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INS2 cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol INS2
Entrez Gene ID 16334
Full Name insulin II
Synonyms AA986540,Ins-2,InsII,Mody,Mody4
General protein information
Preferred Names

insulin II

Names

insulin-2
proinsulin

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

7

7 F5|7 88.0 cM

Summary This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA Protein Name
NM_008387.5 NP_032413.1 insulin-2 preproprotein
NM_001185084.2 NP_001172013.1 insulin-2 preproprotein
NM_001185083.2 NP_001172012.1 insulin-2 preproprotein



Pan troglodytes (chimpanzee) INS NP_001008996.1
Mus musculus (house mouse) Ins2 NP_032413.1
Gallus gallus (chicken) INS NP_990553.1
Danio rerio (zebrafish) ins NP_571131.1
Xenopus tropicalis (tropical clawed frog) ins NP_001093706.1
Rattus norvegicus (Norway rat) Ins2 NP_062003.1
Canis lupus familiaris (dog) INS NP_001123565.1
Homo sapiens (human) INS NP_000198.1


Related articles in PubMed

The mammal-specific Pdx1 Area II enhancer has multiple essential functions in early endocrine cell specification and postnatal β-cell maturation.
Yang YP, Magnuson MA, Stein R, Wright CV
Development (Cambridge, England)144(2)248-257(2017 Jan)

Genetic evidence that Nkx2.2 acts primarily downstream of Neurog3 in pancreatic endocrine lineage development.
Churchill AJ, Gutiérrez GD, Singer RA, Lorberbaum DS, Fischer KA, Sussel L
eLife6(2017 Jan)

Identification of novel targets of diabetic nephropathy and PEDF peptide treatment using RNA-seq.
Rubin A, Salzberg AC, Imamura Y, Grivitishvilli A, Tombran-Tink J
BMC genomics17(1)936(2016 Nov)

Novel splice variant of mouse insulin2 mRNA: implications for insulin expression.
Panda AC, Kulkarni SD, Muralidharan B, Bakthavachalu B, Seshadri V
FEBS letters584(6)1169-73(2010 Mar)

Increased insulin translation from an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance.
Minn AH, Lan H, Rabaglia ME, Harlan DM, Peculis BA, Attie AD, Shalev A
Molecular endocrinology (Baltimore, Md.)19(3)794-803(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transplantation of transduced hematopoietic stem cells (HSCs) expressing proinsulin II prevents diabetes development.
Title: Inducing Specific Immune Tolerance to Prevent Type 1 Diabetes in NOD Mice.

Data suggest that resveratrol acts on differentiating preadipocytes by inhibiting insulin signaling, mitochondrial biogenesis, and lipogenesis.
Title: Resveratrol inhibits lipogenesis of 3T3-L1 and SGBS cells by inhibition of insulin signaling and mitochondrial mass increase.

Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus.
Title: Wnt3a upregulates brain-derived insulin by increasing NeuroD1 via Wnt/β-catenin signaling in the hypothalamus.

have characterized the distinctive sex-specific phenotypes exhibited by the ApoE(-/-):Ins2(+/Akita) mouse model and present evidence for the action of sex hormones on pancreatic beta-cell function
Title: Sex-Specific Differences in an ApoE(-/-):Ins2(+/Akita) Mouse Model of Accelerated Atherosclerosis.

report that EndMTs occur in the diabetic endothelium of Ins2Akita/wt mouse, and show that induction of sex determining region Y-box 2 (Sox2) is a mediator of excess BMP signaling that results in activation of EndMTs and increased vascular calcification
Title: Endothelial-Mesenchymal Transition in Vascular Calcification of Ins2Akita/+ Mice.

The following INS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu22987 NM_001185083.2
Latest version!
Mus musculus insulin II (Ins2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu22987 NM_008387.5
Latest version!
Mus musculus insulin II (Ins2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu22987 NM_001185084.2
Latest version!
Mus musculus insulin II (Ins2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22987
Clone ID Related Accession (Same CDS sequence) NM_008387.5 , NM_001185084.2 , NM_001185083.2
Accession Version NM_008387.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AK007612.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced NM_008387.4. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP775562.1, AK007612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_032413.1
CDS92..424
Misc Feature(1)11..13
Misc Feature(2)167..421
Misc Feature(3)order(185..187,194..196,200..202,413..415)
Exon (1)1..65
Exon (2)66..278
Exon (3)279..481
Translation

Target ORF information:

RefSeq Version NM_008387.5
Organism Mus musculus(House mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_008387.5

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22987
Clone ID Related Accession (Same CDS sequence) NM_008387.5 , NM_001185084.2 , NM_001185083.2
Accession Version NM_001185084.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AY899305.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced NM_001185084.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP775682.1, AY899305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001172013.1
CDS194..526
Misc Feature(1)125..127
Misc Feature(2)269..523
Misc Feature(3)order(287..289,296..298,302..304,515..517)
Exon (1)1..380
Exon (2)381..583
Translation

Target ORF information:

RefSeq Version NM_001185084.2
Organism Mus musculus(House mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185084.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22987
Clone ID Related Accession (Same CDS sequence) NM_008387.5 , NM_001185084.2 , NM_001185083.2
Accession Version NM_001185083.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced NM_001185083.1. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP775727.1, BG310959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001172012.1
CDS80..412
Misc Feature(1)11..13
Misc Feature(2)155..409
Misc Feature(3)order(173..175,182..184,188..190,401..403)
Exon (1)1..65
Exon (2)66..266
Exon (3)267..469
Translation

Target ORF information:

RefSeq Version NM_001185083.2
Organism Mus musculus(House mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185083.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The mammal-specific Pdx1 Area II enhancer has multiple essential functions in early endocrine cell specification and postnatal β-cell maturation.
Development (Cambridge, England)144(2)248-257(2017 Jan)
Yang YP,Magnuson MA,Stein R,Wright CV


book

Genetic evidence that Nkx2.2 acts primarily downstream of Neurog3 in pancreatic endocrine lineage development.
eLife6(2017 Jan)
Churchill AJ,Gutiérrez GD,Singer RA,Lorberbaum DS,Fischer KA,Sussel L


book

Identification of novel targets of diabetic nephropathy and PEDF peptide treatment using RNA-seq.
BMC genomics17(1)936(2016 Nov)
Rubin A,Salzberg AC,Imamura Y,Grivitishvilli A,Tombran-Tink J


book

Novel splice variant of mouse insulin2 mRNA: implications for insulin expression.
FEBS letters584(6)1169-73(2010 Mar)
Panda AC,Kulkarni SD,Muralidharan B,Bakthavachalu B,Seshadri V


book

Increased insulin translation from an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance.
Molecular endocrinology (Baltimore, Md.)19(3)794-803(2005 Mar)
Minn AH,Lan H,Rabaglia ME,Harlan DM,Peculis BA,Attie AD,Shalev A


 
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