Banf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Banf1
Entrez Gene ID	23825
Full Name	barrier to autointegration factor 1
Synonyms	Baf,Bcrp1,L2bp1/Baf
General protein information	Preferred Names barrier to autointegration factor 1 Names barrier-to-autointegration factor LAP2-binding protein 1 breakpoint cluster region protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248628.1	XP_011246930.1	barrier-to-autointegration factor isoform X1
NM_001286608.1	NP_001273537.1	barrier-to-autointegration factor
NM_011793.3	NP_035923.1	barrier-to-autointegration factor
NM_001038231.2	NP_001033320.1	barrier-to-autointegration factor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	banf1	NP_991125.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008753	XP_314872.1
Rattus norvegicus (Norway rat)	Banf1	NP_446083.1
Drosophila melanogaster (fruit fly)	baf	NP_609176.1
Pan troglodytes (chimpanzee)	BANF1	XP_003339255.1
Canis lupus familiaris (dog)	BANF1	XP_854776.1
Xenopus tropicalis (tropical clawed frog)	banf1	XP_002940546.1
Homo sapiens (human)	BANF1	NP_003851.1
Mus musculus (house mouse)	Banf1	NP_001033320.1
Caenorhabditis elegans (roundworm)	baf-1	NP_499085.1
	BANF1	XP_001111884.2
Bos taurus (cattle)	BANF1	NP_892033.1

Gene Ontology
Bibliography

Related articles in PubMed

Barrier to auto integration factor becomes dephosphorylated during HSV-1 Infection and Can Act as a host defense by impairing viral DNA replication and gene expression.
Jamin A, Thunuguntla P, Wicklund A, Jones C, Wiebe MS
PloS one9(6)e100511(2014)

Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells.
Cox JL, Mallanna SK, Ormsbee BD, Desler M, Wiebe MS, Rizzino A
Journal of cell science124(Pt 15)2654-65(2011 Aug)

Compartment-specific roles of ATP-binding cassette transporters define differential topotecan distribution in brain parenchyma and cerebrospinal fluid.
Shen J, Carcaboso AM, Hubbard KE, Tagen M, Wynn HG, Panetta JC, Waters CM, Elmeliegy MA, Stewart CF
Cancer research69(14)5885-92(2009 Jul)

Mutagenesis of diploid mammalian genes by gene entrapment.
Lin Q, Donahue SL, Moore-Jarrett T, Cao S, Osipovich AB, Ruley HE
Nucleic acids research34(20)e139(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BAF can act in a phosphorylation-regulated manner to impair HSV-1 transcription and/or DNA replication.
Title: Barrier to auto integration factor becomes dephosphorylated during HSV-1 Infection and Can Act as a host defense by impairing viral DNA replication and gene expression.

Data determined that the knockdown of Banf1 alters the cell cycle distribution of both human and mouse ESCs by causing an uncharacteristic increase in the proportion of cells in the G2-M phase of the cell cycle.
Title: Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells.

Results suggest that Bcrp1 and P-gp transport topotecan into vCSF and out of brain parenchyma through the blood-brain barrier, and findings may help to improve pharmacologic strategies to treat brain tumors.
Title: Compartment-specific roles of ATP-binding cassette transporters define differential topotecan distribution in brain parenchyma and cerebrospinal fluid.

LAP2alpha stabilizes the association of BAF with the PIC to stimulate intermolecular integration and suppress autointegration.
Title: LAP2alpha and BAF collaborate to organize the Moloney murine leukemia virus preintegration complex.

testicular cells display a 'Side Population' that express Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in hematopoietic cells
Title: 'Side Population' cells in adult mouse testis express Bcrp1 gene and are enriched in spermatogonia and germinal stem cells.

The following Banf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Banf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00189	NM_001038231.2 Latest version!	Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu00189	NM_011793.3 Latest version!	Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu00189	NM_001286608.1 Latest version!	Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu00189	XM_011248628.1 Latest version!	Mus musculus barrier to autointegration factor 1 (Banf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Banf1 ( NM_001286608.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273537.1 Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu00189
Clone ID Related Accession (Same CDS sequence)	XM_011248628.1 , NM_001286608.1 , NM_011793.3 , NM_001038231.2
Accession Version	NM_001286608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	barrier-to-autointegration factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE863097.1, AK011079.1, BY111854.1 and AC122861.4. Transcript Variant: This variant (3) represents the longest transcript and encodes the functional protein. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN840378.1, AA222472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273537.1
CDS	460..729
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGDVLSKRLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	NM_001286608.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286608.1

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Banf1 ( NM_011793.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035923.1 Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00189
Clone ID Related Accession (Same CDS sequence)	XM_011248628.1 , NM_001286608.1 , NM_011793.3 , NM_001038231.2
Accession Version	NM_011793.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	barrier-to-autointegration factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE863097.1, AK011079.1 and AC122861.4. On Nov 8, 2013 this sequence version replaced NM_011793.2. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 3. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI408056.1, BI525512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035923.1
CDS	326..595
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGDVLSKRLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	NM_011793.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011793.3

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Banf1 ( NM_001038231.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001033320.1 Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00189
Clone ID Related Accession (Same CDS sequence)	XM_011248628.1 , NM_001286608.1 , NM_011793.3 , NM_001038231.2
Accession Version	NM_001038231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	barrier-to-autointegration factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122861.4 and AK010257.1. On Nov 8, 2013 this sequence version replaced NM_001038231.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 3. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU935633.1, AK010257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033320.1
CDS	118..387
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGDVLSKRLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	NM_001038231.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BAF nuclear assembly factor 1 (Banf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038231.2

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Banf1 ( XM_011248628.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246930.1 Mus musculus barrier to autointegration factor 1 (Banf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu00189
Clone ID Related Accession (Same CDS sequence)	XM_011248628.1 , NM_001286608.1 , NM_011793.3 , NM_001038231.2
Accession Version	XM_011248628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	barrier-to-autointegration factor isoform X1
Comment

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246930.1
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGDVLSKRLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	XM_011248628.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus barrier to autointegration factor 1 (Banf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248628.1

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA AAAGCCAGTG 
GGGAGCCTGG CCGGGATTGG TGACGTCCTG AGCAAGAGGC TGGAGGAAAG GGGCTTTGAC 
AAGGCTTATG TGGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGA 
GAATGGCTGA AGGATACATG TGGTGCCAAT GCCAAGCAGT CCCGGGACTG CTTTGGGTGC 
CTTCGAGAAT GGTGTGATGC CTTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Barrier to auto integration factor becomes dephosphorylated during HSV-1 Infection and Can Act as a host defense by impairing viral DNA replication and gene expression.
PloS one9(6)e100511(2014)
Jamin A,Thunuguntla P,Wicklund A,Jones C,Wiebe MS

Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells.
Journal of cell science124(Pt 15)2654-65(2011 Aug)
Cox JL,Mallanna SK,Ormsbee BD,Desler M,Wiebe MS,Rizzino A

Compartment-specific roles of ATP-binding cassette transporters define differential topotecan distribution in brain parenchyma and cerebrospinal fluid.
Cancer research69(14)5885-92(2009 Jul)
Shen J,Carcaboso AM,Hubbard KE,Tagen M,Wynn HG,Panetta JC,Waters CM,Elmeliegy MA,Stewart CF

Mutagenesis of diploid mammalian genes by gene entrapment.
Nucleic acids research34(20)e139(2006)
Lin Q,Donahue SL,Moore-Jarrett T,Cao S,Osipovich AB,Ruley HE

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Banf1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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