Crygs cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Crygs
Entrez Gene ID	12970
Full Name	crystallin, gamma S
Synonyms	AI327013,Opj,rncat
General protein information	Preferred Names crystallin, gamma S Names beta-crystallin S opacity due to poor secondary fiber cell junction recessive nuclear cataract
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 B1\|16 13.66 cM

mRNA and Protein(s)

mRNA Protein Name

XM_006521755.2 XP_006521818.1 gamma-crystallin S isoform X1

NM_009967.2 NP_034097.1 gamma-crystallin S
Pathways from BioSystems

mRNA	Protein	Name
XM_006521755.2	XP_006521818.1	gamma-crystallin S isoform X1
NM_009967.2	NP_034097.1	gamma-crystallin S

Homologies

Mus musculus (house mouse)	Crygs	NP_034097.1
Rattus norvegicus (Norway rat)	Crygs	NP_001103023.1
Danio rerio (zebrafish)	crygs1	NP_001013294.1
Bos taurus (cattle)	CRYGS	NP_776717.1
Gallus gallus (chicken)	CRYGS	NP_001163998.1
Pan troglodytes (chimpanzee)	CRYGS	XP_516929.1
	CRYGS	XP_001092090.1
Danio rerio (zebrafish)	crygs2	NP_001012262.1
Homo sapiens (human)	CRYGS	NP_060011.1
Canis lupus familiaris (dog)	CRYGS	NP_001074370.1

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of Rest Leads to the Early Onset of Cataracts with the Aberrant Terminal Differentiation of Lens Fiber Cells.
Aoki H, Ogino H, Tomita H, Hara A, Kunisada T
PloS one11(9)e0163042(2016)

A role for ?S-crystallin in the organization of actin and fiber cell maturation in the mouse lens.
Fan J, Dong L, Mishra S, Chen Y, FitzGerald P, Wistow G
The FEBS journal279(16)2892-904(2012 Aug)

The transcription factor c-Maf controls touch receptor development and function.
Wende H, Lechner SG, Cheret C, Bourane S, Kolanczyk ME, Pattyn A, Reuter K, Munier FL, Carroll P, Lewin GR, Birchmeier C
Science (New York, N.Y.)335(6074)1373-6(2012 Mar)

Alterations to proteins in the lens of hereditary Crygs-mutated cataractous mice.
Ji Y, Bi H, Li N, Jin H, Yang P, Kong X, Yan S, Lu Y
Molecular vision161068-75(2010 Jun)

Removal of Hsf4 leads to cataract development in mice through down-regulation of gamma S-crystallin and Bfsp expression.
Shi X, Cui B, Wang Z, Weng L, Xu Z, Ma J, Xu G, Kong X, Hu L
BMC molecular biology1010(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gammaS may have a functional role related to actin, perhaps in 'shepherding' filaments to maintain the optical properties of the lens cytoplasm and normal fiber cell maturation
Title: A role for ?S-crystallin in the organization of actin and fiber cell maturation in the mouse lens.

Mutant Crygs gene can lead to changes of BFSP/filensin and other crystallins. Changes to these crystallins, together, may secondarily lead to cataract formation.
Title: Alterations to proteins in the lens of hereditary Crygs-mutated cataractous mice.

disruption of the Hsf4 gene leads to cataracts via at least three pathways: down-regulation of gamma-crystallin, particularly gamma S-crystallin; decreased lens beaded filament expression; and loss of post-translational modification of alpha A-crystallin
Title: Removal of Hsf4 leads to cataract development in mice through down-regulation of gamma S-crystallin and Bfsp expression.

gammaS consists of two topologically similar domains, arranged with an approximate twofold symmetry, and each domain shows close structural homology to closely related domains found in other members of the gamma-crystallin family.
Title: Solution structure of (gamma)S-crystallin by molecular fragment replacement NMR.

mutation causes autosomal recessive cataracts; likely to be involved in epithelial cell proliferation, apoptosis, and migration
Title: The gamma S-crystallin gene is mutated in autosomal recessive cataract in mouse.

The following Crygs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crygs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu51844	XM_006521755.2 Latest version!	Mus musculus crystallin, gamma S (Crygs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu05233	NM_009967.2 Latest version!	Mus musculus crystallin, gamma S (Crygs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crygs ( XM_006521755.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521818.1 Mus musculus crystallin, gamma S (Crygs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51844
Clone ID Related Accession (Same CDS sequence)	XM_006521755.2
Accession Version	XM_006521755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	gamma-crystallin S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCT TACCCCAGGG CGAGTACCCT GAATACCAGC GTTGGATGGG CCTTAATGAC 
CGCCTGGGCT CTTGCCGAGC TGTTCATCTG TCTAGCGGAG GCCAGGCTAA GATTCAGGTC 
TTTGAAAAGG GCGACTTCAA CGGTCAGATG TACGAAACCA CGGAAGACTG TCCTTCTATC 
ATGGAGCAGT TTCACCTGCG AGAGATTCAT TCCTGTAAGG TGGTAGAAGG CACCTGGATT 
TTCTATGAGC TACCCAACTA CCGTGGCAGA CAGTATCTTC TGGATAAGAA GGAGTACCGG 
AAGCCCGTCG ATTGGGGTGC GGCATCCCCC GCTATCCAGT CGTTCCGCCG CATTGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521818.1
CDS	400..762
Translation	MYILPQGEYPEYQRWMGLNDRLGSCRAVHLSSGGQAKIQVFEKGDFNGQMYETTEDCPSIMEQFHLREIHSCKVVEGTWIFYELPNYRGRQYLLDKKEYRKPVDWGAASPAIQSFRRIVE

Target ORF information:

RefSeq Version	XM_006521755.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, gamma S (Crygs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521755.2

