Cacna1e cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Cacna1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu27881 XM_006529105.4
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu76950 XM_017312867.2
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu67260 XM_011247909.3
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu67260 XM_030243926.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu67260 XM_017312578.2
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu67260 XM_030243929.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu67267 XM_011247916.3
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu76948 XM_017312766.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu76949 XM_017312855.2
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu84991 XM_030244154.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu13081 NM_009782.3
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1672.30
$2389.00
OMu27870 XM_017312619.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu27874 XM_017312682.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu27876 XM_017312745.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu27877 XM_017312795.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu27878 XM_017312824.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67260 XM_017312594.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67261 XM_017312613.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67262 XM_017312628.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67263 XM_017312642.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67264 XM_017312660.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67265 XM_017312712.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67266 XM_017312730.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67267 XM_017312848.1
Latest version!
Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu67267 XM_011247916.2 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu76950 XM_017312867.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu76949 XM_017312855.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu27881 XM_006529105.3 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu67260 XM_017312578.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu67260 XM_011247909.2 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu27881
    Clone ID Related Accession (Same CDS sequence) XM_006529105.4 , XM_006529105.3
    Accession Version XM_006529105.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529168.1
    CDS696..6410
    Translation

    Target ORF information:

    RefSeq Version XM_006529105.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529105.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76950
    Clone ID Related Accession (Same CDS sequence) XM_017312867.1 , XM_017312867.2
    Accession Version XM_017312867.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC
    CCCAGTCCCC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168356.1
    CDS698..6535
    Translation

    Target ORF information:

    RefSeq Version XM_017312867.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC
    CCCAGTCCCC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76950
    Clone ID Related Accession (Same CDS sequence) XM_017312867.1 , XM_017312867.2
    Accession Version XM_017312867.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC
    CCCAGTCCCC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168356.1
    CDS697..6534
    Translation

    Target ORF information:

    RefSeq Version XM_017312867.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC
    CCCAGTCCCC AGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27881
    Clone ID Related Accession (Same CDS sequence) XM_006529105.4 , XM_006529105.3
    Accession Version XM_006529105.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529168.1
    CDS697..6411
    Translation

    Target ORF information:

    RefSeq Version XM_006529105.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529105.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_030243926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030099786.1
    CDS670..8070
    Translation

    Target ORF information:

    RefSeq Version XM_030243926.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030243926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_030243929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030099789.1
    CDS93..7493
    Translation

    Target ORF information:

    RefSeq Version XM_030243929.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030243929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_017312578.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168067.1
    CDS598..7998
    Translation

    Target ORF information:

    RefSeq Version XM_017312578.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312578.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_011247909.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246211.1
    CDS522..7922
    Translation

    Target ORF information:

    RefSeq Version XM_011247909.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247909.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67267
    Clone ID Related Accession (Same CDS sequence) XM_011247916.3 , XM_011247916.2 , XM_017312848.1
    Accession Version XM_011247916.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA
    AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG
    GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246218.1
    CDS775..7737
    Translation

    Target ORF information:

    RefSeq Version XM_011247916.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247916.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA
    AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG
    GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76948
    Clone ID Related Accession (Same CDS sequence) XM_017312766.1
    Accession Version XM_017312766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT
    CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT
    ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA
    AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168255.1
    CDS594..7853
    Translation

    Target ORF information:

    RefSeq Version XM_017312766.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT
    CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT
    ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA
    AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76949
    Clone ID Related Accession (Same CDS sequence) XM_017312855.2 , XM_017312855.1
    Accession Version XM_017312855.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168344.1
    CDS695..6856
    Translation

    Target ORF information:

    RefSeq Version XM_017312855.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76949
    Clone ID Related Accession (Same CDS sequence) XM_017312855.2 , XM_017312855.1
    Accession Version XM_017312855.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168344.1
    CDS696..6857
    Translation

    Target ORF information:

    RefSeq Version XM_017312855.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu84991
    Clone ID Related Accession (Same CDS sequence) XM_030244154.1
    Accession Version XM_030244154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGGC ACACCTCTTG CCAGAGCCAG TATCAAGAGC
    ACAAAAGTAG ATGGGGCCTC CTATTTCCGG CACAAGGAAC GGCTTCTGCG CATCTCTATC
    CGCCACATGG TCAAGTCCCA AGTCTTTTAC TGGATCGTCC TGAGTGTTGT GGCTCTTAAT
    ACTGCCTGTG TGGCCATTGT TCATCACAAC CAGCCACAGT GGCTCACTCA CCTCCTCTAC
    TATGCAGAGT TTTTGTTTCT GGGACTCTTC CTGTTGGAGA TGTCCCTGAA GATGTATGGC
    ATGGGGCCAC GCCTCTATTT TCACTCATCC TTCAACTGCT TTGATTTTGG GGTCACAGTG
    GGCAGCATCT TTGAGGTTGT CTGGGCAATC TTCAGACCTG GCACATCTTT TGGAATCAGT
    GTCTTACGAG CTCTCCGGCT CCTAAGGATA TTTAAAATAA CCAAGTATTG GGCATCCCTC
    CGGAATCTGG TTGTCTCCTT GATGAGCTCC ATGAAGTCTA TCATCAGCTT ACTCTTCCTT
    CTCTTCCTCT TCATCGTTGT CTTTGCTCTC CTGGGAATGC AGCTCTTTGG AGGCAGGTTT
    AACTTTAATG ATGGGACTCC TTCAGCTAAT TTTGACACCT TCCCTGCAGC TATCATGACA
    GTGTTCCAGA TCCTGACAGG GGAAGACTGG AATGAGGTGA TGTACAATGG CATTCGCTCC
    CAGGGTGGGG TCAGCTCAGG CATGTGGTCT GCCATCTACT TCATTGTGCT CACCTTGTTT
    GGAAACTACA CGCTGCTAAA TGTATTCTTG GCCATCGCTG TGGACAATCT GGCCAATGCC
    CAGGAACTGA CCAAGGATGA GCAGGAGGAA GAAGAAGCCT TCAACCAGAA ACATGCACTG
    CAGAAGGCCA AGGAGGTCAG CCCGATGTCT GCTCCCAACA TGCCTTCCAT TGAGAGGGAG
    CGGAGGCGCC GGCACCACAT GTCTGTGTGG GAACAACGCA CCAGCCAGCT GAGGAGGCAC
    ATGCAAATGT CTAGCCAAGA AGCCCTCAAC AAAGAGGAGG CCCCGCCAAT GAACCCCCTC
    AATCCACTCA ACCCACTGAG TCCTCTCAAC CCCCTCAATG CTCACCCCAG CCTTTATCGG
    AGGCCCAGGC CCATTGAGGG CCTAGCCCTA GGTCTGGGTC TTGAAAAGTG TGAAGAAGAA
    CGCATCAGTC GTGGGGGCTC CCTCAAGGGG GACATAGGGG GCCTGACTAG CGCCCTGGAC
    AACCAGAGGA GCCCCTTGTC CTTGGGCAAA CGGGAGCCAC CGTGGCTGCC TAGATCCTGT
    CATGGAAACT GTGACCCAAT CCAGCAGGAG GCTGGGGGAG GAGAGACTGT GGTGACTTTT
    GAGGACCGGG CCAGGCACAG GCAGAGTCAG AGGCGAAGCC GGCATCGCCG AGTCAGGACT
    GAGGGCAAGG ACTCTGCCTC TGCATCCCGA AGCAGGTCTG CCAGCCAGGA GCGGAGTCTG
    GATGAAGGGG TGTCCGTTGA GGGGGAGAAG GAACATGAGC CTCACAGCAG CCACAGGAGC
    AAAGAGCCGA CCATCCACGA AGAGGAGAGA ACCCAGGACT TAAGGAGGAC CAACAGTCTG
    ATGGTCCCCA GGGGCTCTGG ACTGGTAGGG GCTCTTGATG AAGCAGAAAC ACCTCTAGTC
    CAGCCCCAAC CTGAGCTGGA AGTGGGGAAG GATGCAGCTC TGACAGAGCA GGAGGCTGAA
    GGCAGCAGTG AACAAGCCCT TCTTGGGGAC GTACAGCTGG ATGTGGGCCG GGGCATTAGC
    CAGAGTGAAC CGGATCTCTC CTGCATGACA GCCAACATGG ACAAGGCTAC CACGGAGAGC
    ACCAGTGTCA CGGTCGCCAT CCCTGATGTG GACCCCTTGG TGGACTCGAC AGTGGTGAAC
    ATTAGCAACA AGACGGATGG AGAAGCCAGT CCCTTGAAGG AGGCAGAGAC TAAAGAGGAA
    GAAGAAGAGG TAGAGAAGAA GAAGAAGCAG AAGAAGGAGA AGCGAGAGAC TGGAAAAGCC
    ATGGTGCCCC ATAGCTCTAT GTTCATCTTC AGCACCACTA ACCCGATCCG CAGGGCCTGC
    CACTACATTG TGAACCTACG CTACTTTGAA ATGTGCATTC TCTTGGTGAT TGCGGCCAGC
    AGCATTGCCC TGGCAGCCGA GGACCCTGTA CTAACTAACT CGGAGCGTAA CAAAGTCCTG
    AGATATTTCG ACTATGTTTT CACAGGAGTG TTTACCTTTG AGATGGTTAT AAAGATGATA
    GACCAAGGCT TGATCCTGCA GGATGGATCA TACTTCAGAG ACCTCTGGAA CATCCTGGAC
    TTTGTAGTAG TGGTTGGTGC ATTGGTAGCA TTTGCTCTGG CGAACGCTTT GGGAACCAAC
    AAGGGGAGGG ATATCAAGAC TATCAAGTCT CTGAGGGTAC TTCGAGTTCT GCGGCCACTG
    AAAACCATCA AGCGCTTGCC CAAGCTGAAG GCTGTGTTTG ACTGCGTGGT GACCTCCTTG
    AAGAATGTCT TCAACATACT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCCGTCATC
    GCTGTTCAGC TATTCAAAGG AAAGTTCTTT TATTGCACTG ACAGTTCCAA GGACACAGAG
    AAGGAGTGCA TAGGCAACTA TGTAGACCAC GAGAAAAACA AGATGGAGGT GAAGGGCCGG
    GAATGGAAGC GTCATGAATT CCACTATGAC AACATCATCT GGGCCCTGCT TACCCTCTTC
    ACAGTCTCTA CAGGGGAAGG ATGGCCTCAG GTTCTGCAGC ACTCTGTAGA TGTGACAGAG
    GAGGACAGAG GCCCAAGTCG CAGCAACCGC ATGGAGATGT CCATCTTTTA TGTGGTCTAC
    TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTCGTGG CTCTCATTAT CATCACCTTC
    CAGGAGCAAG GGGACAAGAT GATGGAGGAG TGTAGCCTGG AGAAGAATGA GCGGGCATGC
    ATCGACTTCG CCATTAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC
    TTCCAGTACC GTGTGTGGCA CTTTGTAGTC TCCCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCCT TGAACACTGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC TTGCACCTAT
    GAGCTCGCCT TAAAGTACCT GAACATCGCC TTCACTATGG TGTTTTCCCT GGAGTGTGTC
    CTAAAGGTCA TTGCTTTCGG CTTCCTGAAC TATTTCCGAG ATACCTGGAA CATCTTTGAC
    TTCATCACGG TGATCGGCAG TATCACAGAA ATTATCCTGA CAGATAGCAA GCTGGTGAAC
    ACCAGTGGCT TCAATATGAG CTTTCTGAAG CTCTTCCGAG CTGCACGGCT CATAAAGCTT
    CTGAGACAGG GCTACACCAT CCGCATCTTA CTGTGGACCT TCGTGCAGTC CTTTAAGGCT
    CTTCCCTACG TCTGCCTTCT CATTGCCATG CTTTTCTTCA TCTACGCCAT CATTGGGATG
    CAGGTGTTTG GAAACATAAA GTTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC
    CGGAGTTTCT TTGGATCCCT CATGCTGCTT TTCAGGAGTG CCACGGGGGA GGCTTGGCAG
    GAGATTATGC TGTCCTGTCT CGGTGAGAAG GGTTGTGAGC CTGACACCAC TGCACCCTCA
    GGGCAGAATG AGAGTGAGCG CTGTGGCACT GACCTGGCCT ACGTTTATTT TGTCTCCTTC
    ATCTTCTTCT GCTCCTTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGGCCTCACC ACTTAGATGA GTTTGTCCGT
    GTCTGGGCAG AATATGACAG AGCAGCATGT GGCCGCATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCC
    TATAAGAGGC TGGTCCTGAT GAACATGCCA GTGGCTGAGG ACATGACAGT CCACTTCACC
    TCCACACTTA TGGCTCTGAT CCGGACAGCT CTGGACATTA AAATCGCCAA AGGTGGTGCA
    GACAGACAGC AGCTAGACTC GGAGCTGCAA AAAGAGACCC TGGCCATTTG GCCTCACCTG
    TCCCAAAAGA TGTTGGATCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC
    AAGATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT AAAGAAACAG
    AGACAGCAGC TGGAAGAACA GAAAAATGCA CCCATGTTCC AGCGCATGGA GCCCTCATCA
    CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCTT ATCTCCAGCA GGACCCTGTT
    TCTGGCCTGA GTGGTCGGAG TGGATACCCT TCAATGAGTC CACTCTCCCC TCAAGAAATA
    TTCCAGTTGG CTTGTATGGA CCCAGCGGAT GATGGACAAT TCCAGGAACA ACAATCTCTG
    GTGAATGCAC AAGAGCCAGA GGTTAGTGAA TTAAAAAGTG TGCAGTCCTC TAACCATGGC
    ATCTACCTTC CTCCGGACAC CCAGGAGCAT GCGGGATCTG GGAGGGCATC CTCTATGCCA
    CGTCTGACTA TGGATCCCCA GGTGGTAACA GACCCTAGCT CTATGCGACG TTCATTTTCT
    ACAATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AGTTCTCCAT GGAACGCAGC
    AGTGAAAACA CTTACAAGTC GCGGCGCAGA AGTTACCATT CCTCCCTGAG GCTGTCAGCA
    CACCGCCTGA ATTCTGACTC TGGTCACAAG TCTGACACTC ACCGATCAGG AGGCAGGGAG
    AGAGGCCGAT CCAAAGAACG AAAGCACCTC CTCTCTCCAG ATGTCTCCCG CTGCAATTCT
    GAAGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAAC GTCGCCAGTC CAGGTCACCC
    AGTGAAGGAA GGTCACAGAC CCCCAATAGA CAGGGTACCG GTTCCCTAAG TGAGAGCTCC
    ATCCCCTCTA TTTCCGACAC CAGCACCCCA AGACGAAGCC GTCGCCAGCT CCCACCTGTG
    CCACCAAAGC CTCGTCCCCT CCTCTCCTAC AGCTCCCTGA TGAGACACAC TGGCGGCATC
    TCTCCACCTC CTGATGGAAG TGAGGGTGGA TCCCCACTGG CCTCTCAAGC TCTGGAGAGC
    AACAGCGCTT GCCTGACCGA GTCTTCCAAC TCCTTGCACC CCCAGCAGGG CCAGCATCCT
    TCCCCACAGC ACTACATCTC CGAGCCCTAT CTGGCCCTCC ATGAAGACTC CCACGCCTCA
    GACTGTGGCG AGGAGGAGAC ACTCACCTTT GAGGCAGCCG TAGCCACTAG CTTGGGCCGG
    TCCAATACCA TTGGCTCTGC GCCACCCCTA CGGCACAGCT GGCAGATGCC TAATGGGCAC
    TATCGGCGGC GGAGGCGTGG GGGGCCTGGG CCTGGCATGA TGTGTGGAGC TGTCAGTGAC
    CTCCTGAGTG ACACGGAAGA AGACGATAAG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100014.1
    CDS1744..8559
    Translation

    Target ORF information:

    RefSeq Version XM_030244154.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030244154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGGC ACACCTCTTG CCAGAGCCAG TATCAAGAGC
    ACAAAAGTAG ATGGGGCCTC CTATTTCCGG CACAAGGAAC GGCTTCTGCG CATCTCTATC
    CGCCACATGG TCAAGTCCCA AGTCTTTTAC TGGATCGTCC TGAGTGTTGT GGCTCTTAAT
    ACTGCCTGTG TGGCCATTGT TCATCACAAC CAGCCACAGT GGCTCACTCA CCTCCTCTAC
    TATGCAGAGT TTTTGTTTCT GGGACTCTTC CTGTTGGAGA TGTCCCTGAA GATGTATGGC
    ATGGGGCCAC GCCTCTATTT TCACTCATCC TTCAACTGCT TTGATTTTGG GGTCACAGTG
    GGCAGCATCT TTGAGGTTGT CTGGGCAATC TTCAGACCTG GCACATCTTT TGGAATCAGT
    GTCTTACGAG CTCTCCGGCT CCTAAGGATA TTTAAAATAA CCAAGTATTG GGCATCCCTC
    CGGAATCTGG TTGTCTCCTT GATGAGCTCC ATGAAGTCTA TCATCAGCTT ACTCTTCCTT
    CTCTTCCTCT TCATCGTTGT CTTTGCTCTC CTGGGAATGC AGCTCTTTGG AGGCAGGTTT
    AACTTTAATG ATGGGACTCC TTCAGCTAAT TTTGACACCT TCCCTGCAGC TATCATGACA
    GTGTTCCAGA TCCTGACAGG GGAAGACTGG AATGAGGTGA TGTACAATGG CATTCGCTCC
    CAGGGTGGGG TCAGCTCAGG CATGTGGTCT GCCATCTACT TCATTGTGCT CACCTTGTTT
    GGAAACTACA CGCTGCTAAA TGTATTCTTG GCCATCGCTG TGGACAATCT GGCCAATGCC
    CAGGAACTGA CCAAGGATGA GCAGGAGGAA GAAGAAGCCT TCAACCAGAA ACATGCACTG
    CAGAAGGCCA AGGAGGTCAG CCCGATGTCT GCTCCCAACA TGCCTTCCAT TGAGAGGGAG
    CGGAGGCGCC GGCACCACAT GTCTGTGTGG GAACAACGCA CCAGCCAGCT GAGGAGGCAC
    ATGCAAATGT CTAGCCAAGA AGCCCTCAAC AAAGAGGAGG CCCCGCCAAT GAACCCCCTC
    AATCCACTCA ACCCACTGAG TCCTCTCAAC CCCCTCAATG CTCACCCCAG CCTTTATCGG
    AGGCCCAGGC CCATTGAGGG CCTAGCCCTA GGTCTGGGTC TTGAAAAGTG TGAAGAAGAA
    CGCATCAGTC GTGGGGGCTC CCTCAAGGGG GACATAGGGG GCCTGACTAG CGCCCTGGAC
    AACCAGAGGA GCCCCTTGTC CTTGGGCAAA CGGGAGCCAC CGTGGCTGCC TAGATCCTGT
    CATGGAAACT GTGACCCAAT CCAGCAGGAG GCTGGGGGAG GAGAGACTGT GGTGACTTTT
    GAGGACCGGG CCAGGCACAG GCAGAGTCAG AGGCGAAGCC GGCATCGCCG AGTCAGGACT
    GAGGGCAAGG ACTCTGCCTC TGCATCCCGA AGCAGGTCTG CCAGCCAGGA GCGGAGTCTG
    GATGAAGGGG TGTCCGTTGA GGGGGAGAAG GAACATGAGC CTCACAGCAG CCACAGGAGC
    AAAGAGCCGA CCATCCACGA AGAGGAGAGA ACCCAGGACT TAAGGAGGAC CAACAGTCTG
    ATGGTCCCCA GGGGCTCTGG ACTGGTAGGG GCTCTTGATG AAGCAGAAAC ACCTCTAGTC
    CAGCCCCAAC CTGAGCTGGA AGTGGGGAAG GATGCAGCTC TGACAGAGCA GGAGGCTGAA
    GGCAGCAGTG AACAAGCCCT TCTTGGGGAC GTACAGCTGG ATGTGGGCCG GGGCATTAGC
    CAGAGTGAAC CGGATCTCTC CTGCATGACA GCCAACATGG ACAAGGCTAC CACGGAGAGC
    ACCAGTGTCA CGGTCGCCAT CCCTGATGTG GACCCCTTGG TGGACTCGAC AGTGGTGAAC
    ATTAGCAACA AGACGGATGG AGAAGCCAGT CCCTTGAAGG AGGCAGAGAC TAAAGAGGAA
    GAAGAAGAGG TAGAGAAGAA GAAGAAGCAG AAGAAGGAGA AGCGAGAGAC TGGAAAAGCC
    ATGGTGCCCC ATAGCTCTAT GTTCATCTTC AGCACCACTA ACCCGATCCG CAGGGCCTGC
    CACTACATTG TGAACCTACG CTACTTTGAA ATGTGCATTC TCTTGGTGAT TGCGGCCAGC
    AGCATTGCCC TGGCAGCCGA GGACCCTGTA CTAACTAACT CGGAGCGTAA CAAAGTCCTG
    AGATATTTCG ACTATGTTTT CACAGGAGTG TTTACCTTTG AGATGGTTAT AAAGATGATA
    GACCAAGGCT TGATCCTGCA GGATGGATCA TACTTCAGAG ACCTCTGGAA CATCCTGGAC
    TTTGTAGTAG TGGTTGGTGC ATTGGTAGCA TTTGCTCTGG CGAACGCTTT GGGAACCAAC
    AAGGGGAGGG ATATCAAGAC TATCAAGTCT CTGAGGGTAC TTCGAGTTCT GCGGCCACTG
    AAAACCATCA AGCGCTTGCC CAAGCTGAAG GCTGTGTTTG ACTGCGTGGT GACCTCCTTG
    AAGAATGTCT TCAACATACT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCCGTCATC
    GCTGTTCAGC TATTCAAAGG AAAGTTCTTT TATTGCACTG ACAGTTCCAA GGACACAGAG
    AAGGAGTGCA TAGGCAACTA TGTAGACCAC GAGAAAAACA AGATGGAGGT GAAGGGCCGG
    GAATGGAAGC GTCATGAATT CCACTATGAC AACATCATCT GGGCCCTGCT TACCCTCTTC
    ACAGTCTCTA CAGGGGAAGG ATGGCCTCAG GTTCTGCAGC ACTCTGTAGA TGTGACAGAG
    GAGGACAGAG GCCCAAGTCG CAGCAACCGC ATGGAGATGT CCATCTTTTA TGTGGTCTAC
    TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTCGTGG CTCTCATTAT CATCACCTTC
    CAGGAGCAAG GGGACAAGAT GATGGAGGAG TGTAGCCTGG AGAAGAATGA GCGGGCATGC
    ATCGACTTCG CCATTAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC
    TTCCAGTACC GTGTGTGGCA CTTTGTAGTC TCCCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCCT TGAACACTGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC TTGCACCTAT
    GAGCTCGCCT TAAAGTACCT GAACATCGCC TTCACTATGG TGTTTTCCCT GGAGTGTGTC
    CTAAAGGTCA TTGCTTTCGG CTTCCTGAAC TATTTCCGAG ATACCTGGAA CATCTTTGAC
    TTCATCACGG TGATCGGCAG TATCACAGAA ATTATCCTGA CAGATAGCAA GCTGGTGAAC
    ACCAGTGGCT TCAATATGAG CTTTCTGAAG CTCTTCCGAG CTGCACGGCT CATAAAGCTT
    CTGAGACAGG GCTACACCAT CCGCATCTTA CTGTGGACCT TCGTGCAGTC CTTTAAGGCT
    CTTCCCTACG TCTGCCTTCT CATTGCCATG CTTTTCTTCA TCTACGCCAT CATTGGGATG
    CAGGTGTTTG GAAACATAAA GTTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC
    CGGAGTTTCT TTGGATCCCT CATGCTGCTT TTCAGGAGTG CCACGGGGGA GGCTTGGCAG
    GAGATTATGC TGTCCTGTCT CGGTGAGAAG GGTTGTGAGC CTGACACCAC TGCACCCTCA
    GGGCAGAATG AGAGTGAGCG CTGTGGCACT GACCTGGCCT ACGTTTATTT TGTCTCCTTC
    ATCTTCTTCT GCTCCTTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGGCCTCACC ACTTAGATGA GTTTGTCCGT
    GTCTGGGCAG AATATGACAG AGCAGCATGT GGCCGCATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCC
    TATAAGAGGC TGGTCCTGAT GAACATGCCA GTGGCTGAGG ACATGACAGT CCACTTCACC
    TCCACACTTA TGGCTCTGAT CCGGACAGCT CTGGACATTA AAATCGCCAA AGGTGGTGCA
    GACAGACAGC AGCTAGACTC GGAGCTGCAA AAAGAGACCC TGGCCATTTG GCCTCACCTG
    TCCCAAAAGA TGTTGGATCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC
    AAGATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT AAAGAAACAG
    AGACAGCAGC TGGAAGAACA GAAAAATGCA CCCATGTTCC AGCGCATGGA GCCCTCATCA
    CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCTT ATCTCCAGCA GGACCCTGTT
    TCTGGCCTGA GTGGTCGGAG TGGATACCCT TCAATGAGTC CACTCTCCCC TCAAGAAATA
    TTCCAGTTGG CTTGTATGGA CCCAGCGGAT GATGGACAAT TCCAGGAACA ACAATCTCTG
    GTGAATGCAC AAGAGCCAGA GGTTAGTGAA TTAAAAAGTG TGCAGTCCTC TAACCATGGC
    ATCTACCTTC CTCCGGACAC CCAGGAGCAT GCGGGATCTG GGAGGGCATC CTCTATGCCA
    CGTCTGACTA TGGATCCCCA GGTGGTAACA GACCCTAGCT CTATGCGACG TTCATTTTCT
    ACAATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AGTTCTCCAT GGAACGCAGC
    AGTGAAAACA CTTACAAGTC GCGGCGCAGA AGTTACCATT CCTCCCTGAG GCTGTCAGCA
    CACCGCCTGA ATTCTGACTC TGGTCACAAG TCTGACACTC ACCGATCAGG AGGCAGGGAG
    AGAGGCCGAT CCAAAGAACG AAAGCACCTC CTCTCTCCAG ATGTCTCCCG CTGCAATTCT
    GAAGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAAC GTCGCCAGTC CAGGTCACCC
    AGTGAAGGAA GGTCACAGAC CCCCAATAGA CAGGGTACCG GTTCCCTAAG TGAGAGCTCC
    ATCCCCTCTA TTTCCGACAC CAGCACCCCA AGACGAAGCC GTCGCCAGCT CCCACCTGTG
    CCACCAAAGC CTCGTCCCCT CCTCTCCTAC AGCTCCCTGA TGAGACACAC TGGCGGCATC
    TCTCCACCTC CTGATGGAAG TGAGGGTGGA TCCCCACTGG CCTCTCAAGC TCTGGAGAGC
    AACAGCGCTT GCCTGACCGA GTCTTCCAAC TCCTTGCACC CCCAGCAGGG CCAGCATCCT
    TCCCCACAGC ACTACATCTC CGAGCCCTAT CTGGCCCTCC ATGAAGACTC CCACGCCTCA
    GACTGTGGCG AGGAGGAGAC ACTCACCTTT GAGGCAGCCG TAGCCACTAG CTTGGGCCGG
    TCCAATACCA TTGGCTCTGC GCCACCCCTA CGGCACAGCT GGCAGATGCC TAATGGGCAC
    TATCGGCGGC GGAGGCGTGG GGGGCCTGGG CCTGGCATGA TGTGTGGAGC TGTCAGTGAC
    CTCCTGAGTG ACACGGAAGA AGACGATAAG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13081
    Clone ID Related Accession (Same CDS sequence) NM_009782.3
    Accession Version NM_009782.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC101727.8, AC099707.14 and AC122040.4. On Nov 15, 2006 this sequence version replaced NM_009782.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG GTAACAGACC CTAGCTCTAT GCGACGTTCA
    TTTTCTACAA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAGTT CTCCATGGAA
    CGCAGCAGTG AAAACACTTA CAAGTCGCGG CGCAGAAGTT ACCATTCCTC CCTGAGGCTG
    TCAGCACACC GCCTGAATTC TGACTCTGGT CACAAGTCTG ACACTCACCG ATCAGGAGGC
    AGGGAGAGAG GCCGATCCAA AGAACGAAAG CACCTCCTCT CTCCAGATGT CTCCCGCTGC
    AATTCTGAAG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAACGTCG CCAGTCCAGG
    TCACCCAGTG AAGGAAGGTC ACAGACCCCC AATAGACAGG GTACCGGTTC CCTAAGTGAG
    AGCTCCATCC CCTCTATTTC CGACACCAGC ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA
    CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC TCCTACAGCT CCCTGATGAG ACACACTGGC
    GGCATCTCTC CACCTCCTGA TGGAAGTGAG GGTGGATCCC CACTGGCCTC TCAAGCTCTG
    GAGAGCAACA GCGCTTGCCT GACCGAGTCT TCCAACTCCT TGCACCCCCA GCAGGGCCAG
    CATCCTTCCC CACAGCACTA CATCTCCGAG CCCTATCTGG CCCTCCATGA AGACTCCCAC
    GCCTCAGACT GTGGCGAGGA GGAGACACTC ACCTTTGAGG CAGCCGTAGC CACTAGCTTG
    GGCCGGTCCA ATACCATTGG CTCTGCGCCA CCCCTACGGC ACAGCTGGCA GATGCCTAAT
    GGGCACTATC GGCGGCGGAG GCGTGGGGGG CCTGGGCCTG GCATGATGTG TGGAGCTGTC
    AGTGACCTCC TGAGTGACAC GGAAGAAGAC GATAAGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_033912.2
    CDS2..6823
    Translation

    Target ORF information:

    RefSeq Version NM_009782.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_009782.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG GTAACAGACC CTAGCTCTAT GCGACGTTCA
    TTTTCTACAA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAGTT CTCCATGGAA
    CGCAGCAGTG AAAACACTTA CAAGTCGCGG CGCAGAAGTT ACCATTCCTC CCTGAGGCTG
    TCAGCACACC GCCTGAATTC TGACTCTGGT CACAAGTCTG ACACTCACCG ATCAGGAGGC
    AGGGAGAGAG GCCGATCCAA AGAACGAAAG CACCTCCTCT CTCCAGATGT CTCCCGCTGC
    AATTCTGAAG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAACGTCG CCAGTCCAGG
    TCACCCAGTG AAGGAAGGTC ACAGACCCCC AATAGACAGG GTACCGGTTC CCTAAGTGAG
    AGCTCCATCC CCTCTATTTC CGACACCAGC ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA
    CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC TCCTACAGCT CCCTGATGAG ACACACTGGC
    GGCATCTCTC CACCTCCTGA TGGAAGTGAG GGTGGATCCC CACTGGCCTC TCAAGCTCTG
    GAGAGCAACA GCGCTTGCCT GACCGAGTCT TCCAACTCCT TGCACCCCCA GCAGGGCCAG
    CATCCTTCCC CACAGCACTA CATCTCCGAG CCCTATCTGG CCCTCCATGA AGACTCCCAC
    GCCTCAGACT GTGGCGAGGA GGAGACACTC ACCTTTGAGG CAGCCGTAGC CACTAGCTTG
    GGCCGGTCCA ATACCATTGG CTCTGCGCCA CCCCTACGGC ACAGCTGGCA GATGCCTAAT
    GGGCACTATC GGCGGCGGAG GCGTGGGGGG CCTGGGCCTG GCATGATGTG TGGAGCTGTC
    AGTGACCTCC TGAGTGACAC GGAAGAAGAC GATAAGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27870
    Clone ID Related Accession (Same CDS sequence) XM_017312619.1
    Accession Version XM_017312619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC
    CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT
    AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC
    CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168108.1
    CDS594..7982
    Translation

    Target ORF information:

    RefSeq Version XM_017312619.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC
    CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT
    AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC
    CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27874
    Clone ID Related Accession (Same CDS sequence) XM_017312682.1
    Accession Version XM_017312682.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGA GCCAGAGGTT
    AGTGAATTAA AAAGTGTGCA GTCCTCTAAC CATGGCATCT ACCTTCCTCC GGACACCCAG
    GAGCATGCGG GATCTGGGAG GGCATCCTCT ATGCCACGTC TGACTATGGA TCCCCAGGTG
    GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT
    TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG
    CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT
    CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG
    CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC
    TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC
    AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC
    ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC
    TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG
    GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT
    TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG
    CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC
    ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA
    CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG
    CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC
    GATAAGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168171.1
    CDS594..7925
    Translation

    Target ORF information:

    RefSeq Version XM_017312682.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312682.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGA GCCAGAGGTT
    AGTGAATTAA AAAGTGTGCA GTCCTCTAAC CATGGCATCT ACCTTCCTCC GGACACCCAG
    GAGCATGCGG GATCTGGGAG GGCATCCTCT ATGCCACGTC TGACTATGGA TCCCCAGGTG
    GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT
    TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG
    CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT
    CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG
    CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC
    TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC
    AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC
    ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC
    TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG
    GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT
    TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG
    CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC
    ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA
    CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG
    CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC
    GATAAGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27876
    Clone ID Related Accession (Same CDS sequence) XM_017312745.1
    Accession Version XM_017312745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT
    CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT
    ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA
    AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168234.1
    CDS594..7853
    Translation

    Target ORF information:

    RefSeq Version XM_017312745.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT
    CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT
    ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA
    AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27877
    Clone ID Related Accession (Same CDS sequence) XM_017312795.1
    Accession Version XM_017312795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168284.1
    CDS594..7796
    Translation

    Target ORF information:

    RefSeq Version XM_017312795.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27878
    Clone ID Related Accession (Same CDS sequence) XM_017312824.1
    Accession Version XM_017312824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGCGG CAGGATCCCG TATAAGGATA TGTACAAATT AGTGCGGGTG
    ATCTCGCCTC CTCTGGGCCT GGGAGAGAAC TGCCCCTACA GGGTGGCTTG CAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168313.1
    CDS594..7796
    Translation

    Target ORF information:

    RefSeq Version XM_017312824.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGCGG CAGGATCCCG TATAAGGATA TGTACAAATT AGTGCGGGTG
    ATCTCGCCTC CTCTGGGCCT GGGAGAGAAC TGCCCCTACA GGGTGGCTTG CAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_017312578.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168067.1
    CDS597..7997
    Translation

    Target ORF information:

    RefSeq Version XM_017312578.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_017312594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168083.1
    CDS36..7436
    Translation

    Target ORF information:

    RefSeq Version XM_017312594.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67260
    Clone ID Related Accession (Same CDS sequence) XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
    Accession Version XM_011247909.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246211.1
    CDS538..7938
    Translation

    Target ORF information:

    RefSeq Version XM_011247909.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67261
    Clone ID Related Accession (Same CDS sequence) XM_017312613.1
    Accession Version XM_017312613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168102.1
    CDS594..7994
    Translation

    Target ORF information:

    RefSeq Version XM_017312613.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG
    CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG
    GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT
    CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG
    CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC
    AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT
    GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA
    GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC
    CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA
    CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC
    GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT
    CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT
    GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG
    ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC
    ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG
    GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC
    TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG
    CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG
    GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67262
    Clone ID Related Accession (Same CDS sequence) XM_017312628.1
    Accession Version XM_017312628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG
    TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG
    AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC
    TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC
    TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC
    CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT
    GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT
    CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC
    CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC
    AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG
    GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT
    CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA
    GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG
    ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC
    GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG
    AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA
    GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC
    TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC
    ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG
    CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG
    AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC
    ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC
    AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC
    CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCACCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCCTATAAGA GGCTGGTCCT GATGAACATG
    CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG
    CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT
    GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC
    CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG
    GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC
    CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT
    AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC
    CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168117.1
    CDS594..7982
    Translation

    Target ORF information:

    RefSeq Version XM_017312628.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG
    TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG
    AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC
    TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC
    TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC
    CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT
    GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT
    CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC
    CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC
    AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG
    GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT
    CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA
    GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG
    ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC
    GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG
    AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA
    GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC
    TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC
    ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG
    CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG
    AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC
    ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC
    AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC
    CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCACCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCCTATAAGA GGCTGGTCCT GATGAACATG
    CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG
    CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT
    GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC
    CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG
    GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC
    CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT
    AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC
    CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67263
    Clone ID Related Accession (Same CDS sequence) XM_017312642.1
    Accession Version XM_017312642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG
    TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG
    AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC
    TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC
    TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC
    CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT
    GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT
    CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC
    CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC
    AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG
    GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT
    CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA
    GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG
    ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC
    GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG
    AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA
    GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC
    TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC
    ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG
    CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG
    AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC
    ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC
    AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC
    CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA
    TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG
    GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGCTGGTCCT GATGAACATG
    CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG
    CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT
    GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC
    CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG
    GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC
    CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT
    AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC
    CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168131.1
    CDS594..7982
    Translation

    Target ORF information:

    RefSeq Version XM_017312642.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG
    TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG
    AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC
    TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC
    TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC
    CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT
    GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT
    CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC
    CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC
    AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG
    GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT
    CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA
    GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG
    ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC
    GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG
    AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA
    GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC
    TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC
    ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG
    CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG
    AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC
    ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC
    AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC
    CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA
    TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG
    GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGCTGGTCCT GATGAACATG
    CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG
    CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT
    GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC
    CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG
    GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC
    CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT
    AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC
    CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67264
    Clone ID Related Accession (Same CDS sequence) XM_017312660.1
    Accession Version XM_017312660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GAATGCACAA
    GAGCCAGAGG TTAGTGAATT AAAAAGTGTG CAGTCCTCTA ACCATGGCAT CTACCTTCCT
    CCGGACACCC AGGAGCATGC GGGATCTGGG AGGGCATCCT CTATGCCACG TCTGACTATG
    GATCCCCAGG TGGTAACAGA CCCTAGCTCT ATGCGACGTT CATTTTCTAC AATTCGGGAT
    AAGCGTTCAA ATTCCTCCTG GTTGGAGGAG TTCTCCATGG AACGCAGCAG TGAAAACACT
    TACAAGTCGC GGCGCAGAAG TTACCATTCC TCCCTGAGGC TGTCAGCACA CCGCCTGAAT
    TCTGACTCTG GTCACAAGTC TGACACTCAC CGATCAGGAG GCAGGGAGAG AGGCCGATCC
    AAAGAACGAA AGCACCTCCT CTCTCCAGAT GTCTCCCGCT GCAATTCTGA AGAGCGAGGG
    ACCCAGGCCG ACTGGGAGTC CCCAGAACGT CGCCAGTCCA GGTCACCCAG TGAAGGAAGG
    TCACAGACCC CCAATAGACA GGGTACCGGT TCCCTAAGTG AGAGCTCCAT CCCCTCTATT
    TCCGACACCA GCACCCCAAG ACGAAGCCGT CGCCAGCTCC CACCTGTGCC ACCAAAGCCT
    CGTCCCCTCC TCTCCTACAG CTCCCTGATG AGACACACTG GCGGCATCTC TCCACCTCCT
    GATGGAAGTG AGGGTGGATC CCCACTGGCC TCTCAAGCTC TGGAGAGCAA CAGCGCTTGC
    CTGACCGAGT CTTCCAACTC CTTGCACCCC CAGCAGGGCC AGCATCCTTC CCCACAGCAC
    TACATCTCCG AGCCCTATCT GGCCCTCCAT GAAGACTCCC ACGCCTCAGA CTGTGGCGAG
    GAGGAGACAC TCACCTTTGA GGCAGCCGTA GCCACTAGCT TGGGCCGGTC CAATACCATT
    GGCTCTGCGC CACCCCTACG GCACAGCTGG CAGATGCCTA ATGGGCACTA TCGGCGGCGG
    AGGCGTGGGG GGCCTGGGCC TGGCATGATG TGTGGAGCTG TCAGTGACCT CCTGAGTGAC
    ACGGAAGAAG ACGATAAGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168149.1
    CDS594..7937
    Translation

    Target ORF information:

    RefSeq Version XM_017312660.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG
    CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT
    GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC
    CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT
    GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC
    TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC
    ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA
    CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG
    GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG
    GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA
    GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT
    AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG
    AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG
    GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC
    TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG
    ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG
    GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT
    ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG
    CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC
    AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA
    TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA
    GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT
    CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA
    GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC
    CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC
    CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG
    GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC
    ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT
    GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG
    AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA
    AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT
    GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG
    ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC
    AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC
    TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC
    TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA
    AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT
    GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG
    TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG
    AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC
    TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA
    TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG
    GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG
    GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG
    CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG
    TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA
    GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG
    GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG
    ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT
    GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT
    TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GAATGCACAA
    GAGCCAGAGG TTAGTGAATT AAAAAGTGTG CAGTCCTCTA ACCATGGCAT CTACCTTCCT
    CCGGACACCC AGGAGCATGC GGGATCTGGG AGGGCATCCT CTATGCCACG TCTGACTATG
    GATCCCCAGG TGGTAACAGA CCCTAGCTCT ATGCGACGTT CATTTTCTAC AATTCGGGAT
    AAGCGTTCAA ATTCCTCCTG GTTGGAGGAG TTCTCCATGG AACGCAGCAG TGAAAACACT
    TACAAGTCGC GGCGCAGAAG TTACCATTCC TCCCTGAGGC TGTCAGCACA CCGCCTGAAT
    TCTGACTCTG GTCACAAGTC TGACACTCAC CGATCAGGAG GCAGGGAGAG AGGCCGATCC
    AAAGAACGAA AGCACCTCCT CTCTCCAGAT GTCTCCCGCT GCAATTCTGA AGAGCGAGGG
    ACCCAGGCCG ACTGGGAGTC CCCAGAACGT CGCCAGTCCA GGTCACCCAG TGAAGGAAGG
    TCACAGACCC CCAATAGACA GGGTACCGGT TCCCTAAGTG AGAGCTCCAT CCCCTCTATT
    TCCGACACCA GCACCCCAAG ACGAAGCCGT CGCCAGCTCC CACCTGTGCC ACCAAAGCCT
    CGTCCCCTCC TCTCCTACAG CTCCCTGATG AGACACACTG GCGGCATCTC TCCACCTCCT
    GATGGAAGTG AGGGTGGATC CCCACTGGCC TCTCAAGCTC TGGAGAGCAA CAGCGCTTGC
    CTGACCGAGT CTTCCAACTC CTTGCACCCC CAGCAGGGCC AGCATCCTTC CCCACAGCAC
    TACATCTCCG AGCCCTATCT GGCCCTCCAT GAAGACTCCC ACGCCTCAGA CTGTGGCGAG
    GAGGAGACAC TCACCTTTGA GGCAGCCGTA GCCACTAGCT TGGGCCGGTC CAATACCATT
    GGCTCTGCGC CACCCCTACG GCACAGCTGG CAGATGCCTA ATGGGCACTA TCGGCGGCGG
    AGGCGTGGGG GGCCTGGGCC TGGCATGATG TGTGGAGCTG TCAGTGACCT CCTGAGTGAC
    ACGGAAGAAG ACGATAAGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67265
    Clone ID Related Accession (Same CDS sequence) XM_017312712.1
    Accession Version XM_017312712.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGGCAC ACCTCTTGCC
    AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT GGGGCCTCCT ATTTCCGGCA CAAGGAACGG
    CTTCTGCGCA TCTCTATCCG CCACATGGTC AAGTCCCAAG TCTTTTACTG GATCGTCCTG
    AGTGTTGTGG CTCTTAATAC TGCCTGTGTG GCCATTGTTC ATCACAACCA GCCACAGTGG
    CTCACTCACC TCCTCTACTA TGCAGAGTTT TTGTTTCTGG GACTCTTCCT GTTGGAGATG
    TCCCTGAAGA TGTATGGCAT GGGGCCACGC CTCTATTTTC ACTCATCCTT CAACTGCTTT
    GATTTTGGGG TCACAGTGGG CAGCATCTTT GAGGTTGTCT GGGCAATCTT CAGACCTGGC
    ACATCTTTTG GAATCAGTGT CTTACGAGCT CTCCGGCTCC TAAGGATATT TAAAATAACC
    AAGTATTGGG CATCCCTCCG GAATCTGGTT GTCTCCTTGA TGAGCTCCAT GAAGTCTATC
    ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC ATCGTTGTCT TTGCTCTCCT GGGAATGCAG
    CTCTTTGGAG GCAGGTTTAA CTTTAATGAT GGGACTCCTT CAGCTAATTT TGACACCTTC
    CCTGCAGCTA TCATGACAGT GTTCCAGATC CTGACAGGGG AAGACTGGAA TGAGGTGATG
    TACAATGGCA TTCGCTCCCA GGGTGGGGTC AGCTCAGGCA TGTGGTCTGC CATCTACTTC
    ATTGTGCTCA CCTTGTTTGG AAACTACACG CTGCTAAATG TATTCTTGGC CATCGCTGTG
    GACAATCTGG CCAATGCCCA GGAACTGACC AAGGATGAGC AGGAGGAAGA AGAAGCCTTC
    AACCAGAAAC ATGCACTGCA GAAGGCCAAG GAGGTCAGCC CGATGTCTGC TCCCAACATG
    CCTTCCATTG AAAGAGACAG AAGGAGAAGA CACCACATGT CGATGTGGGA GCCACGCAGC
