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Usp25 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Usp25
Entrez Gene ID 30940
Full Name ubiquitin specific peptidase 25
General protein information
Preferred Names

ubiquitin specific peptidase 25

Names

ubiquitin carboxyl-terminal hydrolase 25
deubiquitinating enzyme 25
mUSP25
ubiquitin specific protease 25
ubiquitin thioesterase 25
ubiquitin thiolesterase 25
ubiquitin-specific-processing protease 25
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

16

16|16 C3.1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006523056.2 XP_006523119.1 ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_006523055.2 XP_006523118.1 ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_006523056.3 XP_006523119.1 ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_006523057.1 XP_006523120.1 ubiquitin carboxyl-terminal hydrolase 25 isoform X3
NM_013918.2 NP_038946.2 ubiquitin carboxyl-terminal hydrolase 25
XM_006523055.3 XP_006523118.1 ubiquitin carboxyl-terminal hydrolase 25 isoform X1

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Usp25 NP_038946.2
Rattus norvegicus (Norway rat) Usp25 NP_001100584.1
Homo sapiens (human) USP25 NP_001269970.1
USP25 XP_001107973.2
Canis lupus familiaris (dog) USP25 XP_535562.3
Xenopus tropicalis (tropical clawed frog) usp25 NP_001039152.1
Bos taurus (cattle) USP25 XP_005201215.1
Gallus gallus (chicken) USP25 XP_004938410.1
Pan troglodytes (chimpanzee) USP25 XP_531393.2
Danio rerio (zebrafish) usp25 NP_001001886.1

Gene Ontology


Bibliography

Related articles in PubMed

The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.
Ren Y, Zhao Y, Lin D, Xu X, Zhu Q, Yao J, Shu HB, Zhong B
The Journal of biological chemistry291(25)13206-15(2016 Jun)

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S
Proceedings of the National Academy of Sciences of the United States of America113(6)E705-14(2016 Feb)

Induction of USP25 by viral infection promotes innate antiviral responses by mediating the stabilization of TRAF3 and TRAF6.
Lin D, Zhang M, Zhang MX, Ren Y, Jin J, Zhao Q, Pan Z, Wu M, Shu HB, Dong C, Zhong B
Proceedings of the National Academy of Sciences of the United States of America112(36)11324-9(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IRF7 directly binds to the two conserved IRF binding sites on the USP25 promoter to drive transcription of Usp25.
Title: The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.

Data show that the degradation of TNF receptor-associated factor 3 (TRAF3) and TNF receptor-associated Ffactor 6 (TRAF6) was accelerated in ubiquitin-specific protease 25 knockout (USP25-/-) cells after viral infection.
Title: Induction of USP25 by viral infection promotes innate antiviral responses by mediating the stabilization of TRAF3 and TRAF6.

inhibiting the degradation of TRAF3 during TLR4 activation, USP25 enables a balanced innate immune response.
Title: Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3.

USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling.
Title: Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25.

The following Usp25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu52772 XM_006523057.1
Latest version!		 Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu10159 NM_013918.2
Latest version!		 Mus musculus ubiquitin specific peptidase 25 (Usp25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OMu52770 XM_006523055.3
Latest version!		 Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu52771 XM_006523056.3
Latest version!		 Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OMu52771 XM_006523056.2 Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu52770 XM_006523055.2 Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu52772
Clone ID Related Accession (Same CDS sequence) XM_006523057.1
Accession Version XM_006523057.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCGGTC AGTATCCACT TCAGGTGAAC GGGTTCAAAG ACCTGCATGA GTGCCTGGAG 
GCCGCCATGA TCGAAGGGGA GATCGAATCC TTACACTCCG ATAACTCAGG AAAGTCAGGC 
CAGGAGCATT GGTTCACTGA ATTGCCGCCG GTATTAACAT TTGAACTGTC AAGATTTGAA 
TTTAACCAGG CATTGGGAAG ACCAGAAAAA ATTCACAATA AACTAGAATT TCCCCAAGTT 
TTATATCTGG ACAGGTATAT GCACAGAAAT AGAGAAATAA CAAGAATTAA ACGTGAAGAG 
ATCAAGAGAC TTAAAGATTA CCTCACAGTA TTACAACAAA GATTAGAGAG ATATTTAAGC 
TATGGTTCCG GTCCCAAAAG ATTCCCATTG GTAGATGTTC TACAGTATGC ATTGGAGTTT 
GCCTCCAGTA AACCTGTGTG CACTTCCCCC GTTGATGACA TCGATGCTAG TTCCTCAGCA 
AGTGGCCCCC TACCATCACA GTCCTTACCA AGCACAACAG AACAGCAGGG ACCCTGTGCT 
TCAGATCTGC CCGGCTCCTC CTCACCCGCA TCAGGTGCCG CCCTGCCCTT GAGGTCAGTG 
ATTCACAAAC CCTTCACTCA GTCTCGGATA CCTCCAGATT TGCCCATGCA TCCGGCACCA 
AGGCACATCA CAGAGGAAGA ACTGTGCGTG CTGGAGAGCT GTTTACATCG CTGGAGGACG 
GAAATAGAAA ATGACACGCG AGATCTGCAG GAAAGCATAT CCAGAATCCA TCGAACAATT 
GAACTCATGT ACTCGGACAA ATCCATGATC CAAGTCCCTT ACCGCCTCCA TGCCGTCCTA 
GTTCATGAGG GCCAGGCTAA TGCCGGCCAC TACTGGGCGT ACATCTTTGA CCACCGGGAG 
AGCCGGTGGA TGAAGTATAA TGATATTGCT GTGACCAAGT CTTCATGGGA AGAGCTAGTG 
AGGGACTCTT TCGGTGGTTA TCGAAATGCC AGTGCATACT GCTTAATGTA CATAGACGAC 
AAGGCACAGT TCCTAATACA AGAGGAGTTT AATAAAGAGA CTGGGCAGGC CCTTGTTGGA 
ATGGAAACCT TACCTCCAGA CTTGAGAGAC TTTGTTGAGG AGGACAACCA GCGCTTTGAG 
AAGGAACTGG AAGAATGGGA CACGCAGCTT GCCCAACGAT CACTGCAAGA AAAGCTGCTA 
GCTGCGCCGA AACTGCGGGA AGCAGAGGCT TCGGCCACCA CAGCACAAGC AGGAGGCGCA 
GACTATCTAG AGCAGCCATC AAGAAGTGAC TTGTCAAAGC ACTGGAAAGA AGAAACGCTC 
CGAGTGATTG CCAAGGCGTC ACATGATCTT GAAGATAAAG GGCCAGAGAC AGTTTTGCAG 
TCGAAACCTG CAAACACCAC GAGCCAACCA CCTTCTAGCC AGCAAGGTGT AGAGGTGGAG 
ATTCCCCACT TAGGGAAATT TGTGATTGAG GCAAAAGAGG GAGGGTACGA TGACGAGCAA 
GCTTCCTTTC AACCCCCGTG GCAGGTCATG ATGACCCCCA GTATGCAAGG CGTTATCATG 
GCGATAGGTA AATCCAGGAG TGTGTATGAC AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG 
GCAATTAAAT TAGAGTATTC TAGGCTGGTT AAGTTGGCCC AAGAAGATAC TCCACCAGAA 
ACAGATTATC GTTTACATCA TGTCCTGGTC TACTTTATCC AGAACCAGGC ACCAAAGAAA 
ATCATTGAGA AAACATTACT CGAACAATTT GGAGATAGAA ATTTGAGTTT TGATGAAAGG 
TGTCACAACA TAATGAAAGT TGCCCAAGCC AAACTGGAAA TGATCAAACC TGAAGAAGTG 
AACTTGGAAG AATATGAGGA GTGGCATGCA GATTATAAGA AATTCCGAGA AACAACTATG 
TATCTCATAA CTGGACTAGA AAACTTTCAG AGAGAAAGTT ATATAGACTC CTTGCTGTTC 
CTTCTCTGTG CTTATCAGAA TAACAAAGAG CTCTTGTCTA AGGGTCCGTA CAGAGGCCAC 
GATGGGGAGC TGATATCACA TTACAGAAGA GAGTGCTTAC TGAAATTAAA CGAGCAAGCG 
GCCGAGCTGT TTGAGTCTGG AGAGGACGGA GACGTGAACA ATGGCTTAAT TATTATGAAT 
GAGTTTATCG TTCCCTTTCT GCCCTTGTTG CTGGTGGATG ACATGGAAGA GAAAGATATC 
CTTGCTGTGG AAGACATGAG GAATCGCTGG TGTTCATACC TGGGGCAAGA AATGGAAGCA 
AACCTCCAAG AAAAGCTCAC GGATTTTCTG CCAAAACTGC TTGATTGTTC TACAGAGATT 
AAAGGTTTCC ACGAGCCACC AAAGTTACCT TCCTACTCTG CGCACGAACT CTGTGAGCGA 
TTCGCCCGGA TCATGTTGTC CCTCAGTCGT ACGCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006523120.1
CDS97..2547
Translation

Target ORF information:

