ORF cDNA Clones and Custom Clones » Species Summary » Mus musculus » Pikfyve cDNA ORF clone

Pikfyve cDNA ORF clone, Mus musculus(house mouse)

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Summary

Gene Symbol	Pikfyve
Entrez Gene ID	18711
Full Name	phosphoinositide kinase, FYVE type zinc finger containing
Synonyms	5230400C17Rik,Pip5k,Pip5k3,Pipk5k3,PipkIII,p235
General protein information	Preferred Names phosphoinositide kinase, FYVE type zinc finger containing Names 1-phosphatidylinositol 3-phosphate 5-kinase FYVE finger-containing phosphoinositide kinase PIPkin-III PtdIns(4)P-5-kinase phosphatidylinositol 3-phosphate 5-kinase type III phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III phosphatidylinositol-4-phosphate 5-kinase, type III phosphoinositide kinase, FYVE finger containing type III PIP kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C2

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495782.3	XP_006495845.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006495776.3	XP_006495839.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_030252271.1	XP_030108131.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_006495781.3	XP_006495844.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_011238454.2	XP_011236756.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_006495777.3	XP_006495840.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_006495779.3	XP_006495842.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_006495779.4	XP_006495842.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
NM_011086.2	NP_035216.2	1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
XM_006495777.4	XP_006495840.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_030252267.1	XP_030108127.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_006495782.4	XP_006495845.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_011238454.3	XP_011236756.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_006495781.4	XP_006495844.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
NM_001310624.1	NP_001297553.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform 1

Homologies

Mus musculus (house mouse)	Pikfyve	NP_035216.2
Canis lupus familiaris (dog)	PIKFYVE	XP_005640650.1
Rattus norvegicus (Norway rat)	Pikfyve	XP_006245139.1
Gallus gallus (chicken)	PIKFYVE	XP_004942735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005216	XP_314118.4
Drosophila melanogaster (fruit fly)	fab1	NP_995880.1
Homo sapiens (human)	PIKFYVE	NP_055855.2
Bos taurus (cattle)	PIKFYVE	NP_001192396.1
Pan troglodytes (chimpanzee)	PIKFYVE	XP_516057.2
	PIKFYVE	XP_001108540.1
Danio rerio (zebrafish)	pikfyve	NP_001120777.1

Bibliography

Related articles in PubMed

The Lipid Kinase PIKfyve Coordinates the Neutrophil Immune Response through the Activation of the Rac GTPase.
Dayam RM, Sun CX, Choy CH, Mancuso G, Glogauer M, Botelho RJ
Journal of immunology (Baltimore, Md. : 1950)199(6)2096-2105(2017 Sep)

Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.
Kawasaki T, Ito K, Miyata H, Akira S, Kawai T
The EMBO journal36(12)1707-1718(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that PIKfyve coordinates the neutrophil immune response through the activation of the Rac GTPase
Title: The Lipid Kinase PIKfyve Coordinates the Neutrophil Immune Response through the Activation of the Rac GTPase.

Thus, PIKfyve plays a role in preventing excessive lung inflammation through regulating alveolar macrophage function.
Title: Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.

adipose tissue Pikfyve plays a key role in the mechanisms regulating glucose homeostasis and the PIKfyve pathway is critical in mammary epithelial differentiation during pregnancy and lactogenesis downstream of prolactin receptor signaling.
Title: Unexpected severe consequences of Pikfyve deletion by aP2- or Aq-promoter-driven Cre expression for glucose homeostasis and mammary gland development.

A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity.
Title: A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity.

PIKfyve mediates vesicle motility through the regulation of vesicle integrity in neurons.
Title: PIKfyve mediates the motility of late endosomes and lysosomes in neuronal dendrites.

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The following Pikfyve gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pikfyve cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68434	XM_011238454.3 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu27301	XM_006495777.4 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27302	XM_006495779.4 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27302	XM_030252267.1 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27303	XM_006495781.4 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27304	XM_006495782.4 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85055	XM_030252271.1 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu15175	NM_011086.2 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1511.30 $2159.00
OMu27303	NM_001310624.1 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27301	XM_006495776.3 Latest version!	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu27302	XM_006495779.3	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27301	XM_006495777.3	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27304	XM_006495782.3	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu68434	XM_011238454.2	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu27303	XM_006495781.3	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pikfyve ( XM_011238454.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236756.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu68434
Clone ID Related Accession (Same CDS sequence)	XM_011238454.2 , XM_011238454.3
Accession Version	XM_011238454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGGGCCT GTGTTCTCAC CTCCTACTGC 
TCACAGTCAC TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236756.1
CDS	204..4421
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFRACVLTSYCSQSLC

Target ORF information:

RefSeq Version	XM_011238454.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238454.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGGGCCT GTGTTCTCAC CTCCTACTGC 
TCACAGTCAC TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_011238454.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236756.1 Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X6, mRNA.

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CloneID	OMu68434
Clone ID Related Accession (Same CDS sequence)	XM_011238454.2 , XM_011238454.3
Accession Version	XM_011238454.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGGGCCT GTGTTCTCAC CTCCTACTGC 
TCACAGTCAC TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236756.1
CDS	206..4423
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFRACVLTSYCSQSLC

Target ORF information:

RefSeq Version	XM_011238454.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238454.2

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGGGCCT GTGTTCTCAC CTCCTACTGC 
TCACAGTCAC TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495777.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495840.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu27301
Clone ID Related Accession (Same CDS sequence)	XM_006495776.3 , XM_006495777.3 , XM_006495777.4
Accession Version	XM_006495777.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495777.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAAGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACTA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGCTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGCAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGC GGAACAGTGC TGAAGAAGGG CTCCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGACTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GTAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGAA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCC CAGAAGAGGG AGACCTTGCG AGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCCGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCACAAGA TGCGTGAAGT GATTCTGGGC AGCAGTGAGG AGGAATTCAT CCGTTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495840.1
CDS	149..6478
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495777.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495777.4

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAAGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACTA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGCTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGCAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGC GGAACAGTGC TGAAGAAGGG CTCCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGACTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GTAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGAA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCC CAGAAGAGGG AGACCTTGCG AGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCCGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCACAAGA TGCGTGAAGT GATTCTGGGC AGCAGTGAGG AGGAATTCAT CCGTTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495779.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495842.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

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CloneID	OMu27302
Clone ID Related Accession (Same CDS sequence)	XM_006495779.3 , XM_006495779.4 , XM_030252267.1
Accession Version	XM_006495779.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAGAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAGT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGACTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCATCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACAT TAATGCAAAG CCCTTCATTC 
CATCTTCTGA CGGAGGGACG AGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGAGGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATTCCCTGT 
GCTCTTTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCC 
GTAGGAAATG AACAGCCAGA GACTTCACAG CAAACGGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTTTACAG GATGACACTG GAATGTACGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAGTTAAAA 
GATGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCCTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTAATATGGC CAACAAAGGT GGACTGTCTG 
AACCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGGATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AAGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACTAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGCTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGCAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGCGGA ACAGTGCTGA AGAAGGGCTC CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG ACTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGTA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGAATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCCCAG AAGAGGGAGA CCTTGCGAGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCCGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CACAAGATGC GTGAAGTGAT TCTGGGCAGC AGTGAGGAGG AATTCATCCG TTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495842.1
CDS	204..6530
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495779.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495779.4

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAGAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAGT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGACTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCATCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACAT TAATGCAAAG CCCTTCATTC 
CATCTTCTGA CGGAGGGACG AGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGAGGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATTCCCTGT 
GCTCTTTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCC 
GTAGGAAATG AACAGCCAGA GACTTCACAG CAAACGGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTTTACAG GATGACACTG GAATGTACGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAGTTAAAA 
GATGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCCTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTAATATGGC CAACAAAGGT GGACTGTCTG 
AACCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGGATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AAGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACTAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGCTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGCAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGCGGA ACAGTGCTGA AGAAGGGCTC CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG ACTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGTA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGAATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCCCAG AAGAGGGAGA CCTTGCGAGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCCGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CACAAGATGC GTGAAGTGAT TCTGGGCAGC AGTGAGGAGG AATTCATCCG TTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_030252267.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108127.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

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CloneID	OMu27302
Clone ID Related Accession (Same CDS sequence)	XM_006495779.3 , XM_006495779.4 , XM_030252267.1
Accession Version	XM_030252267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAGAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAGT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGACTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCATCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACAT TAATGCAAAG CCCTTCATTC 
CATCTTCTGA CGGAGGGACG AGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGAGGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATTCCCTGT 
GCTCTTTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCC 
GTAGGAAATG AACAGCCAGA GACTTCACAG CAAACGGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTTTACAG GATGACACTG GAATGTACGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAGTTAAAA 
GATGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCCTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTAATATGGC CAACAAAGGT GGACTGTCTG 
AACCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGGATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AAGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACTAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGCTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGCAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGCGGA ACAGTGCTGA AGAAGGGCTC CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG ACTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGTA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGAATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCCCAG AAGAGGGAGA CCTTGCGAGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCCGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CACAAGATGC GTGAAGTGAT TCTGGGCAGC AGTGAGGAGG AATTCATCCG TTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108127.1
CDS	149..6475
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_030252267.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252267.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAGAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAGT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGACTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCATCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACAT TAATGCAAAG CCCTTCATTC 
CATCTTCTGA CGGAGGGACG AGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGAGGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATTCCCTGT 
GCTCTTTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCC 
GTAGGAAATG AACAGCCAGA GACTTCACAG CAAACGGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTTTACAG GATGACACTG GAATGTACGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAGTTAAAA 
GATGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCCTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTAATATGGC CAACAAAGGT GGACTGTCTG 
AACCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGGATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AAGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACTAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGCTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGCAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGCGGA ACAGTGCTGA AGAAGGGCTC CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG ACTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGTA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGAATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCCCAG AAGAGGGAGA CCTTGCGAGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCCGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CACAAGATGC GTGAAGTGAT TCTGGGCAGC AGTGAGGAGG AATTCATCCG TTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495781.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495844.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

