Themis2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Themis2
Entrez Gene ID	230787
Full Name	thymocyte selection associated family member 2
Synonyms	ICB-1
General protein information	Preferred Names thymocyte selection associated family member 2 Names protein THEMIS2 basement membrane-induced protein induced by contact to basement membrane 1 protein protein ICB-1 thymocyte-expressed molecule involved in selection protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.3

mRNA and Protein(s)

mRNA Protein Name

XM_011250230.3 XP_011248532.1 protein THEMIS2 isoform X1

XM_011250230.2 XP_011248532.1 protein THEMIS2 isoform X1

NM_001033308.2 NP_001028480.1 protein THEMIS2
Pathways from BioSystems

mRNA	Protein	Name
XM_011250230.3	XP_011248532.1	protein THEMIS2 isoform X1
XM_011250230.2	XP_011248532.1	protein THEMIS2 isoform X1
NM_001033308.2	NP_001028480.1	protein THEMIS2

Homologies

Canis lupus familiaris (dog)	THEMIS2	XP_003638987.1
Mus musculus (house mouse)	Themis2	NP_001028480.1
Danio rerio (zebrafish)	LOC100535600	XP_005157914.1
	THEMIS2	XP_001112611.1
Bos taurus (cattle)	THEMIS2	XP_002685734.1
Homo sapiens (human)	THEMIS2	NP_001099026.1
Xenopus tropicalis (tropical clawed frog)	themis2	XP_004912375.1
Pan troglodytes (chimpanzee)	THEMIS2	XP_003307966.1
Rattus norvegicus (Norway rat)	Themis2	NP_001258409.1
Gallus gallus (chicken)	C23H1ORF38	XP_417712.2

Gene Ontology
Bibliography

Related articles in PubMed

Themis2 lowers the threshold for B cell activation during positive selection.
Cheng D, Deobagkar-Lele M, Zvezdova E, Choi S, Uehara S, Baup D, Bennett SC, Bull KR, Crockford TL, Ferry H, Warzecha C, Marcellin M, de Peredo AG, Lesourne R, Anzilotti C, Love PE, Cornall RJ
Nature immunology18(2)205-213(2017 Feb)

Themis2 is not required for B cell development, activation, and antibody responses.
Hartweger H, Schweighoffer E, Davidson S, Peirce MJ, Wack A, Tybulewicz VL
Journal of immunology (Baltimore, Md. : 1950)193(2)700-7(2014 Jul)

Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.
Lesourne R, Zvezdova E, Song KD, El-Khoury D, Uehara S, Barr VA, Samelson LE, Love PE
Journal of immunology (Baltimore, Md. : 1950)189(3)1154-61(2012 Aug)

Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production.
Peirce MJ, Brook M, Morrice N, Snelgrove R, Begum S, Lanfrancotti A, Notley C, Hussell T, Cope AP, Wait R
PloS one5(7)e11465(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this stuidy identifies a unique function for Themis2 in differential signaling and provide insight into how B cells discriminate between antigens of different quantity and quality
Title: Themis2 lowers the threshold for B cell activation during positive selection.

Data indicate that Themis2 protein is not required for B cell development, for activation, or for antibody responses either to model Ags or to influenza viral infection.
Title: Themis2 is not required for B cell development, activation, and antibody responses.

Study hypothesise that Themis2 may constitute a novel, physiological control point in macrophage inflammatory responses.
Title: Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production.

The following Themis2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Themis2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17965	NM_001033308.2 Latest version!	Mus musculus thymocyte selection associated family member 2 (Themis2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$391.30 $559.00
OMu73533	XM_011250230.3 Latest version!	Mus musculus thymocyte selection associated family member 2 (Themis2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu73533	XM_011250230.2	Mus musculus thymocyte selection associated family member 2 (Themis2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Themis2 ( NM_001033308.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028480.1 Mus musculus thymocyte selection associated family member 2 (Themis2), mRNA.

Price & Availability↑

CloneID	OMu17965
Clone ID Related Accession (Same CDS sequence)	NM_001033308.2
Accession Version	NM_001033308.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	protein THEMIS2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK150471.1. On Apr 6, 2007 this sequence version replaced NM_001033308.1. ##Evidence-Data-START## Transcript exon combination :: AK150471.1, AK153536.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCGG TGCCGCTGCA GGACTTTGTG AGCGGCCTGG ACCCCACCTC CCTTCCGCGC 
GTGCTGCGGG TCTGCTCCGG AGTCTACTTT GAGGGTTCCG TCTACGAGCT CTTTGGGAAT 
GAGTGCTGCC TCTCCACCGG GGACCTAATC AAGGTCACTC ATGTCCAGCT CCAAAAGGTT 
GTGTGCGAGT ATCCAGAGAC GGGTCAGACG TTGGAGCTCA ACCCCAATTT CACAGGCCTC 
TTCAGCCCAC TCACCAGCCT GCGAAGCTAC AGAACCCTGG AGGACCTTGT CTCTGCCATG 
CCCCAGAACT CCACCCGGTG GCCCATTTAC TTCAAGTCAA CTCAGAGAAT TGTCACCAAG 
GCCAGTGTGG TGCCTGAGGA TCAGCCCCTC AGACTTGAGG CTGTGGAAAT ACACCATGGG 
ATCCGCTATG CGCGCTGCGT CCAGGTCTCA AAGACCAAGG AGCTCCTTCA TCTGCCCCTT 
TCCCAGAAGG GACCCTTCTG GAGATGCAAG CCCAGTGCTC CACAAACTCT GCACCAAATC 
CTGCAGGACC CAGCTCTGAA GGACCTCACG CTCAGCTGCC CCAGCCTACC CTGGAACTCC 
GTGATCCTAA AACCCCAGTA TATGCTCCAA GCCATCATGC ACATGCGCAG CAGCATCGTC 
AAGATCCCGT CTACGCTGGA GGTGGAGGTA GAAGACGTCA CTGCTTCCTC CCAACACATC 
CACTTCTTCA AACCCTTGCG GCTGAGCGAG GTGTTGGCTG GGGGAGGCCC CTTCCCTCTG 
ACCACGGAGA TCCTGGAGGT TCCTGAGGGT CCGCCCGTCT TCCTCAGCCC GTGGGTGAGC 
TTCCTACGCA AAGGCCAGAG GCTCTGCATC TATGGTCCAG CTTCACCATC CTGGCGGGTT 
GTGGCCTCCA GCAAGAGCCG AAAAGTGCCC AGATACTTCA TGCTCTCTGG GGCCTACCAG 
GGCAAGCTCA AGCGGAGGCC CAGGGAGTTC TCCACCGCCT ATGACCTTTT GGGGGCTCTC 
CAGCCAGGCC GGCCCCTCCG GGTGGTAGCC ACAAAAGACT GTGATGGGAA TGAGGAAGAG 
AATCCTGACT TTTCTTTCTT GGCTGTGGGT GATCGGCTGG AAGTGCTGAG GTCAGGCCAG 
GTCTGTGGGA CCAAAGGCCA GGACATAGAT GTCCTGGTAT GTCAGCGGCT GAGTGAGCAG 
TCTGGGGAGG AAGAGGAGGA CTTGGAGGAG ATCGAGGACG AGGCAGAGGA CAAAGAGCAA 
ATTCTGCTAC CCCTCTACCT CTCTGGGAGC TTTGTGGAGG AGGTGAACGA CAGCCGACGC 
TATAACCTAG TGGATCTCAC GGCCCAGTAC TCCCTGCCCT GTGAGGTCAA GGTGGTGACC 
AAGGATACCC GTCACCCCAC TGACCCCCTG GCTTCCTTCC CTGGCCTGCG GCTGGAGGAG 
AAGCTCACAG AACCCTTTTT GGTGGTAAGC CTGGACTCCC AGCCAGAAAT GTGCTTTGAG 
ATCCCTCCCC GGTGGCTGGA CCTGACTGTG GTGGAGGCTG AGGGACAGCC AGCCCAGGTG 
GCTCGGCCGC TGTCCATCGC TCCGGTGGAG GAACTGTCAG AAGCTTTCTA CTACAGCCTC 
CGGAAGCTGC CAGCCTCTGA GAGCCAAGCC CCCCCGCCCA GGCCCCCAAA GAGCCAGGGT 
ATCAATAAGA AACAGCAGAA CATACAGAGC TGTAAAGAGA GCAGTGTAAA GCCTCAAGTT 
GTAGAACCGC AGAAATCCTG CCCGCAGCCT CAACTCAAGG CGAAGACCTT GGAAGCCCTT 
CCCAAGAACA AATCAAATGT GTACAGCAAG ATTTCTGTCC ACAAGAAAGA CCGCAAACCC 
AACCCCCAAA CACAGAATTC AGTGCTGAGT ATGAAGCCCA AAACCTCGAG CTCCCTCGGC 
AAGCACTCTA CCATGGAGTC ACATCTCCTG CCTGACCCGG ATATGGATGA CCATGACTAT 
GAAGAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028480.1
CDS	12..2003
Translation	MEPVPLQDFVSGLDPTSLPRVLRVCSGVYFEGSVYELFGNECCLSTGDLIKVTHVQLQKVVCEYPETGQTLELNPNFTGLFSPLTSLRSYRTLEDLVSAMPQNSTRWPIYFKSTQRIVTKASVVPEDQPLRLEAVEIHHGIRYARCVQVSKTKELLHLPLSQKGPFWRCKPSAPQTLHQILQDPALKDLTLSCPSLPWNSVILKPQYMLQAIMHMRSSIVKIPSTLEVEVEDVTASSQHIHFFKPLRLSEVLAGGGPFPLTTEILEVPEGPPVFLSPWVSFLRKGQRLCIYGPASPSWRVVASSKSRKVPRYFMLSGAYQGKLKRRPREFSTAYDLLGALQPGRPLRVVATKDCDGNEEENPDFSFLAVGDRLEVLRSGQVCGTKGQDIDVLVCQRLSEQSGEEEEDLEEIEDEAEDKEQILLPLYLSGSFVEEVNDSRRYNLVDLTAQYSLPCEVKVVTKDTRHPTDPLASFPGLRLEEKLTEPFLVVSLDSQPEMCFEIPPRWLDLTVVEAEGQPAQVARPLSIAPVEELSEAFYYSLRKLPASESQAPPPRPPKSQGINKKQQNIQSCKESSVKPQVVEPQKSCPQPQLKAKTLEALPKNKSNVYSKISVHKKDRKPNPQTQNSVLSMKPKTSSSLGKHSTMESHLLPDPDMDDHDYEEI

