Eif2ak1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Eif2ak1
Entrez Gene ID	15467
Full Name	eukaryotic translation initiation factor 2 alpha kinase 1
Synonyms	HCR,Hri
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 1 Names eukaryotic translation initiation factor 2-alpha kinase 1 heme-controlled repressor heme-regulated eukaryotic initiation factor eIF-2-alpha kinase heme-regulated inhibitor hemin-sensitive initiation factor 2-alpha kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320685.1	XP_017176174.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
XM_017320685.2	XP_017176174.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
NM_013557.2	NP_038585.2	eukaryotic translation initiation factor 2-alpha kinase 1

Pathways from BioSystems

Homologies

	EIF2AK1	XP_001089983.2
Canis lupus familiaris (dog)	EIF2AK1	NP_001155185.1
Rattus norvegicus (Norway rat)	Eif2ak1	NP_037355.1
Danio rerio (zebrafish)	eif2ak1	NP_001071035.1
Schizosaccharomyces pombe (fission yeast)	hri2	NP_593146.1
Gallus gallus (chicken)	EIF2AK1	NP_989979.1
Pan troglodytes (chimpanzee)	EIF2AK1	XP_518962.3
Homo sapiens (human)	EIF2AK1	NP_001127807.1
Mus musculus (house mouse)	Eif2ak1	NP_038585.2
Xenopus tropicalis (tropical clawed frog)	eif2ak1	NP_001039038.1
Bos taurus (cattle)	EIF2AK1	NP_001033142.1
Schizosaccharomyces pombe (fission yeast)	hri1	NP_594738.1

Gene Ontology
Bibliography

Related articles in PubMed

The eIF2? kinase Heme Regulated Inhibitor (HRI) protects the host from infection by regulating intracellular pathogen trafficking.
Bahnan W, Boucher JC, Gayle P, Shrestha N, Rosen M, Aktas B, Adkins B, Ager A, Khan WN, Schesser K
Infection and immunity(2018 Jan)

HRI coordinates translation by eIF2?P and mTORC1 to mitigate ineffective erythropoiesis in mice during iron deficiency.
Zhang S, Macias-Garcia A, Velazquez J, Paltrinieri E, Kaufman RJ, Chen JJ
Blood(2017 Nov)

UBE2O remodels the proteome during terminal erythroid differentiation.
Nguyen AT, Prado MA, Schmidt PJ, Sendamarai AK, Wilson-Grady JT, Min M, Campagna DR, Tian G, Shi Y, Dederer V, Kawan M, Kuehnle N, Paulo JA, Yao Y, Weiss MJ, Justice MJ, Gygi SP, Fleming MD, Finley D
Science (New York, N.Y.)357(6350)(2017 08)

Integrated stress response of vertebrates is regulated by four eIF2? kinases.
Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S
Scientific reports632886(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Heme-Regulated eIF2alpha Kinase Modulates Hepatic FGF21 and Is Activated by PPARbeta/delta Deficiency. Findings suggest that HRI is a potential target for regulating hepatic FGF21 levels.
Title: Heme-Regulated eIF2? Kinase Modulates Hepatic FGF21 and Is Activated by PPAR?/? Deficiency.

These findings indicate that the anti-proliferative actions of PKR on pancreatic beta-cells may contribute to the pathogenesis of type 2 diabetes.
Title: Activation of double-stranded RNA-dependent protein kinase inhibits proliferation of pancreatic ?-cells.

we suggest that Tyr193, Thr485 and Thr490 are essential residues in the catalysis.
Title: Autophosphorylation of heme-regulated eukaryotic initiation factor 2? kinase and the role of the modification in catalysis.

eukaryotic initiation factor 2alpha kinase is a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone.
Title: Hepatic heme-regulated inhibitor (HRI) eukaryotic initiation factor 2alpha kinase: a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone.

