAATTGGT CACAGGGTAA TTGTGTCTGA TGTCCAAGAA 
AGTTTCATCT GGGTTCGATA CAAGCGTAAC GAGAACCAGC TCATTATCTT TGCTGATGAC 
ACCTATCCCA GATGGGTCAC CACAGCCAGC CTTCTCGACT ATGACACTGT GGCTGGGGCA 
GACAAGTTTG GCAACATTTG TGTGGTGAGG CTCCCACCTA ACACCAACGA TGAGGTGGAT 
GAAGATCCCA CTGGAAACAA AGCCCTGTGG GACCGGGGCT TGCTCAATGG GGCTTCACAG 
AAGGCAGAGG TCATCATGAA CTACCACGTT GGAGAGACCG TGCTGTCGCT ACAGAAGACC 
ACACTGATCC CTGGAGGCTC AGAGTCCCTT GTCTATACCA CTTTATCTGG AGGAATTGGC 
ATCCTTGTCC CTTTCACGTC TCACGAGGAC CATGACTTCT TCCAGCATGT TGAAATGCAC 
CTGCGGTCTG AGCATCCCCC TCTTTGTGGA CGGGACCACC TGAGCTTTCG TTCTTACTAT 
TTCCCTGTTA AGAATGTGAT TGATGGAGAC CTTTGTGAGC AGTTCAACTC CATGGAACCC 
AACAAGCAGA AGAACGTCTC TGAGGAGCTG GACCGAACCC CACCTGAAGT GTCCAAGAAG 
CTTGAGGACA TTAGGACCCG CTATGCCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( XM_017312494.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167983.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X2, mRNA.

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CloneID	OMu82317
Clone ID Related Accession (Same CDS sequence)	XM_017312494.1
Accession Version	XM_017312494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATACTA CCAATACCAA GCCTTTTTTT CTCTTCCACA GGTGGCTGGT GAAGGTGCTG 
ATTATCACAG ATGAGTTTGA TGCCTACATC ATTGTGTCTT TCGTGAATGC CACACTTGTG 
CTATCTATCG GAGAGACTGT GGAAGAAGTG ACTGACTCTG GCTTCTTGGG CACCACTCCA 
ACCTTGTCCT GCTCCTTGTT AGGAGATGAT GCACTGGTGC AGGTGTATCC AGATGGCATC 
CGACACATAC GAGCAGATAA AAGAGTTAAT GAGTGGAAGA CTCCCGGCAA GAAAACGATT 
GTGAAGTGTG CAGTGAACCA GCGACAAGTG GTGATCGCCC TGACTGGGGG AGAGCTGGTC 
TATTTCGAGA TGGATCCTTC AGGACAGTTG AATGAGTACA CGGAGCGGAA AGAGATGTCA 
GCAGATGTGG TGTGCATGAG TCTGGCCAAC GTGCCCCCTG GAGAACAGCG ATCTCGGTTC 
CTGGCTGTGG GGCTGGTGGA CAACACTGTC AGAATCATCT CCCTCGACCC ATCAGACTGT 
TTACAGCCTC TGAGTATGCA GGCTCTCCCA GCCCAGCCTG AGTCTCTATG TATTGTGGAA 
ATGGGTGGGA CTGAGAAACA AGATGAGCTG GGTGAGAGAG GCTCTATTGG CTTCCTGTAC 
CTGAACATTG GACTACAGAA CGGTGTACTG CTGAGGACTG TCCTAGACCC TGTTACTGGG 
GACCTGTCTG ACACCCGCAC TAGGTATCTG GGGTCCCGTC CTGTGAAACT CTTCCGGGTC 
CGGATGCAAG GCCAGGAGGC TGTTCTGGCT ATGTCCAGTC GCTCATGGCT GAGCTACTCT 
TACCAGTCTC GCTTCCATCT CACCCCTCTG TCTTATGAGA CCCTGGAATT TGCATCGGGC 
TTTGCCTCTG AACAGTGTCC CGAGGGCATT GTTGCCATCT CCACCAACAC TTTGAGGATT 
TTGGCTTTAG AGAAGCTTGG TGCTGTCTTC AATCAAGTAG CCTTCCCATT GCAGTACACA 
CCCAGGAAGT TTGTCATCCA CCCTGAGAGT AACAACCTTA TCATCATTGA GACGGATCAC 
AATGCCTACA CTGAGGCCAC AAAAGCTCAG AGAAAGCAGC AGATGGCTGA GGAAATGGTA 
GAAGCAGCAG GGGAGGATGA GCGGGAGTTG GCTGCAGAAA TGGCAGCAGC ATTCCTCAAT 
GAAAACCTCC CAGAATCCAT TTTTGGAGCT CCCAAGGCTG GCAATGGGCA GTGGGCCTCT 
GTGATTCGGG TGATGAACCC TATCCAGGGG AATACACTAG ACCTTGTCCA GTTGGAGCAA 
AACGAGGCAG CCTTTAGTGT GGCAGTGTGT AGGTTCTCCA ACACTGGTGA AGATTGGTAT 
GTGCTGGTGG GTGTTGCCAA GGACTTGATA CTGAGCCCTC GATCTGTGGC AGGGGGCTTT 
GTGTATACGT ACAAGCTTGT GAACAACGGG GAGAAGTTGG AGTTTCTGCA CAAGACTCCA 
GTGGAAGAGG TCCCTGCTGC CATTGCCCCA TTCCAGGGGA GGGTACTGAT TGGTGTCGGG 
AAGCTTTTAC GAGTTTATGA CCTGGGGAAG AAGAAGTTAC TTCGAAAGTG TGAGAATAAG 
CATATTGCCA ATTACATATC TGGGATCCAG ACAATTGGTC ACAGGGTAAT TGTGTCTGAT 
GTCCAAGAAA GTTTCATCTG GGTTCGATAC AAGCGTAACG AGAACCAGCT CATTATCTTT 
GCTGATGACA CCTATCCCAG ATGGGTCACC ACAGCCAGCC TTCTCGACTA TGACACTGTG 
GCTGGGGCAG ACAAGTTTGG CAACATTTGT GTGGTGAGGC TCCCACCTAA CACCAACGAT 
GAGGTGGATG AAGATCCCAC TGGAAACAAA GCCCTGTGGG ACCGGGGCTT GCTCAATGGG 
GCTTCACAGA AGGCAGAGGT CATCATGAAC TACCACGTTG GAGAGACCGT GCTGTCGCTA 
CAGAAGACCA CACTGATCCC TGGAGGCTCA GAGTCCCTTG TCTATACCAC TTTATCTGGA 
GGAATTGGCA TCCTTGTCCC TTTCACGTCT CACGAGGACC ATGACTTCTT CCAGCATGTT 
GAAATGCACC TGCGGTCTGA GCATCCCCCT CTTTGTGGAC GGGACCACCT GAGCTTTCGT 
TCTTACTATT TCCCTGTTAA GAATGTGATT GATGGAGACC TTTGTGAGCA GTTCAACTCC 
ATGGAACCCA ACAAGCAGAA GAACGTCTCT GAGGAGCTGG ACCGAACCCC ACCTGAAGTG 
TCCAAGAAGC TTGAGGACAT TAGGACCCGC TATGCCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167983.1
CDS	3365..5686
Translation	MHTTNTKPFFLFHRWLVKVLIITDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	XM_017312494.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312494.1

ORF Insert Sequence:

ATGCATACTA CCAATACCAA GCCTTTTTTT CTCTTCCACA GGTGGCTGGT GAAGGTGCTG 
ATTATCACAG ATGAGTTTGA TGCCTACATC ATTGTGTCTT TCGTGAATGC CACACTTGTG 
CTATCTATCG GAGAGACTGT GGAAGAAGTG ACTGACTCTG GCTTCTTGGG CACCACTCCA 
ACCTTGTCCT GCTCCTTGTT AGGAGATGAT GCACTGGTGC AGGTGTATCC AGATGGCATC 
CGACACATAC GAGCAGATAA AAGAGTTAAT GAGTGGAAGA CTCCCGGCAA GAAAACGATT 
GTGAAGTGTG CAGTGAACCA GCGACAAGTG GTGATCGCCC TGACTGGGGG AGAGCTGGTC 
TATTTCGAGA TGGATCCTTC AGGACAGTTG AATGAGTACA CGGAGCGGAA AGAGATGTCA 
GCAGATGTGG TGTGCATGAG TCTGGCCAAC GTGCCCCCTG GAGAACAGCG ATCTCGGTTC 
CTGGCTGTGG GGCTGGTGGA CAACACTGTC AGAATCATCT CCCTCGACCC ATCAGACTGT 
TTACAGCCTC TGAGTATGCA GGCTCTCCCA GCCCAGCCTG AGTCTCTATG TATTGTGGAA 
ATGGGTGGGA CTGAGAAACA AGATGAGCTG GGTGAGAGAG GCTCTATTGG CTTCCTGTAC 
CTGAACATTG GACTACAGAA CGGTGTACTG CTGAGGACTG TCCTAGACCC TGTTACTGGG 
GACCTGTCTG ACACCCGCAC TAGGTATCTG GGGTCCCGTC CTGTGAAACT CTTCCGGGTC 
CGGATGCAAG GCCAGGAGGC TGTTCTGGCT ATGTCCAGTC GCTCATGGCT GAGCTACTCT 
TACCAGTCTC GCTTCCATCT CACCCCTCTG TCTTATGAGA CCCTGGAATT TGCATCGGGC 
TTTGCCTCTG AACAGTGTCC CGAGGGCATT GTTGCCATCT CCACCAACAC TTTGAGGATT 
TTGGCTTTAG AGAAGCTTGG TGCTGTCTTC AATCAAGTAG CCTTCCCATT GCAGTACACA 
CCCAGGAAGT TTGTCATCCA CCCTGAGAGT AACAACCTTA TCATCATTGA GACGGATCAC 
AATGCCTACA CTGAGGCCAC AAAAGCTCAG AGAAAGCAGC AGATGGCTGA GGAAATGGTA 
GAAGCAGCAG GGGAGGATGA GCGGGAGTTG GCTGCAGAAA TGGCAGCAGC ATTCCTCAAT 
GAAAACCTCC CAGAATCCAT TTTTGGAGCT CCCAAGGCTG GCAATGGGCA GTGGGCCTCT 
GTGATTCGGG TGATGAACCC TATCCAGGGG AATACACTAG ACCTTGTCCA GTTGGAGCAA 
AACGAGGCAG CCTTTAGTGT GGCAGTGTGT AGGTTCTCCA ACACTGGTGA AGATTGGTAT 
GTGCTGGTGG GTGTTGCCAA GGACTTGATA CTGAGCCCTC GATCTGTGGC AGGGGGCTTT 
GTGTATACGT ACAAGCTTGT GAACAACGGG GAGAAGTTGG AGTTTCTGCA CAAGACTCCA 
GTGGAAGAGG TCCCTGCTGC CATTGCCCCA TTCCAGGGGA GGGTACTGAT TGGTGTCGGG 
AAGCTTTTAC GAGTTTATGA CCTGGGGAAG AAGAAGTTAC TTCGAAAGTG TGAGAATAAG 
CATATTGCCA ATTACATATC TGGGATCCAG ACAATTGGTC ACAGGGTAAT TGTGTCTGAT 
GTCCAAGAAA GTTTCATCTG GGTTCGATAC AAGCGTAACG AGAACCAGCT CATTATCTTT 
GCTGATGACA CCTATCCCAG ATGGGTCACC ACAGCCAGCC TTCTCGACTA TGACACTGTG 
GCTGGGGCAG ACAAGTTTGG CAACATTTGT GTGGTGAGGC TCCCACCTAA CACCAACGAT 
GAGGTGGATG AAGATCCCAC TGGAAACAAA GCCCTGTGGG ACCGGGGCTT GCTCAATGGG 
GCTTCACAGA AGGCAGAGGT CATCATGAAC TACCACGTTG GAGAGACCGT GCTGTCGCTA 
CAGAAGACCA CACTGATCCC TGGAGGCTCA GAGTCCCTTG TCTATACCAC TTTATCTGGA 
GGAATTGGCA TCCTTGTCCC TTTCACGTCT CACGAGGACC ATGACTTCTT CCAGCATGTT 
GAAATGCACC TGCGGTCTGA GCATCCCCCT CTTTGTGGAC GGGACCACCT GAGCTTTCGT 
TCTTACTATT TCCCTGTTAA GAATGTGATT GATGGAGACC TTTGTGAGCA GTTCAACTCC 
ATGGAACCCA ACAAGCAGAA GAACGTCTCT GAGGAGCTGG ACCGAACCCC ACCTGAAGTG 
TCCAAGAAGC TTGAGGACAT TAGGACCCGC TATGCCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( NM_133953.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598714.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), mRNA.

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CloneID	OMu10398
Clone ID Related Accession (Same CDS sequence)	XM_006530518.2 , NM_133953.2 , XM_006530518.4 , XM_006530518.3
Accession Version	NM_133953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY126146.1, AK128988.1 and BC042580.1. On Nov 30, 2006 this sequence version replaced NM_133953.1. ##Evidence-Data-START## Transcript exon combination :: AK164199.1, AK128988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598714.1
CDS	197..3850
Translation	MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	NM_133953.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133953.2

ORF Insert Sequence:

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( XM_006530518.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530581.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.

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CloneID	OMu10398
Clone ID Related Accession (Same CDS sequence)	XM_006530518.2 , NM_133953.2 , XM_006530518.4 , XM_006530518.3
Accession Version	XM_006530518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	splicing factor 3B subunit 3 isoform X1
Comment

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006530581.1

Translation

MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	XM_006530518.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530518.2

ORF Insert Sequence:

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( XM_006530518.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530581.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10398
Clone ID Related Accession (Same CDS sequence)	XM_006530518.2 , NM_133953.2 , XM_006530518.4 , XM_006530518.3
Accession Version	XM_006530518.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530581.1
CDS	172..3825
Translation	MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	XM_006530518.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530518.3

ORF Insert Sequence:

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( XM_006530518.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530581.1 Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.

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CloneID	OMu10398
Clone ID Related Accession (Same CDS sequence)	XM_006530518.2 , NM_133953.2 , XM_006530518.4 , XM_006530518.3
Accession Version	XM_006530518.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530518.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530581.1
CDS	172..3825
Translation	MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Target ORF information:

RefSeq Version	XM_006530518.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 3 (Sf3b3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530518.4

ORF Insert Sequence:

ATGTTTCTGT ACAACCTGAC CTTACAGCGG GCCACTGGCA TCAGCTTTGC CATCCATGGC 
AACTTCTCTG GAACCAAGCA ACAGGAAATT GTTGTTTCCC GTGGGAAGAT TTTGGAGTTA 
CTACGCCCAG ACCCTAACAC TGGCAAAGTA CATACCCTCC TCACTGTTGA AGTATTTGGT 
GTTATCCGGT CACTTATGGC GTTTAGGCTA ACAGGTGGCA CCAAAGACTA CATTGTAGTT 
GGCAGTGACT CAGGCCGGAT TGTTATCTTG GAATACCAGC CATCAAAGAA TATGTTTGAG 
AAAATTCACC AAGAAACTTT TGGCAAAAGC GGATGTCGCC GTATTGTTCC TGGCCAGTTC 
TTAGCTGTGG ATCCCAAAGG AAGAGCTGTT ATGATTAGTG CAATTGAGAA GCAGAAATTG 
GTATATATCT TGAACAGAGA CGCTGCAGCC CGACTTACCA TTTCCTCTCC CCTGGAGGCC 
CACAAAGCGA ACACTCTGGT GTATCACGTG GTTGGCGTAG ACGTGGGATT TGAGAATCCT 
ATGTTTGCTT GTCTGGAAAT GGATTATGAG GAGGCAGACA ATGATCCAAC AGGGGAAGCA 
GCAGCCAATA CCCAGCAGAC ACTTACTTTC TATGAACTGG ACCTTGGCTT AAATCATGTG 
GTTCGGAAAT ACAGTGAACC CTTGGAGGAA CATGGCAACT TCCTTATTAC AGTTCCTGGA 
GGGTCAGATG GTCCAAGTGG AGTGCTAATC TGCTCTGAGA ATTATATCAC CTACAAGAAC 
TTTGGTGACC AGCCAGACAT CCGATGTCCA ATTCCCAGGA GACGGAATGA CCTGGATGAC 
CCTGAACGAG GAATGATTTT TGTTTGCTCT GCCACCCATA AGACCAAGTC CATGTTCTTC 
TTTCTGGCCC AAACGGAGCA GGGAGATATC TTTAAGATCA CTTTAGAGAC AGATGAAGAT 
ATGGTAACCG AGATCCGCCT CAAGTATTTT GATACTGTGC CTGTTGCTGC TGCTATGTGT 
GTGCTTAAAA CAGGCTTCCT TTTTGTGGCG TCAGAATTTG GAAATCATTA CTTGTATCAA 
ATTGCCCATC TCGGAGATGA TGATGAAGAA CCTGAGTTTT CGTCTGCCAT GCCTCTAGAA 
GAAGGAGACA CATTCTTCTT TCAGCCGAGA CCACTCAAAA ATCTCGTGCT GGTGGATGAA 
CTGGACAGCC TCTCTCCTAT TCTGTTTTGC CAGATAGCTG ATTTGGCCAA TGAAGACACC 
CCACAGCTGT ATGTAGCCTG TGGTAGGGGA CCCCGGTCCT CCCTGAGAGT CTTAAGGCAT 
GGACTTGAGG TGTCGGAAAT GGCTGTTTCT GAGCTCCCTG GTAACCCCAA TGCTGTCTGG 
ACAGTCCGTC GGCACATTGA AGATGAGTTT GATGCCTACA TCATTGTGTC TTTCGTGAAT 
GCCACACTTG TGCTATCTAT CGGAGAGACT GTGGAAGAAG TGACTGACTC TGGCTTCTTG 
GGCACCACTC CAACCTTGTC CTGCTCCTTG TTAGGAGATG ATGCACTGGT GCAGGTGTAT 
CCAGATGGCA TCCGACACAT ACGAGCAGAT AAAAGAGTTA ATGAGTGGAA GACTCCCGGC 
AAGAAAACGA TTGTGAAGTG TGCAGTGAAC CAGCGACAAG TGGTGATCGC CCTGACTGGG 
GGAGAGCTGG TCTATTTCGA GATGGATCCT TCAGGACAGT TGAATGAGTA CACGGAGCGG 
AAAGAGATGT CAGCAGATGT GGTGTGCATG AGTCTGGCCA ACGTGCCCCC TGGAGAACAG 
CGATCTCGGT TCCTGGCTGT GGGGCTGGTG GACAACACTG TCAGAATCAT CTCCCTCGAC 
CCATCAGACT GTTTACAGCC TCTGAGTATG CAGGCTCTCC CAGCCCAGCC TGAGTCTCTA 
TGTATTGTGG AAATGGGTGG GACTGAGAAA CAAGATGAGC TGGGTGAGAG AGGCTCTATT 
GGCTTCCTGT ACCTGAACAT TGGACTACAG AACGGTGTAC TGCTGAGGAC TGTCCTAGAC 
CCTGTTACTG GGGACCTGTC TGACACCCGC ACTAGGTATC TGGGGTCCCG TCCTGTGAAA 
CTCTTCCGGG TCCGGATGCA AGGCCAGGAG GCTGTTCTGG CTATGTCCAG TCGCTCATGG 
CTGAGCTACT CTTACCAGTC TCGCTTCCAT CTCACCCCTC TGTCTTATGA GACCCTGGAA 
TTTGCATCGG GCTTTGCCTC TGAACAGTGT CCCGAGGGCA TTGTTGCCAT CTCCACCAAC 
ACTTTGAGGA TTTTGGCTTT AGAGAAGCTT GGTGCTGTCT TCAATCAAGT AGCCTTCCCA 
TTGCAGTACA CACCCAGGAA GTTTGTCATC CACCCTGAGA GTAACAACCT TATCATCATT 
GAGACGGATC ACAATGCCTA CACTGAGGCC ACAAAAGCTC AGAGAAAGCA GCAGATGGCT 
GAGGAAATGG TAGAAGCAGC AGGGGAGGAT GAGCGGGAGT TGGCTGCAGA AATGGCAGCA 
GCATTCCTCA ATGAAAACCT CCCAGAATCC ATTTTTGGAG CTCCCAAGGC TGGCAATGGG 
CAGTGGGCCT CTGTGATTCG GGTGATGAAC CCTATCCAGG GGAATACACT AGACCTTGTC 
CAGTTGGAGC AAAACGAGGC AGCCTTTAGT GTGGCAGTGT GTAGGTTCTC CAACACTGGT 
GAAGATTGGT ATGTGCTGGT GGGTGTTGCC AAGGACTTGA TACTGAGCCC TCGATCTGTG 
GCAGGGGGCT TTGTGTATAC GTACAAGCTT GTGAACAACG GGGAGAAGTT GGAGTTTCTG 
CACAAGACTC CAGTGGAAGA GGTCCCTGCT GCCATTGCCC CATTCCAGGG GAGGGTACTG 
ATTGGTGTCG GGAAGCTTTT ACGAGTTTAT GACCTGGGGA AGAAGAAGTT ACTTCGAAAG 
TGTGAGAATA AGCATATTGC CAATTACATA TCTGGGATCC AGACAATTGG TCACAGGGTA 
ATTGTGTCTG ATGTCCAAGA AAGTTTCATC TGGGTTCGAT ACAAGCGTAA CGAGAACCAG 
CTCATTATCT TTGCTGATGA CACCTATCCC AGATGGGTCA CCACAGCCAG CCTTCTCGAC 
TATGACACTG TGGCTGGGGC AGACAAGTTT GGCAACATTT GTGTGGTGAG GCTCCCACCT 
AACACCAACG ATGAGGTGGA TGAAGATCCC ACTGGAAACA AAGCCCTGTG GGACCGGGGC 
TTGCTCAATG GGGCTTCACA GAAGGCAGAG GTCATCATGA ACTACCACGT TGGAGAGACC 
GTGCTGTCGC TACAGAAGAC CACACTGATC CCTGGAGGCT CAGAGTCCCT TGTCTATACC 
ACTTTATCTG GAGGAATTGG CATCCTTGTC CCTTTCACGT CTCACGAGGA CCATGACTTC 
TTCCAGCATG TTGAAATGCA CCTGCGGTCT GAGCATCCCC CTCTTTGTGG ACGGGACCAC 
CTGAGCTTTC GTTCTTACTA TTTCCCTGTT AAGAATGTGA TTGATGGAGA CCTTTGTGAG 
CAGTTCAACT CCATGGAACC CAACAAGCAG AAGAACGTCT CTGAGGAGCT GGACCGAACC 
CCACCTGAAG TGTCCAAGAA GCTTGAGGAC ATTAGGACCC GCTATGCCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
BMC developmental biology514(2005 Jul)
McKee AE,Minet E,Stern C,Riahi S,Stiles CD,Silver PA

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Sf3b3 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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