Target ORF information:

RefSeq Version	NM_001033308.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated family member 2 (Themis2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033308.2

ORF Insert Sequence:

ATGGAGCCGG TGCCGCTGCA GGACTTTGTG AGCGGCCTGG ACCCCACCTC CCTTCCGCGC 
GTGCTGCGGG TCTGCTCCGG AGTCTACTTT GAGGGTTCCG TCTACGAGCT CTTTGGGAAT 
GAGTGCTGCC TCTCCACCGG GGACCTAATC AAGGTCACTC ATGTCCAGCT CCAAAAGGTT 
GTGTGCGAGT ATCCAGAGAC GGGTCAGACG TTGGAGCTCA ACCCCAATTT CACAGGCCTC 
TTCAGCCCAC TCACCAGCCT GCGAAGCTAC AGAACCCTGG AGGACCTTGT CTCTGCCATG 
CCCCAGAACT CCACCCGGTG GCCCATTTAC TTCAAGTCAA CTCAGAGAAT TGTCACCAAG 
GCCAGTGTGG TGCCTGAGGA TCAGCCCCTC AGACTTGAGG CTGTGGAAAT ACACCATGGG 
ATCCGCTATG CGCGCTGCGT CCAGGTCTCA AAGACCAAGG AGCTCCTTCA TCTGCCCCTT 
TCCCAGAAGG GACCCTTCTG GAGATGCAAG CCCAGTGCTC CACAAACTCT GCACCAAATC 
CTGCAGGACC CAGCTCTGAA GGACCTCACG CTCAGCTGCC CCAGCCTACC CTGGAACTCC 
GTGATCCTAA AACCCCAGTA TATGCTCCAA GCCATCATGC ACATGCGCAG CAGCATCGTC 
AAGATCCCGT CTACGCTGGA GGTGGAGGTA GAAGACGTCA CTGCTTCCTC CCAACACATC 
CACTTCTTCA AACCCTTGCG GCTGAGCGAG GTGTTGGCTG GGGGAGGCCC CTTCCCTCTG 
ACCACGGAGA TCCTGGAGGT TCCTGAGGGT CCGCCCGTCT TCCTCAGCCC GTGGGTGAGC 
TTCCTACGCA AAGGCCAGAG GCTCTGCATC TATGGTCCAG CTTCACCATC CTGGCGGGTT 
GTGGCCTCCA GCAAGAGCCG AAAAGTGCCC AGATACTTCA TGCTCTCTGG GGCCTACCAG 
GGCAAGCTCA AGCGGAGGCC CAGGGAGTTC TCCACCGCCT ATGACCTTTT GGGGGCTCTC 
CAGCCAGGCC GGCCCCTCCG GGTGGTAGCC ACAAAAGACT GTGATGGGAA TGAGGAAGAG 
AATCCTGACT TTTCTTTCTT GGCTGTGGGT GATCGGCTGG AAGTGCTGAG GTCAGGCCAG 
GTCTGTGGGA CCAAAGGCCA GGACATAGAT GTCCTGGTAT GTCAGCGGCT GAGTGAGCAG 
TCTGGGGAGG AAGAGGAGGA CTTGGAGGAG ATCGAGGACG AGGCAGAGGA CAAAGAGCAA 
ATTCTGCTAC CCCTCTACCT CTCTGGGAGC TTTGTGGAGG AGGTGAACGA CAGCCGACGC 
TATAACCTAG TGGATCTCAC GGCCCAGTAC TCCCTGCCCT GTGAGGTCAA GGTGGTGACC 
AAGGATACCC GTCACCCCAC TGACCCCCTG GCTTCCTTCC CTGGCCTGCG GCTGGAGGAG 
AAGCTCACAG AACCCTTTTT GGTGGTAAGC CTGGACTCCC AGCCAGAAAT GTGCTTTGAG 
ATCCCTCCCC GGTGGCTGGA CCTGACTGTG GTGGAGGCTG AGGGACAGCC AGCCCAGGTG 
GCTCGGCCGC TGTCCATCGC TCCGGTGGAG GAACTGTCAG AAGCTTTCTA CTACAGCCTC 
CGGAAGCTGC CAGCCTCTGA GAGCCAAGCC CCCCCGCCCA GGCCCCCAAA GAGCCAGGGT 
ATCAATAAGA AACAGCAGAA CATACAGAGC TGTAAAGAGA GCAGTGTAAA GCCTCAAGTT 
GTAGAACCGC AGAAATCCTG CCCGCAGCCT CAACTCAAGG CGAAGACCTT GGAAGCCCTT 
CCCAAGAACA AATCAAATGT GTACAGCAAG ATTTCTGTCC ACAAGAAAGA CCGCAAACCC 
AACCCCCAAA CACAGAATTC AGTGCTGAGT ATGAAGCCCA AAACCTCGAG CTCCCTCGGC 
AAGCACTCTA CCATGGAGTC ACATCTCCTG CCTGACCCGG ATATGGATGA CCATGACTAT 
GAAGAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Themis2 ( XM_011250230.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248532.1 Mus musculus thymocyte selection associated family member 2 (Themis2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73533