RefSeq Version XM_006523057.1
Organism Mus musculus(house mouse)
Definition Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006523057.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCGGTC AGTATCCACT TCAGGTGAAC GGGTTCAAAG ACCTGCATGA GTGCCTGGAG 
GCCGCCATGA TCGAAGGGGA GATCGAATCC TTACACTCCG ATAACTCAGG AAAGTCAGGC 
CAGGAGCATT GGTTCACTGA ATTGCCGCCG GTATTAACAT TTGAACTGTC AAGATTTGAA 
TTTAACCAGG CATTGGGAAG ACCAGAAAAA ATTCACAATA AACTAGAATT TCCCCAAGTT 
TTATATCTGG ACAGGTATAT GCACAGAAAT AGAGAAATAA CAAGAATTAA ACGTGAAGAG 
ATCAAGAGAC TTAAAGATTA CCTCACAGTA TTACAACAAA GATTAGAGAG ATATTTAAGC 
TATGGTTCCG GTCCCAAAAG ATTCCCATTG GTAGATGTTC TACAGTATGC ATTGGAGTTT 
GCCTCCAGTA AACCTGTGTG CACTTCCCCC GTTGATGACA TCGATGCTAG TTCCTCAGCA 
AGTGGCCCCC TACCATCACA GTCCTTACCA AGCACAACAG AACAGCAGGG ACCCTGTGCT 
TCAGATCTGC CCGGCTCCTC CTCACCCGCA TCAGGTGCCG CCCTGCCCTT GAGGTCAGTG 
ATTCACAAAC CCTTCACTCA GTCTCGGATA CCTCCAGATT TGCCCATGCA TCCGGCACCA 
AGGCACATCA CAGAGGAAGA ACTGTGCGTG CTGGAGAGCT GTTTACATCG CTGGAGGACG 
GAAATAGAAA ATGACACGCG AGATCTGCAG GAAAGCATAT CCAGAATCCA TCGAACAATT 
GAACTCATGT ACTCGGACAA ATCCATGATC CAAGTCCCTT ACCGCCTCCA TGCCGTCCTA 
GTTCATGAGG GCCAGGCTAA TGCCGGCCAC TACTGGGCGT ACATCTTTGA CCACCGGGAG 
AGCCGGTGGA TGAAGTATAA TGATATTGCT GTGACCAAGT CTTCATGGGA AGAGCTAGTG 
AGGGACTCTT TCGGTGGTTA TCGAAATGCC AGTGCATACT GCTTAATGTA CATAGACGAC 
AAGGCACAGT TCCTAATACA AGAGGAGTTT AATAAAGAGA CTGGGCAGGC CCTTGTTGGA 
ATGGAAACCT TACCTCCAGA CTTGAGAGAC TTTGTTGAGG AGGACAACCA GCGCTTTGAG 
AAGGAACTGG AAGAATGGGA CACGCAGCTT GCCCAACGAT CACTGCAAGA AAAGCTGCTA 
GCTGCGCCGA AACTGCGGGA AGCAGAGGCT TCGGCCACCA CAGCACAAGC AGGAGGCGCA 
GACTATCTAG AGCAGCCATC AAGAAGTGAC TTGTCAAAGC ACTGGAAAGA AGAAACGCTC 
CGAGTGATTG CCAAGGCGTC ACATGATCTT GAAGATAAAG GGCCAGAGAC AGTTTTGCAG 
TCGAAACCTG CAAACACCAC GAGCCAACCA CCTTCTAGCC AGCAAGGTGT AGAGGTGGAG 
ATTCCCCACT TAGGGAAATT TGTGATTGAG GCAAAAGAGG GAGGGTACGA TGACGAGCAA 
GCTTCCTTTC AACCCCCGTG GCAGGTCATG ATGACCCCCA GTATGCAAGG CGTTATCATG 
GCGATAGGTA AATCCAGGAG TGTGTATGAC AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG 
GCAATTAAAT TAGAGTATTC TAGGCTGGTT AAGTTGGCCC AAGAAGATAC TCCACCAGAA 
ACAGATTATC GTTTACATCA TGTCCTGGTC TACTTTATCC AGAACCAGGC ACCAAAGAAA 
ATCATTGAGA AAACATTACT CGAACAATTT GGAGATAGAA ATTTGAGTTT TGATGAAAGG 
TGTCACAACA TAATGAAAGT TGCCCAAGCC AAACTGGAAA TGATCAAACC TGAAGAAGTG 
AACTTGGAAG AATATGAGGA GTGGCATGCA GATTATAAGA AATTCCGAGA AACAACTATG 
TATCTCATAA CTGGACTAGA AAACTTTCAG AGAGAAAGTT ATATAGACTC CTTGCTGTTC 
CTTCTCTGTG CTTATCAGAA TAACAAAGAG CTCTTGTCTA AGGGTCCGTA CAGAGGCCAC 
GATGGGGAGC TGATATCACA TTACAGAAGA GAGTGCTTAC TGAAATTAAA CGAGCAAGCG 
GCCGAGCTGT TTGAGTCTGG AGAGGACGGA GACGTGAACA ATGGCTTAAT TATTATGAAT 
GAGTTTATCG TTCCCTTTCT GCCCTTGTTG CTGGTGGATG ACATGGAAGA GAAAGATATC 
CTTGCTGTGG AAGACATGAG GAATCGCTGG TGTTCATACC TGGGGCAAGA AATGGAAGCA 
AACCTCCAAG AAAAGCTCAC GGATTTTCTG CCAAAACTGC TTGATTGTTC TACAGAGATT 
AAAGGTTTCC ACGAGCCACC AAAGTTACCT TCCTACTCTG CGCACGAACT CTGTGAGCGA 
TTCGCCCGGA TCATGTTGTC CCTCAGTCGT ACGCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu10159
Clone ID Related Accession (Same CDS sequence) NM_013918.2
Accession Version NM_013918.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3168bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-20
Organism Mus musculus(house mouse)
Product ubiquitin carboxyl-terminal hydrolase 25
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048171.1. On Jun 19, 2003 this sequence version replaced NM_013918.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048171.1, AF170563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGGCA 
ATTAAATTAG AGTATTCTAG GCTGGTTAAG TTGGCCCAAG AAGATACTCC ACCAGAAACA 
GATTATCGTT TACATCATGT CCTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATC 
ATTGAGAAAA CATTACTCGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC CCAAGCCAAA CTGGAAATGA TCAAACCTGA AGAAGTGAAC 
TTGGAAGAAT ATGAGGAGTG GCATGCAGAT TATAAGAAAT TCCGAGAAAC AACTATGTAT 
CTCATAACTG GACTAGAAAA CTTTCAGAGA GAAAGTTATA TAGACTCCTT GCTGTTCCTT 
CTCTGTGCTT ATCAGAATAA CAAAGAGCTC TTGTCTAAGG GTCCGTACAG AGGCCACGAT 
GGGGAGCTGA TATCACATTA CAGAAGAGAG TGCTTACTGA AATTAAACGA GCAAGCGGCC 
GAGCTGTTTG AGTCTGGAGA GGACGGAGAC GTGAACAATG GCTTAATTAT TATGAATGAG 
TTTATCGTTC CCTTTCTGCC CTTGTTGCTG GTGGATGACA TGGAAGAGAA AGATATCCTT 
GCTGTGGAAG ACATGAGGAA TCGCTGGTGT TCATACCTGG GGCAAGAAAT GGAAGCAAAC 
CTCCAAGAAA AGCTCACGGA TTTTCTGCCA AAACTGCTTG ATTGTTCTAC AGAGATTAAA 
GGTTTCCACG AGCCACCAAA GTTACCTTCC TACTCTGCGC ACGAACTCTG TGAGCGATTC 
GCCCGGATCA TGTTGTCCCT CAGTCGTACG CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_038946.2
CDS28..3195
Translation

Target ORF information:

RefSeq Version NM_013918.2
Organism Mus musculus(house mouse)
Definition Mus musculus ubiquitin specific peptidase 25 (Usp25), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_013918.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGGCA 
ATTAAATTAG AGTATTCTAG GCTGGTTAAG TTGGCCCAAG AAGATACTCC ACCAGAAACA 
GATTATCGTT TACATCATGT CCTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATC 
ATTGAGAAAA CATTACTCGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC CCAAGCCAAA CTGGAAATGA TCAAACCTGA AGAAGTGAAC 
TTGGAAGAAT ATGAGGAGTG GCATGCAGAT TATAAGAAAT TCCGAGAAAC AACTATGTAT 
CTCATAACTG GACTAGAAAA CTTTCAGAGA GAAAGTTATA TAGACTCCTT GCTGTTCCTT 
CTCTGTGCTT ATCAGAATAA CAAAGAGCTC TTGTCTAAGG GTCCGTACAG AGGCCACGAT 
GGGGAGCTGA TATCACATTA CAGAAGAGAG TGCTTACTGA AATTAAACGA GCAAGCGGCC 
GAGCTGTTTG AGTCTGGAGA GGACGGAGAC GTGAACAATG GCTTAATTAT TATGAATGAG 
TTTATCGTTC CCTTTCTGCC CTTGTTGCTG GTGGATGACA TGGAAGAGAA AGATATCCTT 
GCTGTGGAAG ACATGAGGAA TCGCTGGTGT TCATACCTGG GGCAAGAAAT GGAAGCAAAC 
CTCCAAGAAA AGCTCACGGA TTTTCTGCCA AAACTGCTTG ATTGTTCTAC AGAGATTAAA 
GGTTTCCACG AGCCACCAAA GTTACCTTCC TACTCTGCGC ACGAACTCTG TGAGCGATTC 
GCCCGGATCA TGTTGTCCCT CAGTCGTACG CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu52770
Clone ID Related Accession (Same CDS sequence) XM_006523055.2 , XM_006523055.3
Accession Version XM_006523055.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GCAAGCTTCC 
TTTCAACCCC CGTGGCAGGT CATGATGACC CCCAGTATGC AAGGCGTTAT CATGGCGATA 
GGTAAATCCA GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAATTAGAGT ATTCTAGGCT GGTTAAGTTG GCCCAAGAAG ATACTCCACC AGAAACAGAT 
TATCGTTTAC ATCATGTCCT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATCATT 
GAGAAAACAT TACTCGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCCCA AGCCAAACTG GAAATGATCA AACCTGAAGA AGTGAACTTG 
GAAGAATATG AGGAGTGGCA TGCAGATTAT AAGAAATTCC GAGAAACAAC TATGTATCTC 
ATAACTGGAC TAGAAAACTT TCAGAGAGAA AGTTATATAG ACTCCTTGCT GTTCCTTCTC 
TGTGCTTATC AGAATAACAA AGAGCTCTTG TCTAAGGGTC CGTACAGAGG CCACGATGGG 
GAGCTGATAT CACATTACAG AAGAGAGTGC TTACTGAAAT TAAACGAGCA AGCGGCCGAG 
CTGTTTGAGT CTGGAGAGGA CGGAGACGTG AACAATGGCT TAATTATTAT GAATGAGTTT 
ATCGTTCCCT TTCTGCCCTT GTTGCTGGTG GATGACATGG AAGAGAAAGA TATCCTTGCT 
GTGGAAGACA TGAGGAATCG CTGGTGTTCA TACCTGGGGC AAGAAATGGA AGCAAACCTC 
CAAGAAAAGC TCACGGATTT TCTGCCAAAA CTGCTTGATT GTTCTACAGA GATTAAAGGT 
TTCCACGAGC CACCAAAGTT ACCTTCCTAC TCTGCGCACG AACTCTGTGA GCGATTCGCC 
CGGATCATGT TGTCCCTCAG TCGTACGCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006523118.1
CDS413..3817
Translation

