P3h1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	P3h1
Entrez Gene ID	56401
Full Name	prolyl 3-hydroxylase 1
Synonyms	2410024C15Rik,Gros1,Lepre1
General protein information	Preferred Names prolyl 3-hydroxylase 1 Names prolyl 3-hydroxylase 1 growth suppressor 1 leprecan 1 leucine- and proline-enriched proteoglycan 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286148.1	NP_001273077.1	prolyl 3-hydroxylase 1 isoform 4 precursor
NM_019783.2	NP_062757.2	prolyl 3-hydroxylase 1 isoform 2 precursor
NM_001042411.1	NP_001035874.1	prolyl 3-hydroxylase 1 isoform 3
NM_019782.3	NP_062756.2	prolyl 3-hydroxylase 1 isoform 1 precursor

Pathways from BioSystems

Homologies

	LEPRE1	XP_001088904.1
Mus musculus (house mouse)	Lepre1	NP_062757.2
Homo sapiens (human)	LEPRE1	NP_071751.3
Rattus norvegicus (Norway rat)	Lepre1	NP_446119.1
Gallus gallus (chicken)	LEPRE1	NP_001001529.1
Danio rerio (zebrafish)	lepre1	XP_002663865.1
Xenopus tropicalis (tropical clawed frog)	lepre1	NP_001090700.1
Pan troglodytes (chimpanzee)	LEPRE1	XP_003308086.1
Canis lupus familiaris (dog)	LEPRE1	XP_848570.2
Bos taurus (cattle)	LEPRE1	NP_001096761.1

Gene Ontology
Bibliography

Related articles in PubMed

Bone matrix hypermineralization in prolyl-3 hydroxylase 1 deficient mice.
Fratzl-Zelman N, B?chinger HP, Vranka JA, Roschger P, Klaushofer K, Rauch F
Bone8515-22(2016 Apr)

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Street VA, Kujawa SG, Manichaikul A, Broman KW, Kallman JC, Shilling DJ, Iwata AJ, Robinson LC, Robbins CA, Li J, Liberman MC, Tempel BL
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)

Differential effects of collagen prolyl 3-hydroxylation on skeletal tissues.
Homan EP, Lietman C, Grafe I, Lennington J, Morello R, Napierala D, Jiang MM, Munivez EM, Dawson B, Bertin TK, Chen Y, Lua R, Lichtarge O, Hicks J, Weis MA, Eyre D, Lee BH
PLoS genetics10(1)e1004121(2014 Jan)

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
Ishikawa Y, B?chinger HP
The Journal of biological chemistry288(44)31437-46(2013 Nov)

Posttranslational modifications in type I collagen from different tissues extracted from wild type and prolyl 3-hydroxylase 1 null mice.
Pokidysheva E, Zientek KD, Ishikawa Y, Mizuno K, Vranka JA, Montgomery NT, Keene DR, Kawaguchi T, Okuyama K, B?chinger HP
The Journal of biological chemistry288(34)24742-52(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

P3H1 deficiency leads to decreased deposition of extracellular matrix by osteoblasts and increased incorporation of mineral into the matrix.
Title: Bone matrix hypermineralization in prolyl-3 hydroxylase 1 deficient mice.

we generated knock-in mice carrying a single amino acid substitution in the catalytic site of P3h1 (Lepre1(H662A) ). This substitution abolished P3h1 activity but retained ability to form a complex with Crtap and thus the collagen chaperone
Title: Differential effects of collagen prolyl 3-hydroxylation on skeletal tissues.

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
Title: An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.

The absence of 3-hydroxyproline and/or the increased glycosylation of hydroxylysine in type I collagen disturbs the lateral growth of the fibrils.
Title: Posttranslational modifications in type I collagen from different tissues extracted from wild type and prolyl 3-hydroxylase 1 null mice.

The LEPRE1 knockout mouse is a valuable model system to investigate the mechanism of hearing loss in recessive osteogenesis imperfecta.
Title: Prolyl 3-hydroxylase-1 null mice exhibit hearing impairment and abnormal morphology of the middle ear bone joints.

The following P3h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00877	NM_001042411.1 Latest version!	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00
OMu11450	NM_019782.3 Latest version!	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$440.30 $629.00
OMu11451	NM_019783.2 Latest version!	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
OMu17163	NM_001286148.1 Latest version!	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3h1 ( NM_001042411.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001035874.1 Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu00877
Clone ID Related Accession (Same CDS sequence)	NM_001042411.1
Accession Version	NM_001042411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY240019.1, AK159505.1, AK010578.1, AK030436.1 and CO040878.1. Transcript Variant: This variant (3) differs in the 5' and 3' UTRs, initiates translation at a downstream start codon and uses alternate splice sites in the 3' coding region, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159505.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGATGC GGCAGAACCT CGACTATTAC CAAACCATGT CTGGGGTGAA GGAGGCAGAC 
TTCAGGGATC TCGAGGCCAA GCCCCATATG CATGAGTTTC GGCTGGGGGT ACGACTCTAC 
TCAGAGGAGA AGCCACAGGA AGCTGTGCCC CACCTGGAGG CGGCACTGCA AGAGTACTTT 
GTGGCCGATG AGGAATGCCG TGCCCTCTGC GAAGGGCCCT ATGACTACGA CGGCTACAAC 
TACCTAGACT ACAGCGCTGA CCTCTTCCAG GCCATCACAG ATCATTACGT CCAGGTCCTC 
AACTGTAAGC AGAACTGTGT CACGGAGCTG GCTTCCCACC CAAGTAGGGA AAAGCCCTTT 
GAAGACTTCC TCCCTTCACA CTATAATTAC CTACAGTTTG CCTACTACAA CATTGGGAAC 
TATACACAAG CTATTGAATG TGCCAAGACC TACCTCCTCT TCTTTCCCAA TGATGAGGTG 
ATGCACCAGA ATCTGGCTTA TTACACAGCC ATGCTTGGAG AAGAAGAGGC CAGCTCCATC 
AGCCCCAGGG AGAATGCCGA GGAATACCGA CGTCGAAGCC TGTTGGAGAA AGAACTGCTT 
TTCTTCGCTT ATGACATTTT TGGAATTCCC TTTGTGGATC CGGACTCATG GACTCCAGAA 
GAGGTGATTC CCAAGAGACT GCAGGAGAAA CAGAAGTCGG AACGCGAAAC CGCAGTCCGC 
ATCTCCCAGG AGATTGGCAA CCTTATGAAG GAGATCGAGA CCCTCGTGGA GGAGAAGACC 
AAGGAGTCCT TGGATGTGAG CCGACTGACG CGGGAAGGTG GTCCCCTGCT GTATGAAGGC 
ATCAGTCTCA CCATGAATTC CAAAGTCTTG AATGGCTCCC AGCGGGTGGT GATGGATGGT 
GTGATCTCTG ATGATGAGTG CCAGGAGCTG CAGAGACTGA CCAATGCGGC AGCAACTTCG 
GGAGATGGCT ACCGAGGTCA GACCTCCCCA CACACCCCAA ATGAAAAGTT CTATGGTGTT 
ACTGTCCTCA AAGCTCTCAA GCTCGGGCAG GAAGGAAAAG TTCCTCTGCA GAGTGCCCGC 
ATGTACTACA ACGTGACAGA GAAGGTGCGG CGCGTCATGG AGTCCTACTT CCGCCTGGAC 
ACGCCCCTCT ATTTCTCTTA TTCCCACCTC GTGTGCCGCA CTGCAATAGA AGAGTCACAG 
GCTGAGAGGA AGGACAGTAG CCACCCCGTC CACGTGGATA ACTGCATCCT GAATGCCGAA 
GCCCTCATGT GTATCAAGGA GCCCCCAGCC TACACGTTCC GGGACTACAG CGCCATCCTT 
TACCTCAATG GCGACTTCGA TGGAGGAAAC TTTTACTTCA CAGAACTAGA TGCCAAGACT 
GTGACGGCAG AGGTGCAGCC CCAGTGTGGA AGGGCTGTGG GATTCTCTTC TGGCACTGAG 
AACCCACATG GAGTGAAGGC TGTCACCAGG GGGCAGCGCT GCGCCATCGC CCTGTGGTTC 
ACGCTGGATC CTCGGCACAG TGAGAGAGAC AGGGTGCAGG CAGATGACCT GGTGAAGATG 
CTGTTCAGCC CAGAAGAGGT GGACCTCCCC CAGGAACAGC CCCTGCCTGA CCAGCAGGGC 
TCGCCAGAGC CTGGAGAAGA GTCTCTGTCA GACAGAGGGT CGCTGCACAA GGATGAGCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035874.1
CDS	214..1896
Translation	MEMRQNLDYYQTMSGVKEADFRDLEAKPHMHEFRLGVRLYSEEKPQEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYVQVLNCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFFPNDEVMHQNLAYYTAMLGEEEASSISPRENAEEYRRRSLLEKELLFFAYDIFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKVLNGSQRVVMDGVISDDECQELQRLTNAAATSGDGYRGQTSPHTPNEKFYGVTVLKALKLGQEGKVPLQSARMYYNVTEKVRRVMESYFRLDTPLYFSYSHLVCRTAIEESQAERKDSSHPVHVDNCILNAEALMCIKEPPAYTFRDYSAILYLNGDFDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSPEEVDLPQEQPLPDQQGSPEPGEESLSDRGSLHKDEL

Target ORF information:

RefSeq Version	NM_001042411.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042411.1

ORF Insert Sequence:

ATGGAGATGC GGCAGAACCT CGACTATTAC CAAACCATGT CTGGGGTGAA GGAGGCAGAC 
TTCAGGGATC TCGAGGCCAA GCCCCATATG CATGAGTTTC GGCTGGGGGT ACGACTCTAC 
TCAGAGGAGA AGCCACAGGA AGCTGTGCCC CACCTGGAGG CGGCACTGCA AGAGTACTTT 
GTGGCCGATG AGGAATGCCG TGCCCTCTGC GAAGGGCCCT ATGACTACGA CGGCTACAAC 
TACCTAGACT ACAGCGCTGA CCTCTTCCAG GCCATCACAG ATCATTACGT CCAGGTCCTC 
AACTGTAAGC AGAACTGTGT CACGGAGCTG GCTTCCCACC CAAGTAGGGA AAAGCCCTTT 
GAAGACTTCC TCCCTTCACA CTATAATTAC CTACAGTTTG CCTACTACAA CATTGGGAAC 
TATACACAAG CTATTGAATG TGCCAAGACC TACCTCCTCT TCTTTCCCAA TGATGAGGTG 
ATGCACCAGA ATCTGGCTTA TTACACAGCC ATGCTTGGAG AAGAAGAGGC CAGCTCCATC 
AGCCCCAGGG AGAATGCCGA GGAATACCGA CGTCGAAGCC TGTTGGAGAA AGAACTGCTT 
TTCTTCGCTT ATGACATTTT TGGAATTCCC TTTGTGGATC CGGACTCATG GACTCCAGAA 
GAGGTGATTC CCAAGAGACT GCAGGAGAAA CAGAAGTCGG AACGCGAAAC CGCAGTCCGC 
ATCTCCCAGG AGATTGGCAA CCTTATGAAG GAGATCGAGA CCCTCGTGGA GGAGAAGACC 
AAGGAGTCCT TGGATGTGAG CCGACTGACG CGGGAAGGTG GTCCCCTGCT GTATGAAGGC 
ATCAGTCTCA CCATGAATTC CAAAGTCTTG AATGGCTCCC AGCGGGTGGT GATGGATGGT 
GTGATCTCTG ATGATGAGTG CCAGGAGCTG CAGAGACTGA CCAATGCGGC AGCAACTTCG 
GGAGATGGCT ACCGAGGTCA GACCTCCCCA CACACCCCAA ATGAAAAGTT CTATGGTGTT 
ACTGTCCTCA AAGCTCTCAA GCTCGGGCAG GAAGGAAAAG TTCCTCTGCA GAGTGCCCGC 
ATGTACTACA ACGTGACAGA GAAGGTGCGG CGCGTCATGG AGTCCTACTT CCGCCTGGAC 
ACGCCCCTCT ATTTCTCTTA TTCCCACCTC GTGTGCCGCA CTGCAATAGA AGAGTCACAG 
GCTGAGAGGA AGGACAGTAG CCACCCCGTC CACGTGGATA ACTGCATCCT GAATGCCGAA 
GCCCTCATGT GTATCAAGGA GCCCCCAGCC TACACGTTCC GGGACTACAG CGCCATCCTT 
TACCTCAATG GCGACTTCGA TGGAGGAAAC TTTTACTTCA CAGAACTAGA TGCCAAGACT 
GTGACGGCAG AGGTGCAGCC CCAGTGTGGA AGGGCTGTGG GATTCTCTTC TGGCACTGAG 
AACCCACATG GAGTGAAGGC TGTCACCAGG GGGCAGCGCT GCGCCATCGC CCTGTGGTTC 
ACGCTGGATC CTCGGCACAG TGAGAGAGAC AGGGTGCAGG CAGATGACCT GGTGAAGATG 
CTGTTCAGCC CAGAAGAGGT GGACCTCCCC CAGGAACAGC CCCTGCCTGA CCAGCAGGGC 
TCGCCAGAGC CTGGAGAAGA GTCTCTGTCA GACAGAGGGT CGCTGCACAA GGATGAGCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h1 ( NM_019782.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062756.2 Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11450
Clone ID Related Accession (Same CDS sequence)	NM_019782.3
Accession Version	NM_019782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK010578.1, CX563255.1, AK030436.1 and CO040878.1. On Oct 31, 2013 this sequence version replaced NM_019782.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGA GCGAAAGGAG GCTGCTGGCA GCGATGCTAG CGGTCGCCGC CGCCGCCGCC 
TTACGGGTTG CGGCGGAGTC TGAGCCGGGA TGGGACGTGG CAGCCCCTGA CCTGCTTTAC 
GCAGAGGGGA CCGCGGCCTA CTCGCGCGGG GACTGGCCCG GGGTGGTCCT GAACATGGAG 
CGGGCGCTGC GCTCGCGGGC GGCCCTGCGT GCCCTCCGCC TGCGCTGCCG CACACGCTGT 
GCCACCGAAC TGCCGTGGGC ACCGGACCTG GATCTCGGTC CGGACCCCAG CCTGAGCCAG 
GACCCGGGCG CCGCCGCCCT GCACGACCTG CGCTTCTTCG GAGCCGTGCT GCGCCGTGCC 
GCCTGCCTAC GCCGCTGCCT CGGGCCGCCC TCTGCCCACT TGCTGAGTGA AGAACTGGAC 
CTGGAGTTCA ACAAGCGGAG CCCGTACAAC TACCTGCAGG TCGCCTATTT CAAGATAAAC 
AAGCTGGAGA AAGCTGTGGC TGCGGCACAC ACCTTCTTTG TGGGCAATCC TGAGCACATG 
GAGATGCGGC AGAACCTCGA CTATTACCAA ACCATGTCTG GGGTGAAGGA GGCAGACTTC 
AGGGATCTCG AGGCCAAGCC CCATATGCAT GAGTTTCGGC TGGGGGTACG ACTCTACTCA 
GAGGAGAAGC CACAGGAAGC TGTGCCCCAC CTGGAGGCGG CACTGCAAGA GTACTTTGTG 
GCCGATGAGG AATGCCGTGC CCTCTGCGAA GGGCCCTATG ACTACGACGG CTACAACTAC 
CTAGACTACA GCGCTGACCT CTTCCAGGCC ATCACAGATC ATTACGTCCA GGTCCTCAAC 
TGTAAGCAGA ACTGTGTCAC GGAGCTGGCT TCCCACCCAA GTAGGGAAAA GCCCTTTGAA 
GACTTCCTCC CTTCACACTA TAATTACCTA CAGTTTGCCT ACTACAACAT TGGGAACTAT 
ACACAAGCTA TTGAATGTGC CAAGACCTAC CTCCTCTTCT TTCCCAATGA TGAGGTGATG 
CACCAGAATC TGGCTTATTA CACAGCCATG CTTGGAGAAG AAGAGGCCAG CTCCATCAGC 
CCCAGGGAGA ATGCCGAGGA ATACCGACGT CGAAGCCTGT TGGAGAAAGA ACTGCTTTTC 
TTCGCTTATG ACATTTTTGG AATTCCCTTT GTGGATCCGG ACTCATGGAC TCCAGAAGAG 
GTGATTCCCA AGAGACTGCA GGAGAAACAG AAGTCGGAAC GCGAAACCGC AGTCCGCATC 
TCCCAGGAGA TTGGCAACCT TATGAAGGAG ATCGAGACCC TCGTGGAGGA GAAGACCAAG 
GAGTCCTTGG ATGTGAGCCG ACTGACGCGG GAAGGTGGTC CCCTGCTGTA TGAAGGCATC 
AGTCTCACCA TGAATTCCAA AGTCTTGAAT GGCTCCCAGC GGGTGGTGAT GGATGGTGTG 
ATCTCTGATG ATGAGTGCCA GGAGCTGCAG AGACTGACCA ATGCGGCAGC AACTTCGGGA 
GATGGCTACC GAGGTCAGAC CTCCCCACAC ACCCCAAATG AAAAGTTCTA TGGTGTTACT 
GTCCTCAAAG CTCTCAAGCT CGGGCAGGAA GGAAAAGTTC CTCTGCAGAG TGCCCGCATG 
TACTACAACG TGACAGAGAA GGTGCGGCGC GTCATGGAGT CCTACTTCCG CCTGGACACG 
CCCCTCTATT TCTCTTATTC CCACCTCGTG TGCCGCACTG CAATAGAAGA GTCACAGGCT 
GAGAGGAAGG ACAGTAGCCA CCCCGTCCAC GTGGATAACT GCATCCTGAA TGCCGAAGCC 
CTCATGTGTA TCAAGGAGCC CCCAGCCTAC ACGTTCCGGG ACTACAGCGC CATCCTTTAC 
CTCAATGGCG ACTTCGATGG AGGAAACTTT TACTTCACAG AACTAGATGC CAAGACTGTG 
ACGGCAGAGG TGCAGCCCCA GTGTGGAAGG GCTGTGGGAT TCTCTTCTGG CACTGAGAAC 
CCACATGGAG TGAAGGCTGT CACCAGGGGG CAGCGCTGCG CCATCGCCCT GTGGTTCACG 
CTGGATCCTC GGCACAGTGA GAGAGACAGG GTGCAGGCAG ATGACCTGGT GAAGATGCTG 
TTCAGCCCAG AAGAGGTGGA CCTCCCCCAG GAACAGCCCC TGCCTGACCA GCAGGGCTCG 
CCAGAGCCTG GAGAAGACCT TCTGCATGGT GCTACTGTTC TTGGAGTGGG CATAGCAGGA 
CACACTCTTC TCTGGGCTTG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062756.2
CDS	43..2289
Translation	MAVSERRLLAAMLAVAAAAALRVAAESEPGWDVAAPDLLYAEGTAAYSRGDWPGVVLNMERALRSRAALRALRLRCRTRCATELPWAPDLDLGPDPSLSQDPGAAALHDLRFFGAVLRRAACLRRCLGPPSAHLLSEELDLEFNKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMRQNLDYYQTMSGVKEADFRDLEAKPHMHEFRLGVRLYSEEKPQEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYVQVLNCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFFPNDEVMHQNLAYYTAMLGEEEASSISPRENAEEYRRRSLLEKELLFFAYDIFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKVLNGSQRVVMDGVISDDECQELQRLTNAAATSGDGYRGQTSPHTPNEKFYGVTVLKALKLGQEGKVPLQSARMYYNVTEKVRRVMESYFRLDTPLYFSYSHLVCRTAIEESQAERKDSSHPVHVDNCILNAEALMCIKEPPAYTFRDYSAILYLNGDFDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSPEEVDLPQEQPLPDQQGSPEPGEDLLHGATVLGVGIAGHTLLWAWL

Target ORF information:

RefSeq Version	NM_019782.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019782.3

ORF Insert Sequence:

ATGGCGGTGA GCGAAAGGAG GCTGCTGGCA GCGATGCTAG CGGTCGCCGC CGCCGCCGCC 
TTACGGGTTG CGGCGGAGTC TGAGCCGGGA TGGGACGTGG CAGCCCCTGA CCTGCTTTAC 
GCAGAGGGGA CCGCGGCCTA CTCGCGCGGG GACTGGCCCG GGGTGGTCCT GAACATGGAG 
CGGGCGCTGC GCTCGCGGGC GGCCCTGCGT GCCCTCCGCC TGCGCTGCCG CACACGCTGT 
GCCACCGAAC TGCCGTGGGC ACCGGACCTG GATCTCGGTC CGGACCCCAG CCTGAGCCAG 
GACCCGGGCG CCGCCGCCCT GCACGACCTG CGCTTCTTCG GAGCCGTGCT GCGCCGTGCC 
GCCTGCCTAC GCCGCTGCCT CGGGCCGCCC TCTGCCCACT TGCTGAGTGA AGAACTGGAC 
CTGGAGTTCA ACAAGCGGAG CCCGTACAAC TACCTGCAGG TCGCCTATTT CAAGATAAAC 
AAGCTGGAGA AAGCTGTGGC TGCGGCACAC ACCTTCTTTG TGGGCAATCC TGAGCACATG 
GAGATGCGGC AGAACCTCGA CTATTACCAA ACCATGTCTG GGGTGAAGGA GGCAGACTTC 
AGGGATCTCG AGGCCAAGCC CCATATGCAT GAGTTTCGGC TGGGGGTACG ACTCTACTCA 
GAGGAGAAGC CACAGGAAGC TGTGCCCCAC CTGGAGGCGG CACTGCAAGA GTACTTTGTG 
GCCGATGAGG AATGCCGTGC CCTCTGCGAA GGGCCCTATG ACTACGACGG CTACAACTAC 
CTAGACTACA GCGCTGACCT CTTCCAGGCC ATCACAGATC ATTACGTCCA GGTCCTCAAC 
TGTAAGCAGA ACTGTGTCAC GGAGCTGGCT TCCCACCCAA GTAGGGAAAA GCCCTTTGAA 
GACTTCCTCC CTTCACACTA TAATTACCTA CAGTTTGCCT ACTACAACAT TGGGAACTAT 
ACACAAGCTA TTGAATGTGC CAAGACCTAC CTCCTCTTCT TTCCCAATGA TGAGGTGATG 
CACCAGAATC TGGCTTATTA CACAGCCATG CTTGGAGAAG AAGAGGCCAG CTCCATCAGC 
CCCAGGGAGA ATGCCGAGGA ATACCGACGT CGAAGCCTGT TGGAGAAAGA ACTGCTTTTC 
TTCGCTTATG ACATTTTTGG AATTCCCTTT GTGGATCCGG ACTCATGGAC TCCAGAAGAG 
GTGATTCCCA AGAGACTGCA GGAGAAACAG AAGTCGGAAC GCGAAACCGC AGTCCGCATC 
TCCCAGGAGA TTGGCAACCT TATGAAGGAG ATCGAGACCC TCGTGGAGGA GAAGACCAAG 
GAGTCCTTGG ATGTGAGCCG ACTGACGCGG GAAGGTGGTC CCCTGCTGTA TGAAGGCATC 
AGTCTCACCA TGAATTCCAA AGTCTTGAAT GGCTCCCAGC GGGTGGTGAT GGATGGTGTG 
ATCTCTGATG ATGAGTGCCA GGAGCTGCAG AGACTGACCA ATGCGGCAGC AACTTCGGGA 
GATGGCTACC GAGGTCAGAC CTCCCCACAC ACCCCAAATG AAAAGTTCTA TGGTGTTACT 
GTCCTCAAAG CTCTCAAGCT CGGGCAGGAA GGAAAAGTTC CTCTGCAGAG TGCCCGCATG 
TACTACAACG TGACAGAGAA GGTGCGGCGC GTCATGGAGT CCTACTTCCG CCTGGACACG 
CCCCTCTATT TCTCTTATTC CCACCTCGTG TGCCGCACTG CAATAGAAGA GTCACAGGCT 
GAGAGGAAGG ACAGTAGCCA CCCCGTCCAC GTGGATAACT GCATCCTGAA TGCCGAAGCC 
CTCATGTGTA TCAAGGAGCC CCCAGCCTAC ACGTTCCGGG ACTACAGCGC CATCCTTTAC 
CTCAATGGCG ACTTCGATGG AGGAAACTTT TACTTCACAG AACTAGATGC CAAGACTGTG 
ACGGCAGAGG TGCAGCCCCA GTGTGGAAGG GCTGTGGGAT TCTCTTCTGG CACTGAGAAC 
CCACATGGAG TGAAGGCTGT CACCAGGGGG CAGCGCTGCG CCATCGCCCT GTGGTTCACG 
CTGGATCCTC GGCACAGTGA GAGAGACAGG GTGCAGGCAG ATGACCTGGT GAAGATGCTG 
TTCAGCCCAG AAGAGGTGGA CCTCCCCCAG GAACAGCCCC TGCCTGACCA GCAGGGCTCG 
CCAGAGCCTG GAGAAGACCT TCTGCATGGT GCTACTGTTC TTGGAGTGGG CATAGCAGGA 
CACACTCTTC TCTGGGCTTG GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h1 ( NM_019783.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062757.2 Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 2, mRNA.