Clone ID Related Accession (Same CDS sequence)	XM_011250230.3 , XM_011250230.2
Accession Version	XM_011250230.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein THEMIS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA ACTCCACCCG GTGGCCCATT TACTTCAAGT CAACTCAGAG AATTGTCACC 
AAGGCCAGTG TGGTGCCTGA GGATCAGCCC CTCAGACTTG AGGCTGTGGA AATACACCAT 
GGGATCCGCT ATGCGCGCTG CGTCCAGGTC TCAAAGACCA AGGAGCTCCT TCATCTGCCC 
CTTTCCCAGA AGGGACCCTT CTGGAGATGC AAGCCCAGTG CTCCACAAAC TCTGCACCAA 
ATCCTGCAGG ACCCAGCTCT GAAGGACCTC ACGCTCAGCT GCCCCAGCCT ACCCTGGAAC 
TCCGTGATCC TAAAACCCCA GTATATGCTC CAAGCCATCA TGCACATGCG CAGCAGCATC 
GTCAAGATCC CGTCTACGCT GGAGGTGGAG GTAGAAGACG TCACTGCTTC CTCCCAACAC 
ATCCACTTCT TCAAACCCTT GCGGCTGAGC GAGGTGTTGG CTGGGGGAGG CCCCTTCCCT 
CTGACCACGG AGATCCTGGA GGTTCCTGAG GGTCCGCCCG TCTTCCTCAG CCCGTGGGTG 
AGCTTCCTAC GCAAAGGCCA GAGGCTCTGC ATCTATGGTC CAGCTTCACC ATCCTGGCGG 
GTTGTGGCCT CCAGCAAGAG CCGAAAAGTG CCCAGATACT TCATGCTCTC TGGGGCCTAC 
CAGGGCAAGC TCAAGCGGAG GCCCAGGGAG TTCTCCACCG CCTATGACCT TTTGGGGGCT 
CTCCAGCCAG GCCGGCCCCT CCGGGTGGTA GCCACAAAAG ACTGTGATGG GAATGAGGAA 
GAGAATCCTG ACTTTTCTTT CTTGGCTGTG GGTGATCGGC TGGAAGTGCT GAGGTCAGGC 
CAGGTCTGTG GGACCAAAGG CCAGGACATA GATGTCCTGG TATGTCAGCG GCTGAGTGAG 
CAGTCTGGGG AGGAAGAGGA GGACTTGGAG GAGATCGAGG ACGAGGCAGA GGACAAAGAG 
CAAATTCTGC TACCCCTCTA CCTCTCTGGG AGCTTTGTGG AGGAGGTGAA CGACAGCCGA 
CGCTATAACC TAGTGGATCT CACGGCCCAG TACTCCCTGC CCTGTGAGGT CAAGGTGGTG 
ACCAAGGATA CCCGTCACCC CACTGACCCC CTGGCTTCCT TCCCTGGCCT GCGGCTGGAG 
GAGAAGCTCA CAGAACCCTT TTTGGTGGTA AGCCTGGACT CCCAGCCAGA AATGTGCTTT 
GAGATCCCTC CCCGGTGGCT GGACCTGACT GTGGTGGAGG CTGAGGGACA GCCAGCCCAG 
GTGGCTCGGC CGCTGTCCAT CGCTCCGGTG GAGGAACTGT CAGAAGCTTT CTACTACAGC 
CTCCGGAAGC TGCCAGCCTC TGAGAGCCAA GCCCCCCCGC CCAGGCCCCC AAAGAGCCAG 
GGTATCAATA AGAAACAGCA GAACATACAG AGCTGTAAAG AGAGCAGTGT AAAGCCTCAA 
GTTGTAGAAC CGCAGAAATC CTGCCCGCAG CCTCAACTCA AGGCGAAGAC CTTGGAAGCC 
CTTCCCAAGA ACAAATCAAA TGTGTACAGC AAGATTTCTG TCCACAAGAA AGACCGCAAA 
CCCAACCCCC AAACACAGAA TTCAGTGCTG AGTATGAAGC CCAAAACCTC GAGCTCCCTC 
GGCAAGCACT CTACCATGGA GTCACATCTC CTGCCTGACC CGGATATGGA TGACCATGAC 
TATGAAGAAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248532.1
CDS	1091..2785
Translation	MPQNSTRWPIYFKSTQRIVTKASVVPEDQPLRLEAVEIHHGIRYARCVQVSKTKELLHLPLSQKGPFWRCKPSAPQTLHQILQDPALKDLTLSCPSLPWNSVILKPQYMLQAIMHMRSSIVKIPSTLEVEVEDVTASSQHIHFFKPLRLSEVLAGGGPFPLTTEILEVPEGPPVFLSPWVSFLRKGQRLCIYGPASPSWRVVASSKSRKVPRYFMLSGAYQGKLKRRPREFSTAYDLLGALQPGRPLRVVATKDCDGNEEENPDFSFLAVGDRLEVLRSGQVCGTKGQDIDVLVCQRLSEQSGEEEEDLEEIEDEAEDKEQILLPLYLSGSFVEEVNDSRRYNLVDLTAQYSLPCEVKVVTKDTRHPTDPLASFPGLRLEEKLTEPFLVVSLDSQPEMCFEIPPRWLDLTVVEAEGQPAQVARPLSIAPVEELSEAFYYSLRKLPASESQAPPPRPPKSQGINKKQQNIQSCKESSVKPQVVEPQKSCPQPQLKAKTLEALPKNKSNVYSKISVHKKDRKPNPQTQNSVLSMKPKTSSSLGKHSTMESHLLPDPDMDDHDYEEI

Target ORF information:

RefSeq Version	XM_011250230.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated family member 2 (Themis2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250230.2

ORF Insert Sequence:

ATGCCCCAGA ACTCCACCCG GTGGCCCATT TACTTCAAGT CAACTCAGAG AATTGTCACC 
AAGGCCAGTG TGGTGCCTGA GGATCAGCCC CTCAGACTTG AGGCTGTGGA AATACACCAT 
GGGATCCGCT ATGCGCGCTG CGTCCAGGTC TCAAAGACCA AGGAGCTCCT TCATCTGCCC 
CTTTCCCAGA AGGGACCCTT CTGGAGATGC AAGCCCAGTG CTCCACAAAC TCTGCACCAA 
ATCCTGCAGG ACCCAGCTCT GAAGGACCTC ACGCTCAGCT GCCCCAGCCT ACCCTGGAAC 
TCCGTGATCC TAAAACCCCA GTATATGCTC CAAGCCATCA TGCACATGCG CAGCAGCATC 
GTCAAGATCC CGTCTACGCT GGAGGTGGAG GTAGAAGACG TCACTGCTTC CTCCCAACAC 
ATCCACTTCT TCAAACCCTT GCGGCTGAGC GAGGTGTTGG CTGGGGGAGG CCCCTTCCCT 
CTGACCACGG AGATCCTGGA GGTTCCTGAG GGTCCGCCCG TCTTCCTCAG CCCGTGGGTG 
AGCTTCCTAC GCAAAGGCCA GAGGCTCTGC ATCTATGGTC CAGCTTCACC ATCCTGGCGG 
GTTGTGGCCT CCAGCAAGAG CCGAAAAGTG CCCAGATACT TCATGCTCTC TGGGGCCTAC 
CAGGGCAAGC TCAAGCGGAG GCCCAGGGAG TTCTCCACCG CCTATGACCT TTTGGGGGCT 
CTCCAGCCAG GCCGGCCCCT CCGGGTGGTA GCCACAAAAG ACTGTGATGG GAATGAGGAA 
GAGAATCCTG ACTTTTCTTT CTTGGCTGTG GGTGATCGGC TGGAAGTGCT GAGGTCAGGC 
CAGGTCTGTG GGACCAAAGG CCAGGACATA GATGTCCTGG TATGTCAGCG GCTGAGTGAG 
CAGTCTGGGG AGGAAGAGGA GGACTTGGAG GAGATCGAGG ACGAGGCAGA GGACAAAGAG 
CAAATTCTGC TACCCCTCTA CCTCTCTGGG AGCTTTGTGG AGGAGGTGAA CGACAGCCGA 
CGCTATAACC TAGTGGATCT CACGGCCCAG TACTCCCTGC CCTGTGAGGT CAAGGTGGTG 
ACCAAGGATA CCCGTCACCC CACTGACCCC CTGGCTTCCT TCCCTGGCCT GCGGCTGGAG 
GAGAAGCTCA CAGAACCCTT TTTGGTGGTA AGCCTGGACT CCCAGCCAGA AATGTGCTTT 
GAGATCCCTC CCCGGTGGCT GGACCTGACT GTGGTGGAGG CTGAGGGACA GCCAGCCCAG 
GTGGCTCGGC CGCTGTCCAT CGCTCCGGTG GAGGAACTGT CAGAAGCTTT CTACTACAGC 
CTCCGGAAGC TGCCAGCCTC TGAGAGCCAA GCCCCCCCGC CCAGGCCCCC AAAGAGCCAG 
GGTATCAATA AGAAACAGCA GAACATACAG AGCTGTAAAG AGAGCAGTGT AAAGCCTCAA 
GTTGTAGAAC CGCAGAAATC CTGCCCGCAG CCTCAACTCA AGGCGAAGAC CTTGGAAGCC 
CTTCCCAAGA ACAAATCAAA TGTGTACAGC AAGATTTCTG TCCACAAGAA AGACCGCAAA 
CCCAACCCCC AAACACAGAA TTCAGTGCTG AGTATGAAGC CCAAAACCTC GAGCTCCCTC 
GGCAAGCACT CTACCATGGA GTCACATCTC CTGCCTGACC CGGATATGGA TGACCATGAC 
TATGAAGAAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Themis2 ( XM_011250230.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248532.1 Mus musculus thymocyte selection associated family member 2 (Themis2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73533
Clone ID Related Accession (Same CDS sequence)	XM_011250230.3 , XM_011250230.2
Accession Version	XM_011250230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein THEMIS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA ACTCCACCCG GTGGCCCATT TACTTCAAGT CAACTCAGAG AATTGTCACC 
