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Melk cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Melk
Entrez Gene ID 17279
Full Name maternal embryonic leucine zipper kinase
Synonyms AI327312,MPK38,mKIAA0175
General protein information
Preferred Names

maternal embryonic leucine zipper kinase

Names

maternal embryonic leucine zipper kinase
protein kinase PK38
tyrosine-protein kinase MELK
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

4

4 B1|4 23.46 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006537640.3 XP_006537703.1 maternal embryonic leucine zipper kinase isoform X1
NM_010790.2 NP_034920.2 maternal embryonic leucine zipper kinase
XM_006537640.2 XP_006537703.1 maternal embryonic leucine zipper kinase isoform X1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) MELK XP_538730.2
Gallus gallus (chicken) MELK NP_001026680.1
Xenopus tropicalis (tropical clawed frog) melk NP_001016390.1
Rattus norvegicus (Norway rat) Melk NP_001102132.1
Pan troglodytes (chimpanzee) MELK XP_001169038.1
Mus musculus (house mouse) Melk NP_034920.2
Danio rerio (zebrafish) melk NP_996771.2
Arabidopsis thaliana (thale cress) CIPK7 NP_188940.1
Homo sapiens (human) MELK NP_055606.1
MELK XP_001115076.2
Bos taurus (cattle) MELK NP_001104730.1
Caenorhabditis elegans (roundworm) pig-1 NP_001023420.2
Arabidopsis thaliana (thale cress) CIPK4 NP_193194.1

Gene Ontology


Bibliography

Related articles in PubMed

Maternal embryonic leucine zipper kinase regulates pancreatic ductal, but not ?-cell, regeneration.
Chung CH, Miller A, Panopoulos A, Hao E, Margolis R, Terskikh A, Levine F
Physiological reports2(9)(2014 Sep)

Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway.
Du T, Qu Y, Li J, Li H, Su L, Zhou Q, Yan M, Li C, Zhu Z, Liu B
Molecular cancer13100(2014 May)

MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells.
Wang Y, Lee YM, Baitsch L, Huang A, Xiang Y, Tong H, Lako A, Von T, Choi C, Lim E, Min J, Li L, Stegmeier F, Schlegel R, Eck MJ, Gray NS, Mitchison TJ, Zhao JJ
eLife3e01763(2014 May)

The structures of the kinase domain and UBA domain of MPK38 suggest the activation mechanism for kinase activity.
Cho YS, Yoo J, Park S, Cho HS
Acta crystallographica. Section D, Biological crystallography70(Pt 2)514-21(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MELK promotes cell migration and invasion via the FAK/Paxillin pathway, and plays an important role in the occurrence and development of gastric cancer.
Title: Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway.

Phosphorylation of the activation loop of MPK38 induces its movement resulting in kinase activation.
Title: The structures of the kinase domain and UBA domain of MPK38 suggest the activation mechanism for kinase activity.

These results indicate that Trx functions as a physiological inhibitor of MPK38, which plays an important role in inducing ASK1-, TGF-beta, and p53-mediated activity
Title: Thioredoxin inhibits MPK38-induced ASK1, TGF-?, and p53 function in a phosphorylation-dependent manner.

MPK38 may act as a novel regulator for promoting p53 activity through direct phosphorylation of p53 at Ser(15).
Title: Murine protein serine-threonine kinase 38 activates p53 function through Ser15 phosphorylation.

an important role for MPK38-mediated phosphorylation of PDK1 in the negative regulation of PDK1 activity.
Title: PDK1 protein phosphorylation at Thr354 by murine protein serine-threonine kinase 38 contributes to negative regulation of PDK1 protein activity.

The following Melk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Melk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu06905 NM_010790.2
Latest version!		 Mus musculus maternal embryonic leucine zipper kinase (Melk), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $377.30
$539.00
OMu32737 XM_006537640.3
Latest version!		 Mus musculus maternal embryonic leucine zipper kinase (Melk), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu32737 XM_006537640.2 Mus musculus maternal embryonic leucine zipper kinase (Melk), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu06905
Clone ID Related Accession (Same CDS sequence) NM_010790.2
Accession Version NM_010790.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-10
Organism Mus musculus(house mouse)
Product maternal embryonic leucine zipper kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK011932.1. On Jun 19, 2003 this sequence version replaced NM_010790.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK011932.1, AK137000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## 

1
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ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 
ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 
CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 
AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 
GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 
GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 
CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 
GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGAGAG GGAAATACGA AGTTCCTAAG TGGCTCTCTC CCAGTAGCAT TCTGCTTCTC 
CAGCAGATGT TGCAGGTGGA CCCAAAGAAA CGGATTTCTA TGAGAAATCT CCTGAACCAT 
CCCTGGGTCA TGCAAGATTA CAGCTGTCCC GTGGAGTGGC AAAGCAAGAC TCCTTTGACT 
CACCTCGATG AGGATTGCGT GACAGAGCTT TCTGTACATC ACCGCAGCAG CAGGCAGACA 
ATGGAGGATT TAATTTCGTC GTGGCAGTAC GATCACCTCA CAGCCACCTA CCTTCTGCTT 
CTAGCCAAGA AGGCCCGGGG GAAGCCGGCT CGTCTACAGC TCCTGTCCTT CTCTTGTGGA 
ACCGCCAGCA CCACTCCAAA GTCAAAGAAT CTGAGCCTGG AAGATATGAG CACAAGTGAT 
GATAACTGTG TGGCTGGATT GATAGACTAT GAATTGTGTG AAGATAAATT ATTAGCTCCC 
AAGACGCCAC AGGTTACCAA ACACTTGGCA GAATCAAATC ACGCAGCATC TAAATCACCA 
GCGCCAGGGG TACGCAGAGC AGTGGCAAAT AAATTAATGG ACAAAGAAAA TGTGTGCACT 
CCCAAGTCTT CTGTGAAGAA TGAAGAGCAG TTTGTATTTT CTGAGCCGAA GATTCCAGTT 
AGTAAGAACC AGTATAAGAG AGAAATACCC GCCTCACCAA CCCGTTTCCC AACACCTGCA 
AAAGCTAGAG CCCAGTGCCT GAGAGAAGCC CCGGTTAGAA CACCAGGGAA TTCCGCAGGA 
GCAGACACAC TAACGACAGG TGTCATTAGC CCCGAGAGGA GGTGCCGTTC AATGGACGTG 
GATCTCAACC AGGCACACAT GGAGGATACC CCGAAAAAGA AAGGAACCAA TGTGTTTGGG 
AGCCTTGAGA GAGGACTGGA TAAGGTTCTC ACTGCGCTCA CAAGGAACAA GAAGAAGGGC 
TCTGCCAGAG ATGGACCAAG AAAGCGAAAG CTGCACTACA ATGTGACTAC AACTCGCCTG 
GTGAACCCCG ACCAGCTCCT GAGCGAAATC ATGGCTATTC TTCCAAAGAA GAACGTGGAC 
TTCGTACAGA AAGGTTACAC TCTAAAGTGT CAAACGCAGT CTGATTTTGG CAAAGTGACA 
ATGCAGTTTG AACTGGAAGT GTGCCAGCTG CAGAGACCTG ACGTGGTAGG CATCCGGAGA 
CAGCGGCTGA AGGGTGATGC CTGGGTTTAC AAGAGATTAG TGGAAGATAT CTTGTCTGGC 
TGCAAGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_034920.2
CDS195..2126
Translation

Target ORF information:

RefSeq Version NM_010790.2
Organism Mus musculus(house mouse)
Definition Mus musculus maternal embryonic leucine zipper kinase (Melk), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_010790.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 
ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 
CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 
AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 
GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 
GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 
CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 
GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGAGAG GGAAATACGA AGTTCCTAAG TGGCTCTCTC CCAGTAGCAT TCTGCTTCTC 
CAGCAGATGT TGCAGGTGGA CCCAAAGAAA CGGATTTCTA TGAGAAATCT CCTGAACCAT 
CCCTGGGTCA TGCAAGATTA CAGCTGTCCC GTGGAGTGGC AAAGCAAGAC TCCTTTGACT 
CACCTCGATG AGGATTGCGT GACAGAGCTT TCTGTACATC ACCGCAGCAG CAGGCAGACA 
ATGGAGGATT TAATTTCGTC GTGGCAGTAC GATCACCTCA CAGCCACCTA CCTTCTGCTT 
CTAGCCAAGA AGGCCCGGGG GAAGCCGGCT CGTCTACAGC TCCTGTCCTT CTCTTGTGGA 
ACCGCCAGCA CCACTCCAAA GTCAAAGAAT CTGAGCCTGG AAGATATGAG CACAAGTGAT 
GATAACTGTG TGGCTGGATT GATAGACTAT GAATTGTGTG AAGATAAATT ATTAGCTCCC 
AAGACGCCAC AGGTTACCAA ACACTTGGCA GAATCAAATC ACGCAGCATC TAAATCACCA 
GCGCCAGGGG TACGCAGAGC AGTGGCAAAT AAATTAATGG ACAAAGAAAA TGTGTGCACT 
CCCAAGTCTT CTGTGAAGAA TGAAGAGCAG TTTGTATTTT CTGAGCCGAA GATTCCAGTT 
AGTAAGAACC AGTATAAGAG AGAAATACCC GCCTCACCAA CCCGTTTCCC AACACCTGCA 
AAAGCTAGAG CCCAGTGCCT GAGAGAAGCC CCGGTTAGAA CACCAGGGAA TTCCGCAGGA 
GCAGACACAC TAACGACAGG TGTCATTAGC CCCGAGAGGA GGTGCCGTTC AATGGACGTG 
GATCTCAACC AGGCACACAT GGAGGATACC CCGAAAAAGA AAGGAACCAA TGTGTTTGGG 
AGCCTTGAGA GAGGACTGGA TAAGGTTCTC ACTGCGCTCA CAAGGAACAA GAAGAAGGGC 
TCTGCCAGAG ATGGACCAAG AAAGCGAAAG CTGCACTACA ATGTGACTAC AACTCGCCTG 
GTGAACCCCG ACCAGCTCCT GAGCGAAATC ATGGCTATTC TTCCAAAGAA GAACGTGGAC 
TTCGTACAGA AAGGTTACAC TCTAAAGTGT CAAACGCAGT CTGATTTTGG CAAAGTGACA 
ATGCAGTTTG AACTGGAAGT GTGCCAGCTG CAGAGACCTG ACGTGGTAGG CATCCGGAGA 
CAGCGGCTGA AGGGTGATGC CTGGGTTTAC AAGAGATTAG TGGAAGATAT CTTGTCTGGC 
TGCAAGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu32737
Clone ID Related Accession (Same CDS sequence) XM_006537640.3 , XM_006537640.2
Accession Version XM_006537640.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 
ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 
CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 
AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 
GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 
GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 
CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 
GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGTTGA CTCACCTCGA TGAGGATTGC GTGACAGAGC TTTCTGTACA TCACCGCAGC 
AGCAGGCAGA CAATGGAGGA TTTAATTTCG TCGTGGCAGT ACGATCACCT CACAGCCACC 
TACCTTCTGC TTCTAGCCAA GAAGGCCCGG GGGAAGCCGG CTCGTCTACA GCTCCTGTCC 
TTCTCTTGTG GAACCGCCAG CACCACTCCA AAGTCAAAGA ATCTGAGCCT GGAAGATATG 
AGCACAAGTG ATGATAACTG TGTGGCTGGA TTGATAGACT ATGAATTGTG TGAAGATAAA 
TTATTAGCTC CCAAGACGCC ACAGGTTACC AAACACTTGG CAGAATCAAA TCACGCAGCA 
TCTAAATCAC CAGCGCCAGG GGTACGCAGA GCAGTGGCAA ATAAATTAAT GGACAAAGAA 
AATGTGTGCA CTCCCAAGTC TTCTGTGAAG AATGAAGAGC AGTTTGTATT TTCTGAGCCG 
AAGATTCCAG TTAGTAAGAA CCAGTATAAG AGAGAAATAC CCGCCTCACC AACCCGTTTC 
CCAACACCTG CAAAAGCTAG AGCCCAGTGC CTGAGAGAAG CCCCGGTTAG AACACCAGGG 
AATTCCGCAG GAGCAGACAC ACTAACGACA GGTGTCATTA GCCCCGAGAG GAGGTGCCGT 
TCAATGGACG TGGATCTCAA CCAGGCACAC ATGGAGGATA CCCCGAAAAA GAAAGGAACC 
AATGTGTTTG GGAGCCTTGA GAGAGGACTG GATAAGGTTC TCACTGCGCT CACAAGGAAC 
AAGAAGAAGG GCTCTGCCAG AGATGGACCA AGAAAGCGAA AGCTGCACTA CAATGTGACT 
ACAACTCGCC TGGTGAACCC CGACCAGCTC CTGAGCGAAA TCATGGCTAT TCTTCCAAAG 
AAGAACGTGG ACTTCGTACA GAAAGGTTAC ACTCTAAAGT GTCAAACGCA GTCTGATTTT 
GGCAAAGTGA CAATGCAGTT TGAACTGGAA GTGTGCCAGC TGCAGAGACC TGACGTGGTA 
GGCATCCGGA GACAGCGGCT GAAGGGTGAT GCCTGGGTTT ACAAGAGATT AGTGGAAGAT 
ATCTTGTCTG GCTGCAAGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006537703.1
CDS236..1999
Translation