The anti-viral properties of eIF2alpha kinases are not always related to their inhibitory effects on host protein synthesis as previously thought and are possibly mediated by phosphorylation of proteins other than eIF2alpha.
Title: The eIF2alpha kinases inhibit vesicular stomatitis virus replication independently of eIF2alpha phosphorylation.

The following Eif2ak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2ak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18622	NM_013557.2 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$363.30 $519.00
OMu81259	XM_017320685.2 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu81259	XM_017320685.1	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2ak1 ( NM_013557.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038585.2 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), mRNA.

Price & Availability↑

CloneID	OMu18622
Clone ID Related Accession (Same CDS sequence)	NM_013557.2
Accession Version	NM_013557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 2-alpha kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB625879.1, AK173160.1 and DV644671.1. On Jan 27, 2007 this sequence version replaced NM_013557.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT 
TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC 
GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG 
TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT 
CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT 
CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA 
GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT 
GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC 
AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC 
ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG 
CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT 
GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC 
CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT 
TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA 
ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CATCACCGGA ACAGCTGGAG 
GGATCCCAGT ACGATGCCAA GTCAGATATG TATAGCTTGG GTGTGATCCT GCTCGAGCTC 
TTTCAGCCAT TCGGGACAGA AATGGAGAGG GCAACAGTCT TAACAGGCGT AAGGACTGGT 
CGGATACCCG AATCCCTCAG TAAAAGGTGT CCGGTGCAAG CCAAGTATAT CCAGCTCCTA 
ACCGGGAGGA ATGTGTCACA GAGACCATCT GCCCTTCAGC TGCTGCAGAG TGAGCTTTTT 
CAAACAACTG GAAATGTTAA TCTCACATTG CAGATGAAGA TAATAGAACA AGAGAAGGAA 
ATTGAAGAAC TAAAGAAGCA ACTAAGCCTC CTTTCTCAGG ACAGAGGGCT GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038585.2
CDS	100..1959
Translation	MLGGSSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVLKEPLQQPTFPFLVANQLLLVSLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCQDNSYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEVRYHLMLHIQMQLCELSLWDWITERNKRSREYVDEAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGTCLYASPEQLEGSQYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLLTGRNVSQRPSALQLLQSELFQTTGNVNLTLQMKIIEQEKEIEELKKQLSLLSQDRGLKR

Target ORF information:

RefSeq Version	NM_013557.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013557.2

ORF Insert Sequence:

ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT 
TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC 
GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG 
TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT 
CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT 
CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA 
GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT 
GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC 
AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC 
ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG 
CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT 
GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC 
CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT 
TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA 
ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CATCACCGGA ACAGCTGGAG 
GGATCCCAGT ACGATGCCAA GTCAGATATG TATAGCTTGG GTGTGATCCT GCTCGAGCTC 
TTTCAGCCAT TCGGGACAGA AATGGAGAGG GCAACAGTCT TAACAGGCGT AAGGACTGGT 
CGGATACCCG AATCCCTCAG TAAAAGGTGT CCGGTGCAAG CCAAGTATAT CCAGCTCCTA 
ACCGGGAGGA ATGTGTCACA GAGACCATCT GCCCTTCAGC TGCTGCAGAG TGAGCTTTTT 
CAAACAACTG GAAATGTTAA TCTCACATTG CAGATGAAGA TAATAGAACA AGAGAAGGAA 
ATTGAAGAAC TAAAGAAGCA ACTAAGCCTC CTTTCTCAGG ACAGAGGGCT GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif2ak1 ( XM_017320685.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176174.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81259
Clone ID Related Accession (Same CDS sequence)	XM_017320685.1 , XM_017320685.2
Accession Version	XM_017320685.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT 
TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC 
GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG 
TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT 
CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT 
CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA 
GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT 
GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC 
AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC 
ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG 
CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT 
GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC 
CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT 
TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA 
ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CATCACCGGA ACAGCTGGAG 
GGATCCCAGT ACGATGCCAA GGTTAATCTC ACATTGCAGA TGAAGATAAT AGAACAAGAG 
AAGGAAATTG AAGAACTAAA GAAGCAACTA AGCCTCCTTT CTCAGGACAG AGGGCTGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176174.1
CDS	98..1723
Translation	MLGGSSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVLKEPLQQPTFPFLVANQLLLVSLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCQDNSYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEVRYHLMLHIQMQLCELSLWDWITERNKRSREYVDEAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGTCLYASPEQLEGSQYDAKVNLTLQMKIIEQEKEIEELKKQLSLLSQDRGLKR

