Fan1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Fan1
Entrez Gene ID	330554
Full Name	FANCD2/FANCI-associated nuclease 1
Synonyms	6030441H18Rik,Mtmr15,mFAN1
General protein information	Preferred Names FANCD2/FANCI-associated nuclease 1 Names fanconi-associated nuclease 1 coiled-coil domain-containing protein MTMR15 myotubularin-related protein 15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540959.3	XP_006541022.1	fanconi-associated nuclease 1 isoform X2
XM_006540959.4	XP_006541022.1	fanconi-associated nuclease 1 isoform X2
XM_006540958.3	XP_006541021.1	fanconi-associated nuclease 1 isoform X1
NM_177893.4	NP_808561.2	fanconi-associated nuclease 1
NM_177893.3	NP_808561.2	fanconi-associated nuclease 1
XM_006540958.4	XP_006541021.1	fanconi-associated nuclease 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FAN1	NP_001158142.1
Rattus norvegicus (Norway rat)	Fan1	NP_001178562.1
Danio rerio (zebrafish)	fan1	NP_001038546.2
Caenorhabditis elegans (roundworm)	fan-1	NP_500997.1
Neurospora crassa	NCU03036	XP_964041.2
Pan troglodytes (chimpanzee)	FAN1	XP_510266.2
Canis lupus familiaris (dog)	FAN1	XP_005618326.1
Gallus gallus (chicken)	FAN1	XP_004943817.1
Homo sapiens (human)	FAN1	NP_055782.3
Magnaporthe oryzae (rice blast fungus)	MGG_00351	XP_003718732.1
Schizosaccharomyces pombe (fission yeast)	SPBC146.06c	NP_595395.1
	FAN1	XP_001109813.1
Mus musculus (house mouse)	Fan1	NP_808561.2
Xenopus tropicalis (tropical clawed frog)	fan1	XP_002934961.2

Gene Ontology
Bibliography

Related articles in PubMed

A FANCD2/FANCI-Associated Nuclease 1-Knockout Model Develops Karyomegalic Interstitial Nephritis.
Airik R, Schueler M, Airik M, Cho J, Porath JD, Mukherjee E, Sims-Lucas S, Hildebrandt F
Journal of the American Society of Nephrology : JASN27(12)3552-3559(2016 Dec)

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Steinmetz CC, Tatavarty V, Sugino K, Shima Y, Joseph A, Lin H, Rutlin M, Lambo M, Hempel CM, Okaty BW, Paradis S, Nelson SB, Turrigiano GG
Cell reports16(10)2711-2722(2016 09)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data show that Fan1 is involved in the physiologic response of kidney tubular cells to DNA damage, which contributes to the pathogenesis of chronic kidney disease. Moreover, Fan1(-/-) mice provide a new model with which to study the pathomechanisms of chronic kidney disease
Title: A FANCD2/FANCI-Associated Nuclease 1-Knockout Model Develops Karyomegalic Interstitial Nephritis.

ubiquitin-binding zinc finger (UBZ) domain of FAN1, which is needed for interaction with FANCD2, is not required for the initial rapid recruitment of FAN1 to ICLs or for its role in DNA ICL resistance.
Title: Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction.

Fan1 nuclease activity promotes ICL repair in a manner that controls ploidy
Title: Karyomegalic interstitial nephritis and DNA damage-induced polyploidy in Fan1 nuclease-defective knock-in mice.

Fan1 recruitment enables processing of stalled forks that is essential for genome stability and health.
Title: Ubiquitinated Fancd2 recruits Fan1 to stalled replication forks to prevent genome instability.

The following Fan1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fan1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38897	XM_006540959.4 Latest version!	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu06998	XM_006540958.4 Latest version!	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu06998	NM_177893.4 Latest version!	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu38897	XM_006540959.3	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu06998	NM_177893.3	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu06998	XM_006540958.3	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fan1 ( XM_006540959.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541022.1 Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu38897
Clone ID Related Accession (Same CDS sequence)	XM_006540959.3 , XM_006540959.4
Accession Version	XM_006540959.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	fanconi-associated nuclease 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540959.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGACGATCCA TGGACAAGAT GCCGCATCTG TCATTCGGAA 
GCTCTTAAAG CTCCCCCATT TCACAAGGAA CGTATTAGAG TTGACAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541022.1
CDS	638..2791
Translation	MPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEDDPWTRCRICHSEALKAPPFHKERIRVDKI

Target ORF information:

RefSeq Version	XM_006540959.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540959.3

ORF Insert Sequence:

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGACGATCCA TGGACAAGAT GCCGCATCTG TCATTCGGAA 
GCTCTTAAAG CTCCCCCATT TCACAAGGAA CGTATTAGAG TTGACAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fan1 ( XM_006540959.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541022.1 Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu38897
Clone ID Related Accession (Same CDS sequence)	XM_006540959.3 , XM_006540959.4
Accession Version	XM_006540959.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	fanconi-associated nuclease 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540959.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGACGATCCA TGGACAAGAT GCCGCATCTG TCATTCGGAA 
GCTCTTAAAG CTCCCCCATT TCACAAGGAA CGTATTAGAG TTGACAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541022.1
CDS	626..2779
Translation	MPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEDDPWTRCRICHSEALKAPPFHKERIRVDKI

Target ORF information:

RefSeq Version	XM_006540959.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540959.4

ORF Insert Sequence:

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGACGATCCA TGGACAAGAT GCCGCATCTG TCATTCGGAA 
GCTCTTAAAG CTCCCCCATT TCACAAGGAA CGTATTAGAG TTGACAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fan1 ( XM_006540958.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541021.1 Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X1, mRNA.

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CloneID	OMu06998
Clone ID Related Accession (Same CDS sequence)	XM_006540958.3 , NM_177893.4 , NM_177893.3 , XM_006540958.4
Accession Version	XM_006540958.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	fanconi-associated nuclease 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540958.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541021.1
CDS	624..3686
Translation	MPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGLG

Target ORF information:

RefSeq Version	XM_006540958.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540958.4

ORF Insert Sequence:

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fan1 ( NM_177893.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808561.2 Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), mRNA.

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CloneID	OMu06998
Clone ID Related Accession (Same CDS sequence)	XM_006540958.3 , NM_177893.4 , NM_177893.3 , XM_006540958.4
Accession Version	NM_177893.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	fanconi-associated nuclease 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129199.3 and AC139849.5. On Jun 17, 2019 this sequence version replaced NM_177893.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808561.2
CDS	374..3436
Translation	MPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGLG

Target ORF information:

RefSeq Version	NM_177893.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177893.4

ORF Insert Sequence:

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fan1 ( NM_177893.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808561.2 Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), mRNA.

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CloneID	OMu06998
Clone ID Related Accession (Same CDS sequence)	XM_006540958.3 , NM_177893.4 , NM_177893.3 , XM_006540958.4
Accession Version	NM_177893.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	fanconi-associated nuclease 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129199.3 and AC139849.5. On or before Sep 10, 2009 this sequence version replaced XM_976717.2, XM_885802.2, NM_177893.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808561.2
CDS	413..3475
Translation	MPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGLG

Target ORF information:

RefSeq Version	NM_177893.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177893.3

ORF Insert Sequence:

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fan1 ( XM_006540958.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541021.1 Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06998
Clone ID Related Accession (Same CDS sequence)	XM_006540958.3 , NM_177893.4 , NM_177893.3 , XM_006540958.4
Accession Version	XM_006540958.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	fanconi-associated nuclease 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540958.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541021.1
CDS	634..3696
Translation	MPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGLG

Target ORF information:

RefSeq Version	XM_006540958.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus FANCD2/FANCI-associated nuclease 1 (Fan1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540958.3

ORF Insert Sequence:

ATGCCGTCAC AAAGGAAATC ACCTGATCAA AAAAGACCCC GTAGAAGCTT ATCTACCAGC 
AAAACTGCAA AAAGCCAGTG TCATTCGATT ACTTCGTATT TTAACAGTGC ACCACCTGCT 
AAACTTGCAT GTTCAACTTG TCATAAAATG GTGCCCAGGT ATGACCTCAT TCGGCACCTT 
GATGAGTCTT GTGCTAACAA CGGTGTTGGT GATGACGTTC AAGTAGAGCC TGCTCAGGCT 
GGATTAATGA GTCCAACTGT GCCTACATCA GATTTACCCA GTGGTCCTTT AGAGAATGTG 
ACACCTCAGA AATTATCACC ACCAAAGAGA AGTTTAATTT CTGTCCAATG TGGTTCAAAA 
CTGGGCATAC AACAGCAGAC CAGTCCCTAC TTTAAAGATG CCTTAGTGTC CAAAGATCAA 
AATGAGCTTC CAAATCAGAG TGTAGAAATT ATGCCTTTGG GAAGTCTGAC ATCGAAATTG 
TCCAGACGAT ACTTAAACGC TAAAAAATCA CTCGCTAAGA ATGAGGGACT AGCCAGTCAG 
TGCCCACAGA CTTCTCCGTC CACACCTGGC ACCAGCCTGA CTGATAACTG TCCAGAGATG 
GAAGACAAAG ATGAGGTTTT GAACAGTTCC CAAAAGGAAA ACATTTATTC ATGTGCACCT 
CTAAAAGAAG AGAATGCTTC AGAACAGAAG GTAAAGAACA ATAAAATAAC GGGAGATGAA 
AGCCAGAAAG CTTCCTGCGG GGAGCCAGCC CTCACCCCTG CATCTGCAGA GCATGCTTCC 
ATATTACTGT CCTCAGATTC AACTCTCGTC AGTAACACGA AATCCTCTCC GGGAGACACT 
CTTGTAAAGC AAGAAAGTGC CAGAAGGGCA GATGTAGGGC TTGCTGAGCC GCTCGAGGTG 
CGCAGTCACA AAGAAGTACA AATGACTTTC GATGCTGCGG CCAAAACGCT GGTGTCTGGG 
GAGGCAGAAT CAAATGGTCC TACCGATGTT GACATGTCTG ACATGACTAC GTGGAGTAAC 
AACCAAGAGC TTGTCAGGGA AGCTGGCAGT GTCTTACACT GCCCTCTGGA GCAGGGGTCC 
AGCTGCGGTG GCCCCAGTGA GACAGCTCAA CTGGCACTGA GTCACCCGTA CTACCTGCGG 
AGCTTCCTGG TGGTGCTGCA GGCCCTCCTT GGGAACGAAG AGGACATGAA GCTCTTCGAT 
GAGCAGGAGA AGGCGATTAT CACCAGGTTT TACCAGTTGT CAGCTAGTGG TCAAAAGTTG 
TATGTGAGGC TCTTTCAACG TAAATTAACC TGGATTAAAA TGAGTAAACT GGAGTATGAA 
GAGATTGCCT CCGACTTAAC CCCTGTGGTT GAAGAATTGA AGGACTCAGG CTTTCTACAG 
ACAGAATCTG AGTTGCAAGA ACTCTCTGAT GTACTTGAAC TCCTTTCTGC TCCTGAGCTG 
AAAGCCCTGG CCAAAACCTT CCACTTGGTG AGTCCCGGTG GGCAGAAGCA GCAGCTGGTA 
GACGCCTTTC ACAAACTGGC CAAACAGCGC TCAGTCTGCA CGTGGGGCAA GACTCAGCCT 
GGAATCCGAG CAGTGATTTT AAAAAGAGCC AAAGACTTGG CTGGCCGGTC GCTTCGAGTC 
TGTAAAGGCC CTAGGGCTGT GTTTGCCCGA ATCTTGCTTC TGTTCTCATT GACTGATTCC 
ATGGAAGACG AAGAAGCTGC TTGTGGGGGT CAAGGTCAGC TGTCTACTGT GCTATTGGTC 
AATCTGGGCC GAATGGAGTT TCCTCAGTAC ACCATCTGCC GGAAGACCCA GATCTTCAGG 
GACAGAGAGG ACCTCATCAG ATACGCAGCA GCCGCACACA TGCTGAGTGA CATCTCAGCT 
GCCATGGCCA GTGGGAACTG GGAGGACGCT AAGGAGCTTG CTCGGAGTGC AAAAAGGGAC 
TGGGAACAAC TGAAAAGCCA CCCTTCCCTG AGGTACCACG AGGCCTTGCC GCCCTTCCTG 
CGCTGCTTTA CCGTCGGGTG GATCTACACA AGGATTTCCT CTCGGGCTGT TGAAGTCCTA 
GAGAGGCTTC ACATGTACGA GGAAGCTGTC AAGGAACTTG AAAACCTCTT GTCTCAGAAA 
ATCTATTGTC CTGACAGCAG AGGCCGCTGG TGGGATCGCC TGGCTCTTAA CCTGCACCAG 
CACCTGAAGC GTCTGGAAGA GGCAATTAGG TGCATCAGGG AAGGCCTGGC AGACCCACAC 
GTGAGGACAG GACACCGACT TTCTCTTTAT CAGCGAGCCG TGCGCCTGCG AGAGTCTCCG 
AGCTGCAGGA AGTACAAGCA CCTCTTCAGC CGGCTGCCCG AGGTTGCCGT GGGAGACGTC 
AAGCATGTGA CCATCACGGG CAGGCTGTGC CCACAGCATG GCATGGGCAA GTCTGTGTTT 
GTCATGGAGA GTGGAGACGG CGCCAACCCC ACCACAGTCC TGTGTTCTGT GGAGGAGCTG 
GCCCTGGGTT ATTACAGGCA GAGTGGCTTT GACCAAGGGA TTCATGGAGA AGGGTCCACC 
TTCAGCACCC TGTGTGGCCT CCTGCTGTGG GATATCATCT TCATGGATGG AATACCAGAC 
GTCTTCAGAA ATGCCTACCA GGCATCCCCA CTGGATTTGC TCACGGACAG CTTCTTCGCA 
AGCAGGGAGC AGGCCCTGGA AGCCAGGCTG CAGCTGATCC ACAGCGCCCC TGCTGAGAGC 
CTGCGGGCCT GGGTGGGCGA AGCATGGCAG GCCCAGCAAG GCAGAGTGGC CTCTCTGGTC 
AGCTGGGACC GCTTCACCTC CCTACAGCAA GCTCAGGATC TTGTCTCCTG CCTCGGGGGT 
CCTGTCCTCA GTGGTGTGTG CAGGCGCCTG GCTGCTGACT TTCGGCACTG CCGAGGGGGC 
CTCCCAGACT TGGTGGTGTG GAATTCTCAG AGCCACCATT GCAAGCTGGT GGAGGTGAAA 
GGCCCCAGTG ATCGACTTTC ATGTAAGCAG ATGATCTGGC TGTACGAACT CCAAAAGCTG 
GGGGCTGATG TAGAAGTGTG CCATGTGGTT GCAGTTGGTG CTAAGAGCAA AGGTCTTGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A FANCD2/FANCI-Associated Nuclease 1-Knockout Model Develops Karyomegalic Interstitial Nephritis.
Journal of the American Society of Nephrology : JASN27(12)3552-3559(2016 Dec)
Airik R,Schueler M,Airik M,Cho J,Porath JD,Mukherjee E,Sims-Lucas S,Hildebrandt F

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Cell reports16(10)2711-2722(2016 09)
Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Fan1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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