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Hcn4 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Hcn4
Entrez Gene ID 330953
Full Name hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Synonyms Bcng3,Hcn3
General protein information
Preferred Names

hyperpolarization-activated, cyclic nucleotide-gated K+ 4

Names

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
BCNG-3
brain cyclic nucleotide-gated channel 3
hyperpolarization-activated, cyclic nucleotide-gated K+ 3
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

9

9|9 B

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001081192.1 NP_001074661.1 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
NM_001081192.2 NP_001074661.1 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
NM_001081192.3 NP_001074661.1 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Pathways from BioSystems


Homologies


HCN4 XP_002804905.1
Mus musculus (house mouse) Hcn4 NP_001074661.1
Danio rerio (zebrafish) hcn4 XP_686078.4
Xenopus tropicalis (tropical clawed frog) hcn4 XP_004916024.1
Pan troglodytes (chimpanzee) HCN4 XP_003952750.1
Bos taurus (cattle) HCN4 XP_002690539.1
Gallus gallus (chicken) HCN4 XP_425050.3
Homo sapiens (human) HCN4 NP_005468.1
Danio rerio (zebrafish) hcn4l XP_684730.5
Rattus norvegicus (Norway rat) Hcn4 NP_067690.1

Gene Ontology

The following Hcn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu21744 NM_001081192.3
Latest version!		 Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $706.30
$1009.00
View Old Accession Versions >>
OMu21744 NM_001081192.1 Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $706.30
$1009.00
OMu21744 NM_001081192.2 Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $706.30
$1009.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu21744
Clone ID Related Accession (Same CDS sequence) NM_001081192.1 , NM_001081192.2 , NM_001081192.3
Accession Version NM_001081192.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-30
Organism Mus musculus(house mouse)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC110530.10. On Feb 6, 2007 this sequence version replaced XM_287905.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC 
CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG 
GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG 
GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG 
GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG 
CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC 
GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA 
CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG 
CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC 
CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC 
ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG 
GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC 
TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA 
CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG 
GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG 
GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC 
TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG 
ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT 
AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG 
TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC 
CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC 
ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC 
TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG 
TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC 
GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA 
GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC 
CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA 
GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG 
ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT 
CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT 
TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC 
AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG 
ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC 
CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG 
AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC 
CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG 
ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG 
GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG 
CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC 
CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA 
CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT 
TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA 
CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT 
TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC 
GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA 
TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC 
CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG 
CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT 
GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG 
CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC 
CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT 
GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT 
CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA 
CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT 
CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC 
AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT 
GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC 
CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT 
GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001074661.1
CDS1..3606
Translation

Target ORF information:

RefSeq Version NM_001081192.1
Organism Mus musculus(house mouse)
Definition Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001081192.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC 
CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG 
GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG 
GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG 
GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG 
CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC 
GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA 
CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG 
CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC 
CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC 
ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG 
GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC 
TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA 
CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG 
GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG 
GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC 
TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG 
ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT 
AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG 
TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC 
CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC 
ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC 
TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG 
TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC 
GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA 
GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC 
CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA 
GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG 
ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT 
CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT 
TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC 
AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG 
ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC 
CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG 
AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC 
CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG 
ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG 
GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG 
CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC 
CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA 
CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT 
TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA 
CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT 
TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC 
GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA 
TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC 
CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG 
CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT 
GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG 
CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC 
CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT 
GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT 
CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA 
CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT 
CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC 
AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT 
GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC 
CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT 
GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu21744
Clone ID Related Accession (Same CDS sequence) NM_001081192.1 , NM_001081192.2 , NM_001081192.3
Accession Version NM_001081192.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-27
Organism Mus musculus(house mouse)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110530.10. On Jun 21, 2018 this sequence version replaced NM_001081192.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC 
CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG 
GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG 
GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG 
GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG 
CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC 
GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA 
CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG 
CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC 
CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC 
ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG 
GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC 
TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA 
CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG 
GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG 
GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC 
TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG 
ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT 
AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG 
TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC 
CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC 
ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC 
TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG 
TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC 
GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA 
GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC 
CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA 
GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG 
ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT 
CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT 
TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC 
AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG 
ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC 
CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG 
AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC 
CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG 
ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG 
GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG 
CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC 
CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA 
CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT 
TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA 
CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT 
TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC 
GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA 
TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC 
CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG 
CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT 
GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG 
CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC 
CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT 
GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT 
CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA 
CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT 
CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC 
AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT 
GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC 
CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT 
GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001074661.1
CDS658..4263
Translation

Target ORF information:

RefSeq Version NM_001081192.2
Organism Mus musculus(house mouse)
Definition Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001081192.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC 
CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG 
GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG 
GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG 
GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG 
CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC 
GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA 
CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG 
CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC 
CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC 
ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG 
GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC 
TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA 
CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG 
GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG 
GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC 
TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG 
ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT 
AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG 
TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC 
CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC 
ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC 
TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG 
TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC 
GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA 
GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC 
CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA 
GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG 
ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT 
CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT 
TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC 
AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG 
ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC 
CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG 
AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC 
CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG 
ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG 
GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG 
CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC 
CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA 
CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT 
TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA 
CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT 
TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC 
GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA 
TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC 
CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG 
CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT 
GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG 
CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC 
CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT 
GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT 
CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA 
CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT 
CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC 
AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT 
GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC 
CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT 
GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu21744
Clone ID Related Accession (Same CDS sequence) NM_001081192.1 , NM_001081192.2 , NM_001081192.3
Accession Version NM_001081192.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Mus musculus(house mouse)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110530.10. On Jun 18, 2019 this sequence version replaced NM_001081192.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158106.1, SRR7345562.1266554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC 
CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG 
GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG 
GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG 
GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG 
CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC 
GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA 
CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG 
CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC 
CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC 
ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG 
GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC 
TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA 
CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG 
GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG 
GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC 
TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG 
ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT 
AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG 
TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC 
CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC 
ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC 
TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG 
TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC 
GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA 
GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC 
CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA 
GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG 
ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT 
CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT 
TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC 
AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG 
ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC 
CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG 
AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC 
CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG 
ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG 
GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG 
CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC 
CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA 
CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT 
TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA 
CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT 
TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC 
GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA 
TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC 
CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG 
CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT 
GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG 
CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC 
CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT 
GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT 
CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA 
CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT 
CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC 
AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT 
GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC 
CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT 
GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001074661.1
CDS564..4169
Translation

Target ORF information:

RefSeq Version NM_001081192.3
Organism Mus musculus(house mouse)
Definition Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001081192.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC 
CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG 
GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG 
GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG 
GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG 
CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC 
GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA 
CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG 
CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC 
CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC 
ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG 
GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC 
TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA 
CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG 
GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG 
GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC 
TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG 
ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT 
AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG 
TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC 
CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC 
ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC 
TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG 
TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC 
GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA 
GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC 
CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA 
GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG 
ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT 
CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT 
TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC 
AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG 
ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC 
CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG 
AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC 
CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG 
ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG 
GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG 
CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC 
CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA 
CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT 
TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA 
CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT 
TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC 
GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA 
TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC 
CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG 
CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT 
GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG 
CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC 
CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT 
GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT 
CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA 
CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT 
CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC 
AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT 
GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC 
CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT 
GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT 
TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed