Slc10a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Slc10a2
Entrez Gene ID	20494
Full Name	solute carrier family 10, member 2
Synonyms	9130221J18Rik,AI605518,ASBT,IBAT,ISBT
General protein information	Preferred Names solute carrier family 10, member 2 Names ileal sodium/bile acid cotransporter Na(+)-dependent ileal bile acid transporter apical sodium-dependent bile acid transporter ileal Na(+)/bile acid cotransporter ileal sodium-dependent bile acid transporter sodium/taurocholate cotransporting polypeptide, ileal
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 2.16 cM

mRNA and Protein(s)

mRNA Protein Name

NM_011388.2 NP_035518.1 ileal sodium/bile acid cotransporter

NM_011388.3 NP_035518.1 ileal sodium/bile acid cotransporter
Pathways from BioSystems

mRNA	Protein	Name
NM_011388.2	NP_035518.1	ileal sodium/bile acid cotransporter
NM_011388.3	NP_035518.1	ileal sodium/bile acid cotransporter

Homologies

Gallus gallus (chicken)	SLC10A2	XP_425589.3
Danio rerio (zebrafish)	slc10a2	NP_956652.1
Drosophila melanogaster (fruit fly)	CG9903	NP_573132.1
Xenopus tropicalis (tropical clawed frog)	LOC100487141	XP_002933069.1
Homo sapiens (human)	SLC10A2	NP_000443.1
Mus musculus (house mouse)	Slc10a2	NP_035518.1
Arabidopsis thaliana (thale cress)	AT2G26900	NP_850089.1
Canis lupus familiaris (dog)	SLC10A2	NP_001002968.1
Rattus norvegicus (Norway rat)	Slc10a2	NP_058918.1
Pan troglodytes (chimpanzee)	SLC10A2	XP_522716.2
	SLC10A2	XP_001095212.1
Bos taurus (cattle)	SLC10A2	XP_002692028.2
Oryza sativa (rice)	Os01g0645200	NP_001043701.1

Gene Ontology
Bibliography

Related articles in PubMed

The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids.
Cao W, Kayama H, Chen ML, Delmas A, Sun A, Kim SY, Rangarajan ES, McKevitt K, Beck AP, Jackson CB, Crynen G, Oikonomopoulos A, Lacey PN, Martinez GJ, Izard T, Lorenz RG, Rodriguez-Palacios A, Cominelli F, Abreu MT, Hommes DW, Koralov SB, Takeda K, Sundrud MS
Immunity47(6)1182-1196.e10(2017 Dec)

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Ferrell JM, Boehme S, Li F, Chiang JY
Journal of lipid research57(7)1144-54(2016 07)

Slc10a2-null mice uncover colon cancer-promoting actions of endogenous fecal bile acids.
Raufman JP, Dawson PA, Rao A, Drachenberg CB, Heath J, Shang AC, Hu S, Zhan M, Polli JE, Cheng K
Carcinogenesis36(10)1193-200(2015 Oct)

Gut microbiota inhibit Asbt-dependent intestinal bile acid reabsorption via Gata4.
Out C, Patankar JV, Doktorova M, Boesjes M, Bos T, de Boer S, Havinga R, Wolters H, Boverhof R, van Dijk TH, Smoczek A, Bleich A, Sachdev V, Kratky D, Kuipers F, Verkade HJ, Groen AK
Journal of hepatology63(3)697-704(2015 Sep)

Maternal bile acid transporter deficiency promotes neonatal demise.
Zhang Y, Li F, Wang Y, Pitre A, Fang ZZ, Frank MW, Calabrese C, Krausz KW, Neale G, Frase S, Vogel P, Rock CO, Gonzalez FJ, Schuetz JD
Nature communications68186(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Asbt-deficient mice, the lack of bile acid uptake in the distal small intestine, which we recently documented using novel in vivo imaging results in more than 10-fold increased fecal bile acids
Title: Slc10a2-null mice uncover colon cancer-promoting actions of endogenous fecal bile acids.

data support the concept that under physiological conditions microbiota stimulate Gata4, which suppresses Asbt expression
Title: Gut microbiota inhibit Asbt-dependent intestinal bile acid reabsorption via Gata4.

ileal ASBT protein is degraded by a ubiquitin-dependent pathway in response to enterobacteria-associated bile acids
Title: Ileal apical sodium-dependent bile acid transporter protein levels are down-regulated through ubiquitin-dependent protein degradation induced by bile acids.

