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Ddx20 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Ddx20
Entrez Gene ID 53975
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Synonyms GEMIN3,dp103
General protein information
Preferred Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20

Names

probable ATP-dependent RNA helicase DDX20
DEAD box protein 20
DEAD box protein DP 103
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20
ROSF-1
component of gems 3
gemin-3
regulator of steroidogenic factor 1
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

3

3|3 F2.2

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_017397.3 NP_059093.3 probable ATP-dependent RNA helicase DDX20
XM_006501701.3 XP_006501764.1 probable ATP-dependent RNA helicase DDX20 isoform X1
XM_006501701.2 XP_006501764.1 probable ATP-dependent RNA helicase DDX20 isoform X1
XM_006501701.4 XP_006501764.1 probable ATP-dependent RNA helicase DDX20 isoform X1

Pathways from BioSystems


Homologies


Bos taurus (cattle) DDX20 NP_001095588.1
Pan troglodytes (chimpanzee) DDX20 XP_003308378.1
Danio rerio (zebrafish) ddx20 NP_001116795.1
Rattus norvegicus (Norway rat) Ddx20 NP_001178640.1
Canis lupus familiaris (dog) DDX20 XP_003639658.1
Mus musculus (house mouse) Ddx20 NP_059093.3
Gallus gallus (chicken) DDX20 XP_417996.1
Homo sapiens (human) DDX20 NP_009135.4
Xenopus tropicalis (tropical clawed frog) ddx20 XP_002932842.1

Gene Ontology


Bibliography

Related articles in PubMed

DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.
Mouillet JF, Yan X, Ou Q, Jin L, Muglia LJ, Crawford PA, Sadovsky Y
Endocrinology149(5)2168-75(2008 May)

Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis.
Lee K, Pisarska MD, Ko JJ, Kang Y, Yoon S, Ryou SM, Cha KY, Bae J
Biochemical and biophysical research communications336(3)876-81(2005 Oct)

Temporal and spatial transcriptional programs in murine kidney development.
Challen G, Gardiner B, Caruana G, Kostoulias X, Martinez G, Crowe M, Taylor DF, Bertram J, Little M, Grimmond SM
Physiological genomics23(2)159-71(2005 Oct)

The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.
Lee MB, Lebedeva LA, Suzawa M, Wadekar SA, Desclozeaux M, Ingraham HA
Molecular and cellular biology25(5)1879-90(2005 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Diverse functions for murine DP103, with an obligatory role during early embryonic development and also in modulation of steroidogenesis.
Title: DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.

DP103 directly interacts with sumoylated SF-1 and is coupled to transcriptional repression by protein sumoylation.
Title: The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.

Ddx20 repressed Egr2-mediated transcriptional activation with significant promoter specificity
Title: The Ddx20/DP103 dead box protein represses transcriptional activation by Egr2/Krox-20.

The following Ddx20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu10754 NM_017397.3
Latest version!		 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu10754 XM_006501701.4
Latest version!		 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OMu10754 XM_006501701.2 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu10754 XM_006501701.3 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu10754
Clone ID Related Accession (Same CDS sequence) NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version NM_017397.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2478bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-26
Organism Mus musculus(house mouse)
Product probable ATP-dependent RNA helicase DDX20
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125471.1. On Jun 3, 2007 this sequence version replaced NM_017397.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125471.1, AF220454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_059093.3
CDS145..2622
Translation

Target ORF information:

RefSeq Version NM_017397.3
Organism Mus musculus(house mouse)
Definition Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_017397.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu10754
Clone ID Related Accession (Same CDS sequence) NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version XM_006501701.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2478bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product probable ATP-dependent RNA helicase DDX20 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006501764.1
CDS518..2995
Translation

Target ORF information:

RefSeq Version XM_006501701.3
Organism Mus musculus(house mouse)
Definition Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006501701.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu10754
Clone ID Related Accession (Same CDS sequence) NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version XM_006501701.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2478bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-02-08
Organism Mus musculus(House mouse)
Product probable ATP-dependent RNA helicase DDX20 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006501764.1
Translation

Target ORF information:

RefSeq Version XM_006501701.2
Organism Mus musculus(House mouse)
Definition Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006501701.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu10754
Clone ID Related Accession (Same CDS sequence) NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version XM_006501701.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2478bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product probable ATP-dependent RNA helicase DDX20 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501701.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006501764.1
CDS188..2665
Translation

Target ORF information:

RefSeq Version XM_006501701.4
Organism Mus musculus(house mouse)
Definition Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006501701.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.
Endocrinology149(5)2168-75(2008 May)
Mouillet JF,Yan X,Ou Q,Jin L,Muglia LJ,Crawford PA,Sadovsky Y


book

Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis.
Biochemical and biophysical research communications336(3)876-81(2005 Oct)
Lee K,Pisarska MD,Ko JJ,Kang Y,Yoon S,Ryou SM,Cha KY,Bae J


book

Temporal and spatial transcriptional programs in murine kidney development.
Physiological genomics23(2)159-71(2005 Oct)
Challen G,Gardiner B,Caruana G,Kostoulias X,Martinez G,Crowe M,Taylor DF,Bertram J,Little M,Grimmond SM


book

The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.
Molecular and cellular biology25(5)1879-90(2005 Mar)
Lee MB,Lebedeva LA,Suzawa M,Wadekar SA,Desclozeaux M,Ingraham HA


book

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE