Rock1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Rock1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rock1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu54439 XM_006525725.2
Latest version!
Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu20908 NM_009071.2
Latest version!
Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OMu54439 XM_006525725.1 Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu54439
    Clone ID Related Accession (Same CDS sequence) XM_006525725.1 , XM_006525725.2
    Accession Version XM_006525725.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG
    GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG
    GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT
    TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC
    CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG
    CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT
    ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC
    TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA
    GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA
    GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT
    GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT
    AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG
    GATAAAAGCT TGGAAAGTTT ACAGAAAACA ATCTACAAGC TTGAAGAGCA ACTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACTT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAATCTAGA ATCTGCAGTG
    TCTCAAATTG AGAAAGAAAA GATGTTGCTA CAACATAGAA TTAATGAGTA CCAAAGAAAA
    GTGGAACAGG AAAATGAGAA GAGAAGAAAT ATAGAAAATG AAGTTTCTAC ACTAAAGGAC
    CAGTTAGAAG ACTTACGGAA AGCAAGTCAG ACCTCACAGC TTGCTAATGA GAAGCTGACA
    CAGTTACAGA AGCAGTTAGA AGAAGCGAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGTCA CACAGAGATG AGCAAGTCAA TTAGTCAGCT AGAGTCCCTG
    AACAGAGAAT TACAGGAAAG AAACAGAATT TTAGAGAATT CCAAGTCACA AGCAGACAAG
    GATTACTACC AGCTGCAAGC TGTATTGGAA GCTGAGCGAA GAGACAGAGG GCATGATTCT
    GAGATGATTG GAGACCTTCA AGCACGAATT ACATCTTTAC AAGAAGAAGT GAAACATCTC
    AAACATAATC TTGAAAGAGT GGAAGGAGAA AGAAAAGAGG CTCAAGACAT GCTTAATCAT
    TCAGAAAAGG AAAAGAATAA TTTGGAGATA GATTTGAATT ACAAGCTCAA GTCTATACAA
    CAGCGGTTAG AACAAGAAGT AAATGAACAT AAAGTCACCA AAGCACGCCT AACTGACAAG
    CACCAATCTA TCGAAGAGGC AAAGTCTGTT GCCATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCAGAA AATAGAGTTG TTGAGACTGA GAAACAATGT
    TCCATGCTAG ATGTTGACCT GAAACAGTCT CAGCAGAAGC TAGAACATTT GACCGAAAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTCGCCCTGC AGCTGGAACA GGAATCAAAT
    AAACGGCTCT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTTTAGAAA AACAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTGTTA
    GAATTTGAGT TAGCTCAGCT TACAAAGCAA TATAGGGGAA ATGAAGGGCA AATGCGGGAG
    TTACAAGATC AACTTGAAGC TGAGCAGTAT TTCTCTACAC TTTATAAAAC CCAAGTAAAG
    GAGCTTAAAG AAGAAATTGA AGAAAAAAAC CGAGAAAATT TAAGGAAAAT ACAGGAACTG
    CAAAGTGAAA AAGAAACTCT CTCTACTCAG TTGGATTTAG CAGAAACAAA AGCTGAGTCT
    GAGCAGCTGG CGCGAGGGAT TCTGGAAGAA CAGTATTTTG AGTTGACTCA GGAAAGCAAG
    AAAGCTGCTT CAAGAAATAG ACAAGAGATT ACAGATAAAG ATCATACTGT TAGTCGGCTT
    GAAGAAACAA ACAGTGTGCT AACCAAAGAT ATTGAAATGC TAAGAAAAGA AAATGAAGAG
    CTAAATGAAA GAATGAGGAC AGCAGAAGAA GAATATAAAT TGAAGAAGGA GGAAGAAATA
    AATAATCTTA AGGCTGCTTT TGAAAAGAAT ATCAGTACCG AACGGACCCT TAAAACACAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGCAAAGATT TTAAAATTGA TCGAAAGAAA
    GCTAATACAC AAGATTTAAG AAAAAAAGAA AAGGAAAATA GAAAGCTACA ACTGGAACTT
    AACCAAGAGA GAGAAAAATT TAACCAAATG GTGGTGAAAC ACCAGAAGGA GCTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACA CATAGAAATG AACTTCAAAT GCAGTTGGCT
    AGCAAAGAGA GTGATATTGA GCAGTTGCGG GCAAAACTTT TGGACCTTTC GGATTCAACT
    AGTGTTGCTA GTTTTCCTAG TGCAGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCTGTACC AAATAGAGGA AATATCAAAA GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAGATCTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAGTCCAT CTATGGTGTT AGACATAGAT AAATTGTTTC ATGTCCGACC TGTAACCCAA
    GGAGATGTGT ACAGAGCAGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATATAGAA GTGGAACCGG TTCAACAAGG TGAAAAAACT
    AATTTCCAGA ATCACAAAGG CCATGAATTC ATTCCTACCC TCTACCACTT TCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TTTGTGGCAT GTCTTTAAGC CACCACCTGC CCTAGAGTGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAC CATCTAGACA AGAAAGAGGA CTTGATTTCC
    CCGTGCAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAGTG GGTTACTCAT CTAGTAAAGA AGATTCCTAA GAACCCACCA
    TCTGGCTTTG TCCGTGCCTC TCCTCGAACG CTGTCAACAA GATCCACTGC AAATCAGTCT
    TTCCGCAAAG TAGTCAAAAA TACATCTGGA AAAACTAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006525788.1
    CDS729..4790
    Translation

    Target ORF information:

    RefSeq Version XM_006525725.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006525725.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG
    GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG
    GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT
    TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC
    CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG
    CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT
    ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC
    TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA
    GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA
    GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT
    GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT
    AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG
    GATAAAAGCT TGGAAAGTTT ACAGAAAACA ATCTACAAGC TTGAAGAGCA ACTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACTT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAATCTAGA ATCTGCAGTG
    TCTCAAATTG AGAAAGAAAA GATGTTGCTA CAACATAGAA TTAATGAGTA CCAAAGAAAA
    GTGGAACAGG AAAATGAGAA GAGAAGAAAT ATAGAAAATG AAGTTTCTAC ACTAAAGGAC
    CAGTTAGAAG ACTTACGGAA AGCAAGTCAG ACCTCACAGC TTGCTAATGA GAAGCTGACA
    CAGTTACAGA AGCAGTTAGA AGAAGCGAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGTCA CACAGAGATG AGCAAGTCAA TTAGTCAGCT AGAGTCCCTG
    AACAGAGAAT TACAGGAAAG AAACAGAATT TTAGAGAATT CCAAGTCACA AGCAGACAAG
    GATTACTACC AGCTGCAAGC TGTATTGGAA GCTGAGCGAA GAGACAGAGG GCATGATTCT
    GAGATGATTG GAGACCTTCA AGCACGAATT ACATCTTTAC AAGAAGAAGT GAAACATCTC
    AAACATAATC TTGAAAGAGT GGAAGGAGAA AGAAAAGAGG CTCAAGACAT GCTTAATCAT
    TCAGAAAAGG AAAAGAATAA TTTGGAGATA GATTTGAATT ACAAGCTCAA GTCTATACAA
    CAGCGGTTAG AACAAGAAGT AAATGAACAT AAAGTCACCA AAGCACGCCT AACTGACAAG