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CloneID	OMu11451
Clone ID Related Accession (Same CDS sequence)	NM_019783.2
Accession Version	NM_019783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK010578.1, AK030436.1 and CO040878.1. On Jun 16, 2006 this sequence version replaced NM_019783.1. Transcript Variant: This variant (2) uses alternate splice sites in the 3' coding region and differs in the 3' UTR, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030436.1, SRR1660819.181903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGA GCGAAAGGAG GCTGCTGGCA GCGATGCTAG CGGTCGCCGC CGCCGCCGCC 
TTACGGGTTG CGGCGGAGTC TGAGCCGGGA TGGGACGTGG CAGCCCCTGA CCTGCTTTAC 
GCAGAGGGGA CCGCGGCCTA CTCGCGCGGG GACTGGCCCG GGGTGGTCCT GAACATGGAG 
CGGGCGCTGC GCTCGCGGGC GGCCCTGCGT GCCCTCCGCC TGCGCTGCCG CACACGCTGT 
GCCACCGAAC TGCCGTGGGC ACCGGACCTG GATCTCGGTC CGGACCCCAG CCTGAGCCAG 
GACCCGGGCG CCGCCGCCCT GCACGACCTG CGCTTCTTCG GAGCCGTGCT GCGCCGTGCC 
GCCTGCCTAC GCCGCTGCCT CGGGCCGCCC TCTGCCCACT TGCTGAGTGA AGAACTGGAC 
CTGGAGTTCA ACAAGCGGAG CCCGTACAAC TACCTGCAGG TCGCCTATTT CAAGATAAAC 
AAGCTGGAGA AAGCTGTGGC TGCGGCACAC ACCTTCTTTG TGGGCAATCC TGAGCACATG 
GAGATGCGGC AGAACCTCGA CTATTACCAA ACCATGTCTG GGGTGAAGGA GGCAGACTTC 
AGGGATCTCG AGGCCAAGCC CCATATGCAT GAGTTTCGGC TGGGGGTACG ACTCTACTCA 
GAGGAGAAGC CACAGGAAGC TGTGCCCCAC CTGGAGGCGG CACTGCAAGA GTACTTTGTG 
GCCGATGAGG AATGCCGTGC CCTCTGCGAA GGGCCCTATG ACTACGACGG CTACAACTAC 
CTAGACTACA GCGCTGACCT CTTCCAGGCC ATCACAGATC ATTACGTCCA GGTCCTCAAC 
TGTAAGCAGA ACTGTGTCAC GGAGCTGGCT TCCCACCCAA GTAGGGAAAA GCCCTTTGAA 
GACTTCCTCC CTTCACACTA TAATTACCTA CAGTTTGCCT ACTACAACAT TGGGAACTAT 
ACACAAGCTA TTGAATGTGC CAAGACCTAC CTCCTCTTCT TTCCCAATGA TGAGGTGATG 
CACCAGAATC TGGCTTATTA CACAGCCATG CTTGGAGAAG AAGAGGCCAG CTCCATCAGC 
CCCAGGGAGA ATGCCGAGGA ATACCGACGT CGAAGCCTGT TGGAGAAAGA ACTGCTTTTC 
TTCGCTTATG ACATTTTTGG AATTCCCTTT GTGGATCCGG ACTCATGGAC TCCAGAAGAG 
GTGATTCCCA AGAGACTGCA GGAGAAACAG AAGTCGGAAC GCGAAACCGC AGTCCGCATC 
TCCCAGGAGA TTGGCAACCT TATGAAGGAG ATCGAGACCC TCGTGGAGGA GAAGACCAAG 
GAGTCCTTGG ATGTGAGCCG ACTGACGCGG GAAGGTGGTC CCCTGCTGTA TGAAGGCATC 
AGTCTCACCA TGAATTCCAA AGTCTTGAAT GGCTCCCAGC GGGTGGTGAT GGATGGTGTG 
ATCTCTGATG ATGAGTGCCA GGAGCTGCAG AGACTGACCA ATGCGGCAGC AACTTCGGGA 
GATGGCTACC GAGGTCAGAC CTCCCCACAC ACCCCAAATG AAAAGTTCTA TGGTGTTACT 
GTCCTCAAAG CTCTCAAGCT CGGGCAGGAA GGAAAAGTTC CTCTGCAGAG TGCCCGCATG 
TACTACAACG TGACAGAGAA GGTGCGGCGC GTCATGGAGT CCTACTTCCG CCTGGACACG 
CCCCTCTATT TCTCTTATTC CCACCTCGTG TGCCGCACTG CAATAGAAGA GTCACAGGCT 
GAGAGGAAGG ACAGTAGCCA CCCCGTCCAC GTGGATAACT GCATCCTGAA TGCCGAAGCC 
CTCATGTGTA TCAAGGAGCC CCCAGCCTAC ACGTTCCGGG ACTACAGCGC CATCCTTTAC 
CTCAATGGCG ACTTCGATGG AGGAAACTTT TACTTCACAG AACTAGATGC CAAGACTGTG 
ACGGCAGAGG TGCAGCCCCA GTGTGGAAGG GCTGTGGGAT TCTCTTCTGG CACTGAGAAC 
CCACATGGAG TGAAGGCTGT CACCAGGGGG CAGCGCTGCG CCATCGCCCT GTGGTTCACG 
CTGGATCCTC GGCACAGTGA GAGAGACAGG GTGCAGGCAG ATGACCTGGT GAAGATGCTG 
TTCAGCCCAG AAGAGGTGGA CCTCCCCCAG GAACAGCCCC TGCCTGACCA GCAGGGCTCG 
CCAGAGCCTG GAGAAGAGTC TCTGTCAGAC AGAGGGTCGC TGCACAAGGA TGAGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062757.2
CDS	43..2262
Translation	MAVSERRLLAAMLAVAAAAALRVAAESEPGWDVAAPDLLYAEGTAAYSRGDWPGVVLNMERALRSRAALRALRLRCRTRCATELPWAPDLDLGPDPSLSQDPGAAALHDLRFFGAVLRRAACLRRCLGPPSAHLLSEELDLEFNKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMRQNLDYYQTMSGVKEADFRDLEAKPHMHEFRLGVRLYSEEKPQEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYVQVLNCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFFPNDEVMHQNLAYYTAMLGEEEASSISPRENAEEYRRRSLLEKELLFFAYDIFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKVLNGSQRVVMDGVISDDECQELQRLTNAAATSGDGYRGQTSPHTPNEKFYGVTVLKALKLGQEGKVPLQSARMYYNVTEKVRRVMESYFRLDTPLYFSYSHLVCRTAIEESQAERKDSSHPVHVDNCILNAEALMCIKEPPAYTFRDYSAILYLNGDFDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSPEEVDLPQEQPLPDQQGSPEPGEESLSDRGSLHKDEL

Target ORF information:

RefSeq Version	NM_019783.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019783.2

ORF Insert Sequence:

ATGGCGGTGA GCGAAAGGAG GCTGCTGGCA GCGATGCTAG CGGTCGCCGC CGCCGCCGCC 
TTACGGGTTG CGGCGGAGTC TGAGCCGGGA TGGGACGTGG CAGCCCCTGA CCTGCTTTAC 
GCAGAGGGGA CCGCGGCCTA CTCGCGCGGG GACTGGCCCG GGGTGGTCCT GAACATGGAG 
CGGGCGCTGC GCTCGCGGGC GGCCCTGCGT GCCCTCCGCC TGCGCTGCCG CACACGCTGT 
GCCACCGAAC TGCCGTGGGC ACCGGACCTG GATCTCGGTC CGGACCCCAG CCTGAGCCAG 
GACCCGGGCG CCGCCGCCCT GCACGACCTG CGCTTCTTCG GAGCCGTGCT GCGCCGTGCC 
GCCTGCCTAC GCCGCTGCCT CGGGCCGCCC TCTGCCCACT TGCTGAGTGA AGAACTGGAC 
CTGGAGTTCA ACAAGCGGAG CCCGTACAAC TACCTGCAGG TCGCCTATTT CAAGATAAAC 
AAGCTGGAGA AAGCTGTGGC TGCGGCACAC ACCTTCTTTG TGGGCAATCC TGAGCACATG 
GAGATGCGGC AGAACCTCGA CTATTACCAA ACCATGTCTG GGGTGAAGGA GGCAGACTTC 
AGGGATCTCG AGGCCAAGCC CCATATGCAT GAGTTTCGGC TGGGGGTACG ACTCTACTCA 
GAGGAGAAGC CACAGGAAGC TGTGCCCCAC CTGGAGGCGG CACTGCAAGA GTACTTTGTG 
GCCGATGAGG AATGCCGTGC CCTCTGCGAA GGGCCCTATG ACTACGACGG CTACAACTAC 
CTAGACTACA GCGCTGACCT CTTCCAGGCC ATCACAGATC ATTACGTCCA GGTCCTCAAC 
TGTAAGCAGA ACTGTGTCAC GGAGCTGGCT TCCCACCCAA GTAGGGAAAA GCCCTTTGAA 
GACTTCCTCC CTTCACACTA TAATTACCTA CAGTTTGCCT ACTACAACAT TGGGAACTAT 
ACACAAGCTA TTGAATGTGC CAAGACCTAC CTCCTCTTCT TTCCCAATGA TGAGGTGATG 
CACCAGAATC TGGCTTATTA CACAGCCATG CTTGGAGAAG AAGAGGCCAG CTCCATCAGC 
CCCAGGGAGA ATGCCGAGGA ATACCGACGT CGAAGCCTGT TGGAGAAAGA ACTGCTTTTC 
TTCGCTTATG ACATTTTTGG AATTCCCTTT GTGGATCCGG ACTCATGGAC TCCAGAAGAG 
GTGATTCCCA AGAGACTGCA GGAGAAACAG AAGTCGGAAC GCGAAACCGC AGTCCGCATC 
TCCCAGGAGA TTGGCAACCT TATGAAGGAG ATCGAGACCC TCGTGGAGGA GAAGACCAAG 
GAGTCCTTGG ATGTGAGCCG ACTGACGCGG GAAGGTGGTC CCCTGCTGTA TGAAGGCATC 
AGTCTCACCA TGAATTCCAA AGTCTTGAAT GGCTCCCAGC GGGTGGTGAT GGATGGTGTG 
ATCTCTGATG ATGAGTGCCA GGAGCTGCAG AGACTGACCA ATGCGGCAGC AACTTCGGGA 
GATGGCTACC GAGGTCAGAC CTCCCCACAC ACCCCAAATG AAAAGTTCTA TGGTGTTACT 
GTCCTCAAAG CTCTCAAGCT CGGGCAGGAA GGAAAAGTTC CTCTGCAGAG TGCCCGCATG 
TACTACAACG TGACAGAGAA GGTGCGGCGC GTCATGGAGT CCTACTTCCG CCTGGACACG 
CCCCTCTATT TCTCTTATTC CCACCTCGTG TGCCGCACTG CAATAGAAGA GTCACAGGCT 
GAGAGGAAGG ACAGTAGCCA CCCCGTCCAC GTGGATAACT GCATCCTGAA TGCCGAAGCC 
CTCATGTGTA TCAAGGAGCC CCCAGCCTAC ACGTTCCGGG ACTACAGCGC CATCCTTTAC 
CTCAATGGCG ACTTCGATGG AGGAAACTTT TACTTCACAG AACTAGATGC CAAGACTGTG 
ACGGCAGAGG TGCAGCCCCA GTGTGGAAGG GCTGTGGGAT TCTCTTCTGG CACTGAGAAC 
CCACATGGAG TGAAGGCTGT CACCAGGGGG CAGCGCTGCG CCATCGCCCT GTGGTTCACG 
CTGGATCCTC GGCACAGTGA GAGAGACAGG GTGCAGGCAG ATGACCTGGT GAAGATGCTG 
TTCAGCCCAG AAGAGGTGGA CCTCCCCCAG GAACAGCCCC TGCCTGACCA GCAGGGCTCG 
CCAGAGCCTG GAGAAGAGTC TCTGTCAGAC AGAGGGTCGC TGCACAAGGA TGAGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h1 ( NM_001286148.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273077.1 Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu17163
Clone ID Related Accession (Same CDS sequence)	NM_001286148.1
Accession Version	NM_001286148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 1 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB617770.1, AK132262.1, AK159505.1, AK010578.1 and CO040878.1. Transcript Variant: This variant (4) uses alternate splice sites in the 3' coding region and differs in the 3' UTR, compared to variant 1. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGA GCGAAAGGAG GCTGCTGGCA GCGATGCTAG CGGTCGCCGC CGCCGCCGCC 
