Ripply2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Ripply2
Entrez Gene ID	382089
Full Name	ripply transcriptional repressor 2
Synonyms	C030002E08Rik,Gm1122
General protein information	Preferred Names ripply transcriptional repressor 2 Names protein ripply2 ripply2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511261.4	XP_006511324.3	protein ripply2 isoform X1
NM_001037907.1	NP_001032996.1	protein ripply2
XM_006511261.3	XP_006511324.2	protein ripply2 isoform X1
XM_030244437.1	XP_030100297.1	protein ripply2 isoform X3
XM_030244436.1	XP_030100296.1	protein ripply2 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RIPPLY2	NP_001009994.1
Pan troglodytes (chimpanzee)	RIPPLY2	XP_003311415.1
Rattus norvegicus (Norway rat)	Ripply2	XP_343445.2
Bos taurus (cattle)	LOC781965	XP_002690259.2
	RIPPLY2	XP_001085600.1
Mus musculus (house mouse)	Ripply2	NP_001032996.1

Gene Ontology
Bibliography

Related articles in PubMed

Supt20 is required for development of the axial skeleton.
Warrier S, Nuwayhid S, Sabatino JA, Sugrue KF, Zohn IE
Developmental biology421(2)245-257(2017 Jan)

Hoxb6 can interfere with somitogenesis in the posterior embryo through a mechanism independent of its rib-promoting activity.
Casaca A, N?voa A, Mallo M
Development (Cambridge, England)143(3)437-48(2016 Feb)

Segmental border is defined by Ripply2-mediated Tbx6 repression independent of Mesp2.
Zhao W, Ajima R, Ninomiya Y, Saga Y
Developmental biology400(1)105-17(2015 Apr)

Compound heterozygous mutations in RIPPLY2 associated with vertebral segmentation defects.
McInerney-Leo AM, Sparrow DB, Harris JE, Gardiner BB, Marshall MS, O'Reilly VC, Shi H, Brown MA, Leo PJ, Zankl A, Dunwoodie SL, Duncan EL
Human molecular genetics24(5)1234-42(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ripply2 represses Tbx6 in a Mesp2-independent manner, which contributes to the accurate segmental border formation.
Title: Segmental border is defined by Ripply2-mediated Tbx6 repression independent of Mesp2.

Compound heterozygous mutations in RIPPLY2 are associated with segmentation defects of the vertebrae, a new gene for this condition.
Title: Compound heterozygous mutations in RIPPLY2 associated with vertebral segmentation defects.

conclusion was supported by analyses of Mesp2 KO and Ripply1/2 double KO embryos lacking rostral and caudal properties, respectively
Title: Metameric pattern of intervertebral disc/vertebral body is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somites in the mouse embryo.

Rostro-caudal pattern is established by dynamic interaction of Notch activity with two Mesp2 domains, which are defined in successive segmentation cycles by Notch, Tbx6 and Ripply1/2.
Title: Analysis of Ripply1/2-deficient mouse embryos reveals a mechanism underlying the rostro-caudal patterning within a somite.

findings show that Ripply2 is expressed in the anterior presomitic mesoderm and that it lies downstream of Wnt3a; data are consistent with Ripply2 functioning as a segment boundary determination gene during mammalian embryogenesis
Title: Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis.

The following Ripply2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ripply2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00127	NM_001037907.1 Latest version!	Mus musculus ripply transcriptional repressor 2 (Ripply2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu42855	XM_006511261.4 Latest version!	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu82876	XM_006511261.3 Latest version!	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu90213	XM_030244436.1 Latest version!	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu90214	XM_030244437.1 Latest version!	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ripply2 ( NM_001037907.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001032996.1 Mus musculus ripply transcriptional repressor 2 (Ripply2), mRNA.

Price & Availability↑

CloneID	OMu00127
Clone ID Related Accession (Same CDS sequence)	NM_001037907.1
Accession Version	NM_001037907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-06
Organism	Mus musculus(house mouse)
Product	protein ripply2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB212223.1 and BB354769.1. On or before Jul 25, 2007 this sequence version replaced XM_918212.3, XM_985855.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB212223.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849379, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATACCA CCGAGAGCGC CGAGAGCGCG CACAACCCAG CACGTCCGCC CTCGCGCAGC 
CGCTGCCCGC CGTCCGCGCA GCCCGGGTCC GAGGGCTTCT GGAGACCGTG GGTTCGAACT 
CCTGGAGAGA AAGAGAAGCG GACGGGGCCC CGTGCTGCAG AGGCGTTGCC CAGTGGCCCA 
GGAATGGCAG AAGCTTCCGG GAAGCTTTTG CAATACCAAC ACCCTGTCAG GCTATTCTGG 
CCCAAGTCCA AGTGTTACGA CTACTTATAT CAAGAAGCAG AAACTCTTCT GAAAAATTTC 
CCAATTCAAG CTACAATTTC GTTTTATGAA GATTCTGATA GTGAAGATGA AATAGAGGGG 
CTGGCCTGTG AAAATCAATC TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032996.1
CDS	9..395
Translation	MDTTESAESAHNPARPPSRSRCPPSAQPGSEGFWRPWVRTPGEKEKRTGPRAAEALPSGPGMAEASGKLLQYQHPVRLFWPKSKCYDYLYQEAETLLKNFPIQATISFYEDSDSEDEIEGLACENQSN

Target ORF information:

RefSeq Version	NM_001037907.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ripply transcriptional repressor 2 (Ripply2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037907.1

ORF Insert Sequence:

ATGGATACCA CCGAGAGCGC CGAGAGCGCG CACAACCCAG CACGTCCGCC CTCGCGCAGC 
CGCTGCCCGC CGTCCGCGCA GCCCGGGTCC GAGGGCTTCT GGAGACCGTG GGTTCGAACT 
CCTGGAGAGA AAGAGAAGCG GACGGGGCCC CGTGCTGCAG AGGCGTTGCC CAGTGGCCCA 
GGAATGGCAG AAGCTTCCGG GAAGCTTTTG CAATACCAAC ACCCTGTCAG GCTATTCTGG 
CCCAAGTCCA AGTGTTACGA CTACTTATAT CAAGAAGCAG AAACTCTTCT GAAAAATTTC 
CCAATTCAAG CTACAATTTC GTTTTATGAA GATTCTGATA GTGAAGATGA AATAGAGGGG 
CTGGCCTGTG AAAATCAATC TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ripply2 ( XM_006511261.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511324.3 Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42855
Clone ID Related Accession (Same CDS sequence)	XM_006511261.4
Accession Version	XM_006511261.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein ripply2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511261.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTT TCTCCCAGAC CGTGTGTCAG GGGGCTATTA ACACTCGCTC CAGCAGCCGG 
TTCCCGGGCC ATTTGGGATG TTGCACGCGT TCACACCCGC GCGCGGCCCG CGGCGCCTTC 
ACGCGCTGCC GTTTTGTTTA TGCAGAGCAG CGAGGGCGAC CGGGGTGCGA AGGTCTGCAA 
GGGCTAGATA AAGCCGGGCT GCGACACCGA CGCGGTCGTC CTGCCATGGA TACCACCGAG 
AGCGCCGAGA GCGCGCACAA CCCAGCACGT CCGCCCTCGC GCAGCCGCTG CCCGCCGTCC 
GCGCAGCCCG GGTACCTATC AGCCGGGATC CATCCCCGCG CATTCTCCAC CCTCGCCCCG 
TGCCCGCCGC CCACTGAGCC CGAGGACCGC CTGGCCCCGG CTCGTCCCTG TTCGCCCTCT 
ACTTCAGCCC TCGCCTTTTA CCCGCCGCTC TTGCCTTTGC CCGGGCCCGG TGCCCTTACT 
AAGTCCGAGT TGAGTCCGGT GGCTGGGCTC ACGTTCTTTG TGCGCGACTT GCGCTTGCCA 
TCCTGCAGGT CCGAGGGCTT CTGGAGACCG TGGGTTCGAA CTCCTGGAGA GAAAGAGAAG 
CGGACGGGGC CCCGTGCTGC AGAGGCGTTG CCCAGTGGCC CAGGAATGGC AGAAGCTTCC 
GGGAAGCTTT TGCAATACCA ACACCCTGTC AGGCTATTCT GGCCCAAGTC CAAGTGTTAC 
GACTACTTAT ATCAAGAAGC AGAAACTCTT CTGAAAAATT TCCCAATTCA AGCTACAATT 
TCGTTTTATG AAGATTCTGA TAGTGAAGAT GAAATAGAGG GGCTGGCCTG TGAAAATCAA 
TCTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511324.3
CDS	10..858
Translation	MLFFSQTVCQGAINTRSSSRFPGHLGCCTRSHPRAARGAFTRCRFVYAEQRGRPGCEGLQGLDKAGLRHRRGRPAMDTTESAESAHNPARPPSRSRCPPSAQPGYLSAGIHPRAFSTLAPCPPPTEPEDRLAPARPCSPSTSALAFYPPLLPLPGPGALTKSELSPVAGLTFFVRDLRLPSCRSEGFWRPWVRTPGEKEKRTGPRAAEALPSGPGMAEASGKLLQYQHPVRLFWPKSKCYDYLYQEAETLLKNFPIQATISFYEDSDSEDEIEGLACENQSN

Target ORF information:

RefSeq Version	XM_006511261.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511261.4

ORF Insert Sequence:

ATGCTGTTTT TCTCCCAGAC CGTGTGTCAG GGGGCTATTA ACACTCGCTC CAGCAGCCGG 
TTCCCGGGCC ATTTGGGATG TTGCACGCGT TCACACCCGC GCGCGGCCCG CGGCGCCTTC 
ACGCGCTGCC GTTTTGTTTA TGCAGAGCAG CGAGGGCGAC CGGGGTGCGA AGGTCTGCAA 
GGGCTAGATA AAGCCGGGCT GCGACACCGA CGCGGTCGTC CTGCCATGGA TACCACCGAG 
AGCGCCGAGA GCGCGCACAA CCCAGCACGT CCGCCCTCGC GCAGCCGCTG CCCGCCGTCC 
GCGCAGCCCG GGTACCTATC AGCCGGGATC CATCCCCGCG CATTCTCCAC CCTCGCCCCG 
TGCCCGCCGC CCACTGAGCC CGAGGACCGC CTGGCCCCGG CTCGTCCCTG TTCGCCCTCT 
ACTTCAGCCC TCGCCTTTTA CCCGCCGCTC TTGCCTTTGC CCGGGCCCGG TGCCCTTACT 
AAGTCCGAGT TGAGTCCGGT GGCTGGGCTC ACGTTCTTTG TGCGCGACTT GCGCTTGCCA 
TCCTGCAGGT CCGAGGGCTT CTGGAGACCG TGGGTTCGAA CTCCTGGAGA GAAAGAGAAG 
CGGACGGGGC CCCGTGCTGC AGAGGCGTTG CCCAGTGGCC CAGGAATGGC AGAAGCTTCC 
GGGAAGCTTT TGCAATACCA ACACCCTGTC AGGCTATTCT GGCCCAAGTC CAAGTGTTAC 
GACTACTTAT ATCAAGAAGC AGAAACTCTT CTGAAAAATT TCCCAATTCA AGCTACAATT 
TCGTTTTATG AAGATTCTGA TAGTGAAGAT GAAATAGAGG GGCTGGCCTG TGAAAATCAA 
TCTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ripply2 ( XM_006511261.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511324.2 Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82876
Clone ID Related Accession (Same CDS sequence)	XM_006511261.3
Accession Version	XM_006511261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein ripply2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCA CCGAGAGCGC CGAGAGCGCG CACAACCCAG CACGTCCGCC CTCGCGCAGC 
CGCTGCCCGC CGTCCGCGCA GCCCGGGTAC CTATCAGCCG GGATCCATCC CCGCGCATTC 
TCCACCCTCG CCCCGTGCCC GCCGCCCACT GAGCCCGAGG ACCGCCTGGC CCCGGCTCGT 
CCCTGTTCGC CCTCTACTTC AGCCCTCGCC TTTTACCCGC CGCTCTTGCC TTTGCCCGGG 
CCCGGTGCCC TTACTAAGTC CGAGTTGAGT CCGGTGGCTG GGCTCACGTT CTTTGTGCGC 
GACTTGCGCT TGCCATCCTG CAGGTCCGAG GGCTTCTGGA GACCGTGGGT TCGAACTCCT 
GGAGAGAAAG AGAAGCGGAC GGGGCCCCGT GCTGCAGAGG CGTTGCCCAG TGGCCCAGGA 
ATGGCAGAAG CTTCCGGGAA GCTTTTGCAA TACCAACACC CTGTCAGGCT ATTCTGGCCC 
AAGTCCAAGT GTTACGACTA CTTATATCAA GAAGCAGAAA CTCTTCTGAA AAATTTCCCA 
ATTCAAGCTA CAATTTCGTT TTATGAAGAT TCTGATAGTG AAGATGAAAT AGAGGGGCTG 
GCCTGTGAAA ATCAATCTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511324.2
CDS	170..793
Translation	MDTTESAESAHNPARPPSRSRCPPSAQPGYLSAGIHPRAFSTLAPCPPPTEPEDRLAPARPCSPSTSALAFYPPLLPLPGPGALTKSELSPVAGLTFFVRDLRLPSCRSEGFWRPWVRTPGEKEKRTGPRAAEALPSGPGMAEASGKLLQYQHPVRLFWPKSKCYDYLYQEAETLLKNFPIQATISFYEDSDSEDEIEGLACENQSN

Target ORF information:

RefSeq Version	XM_006511261.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511261.3

ORF Insert Sequence:

ATGGATACCA CCGAGAGCGC CGAGAGCGCG CACAACCCAG CACGTCCGCC CTCGCGCAGC 
CGCTGCCCGC CGTCCGCGCA GCCCGGGTAC CTATCAGCCG GGATCCATCC CCGCGCATTC 
TCCACCCTCG CCCCGTGCCC GCCGCCCACT GAGCCCGAGG ACCGCCTGGC CCCGGCTCGT 
CCCTGTTCGC CCTCTACTTC AGCCCTCGCC TTTTACCCGC CGCTCTTGCC TTTGCCCGGG 
CCCGGTGCCC TTACTAAGTC CGAGTTGAGT CCGGTGGCTG GGCTCACGTT CTTTGTGCGC 
GACTTGCGCT TGCCATCCTG CAGGTCCGAG GGCTTCTGGA GACCGTGGGT TCGAACTCCT 
GGAGAGAAAG AGAAGCGGAC GGGGCCCCGT GCTGCAGAGG CGTTGCCCAG TGGCCCAGGA 
ATGGCAGAAG CTTCCGGGAA GCTTTTGCAA TACCAACACC CTGTCAGGCT ATTCTGGCCC 
AAGTCCAAGT GTTACGACTA CTTATATCAA GAAGCAGAAA CTCTTCTGAA AAATTTCCCA 
ATTCAAGCTA CAATTTCGTT TTATGAAGAT TCTGATAGTG AAGATGAAAT AGAGGGGCTG 
GCCTGTGAAA ATCAATCTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ripply2 ( XM_030244436.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100296.1 Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu90213
Clone ID Related Accession (Same CDS sequence)	XM_030244436.1
Accession Version	XM_030244436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein ripply2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTT TCTCCCAGAC CGTGTGTCAG GGGGCTATTA ACACTCGCTC CAGCAGCCGG 
TTCCCGGGCC ATTTGGGATG TTGCACGCGT TCACACCCGC GCGCGGCCCG CGGCGCCTTC 
ACGCGCTGCC GTTTTGTTTA TGCAGAGCAG CGAGGGCGAC CGGGGTGCGA AGGTCTGCAA 
GGGCTAGATA AAGCCGGGCT GCGACACCGA CGCGGTCGTC CTGCCATGGA TACCACCGAG 
AGCGCCGAGA GCGCGCACAA CCCAGCACGT CCGCCCTCGC GCAGCCGCTG CCCGCCGTCC 
GCGCAGCCCG GGTACCTATC AGCCGGGATC CATCCCCGCG CATTCTCCAC CCTCGCCCCG 
TGCCCGCCGC CCACTGAGCC CGAGGACCGC CTGGCCCCGG CTCGTCCCTG TTCGCCCTCT 
ACTTCAGCCC TCGCCTTTTA CCCGCCGCTC TTGCCTTTGC CCGGGCCCGG TGCCCTTACT 
AAGTCCGAGT TGAGTCCGGT GGCTGGGCTC ACGTTCTTTG TGCGCGACTT GCGCTTGCCA 
TCCTGCAGGT CCGAGGGCTT CTGGAGACCG TGGGTTCGAA CTCCTGGAGA GAAAGAGAAG 
CGGACGGGGC CCCGTGCTGC AGAGGCGGCT ATTCTGGCCC AAGTCCAAGT GTTACGACTA 
CTTATATCAA GAAGCAGAAA CTCTTCTGAA AAATTTCCCA ATTCAAGCTA CAATTTCGTT 
TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100296.1
CDS	10..735
Translation	MLFFSQTVCQGAINTRSSSRFPGHLGCCTRSHPRAARGAFTRCRFVYAEQRGRPGCEGLQGLDKAGLRHRRGRPAMDTTESAESAHNPARPPSRSRCPPSAQPGYLSAGIHPRAFSTLAPCPPPTEPEDRLAPARPCSPSTSALAFYPPLLPLPGPGALTKSELSPVAGLTFFVRDLRLPSCRSEGFWRPWVRTPGEKEKRTGPRAAEAAILAQVQVLRLLISRSRNSSEKFPNSSYNFVL

