Gja4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Gja4
Entrez Gene ID	14612
Full Name	gap junction protein, alpha 4
Synonyms	AU020209,AW558810,Cnx37,Cx37,Gja-4
General protein information	Preferred Names gap junction protein, alpha 4 Names gap junction alpha-4 protein connexin-37 gap junction membrane channel protein alpha 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D2.2\|4 61.47 cM

mRNA and Protein(s)

mRNA Protein Name

NM_008120.3 NP_032146.1 gap junction alpha-4 protein
Pathways from BioSystems

mRNA	Protein	Name
NM_008120.3	NP_032146.1	gap junction alpha-4 protein

Homologies

Homo sapiens (human)	GJA4	NP_002051.2
Bos taurus (cattle)	GJA4	NP_001077207.1
Pan troglodytes (chimpanzee)	GJA4	XP_001166110.1
	LOC710913	XP_001108418.1
Canis lupus familiaris (dog)	GJA4	XP_005629029.1
Xenopus tropicalis (tropical clawed frog)	gja4	NP_001072904.1
Mus musculus (house mouse)	Gja4	NP_032146.1
Rattus norvegicus (Norway rat)	Gja4	NP_067686.1
Gallus gallus (chicken)	GJA4	NP_996867.3

Gene Ontology
Bibliography

Related articles in PubMed

NO Augments Endothelial Reactivity by Reducing Myoendothelial Calcium Signal Spreading: A Novel Role for Cx37 (Connexin 37) and the Protein Tyrosine Phosphatase SHP-2.
Pogoda K, Mannell H, Blodow S, Schneider H, Schubert KM, Qiu J, Schmidt A, Imhof A, Beck H, Tanase LI, Pfeifer A, Pohl U, Kameritsch P
Arteriosclerosis, thrombosis, and vascular biology37(12)2280-2290(2017 Dec)

Hdac3 regulates lymphovenous and lymphatic valve formation.
Janardhan HP, Milstone ZJ, Shin M, Lawson ND, Keaney JF Jr, Trivedi CM
The Journal of clinical investigation127(11)4193-4206(2017 Nov)

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Wang Y, Wu B, Lu P, Zhang D, Wu B, Varshney S, Del Monte-Nieto G, Zhuang Z, Charafeddine R, Kramer AH, Sibinga NE, Frangogiannis NG, Kitsis RN, Adams RH, Alitalo K, Sharp DJ, Harvey RP, Stanley P, Zhou B
Nature communications8(1)578(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NO controls the calcium signal propagation through Cx37-containing gap junctions. The tyrosine phosphatase SHP-2 is the essential mediator and NO target.
Title: NO Augments Endothelial Reactivity by Reducing Myoendothelial Calcium Signal Spreading: A Novel Role for Cx37 (Connexin 37) and the Protein Tyrosine Phosphatase SHP-2.

altered profile of the bone matrix components in Cx37-deficient mice acts in favor of higher resistance to fracture in long bones.
Title: Connexin37 deficiency alters organic bone matrix, cortical bone geometry, and increases Wnt/?-catenin signaling.

Data show that segregation of Foxc2 and NFATc1 transcription factor is closely associated with the highly polarized expression of connexins Cx37, Cx43, and Cx47.
Title: Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice.

Cx37 deletion increased the size of atherosclerotic lesions in oscillatory shear stress regions and abrogated the development of a stable plaque phenotype under OSS in ApoE(-/-) mice
Title: Shear stress-induced atherosclerotic plaque composition in ApoE(-/-) mice is modulated by connexin37.

administration of 0.3 IU/mL FSH during ovarian cryopreservation by vitrification can maintain ovarian survival during ovarian vitrification and increases the blood supply with avascular transplantation via upregulation of Cx43, Cx37, and VEGF/VEGFR2
Title: The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone.

The following Gja4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gja4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01382	NM_008120.3 Latest version!	Mus musculus gap junction protein, alpha 4 (Gja4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gja4 ( NM_008120.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032146.1 Mus musculus gap junction protein, alpha 4 (Gja4), mRNA.