Target ORF information:

RefSeq Version	XM_017320685.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320685.1

ORF Insert Sequence:

ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT 
TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC 
GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG 
TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT 
CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT 
CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA 
GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT 
GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC 
AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC 
ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG 
CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT 
GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC 
CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT 
TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA 
ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CATCACCGGA ACAGCTGGAG 
GGATCCCAGT ACGATGCCAA GGTTAATCTC ACATTGCAGA TGAAGATAAT AGAACAAGAG 
AAGGAAATTG AAGAACTAAA GAAGCAACTA AGCCTCCTTT CTCAGGACAG AGGGCTGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif2ak1 ( XM_017320685.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176174.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81259
Clone ID Related Accession (Same CDS sequence)	XM_017320685.1 , XM_017320685.2
Accession Version	XM_017320685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT 
TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC 
GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG 
TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT 
CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT 
CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA 
GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT 
GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC 
AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC 
ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG 
CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT 
GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC 
CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT 
TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA 
ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CATCACCGGA ACAGCTGGAG 
GGATCCCAGT ACGATGCCAA GGTTAATCTC ACATTGCAGA TGAAGATAAT AGAACAAGAG 
AAGGAAATTG AAGAACTAAA GAAGCAACTA AGCCTCCTTT CTCAGGACAG AGGGCTGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176174.1
CDS	98..1723
Translation	MLGGSSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVLKEPLQQPTFPFLVANQLLLVSLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCQDNSYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEVRYHLMLHIQMQLCELSLWDWITERNKRSREYVDEAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGTCLYASPEQLEGSQYDAKVNLTLQMKIIEQEKEIEELKKQLSLLSQDRGLKR

Target ORF information:

RefSeq Version	XM_017320685.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320685.2

ORF Insert Sequence:

ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT 
TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC 
GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG 
TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT 
CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT 
CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA 
GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT 
GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC 
AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC 
ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG 
CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT 
GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC 
CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT 
TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA 
ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CATCACCGGA ACAGCTGGAG 
GGATCCCAGT ACGATGCCAA GGTTAATCTC ACATTGCAGA TGAAGATAAT AGAACAAGAG 
AAGGAAATTG AAGAACTAAA GAAGCAACTA AGCCTCCTTT CTCAGGACAG AGGGCTGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The eIF2? kinase Heme Regulated Inhibitor (HRI) protects the host from infection by regulating intracellular pathogen trafficking.
Infection and immunity(2018 Jan)
Bahnan W,Boucher JC,Gayle P,Shrestha N,Rosen M,Aktas B,Adkins B,Ager A,Khan WN,Schesser K

HRI coordinates translation by eIF2?P and mTORC1 to mitigate ineffective erythropoiesis in mice during iron deficiency.
Blood(2017 Nov)
Zhang S,Macias-Garcia A,Velazquez J,Paltrinieri E,Kaufman RJ,Chen JJ

UBE2O remodels the proteome during terminal erythroid differentiation.
Science (New York, N.Y.)357(6350)(2017 08)
Nguyen AT,Prado MA,Schmidt PJ,Sendamarai AK,Wilson-Grady JT,Min M,Campagna DR,Tian G,Shi Y,Dederer V,Kawan M,Kuehnle N,Paulo JA,Yao Y,Weiss MJ,Justice MJ,Gygi SP,Fleming MD,Finley D

Integrated stress response of vertebrates is regulated by four eIF2? kinases.
Scientific reports632886(2016 Sep)
Taniuchi S,Miyake M,Tsugawa K,Oyadomari M,Oyadomari S

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Eif2ak1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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