    CACCAATCTA TCGAAGAGGC AAAGTCTGTT GCCATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCAGAA AATAGAGTTG TTGAGACTGA GAAACAATGT
    TCCATGCTAG ATGTTGACCT GAAACAGTCT CAGCAGAAGC TAGAACATTT GACCGAAAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTCGCCCTGC AGCTGGAACA GGAATCAAAT
    AAACGGCTCT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTTTAGAAA AACAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTGTTA
    GAATTTGAGT TAGCTCAGCT TACAAAGCAA TATAGGGGAA ATGAAGGGCA AATGCGGGAG
    TTACAAGATC AACTTGAAGC TGAGCAGTAT TTCTCTACAC TTTATAAAAC CCAAGTAAAG
    GAGCTTAAAG AAGAAATTGA AGAAAAAAAC CGAGAAAATT TAAGGAAAAT ACAGGAACTG
    CAAAGTGAAA AAGAAACTCT CTCTACTCAG TTGGATTTAG CAGAAACAAA AGCTGAGTCT
    GAGCAGCTGG CGCGAGGGAT TCTGGAAGAA CAGTATTTTG AGTTGACTCA GGAAAGCAAG
    AAAGCTGCTT CAAGAAATAG ACAAGAGATT ACAGATAAAG ATCATACTGT TAGTCGGCTT
    GAAGAAACAA ACAGTGTGCT AACCAAAGAT ATTGAAATGC TAAGAAAAGA AAATGAAGAG
    CTAAATGAAA GAATGAGGAC AGCAGAAGAA GAATATAAAT TGAAGAAGGA GGAAGAAATA
    AATAATCTTA AGGCTGCTTT TGAAAAGAAT ATCAGTACCG AACGGACCCT TAAAACACAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGCAAAGATT TTAAAATTGA TCGAAAGAAA
    GCTAATACAC AAGATTTAAG AAAAAAAGAA AAGGAAAATA GAAAGCTACA ACTGGAACTT
    AACCAAGAGA GAGAAAAATT TAACCAAATG GTGGTGAAAC ACCAGAAGGA GCTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACA CATAGAAATG AACTTCAAAT GCAGTTGGCT
    AGCAAAGAGA GTGATATTGA GCAGTTGCGG GCAAAACTTT TGGACCTTTC GGATTCAACT
    AGTGTTGCTA GTTTTCCTAG TGCAGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCTGTACC AAATAGAGGA AATATCAAAA GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAGATCTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAGTCCAT CTATGGTGTT AGACATAGAT AAATTGTTTC ATGTCCGACC TGTAACCCAA
    GGAGATGTGT ACAGAGCAGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATATAGAA GTGGAACCGG TTCAACAAGG TGAAAAAACT
    AATTTCCAGA ATCACAAAGG CCATGAATTC ATTCCTACCC TCTACCACTT TCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TTTGTGGCAT GTCTTTAAGC CACCACCTGC CCTAGAGTGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAC CATCTAGACA AGAAAGAGGA CTTGATTTCC
    CCGTGCAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAGTG GGTTACTCAT CTAGTAAAGA AGATTCCTAA GAACCCACCA
    TCTGGCTTTG TCCGTGCCTC TCCTCGAACG CTGTCAACAA GATCCACTGC AAATCAGTCT
    TTCCGCAAAG TAGTCAAAAA TACATCTGGA AAAACTAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20908
    Clone ID Related Accession (Same CDS sequence) NM_009071.2
    Accession Version NM_009071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product rho-associated protein kinase 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK149793.1, U58512.1, AA163962.1, AK077297.1 and DR754490.1. On Mar 31, 2007 this sequence version replaced NM_009071.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG
    GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG
    GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT
    TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC
    CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG
    CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT
    ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC
    TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA
    GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA
    GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT
    GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT
    AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG
    GATAAAAGCT TGCAGGAAAG TTTACAGAAA ACAATCTACA AGCTTGAAGA GCAACTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CTTAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAATCT AGAATCTGCA
    GTGTCTCAAA TTGAGAAAGA AAAGATGTTG CTACAACATA GAATTAATGA GTACCAAAGA
    AAAGTGGAAC AGGAAAATGA GAAGAGAAGA AATATAGAAA ATGAAGTTTC TACACTAAAG
    GACCAGTTAG AAGACTTACG GAAAGCAAGT CAGACCTCAC AGCTTGCTAA TGAGAAGCTG
    ACACAGTTAC AGAAGCAGTT AGAAGAAGCG AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GCTAGAGTCC
    CTGAACAGAG AATTACAGGA AAGAAACAGA ATTTTAGAGA ATTCCAAGTC ACAAGCAGAC
    AAGGATTACT ACCAGCTGCA AGCTGTATTG GAAGCTGAGC GAAGAGACAG AGGGCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCACGA ATTACATCTT TACAAGAAGA AGTGAAACAT
    CTCAAACATA ATCTTGAAAG AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT
    CATTCAGAAA AGGAAAAGAA TAATTTGGAG ATAGATTTGA ATTACAAGCT CAAGTCTATA
    CAACAGCGGT TAGAACAAGA AGTAAATGAA CATAAAGTCA CCAAAGCACG CCTAACTGAC
    AAGCACCAAT CTATCGAAGA GGCAAAGTCT GTTGCCATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCA GAAAATAGAG TTGTTGAGAC TGAGAAACAA
    TGTTCCATGC TAGATGTTGA CCTGAAACAG TCTCAGCAGA AGCTAGAACA TTTGACCGAA
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTCGCCC TGCAGCTGGA ACAGGAATCA
    AATAAACGGC TCTTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTTTAG AAAAACAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTG
    TTAGAATTTG AGTTAGCTCA GCTTACAAAG CAATATAGGG GAAATGAAGG GCAAATGCGG
    GAGTTACAAG ATCAACTTGA AGCTGAGCAG TATTTCTCTA CACTTTATAA AACCCAAGTA
    AAGGAGCTTA AAGAAGAAAT TGAAGAAAAA AACCGAGAAA ATTTAAGGAA AATACAGGAA
    CTGCAAAGTG AAAAAGAAAC TCTCTCTACT CAGTTGGATT TAGCAGAAAC AAAAGCTGAG
    TCTGAGCAGC TGGCGCGAGG GATTCTGGAA GAACAGTATT TTGAGTTGAC TCAGGAAAGC
    AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCATAC TGTTAGTCGG
    CTTGAAGAAA CAAACAGTGT GCTAACCAAA GATATTGAAA TGCTAAGAAA AGAAAATGAA
    GAGCTAAATG AAAGAATGAG GACAGCAGAA GAAGAATATA AATTGAAGAA GGAGGAAGAA
    ATAAATAATC TTAAGGCTGC TTTTGAAAAG AATATCAGTA CCGAACGGAC CCTTAAAACA
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGCAAAG ATTTTAAAAT TGATCGAAAG
    AAAGCTAATA CACAAGATTT AAGAAAAAAA GAAAAGGAAA ATAGAAAGCT ACAACTGGAA
    CTTAACCAAG AGAGAGAAAA ATTTAACCAA ATGGTGGTGA AACACCAGAA GGAGCTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACACATAGAA ATGAACTTCA AATGCAGTTG
    GCTAGCAAAG AGAGTGATAT TGAGCAGTTG CGGGCAAAAC TTTTGGACCT TTCGGATTCA
    ACTAGTGTTG CTAGTTTTCC TAGTGCAGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCTGT ACCAAATAGA GGAAATATCA AAAGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAGATC TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAGTC CATCTATGGT GTTAGACATA GATAAATTGT TTCATGTCCG ACCTGTAACC
    CAAGGAGATG TGTACAGAGC AGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATATA GAAGTGGAAC CGGTTCAACA AGGTGAAAAA
    ACTAATTTCC AGAATCACAA AGGCCATGAA TTCATTCCTA CCCTCTACCA CTTTCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTTTGTGG CATGTCTTTA AGCCACCACC TGCCCTAGAG
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GACCATCTAG ACAAGAAAGA GGACTTGATT