Target ORF information:

RefSeq Version	XM_030244436.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244436.1

ORF Insert Sequence:

ATGCTGTTTT TCTCCCAGAC CGTGTGTCAG GGGGCTATTA ACACTCGCTC CAGCAGCCGG 
TTCCCGGGCC ATTTGGGATG TTGCACGCGT TCACACCCGC GCGCGGCCCG CGGCGCCTTC 
ACGCGCTGCC GTTTTGTTTA TGCAGAGCAG CGAGGGCGAC CGGGGTGCGA AGGTCTGCAA 
GGGCTAGATA AAGCCGGGCT GCGACACCGA CGCGGTCGTC CTGCCATGGA TACCACCGAG 
AGCGCCGAGA GCGCGCACAA CCCAGCACGT CCGCCCTCGC GCAGCCGCTG CCCGCCGTCC 
GCGCAGCCCG GGTACCTATC AGCCGGGATC CATCCCCGCG CATTCTCCAC CCTCGCCCCG 
TGCCCGCCGC CCACTGAGCC CGAGGACCGC CTGGCCCCGG CTCGTCCCTG TTCGCCCTCT 
ACTTCAGCCC TCGCCTTTTA CCCGCCGCTC TTGCCTTTGC CCGGGCCCGG TGCCCTTACT 
AAGTCCGAGT TGAGTCCGGT GGCTGGGCTC ACGTTCTTTG TGCGCGACTT GCGCTTGCCA 
TCCTGCAGGT CCGAGGGCTT CTGGAGACCG TGGGTTCGAA CTCCTGGAGA GAAAGAGAAG 
CGGACGGGGC CCCGTGCTGC AGAGGCGGCT ATTCTGGCCC AAGTCCAAGT GTTACGACTA 
CTTATATCAA GAAGCAGAAA CTCTTCTGAA AAATTTCCCA ATTCAAGCTA CAATTTCGTT 
TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ripply2 ( XM_030244437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100297.1 Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu90214
Clone ID Related Accession (Same CDS sequence)	XM_030244437.1
Accession Version	XM_030244437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein ripply2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTT TCTCCCAGAC CGTGTGTCAG GGGGCTATTA ACACTCGCTC CAGCAGCCGG 
TTCCCGGGCC ATTTGGGATG TTGCACGCGT TCACACCCGC GCGCGGCCCG CGGCGCCTTC 
ACGCGCTGCC GTTTTGTTTA TGCAGAGCAG CGAGGGCGAC CGGGGTGCGA AGGTCTGCAA 
GGGCTAGATA AAGCCGGGCT GCGACACCGA CGCGGTCGTC CTGCCATGGA TACCACCGAG 
AGCGCCGAGA GCGCGCACAA CCCAGCACGT CCGCCCTCGC GCAGCCGCTG CCCGCCGTCC 
GCGCAGCCCG GGTCCGAGGG CTTCTGGAGA CCGTGGGTTC GAACTCCTGG AGAGAAAGAG 
AAGCGGACGG GGCCCCGTGC TGCAGAGGCG GCTATTCTGG CCCAAGTCCA AGTGTTACGA 
CTACTTATAT CAAGAAGCAG AAACTCTTCT GAAAAATTTC CCAATTCAAG CTACAATTTC 
GTTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100297.1
CDS	12..500
Translation	MLFFSQTVCQGAINTRSSSRFPGHLGCCTRSHPRAARGAFTRCRFVYAEQRGRPGCEGLQGLDKAGLRHRRGRPAMDTTESAESAHNPARPPSRSRCPPSAQPGSEGFWRPWVRTPGEKEKRTGPRAAEAAILAQVQVLRLLISRSRNSSEKFPNSSYNFVL

Target ORF information:

RefSeq Version	XM_030244437.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ripply transcriptional repressor 2 (Ripply2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244437.1

ORF Insert Sequence:

ATGCTGTTTT TCTCCCAGAC CGTGTGTCAG GGGGCTATTA ACACTCGCTC CAGCAGCCGG 
TTCCCGGGCC ATTTGGGATG TTGCACGCGT TCACACCCGC GCGCGGCCCG CGGCGCCTTC 
ACGCGCTGCC GTTTTGTTTA TGCAGAGCAG CGAGGGCGAC CGGGGTGCGA AGGTCTGCAA 
GGGCTAGATA AAGCCGGGCT GCGACACCGA CGCGGTCGTC CTGCCATGGA TACCACCGAG 
AGCGCCGAGA GCGCGCACAA CCCAGCACGT CCGCCCTCGC GCAGCCGCTG CCCGCCGTCC 
GCGCAGCCCG GGTCCGAGGG CTTCTGGAGA CCGTGGGTTC GAACTCCTGG AGAGAAAGAG 
AAGCGGACGG GGCCCCGTGC TGCAGAGGCG GCTATTCTGG CCCAAGTCCA AGTGTTACGA 
CTACTTATAT CAAGAAGCAG AAACTCTTCT GAAAAATTTC CCAATTCAAG CTACAATTTC 
GTTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Supt20 is required for development of the axial skeleton.
Developmental biology421(2)245-257(2017 Jan)
Warrier S,Nuwayhid S,Sabatino JA,Sugrue KF,Zohn IE

Hoxb6 can interfere with somitogenesis in the posterior embryo through a mechanism independent of its rib-promoting activity.
Development (Cambridge, England)143(3)437-48(2016 Feb)
Casaca A,N?voa A,Mallo M

Segmental border is defined by Ripply2-mediated Tbx6 repression independent of Mesp2.
Developmental biology400(1)105-17(2015 Apr)
Zhao W,Ajima R,Ninomiya Y,Saga Y

Compound heterozygous mutations in RIPPLY2 associated with vertebral segmentation defects.
Human molecular genetics24(5)1234-42(2015 Mar)
McInerney-Leo AM,Sparrow DB,Harris JE,Gardiner BB,Marshall MS,O'Reilly VC,Shi H,Brown MA,Leo PJ,Zankl A,Dunwoodie SL,Duncan EL

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Ripply2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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