    AGCCACCTGA GGGAGCGGAG GCGCCGGCAC CACATGTCTG TGTGGGAACA ACGCACCAGC
    CAGCTGAGGA GGCACATGCA AATGTCTAGC CAAGAAGCCC TCAACAAAGA GGAGGCCCCG
    CCAATGAACC CCCTCAATCC ACTCAACCCA CTGAGTCCTC TCAACCCCCT CAATGCTCAC
    CCCAGCCTTT ATCGGAGGCC CAGGCCCATT GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA
    AAGTGTGAAG AAGAACGCAT CAGTCGTGGG GGCTCCCTCA AGGGGGACAT AGGGGGCCTG
    ACTAGCGCCC TGGACAACCA GAGGAGCCCC TTGTCCTTGG GCAAACGGGA GCCACCGTGG
    CTGCCTAGAT CCTGTCATGG AAACTGTGAC CCAATCCAGC AGGAGGCTGG GGGAGGAGAG
    ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG CACAGGCAGA GTCAGAGGCG AAGCCGGCAT
    CGCCGAGTCA GGACTGAGGG CAAGGACTCT GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC
    CAGGAGCGGA GTCTGGATGA AGGGGTGTCC GTTGAGGGGG AGAAGGAACA TGAGCCTCAC
    AGCAGCCACA GGAGCAAAGA GCCGACCATC CACGAAGAGG AGAGAACCCA GGACTTAAGG
    AGGACCAACA GTCTGATGGT CCCCAGGGGC TCTGGACTGG TAGGGGCTCT TGATGAAGCA
    GAAACACCTC TAGTCCAGCC CCAACCTGAG CTGGAAGTGG GGAAGGATGC AGCTCTGACA
    GAGCAGGAGG CTGAAGGCAG CAGTGAACAA GCCCTTCTTG GGGACGTACA GCTGGATGTG
    GGCCGGGGCA TTAGCCAGAG TGAACCGGAT CTCTCCTGCA TGACAGCCAA CATGGACAAG
    GCTACCACGG AGAGCACCAG TGTCACGGTC GCCATCCCTG ATGTGGACCC CTTGGTGGAC
    TCGACAGTGG TGAACATTAG CAACAAGACG GATGGAGAAG CCAGTCCCTT GAAGGAGGCA
    GAGACTAAAG AGGAAGAAGA AGAGGTAGAG AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA
    GAGACTGGAA AAGCCATGGT GCCCCATAGC TCTATGTTCA TCTTCAGCAC CACTAACCCG
    ATCCGCAGGG CCTGCCACTA CATTGTGAAC CTACGCTACT TTGAAATGTG CATTCTCTTG
    GTGATTGCGG CCAGCAGCAT TGCCCTGGCA GCCGAGGACC CTGTACTAAC TAACTCGGAG
    CGTAACAAAG TCCTGAGATA TTTCGACTAT GTTTTCACAG GAGTGTTTAC CTTTGAGATG
    GTTATAAAGA TGATAGACCA AGGCTTGATC CTGCAGGATG GATCATACTT CAGAGACCTC
    TGGAACATCC TGGACTTTGT AGTAGTGGTT GGTGCATTGG TAGCATTTGC TCTGGCGAAC
    GCTTTGGGAA CCAACAAGGG GAGGGATATC AAGACTATCA AGTCTCTGAG GGTACTTCGA
    GTTCTGCGGC CACTGAAAAC CATCAAGCGC TTGCCCAAGC TGAAGGCTGT GTTTGACTGC
    GTGGTGACCT CCTTGAAGAA TGTCTTCAAC ATACTCATTG TCTACAAGCT CTTCATGTTC
    ATCTTTGCCG TCATCGCTGT TCAGCTATTC AAAGGAAAGT TCTTTTATTG CACTGACAGT
    TCCAAGGACA CAGAGAAGGA GTGCATAGGC AACTATGTAG ACCACGAGAA AAACAAGATG
    GAGGTGAAGG GCCGGGAATG GAAGCGTCAT GAATTCCACT ATGACAACAT CATCTGGGCC
    CTGCTTACCC TCTTCACAGT CTCTACAGGG GAAGGATGGC CTCAGGTTCT GCAGCACTCT
    GTAGATGTGA CAGAGGAGGA CAGAGGCCCA AGTCGCAGCA ACCGCATGGA GATGTCCATC
    TTTTATGTGG TCTACTTTGT GGTCTTCCCT TTCTTCTTTG TCAATATCTT CGTGGCTCTC
    ATTATCATCA CCTTCCAGGA GCAAGGGGAC AAGATGATGG AGGAGTGTAG CCTGGAGAAG
    AATGAGCGGG CATGCATCGA CTTCGCCATT AGTGCCAAAC CTCTCACCCG CTACATGCCA
    CAGAACAGGC ACACCTTCCA GTACCGTGTG TGGCACTTTG TAGTCTCCCC ATCCTTTGAG
    TATACCATCA TGGCCATGAT TGCCTTGAAC ACTGTCGTGC TGATGATGAA GTACTACTCT
    GCTCCTTGCA CCTATGAGCT CGCCTTAAAG TACCTGAACA TCGCCTTCAC TATGGTGTTT
    TCCCTGGAGT GTGTCCTAAA GGTCATTGCT TTCGGCTTCC TGAACTATTT CCGAGATACC
    TGGAACATCT TTGACTTCAT CACGGTGATC GGCAGTATCA CAGAAATTAT CCTGACAGAT
    AGCAAGCTGG TGAACACCAG TGGCTTCAAT ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA
    CGGCTCATAA AGCTTCTGAG ACAGGGCTAC ACCATCCGCA TCTTACTGTG GACCTTCGTG
    CAGTCCTTTA AGGCTCTTCC CTACGTCTGC CTTCTCATTG CCATGCTTTT CTTCATCTAC
    GCCATCATTG GGATGCAGGT GTTTGGAAAC ATAAAGTTAG ATGAGGAGAG TCACATCAAC
    CGGCACAACA ACTTCCGGAG TTTCTTTGGA TCCCTCATGC TGCTTTTCAG GAGTGCCACG
    GGGGAGGCTT GGCAGGAGAT TATGCTGTCC TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC
    ACCACTGCAC CCTCAGGGCA GAATGAGAGT GAGCGCTGTG GCACTGACCT GGCCTACGTT
    TATTTTGTCT CCTTCATCTT CTTCTGCTCC TTCCTGATGC TCAACCTGTT CGTGGCTGTC
    ATCATGGACA ACTTTGAGTA CCTGACTCGA GATTCCTCCA TCCTGGGGCC TCACCACTTA
    GATGAGTTTG TCCGTGTCTG GGCAGAATAT GACAGAGCAG CATGTGGCCG CATCCATTAC
    ACTGAGATGT ATGAAATGCT GACTCTCATG TCACCACCGC TAGGCCTCGG CAAGAGATGT
    CCCTCCAAAG TGGCCTATAA GAGGCTGGTC CTGATGAACA TGCCAGTGGC TGAGGACATG
    ACAGTCCACT TCACCTCCAC ACTTATGGCT CTGATCCGGA CAGCTCTGGA CATTAAAATC
    GCCAAAGGTG GTGCAGACAG ACAGCAGCTA GACTCGGAGC TGCAAAAAGA GACCCTGGCC
    ATTTGGCCTC ACCTGTCCCA AAAGATGTTG GATCTGCTTG TGCCCATGCC CAAAGCCTCT
    GACCTGACTG TGGGCAAGAT CTATGCAGCA ATGATGATCA TGGACTACTA TAAACAGAGT
    AAGGTAAAGA AACAGAGACA GCAGCTGGAA GAACAGAAAA ATGCACCCAT GTTCCAGCGC
    ATGGAGCCCT CATCACTGCC TCAGGAGATC ATTGCTAATG CCAAAGCCCT GCCTTATCTC
    CAGCAGGACC CTGTTTCTGG CCTGAGTGGT CGGAGTGGAT ACCCTTCAAT GAGTCCACTC
    TCCCCTCAAG AAATATTCCA GTTGGCTTGT ATGGACCCAG CGGATGATGG ACAATTCCAG
    GAACAACAAT CTCTGGTGAA TGCACAAGAG CCAGAGGTTA GTGAATTAAA AAGTGTGCAG
    TCCTCTAACC ATGGCATCTA CCTTCCTCCG GACACCCAGG AGCATGCGGG ATCTGGGAGG
    GCATCCTCTA TGCCACGTCT GACTATGGAT CCCCAGGTGG TAACAGACCC TAGCTCTATG
    CGACGTTCAT TTTCTACAAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAGTTC
    TCCATGGAAC GCAGCAGTGA AAACACTTAC AAGTCGCGGC GCAGAAGTTA CCATTCCTCC
    CTGAGGCTGT CAGCACACCG CCTGAATTCT GACTCTGGTC ACAAGTCTGA CACTCACCGA
    TCAGGAGGCA GGGAGAGAGG CCGATCCAAA GAACGAAAGC ACCTCCTCTC TCCAGATGTC
    TCCCGCTGCA ATTCTGAAGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAACGTCGC
    CAGTCCAGGT CACCCAGTGA AGGAAGGTCA CAGACCCCCA ATAGACAGGG TACCGGTTCC
    CTAAGTGAGA GCTCCATCCC CTCTATTTCC GACACCAGCA CCCCAAGACG AAGCCGTCGC
    CAGCTCCCAC CTGTGCCACC AAAGCCTCGT CCCCTCCTCT CCTACAGCTC CCTGATGAGA
    CACACTGGCG GCATCTCTCC ACCTCCTGAT GGAAGTGAGG GTGGATCCCC ACTGGCCTCT
    CAAGCTCTGG AGAGCAACAG CGCTTGCCTG ACCGAGTCTT CCAACTCCTT GCACCCCCAG
    CAGGGCCAGC ATCCTTCCCC ACAGCACTAC ATCTCCGAGC CCTATCTGGC CCTCCATGAA
    GACTCCCACG CCTCAGACTG TGGCGAGGAG GAGACACTCA CCTTTGAGGC AGCCGTAGCC
    ACTAGCTTGG GCCGGTCCAA TACCATTGGC TCTGCGCCAC CCCTACGGCA CAGCTGGCAG
    ATGCCTAATG GGCACTATCG GCGGCGGAGG CGTGGGGGGC CTGGGCCTGG CATGATGTGT
    GGAGCTGTCA GTGACCTCCT GAGTGACACG GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168201.1
    CDS594..7904
    Translation