Ileal Fibroblast Growth Factor-15 (FGF15) expression was significantly reduced in Asbt(-/-)apoE(-/-) mice and was inversely correlated with expression of hepatic cholesterol 7-hydroxylase (Cyp7a1).
Title: Inhibition of ileal apical but not basolateral bile acid transport reduces atherosclerosis in apoE?/? mice.

The functional importance of proline and uncharged polar residues in the highly conserved region of mouse Slc10a2 was determined.
Title: Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport activity and cellular expression.

The following Slc10a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc10a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20357	NM_011388.3 Latest version!	Mus musculus solute carrier family 10, member 2 (Slc10a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OMu20357	NM_011388.2	Mus musculus solute carrier family 10, member 2 (Slc10a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc10a2 ( NM_011388.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035518.1 Mus musculus solute carrier family 10, member 2 (Slc10a2), mRNA.

Price & Availability↑

CloneID	OMu20357
Clone ID Related Accession (Same CDS sequence)	NM_011388.2 , NM_011388.3
Accession Version	NM_011388.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	ileal sodium/bile acid cotransporter
Comment

ATGGATAACT CCTCTGTCTG TCCCCCAAAT GCAACTGTCT GCGAAGGCGA TTCCTGCGTA 
GTACCTGAGA GCAACTTCAA TGCAATTCTC AATACAGTGA TGAGCACTGT GCTCACCATC 
CTCTTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAGTCCACAA GTTCCTAGGA 
CATATAAAGA GACCATGGGG TATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGCT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTACAGGC TGTAGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGCGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC CCTTGGAATG 
ATGCCTCTTT GCCTCTTCGT CTACACCAAG ATGTGGGTTG ACTCGGGAAC GATTGTGATT 
CCCTATGATA GCATTGGTAT TTCTCTGGTT GCTCTTGTTA TTCCTGTTTC CTTTGGAATG 
TTTGTAAATC ACAAATGGCC ACAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCACA 
GGTGTAATTC TCATTGTGCT CATAGCTGTG ATTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTGTG GATTATAGGA ACAATATTCC CTATAGCTGG CTACAGCCTG 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTAGCCTTG 
GAAACTGGAA TGCAGAACAC TCAGCTGTGC TCCACCATTG TACAGCTCTC CTTCTCCCCC 
GAGGATCTCA ACCTGGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAGTAA TATTAGGAAT TTATGTCACA TACAGGAAAT GTTATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGAAATG GACTCCAGGC CATCATTTGA TGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035518.1
Translation	MDNSSVCPPNATVCEGDSCVVPESNFNAILNTVMSTVLTILLAMVMFSMGCNVEVHKFLGHIKRPWGIFVGFLCQFGIMPLTGFILSVASGILPVQAVVVLIMGCCPGGTGSNILAYWIDGDMDLSVSMTTCSTLLALGMMPLCLFVYTKMWVDSGTIVIPYDSIGISLVALVIPVSFGMFVNHKWPQKAKIILKIGSITGVILIVLIAVIGGILYQSAWIIEPKLWIIGTIFPIAGYSLGFFLARLAGQPWYRCRTVALETGMQNTQLCSTIVQLSFSPEDLNLVFTFPLIYTVFQLVFAAVILGIYVTYRKCYGKNDAEFLEKTDNEMDSRPSFDETNKGFQPDEK

Target ORF information:

RefSeq Version	NM_011388.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus solute carrier family 10, member 2 (Slc10a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011388.2

ORF Insert Sequence:

ATGGATAACT CCTCTGTCTG TCCCCCAAAT GCAACTGTCT GCGAAGGCGA TTCCTGCGTA 
GTACCTGAGA GCAACTTCAA TGCAATTCTC AATACAGTGA TGAGCACTGT GCTCACCATC 
CTCTTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAGTCCACAA GTTCCTAGGA 
CATATAAAGA GACCATGGGG TATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGCT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTACAGGC TGTAGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGCGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC CCTTGGAATG 
ATGCCTCTTT GCCTCTTCGT CTACACCAAG ATGTGGGTTG ACTCGGGAAC GATTGTGATT 
CCCTATGATA GCATTGGTAT TTCTCTGGTT GCTCTTGTTA TTCCTGTTTC CTTTGGAATG 
TTTGTAAATC ACAAATGGCC ACAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCACA 
GGTGTAATTC TCATTGTGCT CATAGCTGTG ATTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTGTG GATTATAGGA ACAATATTCC CTATAGCTGG CTACAGCCTG 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTAGCCTTG 
GAAACTGGAA TGCAGAACAC TCAGCTGTGC TCCACCATTG TACAGCTCTC CTTCTCCCCC 
GAGGATCTCA ACCTGGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAGTAA TATTAGGAAT TTATGTCACA TACAGGAAAT GTTATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGAAATG GACTCCAGGC CATCATTTGA TGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc10a2 ( NM_011388.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035518.1 Mus musculus solute carrier family 10, member 2 (Slc10a2), mRNA.

Price & Availability↑

CloneID	OMu20357
Clone ID Related Accession (Same CDS sequence)	NM_011388.2 , NM_011388.3
Accession Version	NM_011388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	ileal sodium/bile acid cotransporter
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110744.6. On May 24, 2016 this sequence version replaced NM_011388.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002693.1, AY529655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGATAACT CCTCTGTCTG TCCCCCAAAT GCAACTGTCT GCGAAGGCGA TTCCTGCGTA 
GTACCTGAGA GCAACTTCAA TGCAATTCTC AATACAGTGA TGAGCACTGT GCTCACCATC 
CTCTTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAGTCCACAA GTTCCTAGGA 
CATATAAAGA GACCATGGGG TATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGCT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTACAGGC TGTAGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGCGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC CCTTGGAATG 
ATGCCTCTTT GCCTCTTCGT CTACACCAAG ATGTGGGTTG ACTCGGGAAC GATTGTGATT 
CCCTATGATA GCATTGGTAT TTCTCTGGTT GCTCTTGTTA TTCCTGTTTC CTTTGGAATG 
TTTGTAAATC ACAAATGGCC ACAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCACA 
GGTGTAATTC TCATTGTGCT CATAGCTGTG ATTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTGTG GATTATAGGA ACAATATTCC CTATAGCTGG CTACAGCCTG 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTAGCCTTG 
GAAACTGGAA TGCAGAACAC TCAGCTGTGC TCCACCATTG TACAGCTCTC CTTCTCCCCC 
GAGGATCTCA ACCTGGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAGTAA TATTAGGAAT TTATGTCACA TACAGGAAAT GTTATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGAAATG GACTCCAGGC CATCATTTGA TGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035518.1
CDS	105..1151
Translation	MDNSSVCPPNATVCEGDSCVVPESNFNAILNTVMSTVLTILLAMVMFSMGCNVEVHKFLGHIKRPWGIFVGFLCQFGIMPLTGFILSVASGILPVQAVVVLIMGCCPGGTGSNILAYWIDGDMDLSVSMTTCSTLLALGMMPLCLFVYTKMWVDSGTIVIPYDSIGISLVALVIPVSFGMFVNHKWPQKAKIILKIGSITGVILIVLIAVIGGILYQSAWIIEPKLWIIGTIFPIAGYSLGFFLARLAGQPWYRCRTVALETGMQNTQLCSTIVQLSFSPEDLNLVFTFPLIYTVFQLVFAAVILGIYVTYRKCYGKNDAEFLEKTDNEMDSRPSFDETNKGFQPDEK

Target ORF information:

RefSeq Version	NM_011388.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 10, member 2 (Slc10a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011388.3

ORF Insert Sequence:

ATGGATAACT CCTCTGTCTG TCCCCCAAAT GCAACTGTCT GCGAAGGCGA TTCCTGCGTA 
GTACCTGAGA GCAACTTCAA TGCAATTCTC AATACAGTGA TGAGCACTGT GCTCACCATC 
CTCTTAGCCA TGGTGATGTT TTCTATGGGG TGCAATGTGG AAGTCCACAA GTTCCTAGGA 
CATATAAAGA GACCATGGGG TATCTTCGTG GGCTTCCTCT GTCAGTTTGG AATCATGCCT 
CTCACAGGCT TTATCCTGTC TGTGGCCTCT GGCATCCTTC CTGTACAGGC TGTAGTGGTG 
CTAATTATGG GTTGCTGCCC TGGAGGAACT GGCTCCAATA TCCTGGCCTA TTGGATAGAT 
GGCGACATGG ACCTCAGTGT TAGCATGACC ACTTGCTCCA CACTGCTTGC CCTTGGAATG 
ATGCCTCTTT GCCTCTTCGT CTACACCAAG ATGTGGGTTG ACTCGGGAAC GATTGTGATT 
CCCTATGATA GCATTGGTAT TTCTCTGGTT GCTCTTGTTA TTCCTGTTTC CTTTGGAATG 
TTTGTAAATC ACAAATGGCC ACAAAAAGCG AAGATTATAC TTAAAATTGG ATCCATCACA 
GGTGTAATTC TCATTGTGCT CATAGCTGTG ATTGGAGGAA TACTGTACCA AAGTGCCTGG 
ATCATTGAAC CCAAACTGTG GATTATAGGA ACAATATTCC CTATAGCTGG CTACAGCCTG 
GGTTTCTTCC TGGCTAGACT AGCTGGTCAA CCCTGGTACA GGTGCCGAAC AGTAGCCTTG 
GAAACTGGAA TGCAGAACAC TCAGCTGTGC TCCACCATTG TACAGCTCTC CTTCTCCCCC 
GAGGATCTCA ACCTGGTGTT CACCTTCCCA CTCATCTATA CTGTTTTCCA GCTCGTCTTT 
GCAGCAGTAA TATTAGGAAT TTATGTCACA TACAGGAAAT GTTATGGAAA AAATGATGCT 
GAGTTTCTAG AGAAAACAGA CAATGAAATG GACTCCAGGC CATCATTTGA TGAGACAAAC 
AAGGGATTTC AACCAGATGA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids.
Immunity47(6)1182-1196.e10(2017 Dec)
Cao W,Kayama H,Chen ML,Delmas A,Sun A,Kim SY,Rangarajan ES,McKevitt K,Beck AP,Jackson CB,Crynen G,Oikonomopoulos A,Lacey PN,Martinez GJ,Izard T,Lorenz RG,Rodriguez-Palacios A,Cominelli F,Abreu MT,Hommes DW,Koralov SB,Takeda K,Sundrud MS

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Journal of lipid research57(7)1144-54(2016 07)
Ferrell JM,Boehme S,Li F,Chiang JY

Slc10a2-null mice uncover colon cancer-promoting actions of endogenous fecal bile acids.
Carcinogenesis36(10)1193-200(2015 Oct)
Raufman JP,Dawson PA,Rao A,Drachenberg CB,Heath J,Shang AC,Hu S,Zhan M,Polli JE,Cheng K

Gut microbiota inhibit Asbt-dependent intestinal bile acid reabsorption via Gata4.
Journal of hepatology63(3)697-704(2015 Sep)
Out C,Patankar JV,Doktorova M,Boesjes M,Bos T,de Boer S,Havinga R,Wolters H,Boverhof R,van Dijk TH,Smoczek A,Bleich A,Sachdev V,Kratky D,Kuipers F,Verkade HJ,Groen AK

Maternal bile acid transporter deficiency promotes neonatal demise.
Nature communications68186(2015 Sep)
Zhang Y,Li F,Wang Y,Pitre A,Fang ZZ,Frank MW,Calabrese C,Krausz KW,Neale G,Frase S,Vogel P,Rock CO,Gonzalez FJ,Schuetz JD

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Slc10a2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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