TTACGGGTTG CGGCGGAGTC TGAGCCGGGA TGGGACGTGG CAGCCCCTGA CCTGCTTTAC 
GCAGAGGGGA CCGCGGCCTA CTCGCGCGGG GACTGGCCCG GGGTGGTCCT GAACATGGAG 
CGGGCGCTGC GCTCGCGGGC GGCCCTGCGT GCCCTCCGCC TGCGCTGCCG CACACGCTGT 
GCCACCGAAC TGCCGTGGGC ACCGGACCTG GATCTCGGTC CGGACCCCAG CCTGAGCCAG 
GACCCGGGCG CCGCCGCCCT GCACGACCTG CGCTTCTTCG GAGCCGTGCT GCGCCGTGCC 
GCCTGCCTAC GCCGCTGCCT CGGGCCGCCC TCTGCCCACT TGCTGAGTGA AGAACTGGAC 
CTGGAGTTCA ACAAGCGGAG CCCGTACAAC TACCTGCAGG TCGCCTATTT CAAGATAAAC 
AAGCTGGAGA AAGCTGTGGC TGCGGCACAC ACCTTCTTTG TGGGCAATCC TGAGCACATG 
GAGATGCGGC AGAACCTCGA CTATTACCAA ACCATGTCTG GGGTGAAGGA GGCAGACTTC 
AGGGATCTCG AGGCCAAGCC CCATATGCAT GAGTTTCGGC TGGGGGTACG ACTCTACTCA 
GAGGAGAAGC CACAGGAAGC TGTGCCCCAC CTGGAGGCGG CACTGCAAGA GTACTTTGTG 
GCCGATGAGG AATGCCGTGC CCTCTGCGAA GGGCCCTATG ACTACGACGG CTACAACTAC 
CTAGACTACA GCGCTGACCT CTTCCAGGCC ATCACAGATC ATTACGTCCA GGTCCTCAAC 
TGTAAGCAGA ACTGTGTCAC GGAGCTGGCT TCCCACCCAA GTAGGGAAAA GCCCTTTGAA 
GACTTCCTCC CTTCACACTA TAATTACCTA CAGTTTGCCT ACTACAACAT TGGGAACTAT 
ACACAAGCTA TTGAATGTGC CAAGACCTAC CTCCTCTTCT TTCCCAATGA TGAGGTGATG 
CACCAGAATC TGGCTTATTA CACAGCCATG CTTGGAGAAG AAGAGGCCAG CTCCATCAGC 
CCCAGGGAGA ATGCCGAGGA ATACCGACGT CGAAGCCTGT TGGAGAAAGA ACTGCTTTTC 
TTCGCTTATG ACATTTTTGG AATTCCCTTT GTGGATCCGG ACTCATGGAC TCCAGAAGAG 
GTGATTCCCA AGAGACTGCA GGAGAAACAG AATCAGTGCT GTCATGCTCA CAGGTCGGAA 
CGCGAAACCG CAGTCCGCAT CTCCCAGGAG ATTGGCAACC TTATGAAGGA GATCGAGACC 
CTCGTGGAGG AGAAGACCAA GGAGTCCTTG GATGTGAGCC GACTGACGCG GGAAGGTGGT 
CCCCTGCTGT ATGAAGGCAT CAGTCTCACC ATGAATTCCA AAGTCTTGAA TGGCTCCCAG 
CGGGTGGTGA TGGATGGTGT GATCTCTGAT GATGAGTGCC AGGAGCTGCA GAGACTGACC 
AATGCGGCAG CAACTTCGGG AGATGGCTAC CGAGGTCAGA CCTCCCCACA CACCCCAAAT 
GAAAAGTTCT ATGGTGTTAC TGTCCTCAAA GCTCTCAAGC TCGGGCAGGA AGGAAAAGTT 
CCTCTGCAGA GTGCCCGCAT GTACTACAAC GTGACAGAGA AGGTGCGGCG CGTCATGGAG 
TCCTACTTCC GCCTGGACAC GCCCCTCTAT TTCTCTTATT CCCACCTCGT GTGCCGCACT 
GCAATAGAAG AGTCACAGGC TGAGAGGAAG GACAGTAGCC ACCCCGTCCA CGTGGATAAC 
TGCATCCTGA ATGCCGAAGC CCTCATGTGT ATCAAGGAGC CCCCAGCCTA CACGTTCCGG 
GACTACAGCG CCATCCTTTA CCTCAATGGC GACTTCGATG GAGGAAACTT TTACTTCACA 
GAACTAGATG CCAAGACTGT GACGGCAGAG GTGCAGCCCC AGTGTGGAAG GGCTGTGGGA 
TTCTCTTCTG GCACTGAGAA CCCACATGGA GTGAAGGCTG TCACCAGGGG GCAGCGCTGC 
GCCATCGCCC TGTGGTTCAC GCTGGATCCT CGGCACAGTG AGAGAGACAG GGTGCAGGCA 
GATGACCTGG TGAAGATGCT GTTCAGCCCA GAAGAGGTGG ACCTCCCCCA GGAACAGCCC 
CTGCCTGACC AGCAGGGCTC GCCAGAGCCT GGAGAAGAGT CTCTGTCAGA CAGAGGGTCG 
CTGCACAAGG ATGAGCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273077.1
CDS	43..2283
Translation	MAVSERRLLAAMLAVAAAAALRVAAESEPGWDVAAPDLLYAEGTAAYSRGDWPGVVLNMERALRSRAALRALRLRCRTRCATELPWAPDLDLGPDPSLSQDPGAAALHDLRFFGAVLRRAACLRRCLGPPSAHLLSEELDLEFNKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMRQNLDYYQTMSGVKEADFRDLEAKPHMHEFRLGVRLYSEEKPQEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYVQVLNCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFFPNDEVMHQNLAYYTAMLGEEEASSISPRENAEEYRRRSLLEKELLFFAYDIFGIPFVDPDSWTPEEVIPKRLQEKQNQCCHAHRSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKVLNGSQRVVMDGVISDDECQELQRLTNAAATSGDGYRGQTSPHTPNEKFYGVTVLKALKLGQEGKVPLQSARMYYNVTEKVRRVMESYFRLDTPLYFSYSHLVCRTAIEESQAERKDSSHPVHVDNCILNAEALMCIKEPPAYTFRDYSAILYLNGDFDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSPEEVDLPQEQPLPDQQGSPEPGEESLSDRGSLHKDEL