ORF Insert Sequence:

ATGTACATCT TACCCCAGGG CGAGTACCCT GAATACCAGC GTTGGATGGG CCTTAATGAC 
CGCCTGGGCT CTTGCCGAGC TGTTCATCTG TCTAGCGGAG GCCAGGCTAA GATTCAGGTC 
TTTGAAAAGG GCGACTTCAA CGGTCAGATG TACGAAACCA CGGAAGACTG TCCTTCTATC 
ATGGAGCAGT TTCACCTGCG AGAGATTCAT TCCTGTAAGG TGGTAGAAGG CACCTGGATT 
TTCTATGAGC TACCCAACTA CCGTGGCAGA CAGTATCTTC TGGATAAGAA GGAGTACCGG 
AAGCCCGTCG ATTGGGGTGC GGCATCCCCC GCTATCCAGT CGTTCCGCCG CATTGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crygs ( NM_009967.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034097.1 Mus musculus crystallin, gamma S (Crygs), mRNA.

Price & Availability↑

CloneID	OMu05233
Clone ID Related Accession (Same CDS sequence)	NM_009967.2
Accession Version	NM_009967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	gamma-crystallin S
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT571248.10 and AK143060.1. On Mar 18, 2010 this sequence version replaced NM_009967.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB845195.2, AF032995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTAAAA CTGGAGGCAA GATTAGTTTC TACGAAGACC GAAATTTTCA AGGCCGCCGC 
TATGACTGCG ACTGCGACTG CGCAGACTTC CGCTCGTACC TAAGTCGCTG CAACTCCATT 
AGAGTAGAGG GAGGCACCTG GGCTGTGTAT GAAAGGCCCA ACTTCTCCGG GCACATGTAC 
ATCTTACCCC AGGGCGAGTA CCCTGAATAC CAGCGTTGGA TGGGCCTTAA TGACCGCCTG 
GGCTCTTGCC GAGCTGTTCA TCTGTCTAGC GGAGGCCAGG CTAAGATTCA GGTCTTTGAA 
AAGGGCGACT TCAACGGTCA GATGTACGAA ACCACGGAAG ACTGTCCTTC TATCATGGAG 
CAGTTTCACC TGCGAGAGAT TCATTCCTGT AAGGTGGTAG AAGGCACCTG GATTTTCTAT 
GAGCTACCCA ACTACCGTGG CAGACAGTAT CTTCTGGATA AGAAGGAGTA CCGGAAGCCC 
GTCGATTGGG GTGCGGCATC CCCCGCTATC CAGTCGTTCC GCCGCATTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034097.1
CDS	56..592
Translation	MSKTGGKISFYEDRNFQGRRYDCDCDCADFRSYLSRCNSIRVEGGTWAVYERPNFSGHMYILPQGEYPEYQRWMGLNDRLGSCRAVHLSSGGQAKIQVFEKGDFNGQMYETTEDCPSIMEQFHLREIHSCKVVEGTWIFYELPNYRGRQYLLDKKEYRKPVDWGAASPAIQSFRRIVE

Target ORF information:

RefSeq Version	NM_009967.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, gamma S (Crygs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009967.2

ORF Insert Sequence:

ATGTCTAAAA CTGGAGGCAA GATTAGTTTC TACGAAGACC GAAATTTTCA AGGCCGCCGC 
TATGACTGCG ACTGCGACTG CGCAGACTTC CGCTCGTACC TAAGTCGCTG CAACTCCATT 
AGAGTAGAGG GAGGCACCTG GGCTGTGTAT GAAAGGCCCA ACTTCTCCGG GCACATGTAC 
ATCTTACCCC AGGGCGAGTA CCCTGAATAC CAGCGTTGGA TGGGCCTTAA TGACCGCCTG 
GGCTCTTGCC GAGCTGTTCA TCTGTCTAGC GGAGGCCAGG CTAAGATTCA GGTCTTTGAA 
AAGGGCGACT TCAACGGTCA GATGTACGAA ACCACGGAAG ACTGTCCTTC TATCATGGAG 
CAGTTTCACC TGCGAGAGAT TCATTCCTGT AAGGTGGTAG AAGGCACCTG GATTTTCTAT 
GAGCTACCCA ACTACCGTGG CAGACAGTAT CTTCTGGATA AGAAGGAGTA CCGGAAGCCC 
GTCGATTGGG GTGCGGCATC CCCCGCTATC CAGTCGTTCC GCCGCATTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of Rest Leads to the Early Onset of Cataracts with the Aberrant Terminal Differentiation of Lens Fiber Cells.
PloS one11(9)e0163042(2016)
Aoki H,Ogino H,Tomita H,Hara A,Kunisada T

A role for ?S-crystallin in the organization of actin and fiber cell maturation in the mouse lens.
The FEBS journal279(16)2892-904(2012 Aug)
Fan J,Dong L,Mishra S,Chen Y,FitzGerald P,Wistow G

The transcription factor c-Maf controls touch receptor development and function.
Science (New York, N.Y.)335(6074)1373-6(2012 Mar)
Wende H,Lechner SG,Cheret C,Bourane S,Kolanczyk ME,Pattyn A,Reuter K,Munier FL,Carroll P,Lewin GR,Birchmeier C

Alterations to proteins in the lens of hereditary Crygs-mutated cataractous mice.
Molecular vision161068-75(2010 Jun)
Ji Y,Bi H,Li N,Jin H,Yang P,Kong X,Yan S,Lu Y

Removal of Hsf4 leads to cataract development in mice through down-regulation of gamma S-crystallin and Bfsp expression.
BMC molecular biology1010(2009 Feb)
Shi X,Cui B,Wang Z,Weng L,Xu Z,Ma J,Xu G,Kong X,Hu L

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Crygs cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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