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CloneID	OMu27303
Clone ID Related Accession (Same CDS sequence)	XM_006495781.3 , XM_006495781.4 , NM_001310624.1
Accession Version	XM_006495781.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495781.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AGAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAGTTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA CTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ATCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACATTAA TGCAAAGCCC TTCATTCCAT CTTCTGACGG AGGGACGAGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGAGGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT TCCCTGTGCT CTTTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCCGTA GGAAATGAAC AGCCAGAGAC TTCACAGCAA 
ACGGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TTTACAGGAT 
GACACTGGAA TGTACGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA GTTAAAAGAT GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAGCTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCCTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTA 
ATATGGCCAA CAAAGGTGGA CTGTCTGAAC CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GGATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAAG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACTAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGC 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGCGGAACA GTGCTGAAGA AGGGCTCCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGACT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGTAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGAATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCCCAGAAG 
AGGGAGACCT TGCGAGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC CGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ATGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAC AAGATGCGTG AAGTGATTCT GGGCAGCAGT 
GAGGAGGAAT TCATCCGTTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGTTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495844.1
CDS	149..6442
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495781.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495781.4

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AGAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAGTTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA CTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ATCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACATTAA TGCAAAGCCC TTCATTCCAT CTTCTGACGG AGGGACGAGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGAGGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT TCCCTGTGCT CTTTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCCGTA GGAAATGAAC AGCCAGAGAC TTCACAGCAA 
ACGGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TTTACAGGAT 
GACACTGGAA TGTACGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA GTTAAAAGAT GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAGCTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCCTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTA 
ATATGGCCAA CAAAGGTGGA CTGTCTGAAC CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GGATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAAG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACTAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGC 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGCGGAACA GTGCTGAAGA AGGGCTCCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGACT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGTAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGAATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCCCAGAAG 
AGGGAGACCT TGCGAGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC CGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ATGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAC AAGATGCGTG AAGTGATTCT GGGCAGCAGT 
GAGGAGGAAT TCATCCGTTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGTTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495782.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495845.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

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CloneID	OMu27304
Clone ID Related Accession (Same CDS sequence)	XM_006495782.3 , XM_006495782.4
Accession Version	XM_006495782.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495782.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGAGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCCTACTCCA GCAGTTGCTT 
CAAAACGAGT CATTGTCATC GTCTTGGAGG GACATCATTG TGTCACTAGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGACT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACCTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCTCA GGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACTTG 
GGCACTTGCC ACAAATTTTA TATGCAGATA TTTCAGCTGC CTAATGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAT CTAGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC TTTTCTCATG GATGAGTTCG CTATGCCTCC AACATTAATG 
CAAAGCCCTT CATTCCATCT TCTGACGGAG GGACGAGGTG AAGAGGGAGC CTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCTCAGGAT CCTGAGTGCC CTCGTGAGGC CCTGTCTTCT 
GAGGATAGCA CTTTGTTGGA ATCAAGGACT GTGCTAGAGA AGGGTGAACT AGACAATAAA 
AGTATTCCAC AAGCTGTTGC CTCTTTGAAG CATCAAGATT ATACCACCCC CACTTGCCCA 
GCAGGTATTC CCTGTGCTCT TTTTGCATTG GTACCAGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCCGTAGG AAATGAACAG CCAGAGACTT CACAGCAAAC GGATGAGCAA 
CAGGATCCCA AAAGCCAGAT GAAAGCTTTT AGAGACCCTT TACAGGATGA CACTGGAATG 
TACGTTACTG AGGAAGTCAC CTCCTCTGAA GATCAACGAA AGACTTATGC CTTGACATTT 
AAACAGGAGT TAAAAGATGT AATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGATGC TCAACTCGAG ATTATTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA CAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGCA GTGTTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACCAGGATTG CTGAACATCT GGGTGACAGC 
CAGACCTTGG GTAGAATGCT AGCTGATTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CCTTTGTCCA TTCAAAAGAT GCATCATGTA CTTCAGGTGG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTAAT ATGGCCAACA 
AAGGTGGACT GTCTGAACCC TGCTAACCAC CAGAGGCTCT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCT TGTGTCAGTC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGG ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCGAGCAT GTTCTGTGAC ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATT CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCTATG TCATTTGCAA AGTACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACACG CAGAGCCAAC GCTGAGCCCT GCGGTCACTC TATCCACCAT 
GATTATCACC AGTATTTCTC TTATAACCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTATGTGT TCCACTACCA AAAATATTCA TTAAGCGTCA AGCCCCACTG 
AAGGTATCTC TTCTTCAGGA CCTCAAAGAC TTTTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AGAGACTTGC ATCCTTGAAA ACGGATACAT TTAGCAAAAC TAGAGAGGAA 
AAGATGGAAG ATATCTTTGC ACAAAAGGAG ATGGAAGAGG GTGAGTTTAA GAACTGGACA 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTGGATA CCCCTCAGCA ACTGCAGTCC 
ATTTTTGAGT CACTGATTGC CAAGAAGCAA AGCCTCTGTG AGGTGCTCCA GGCGTGGAAC 
AGCAGGTTGC AGGACCTCTT CCAGCAGGAA AAAGGTAGAA AGAGGCCTTC AGTTCCTCCC 
AGTCCTGGGA GACTGAGACA AGGTGAAGAA AGCAAGATAA ATGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAATGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGCCAGA GCTCTACGAG CTCCACCCAC CTCCAGCTGC CCACTCCTCC CGAGGCCCTG 
GCCGAGCAGG TAGTGGGAGG GCCGACTGAT CTGGATTCAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGTC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTACAATC CCATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGCAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GCGGAACAGT GCTGAAGAAG GGCTCCCAGC CAATAGTGCT 
TTAGATAACA GACCTAAGAG TAGCAGCCCT ATTAGACTAC CTGAAATCAG TGGAGGACAG 
ACAAACCGCA CAGTAGAAGC AGAACCTCAG CCAACCAAAA AAGCTTCAGG AATGTTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CCCAGAAGAG GGAGACCTTG 
CGAGGGGCAG ACAGTGCTTA CTACCAGGTT GGGCAGGCCG GCAAGGAGGG GTTGGAGAGT 
CAAGGCCTGG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTCACA 
AATCCTCATG TGGAACTTCA ATTTTCTGAT GCTAATGCCA AGTTTTACTG TCGGCTGTAC 
TACGCGGGAG AGTTCCACAA GATGCGTGAA GTGATTCTGG GCAGCAGTGA GGAGGAATTC 
ATCCGTTCCC TTTCTCACTC ATCTCCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CCGAAGATGA TAGATTCATT CTGAAGCAAA TGCCTCGTTT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC ACACTACTTC AATTATATCA CAAATGCTGT TCAACAAAAG 
AGGCCCACCG CCTTGGCTAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACTGACACT 
GGGAAAGAGA GCTGTGATGT GGTTCTGTTG GATGAAAACC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTCT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCACAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTC TGCTGGTTGG GCGAGATGAC 
ACTAGCAATG AGCTTGTGGT TGGCATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTTGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACT 
GTGGTCTCTC CAGAGTTGTA TAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495845.1
CDS	204..6365
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495782.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495782.4

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGAGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCCTACTCCA GCAGTTGCTT 
CAAAACGAGT CATTGTCATC GTCTTGGAGG GACATCATTG TGTCACTAGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGACT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACCTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCTCA GGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACTTG 
GGCACTTGCC ACAAATTTTA TATGCAGATA TTTCAGCTGC CTAATGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAT CTAGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC TTTTCTCATG GATGAGTTCG CTATGCCTCC AACATTAATG 
CAAAGCCCTT CATTCCATCT TCTGACGGAG GGACGAGGTG AAGAGGGAGC CTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCTCAGGAT CCTGAGTGCC CTCGTGAGGC CCTGTCTTCT 
GAGGATAGCA CTTTGTTGGA ATCAAGGACT GTGCTAGAGA AGGGTGAACT AGACAATAAA 
AGTATTCCAC AAGCTGTTGC CTCTTTGAAG CATCAAGATT ATACCACCCC CACTTGCCCA 
GCAGGTATTC CCTGTGCTCT TTTTGCATTG GTACCAGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCCGTAGG AAATGAACAG CCAGAGACTT CACAGCAAAC GGATGAGCAA 
CAGGATCCCA AAAGCCAGAT GAAAGCTTTT AGAGACCCTT TACAGGATGA CACTGGAATG 
TACGTTACTG AGGAAGTCAC CTCCTCTGAA GATCAACGAA AGACTTATGC CTTGACATTT 
AAACAGGAGT TAAAAGATGT AATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGATGC TCAACTCGAG ATTATTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA CAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGCA GTGTTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACCAGGATTG CTGAACATCT GGGTGACAGC 
CAGACCTTGG GTAGAATGCT AGCTGATTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CCTTTGTCCA TTCAAAAGAT GCATCATGTA CTTCAGGTGG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTAAT ATGGCCAACA 
AAGGTGGACT GTCTGAACCC TGCTAACCAC CAGAGGCTCT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCT TGTGTCAGTC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGG ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCGAGCAT GTTCTGTGAC ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATT CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCTATG TCATTTGCAA AGTACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACACG CAGAGCCAAC GCTGAGCCCT GCGGTCACTC TATCCACCAT 
GATTATCACC AGTATTTCTC TTATAACCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTATGTGT TCCACTACCA AAAATATTCA TTAAGCGTCA AGCCCCACTG 
AAGGTATCTC TTCTTCAGGA CCTCAAAGAC TTTTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AGAGACTTGC ATCCTTGAAA ACGGATACAT TTAGCAAAAC TAGAGAGGAA 
AAGATGGAAG ATATCTTTGC ACAAAAGGAG ATGGAAGAGG GTGAGTTTAA GAACTGGACA 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTGGATA CCCCTCAGCA ACTGCAGTCC 
ATTTTTGAGT CACTGATTGC CAAGAAGCAA AGCCTCTGTG AGGTGCTCCA GGCGTGGAAC 
AGCAGGTTGC AGGACCTCTT CCAGCAGGAA AAAGGTAGAA AGAGGCCTTC AGTTCCTCCC 
AGTCCTGGGA GACTGAGACA AGGTGAAGAA AGCAAGATAA ATGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAATGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGCCAGA GCTCTACGAG CTCCACCCAC CTCCAGCTGC CCACTCCTCC CGAGGCCCTG 
GCCGAGCAGG TAGTGGGAGG GCCGACTGAT CTGGATTCAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGTC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTACAATC CCATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGCAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GCGGAACAGT GCTGAAGAAG GGCTCCCAGC CAATAGTGCT 
TTAGATAACA GACCTAAGAG TAGCAGCCCT ATTAGACTAC CTGAAATCAG TGGAGGACAG 
ACAAACCGCA CAGTAGAAGC AGAACCTCAG CCAACCAAAA AAGCTTCAGG AATGTTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CCCAGAAGAG GGAGACCTTG 
CGAGGGGCAG ACAGTGCTTA CTACCAGGTT GGGCAGGCCG GCAAGGAGGG GTTGGAGAGT 
CAAGGCCTGG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTCACA 
AATCCTCATG TGGAACTTCA ATTTTCTGAT GCTAATGCCA AGTTTTACTG TCGGCTGTAC 
TACGCGGGAG AGTTCCACAA GATGCGTGAA GTGATTCTGG GCAGCAGTGA GGAGGAATTC 
ATCCGTTCCC TTTCTCACTC ATCTCCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CCGAAGATGA TAGATTCATT CTGAAGCAAA TGCCTCGTTT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC ACACTACTTC AATTATATCA CAAATGCTGT TCAACAAAAG 
AGGCCCACCG CCTTGGCTAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACTGACACT 
GGGAAAGAGA GCTGTGATGT GGTTCTGTTG GATGAAAACC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTCT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCACAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTC TGCTGGTTGG GCGAGATGAC 
ACTAGCAATG AGCTTGTGGT TGGCATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTTGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACT 
GTGGTCTCTC CAGAGTTGTA TAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_030252271.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108131.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