    TCCCCGTGCA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA GTGGGTTACT CATCTAGTAA AGAAGATTCC TAAGAACCCA
    CCATCTGGCT TTGTCCGTGC CTCTCCTCGA ACGCTGTCAA CAAGATCCAC TGCAAATCAG
    TCTTTCCGCA AAGTAGTCAA AAATACATCT GGAAAAACTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_033097.1
    CDS476..4540
    Translation

    Target ORF information:

    RefSeq Version NM_009071.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_009071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG
    GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG
    GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT
    TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC
    CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG
    CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT
    ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC
    TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA
    GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA
    GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT
    GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT
    AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG
    GATAAAAGCT TGCAGGAAAG TTTACAGAAA ACAATCTACA AGCTTGAAGA GCAACTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CTTAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAATCT AGAATCTGCA
    GTGTCTCAAA TTGAGAAAGA AAAGATGTTG CTACAACATA GAATTAATGA GTACCAAAGA
    AAAGTGGAAC AGGAAAATGA GAAGAGAAGA AATATAGAAA ATGAAGTTTC TACACTAAAG
    GACCAGTTAG AAGACTTACG GAAAGCAAGT CAGACCTCAC AGCTTGCTAA TGAGAAGCTG
    ACACAGTTAC AGAAGCAGTT AGAAGAAGCG AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GCTAGAGTCC
    CTGAACAGAG AATTACAGGA AAGAAACAGA ATTTTAGAGA ATTCCAAGTC ACAAGCAGAC
    AAGGATTACT ACCAGCTGCA AGCTGTATTG GAAGCTGAGC GAAGAGACAG AGGGCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCACGA ATTACATCTT TACAAGAAGA AGTGAAACAT
    CTCAAACATA ATCTTGAAAG AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT
    CATTCAGAAA AGGAAAAGAA TAATTTGGAG ATAGATTTGA ATTACAAGCT CAAGTCTATA
    CAACAGCGGT TAGAACAAGA AGTAAATGAA CATAAAGTCA CCAAAGCACG CCTAACTGAC
    AAGCACCAAT CTATCGAAGA GGCAAAGTCT GTTGCCATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCA GAAAATAGAG TTGTTGAGAC TGAGAAACAA
    TGTTCCATGC TAGATGTTGA CCTGAAACAG TCTCAGCAGA AGCTAGAACA TTTGACCGAA
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTCGCCC TGCAGCTGGA ACAGGAATCA
    AATAAACGGC TCTTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTTTAG AAAAACAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTG
    TTAGAATTTG AGTTAGCTCA GCTTACAAAG CAATATAGGG GAAATGAAGG GCAAATGCGG
    GAGTTACAAG ATCAACTTGA AGCTGAGCAG TATTTCTCTA CACTTTATAA AACCCAAGTA
    AAGGAGCTTA AAGAAGAAAT TGAAGAAAAA AACCGAGAAA ATTTAAGGAA AATACAGGAA
    CTGCAAAGTG AAAAAGAAAC TCTCTCTACT CAGTTGGATT TAGCAGAAAC AAAAGCTGAG
    TCTGAGCAGC TGGCGCGAGG GATTCTGGAA GAACAGTATT TTGAGTTGAC TCAGGAAAGC
    AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCATAC TGTTAGTCGG
    CTTGAAGAAA CAAACAGTGT GCTAACCAAA GATATTGAAA TGCTAAGAAA AGAAAATGAA
    GAGCTAAATG AAAGAATGAG GACAGCAGAA GAAGAATATA AATTGAAGAA GGAGGAAGAA
    ATAAATAATC TTAAGGCTGC TTTTGAAAAG AATATCAGTA CCGAACGGAC CCTTAAAACA
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGCAAAG ATTTTAAAAT TGATCGAAAG
    AAAGCTAATA CACAAGATTT AAGAAAAAAA GAAAAGGAAA ATAGAAAGCT ACAACTGGAA
    CTTAACCAAG AGAGAGAAAA ATTTAACCAA ATGGTGGTGA AACACCAGAA GGAGCTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACACATAGAA ATGAACTTCA AATGCAGTTG
    GCTAGCAAAG AGAGTGATAT TGAGCAGTTG CGGGCAAAAC TTTTGGACCT TTCGGATTCA
    ACTAGTGTTG CTAGTTTTCC TAGTGCAGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCTGT ACCAAATAGA GGAAATATCA AAAGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAGATC TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAGTC CATCTATGGT GTTAGACATA GATAAATTGT TTCATGTCCG ACCTGTAACC
    CAAGGAGATG TGTACAGAGC AGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATATA GAAGTGGAAC CGGTTCAACA AGGTGAAAAA
    ACTAATTTCC AGAATCACAA AGGCCATGAA TTCATTCCTA CCCTCTACCA CTTTCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTTTGTGG CATGTCTTTA AGCCACCACC TGCCCTAGAG
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GACCATCTAG ACAAGAAAGA GGACTTGATT
    TCCCCGTGCA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA GTGGGTTACT CATCTAGTAA AGAAGATTCC TAAGAACCCA
    CCATCTGGCT TTGTCCGTGC CTCTCCTCGA ACGCTGTCAA CAAGATCCAC TGCAAATCAG
    TCTTTCCGCA AAGTAGTCAA AAATACATCT GGAAAAACTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu54439
    Clone ID Related Accession (Same CDS sequence) XM_006525725.1 , XM_006525725.2
    Accession Version XM_006525725.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG
    GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG
    GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT
    TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC
    CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG
    CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT
    ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC
    TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA
    GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA
    GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT
    GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT
    AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG
    GATAAAAGCT TGGAAAGTTT ACAGAAAACA ATCTACAAGC TTGAAGAGCA ACTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACTT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAATCTAGA ATCTGCAGTG
    TCTCAAATTG AGAAAGAAAA GATGTTGCTA CAACATAGAA TTAATGAGTA CCAAAGAAAA
    GTGGAACAGG AAAATGAGAA GAGAAGAAAT ATAGAAAATG AAGTTTCTAC ACTAAAGGAC
    CAGTTAGAAG ACTTACGGAA AGCAAGTCAG ACCTCACAGC TTGCTAATGA GAAGCTGACA
    CAGTTACAGA AGCAGTTAGA AGAAGCGAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGTCA CACAGAGATG AGCAAGTCAA TTAGTCAGCT AGAGTCCCTG
    AACAGAGAAT TACAGGAAAG AAACAGAATT TTAGAGAATT CCAAGTCACA AGCAGACAAG
    GATTACTACC AGCTGCAAGC TGTATTGGAA GCTGAGCGAA GAGACAGAGG GCATGATTCT
    GAGATGATTG GAGACCTTCA AGCACGAATT ACATCTTTAC AAGAAGAAGT GAAACATCTC
    AAACATAATC TTGAAAGAGT GGAAGGAGAA AGAAAAGAGG CTCAAGACAT GCTTAATCAT
    TCAGAAAAGG AAAAGAATAA TTTGGAGATA GATTTGAATT ACAAGCTCAA GTCTATACAA
    CAGCGGTTAG AACAAGAAGT AAATGAACAT AAAGTCACCA AAGCACGCCT AACTGACAAG
    CACCAATCTA TCGAAGAGGC AAAGTCTGTT GCCATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCAGAA AATAGAGTTG TTGAGACTGA GAAACAATGT