    Target ORF information:

    RefSeq Version XM_017312712.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312712.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGGCAC ACCTCTTGCC
    AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT GGGGCCTCCT ATTTCCGGCA CAAGGAACGG
    CTTCTGCGCA TCTCTATCCG CCACATGGTC AAGTCCCAAG TCTTTTACTG GATCGTCCTG
    AGTGTTGTGG CTCTTAATAC TGCCTGTGTG GCCATTGTTC ATCACAACCA GCCACAGTGG
    CTCACTCACC TCCTCTACTA TGCAGAGTTT TTGTTTCTGG GACTCTTCCT GTTGGAGATG
    TCCCTGAAGA TGTATGGCAT GGGGCCACGC CTCTATTTTC ACTCATCCTT CAACTGCTTT
    GATTTTGGGG TCACAGTGGG CAGCATCTTT GAGGTTGTCT GGGCAATCTT CAGACCTGGC
    ACATCTTTTG GAATCAGTGT CTTACGAGCT CTCCGGCTCC TAAGGATATT TAAAATAACC
    AAGTATTGGG CATCCCTCCG GAATCTGGTT GTCTCCTTGA TGAGCTCCAT GAAGTCTATC
    ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC ATCGTTGTCT TTGCTCTCCT GGGAATGCAG
    CTCTTTGGAG GCAGGTTTAA CTTTAATGAT GGGACTCCTT CAGCTAATTT TGACACCTTC
    CCTGCAGCTA TCATGACAGT GTTCCAGATC CTGACAGGGG AAGACTGGAA TGAGGTGATG
    TACAATGGCA TTCGCTCCCA GGGTGGGGTC AGCTCAGGCA TGTGGTCTGC CATCTACTTC
    ATTGTGCTCA CCTTGTTTGG AAACTACACG CTGCTAAATG TATTCTTGGC CATCGCTGTG
    GACAATCTGG CCAATGCCCA GGAACTGACC AAGGATGAGC AGGAGGAAGA AGAAGCCTTC
    AACCAGAAAC ATGCACTGCA GAAGGCCAAG GAGGTCAGCC CGATGTCTGC TCCCAACATG
    CCTTCCATTG AAAGAGACAG AAGGAGAAGA CACCACATGT CGATGTGGGA GCCACGCAGC
    AGCCACCTGA GGGAGCGGAG GCGCCGGCAC CACATGTCTG TGTGGGAACA ACGCACCAGC
    CAGCTGAGGA GGCACATGCA AATGTCTAGC CAAGAAGCCC TCAACAAAGA GGAGGCCCCG
    CCAATGAACC CCCTCAATCC ACTCAACCCA CTGAGTCCTC TCAACCCCCT CAATGCTCAC
    CCCAGCCTTT ATCGGAGGCC CAGGCCCATT GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA
    AAGTGTGAAG AAGAACGCAT CAGTCGTGGG GGCTCCCTCA AGGGGGACAT AGGGGGCCTG
    ACTAGCGCCC TGGACAACCA GAGGAGCCCC TTGTCCTTGG GCAAACGGGA GCCACCGTGG
    CTGCCTAGAT CCTGTCATGG AAACTGTGAC CCAATCCAGC AGGAGGCTGG GGGAGGAGAG
    ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG CACAGGCAGA GTCAGAGGCG AAGCCGGCAT
    CGCCGAGTCA GGACTGAGGG CAAGGACTCT GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC
    CAGGAGCGGA GTCTGGATGA AGGGGTGTCC GTTGAGGGGG AGAAGGAACA TGAGCCTCAC
    AGCAGCCACA GGAGCAAAGA GCCGACCATC CACGAAGAGG AGAGAACCCA GGACTTAAGG
    AGGACCAACA GTCTGATGGT CCCCAGGGGC TCTGGACTGG TAGGGGCTCT TGATGAAGCA
    GAAACACCTC TAGTCCAGCC CCAACCTGAG CTGGAAGTGG GGAAGGATGC AGCTCTGACA
    GAGCAGGAGG CTGAAGGCAG CAGTGAACAA GCCCTTCTTG GGGACGTACA GCTGGATGTG
    GGCCGGGGCA TTAGCCAGAG TGAACCGGAT CTCTCCTGCA TGACAGCCAA CATGGACAAG
    GCTACCACGG AGAGCACCAG TGTCACGGTC GCCATCCCTG ATGTGGACCC CTTGGTGGAC
    TCGACAGTGG TGAACATTAG CAACAAGACG GATGGAGAAG CCAGTCCCTT GAAGGAGGCA
    GAGACTAAAG AGGAAGAAGA AGAGGTAGAG AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA
    GAGACTGGAA AAGCCATGGT GCCCCATAGC TCTATGTTCA TCTTCAGCAC CACTAACCCG
    ATCCGCAGGG CCTGCCACTA CATTGTGAAC CTACGCTACT TTGAAATGTG CATTCTCTTG
    GTGATTGCGG CCAGCAGCAT TGCCCTGGCA GCCGAGGACC CTGTACTAAC TAACTCGGAG
    CGTAACAAAG TCCTGAGATA TTTCGACTAT GTTTTCACAG GAGTGTTTAC CTTTGAGATG
    GTTATAAAGA TGATAGACCA AGGCTTGATC CTGCAGGATG GATCATACTT CAGAGACCTC
    TGGAACATCC TGGACTTTGT AGTAGTGGTT GGTGCATTGG TAGCATTTGC TCTGGCGAAC
    GCTTTGGGAA CCAACAAGGG GAGGGATATC AAGACTATCA AGTCTCTGAG GGTACTTCGA
    GTTCTGCGGC CACTGAAAAC CATCAAGCGC TTGCCCAAGC TGAAGGCTGT GTTTGACTGC
    GTGGTGACCT CCTTGAAGAA TGTCTTCAAC ATACTCATTG TCTACAAGCT CTTCATGTTC
    ATCTTTGCCG TCATCGCTGT TCAGCTATTC AAAGGAAAGT TCTTTTATTG CACTGACAGT
    TCCAAGGACA CAGAGAAGGA GTGCATAGGC AACTATGTAG ACCACGAGAA AAACAAGATG
    GAGGTGAAGG GCCGGGAATG GAAGCGTCAT GAATTCCACT ATGACAACAT CATCTGGGCC
    CTGCTTACCC TCTTCACAGT CTCTACAGGG GAAGGATGGC CTCAGGTTCT GCAGCACTCT
    GTAGATGTGA CAGAGGAGGA CAGAGGCCCA AGTCGCAGCA ACCGCATGGA GATGTCCATC
    TTTTATGTGG TCTACTTTGT GGTCTTCCCT TTCTTCTTTG TCAATATCTT CGTGGCTCTC
    ATTATCATCA CCTTCCAGGA GCAAGGGGAC AAGATGATGG AGGAGTGTAG CCTGGAGAAG
    AATGAGCGGG CATGCATCGA CTTCGCCATT AGTGCCAAAC CTCTCACCCG CTACATGCCA
    CAGAACAGGC ACACCTTCCA GTACCGTGTG TGGCACTTTG TAGTCTCCCC ATCCTTTGAG
    TATACCATCA TGGCCATGAT TGCCTTGAAC ACTGTCGTGC TGATGATGAA GTACTACTCT
    GCTCCTTGCA CCTATGAGCT CGCCTTAAAG TACCTGAACA TCGCCTTCAC TATGGTGTTT
    TCCCTGGAGT GTGTCCTAAA GGTCATTGCT TTCGGCTTCC TGAACTATTT CCGAGATACC
    TGGAACATCT TTGACTTCAT CACGGTGATC GGCAGTATCA CAGAAATTAT CCTGACAGAT
    AGCAAGCTGG TGAACACCAG TGGCTTCAAT ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA
    CGGCTCATAA AGCTTCTGAG ACAGGGCTAC ACCATCCGCA TCTTACTGTG GACCTTCGTG
    CAGTCCTTTA AGGCTCTTCC CTACGTCTGC CTTCTCATTG CCATGCTTTT CTTCATCTAC
    GCCATCATTG GGATGCAGGT GTTTGGAAAC ATAAAGTTAG ATGAGGAGAG TCACATCAAC
    CGGCACAACA ACTTCCGGAG TTTCTTTGGA TCCCTCATGC TGCTTTTCAG GAGTGCCACG
    GGGGAGGCTT GGCAGGAGAT TATGCTGTCC TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC
    ACCACTGCAC CCTCAGGGCA GAATGAGAGT GAGCGCTGTG GCACTGACCT GGCCTACGTT
    TATTTTGTCT CCTTCATCTT CTTCTGCTCC TTCCTGATGC TCAACCTGTT CGTGGCTGTC
    ATCATGGACA ACTTTGAGTA CCTGACTCGA GATTCCTCCA TCCTGGGGCC TCACCACTTA
    GATGAGTTTG TCCGTGTCTG GGCAGAATAT GACAGAGCAG CATGTGGCCG CATCCATTAC
    ACTGAGATGT ATGAAATGCT GACTCTCATG TCACCACCGC TAGGCCTCGG CAAGAGATGT
    CCCTCCAAAG TGGCCTATAA GAGGCTGGTC CTGATGAACA TGCCAGTGGC TGAGGACATG
    ACAGTCCACT TCACCTCCAC ACTTATGGCT CTGATCCGGA CAGCTCTGGA CATTAAAATC
    GCCAAAGGTG GTGCAGACAG ACAGCAGCTA GACTCGGAGC TGCAAAAAGA GACCCTGGCC
    ATTTGGCCTC ACCTGTCCCA AAAGATGTTG GATCTGCTTG TGCCCATGCC CAAAGCCTCT
    GACCTGACTG TGGGCAAGAT CTATGCAGCA ATGATGATCA TGGACTACTA TAAACAGAGT
    AAGGTAAAGA AACAGAGACA GCAGCTGGAA GAACAGAAAA ATGCACCCAT GTTCCAGCGC
    ATGGAGCCCT CATCACTGCC TCAGGAGATC ATTGCTAATG CCAAAGCCCT GCCTTATCTC
    CAGCAGGACC CTGTTTCTGG CCTGAGTGGT CGGAGTGGAT ACCCTTCAAT GAGTCCACTC
    TCCCCTCAAG AAATATTCCA GTTGGCTTGT ATGGACCCAG CGGATGATGG ACAATTCCAG
    GAACAACAAT CTCTGGTGAA TGCACAAGAG CCAGAGGTTA GTGAATTAAA AAGTGTGCAG
    TCCTCTAACC ATGGCATCTA CCTTCCTCCG GACACCCAGG AGCATGCGGG ATCTGGGAGG
    GCATCCTCTA TGCCACGTCT GACTATGGAT CCCCAGGTGG TAACAGACCC TAGCTCTATG
    CGACGTTCAT TTTCTACAAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAGTTC
    TCCATGGAAC GCAGCAGTGA AAACACTTAC AAGTCGCGGC GCAGAAGTTA CCATTCCTCC
    CTGAGGCTGT CAGCACACCG CCTGAATTCT GACTCTGGTC ACAAGTCTGA CACTCACCGA
    TCAGGAGGCA GGGAGAGAGG CCGATCCAAA GAACGAAAGC ACCTCCTCTC TCCAGATGTC
    TCCCGCTGCA ATTCTGAAGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAACGTCGC
    CAGTCCAGGT CACCCAGTGA AGGAAGGTCA CAGACCCCCA ATAGACAGGG TACCGGTTCC
    CTAAGTGAGA GCTCCATCCC CTCTATTTCC GACACCAGCA CCCCAAGACG AAGCCGTCGC
    CAGCTCCCAC CTGTGCCACC AAAGCCTCGT CCCCTCCTCT CCTACAGCTC CCTGATGAGA
    CACACTGGCG GCATCTCTCC ACCTCCTGAT GGAAGTGAGG GTGGATCCCC ACTGGCCTCT
    CAAGCTCTGG AGAGCAACAG CGCTTGCCTG ACCGAGTCTT CCAACTCCTT GCACCCCCAG
    CAGGGCCAGC ATCCTTCCCC ACAGCACTAC ATCTCCGAGC CCTATCTGGC CCTCCATGAA
    GACTCCCACG CCTCAGACTG TGGCGAGGAG GAGACACTCA CCTTTGAGGC AGCCGTAGCC
    ACTAGCTTGG GCCGGTCCAA TACCATTGGC TCTGCGCCAC CCCTACGGCA CAGCTGGCAG
    ATGCCTAATG GGCACTATCG GCGGCGGAGG CGTGGGGGGC CTGGGCCTGG CATGATGTGT
    GGAGCTGTCA GTGACCTCCT GAGTGACACG GAAGAAGACG ATAAGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67266
    Clone ID Related Accession (Same CDS sequence) XM_017312730.1
    Accession Version XM_017312730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGTG
    GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT
    TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG
    CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT
    CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG
    CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC
    TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC
    AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC
    ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC
    TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG
    GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT
    TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG
    CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC
    ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA
    CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG
    CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC
    GATAAGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168219.1
    CDS594..7865
    Translation

    Target ORF information:

    RefSeq Version XM_017312730.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC
    GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG
    ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT
    TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG
    GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC
    ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG
    CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC
    AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG
    GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC
    ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG
    CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC
    CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG
    GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC
    TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC
    CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT
    GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC
    CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA
    GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT
    CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC
    GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG
    AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC
    CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC
    AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC
    TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC
    AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG
    TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT
    TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC
    CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA
    TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC
    TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC
    CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC
    ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGTG
    GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT
    TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG
    CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT
    CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG
    CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC
    TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC
    AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC
    ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC
    TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG
    GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT
    TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG
    CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC
    ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA
    CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG
    CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC
    GATAAGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67267
    Clone ID Related Accession (Same CDS sequence) XM_011247916.3 , XM_011247916.2 , XM_017312848.1
    Accession Version XM_011247916.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA
    AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG
    GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246218.1
    CDS11493..18455
    Translation

    Target ORF information:

    RefSeq Version XM_011247916.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247916.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA
    AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG
    GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu67267
    Clone ID Related Accession (Same CDS sequence) XM_011247916.3 , XM_011247916.2 , XM_017312848.1
    Accession Version XM_017312848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG
    AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT
    GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT
    TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG
    AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC
    ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT
    CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC
    TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT
    AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC
    CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC
    CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC
    ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT
    TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT
    ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC
    TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT
    TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC
    AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT
    TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC
    CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG
    CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG
    GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA
    GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC
    ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA
    ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT
    GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA
    AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG
    GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC
    CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG
    TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT
    TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT
    GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC
    TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC
    CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG
    GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA
    CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC
    TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC
    CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC
    TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG
    GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG
    GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG
    CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT
    GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168337.1
    CDS581..7543
    Translation

    Target ORF information:

    RefSeq Version XM_017312848.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT
    GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC
    TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG
    CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA
    TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC
    TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT
    TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG
    AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG
    GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA
    CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC
    CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT
    CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC
    ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG
    GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA
    CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT
    CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC
    AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC
    CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG
    AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC
    ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG
    TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA
    ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC
    TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT
    GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CA