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CloneID	OMu85055
Clone ID Related Accession (Same CDS sequence)	XM_030252271.1
Accession Version	XM_030252271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAG TATTTGGTTT CTGACACTGG AGGACAGCAG 
CTCTCAATAA GTGATGCCTT CATCAAAGAG TCCTTATTTA ATCGACGAGT AGAGGAAAAA 
TCCAAAGAGC TGCCTTTTAC CCCTTTGGGC TGGCATCATA ACAACCTGGA ACTCTTGCGA 
GAGGAGAATG AGGAGAAGCA AGCCATGGAA AGGCTGCTTT CAGCTAATCA TAACCACATG 
ATGGCCCTAC TCCAGCAGTT GCTTCAAAAC GAGTCATTGT CATCGTCTTG GAGGGACATC 
ATTGTGTCAC TAGTCTGCCA GGTTGTTCAG ACAGTCCGAC CTGATGTCAA GCACCAGGAT 
GATGACATGG ATATCCGTCA GTTTGTCCAT ATCAAGAAGA TCCCAGGTGG AAAGAAATTT 
GACTCTGTGG TTGTCAATGG CTTTGTTTGT ACCAAGAACA TTGCACATAA AAAGATGAAT 
TCCTGTATTA AAAACCCCAA AATCCTTCTG TTGAAGTGTT CTATTGAGTA TCTCTATAGA 
GAAGAAACTA AGTTTACCTG CATTGATCCT ATTGTGCTTC AGGAAAGGGA ATTCTTGAAG 
AATTATGTTC AACGAATAGT TGATGTTCGA CCCACATTGG TTCTTGTTGA GAAAACAGTG 
TCTCGGATTG CTCAGGACAT GTTACTGGAA CATGGCATTA CTCTGGTCAT TAATGTAAAG 
TCACAAGTTT TAGAAAGAAT CAGTCGAATG ACCCAAGGTG ATTTAGTGGT GTCCATGGAC 
CAGCTGCTCA CCAAACCCCA CTTGGGCACT TGCCACAAAT TTTATATGCA GATATTTCAG 
CTGCCTAATG AACAAACCAA AACACTGATG TTTTTTGAAG GCTGTCCACA GCATCTAGGC 
TGCACAATCA AGCTCAGAGG AGGCTCTGAT TATGAGCTGG CTCGAGTTAA GGAGATCCTA 
ATATTTATGA TCTGTGTAGC TTATCATTCT CAGCTAGAAA TCTCTTTTCT CATGGATGAG 
TTCGCTATGC CTCCAACATT AATGCAAAGC CCTTCATTCC ATCTTCTGAC GGAGGGACGA 
GGTGAAGAGG GAGCCTCTCA GGAGCAGGTC AGTGGCAGCT CCCTTCCTCA GGATCCTGAG 
TGCCCTCGTG AGGCCCTGTC TTCTGAGGAT AGCACTTTGT TGGAATCAAG GACTGTGCTA 
GAGAAGGGTG AACTAGACAA TAAAAGTATT CCACAAGCTG TTGCCTCTTT GAAGCATCAA 
GATTATACCA CCCCCACTTG CCCAGCAGGT ATTCCCTGTG CTCTTTTTGC ATTGGTACCA 
GAGTCATTGT TGCCTCTCCA TATGGATCAA CAGGATGCCG TAGGAAATGA ACAGCCAGAG 
ACTTCACAGC AAACGGATGA GCAACAGGAT CCCAAAAGCC AGATGAAAGC TTTTAGAGAC 
CCTTTACAGG ATGACACTGG AATGTACGTT ACTGAGGAAG TCACCTCCTC TGAAGATCAA 
CGAAAGACTT ATGCCTTGAC ATTTAAACAG GAGTTAAAAG ATGTAATCCT CTGTATCTCT 
CCAGTTATTA CATTCCGTGA ACCTTTCCTT TTAACTGAAA AGGGGATGAG ATGCTCAACT 
CGAGATTATT TTCCAGAGCA GATTTACTGG TCTCCTCTTC TCAACAAAGA GGTGAAGGAA 
ATGGAGAGCA GGAGGAAGAA ACAGCTGCTC AGGGATCTCT CTGGACTTCA GGGCATGAAT 
GGCAGTGTTC AGGCCAAGTC TATTCAAGTC TTACCCTCAC ATGAGCTAGT GAGCACCAGG 
ATTGCTGAAC ATCTGGGTGA CAGCCAGACC TTGGGTAGAA TGCTAGCTGA TTATCGAGCT 
AGAGGAGGAA GAATTCAGTC AAAACATTTG GACCCCTTTG TCCATTCAAA AGATGCATCA 
TGTACTTCAG GTGGCAAATC AGGAAACAAA ACTGAGAGTG ATGAAGAGAG GGGATTGATT 
CCAAGTGATG TAATATGGCC AACAAAGGTG GACTGTCTGA ACCCTGCTAA CCACCAGAGG 
CTCTGTGTGC TCTTCAGCAG CTCTTCTGCC CAGTCCAGCA ATGCTCCCAG TGCTTGTGTC 
AGTCCTTGGA TTGTAACAAT GGAGTTTTAT GGAAAGAATG ACCTTACACT GGGGATATTT 
TTAGAAAGAT ACTGTTTCAG GTCTTCTTAC CAGTGTCCGA GCATGTTCTG TGACACCCCC 
ATGGTTCATC ACATTCGACG CTTTGTTCAT GGCCAAGGCT GTGTACAGAT AATTCTGAAG 
GAGTTGGATT CTCCAGTGCC TGGATATCAA CATACAATTC TCACATATTC CTGGTGCAGA 
ATCTGCAAAC AAGTAACACC AGTTGTTGCT CTTTCAAATG AATCCTGGTC TATGTCATTT 
GCAAAGTACC TTGAACTTCG ATTTTATGGC CACCAGTACA CACGCAGAGC CAACGCTGAG 
CCCTGCGGTC ACTCTATCCA CCATGATTAT CACCAGTATT TCTCTTATAA CCAGATGGTG 
GCATCTTTCA GTTACTCTCC TATTCGGCTT CTTGAAGTAT GTGTTCCACT ACCAAAAATA 
TTCATTAAGC GTCAAGCCCC ACTGAAGGTA TCTCTTCTTC AGGACCTCAA AGACTTTTTT 
CAGAAGGTTT CACAGGTGTA CCTAGCTGTT GATGAGAGAC TTGCATCCTT GAAAACGGAT 
ACATTTAGCA AAACTAGAGA GGAAAAGATG GAAGATATCT TTGCACAAAA GGAGATGGAA 
GAGGGTGAGT TTAAGAACTG GACAGAGAAG ATGCAAGCAA GGCTCATGTC TTCCTCTGTG 
GATACCCCTC AGCAACTGCA GTCCATTTTT GAGTCACTGA TTGCCAAGAA GCAAAGCCTC 
TGTGAGGTGC TCCAGGCGTG GAACAGCAGG TTGCAGGACC TCTTCCAGCA GGAAAAAGGT 
AGAAAGAGGC CTTCAGTTCC TCCCAGTCCT GGGAGACTGA GACAAGGTGA AGAAAGCAAG 
ATAAATGCAA TGGACACATC TCCAAGGAAT ATTTCTCCAG GACTTCACAA TGGAGAAAAA 
GAGGATCGCT TCTTGACAAC CCTGTCCAGC CAGAGCTCTA CGAGCTCCAC CCACCTCCAG 
CTGCCCACTC CTCCCGAGGC CCTGGCCGAG CAGGTAGTGG GAGGGCCGAC TGATCTGGAT 
TCAGCCAGTG GCTCTGAAGA TGTATTTGAT GGTCATTTGC TGGGATCCAC AGACAGCCAG 
GTGAAGGAAA AGTCAACCAT GAAAGCCATC TTTGCTAATT TGCTTCCAGG AAACAGCTAC 
AATCCCATTC CATTTCCTTT TGATCCAGAT AAACACTACT TAATGTATGA ACATGAACGG 
GTGCCCATTG CTGTCTGTGA GAAAGAGCCC AGCTCCATCA TTGCTTTTGC ACTCAGTTGT 
AAAGAATACC GCAATGCCTT AGAGGAATTG TCCAAAGCAA CTCTGCGGAA CAGTGCTGAA 
GAAGGGCTCC CAGCCAATAG TGCTTTAGAT AACAGACCTA AGAGTAGCAG CCCTATTAGA 
CTACCTGAAA TCAGTGGAGG ACAGACAAAC CGCACAGTAG AAGCAGAACC TCAGCCAACC 
AAAAAAGCTT CAGGAATGTT GTCCTTCTTC AGAGGAACAG CAGGGAAGAG CCCTGATCTG 
TCTTCCCAGA AGAGGGAGAC CTTGCGAGGG GCAGACAGTG CTTACTACCA GGTTGGGCAG 
GCCGGCAAGG AGGGGTTGGA GAGTCAAGGC CTGGAGCCTC AAGATGAAGT AGATGGAGGA 
GATACACAGA AGAAACAACT CACAAATCCT CATGTGGAAC TTCAATTTTC TGATGCTAAT 
GCCAAGTTTT ACTGTCGGCT GTACTACGCG GGAGAGTTCC ACAAGATGCG TGAAGTGATT 
CTGGGCAGCA GTGAGGAGGA ATTCATCCGT TCCCTTTCTC ACTCATCTCC CTGGCAGGCC 
CGGGGAGGCA AGTCAGGAGC TGCTTTCTAT GCCACCGAAG ATGATAGATT CATTCTGAAG 
CAAATGCCTC GTTTGGAAGT CCAGTCTTTC CTTGACTTTG CACCACACTA CTTCAATTAT 
ATCACAAATG CTGTTCAACA AAAGAGGCCC ACCGCCTTGG CTAAAATTCT TGGAGTTTAC 
AGAATTGGTT ATAAGAACTC TCAGAACAAC ACTGAGAAGA AGTTAGATCT CCTTGTCATG 
GAAAATCTTT TCTATGGGAG AAAGATGGCA CAGGTTTTTG ATTTGAAGGG TTCACTTAGG 
AATCGAAATG TAAAAACTGA CACTGGGAAA GAGAGCTGTG ATGTGGTTCT GTTGGATGAA 
AACCTCCTAA AGATGGTTCG AGACAACCCT CTCTATATTC GTTCCCATTC CAAATCTGTG 
CTGAGAACCT CCATCCACAG CGACGCCCAT TTCCTTTCCA GCCACCTCAT TATAGACTAT 
TCTCTGCTGG TTGGGCGAGA TGACACTAGC AATGAGCTTG TGGTTGGCAT CATAGATTAC 
ATTCGAACAT TTACATGGGA CAAAAAACTT GAGATGGTTG TGAAGTCAAC AGGAATTTTA 
GGAGGACAAG GTAAAATGCC AACTGTGGTC TCTCCAGAGT TGTATAGGAC TAGATTTTGT 
GAAGCAATGG ACAAGTATTT CTTGATGGTG CCAGACCACT GGACAGGGTT GGATCTGAAT 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108131.1
CDS	204..6329
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_030252271.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252271.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAG TATTTGGTTT CTGACACTGG AGGACAGCAG 
CTCTCAATAA GTGATGCCTT CATCAAAGAG TCCTTATTTA ATCGACGAGT AGAGGAAAAA 
TCCAAAGAGC TGCCTTTTAC CCCTTTGGGC TGGCATCATA ACAACCTGGA ACTCTTGCGA 
GAGGAGAATG AGGAGAAGCA AGCCATGGAA AGGCTGCTTT CAGCTAATCA TAACCACATG 
ATGGCCCTAC TCCAGCAGTT GCTTCAAAAC GAGTCATTGT CATCGTCTTG GAGGGACATC 
ATTGTGTCAC TAGTCTGCCA GGTTGTTCAG ACAGTCCGAC CTGATGTCAA GCACCAGGAT 
GATGACATGG ATATCCGTCA GTTTGTCCAT ATCAAGAAGA TCCCAGGTGG AAAGAAATTT 
GACTCTGTGG TTGTCAATGG CTTTGTTTGT ACCAAGAACA TTGCACATAA AAAGATGAAT 
TCCTGTATTA AAAACCCCAA AATCCTTCTG TTGAAGTGTT CTATTGAGTA TCTCTATAGA 
GAAGAAACTA AGTTTACCTG CATTGATCCT ATTGTGCTTC AGGAAAGGGA ATTCTTGAAG 
AATTATGTTC AACGAATAGT TGATGTTCGA CCCACATTGG TTCTTGTTGA GAAAACAGTG 
TCTCGGATTG CTCAGGACAT GTTACTGGAA CATGGCATTA CTCTGGTCAT TAATGTAAAG 
TCACAAGTTT TAGAAAGAAT CAGTCGAATG ACCCAAGGTG ATTTAGTGGT GTCCATGGAC 
CAGCTGCTCA CCAAACCCCA CTTGGGCACT TGCCACAAAT TTTATATGCA GATATTTCAG 
CTGCCTAATG AACAAACCAA AACACTGATG TTTTTTGAAG GCTGTCCACA GCATCTAGGC 
TGCACAATCA AGCTCAGAGG AGGCTCTGAT TATGAGCTGG CTCGAGTTAA GGAGATCCTA 
ATATTTATGA TCTGTGTAGC TTATCATTCT CAGCTAGAAA TCTCTTTTCT CATGGATGAG 
TTCGCTATGC CTCCAACATT AATGCAAAGC CCTTCATTCC ATCTTCTGAC GGAGGGACGA 
GGTGAAGAGG GAGCCTCTCA GGAGCAGGTC AGTGGCAGCT CCCTTCCTCA GGATCCTGAG 
TGCCCTCGTG AGGCCCTGTC TTCTGAGGAT AGCACTTTGT TGGAATCAAG GACTGTGCTA 
GAGAAGGGTG AACTAGACAA TAAAAGTATT CCACAAGCTG TTGCCTCTTT GAAGCATCAA 
GATTATACCA CCCCCACTTG CCCAGCAGGT ATTCCCTGTG CTCTTTTTGC ATTGGTACCA 
GAGTCATTGT TGCCTCTCCA TATGGATCAA CAGGATGCCG TAGGAAATGA ACAGCCAGAG 
ACTTCACAGC AAACGGATGA GCAACAGGAT CCCAAAAGCC AGATGAAAGC TTTTAGAGAC 
CCTTTACAGG ATGACACTGG AATGTACGTT ACTGAGGAAG TCACCTCCTC TGAAGATCAA 
CGAAAGACTT ATGCCTTGAC ATTTAAACAG GAGTTAAAAG ATGTAATCCT CTGTATCTCT 
CCAGTTATTA CATTCCGTGA ACCTTTCCTT TTAACTGAAA AGGGGATGAG ATGCTCAACT 
CGAGATTATT TTCCAGAGCA GATTTACTGG TCTCCTCTTC TCAACAAAGA GGTGAAGGAA 
ATGGAGAGCA GGAGGAAGAA ACAGCTGCTC AGGGATCTCT CTGGACTTCA GGGCATGAAT 
GGCAGTGTTC AGGCCAAGTC TATTCAAGTC TTACCCTCAC ATGAGCTAGT GAGCACCAGG 
ATTGCTGAAC ATCTGGGTGA CAGCCAGACC TTGGGTAGAA TGCTAGCTGA TTATCGAGCT 
AGAGGAGGAA GAATTCAGTC AAAACATTTG GACCCCTTTG TCCATTCAAA AGATGCATCA 
TGTACTTCAG GTGGCAAATC AGGAAACAAA ACTGAGAGTG ATGAAGAGAG GGGATTGATT 
CCAAGTGATG TAATATGGCC AACAAAGGTG GACTGTCTGA ACCCTGCTAA CCACCAGAGG 
CTCTGTGTGC TCTTCAGCAG CTCTTCTGCC CAGTCCAGCA ATGCTCCCAG TGCTTGTGTC 
AGTCCTTGGA TTGTAACAAT GGAGTTTTAT GGAAAGAATG ACCTTACACT GGGGATATTT 
TTAGAAAGAT ACTGTTTCAG GTCTTCTTAC CAGTGTCCGA GCATGTTCTG TGACACCCCC 
ATGGTTCATC ACATTCGACG CTTTGTTCAT GGCCAAGGCT GTGTACAGAT AATTCTGAAG 
GAGTTGGATT CTCCAGTGCC TGGATATCAA CATACAATTC TCACATATTC CTGGTGCAGA 
ATCTGCAAAC AAGTAACACC AGTTGTTGCT CTTTCAAATG AATCCTGGTC TATGTCATTT 
GCAAAGTACC TTGAACTTCG ATTTTATGGC CACCAGTACA CACGCAGAGC CAACGCTGAG 
CCCTGCGGTC ACTCTATCCA CCATGATTAT CACCAGTATT TCTCTTATAA CCAGATGGTG 
GCATCTTTCA GTTACTCTCC TATTCGGCTT CTTGAAGTAT GTGTTCCACT ACCAAAAATA 
TTCATTAAGC GTCAAGCCCC ACTGAAGGTA TCTCTTCTTC AGGACCTCAA AGACTTTTTT 
CAGAAGGTTT CACAGGTGTA CCTAGCTGTT GATGAGAGAC TTGCATCCTT GAAAACGGAT 
ACATTTAGCA AAACTAGAGA GGAAAAGATG GAAGATATCT TTGCACAAAA GGAGATGGAA 
GAGGGTGAGT TTAAGAACTG GACAGAGAAG ATGCAAGCAA GGCTCATGTC TTCCTCTGTG 
GATACCCCTC AGCAACTGCA GTCCATTTTT GAGTCACTGA TTGCCAAGAA GCAAAGCCTC 
TGTGAGGTGC TCCAGGCGTG GAACAGCAGG TTGCAGGACC TCTTCCAGCA GGAAAAAGGT 
AGAAAGAGGC CTTCAGTTCC TCCCAGTCCT GGGAGACTGA GACAAGGTGA AGAAAGCAAG 
ATAAATGCAA TGGACACATC TCCAAGGAAT ATTTCTCCAG GACTTCACAA TGGAGAAAAA 
GAGGATCGCT TCTTGACAAC CCTGTCCAGC CAGAGCTCTA CGAGCTCCAC CCACCTCCAG 
CTGCCCACTC CTCCCGAGGC CCTGGCCGAG CAGGTAGTGG GAGGGCCGAC TGATCTGGAT 
TCAGCCAGTG GCTCTGAAGA TGTATTTGAT GGTCATTTGC TGGGATCCAC AGACAGCCAG 
GTGAAGGAAA AGTCAACCAT GAAAGCCATC TTTGCTAATT TGCTTCCAGG AAACAGCTAC 
AATCCCATTC CATTTCCTTT TGATCCAGAT AAACACTACT TAATGTATGA ACATGAACGG 
GTGCCCATTG CTGTCTGTGA GAAAGAGCCC AGCTCCATCA TTGCTTTTGC ACTCAGTTGT 
AAAGAATACC GCAATGCCTT AGAGGAATTG TCCAAAGCAA CTCTGCGGAA CAGTGCTGAA 
GAAGGGCTCC CAGCCAATAG TGCTTTAGAT AACAGACCTA AGAGTAGCAG CCCTATTAGA 
CTACCTGAAA TCAGTGGAGG ACAGACAAAC CGCACAGTAG AAGCAGAACC TCAGCCAACC 
AAAAAAGCTT CAGGAATGTT GTCCTTCTTC AGAGGAACAG CAGGGAAGAG CCCTGATCTG 
TCTTCCCAGA AGAGGGAGAC CTTGCGAGGG GCAGACAGTG CTTACTACCA GGTTGGGCAG 
GCCGGCAAGG AGGGGTTGGA GAGTCAAGGC CTGGAGCCTC AAGATGAAGT AGATGGAGGA 
GATACACAGA AGAAACAACT CACAAATCCT CATGTGGAAC TTCAATTTTC TGATGCTAAT 
GCCAAGTTTT ACTGTCGGCT GTACTACGCG GGAGAGTTCC ACAAGATGCG TGAAGTGATT 
CTGGGCAGCA GTGAGGAGGA ATTCATCCGT TCCCTTTCTC ACTCATCTCC CTGGCAGGCC 
CGGGGAGGCA AGTCAGGAGC TGCTTTCTAT GCCACCGAAG ATGATAGATT CATTCTGAAG 
CAAATGCCTC GTTTGGAAGT CCAGTCTTTC CTTGACTTTG CACCACACTA CTTCAATTAT 
ATCACAAATG CTGTTCAACA AAAGAGGCCC ACCGCCTTGG CTAAAATTCT TGGAGTTTAC 
AGAATTGGTT ATAAGAACTC TCAGAACAAC ACTGAGAAGA AGTTAGATCT CCTTGTCATG 
GAAAATCTTT TCTATGGGAG AAAGATGGCA CAGGTTTTTG ATTTGAAGGG TTCACTTAGG 
AATCGAAATG TAAAAACTGA CACTGGGAAA GAGAGCTGTG ATGTGGTTCT GTTGGATGAA 
AACCTCCTAA AGATGGTTCG AGACAACCCT CTCTATATTC GTTCCCATTC CAAATCTGTG 
CTGAGAACCT CCATCCACAG CGACGCCCAT TTCCTTTCCA GCCACCTCAT TATAGACTAT 
TCTCTGCTGG TTGGGCGAGA TGACACTAGC AATGAGCTTG TGGTTGGCAT CATAGATTAC 
ATTCGAACAT TTACATGGGA CAAAAAACTT GAGATGGTTG TGAAGTCAAC AGGAATTTTA 
GGAGGACAAG GTAAAATGCC AACTGTGGTC TCTCCAGAGT TGTATAGGAC TAGATTTTGT 
GAAGCAATGG ACAAGTATTT CTTGATGGTG CCAGACCACT GGACAGGGTT GGATCTGAAT 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( NM_011086.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035216.2 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant 2, mRNA.