Target ORF information:

RefSeq Version XM_006537640.2
Organism Mus musculus(house mouse)
Definition Mus musculus maternal embryonic leucine zipper kinase (Melk), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006537640.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 
ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 
CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 
AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 
GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 
GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 
CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 
GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGTTGA CTCACCTCGA TGAGGATTGC GTGACAGAGC TTTCTGTACA TCACCGCAGC 
AGCAGGCAGA CAATGGAGGA TTTAATTTCG TCGTGGCAGT ACGATCACCT CACAGCCACC 
TACCTTCTGC TTCTAGCCAA GAAGGCCCGG GGGAAGCCGG CTCGTCTACA GCTCCTGTCC 
TTCTCTTGTG GAACCGCCAG CACCACTCCA AAGTCAAAGA ATCTGAGCCT GGAAGATATG 
AGCACAAGTG ATGATAACTG TGTGGCTGGA TTGATAGACT ATGAATTGTG TGAAGATAAA 
TTATTAGCTC CCAAGACGCC ACAGGTTACC AAACACTTGG CAGAATCAAA TCACGCAGCA 
TCTAAATCAC CAGCGCCAGG GGTACGCAGA GCAGTGGCAA ATAAATTAAT GGACAAAGAA 
AATGTGTGCA CTCCCAAGTC TTCTGTGAAG AATGAAGAGC AGTTTGTATT TTCTGAGCCG 
AAGATTCCAG TTAGTAAGAA CCAGTATAAG AGAGAAATAC CCGCCTCACC AACCCGTTTC 
CCAACACCTG CAAAAGCTAG AGCCCAGTGC CTGAGAGAAG CCCCGGTTAG AACACCAGGG 
AATTCCGCAG GAGCAGACAC ACTAACGACA GGTGTCATTA GCCCCGAGAG GAGGTGCCGT 
TCAATGGACG TGGATCTCAA CCAGGCACAC ATGGAGGATA CCCCGAAAAA GAAAGGAACC 
AATGTGTTTG GGAGCCTTGA GAGAGGACTG GATAAGGTTC TCACTGCGCT CACAAGGAAC 
AAGAAGAAGG GCTCTGCCAG AGATGGACCA AGAAAGCGAA AGCTGCACTA CAATGTGACT 
ACAACTCGCC TGGTGAACCC CGACCAGCTC CTGAGCGAAA TCATGGCTAT TCTTCCAAAG 
AAGAACGTGG ACTTCGTACA GAAAGGTTAC ACTCTAAAGT GTCAAACGCA GTCTGATTTT 
GGCAAAGTGA CAATGCAGTT TGAACTGGAA GTGTGCCAGC TGCAGAGACC TGACGTGGTA 
GGCATCCGGA GACAGCGGCT GAAGGGTGAT GCCTGGGTTT ACAAGAGATT AGTGGAAGAT 
ATCTTGTCTG GCTGCAAGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu32737
Clone ID Related Accession (Same CDS sequence) XM_006537640.3 , XM_006537640.2
Accession Version XM_006537640.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 
ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 
CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 
AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 
GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 
GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 
CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 
GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGTTGA CTCACCTCGA TGAGGATTGC GTGACAGAGC TTTCTGTACA TCACCGCAGC 
AGCAGGCAGA CAATGGAGGA TTTAATTTCG TCGTGGCAGT ACGATCACCT CACAGCCACC 
TACCTTCTGC TTCTAGCCAA GAAGGCCCGG GGGAAGCCGG CTCGTCTACA GCTCCTGTCC 
TTCTCTTGTG GAACCGCCAG CACCACTCCA AAGTCAAAGA ATCTGAGCCT GGAAGATATG 
AGCACAAGTG ATGATAACTG TGTGGCTGGA TTGATAGACT ATGAATTGTG TGAAGATAAA 
TTATTAGCTC CCAAGACGCC ACAGGTTACC AAACACTTGG CAGAATCAAA TCACGCAGCA 
TCTAAATCAC CAGCGCCAGG GGTACGCAGA GCAGTGGCAA ATAAATTAAT GGACAAAGAA 
AATGTGTGCA CTCCCAAGTC TTCTGTGAAG AATGAAGAGC AGTTTGTATT TTCTGAGCCG 
AAGATTCCAG TTAGTAAGAA CCAGTATAAG AGAGAAATAC CCGCCTCACC AACCCGTTTC 
CCAACACCTG CAAAAGCTAG AGCCCAGTGC CTGAGAGAAG CCCCGGTTAG AACACCAGGG 
AATTCCGCAG GAGCAGACAC ACTAACGACA GGTGTCATTA GCCCCGAGAG GAGGTGCCGT 
TCAATGGACG TGGATCTCAA CCAGGCACAC ATGGAGGATA CCCCGAAAAA GAAAGGAACC 
AATGTGTTTG GGAGCCTTGA GAGAGGACTG GATAAGGTTC TCACTGCGCT CACAAGGAAC 
AAGAAGAAGG GCTCTGCCAG AGATGGACCA AGAAAGCGAA AGCTGCACTA CAATGTGACT 
ACAACTCGCC TGGTGAACCC CGACCAGCTC CTGAGCGAAA TCATGGCTAT TCTTCCAAAG 
AAGAACGTGG ACTTCGTACA GAAAGGTTAC ACTCTAAAGT GTCAAACGCA GTCTGATTTT 
GGCAAAGTGA CAATGCAGTT TGAACTGGAA GTGTGCCAGC TGCAGAGACC TGACGTGGTA 
GGCATCCGGA GACAGCGGCT GAAGGGTGAT GCCTGGGTTT ACAAGAGATT AGTGGAAGAT 
ATCTTGTCTG GCTGCAAGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006537703.1
CDS203..1966
Translation

Target ORF information:

RefSeq Version XM_006537640.3
Organism Mus musculus(house mouse)
Definition Mus musculus maternal embryonic leucine zipper kinase (Melk), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006537640.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 
GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 
ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 
CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 
AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 
CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 
GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 
GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 
AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 
CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 
GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 
ATAATGTTGA CTCACCTCGA TGAGGATTGC GTGACAGAGC TTTCTGTACA TCACCGCAGC 
AGCAGGCAGA CAATGGAGGA TTTAATTTCG TCGTGGCAGT ACGATCACCT CACAGCCACC 
TACCTTCTGC TTCTAGCCAA GAAGGCCCGG GGGAAGCCGG CTCGTCTACA GCTCCTGTCC 
TTCTCTTGTG GAACCGCCAG CACCACTCCA AAGTCAAAGA ATCTGAGCCT GGAAGATATG 
AGCACAAGTG ATGATAACTG TGTGGCTGGA TTGATAGACT ATGAATTGTG TGAAGATAAA 
TTATTAGCTC CCAAGACGCC ACAGGTTACC AAACACTTGG CAGAATCAAA TCACGCAGCA 
TCTAAATCAC CAGCGCCAGG GGTACGCAGA GCAGTGGCAA ATAAATTAAT GGACAAAGAA 
AATGTGTGCA CTCCCAAGTC TTCTGTGAAG AATGAAGAGC AGTTTGTATT TTCTGAGCCG 
AAGATTCCAG TTAGTAAGAA CCAGTATAAG AGAGAAATAC CCGCCTCACC AACCCGTTTC 
CCAACACCTG CAAAAGCTAG AGCCCAGTGC CTGAGAGAAG CCCCGGTTAG AACACCAGGG 
AATTCCGCAG GAGCAGACAC ACTAACGACA GGTGTCATTA GCCCCGAGAG GAGGTGCCGT 
TCAATGGACG TGGATCTCAA CCAGGCACAC ATGGAGGATA CCCCGAAAAA GAAAGGAACC 
AATGTGTTTG GGAGCCTTGA GAGAGGACTG GATAAGGTTC TCACTGCGCT CACAAGGAAC 
AAGAAGAAGG GCTCTGCCAG AGATGGACCA AGAAAGCGAA AGCTGCACTA CAATGTGACT 
ACAACTCGCC TGGTGAACCC CGACCAGCTC CTGAGCGAAA TCATGGCTAT TCTTCCAAAG 
AAGAACGTGG ACTTCGTACA GAAAGGTTAC ACTCTAAAGT GTCAAACGCA GTCTGATTTT 
GGCAAAGTGA CAATGCAGTT TGAACTGGAA GTGTGCCAGC TGCAGAGACC TGACGTGGTA 
GGCATCCGGA GACAGCGGCT GAAGGGTGAT GCCTGGGTTT ACAAGAGATT AGTGGAAGAT 
ATCTTGTCTG GCTGCAAGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Maternal embryonic leucine zipper kinase regulates pancreatic ductal, but not ?-cell, regeneration.
Physiological reports2(9)(2014 Sep)
Chung CH,Miller A,Panopoulos A,Hao E,Margolis R,Terskikh A,Levine F


book

Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway.
Molecular cancer13100(2014 May)
Du T,Qu Y,Li J,Li H,Su L,Zhou Q,Yan M,Li C,Zhu Z,Liu B


book

MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells.
eLife3e01763(2014 May)
Wang Y,Lee YM,Baitsch L,Huang A,Xiang Y,Tong H,Lako A,Von T,Choi C,Lim E,Min J,Li L,Stegmeier F,Schlegel R,Eck MJ,Gray NS,Mitchison TJ,Zhao JJ


book

The structures of the kinase domain and UBA domain of MPK38 suggest the activation mechanism for kinase activity.
Acta crystallographica. Section D, Biological crystallography70(Pt 2)514-21(2014 Feb)
Cho YS,Yoo J,Park S,Cho HS