Price & Availability↑

CloneID	OMu01382
Clone ID Related Accession (Same CDS sequence)	NM_008120.3
Accession Version	NM_008120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	gap junction alpha-4 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143024.1 and AL626768.21. On Oct 27, 2012 this sequence version replaced NM_008120.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143024.1, AF216832.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACT GGGGCTTCCT GGAGAAGTTG CTAGACCAGG TCCAGGAACA CTCGACCGTG 
GTGGGCAAGA TCTGGTTAAC GGTGCTCTTC ATCTTCCGCA TCCTCATCCT GGGGCTGGCT 
GGCGAGTCGG TGTGGGGCGA CGAGCAGTCT GATTTTGAGT GTAACACAGC CCAGCCGGGC 
TGCACCAACG TCTGCTATGA CCAGGCCTTC CCCATCTCCC ACATCCGATA CTGGGTGCTG 
CAGTTCCTCT TCGTCAGCAC ACCCACCCTG ATCTACCTGG GCCACGTCAT CTACCTGTCT 
CGGCGGGAAG AGCGGTTGCG GCAGAAAGAG GGAGAGCTCC GGGCGCTGCC ATCCAAGGAC 
CTACATGTAG AGCGGGCACT GGCTGCCATC GAACATCAGA TGGCCAAGAT CTCGGTGGCA 
GAGGACGGTC GTCTTCGGAT TCGTGGGGCG CTCATGGGTA CCTATGTGGT CAGCGTGCTG 
TGTAAGAGTG TGCTGGAGGC AGGCTTCCTC TATGGCCAGT GGCGCCTCTA TGGCTGGACC 
ATGGAGCCGG TGTTTGTGTG CCAGCGTGCG CCCTGCCCCC ACATCGTGGA CTGCTATGTC 
TCTCGACCCA CTGAGAAGAC TATCTTCATC ATCTTCATGC TGGTGGTAGG AGTCATCTCC 
CTGGTGCTCA ACCTGCTGGA GCTGGTTCAC CTGCTGTGTC GGTGTGTCAG CCGGGAGATA 
AAGGCACGAA GGGACCACGA CGCCCGCCCG GCCCAGGGCA GTGCCTCAGA CCCTTACCCT 
GAACAGGTTT TCTTCTACCT CCCCATGGGC GAGGGACCCT CTTCCCCACC GTGTCCCACC 
TACAACGGGC TCTCATCCAC TGAGCAGAAC TGGGCCAACT TGACCACAGA GGAGAGACTG 
ACCTCTTCCA GACCTCCCCC ATTTGTAAAC ACAGCTCCCC AGGGTGGCCG AAAGTCCCCT 
AGCCGCCCCA ACAGCTCTGC ATCCAAGAAG CAGTATGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032146.1
CDS	138..1139
Translation	MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPGCTNVCYDQAFPISHIRYWVLQFLFVSTPTLIYLGHVIYLSRREERLRQKEGELRALPSKDLHVERALAAIEHQMAKISVAEDGRLRIRGALMGTYVVSVLCKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPHIVDCYVSRPTEKTIFIIFMLVVGVISLVLNLLELVHLLCRCVSREIKARRDHDARPAQGSASDPYPEQVFFYLPMGEGPSSPPCPTYNGLSSTEQNWANLTTEERLTSSRPPPFVNTAPQGGRKSPSRPNSSASKKQYV

Target ORF information:

RefSeq Version	NM_008120.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, alpha 4 (Gja4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008120.3

ORF Insert Sequence:

ATGGGCGACT GGGGCTTCCT GGAGAAGTTG CTAGACCAGG TCCAGGAACA CTCGACCGTG 
GTGGGCAAGA TCTGGTTAAC GGTGCTCTTC ATCTTCCGCA TCCTCATCCT GGGGCTGGCT 
GGCGAGTCGG TGTGGGGCGA CGAGCAGTCT GATTTTGAGT GTAACACAGC CCAGCCGGGC 
TGCACCAACG TCTGCTATGA CCAGGCCTTC CCCATCTCCC ACATCCGATA CTGGGTGCTG 
CAGTTCCTCT TCGTCAGCAC ACCCACCCTG ATCTACCTGG GCCACGTCAT CTACCTGTCT 
CGGCGGGAAG AGCGGTTGCG GCAGAAAGAG GGAGAGCTCC GGGCGCTGCC ATCCAAGGAC 
CTACATGTAG AGCGGGCACT GGCTGCCATC GAACATCAGA TGGCCAAGAT CTCGGTGGCA 
GAGGACGGTC GTCTTCGGAT TCGTGGGGCG CTCATGGGTA CCTATGTGGT CAGCGTGCTG 
TGTAAGAGTG TGCTGGAGGC AGGCTTCCTC TATGGCCAGT GGCGCCTCTA TGGCTGGACC 
ATGGAGCCGG TGTTTGTGTG CCAGCGTGCG CCCTGCCCCC ACATCGTGGA CTGCTATGTC 
TCTCGACCCA CTGAGAAGAC TATCTTCATC ATCTTCATGC TGGTGGTAGG AGTCATCTCC 
CTGGTGCTCA ACCTGCTGGA GCTGGTTCAC CTGCTGTGTC GGTGTGTCAG CCGGGAGATA 
AAGGCACGAA GGGACCACGA CGCCCGCCCG GCCCAGGGCA GTGCCTCAGA CCCTTACCCT 
GAACAGGTTT TCTTCTACCT CCCCATGGGC GAGGGACCCT CTTCCCCACC GTGTCCCACC 
TACAACGGGC TCTCATCCAC TGAGCAGAAC TGGGCCAACT TGACCACAGA GGAGAGACTG 
ACCTCTTCCA GACCTCCCCC ATTTGTAAAC ACAGCTCCCC AGGGTGGCCG AAAGTCCCCT 
AGCCGCCCCA ACAGCTCTGC ATCCAAGAAG CAGTATGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NO Augments Endothelial Reactivity by Reducing Myoendothelial Calcium Signal Spreading: A Novel Role for Cx37 (Connexin 37) and the Protein Tyrosine Phosphatase SHP-2.
Arteriosclerosis, thrombosis, and vascular biology37(12)2280-2290(2017 Dec)
Pogoda K,Mannell H,Blodow S,Schneider H,Schubert KM,Qiu J,Schmidt A,Imhof A,Beck H,Tanase LI,Pfeifer A,Pohl U,Kameritsch P

Hdac3 regulates lymphovenous and lymphatic valve formation.
The Journal of clinical investigation127(11)4193-4206(2017 Nov)
Janardhan HP,Milstone ZJ,Shin M,Lawson ND,Keaney JF Jr,Trivedi CM

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Nature communications8(1)578(2017 09)
Wang Y,Wu B,Lu P,Zhang D,Wu B,Varshney S,Del Monte-Nieto G,Zhuang Z,Charafeddine R,Kramer AH,Sibinga NE,Frangogiannis NG,Kitsis RN,Adams RH,Alitalo K,Sharp DJ,Harvey RP,Stanley P,Zhou B

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Gja4 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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