Gene Symbol | Usp33 |
Entrez Gene ID | 170822 |
Full Name | ubiquitin specific peptidase 33 |
Synonyms | 9830169D19Rik,AA409780,Vdu1 |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Usp33 cDNA ORF clone
gRNAs
Gene Symbol | Usp33 |
Entrez Gene ID | 170822 |
Full Name | ubiquitin specific peptidase 33 |
Synonyms | 9830169D19Rik,AA409780,Vdu1 |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
NM_133247.3 | NP_573510.2 | ubiquitin carboxyl-terminal hydrolase 33 isoform 1 |
NM_001076676.2 | NP_001070144.1 | ubiquitin carboxyl-terminal hydrolase 33 isoform 2 |
NM_001355666.1 | NP_001342595.1 | ubiquitin carboxyl-terminal hydrolase 33 isoform 4 |
XM_030252443.1 | XP_030108303.1 | ubiquitin carboxyl-terminal hydrolase 33 isoform X1 |
XM_030252444.1 | XP_030108304.1 | ubiquitin carboxyl-terminal hydrolase 33 isoform X2 |
XM_006501079.2 | XP_006501142.1 | ubiquitin carboxyl-terminal hydrolase 33 isoform X1 |
NM_001252486.1 | NP_001239415.1 | ubiquitin carboxyl-terminal hydrolase 33 isoform 3 |
Pan troglodytes (chimpanzee) | USP33 | XP_003308213.1 |
LOC710693 | XP_002808266.1 | |
Xenopus tropicalis (tropical clawed frog) | usp33 | NP_001016228.1 |
Mus musculus (house mouse) | Usp33 | NP_573510.2 |
Homo sapiens (human) | USP33 | NP_055832.3 |
Rattus norvegicus (Norway rat) | Usp33 | NP_001178023.1 |
Danio rerio (zebrafish) | usp33 | NP_998392.1 |
Bos taurus (cattle) | USP33 | NP_001094609.1 |
Canis lupus familiaris (dog) | USP33 | XP_005622106.1 |
Gallus gallus (chicken) | USP33 | XP_004936727.1 |
The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination.
Cubillos-Rojas M, Schneider T, Hadjebi O, Pedrazza L, de Oliveira JR, Langa F, Gu?net JL, Duran J, de Anta JM, Alc?ntara S, Ruiz R, P?rez-Villegas EM, Aguilar-Montilla FJ, Carri?n ?M, Armengol JA, Baple E, Crosby AH, Bartrons R, Ventura F, Rosa JL
Oncotarget7(35)56083-56106(2016 Aug)
Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.
Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
The Journal of biological chemistry289(28)19789-98(2014 Jul)
Midline crossing and Slit responsiveness of commissural axons require USP33.
Yuasa-Kawada J, Kinoshita-Kawada M, Wu G, Rao Y, Wu JY
Nature neuroscience12(9)1087-9(2009 Sep)
Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration.
Yuasa-Kawada J, Kinoshita-Kawada M, Rao Y, Wu JY
Proceedings of the National Academy of Sciences of the United States of America106(34)14530-5(2009 Aug)
Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.
Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G
The Journal of biological chemistry277(7)4656-62(2002 Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Knockdown or chemical inhibition of p97 causes robust accumulation of USP33 due to inhibition of its degradation
Title: Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.
Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling
Title: Midline crossing and Slit responsiveness of commissural axons require USP33.
The following Usp33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu01227 | |
Clone ID Related Accession (Same CDS sequence) | NM_001076676.2 | |
Accession Version | NM_001076676.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-22 | |
Organism | Mus musculus(house mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087467.1, AK082943.1 and AA210301.1. On Nov 16, 2011 this sequence version replaced NM_001076676.1. Transcript Variant: This variant (2) uses two alternate in-frame splice junctions compared to variant 1, resulting in a shorter protein (isoform 2). ##Evidence-Data-START## Transcript exon combination :: AK082251.1, AK082943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001070144.1 |
CDS | 214..2907 |
Translation |
Target ORF information:
Target ORF information:
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu05115 | |
Clone ID Related Accession (Same CDS sequence) | NM_001252486.1 | |
Accession Version | NM_001252486.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-22 | |
Organism | Mus musculus(house mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform 3 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087467.1, AK143844.1, BC089315.1 and AA210301.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1, resulting in a shorter protein (isoform 3). ##Evidence-Data-START## Transcript exon combination :: AK087467.1, SRR1660811.179005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001239415.1 |
CDS | 214..2919 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu09982 | |
Clone ID Related Accession (Same CDS sequence) | NM_133247.3 , XM_030252443.1 | |
Accession Version | NM_133247.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-22 | |
Organism | Mus musculus(house mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087467.1, AK143844.1, BC089315.1 and AA210301.1. On Nov 16, 2011 this sequence version replaced NM_133247.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF383174.1, SRR1660817.16866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_573510.2 |
CDS | 214..2943 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31674 | |
Clone ID Related Accession (Same CDS sequence) | NM_001355666.1 , XM_006501079.2 | |
Accession Version | NM_001355666.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-25 | |
Organism | Mus musculus(house mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform 4 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122529.5 and AA210301.1. On Sep 23, 2017 this sequence version replaced XM_006501079.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.8945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001342595.1 |
CDS | 214..2532 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31674 | |
Clone ID Related Accession (Same CDS sequence) | NM_001355666.1 , XM_006501079.2 | |
Accession Version | XM_006501079.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(House mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform X1 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006501142.1 |
CDS | 208..2526 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu09982 | |
Clone ID Related Accession (Same CDS sequence) | NM_133247.3 , XM_030252443.1 | |
Accession Version | XM_030252443.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_030108303.1 |
CDS | 293..3022 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu88165 | |
Clone ID Related Accession (Same CDS sequence) | XM_030252444.1 | |
Accession Version | XM_030252444.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | ubiquitin carboxyl-terminal hydrolase 33 isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_030108304.1 |
CDS | 508..2802 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination. |
Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2. |
Midline crossing and Slit responsiveness of commissural axons require USP33. |
Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration. |
Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein. |