    TCCATGCTAG ATGTTGACCT GAAACAGTCT CAGCAGAAGC TAGAACATTT GACCGAAAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTCGCCCTGC AGCTGGAACA GGAATCAAAT
    AAACGGCTCT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTTTAGAAA AACAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTGTTA
    GAATTTGAGT TAGCTCAGCT TACAAAGCAA TATAGGGGAA ATGAAGGGCA AATGCGGGAG
    TTACAAGATC AACTTGAAGC TGAGCAGTAT TTCTCTACAC TTTATAAAAC CCAAGTAAAG
    GAGCTTAAAG AAGAAATTGA AGAAAAAAAC CGAGAAAATT TAAGGAAAAT ACAGGAACTG
    CAAAGTGAAA AAGAAACTCT CTCTACTCAG TTGGATTTAG CAGAAACAAA AGCTGAGTCT
    GAGCAGCTGG CGCGAGGGAT TCTGGAAGAA CAGTATTTTG AGTTGACTCA GGAAAGCAAG
    AAAGCTGCTT CAAGAAATAG ACAAGAGATT ACAGATAAAG ATCATACTGT TAGTCGGCTT
    GAAGAAACAA ACAGTGTGCT AACCAAAGAT ATTGAAATGC TAAGAAAAGA AAATGAAGAG
    CTAAATGAAA GAATGAGGAC AGCAGAAGAA GAATATAAAT TGAAGAAGGA GGAAGAAATA
    AATAATCTTA AGGCTGCTTT TGAAAAGAAT ATCAGTACCG AACGGACCCT TAAAACACAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGCAAAGATT TTAAAATTGA TCGAAAGAAA
    GCTAATACAC AAGATTTAAG AAAAAAAGAA AAGGAAAATA GAAAGCTACA ACTGGAACTT
    AACCAAGAGA GAGAAAAATT TAACCAAATG GTGGTGAAAC ACCAGAAGGA GCTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACA CATAGAAATG AACTTCAAAT GCAGTTGGCT
    AGCAAAGAGA GTGATATTGA GCAGTTGCGG GCAAAACTTT TGGACCTTTC GGATTCAACT
    AGTGTTGCTA GTTTTCCTAG TGCAGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCTGTACC AAATAGAGGA AATATCAAAA GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAGATCTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAGTCCAT CTATGGTGTT AGACATAGAT AAATTGTTTC ATGTCCGACC TGTAACCCAA
    GGAGATGTGT ACAGAGCAGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATATAGAA GTGGAACCGG TTCAACAAGG TGAAAAAACT
    AATTTCCAGA ATCACAAAGG CCATGAATTC ATTCCTACCC TCTACCACTT TCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TTTGTGGCAT GTCTTTAAGC CACCACCTGC CCTAGAGTGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAC CATCTAGACA AGAAAGAGGA CTTGATTTCC
    CCGTGCAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAGTG GGTTACTCAT CTAGTAAAGA AGATTCCTAA GAACCCACCA
    TCTGGCTTTG TCCGTGCCTC TCCTCGAACG CTGTCAACAA GATCCACTGC AAATCAGTCT
    TTCCGCAAAG TAGTCAAAAA TACATCTGGA AAAACTAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006525788.1
    CDS476..4537
    Translation

    Target ORF information:

    RefSeq Version XM_006525725.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006525725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG
    GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG
    GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT
    TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC
    CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG
    CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT
    ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC
    TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA
    GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA
    GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT
    GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT
    AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG
    GATAAAAGCT TGGAAAGTTT ACAGAAAACA ATCTACAAGC TTGAAGAGCA ACTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACTT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAATCTAGA ATCTGCAGTG
    TCTCAAATTG AGAAAGAAAA GATGTTGCTA CAACATAGAA TTAATGAGTA CCAAAGAAAA
    GTGGAACAGG AAAATGAGAA GAGAAGAAAT ATAGAAAATG AAGTTTCTAC ACTAAAGGAC
    CAGTTAGAAG ACTTACGGAA AGCAAGTCAG ACCTCACAGC TTGCTAATGA GAAGCTGACA
    CAGTTACAGA AGCAGTTAGA AGAAGCGAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGTCA CACAGAGATG AGCAAGTCAA TTAGTCAGCT AGAGTCCCTG
    AACAGAGAAT TACAGGAAAG AAACAGAATT TTAGAGAATT CCAAGTCACA AGCAGACAAG
    GATTACTACC AGCTGCAAGC TGTATTGGAA GCTGAGCGAA GAGACAGAGG GCATGATTCT
    GAGATGATTG GAGACCTTCA AGCACGAATT ACATCTTTAC AAGAAGAAGT GAAACATCTC
    AAACATAATC TTGAAAGAGT GGAAGGAGAA AGAAAAGAGG CTCAAGACAT GCTTAATCAT
    TCAGAAAAGG AAAAGAATAA TTTGGAGATA GATTTGAATT ACAAGCTCAA GTCTATACAA
    CAGCGGTTAG AACAAGAAGT AAATGAACAT AAAGTCACCA AAGCACGCCT AACTGACAAG
    CACCAATCTA TCGAAGAGGC AAAGTCTGTT GCCATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCAGAA AATAGAGTTG TTGAGACTGA GAAACAATGT
    TCCATGCTAG ATGTTGACCT GAAACAGTCT CAGCAGAAGC TAGAACATTT GACCGAAAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTCGCCCTGC AGCTGGAACA GGAATCAAAT
    AAACGGCTCT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTTTAGAAA AACAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTGTTA
    GAATTTGAGT TAGCTCAGCT TACAAAGCAA TATAGGGGAA ATGAAGGGCA AATGCGGGAG
    TTACAAGATC AACTTGAAGC TGAGCAGTAT TTCTCTACAC TTTATAAAAC CCAAGTAAAG
    GAGCTTAAAG AAGAAATTGA AGAAAAAAAC CGAGAAAATT TAAGGAAAAT ACAGGAACTG
    CAAAGTGAAA AAGAAACTCT CTCTACTCAG TTGGATTTAG CAGAAACAAA AGCTGAGTCT
    GAGCAGCTGG CGCGAGGGAT TCTGGAAGAA CAGTATTTTG AGTTGACTCA GGAAAGCAAG
    AAAGCTGCTT CAAGAAATAG ACAAGAGATT ACAGATAAAG ATCATACTGT TAGTCGGCTT
    GAAGAAACAA ACAGTGTGCT AACCAAAGAT ATTGAAATGC TAAGAAAAGA AAATGAAGAG
    CTAAATGAAA GAATGAGGAC AGCAGAAGAA GAATATAAAT TGAAGAAGGA GGAAGAAATA
    AATAATCTTA AGGCTGCTTT TGAAAAGAAT ATCAGTACCG AACGGACCCT TAAAACACAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGCAAAGATT TTAAAATTGA TCGAAAGAAA
    GCTAATACAC AAGATTTAAG AAAAAAAGAA AAGGAAAATA GAAAGCTACA ACTGGAACTT
    AACCAAGAGA GAGAAAAATT TAACCAAATG GTGGTGAAAC ACCAGAAGGA GCTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACA CATAGAAATG AACTTCAAAT GCAGTTGGCT
    AGCAAAGAGA GTGATATTGA GCAGTTGCGG GCAAAACTTT TGGACCTTTC GGATTCAACT
    AGTGTTGCTA GTTTTCCTAG TGCAGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCTGTACC AAATAGAGGA AATATCAAAA GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAGATCTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAGTCCAT CTATGGTGTT AGACATAGAT AAATTGTTTC ATGTCCGACC TGTAACCCAA
    GGAGATGTGT ACAGAGCAGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATATAGAA GTGGAACCGG TTCAACAAGG TGAAAAAACT
    AATTTCCAGA ATCACAAAGG CCATGAATTC ATTCCTACCC TCTACCACTT TCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TTTGTGGCAT GTCTTTAAGC CACCACCTGC CCTAGAGTGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAC CATCTAGACA AGAAAGAGGA CTTGATTTCC
    CCGTGCAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAGTG GGTTACTCAT CTAGTAAAGA AGATTCCTAA GAACCCACCA
    TCTGGCTTTG TCCGTGCCTC TCCTCGAACG CTGTCAACAA GATCCACTGC AAATCAGTCT
    TTCCGCAAAG TAGTCAAAAA TACATCTGGA AAAACTAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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