Cetn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Cetn1
Entrez Gene ID	26369
Full Name	centrin 1
Synonyms	caltractin
General protein information	Preferred Names centrin 1 Names centrin-1 testis-specific murine centrin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 A1

mRNA and Protein(s)

mRNA Protein Name

NM_007593.5 NP_031619.3 centrin-1
Pathways from BioSystems

mRNA	Protein	Name
NM_007593.5	NP_031619.3	centrin-1

Homologies

Canis lupus familiaris (dog)	CETN1	XP_547653.1
Pan troglodytes (chimpanzee)	CETN1	XP_523881.1
	CETN1	XP_001089502.1
Arabidopsis thaliana (thale cress)	CEN2	NP_001031798.1
Bos taurus (cattle)	CETN1	NP_001072974.1
Mus musculus (house mouse)	Cetn1	NP_031619.3
Danio rerio (zebrafish)	cetn2	XP_002667227.2
Homo sapiens (human)	CETN1	NP_004057.1
Rattus norvegicus (Norway rat)	Cetn1	NP_445913.1
Gallus gallus (chicken)	CETN1	NP_001264849.1
Oryza sativa (rice)	Os10g0389000	NP_001064498.2

Gene Ontology
Bibliography

Related articles in PubMed

Crystal Structure of Wild-Type Centrin 1 from Mus musculus Occupied by Ca2.
Kim SY, Kim DS, Hong JE, Park JH
Biochemistry. Biokhimiia82(10)1129-1139(2017 Oct)

Centrosome Amplification Is Sufficient to Promote Spontaneous Tumorigenesis in Mammals.
Levine MS, Bakker B, Boeckx B, Moyett J, Lu J, Vitre B, Spierings DC, Lansdorp PM, Cleveland DW, Lambrechts D, Foijer F, Holland AJ
Developmental cell40(3)313-322.e5(2017 02)

Generation and Characterization of a Tissue-Specific Centrosome Indicator Mouse Line.
Hirai M, Chen J, Evans SM
Genesis (New York, N.Y. : 2000)54(5)286-96(2016 05)

The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly.
Kobayashi T, Kim S, Lin YC, Inoue T, Dynlacht BD
The Journal of cell biology204(2)215-29(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure exhibits three structural features, i.e. four Ca2+ equally occupied at each EF-hand motif, structural changes accompanying helix motion at the N- and C-lobes, and adoption of N-C type dimerization when Ca2+ binds to EF-hand I and II in the N-lobe.
Title: Crystal Structure of Wild-Type Centrin 1 from Mus musculus Occupied by Ca2.

Experiments utilizing mouse embryo fibroblasts (MEFs) demonstrated the feasibility of real-time imaging, and showed that EGFP-Centrin1 expression mirrored the endogenous centrosome cycle, undergoing precisely one round of duplication through the cell cycle. Moreover, experiments using embryo and adult mouse tissues demonstrated that EGFP-Centrin1 specifically mirrors the localization of endogenous centrosomes.
Title: Generation and Characterization of a Tissue-Specific Centrosome Indicator Mouse Line.

role in late steps of spermiogenesis and spermatid maturation
Title: Germline deletion of Cetn1 causes infertility in male mice.

Immunostaining of seminiferous tubules revealed that cells in layer containing spermatogonia showed colocalization of SUMO-2/3 with centrin-1 in cytoplasmic spots. Binding of centrin-1 and SUMO-2/3 appears to be calcium dependent.
Title: An EF-hands protein, centrin-1, is an EGTA-sensitive SUMO-interacting protein in mouse testis.

Cen1 and Cen2 contribute to the centrin-transducin complex and potentially participate in the regulation of transducin translocation through the photoreceptor cilium
Title: Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells.

The following Cetn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cetn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58218	NM_007593.5 Latest version!	Mus musculus centrin 1 (Cetn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cetn1 ( NM_007593.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031619.3 Mus musculus centrin 1 (Cetn1), mRNA.

Price & Availability↑

CloneID	OMu58218
Clone ID Related Accession (Same CDS sequence)	NM_007593.5
Accession Version	NM_007593.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	centrin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006940.1 and AK015808.1. On Jun 15, 2006 this sequence version replaced NM_007593.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AK006940.1 [ECO:0000345] ##Evidence-Data-END##

ATGGCGTCCA CCTTCAGGAA GTCAAACGTT GCCTCTACCA GCTACAAGAG AAAGGTGGGT 
CCTAAGCCTG AACTCACCGA AGATCAAAAG CAAGAAGTTC GGGAAGCCTT TGACCTCTTC 
GATTCTGATG GGAGCGGGAC CATCGATGTG AAGGAGCTGA AGGTGGCCAT GAGAGCACTT 
GGCTTTGAAC CCAGGAAGGA AGAGATGAAG AAAATGATTT CAGAAGTAGA CAAAGAGGCC 
ACAGGAAAGA TCAGCTTCAA TGACTTCTTG GCTGTGATGA CTCAGAAGAT GGCCGAGAAA 
GATACCAAAG AGGAAATCCT GAAGGCTTTC AGGTTGTTTG ATGACGACGA AACTGGGAAA 
ATTTCATTCA AAAACCTCAA GCGTGTGGCC AATGAGCTGG GGGAAAGCCT CACAGACGAG 
GAGCTGCAGG AAATGATTGA TGAAGCTGAT CGTGATGGCG ACGGAGAAGT GAACGAGGAA 
GAGTTTCTTA AGATCATGAA AAAGACCAAC CTTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031619.3
CDS	51..569
Translation	MASTFRKSNVASTSYKRKVGPKPELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGESLTDEELQEMIDEADRDGDGEVNEEEFLKIMKKTNLY

Target ORF information:

RefSeq Version	NM_007593.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrin 1 (Cetn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007593.5

ORF Insert Sequence:

ATGGCGTCCA CCTTCAGGAA GTCAAACGTT GCCTCTACCA GCTACAAGAG AAAGGTGGGT 
CCTAAGCCTG AACTCACCGA AGATCAAAAG CAAGAAGTTC GGGAAGCCTT TGACCTCTTC 
GATTCTGATG GGAGCGGGAC CATCGATGTG AAGGAGCTGA AGGTGGCCAT GAGAGCACTT 
GGCTTTGAAC CCAGGAAGGA AGAGATGAAG AAAATGATTT CAGAAGTAGA CAAAGAGGCC 
ACAGGAAAGA TCAGCTTCAA TGACTTCTTG GCTGTGATGA CTCAGAAGAT GGCCGAGAAA 
GATACCAAAG AGGAAATCCT GAAGGCTTTC AGGTTGTTTG ATGACGACGA AACTGGGAAA 
ATTTCATTCA AAAACCTCAA GCGTGTGGCC AATGAGCTGG GGGAAAGCCT CACAGACGAG 
GAGCTGCAGG AAATGATTGA TGAAGCTGAT CGTGATGGCG ACGGAGAAGT GAACGAGGAA 
GAGTTTCTTA AGATCATGAA AAAGACCAAC CTTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal Structure of Wild-Type Centrin 1 from Mus musculus Occupied by Ca2.
Biochemistry. Biokhimiia82(10)1129-1139(2017 Oct)
Kim SY,Kim DS,Hong JE,Park JH

Centrosome Amplification Is Sufficient to Promote Spontaneous Tumorigenesis in Mammals.
Developmental cell40(3)313-322.e5(2017 02)
Levine MS,Bakker B,Boeckx B,Moyett J,Lu J,Vitre B,Spierings DC,Lansdorp PM,Cleveland DW,Lambrechts D,Foijer F,Holland AJ

Generation and Characterization of a Tissue-Specific Centrosome Indicator Mouse Line.
Genesis (New York, N.Y. : 2000)54(5)286-96(2016 05)
Hirai M,Chen J,Evans SM

The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly.
The Journal of cell biology204(2)215-29(2014 Jan)
Kobayashi T,Kim S,Lin YC,Inoue T,Dynlacht BD

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Cetn1 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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