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CloneID	OMu15175
Clone ID Related Accession (Same CDS sequence)	NM_011086.2
Accession Version	NM_011086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY271210.1, AF102777.1, AK173077.1 and AC164079.11. On Oct 5, 2006 this sequence version replaced NM_011086.1. Transcript Variant: This variant (2) contains an alternate in-frame exon and lacks another alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF102777.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGAGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCC TACTCCAGCA GTTGCTTCAA 
AACGAGTCAT TGTCATCGTC TTGGAGGGAC ATCATTGTGT CACTAGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGACTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC CTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCTCAGGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACTTGGGC 
ACTTGCCACA AATTTTATAT GCAGATATTT CAGCTGCCTA ATGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTCC ACAGCATCTA GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCTTT TCTCATGGAT GAGTTCGCTA TGCCTCCAAC ATTAATGCAA 
AGCCCTTCAT TCCATCTTCT GACGGAGGGA CGAGGTGAAG AGGGAGCCTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC TCAGGATCCT GAGTGCCCTC GTGAGGCCCT GTCTTCTGAG 
GATAGCACTT TGTTGGAATC AAGGACTGTG CTAGAGAAGG GTGAACTAGA CAATAAAAGT 
ATTCCACAAG CTGTTGCCTC TTTGAAGCAT CAAGATTATA CCACCCCCAC TTGCCCAGCA 
GGTATTCCCT GTGCTCTTTT TGCATTGGTA CCAGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CCGTAGGAAA TGAACAGCCA GAGACTTCAC AGCAAACGGA TGAGCAACAG 
GATCCCAAAA GCCAGATGAA AGCTTTTAGA GACCCTTTAC AGGATGACAC TGGAATGTAC 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT CAACGAAAGA CTTATGCCTT GACATTTAAA 
CAGGAGTTAA AAGATGTAAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGATGCTCA ACTCGAGATT ATTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAACAA AGAGGTGAAG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGCAGTG TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAGCT AGTGAGCACC AGGATTGCTG AACATCTGGG TGACAGCCAG 
ACCTTGGGTA GAATGCTAGC TGATTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCCT TTGTCCATTC AAAAGATGCA TCATGTACTT CAGGTGGCAA ATCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATTCCAAGTG ATGTAATATG GCCAACAAAG 
GTGGACTGTC TGAACCCTGC TAACCACCAG AGGCTCTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC CAGTGCTTGT GTCAGTCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGGATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CGAGCATGTT CTGTGACACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATTCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA TTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCTATGTCA TTTGCAAAGT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACACGCAG AGCCAACGCT GAGCCCTGCG GTCACTCTAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAACCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TATGTGTTCC ACTACCAAAA ATATTCATTA AGCGTCAAGC CCCACTGAAG 
GTATCTCTTC TTCAGGACCT CAAAGACTTT TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAGA GACTTGCATC CTTGAAAACG GATACATTTA GCAAAACTAG AGAGGAAAAG 
ATGGAAGATA TCTTTGCACA AAAGGAGATG GAAGAGGGTG AGTTTAAGAA CTGGACAGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTGGATACCC CTCAGCAACT GCAGTCCATT 
TTTGAGTCAC TGATTGCCAA GAAGCAAAGC CTCTGTGAGG TGCTCCAGGC GTGGAACAGC 
AGGTTGCAGG ACCTCTTCCA GCAGGAAAAA GGTAGAAAGA GGCCTTCAGT TCCTCCCAGT 
CCTGGGAGAC TGAGACAAGG TGAAGAAAGC AAGATAAATG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAATGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGCCAGAGCT CTACGAGCTC CACCCACCTC CAGCTGCCCA CTCCTCCCGA GGCCCTGGCC 
GAGCAGGTAG TGGGAGGGCC GACTGATCTG GATTCAGCCA GTGGCTCTGA AGATGTATTT 
GATGGTCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TACAATCCCA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGCAATGC CTTAGAGGAA 
TTGTCCAAAG CAACTCTGCG GAACAGTGCT GAAGAAGGGC TCCCAGCCAA TAGTGCTTTA 
GATAACAGAC CTAAGAGTAG CAGCCCTATT AGACTACCTG AAATCAGTGG AGGACAGACA 
AACCGCACAG TAGAAGCAGA ACCTCAGCCA ACCAAAAAAG CTTCAGGAAT GTTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGCCCTGAT CTGTCTTCCC AGAAGAGGGA GACCTTGCGA 
GGGGCAGACA GTGCTTACTA CCAGGTTGGG CAGGCCGGCA AGGAGGGGTT GGAGAGTCAA 
GGCCTGGAGC CTCAAGATGA AGTAGATGGA GGAGATACAC AGAAGAAACA ACTCACAAAT 
CCTCATGTGG AACTTCAATT TTCTGATGCT AATGCCAAGT TTTACTGTCG GCTGTACTAC 
GCGGGAGAGT TCCACAAGAT GCGTGAAGTG ATTCTGGGCA GCAGTGAGGA GGAATTCATC 
CGTTCCCTTT CTCACTCATC TCCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACCG AAGATGATAG ATTCATTCTG AAGCAAATGC CTCGTTTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCACA CTACTTCAAT TATATCACAA ATGCTGTTCA ACAAAAGAGG 
CCCACCGCCT TGGCTAAAAT TCTTGGAGTT TACAGAATTG GTTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTAAAAAC TGACACTGGG 
AAAGAGAGCT GTGATGTGGT TCTGTTGGAT GAAAACCTCC TAAAGATGGT TCGAGACAAC 
CCTCTCTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA CAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTCTGC TGGTTGGGCG AGATGACACT 
AGCAATGAGC TTGTGGTTGG CATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTTGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACTGTG 
GTCTCTCCAG AGTTGTATAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035216.2
CDS	159..6317
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	NM_011086.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011086.2

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGAGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCC TACTCCAGCA GTTGCTTCAA 
AACGAGTCAT TGTCATCGTC TTGGAGGGAC ATCATTGTGT CACTAGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGACTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC CTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCTCAGGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACTTGGGC 
ACTTGCCACA AATTTTATAT GCAGATATTT CAGCTGCCTA ATGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTCC ACAGCATCTA GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCTTT TCTCATGGAT GAGTTCGCTA TGCCTCCAAC ATTAATGCAA 
AGCCCTTCAT TCCATCTTCT GACGGAGGGA CGAGGTGAAG AGGGAGCCTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC TCAGGATCCT GAGTGCCCTC GTGAGGCCCT GTCTTCTGAG 
GATAGCACTT TGTTGGAATC AAGGACTGTG CTAGAGAAGG GTGAACTAGA CAATAAAAGT 
ATTCCACAAG CTGTTGCCTC TTTGAAGCAT CAAGATTATA CCACCCCCAC TTGCCCAGCA 
GGTATTCCCT GTGCTCTTTT TGCATTGGTA CCAGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CCGTAGGAAA TGAACAGCCA GAGACTTCAC AGCAAACGGA TGAGCAACAG 
GATCCCAAAA GCCAGATGAA AGCTTTTAGA GACCCTTTAC AGGATGACAC TGGAATGTAC 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT CAACGAAAGA CTTATGCCTT GACATTTAAA 
CAGGAGTTAA AAGATGTAAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGATGCTCA ACTCGAGATT ATTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAACAA AGAGGTGAAG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGCAGTG TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAGCT AGTGAGCACC AGGATTGCTG AACATCTGGG TGACAGCCAG 
ACCTTGGGTA GAATGCTAGC TGATTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCCT TTGTCCATTC AAAAGATGCA TCATGTACTT CAGGTGGCAA ATCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATTCCAAGTG ATGTAATATG GCCAACAAAG 
GTGGACTGTC TGAACCCTGC TAACCACCAG AGGCTCTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC CAGTGCTTGT GTCAGTCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGGATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CGAGCATGTT CTGTGACACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATTCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA TTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCTATGTCA TTTGCAAAGT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACACGCAG AGCCAACGCT GAGCCCTGCG GTCACTCTAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAACCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TATGTGTTCC ACTACCAAAA ATATTCATTA AGCGTCAAGC CCCACTGAAG 
GTATCTCTTC TTCAGGACCT CAAAGACTTT TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAGA GACTTGCATC CTTGAAAACG GATACATTTA GCAAAACTAG AGAGGAAAAG 
ATGGAAGATA TCTTTGCACA AAAGGAGATG GAAGAGGGTG AGTTTAAGAA CTGGACAGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTGGATACCC CTCAGCAACT GCAGTCCATT 
TTTGAGTCAC TGATTGCCAA GAAGCAAAGC CTCTGTGAGG TGCTCCAGGC GTGGAACAGC 
AGGTTGCAGG ACCTCTTCCA GCAGGAAAAA GGTAGAAAGA GGCCTTCAGT TCCTCCCAGT 
CCTGGGAGAC TGAGACAAGG TGAAGAAAGC AAGATAAATG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAATGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGCCAGAGCT CTACGAGCTC CACCCACCTC CAGCTGCCCA CTCCTCCCGA GGCCCTGGCC 
GAGCAGGTAG TGGGAGGGCC GACTGATCTG GATTCAGCCA GTGGCTCTGA AGATGTATTT 
GATGGTCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TACAATCCCA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGCAATGC CTTAGAGGAA 
TTGTCCAAAG CAACTCTGCG GAACAGTGCT GAAGAAGGGC TCCCAGCCAA TAGTGCTTTA 
GATAACAGAC CTAAGAGTAG CAGCCCTATT AGACTACCTG AAATCAGTGG AGGACAGACA 
AACCGCACAG TAGAAGCAGA ACCTCAGCCA ACCAAAAAAG CTTCAGGAAT GTTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGCCCTGAT CTGTCTTCCC AGAAGAGGGA GACCTTGCGA 
GGGGCAGACA GTGCTTACTA CCAGGTTGGG CAGGCCGGCA AGGAGGGGTT GGAGAGTCAA 
GGCCTGGAGC CTCAAGATGA AGTAGATGGA GGAGATACAC AGAAGAAACA ACTCACAAAT 
CCTCATGTGG AACTTCAATT TTCTGATGCT AATGCCAAGT TTTACTGTCG GCTGTACTAC 
GCGGGAGAGT TCCACAAGAT GCGTGAAGTG ATTCTGGGCA GCAGTGAGGA GGAATTCATC 
CGTTCCCTTT CTCACTCATC TCCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACCG AAGATGATAG ATTCATTCTG AAGCAAATGC CTCGTTTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCACA CTACTTCAAT TATATCACAA ATGCTGTTCA ACAAAAGAGG 
CCCACCGCCT TGGCTAAAAT TCTTGGAGTT TACAGAATTG GTTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTAAAAAC TGACACTGGG 
AAAGAGAGCT GTGATGTGGT TCTGTTGGAT GAAAACCTCC TAAAGATGGT TCGAGACAAC 
CCTCTCTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA CAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTCTGC TGGTTGGGCG AGATGACACT 
AGCAATGAGC TTGTGGTTGG CATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTTGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACTGTG 
GTCTCTCCAG AGTTGTATAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( NM_001310624.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297553.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant 1, mRNA.

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CloneID	OMu27303
Clone ID Related Accession (Same CDS sequence)	XM_006495781.3 , XM_006495781.4 , NM_001310624.1
Accession Version	NM_001310624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY271210.1, AK173077.1 and AC164079.11. On Jul 16, 2015 this sequence version replaced XM_006495780.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AGAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAGTTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA CTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ATCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACATTAA TGCAAAGCCC TTCATTCCAT CTTCTGACGG AGGGACGAGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGAGGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT TCCCTGTGCT CTTTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCCGTA GGAAATGAAC AGCCAGAGAC TTCACAGCAA 
ACGGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TTTACAGGAT 
GACACTGGAA TGTACGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA GTTAAAAGAT GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAGCTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCCTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTA 
ATATGGCCAA CAAAGGTGGA CTGTCTGAAC CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GGATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAAG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACTAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGC 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGCGGAACA GTGCTGAAGA AGGGCTCCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGACT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGTAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGAATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCCCAGAAG 
AGGGAGACCT TGCGAGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC CGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ATGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAC AAGATGCGTG AAGTGATTCT GGGCAGCAGT 
GAGGAGGAAT TCATCCGTTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGTTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297553.1
CDS	159..6452
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	NM_001310624.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310624.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AGAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAGTTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA CTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ATCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACATTAA TGCAAAGCCC TTCATTCCAT CTTCTGACGG AGGGACGAGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGAGGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT TCCCTGTGCT CTTTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCCGTA GGAAATGAAC AGCCAGAGAC TTCACAGCAA 
ACGGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TTTACAGGAT 
GACACTGGAA TGTACGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA GTTAAAAGAT GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAGCTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCCTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTA 
ATATGGCCAA CAAAGGTGGA CTGTCTGAAC CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GGATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAAG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACTAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGC 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGCGGAACA GTGCTGAAGA AGGGCTCCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGACT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGTAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGAATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCCCAGAAG 
AGGGAGACCT TGCGAGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC CGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ATGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAC AAGATGCGTG AAGTGATTCT GGGCAGCAGT 
GAGGAGGAAT TCATCCGTTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGTTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495776.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495839.1 Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

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CloneID	OMu27301
Clone ID Related Accession (Same CDS sequence)	XM_006495776.3 , XM_006495777.3 , XM_006495777.4
Accession Version	XM_006495776.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAAGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACTA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGCTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGCAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGC GGAACAGTGC TGAAGAAGGG CTCCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGACTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GTAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGAA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCC CAGAAGAGGG AGACCTTGCG AGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCCGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCACAAGA TGCGTGAAGT GATTCTGGGC AGCAGTGAGG AGGAATTCAT CCGTTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495839.1
CDS	204..6533
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495776.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495776.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAAGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACTA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGCTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGCAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGC GGAACAGTGC TGAAGAAGGG CTCCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGACTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GTAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGAA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCC CAGAAGAGGG AGACCTTGCG AGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCCGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCACAAGA TGCGTGAAGT GATTCTGGGC AGCAGTGAGG AGGAATTCAT CCGTTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495777.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495840.1 Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X2, mRNA.

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CloneID	OMu27301
Clone ID Related Accession (Same CDS sequence)	XM_006495776.3 , XM_006495777.3 , XM_006495777.4
Accession Version	XM_006495777.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAAGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACTA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGCTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGCAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGC GGAACAGTGC TGAAGAAGGG CTCCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGACTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GTAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGAA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCC CAGAAGAGGG AGACCTTGCG AGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCCGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCACAAGA TGCGTGAAGT GATTCTGGGC AGCAGTGAGG AGGAATTCAT CCGTTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495840.1
CDS	149..6478
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495777.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495777.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAGAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AGTTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGACTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCATCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CATTAATGCA AAGCCCTTCA 
TTCCATCTTC TGACGGAGGG ACGAGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA GGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATTCCC 
TGTGCTCTTT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCCGTAGGAA ATGAACAGCC AGAGACTTCA CAGCAAACGG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTTTA CAGGATGACA CTGGAATGTA CGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAGTTA 
AAAGATGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCC 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTAATAT GGCCAACAAA GGTGGACTGT 
CTGAACCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGGAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAAGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACTA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGCTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGCAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGC GGAACAGTGC TGAAGAAGGG CTCCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGACTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GTAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGAA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCC CAGAAGAGGG AGACCTTGCG AGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCCGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCACAAGA TGCGTGAAGT GATTCTGGGC AGCAGTGAGG AGGAATTCAT CCGTTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495779.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495842.1 Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X3, mRNA.

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CloneID	OMu27302
Clone ID Related Accession (Same CDS sequence)	XM_006495779.3 , XM_006495779.4 , XM_030252267.1
Accession Version	XM_006495779.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAGAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAGT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGACTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCATCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACAT TAATGCAAAG CCCTTCATTC 
CATCTTCTGA CGGAGGGACG AGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGAGGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATTCCCTGT 
GCTCTTTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCC 
GTAGGAAATG AACAGCCAGA GACTTCACAG CAAACGGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTTTACAG GATGACACTG GAATGTACGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAGTTAAAA 
GATGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCCTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTAATATGGC CAACAAAGGT GGACTGTCTG 
AACCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGGATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AAGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACTAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGCTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGCAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGCGGA ACAGTGCTGA AGAAGGGCTC CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG ACTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGTA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGAATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCCCAG AAGAGGGAGA CCTTGCGAGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCCGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CACAAGATGC GTGAAGTGAT TCTGGGCAGC AGTGAGGAGG AATTCATCCG TTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495842.1
CDS	204..6530
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495779.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495779.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CTACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCAGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGCTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACA GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGGAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA TCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCTGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAGAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAGT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGACTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCATCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACAT TAATGCAAAG CCCTTCATTC 
CATCTTCTGA CGGAGGGACG AGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGAGGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATTCCCTGT 
GCTCTTTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCC 
GTAGGAAATG AACAGCCAGA GACTTCACAG CAAACGGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTTTACAG GATGACACTG GAATGTACGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAGTTAAAA 
GATGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCCTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTAATATGGC CAACAAAGGT GGACTGTCTG 
AACCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGGATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AAGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACTAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGCTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGCAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGCGGA ACAGTGCTGA AGAAGGGCTC CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG ACTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGTA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGAATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCCCAG AAGAGGGAGA CCTTGCGAGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCCGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CACAAGATGC GTGAAGTGAT TCTGGGCAGC AGTGAGGAGG AATTCATCCG TTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495781.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495844.1 Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X4, mRNA.

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CloneID	OMu27303
Clone ID Related Accession (Same CDS sequence)	XM_006495781.3 , XM_006495781.4 , NM_001310624.1
Accession Version	XM_006495781.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AGAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAGTTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA CTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ATCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACATTAA TGCAAAGCCC TTCATTCCAT CTTCTGACGG AGGGACGAGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGAGGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT TCCCTGTGCT CTTTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCCGTA GGAAATGAAC AGCCAGAGAC TTCACAGCAA 
ACGGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TTTACAGGAT 
GACACTGGAA TGTACGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA GTTAAAAGAT GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAGCTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCCTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTA 
ATATGGCCAA CAAAGGTGGA CTGTCTGAAC CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GGATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAAG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACTAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGC 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGCGGAACA GTGCTGAAGA AGGGCTCCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGACT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGTAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGAATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCCCAGAAG 
AGGGAGACCT TGCGAGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC CGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ATGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAC AAGATGCGTG AAGTGATTCT GGGCAGCAGT 
GAGGAGGAAT TCATCCGTTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGTTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495844.1
CDS	149..6442
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495781.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495781.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCTA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CAGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GCTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACAGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG GAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGATCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CTGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AGAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAGTTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA CTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ATCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACATTAA TGCAAAGCCC TTCATTCCAT CTTCTGACGG AGGGACGAGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGAGGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT TCCCTGTGCT CTTTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCCGTA GGAAATGAAC AGCCAGAGAC TTCACAGCAA 
ACGGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TTTACAGGAT 
GACACTGGAA TGTACGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA GTTAAAAGAT GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAGCTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCCTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTA 
ATATGGCCAA CAAAGGTGGA CTGTCTGAAC CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GGATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAAG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACTAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGC 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGCGGAACA GTGCTGAAGA AGGGCTCCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGACT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGTAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGAATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCCCAGAAG 
AGGGAGACCT TGCGAGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC CGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ATGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAC AAGATGCGTG AAGTGATTCT GGGCAGCAGT 
GAGGAGGAAT TCATCCGTTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGTTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006495782.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495845.1 Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X5, mRNA.

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CloneID	OMu27304
Clone ID Related Accession (Same CDS sequence)	XM_006495782.3 , XM_006495782.4
Accession Version	XM_006495782.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGAGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCCTACTCCA GCAGTTGCTT 
CAAAACGAGT CATTGTCATC GTCTTGGAGG GACATCATTG TGTCACTAGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGACT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACCTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCTCA GGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACTTG 
GGCACTTGCC ACAAATTTTA TATGCAGATA TTTCAGCTGC CTAATGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAT CTAGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC TTTTCTCATG GATGAGTTCG CTATGCCTCC AACATTAATG 
CAAAGCCCTT CATTCCATCT TCTGACGGAG GGACGAGGTG AAGAGGGAGC CTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCTCAGGAT CCTGAGTGCC CTCGTGAGGC CCTGTCTTCT 
GAGGATAGCA CTTTGTTGGA ATCAAGGACT GTGCTAGAGA AGGGTGAACT AGACAATAAA 
AGTATTCCAC AAGCTGTTGC CTCTTTGAAG CATCAAGATT ATACCACCCC CACTTGCCCA 
GCAGGTATTC CCTGTGCTCT TTTTGCATTG GTACCAGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCCGTAGG AAATGAACAG CCAGAGACTT CACAGCAAAC GGATGAGCAA 
CAGGATCCCA AAAGCCAGAT GAAAGCTTTT AGAGACCCTT TACAGGATGA CACTGGAATG 
TACGTTACTG AGGAAGTCAC CTCCTCTGAA GATCAACGAA AGACTTATGC CTTGACATTT 
AAACAGGAGT TAAAAGATGT AATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGATGC TCAACTCGAG ATTATTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA CAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGCA GTGTTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACCAGGATTG CTGAACATCT GGGTGACAGC 
CAGACCTTGG GTAGAATGCT AGCTGATTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CCTTTGTCCA TTCAAAAGAT GCATCATGTA CTTCAGGTGG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTAAT ATGGCCAACA 
AAGGTGGACT GTCTGAACCC TGCTAACCAC CAGAGGCTCT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCT TGTGTCAGTC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGG ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCGAGCAT GTTCTGTGAC ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATT CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCTATG TCATTTGCAA AGTACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACACG CAGAGCCAAC GCTGAGCCCT GCGGTCACTC TATCCACCAT 
GATTATCACC AGTATTTCTC TTATAACCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTATGTGT TCCACTACCA AAAATATTCA TTAAGCGTCA AGCCCCACTG 
AAGGTATCTC TTCTTCAGGA CCTCAAAGAC TTTTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AGAGACTTGC ATCCTTGAAA ACGGATACAT TTAGCAAAAC TAGAGAGGAA 
AAGATGGAAG ATATCTTTGC ACAAAAGGAG ATGGAAGAGG GTGAGTTTAA GAACTGGACA 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTGGATA CCCCTCAGCA ACTGCAGTCC 
ATTTTTGAGT CACTGATTGC CAAGAAGCAA AGCCTCTGTG AGGTGCTCCA GGCGTGGAAC 
AGCAGGTTGC AGGACCTCTT CCAGCAGGAA AAAGGTAGAA AGAGGCCTTC AGTTCCTCCC 
AGTCCTGGGA GACTGAGACA AGGTGAAGAA AGCAAGATAA ATGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAATGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGCCAGA GCTCTACGAG CTCCACCCAC CTCCAGCTGC CCACTCCTCC CGAGGCCCTG 
GCCGAGCAGG TAGTGGGAGG GCCGACTGAT CTGGATTCAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGTC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTACAATC CCATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGCAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GCGGAACAGT GCTGAAGAAG GGCTCCCAGC CAATAGTGCT 
TTAGATAACA GACCTAAGAG TAGCAGCCCT ATTAGACTAC CTGAAATCAG TGGAGGACAG 
ACAAACCGCA CAGTAGAAGC AGAACCTCAG CCAACCAAAA AAGCTTCAGG AATGTTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CCCAGAAGAG GGAGACCTTG 
CGAGGGGCAG ACAGTGCTTA CTACCAGGTT GGGCAGGCCG GCAAGGAGGG GTTGGAGAGT 
CAAGGCCTGG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTCACA 
AATCCTCATG TGGAACTTCA ATTTTCTGAT GCTAATGCCA AGTTTTACTG TCGGCTGTAC 
TACGCGGGAG AGTTCCACAA GATGCGTGAA GTGATTCTGG GCAGCAGTGA GGAGGAATTC 
ATCCGTTCCC TTTCTCACTC ATCTCCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CCGAAGATGA TAGATTCATT CTGAAGCAAA TGCCTCGTTT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC ACACTACTTC AATTATATCA CAAATGCTGT TCAACAAAAG 
AGGCCCACCG CCTTGGCTAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACTGACACT 
GGGAAAGAGA GCTGTGATGT GGTTCTGTTG GATGAAAACC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTCT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCACAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTC TGCTGGTTGG GCGAGATGAC 
ACTAGCAATG AGCTTGTGGT TGGCATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTTGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACT 
GTGGTCTCTC CAGAGTTGTA TAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495845.1
CDS	204..6365
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKSGNKTESDEERGLIPSDVIWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRLPEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEEFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006495782.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide kinase, FYVE finger containing (Pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495782.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GTTTTAACAA AGAGCGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCTACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCAGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGCTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACAGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGGAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGATCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCTGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGAGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCCTACTCCA GCAGTTGCTT 
CAAAACGAGT CATTGTCATC GTCTTGGAGG GACATCATTG TGTCACTAGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGACT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACCTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCTCA GGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACTTG 
GGCACTTGCC ACAAATTTTA TATGCAGATA TTTCAGCTGC CTAATGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAT CTAGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC TTTTCTCATG GATGAGTTCG CTATGCCTCC AACATTAATG 
CAAAGCCCTT CATTCCATCT TCTGACGGAG GGACGAGGTG AAGAGGGAGC CTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCTCAGGAT CCTGAGTGCC CTCGTGAGGC CCTGTCTTCT 
GAGGATAGCA CTTTGTTGGA ATCAAGGACT GTGCTAGAGA AGGGTGAACT AGACAATAAA 
AGTATTCCAC AAGCTGTTGC CTCTTTGAAG CATCAAGATT ATACCACCCC CACTTGCCCA 
GCAGGTATTC CCTGTGCTCT TTTTGCATTG GTACCAGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCCGTAGG AAATGAACAG CCAGAGACTT CACAGCAAAC GGATGAGCAA 
CAGGATCCCA AAAGCCAGAT GAAAGCTTTT AGAGACCCTT TACAGGATGA CACTGGAATG 
TACGTTACTG AGGAAGTCAC CTCCTCTGAA GATCAACGAA AGACTTATGC CTTGACATTT 
AAACAGGAGT TAAAAGATGT AATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGATGC TCAACTCGAG ATTATTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA CAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGCA GTGTTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACCAGGATTG CTGAACATCT GGGTGACAGC 
CAGACCTTGG GTAGAATGCT AGCTGATTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CCTTTGTCCA TTCAAAAGAT GCATCATGTA CTTCAGGTGG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTAAT ATGGCCAACA 
AAGGTGGACT GTCTGAACCC TGCTAACCAC CAGAGGCTCT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCT TGTGTCAGTC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGG ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCGAGCAT GTTCTGTGAC ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATT CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCTATG TCATTTGCAA AGTACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACACG CAGAGCCAAC GCTGAGCCCT GCGGTCACTC TATCCACCAT 
GATTATCACC AGTATTTCTC TTATAACCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTATGTGT TCCACTACCA AAAATATTCA TTAAGCGTCA AGCCCCACTG 
AAGGTATCTC TTCTTCAGGA CCTCAAAGAC TTTTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AGAGACTTGC ATCCTTGAAA ACGGATACAT TTAGCAAAAC TAGAGAGGAA 
AAGATGGAAG ATATCTTTGC ACAAAAGGAG ATGGAAGAGG GTGAGTTTAA GAACTGGACA 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTGGATA CCCCTCAGCA ACTGCAGTCC 
ATTTTTGAGT CACTGATTGC CAAGAAGCAA AGCCTCTGTG AGGTGCTCCA GGCGTGGAAC 
AGCAGGTTGC AGGACCTCTT CCAGCAGGAA AAAGGTAGAA AGAGGCCTTC AGTTCCTCCC 
AGTCCTGGGA GACTGAGACA AGGTGAAGAA AGCAAGATAA ATGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAATGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGCCAGA GCTCTACGAG CTCCACCCAC CTCCAGCTGC CCACTCCTCC CGAGGCCCTG 
GCCGAGCAGG TAGTGGGAGG GCCGACTGAT CTGGATTCAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGTC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTACAATC CCATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGCAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GCGGAACAGT GCTGAAGAAG GGCTCCCAGC CAATAGTGCT 
TTAGATAACA GACCTAAGAG TAGCAGCCCT ATTAGACTAC CTGAAATCAG TGGAGGACAG 
ACAAACCGCA CAGTAGAAGC AGAACCTCAG CCAACCAAAA AAGCTTCAGG AATGTTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CCCAGAAGAG GGAGACCTTG 
CGAGGGGCAG ACAGTGCTTA CTACCAGGTT GGGCAGGCCG GCAAGGAGGG GTTGGAGAGT 
CAAGGCCTGG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTCACA 
AATCCTCATG TGGAACTTCA ATTTTCTGAT GCTAATGCCA AGTTTTACTG TCGGCTGTAC 
TACGCGGGAG AGTTCCACAA GATGCGTGAA GTGATTCTGG GCAGCAGTGA GGAGGAATTC 
ATCCGTTCCC TTTCTCACTC ATCTCCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CCGAAGATGA TAGATTCATT CTGAAGCAAA TGCCTCGTTT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC ACACTACTTC AATTATATCA CAAATGCTGT TCAACAAAAG 
AGGCCCACCG CCTTGGCTAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACTGACACT 
GGGAAAGAGA GCTGTGATGT GGTTCTGTTG GATGAAAACC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTCT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCACAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTC TGCTGGTTGG GCGAGATGAC 
ACTAGCAATG AGCTTGTGGT TGGCATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTTGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACT 
GTGGTCTCTC CAGAGTTGTA TAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Lipid Kinase PIKfyve Coordinates the Neutrophil Immune Response through the Activation of the Rac GTPase.
Journal of immunology (Baltimore, Md. : 1950)199(6)2096-2105(2017 Sep)
Dayam RM,Sun CX,Choy CH,Mancuso G,Glogauer M,Botelho RJ

Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.
The EMBO journal36(12)1707-1718(2017 06)
Kawasaki T,Ito K,Miyata H,Akira S,Kawai T

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