Target ORF information:

RefSeq Version XM_006523055.3
Organism Mus musculus(house mouse)
Definition Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006523055.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GCAAGCTTCC 
TTTCAACCCC CGTGGCAGGT CATGATGACC CCCAGTATGC AAGGCGTTAT CATGGCGATA 
GGTAAATCCA GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAATTAGAGT ATTCTAGGCT GGTTAAGTTG GCCCAAGAAG ATACTCCACC AGAAACAGAT 
TATCGTTTAC ATCATGTCCT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATCATT 
GAGAAAACAT TACTCGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCCCA AGCCAAACTG GAAATGATCA AACCTGAAGA AGTGAACTTG 
GAAGAATATG AGGAGTGGCA TGCAGATTAT AAGAAATTCC GAGAAACAAC TATGTATCTC 
ATAACTGGAC TAGAAAACTT TCAGAGAGAA AGTTATATAG ACTCCTTGCT GTTCCTTCTC 
TGTGCTTATC AGAATAACAA AGAGCTCTTG TCTAAGGGTC CGTACAGAGG CCACGATGGG 
GAGCTGATAT CACATTACAG AAGAGAGTGC TTACTGAAAT TAAACGAGCA AGCGGCCGAG 
CTGTTTGAGT CTGGAGAGGA CGGAGACGTG AACAATGGCT TAATTATTAT GAATGAGTTT 
ATCGTTCCCT TTCTGCCCTT GTTGCTGGTG GATGACATGG AAGAGAAAGA TATCCTTGCT 
GTGGAAGACA TGAGGAATCG CTGGTGTTCA TACCTGGGGC AAGAAATGGA AGCAAACCTC 
CAAGAAAAGC TCACGGATTT TCTGCCAAAA CTGCTTGATT GTTCTACAGA GATTAAAGGT 
TTCCACGAGC CACCAAAGTT ACCTTCCTAC TCTGCGCACG AACTCTGTGA GCGATTCGCC 
CGGATCATGT TGTCCCTCAG TCGTACGCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu52771
Clone ID Related Accession (Same CDS sequence) XM_006523056.2 , XM_006523056.3
Accession Version XM_006523056.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GGTCATGATG 
ACCCCCAGTA TGCAAGGCGT TATCATGGCG ATAGGTAAAT CCAGGAGTGT GTATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAATTAG AGTATTCTAG GCTGGTTAAG 
TTGGCCCAAG AAGATACTCC ACCAGAAACA GATTATCGTT TACATCATGT CCTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATC ATTGAGAAAA CATTACTCGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC CCAAGCCAAA 
CTGGAAATGA TCAAACCTGA AGAAGTGAAC TTGGAAGAAT ATGAGGAGTG GCATGCAGAT 
TATAAGAAAT TCCGAGAAAC AACTATGTAT CTCATAACTG GACTAGAAAA CTTTCAGAGA 
GAAAGTTATA TAGACTCCTT GCTGTTCCTT CTCTGTGCTT ATCAGAATAA CAAAGAGCTC 
TTGTCTAAGG GTCCGTACAG AGGCCACGAT GGGGAGCTGA TATCACATTA CAGAAGAGAG 
TGCTTACTGA AATTAAACGA GCAAGCGGCC GAGCTGTTTG AGTCTGGAGA GGACGGAGAC 
GTGAACAATG GCTTAATTAT TATGAATGAG TTTATCGTTC CCTTTCTGCC CTTGTTGCTG 
GTGGATGACA TGGAAGAGAA AGATATCCTT GCTGTGGAAG ACATGAGGAA TCGCTGGTGT 
TCATACCTGG GGCAAGAAAT GGAAGCAAAC CTCCAAGAAA AGCTCACGGA TTTTCTGCCA 
AAACTGCTTG ATTGTTCTAC AGAGATTAAA GGTTTCCACG AGCCACCAAA GTTACCTTCC 
TACTCTGCGC ACGAACTCTG TGAGCGATTC GCCCGGATCA TGTTGTCCCT CAGTCGTACG 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006523119.1
CDS412..3789
Translation

Target ORF information:

RefSeq Version XM_006523056.3
Organism Mus musculus(house mouse)
Definition Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006523056.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GGTCATGATG 
ACCCCCAGTA TGCAAGGCGT TATCATGGCG ATAGGTAAAT CCAGGAGTGT GTATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAATTAG AGTATTCTAG GCTGGTTAAG 
TTGGCCCAAG AAGATACTCC ACCAGAAACA GATTATCGTT TACATCATGT CCTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATC ATTGAGAAAA CATTACTCGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC CCAAGCCAAA 
CTGGAAATGA TCAAACCTGA AGAAGTGAAC TTGGAAGAAT ATGAGGAGTG GCATGCAGAT 
TATAAGAAAT TCCGAGAAAC AACTATGTAT CTCATAACTG GACTAGAAAA CTTTCAGAGA 
GAAAGTTATA TAGACTCCTT GCTGTTCCTT CTCTGTGCTT ATCAGAATAA CAAAGAGCTC 
TTGTCTAAGG GTCCGTACAG AGGCCACGAT GGGGAGCTGA TATCACATTA CAGAAGAGAG 
TGCTTACTGA AATTAAACGA GCAAGCGGCC GAGCTGTTTG AGTCTGGAGA GGACGGAGAC 
GTGAACAATG GCTTAATTAT TATGAATGAG TTTATCGTTC CCTTTCTGCC CTTGTTGCTG 
GTGGATGACA TGGAAGAGAA AGATATCCTT GCTGTGGAAG ACATGAGGAA TCGCTGGTGT 
TCATACCTGG GGCAAGAAAT GGAAGCAAAC CTCCAAGAAA AGCTCACGGA TTTTCTGCCA 
AAACTGCTTG ATTGTTCTAC AGAGATTAAA GGTTTCCACG AGCCACCAAA GTTACCTTCC 
TACTCTGCGC ACGAACTCTG TGAGCGATTC GCCCGGATCA TGTTGTCCCT CAGTCGTACG 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu52770
Clone ID Related Accession (Same CDS sequence) XM_006523055.2 , XM_006523055.3
Accession Version XM_006523055.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GCAAGCTTCC 
TTTCAACCCC CGTGGCAGGT CATGATGACC CCCAGTATGC AAGGCGTTAT CATGGCGATA 
GGTAAATCCA GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAATTAGAGT ATTCTAGGCT GGTTAAGTTG GCCCAAGAAG ATACTCCACC AGAAACAGAT 
TATCGTTTAC ATCATGTCCT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATCATT 
GAGAAAACAT TACTCGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCCCA AGCCAAACTG GAAATGATCA AACCTGAAGA AGTGAACTTG 
GAAGAATATG AGGAGTGGCA TGCAGATTAT AAGAAATTCC GAGAAACAAC TATGTATCTC 
ATAACTGGAC TAGAAAACTT TCAGAGAGAA AGTTATATAG ACTCCTTGCT GTTCCTTCTC 
TGTGCTTATC AGAATAACAA AGAGCTCTTG TCTAAGGGTC CGTACAGAGG CCACGATGGG 
GAGCTGATAT CACATTACAG AAGAGAGTGC TTACTGAAAT TAAACGAGCA AGCGGCCGAG 
CTGTTTGAGT CTGGAGAGGA CGGAGACGTG AACAATGGCT TAATTATTAT GAATGAGTTT 
ATCGTTCCCT TTCTGCCCTT GTTGCTGGTG GATGACATGG AAGAGAAAGA TATCCTTGCT 
GTGGAAGACA TGAGGAATCG CTGGTGTTCA TACCTGGGGC AAGAAATGGA AGCAAACCTC 
CAAGAAAAGC TCACGGATTT TCTGCCAAAA CTGCTTGATT GTTCTACAGA GATTAAAGGT 
TTCCACGAGC CACCAAAGTT ACCTTCCTAC TCTGCGCACG AACTCTGTGA GCGATTCGCC 
CGGATCATGT TGTCCCTCAG TCGTACGCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006523118.1
CDS414..3818
Translation

Target ORF information:

RefSeq Version XM_006523055.2
Organism Mus musculus(house mouse)
Definition Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006523055.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GCAAGCTTCC 
TTTCAACCCC CGTGGCAGGT CATGATGACC CCCAGTATGC AAGGCGTTAT CATGGCGATA 
GGTAAATCCA GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAATTAGAGT ATTCTAGGCT GGTTAAGTTG GCCCAAGAAG ATACTCCACC AGAAACAGAT 
TATCGTTTAC ATCATGTCCT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATCATT 
GAGAAAACAT TACTCGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCCCA AGCCAAACTG GAAATGATCA AACCTGAAGA AGTGAACTTG 
GAAGAATATG AGGAGTGGCA TGCAGATTAT AAGAAATTCC GAGAAACAAC TATGTATCTC 
ATAACTGGAC TAGAAAACTT TCAGAGAGAA AGTTATATAG ACTCCTTGCT GTTCCTTCTC 
TGTGCTTATC AGAATAACAA AGAGCTCTTG TCTAAGGGTC CGTACAGAGG CCACGATGGG 
GAGCTGATAT CACATTACAG AAGAGAGTGC TTACTGAAAT TAAACGAGCA AGCGGCCGAG 
CTGTTTGAGT CTGGAGAGGA CGGAGACGTG AACAATGGCT TAATTATTAT GAATGAGTTT 
ATCGTTCCCT TTCTGCCCTT GTTGCTGGTG GATGACATGG AAGAGAAAGA TATCCTTGCT 
GTGGAAGACA TGAGGAATCG CTGGTGTTCA TACCTGGGGC AAGAAATGGA AGCAAACCTC 
CAAGAAAAGC TCACGGATTT TCTGCCAAAA CTGCTTGATT GTTCTACAGA GATTAAAGGT 
TTCCACGAGC CACCAAAGTT ACCTTCCTAC TCTGCGCACG AACTCTGTGA GCGATTCGCC 
CGGATCATGT TGTCCCTCAG TCGTACGCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu52771
Clone ID Related Accession (Same CDS sequence) XM_006523056.2 , XM_006523056.3
Accession Version XM_006523056.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GGTCATGATG 
ACCCCCAGTA TGCAAGGCGT TATCATGGCG ATAGGTAAAT CCAGGAGTGT GTATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAATTAG AGTATTCTAG GCTGGTTAAG 
TTGGCCCAAG AAGATACTCC ACCAGAAACA GATTATCGTT TACATCATGT CCTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATC ATTGAGAAAA CATTACTCGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC CCAAGCCAAA 
CTGGAAATGA TCAAACCTGA AGAAGTGAAC TTGGAAGAAT ATGAGGAGTG GCATGCAGAT 
TATAAGAAAT TCCGAGAAAC AACTATGTAT CTCATAACTG GACTAGAAAA CTTTCAGAGA 
GAAAGTTATA TAGACTCCTT GCTGTTCCTT CTCTGTGCTT ATCAGAATAA CAAAGAGCTC 
TTGTCTAAGG GTCCGTACAG AGGCCACGAT GGGGAGCTGA TATCACATTA CAGAAGAGAG 
TGCTTACTGA AATTAAACGA GCAAGCGGCC GAGCTGTTTG AGTCTGGAGA GGACGGAGAC 
GTGAACAATG GCTTAATTAT TATGAATGAG TTTATCGTTC CCTTTCTGCC CTTGTTGCTG 
GTGGATGACA TGGAAGAGAA AGATATCCTT GCTGTGGAAG ACATGAGGAA TCGCTGGTGT 
TCATACCTGG GGCAAGAAAT GGAAGCAAAC CTCCAAGAAA AGCTCACGGA TTTTCTGCCA 
AAACTGCTTG ATTGTTCTAC AGAGATTAAA GGTTTCCACG AGCCACCAAA GTTACCTTCC 
TACTCTGCGC ACGAACTCTG TGAGCGATTC GCCCGGATCA TGTTGTCCCT CAGTCGTACG 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006523119.1
CDS414..3791
Translation

Target ORF information:

RefSeq Version XM_006523056.2
Organism Mus musculus(house mouse)
Definition Mus musculus ubiquitin specific peptidase 25 (Usp25), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006523056.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACATTT 
TTGAATCAGC TGAGAGAAAT TACTGGGATT AATGACGCCC AGATACTCCA ACAAGCCCTG 
AAGGATAGTA ACGGAAACTT GGAATTAGCA GTAGCCTTCC TCACTGCAAA GAATGCCAAG 
ACCCCCCCAC AGGAGGAGAC AGGCTATTAC CAGACAGCGC TTCCAGGCAA CGACAGGTAC 
ATCAGCGTGG GAAGCCAGGC AGATGCAAAT GTAATTGATC TCACTGGAGA TGACAAAGAT 
GATCTTCAGA GAGCGATTGC CTTGAGTTTG GCGGAGTCAA ACAGGGCATT CAGGGAGACG 
GGCATAACCG ATGAAGAGCA GGCCATCAGT AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCATGCT TGAAGAGAAC ACCTATAGAA GTTTGGAGGG ATTCTCGAAA CCCCTATGAC 
AGAAAAAGAC AAGAGAAAGC TCCAGTCGGT CTGAAGAATG TCGGCAACAC CTGCTGGTTT 
AGTGCAGTTA TTCAGTCATT ATTCAATCTT TTGGAGTTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGGG AACTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAGAG GAAGTATGTT 
GATCCGTCCA GAGCAGTTGA GATTCTGAAG GATGCCTTCA AATCCAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAGTTA TTGGACTGGT TGGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAAACGGA TGAAGAGAAG CCAAAGAACC CTATGGTAGA GCTGTTCTAT 
GGAAGATTCC TAGCCATGGG AGTGCTTGAA GGAAAAAAAT TTGAGAACAC TGAAATGTTC 
GGTCAGTATC CACTTCAGGT GAACGGGTTC AAAGACCTGC ATGAGTGCCT GGAGGCCGCC 
ATGATCGAAG GGGAGATCGA ATCCTTACAC TCCGATAACT CAGGAAAGTC AGGCCAGGAG 
CATTGGTTCA CTGAATTGCC GCCGGTATTA ACATTTGAAC TGTCAAGATT TGAATTTAAC 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AATAAACTAG AATTTCCCCA AGTTTTATAT 
CTGGACAGGT ATATGCACAG AAATAGAGAA ATAACAAGAA TTAAACGTGA AGAGATCAAG 
AGACTTAAAG ATTACCTCAC AGTATTACAA CAAAGATTAG AGAGATATTT AAGCTATGGT 
TCCGGTCCCA AAAGATTCCC ATTGGTAGAT GTTCTACAGT ATGCATTGGA GTTTGCCTCC 
AGTAAACCTG TGTGCACTTC CCCCGTTGAT GACATCGATG CTAGTTCCTC AGCAAGTGGC 
CCCCTACCAT CACAGTCCTT ACCAAGCACA ACAGAACAGC AGGGACCCTG TGCTTCAGAT 
CTGCCCGGCT CCTCCTCACC CGCATCAGGT GCCGCCCTGC CCTTGAGGTC AGTGATTCAC 
AAACCCTTCA CTCAGTCTCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATCACAGAGG AAGAACTGTG CGTGCTGGAG AGCTGTTTAC ATCGCTGGAG GACGGAAATA 
GAAAATGACA CGCGAGATCT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAACTC 
ATGTACTCGG ACAAATCCAT GATCCAAGTC CCTTACCGCC TCCATGCCGT CCTAGTTCAT 
GAGGGCCAGG CTAATGCCGG CCACTACTGG GCGTACATCT TTGACCACCG GGAGAGCCGG 
TGGATGAAGT ATAATGATAT TGCTGTGACC AAGTCTTCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTCGGTG GTTATCGAAA TGCCAGTGCA TACTGCTTAA TGTACATAGA CGACAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAGACTGGGC AGGCCCTTGT TGGAATGGAA 
ACCTTACCTC CAGACTTGAG AGACTTTGTT GAGGAGGACA ACCAGCGCTT TGAGAAGGAA 
CTGGAAGAAT GGGACACGCA GCTTGCCCAA CGATCACTGC AAGAAAAGCT GCTAGCTGCG 
CCGAAACTGC GGGAAGCAGA GGCTTCGGCC ACCACAGCAC AAGCAGGAGG CGCAGACTAT 
CTAGAGCAGC CATCAAGAAG TGACTTGTCA AAGCACTGGA AAGAAGAAAC GCTCCGAGTG 
ATTGCCAAGG CGTCACATGA TCTTGAAGAT AAAGGGCCAG AGACAGTTTT GCAGTCGAAA 
CCTGCAAACA CCACGAGCCA ACCACCTTCT AGCCAGCAAG GTGTAGAGGT GGAGATTCCC 
CACTTAGGGA AATTTGTGAT TGAGGCAAAA GAGGGAGGGT ACGATGACGA GGTCATGATG 
ACCCCCAGTA TGCAAGGCGT TATCATGGCG ATAGGTAAAT CCAGGAGTGT GTATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAATTAG AGTATTCTAG GCTGGTTAAG 
TTGGCCCAAG AAGATACTCC ACCAGAAACA GATTATCGTT TACATCATGT CCTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATC ATTGAGAAAA CATTACTCGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC CCAAGCCAAA 
CTGGAAATGA TCAAACCTGA AGAAGTGAAC TTGGAAGAAT ATGAGGAGTG GCATGCAGAT 
TATAAGAAAT TCCGAGAAAC AACTATGTAT CTCATAACTG GACTAGAAAA CTTTCAGAGA 
GAAAGTTATA TAGACTCCTT GCTGTTCCTT CTCTGTGCTT ATCAGAATAA CAAAGAGCTC 
TTGTCTAAGG GTCCGTACAG AGGCCACGAT GGGGAGCTGA TATCACATTA CAGAAGAGAG 
TGCTTACTGA AATTAAACGA GCAAGCGGCC GAGCTGTTTG AGTCTGGAGA GGACGGAGAC 
GTGAACAATG GCTTAATTAT TATGAATGAG TTTATCGTTC CCTTTCTGCC CTTGTTGCTG 
GTGGATGACA TGGAAGAGAA AGATATCCTT GCTGTGGAAG ACATGAGGAA TCGCTGGTGT 
TCATACCTGG GGCAAGAAAT GGAAGCAAAC CTCCAAGAAA AGCTCACGGA TTTTCTGCCA 
AAACTGCTTG ATTGTTCTAC AGAGATTAAA GGTTTCCACG AGCCACCAAA GTTACCTTCC 
TACTCTGCGC ACGAACTCTG TGAGCGATTC GCCCGGATCA TGTTGTCCCT CAGTCGTACG 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.
The Journal of biological chemistry291(25)13206-15(2016 Jun)
Ren Y,Zhao Y,Lin D,Xu X,Zhu Q,Yao J,Shu HB,Zhong B


book

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Proceedings of the National Academy of Sciences of the United States of America113(6)E705-14(2016 Feb)
Jahan AS,Lestra M,Swee LK,Fan Y,Lamers MM,Tafesse FG,Theile CS,Spooner E,Bruzzone R,Ploegh HL,Sanyal S


book

Induction of USP25 by viral infection promotes innate antiviral responses by mediating the stabilization of TRAF3 and TRAF6.
Proceedings of the National Academy of Sciences of the United States of America112(36)11324-9(2015 Sep)
Lin D,Zhang M,Zhang MX,Ren Y,Jin J,Zhao Q,Pan Z,Wu M,Shu HB,Dong C,Zhong B