AAGGCCAGTG TGGTGCCTGA GGATCAGCCC CTCAGACTTG AGGCTGTGGA AATACACCAT 
GGGATCCGCT ATGCGCGCTG CGTCCAGGTC TCAAAGACCA AGGAGCTCCT TCATCTGCCC 
CTTTCCCAGA AGGGACCCTT CTGGAGATGC AAGCCCAGTG CTCCACAAAC TCTGCACCAA 
ATCCTGCAGG ACCCAGCTCT GAAGGACCTC ACGCTCAGCT GCCCCAGCCT ACCCTGGAAC 
TCCGTGATCC TAAAACCCCA GTATATGCTC CAAGCCATCA TGCACATGCG CAGCAGCATC 
GTCAAGATCC CGTCTACGCT GGAGGTGGAG GTAGAAGACG TCACTGCTTC CTCCCAACAC 
ATCCACTTCT TCAAACCCTT GCGGCTGAGC GAGGTGTTGG CTGGGGGAGG CCCCTTCCCT 
CTGACCACGG AGATCCTGGA GGTTCCTGAG GGTCCGCCCG TCTTCCTCAG CCCGTGGGTG 
AGCTTCCTAC GCAAAGGCCA GAGGCTCTGC ATCTATGGTC CAGCTTCACC ATCCTGGCGG 
GTTGTGGCCT CCAGCAAGAG CCGAAAAGTG CCCAGATACT TCATGCTCTC TGGGGCCTAC 
CAGGGCAAGC TCAAGCGGAG GCCCAGGGAG TTCTCCACCG CCTATGACCT TTTGGGGGCT 
CTCCAGCCAG GCCGGCCCCT CCGGGTGGTA GCCACAAAAG ACTGTGATGG GAATGAGGAA 
GAGAATCCTG ACTTTTCTTT CTTGGCTGTG GGTGATCGGC TGGAAGTGCT GAGGTCAGGC 
CAGGTCTGTG GGACCAAAGG CCAGGACATA GATGTCCTGG TATGTCAGCG GCTGAGTGAG 
CAGTCTGGGG AGGAAGAGGA GGACTTGGAG GAGATCGAGG ACGAGGCAGA GGACAAAGAG 
CAAATTCTGC TACCCCTCTA CCTCTCTGGG AGCTTTGTGG AGGAGGTGAA CGACAGCCGA 
CGCTATAACC TAGTGGATCT CACGGCCCAG TACTCCCTGC CCTGTGAGGT CAAGGTGGTG 
ACCAAGGATA CCCGTCACCC CACTGACCCC CTGGCTTCCT TCCCTGGCCT GCGGCTGGAG 
GAGAAGCTCA CAGAACCCTT TTTGGTGGTA AGCCTGGACT CCCAGCCAGA AATGTGCTTT 
GAGATCCCTC CCCGGTGGCT GGACCTGACT GTGGTGGAGG CTGAGGGACA GCCAGCCCAG 
GTGGCTCGGC CGCTGTCCAT CGCTCCGGTG GAGGAACTGT CAGAAGCTTT CTACTACAGC 
CTCCGGAAGC TGCCAGCCTC TGAGAGCCAA GCCCCCCCGC CCAGGCCCCC AAAGAGCCAG 
GGTATCAATA AGAAACAGCA GAACATACAG AGCTGTAAAG AGAGCAGTGT AAAGCCTCAA 
GTTGTAGAAC CGCAGAAATC CTGCCCGCAG CCTCAACTCA AGGCGAAGAC CTTGGAAGCC 
CTTCCCAAGA ACAAATCAAA TGTGTACAGC AAGATTTCTG TCCACAAGAA AGACCGCAAA 
CCCAACCCCC AAACACAGAA TTCAGTGCTG AGTATGAAGC CCAAAACCTC GAGCTCCCTC 
GGCAAGCACT CTACCATGGA GTCACATCTC CTGCCTGACC CGGATATGGA TGACCATGAC 
TATGAAGAAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248532.1
CDS	1078..2772
Translation	MPQNSTRWPIYFKSTQRIVTKASVVPEDQPLRLEAVEIHHGIRYARCVQVSKTKELLHLPLSQKGPFWRCKPSAPQTLHQILQDPALKDLTLSCPSLPWNSVILKPQYMLQAIMHMRSSIVKIPSTLEVEVEDVTASSQHIHFFKPLRLSEVLAGGGPFPLTTEILEVPEGPPVFLSPWVSFLRKGQRLCIYGPASPSWRVVASSKSRKVPRYFMLSGAYQGKLKRRPREFSTAYDLLGALQPGRPLRVVATKDCDGNEEENPDFSFLAVGDRLEVLRSGQVCGTKGQDIDVLVCQRLSEQSGEEEEDLEEIEDEAEDKEQILLPLYLSGSFVEEVNDSRRYNLVDLTAQYSLPCEVKVVTKDTRHPTDPLASFPGLRLEEKLTEPFLVVSLDSQPEMCFEIPPRWLDLTVVEAEGQPAQVARPLSIAPVEELSEAFYYSLRKLPASESQAPPPRPPKSQGINKKQQNIQSCKESSVKPQVVEPQKSCPQPQLKAKTLEALPKNKSNVYSKISVHKKDRKPNPQTQNSVLSMKPKTSSSLGKHSTMESHLLPDPDMDDHDYEEI

Target ORF information:

RefSeq Version	XM_011250230.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated family member 2 (Themis2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250230.3

ORF Insert Sequence:

ATGCCCCAGA ACTCCACCCG GTGGCCCATT TACTTCAAGT CAACTCAGAG AATTGTCACC 
AAGGCCAGTG TGGTGCCTGA GGATCAGCCC CTCAGACTTG AGGCTGTGGA AATACACCAT 
GGGATCCGCT ATGCGCGCTG CGTCCAGGTC TCAAAGACCA AGGAGCTCCT TCATCTGCCC 
CTTTCCCAGA AGGGACCCTT CTGGAGATGC AAGCCCAGTG CTCCACAAAC TCTGCACCAA 
ATCCTGCAGG ACCCAGCTCT GAAGGACCTC ACGCTCAGCT GCCCCAGCCT ACCCTGGAAC 
TCCGTGATCC TAAAACCCCA GTATATGCTC CAAGCCATCA TGCACATGCG CAGCAGCATC 
GTCAAGATCC CGTCTACGCT GGAGGTGGAG GTAGAAGACG TCACTGCTTC CTCCCAACAC 
ATCCACTTCT TCAAACCCTT GCGGCTGAGC GAGGTGTTGG CTGGGGGAGG CCCCTTCCCT 
CTGACCACGG AGATCCTGGA GGTTCCTGAG GGTCCGCCCG TCTTCCTCAG CCCGTGGGTG 
AGCTTCCTAC GCAAAGGCCA GAGGCTCTGC ATCTATGGTC CAGCTTCACC ATCCTGGCGG 
GTTGTGGCCT CCAGCAAGAG CCGAAAAGTG CCCAGATACT TCATGCTCTC TGGGGCCTAC 
CAGGGCAAGC TCAAGCGGAG GCCCAGGGAG TTCTCCACCG CCTATGACCT TTTGGGGGCT 
CTCCAGCCAG GCCGGCCCCT CCGGGTGGTA GCCACAAAAG ACTGTGATGG GAATGAGGAA 
GAGAATCCTG ACTTTTCTTT CTTGGCTGTG GGTGATCGGC TGGAAGTGCT GAGGTCAGGC 
CAGGTCTGTG GGACCAAAGG CCAGGACATA GATGTCCTGG TATGTCAGCG GCTGAGTGAG 
CAGTCTGGGG AGGAAGAGGA GGACTTGGAG GAGATCGAGG ACGAGGCAGA GGACAAAGAG 
CAAATTCTGC TACCCCTCTA CCTCTCTGGG AGCTTTGTGG AGGAGGTGAA CGACAGCCGA 
CGCTATAACC TAGTGGATCT CACGGCCCAG TACTCCCTGC CCTGTGAGGT CAAGGTGGTG 
ACCAAGGATA CCCGTCACCC CACTGACCCC CTGGCTTCCT TCCCTGGCCT GCGGCTGGAG 
GAGAAGCTCA CAGAACCCTT TTTGGTGGTA AGCCTGGACT CCCAGCCAGA AATGTGCTTT 
GAGATCCCTC CCCGGTGGCT GGACCTGACT GTGGTGGAGG CTGAGGGACA GCCAGCCCAG 
GTGGCTCGGC CGCTGTCCAT CGCTCCGGTG GAGGAACTGT CAGAAGCTTT CTACTACAGC 
CTCCGGAAGC TGCCAGCCTC TGAGAGCCAA GCCCCCCCGC CCAGGCCCCC AAAGAGCCAG 
GGTATCAATA AGAAACAGCA GAACATACAG AGCTGTAAAG AGAGCAGTGT AAAGCCTCAA 
GTTGTAGAAC CGCAGAAATC CTGCCCGCAG CCTCAACTCA AGGCGAAGAC CTTGGAAGCC 
CTTCCCAAGA ACAAATCAAA TGTGTACAGC AAGATTTCTG TCCACAAGAA AGACCGCAAA 
CCCAACCCCC AAACACAGAA TTCAGTGCTG AGTATGAAGC CCAAAACCTC GAGCTCCCTC 
GGCAAGCACT CTACCATGGA GTCACATCTC CTGCCTGACC CGGATATGGA TGACCATGAC 
TATGAAGAAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Themis2 lowers the threshold for B cell activation during positive selection.
Nature immunology18(2)205-213(2017 Feb)
Cheng D,Deobagkar-Lele M,Zvezdova E,Choi S,Uehara S,Baup D,Bennett SC,Bull KR,Crockford TL,Ferry H,Warzecha C,Marcellin M,de Peredo AG,Lesourne R,Anzilotti C,Love PE,Cornall RJ

Themis2 is not required for B cell development, activation, and antibody responses.
Journal of immunology (Baltimore, Md. : 1950)193(2)700-7(2014 Jul)
Hartweger H,Schweighoffer E,Davidson S,Peirce MJ,Wack A,Tybulewicz VL

Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.
Journal of immunology (Baltimore, Md. : 1950)189(3)1154-61(2012 Aug)
Lesourne R,Zvezdova E,Song KD,El-Khoury D,Uehara S,Barr VA,Samelson LE,Love PE

Themis2/ICB1 is a signaling scaffold that selectively regulates macrophage Toll-like receptor signaling and cytokine production.
PloS one5(7)e11465(2010 Jul)
Peirce MJ,Brook M,Morrice N,Snelgrove R,Begum S,Lanfrancotti A,Notley C,Hussell T,Cope AP,Wait R

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Themis2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

SUPPORT

COMPANY

SUPPORT

COMPANY