ORF cDNA Clones and Custom Clones » Species Summary » Mus musculus » Pctp cDNA ORF clone

Pctp cDNA ORF clone, Mus musculus(house mouse)

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Summary

Gene Symbol	Pctp
Entrez Gene ID	18559
Full Name	phosphatidylcholine transfer protein
Synonyms	PC-TP,StarD2
General protein information	Preferred Names phosphatidylcholine transfer protein Names phosphatidylcholine transfer protein START domain-containing protein 2 stAR-related lipid transfer protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 C\|11 54.7 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_008796.3	NP_032822.2	phosphatidylcholine transfer protein isoform 1
NM_001316372.1	NP_001303301.1	phosphatidylcholine transfer protein isoform 2

Homologies

	PCTP	XP_001104194.1
Mus musculus (house mouse)	Pctp	NP_032822.2
Homo sapiens (human)	PCTP	NP_067036.2
Canis lupus familiaris (dog)	PCTP	XP_537685.2
Rattus norvegicus (Norway rat)	Pctp	NP_058921.1
Gallus gallus (chicken)	PCTP	XP_415652.1
Bos taurus (cattle)	PCTP	NP_777260.1
Pan troglodytes (chimpanzee)	PCTP	XP_511897.3
Danio rerio (zebrafish)	pctp	NP_001124258.1

Bibliography

Related articles in PubMed

Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress.
Ersoy BA, Maner-Smith KM, Li Y, Alpertunga I, Cohen DE
The Journal of clinical investigation128(1)141-156(2018 Jan)

Phosphatidylcholine transfer protein/StarD2 promotes microvesicular steatosis and liver injury in murine experimental steatohepatitis.
Nicholls HT, Hornick JL, Cohen DE
American journal of physiology. Gastrointestinal and liver physiology313(1)G50-G61(2017 Jul)

Genetic ablation of phosphatidylcholine transfer protein/StarD2 in ob/ob mice improves glucose tolerance without increasing energy expenditure.
Krisko TI, LeClair KB, Cohen DE
Metabolism: clinical and experimental68145-149(2017 03)

Thioesterase superfamily member 2 (Them2) and phosphatidylcholine transfer protein (PC-TP) interact to promote fatty acid oxidation and control glucose utilization.
Kawano Y, Ersoy BA, Li Y, Nishiumi S, Yoshida M, Cohen DE
Molecular and cellular biology34(13)2396-408(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations suggest that PC-TP promotes liver injury by mediating the intermembrane transfer of phosphatidylcholines, thus stabilizing more pathogenic microvesicular lipid droplets.
Title: Phosphatidylcholine transfer protein/StarD2 promotes microvesicular steatosis and liver injury in murine experimental steatohepatitis.

Improved glucose tolerance in Pctp(-/-);ob/ob mice in the absence of increases in energy expenditure or core body temperature indicates a direct pathogenic role for Phosphatidylcholine transfer protein in diabetes in leptin deficient mice
Title: Genetic ablation of phosphatidylcholine transfer protein/StarD2 in ob/ob mice improves glucose tolerance without increasing energy expenditure.

Them2 and phosphatidylcholine transfer protein regulate lipid and glucose metabolism
Title: Thioesterase superfamily member 2 (Them2) and phosphatidylcholine transfer protein (PC-TP) interact to promote fatty acid oxidation and control glucose utilization.

PC-TP-THEM2 complex reduces phosphorylation and stability of IRS2 and increases stability of the TSC1-TSC2 complex
Title: Phosphatidylcholine transfer protein interacts with thioesterase superfamily member 2 to attenuate insulin signaling.

Findings support a key role for Pctp in limiting mitochondrial oxidation of fatty acids and thus regulating adaptive thermogenesis in BAT.
Title: Mice lacking Pctp /StarD2 exhibit increased adaptive thermogenesis and enlarged mitochondria in brown adipose tissue.

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The following Pctp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pctp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20728	NM_008796.3 Latest version!	Mus musculus phosphatidylcholine transfer protein (Pctp), transcript variant 1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$188.30 $269.00
OMu45714	NM_001316372.1 Latest version!	Mus musculus phosphatidylcholine transfer protein (Pctp), transcript variant 2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pctp ( NM_008796.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032822.2 Mus musculus phosphatidylcholine transfer protein (Pctp), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20728
Clone ID Related Accession (Same CDS sequence)	NM_008796.3
Accession Version	NM_008796.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	phosphatidylcholine transfer protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY144797.1, BC071234.1, BB614866.1 and AL645695.18. On Oct 20, 2015 this sequence version replaced NM_008796.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053478.1, SRR1660819.127082.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CCGCATGCTG CTTCTCGGAC GAGCAGTTCC GGGAGGCTTG TGCTGAACTC 
CAGAAACCGG CGCTGACCGG GGCCGATTGG CAGCTCCTGG TAGAGGCCTC AGGCATAACC 
ATCTACCGGC TGCTGGACCA GCCAAGTGGA CTTTATGAGT ATAAGGTGTT CGGTGTCCTG 
GAAGGCTGCT CGCCGGCTCT CCTCGCAGAT GTTTACATGG ACTTAGACTA CAGAAAACAA 
TGGGACCAGT ATGTGAAAGA ACTCTACGAG AAGGAGAGTG ATGAACAGAT GGTGGCATAC 
TGGGAAGTGA AGTACCCTTT CCCGCTGTCC AACAGAGATT ACGTCTACAC CCGCCAGCGC 
CGAGACCTGG ATGTGGACGG GAGGAAGATC TACGTGGTCC TGGCCCAGAG TATCTCGGCA 
CCTCAGTTTC CCGAGAAGTC TGGGGTCATC CGAGTGAAAC AGTACAAACA GAGCCTGGCG 
ATCGAGAGTG ACGGCAAGAA GGGGAGCAGA GTTTTCATGT ACTACTTTGA TAACCCGGGT 
GGCCAAATTC CGTCCTGGCT CATTAACTGG GCAGCCAAGA ATGGAGTTCC AAACTTTTTG 
AAGGACATGG TGAAAGCGTG TCAGAACTAC CACAAGAAAA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032822.2
CDS	53..697
Translation	MAGAACCFSDEQFREACAELQKPALTGADWQLLVEASGITIYRLLDQPSGLYEYKVFGVLEGCSPALLADVYMDLDYRKQWDQYVKELYEKESDEQMVAYWEVKYPFPLSNRDYVYTRQRRDLDVDGRKIYVVLAQSISAPQFPEKSGVIRVKQYKQSLAIESDGKKGSRVFMYYFDNPGGQIPSWLINWAAKNGVPNFLKDMVKACQNYHKKT

Target ORF information:

RefSeq Version	NM_008796.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylcholine transfer protein (Pctp), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008796.3

ORF Insert Sequence:

ATGGCGGGGG CCGCATGCTG CTTCTCGGAC GAGCAGTTCC GGGAGGCTTG TGCTGAACTC 
CAGAAACCGG CGCTGACCGG GGCCGATTGG CAGCTCCTGG TAGAGGCCTC AGGCATAACC 
ATCTACCGGC TGCTGGACCA GCCAAGTGGA CTTTATGAGT ATAAGGTGTT CGGTGTCCTG 
GAAGGCTGCT CGCCGGCTCT CCTCGCAGAT GTTTACATGG ACTTAGACTA CAGAAAACAA 
TGGGACCAGT ATGTGAAAGA ACTCTACGAG AAGGAGAGTG ATGAACAGAT GGTGGCATAC 
TGGGAAGTGA AGTACCCTTT CCCGCTGTCC AACAGAGATT ACGTCTACAC CCGCCAGCGC 
CGAGACCTGG ATGTGGACGG GAGGAAGATC TACGTGGTCC TGGCCCAGAG TATCTCGGCA 
CCTCAGTTTC CCGAGAAGTC TGGGGTCATC CGAGTGAAAC AGTACAAACA GAGCCTGGCG 
ATCGAGAGTG ACGGCAAGAA GGGGAGCAGA GTTTTCATGT ACTACTTTGA TAACCCGGGT 
GGCCAAATTC CGTCCTGGCT CATTAACTGG GCAGCCAAGA ATGGAGTTCC AAACTTTTTG 
AAGGACATGG TGAAAGCGTG TCAGAACTAC CACAAGAAAA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pctp ( NM_001316372.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001303301.1 Mus musculus phosphatidylcholine transfer protein (Pctp), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu45714
Clone ID Related Accession (Same CDS sequence)	NM_001316372.1
Accession Version	NM_001316372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	phosphatidylcholine transfer protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY144797.1, BC071234.1 and AL645695.18. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071234.1, CD564182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CCGCATGCTG CTTCTCGGAC GAGCAGTTCC GGGAGGCTTG TGCTGAACTC 
CAGAAACCGG CGCTGACCGG GGCCGATTGG CAGCTCCTGG TAGAGGCCTC AGGCATAACC 
ATCTACCGGC TGCTGGACCA GCCAAGTGGA CTTTATGAGT ATAAGGTGTT CGGTGTCCTG 
GAAGGCTGCT CGCCGGCTCT CCTCGCAGAT GTTTACATGG ACTTAGACTA CAGAAAACAA 
TGGGACCAGT ATGTGAAAGA ACTCTACGAG AAGGAGAGTG ATGAACAGAT GGTGGCATAC 
TGGGAAGTGA AGTACCCTTT CCCGCTGTCC AACAGAGATT ACGTCTACAC CCGCCAGCGC 
CGAGACCTGG ATGTGGACGG GAGGAAGATC TACGTGGTCC TGGCCCAGAG TATCTCGGCA 
CCTCAGTTTC CCGAGAAGTC TGGGGTCATC CGAGTGAAAC AGTACAAACA GAGCCTGGCG 
ATCGAGAGTG ACGGCAAGAA GGGGAGCAGA GAATGGAGTT CCAAACTTTT TGAAGGACAT 
GGTGAAAGCG TGTCAGAACT ACCACAAGAA AACCTAAGGA GGGGACTGGG ACTCCGTCAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303301.1
CDS	53..658
Translation	MAGAACCFSDEQFREACAELQKPALTGADWQLLVEASGITIYRLLDQPSGLYEYKVFGVLEGCSPALLADVYMDLDYRKQWDQYVKELYEKESDEQMVAYWEVKYPFPLSNRDYVYTRQRRDLDVDGRKIYVVLAQSISAPQFPEKSGVIRVKQYKQSLAIESDGKKGSREWSSKLFEGHGESVSELPQENLRRGLGLRHP

Target ORF information:

RefSeq Version	NM_001316372.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylcholine transfer protein (Pctp), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316372.1

ORF Insert Sequence:

ATGGCGGGGG CCGCATGCTG CTTCTCGGAC GAGCAGTTCC GGGAGGCTTG TGCTGAACTC 
CAGAAACCGG CGCTGACCGG GGCCGATTGG CAGCTCCTGG TAGAGGCCTC AGGCATAACC 
ATCTACCGGC TGCTGGACCA GCCAAGTGGA CTTTATGAGT ATAAGGTGTT CGGTGTCCTG 
GAAGGCTGCT CGCCGGCTCT CCTCGCAGAT GTTTACATGG ACTTAGACTA CAGAAAACAA 
TGGGACCAGT ATGTGAAAGA ACTCTACGAG AAGGAGAGTG ATGAACAGAT GGTGGCATAC 
TGGGAAGTGA AGTACCCTTT CCCGCTGTCC AACAGAGATT ACGTCTACAC CCGCCAGCGC 
CGAGACCTGG ATGTGGACGG GAGGAAGATC TACGTGGTCC TGGCCCAGAG TATCTCGGCA 
CCTCAGTTTC CCGAGAAGTC TGGGGTCATC CGAGTGAAAC AGTACAAACA GAGCCTGGCG 
ATCGAGAGTG ACGGCAAGAA GGGGAGCAGA GAATGGAGTT CCAAACTTTT TGAAGGACAT 
GGTGAAAGCG TGTCAGAACT ACCACAAGAA AACCTAAGGA GGGGACTGGG ACTCCGTCAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.