Target ORF information:

RefSeq Version	NM_001286148.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286148.1

ORF Insert Sequence:

ATGGCGGTGA GCGAAAGGAG GCTGCTGGCA GCGATGCTAG CGGTCGCCGC CGCCGCCGCC 
TTACGGGTTG CGGCGGAGTC TGAGCCGGGA TGGGACGTGG CAGCCCCTGA CCTGCTTTAC 
GCAGAGGGGA CCGCGGCCTA CTCGCGCGGG GACTGGCCCG GGGTGGTCCT GAACATGGAG 
CGGGCGCTGC GCTCGCGGGC GGCCCTGCGT GCCCTCCGCC TGCGCTGCCG CACACGCTGT 
GCCACCGAAC TGCCGTGGGC ACCGGACCTG GATCTCGGTC CGGACCCCAG CCTGAGCCAG 
GACCCGGGCG CCGCCGCCCT GCACGACCTG CGCTTCTTCG GAGCCGTGCT GCGCCGTGCC 
GCCTGCCTAC GCCGCTGCCT CGGGCCGCCC TCTGCCCACT TGCTGAGTGA AGAACTGGAC 
CTGGAGTTCA ACAAGCGGAG CCCGTACAAC TACCTGCAGG TCGCCTATTT CAAGATAAAC 
AAGCTGGAGA AAGCTGTGGC TGCGGCACAC ACCTTCTTTG TGGGCAATCC TGAGCACATG 
GAGATGCGGC AGAACCTCGA CTATTACCAA ACCATGTCTG GGGTGAAGGA GGCAGACTTC 
AGGGATCTCG AGGCCAAGCC CCATATGCAT GAGTTTCGGC TGGGGGTACG ACTCTACTCA 
GAGGAGAAGC CACAGGAAGC TGTGCCCCAC CTGGAGGCGG CACTGCAAGA GTACTTTGTG 
GCCGATGAGG AATGCCGTGC CCTCTGCGAA GGGCCCTATG ACTACGACGG CTACAACTAC 
CTAGACTACA GCGCTGACCT CTTCCAGGCC ATCACAGATC ATTACGTCCA GGTCCTCAAC 
TGTAAGCAGA ACTGTGTCAC GGAGCTGGCT TCCCACCCAA GTAGGGAAAA GCCCTTTGAA 
GACTTCCTCC CTTCACACTA TAATTACCTA CAGTTTGCCT ACTACAACAT TGGGAACTAT 
ACACAAGCTA TTGAATGTGC CAAGACCTAC CTCCTCTTCT TTCCCAATGA TGAGGTGATG 
CACCAGAATC TGGCTTATTA CACAGCCATG CTTGGAGAAG AAGAGGCCAG CTCCATCAGC 
CCCAGGGAGA ATGCCGAGGA ATACCGACGT CGAAGCCTGT TGGAGAAAGA ACTGCTTTTC 
TTCGCTTATG ACATTTTTGG AATTCCCTTT GTGGATCCGG ACTCATGGAC TCCAGAAGAG 
GTGATTCCCA AGAGACTGCA GGAGAAACAG AATCAGTGCT GTCATGCTCA CAGGTCGGAA 
CGCGAAACCG CAGTCCGCAT CTCCCAGGAG ATTGGCAACC TTATGAAGGA GATCGAGACC 
CTCGTGGAGG AGAAGACCAA GGAGTCCTTG GATGTGAGCC GACTGACGCG GGAAGGTGGT 
CCCCTGCTGT ATGAAGGCAT CAGTCTCACC ATGAATTCCA AAGTCTTGAA TGGCTCCCAG 
CGGGTGGTGA TGGATGGTGT GATCTCTGAT GATGAGTGCC AGGAGCTGCA GAGACTGACC 
AATGCGGCAG CAACTTCGGG AGATGGCTAC CGAGGTCAGA CCTCCCCACA CACCCCAAAT 
GAAAAGTTCT ATGGTGTTAC TGTCCTCAAA GCTCTCAAGC TCGGGCAGGA AGGAAAAGTT 
CCTCTGCAGA GTGCCCGCAT GTACTACAAC GTGACAGAGA AGGTGCGGCG CGTCATGGAG 
TCCTACTTCC GCCTGGACAC GCCCCTCTAT TTCTCTTATT CCCACCTCGT GTGCCGCACT 
GCAATAGAAG AGTCACAGGC TGAGAGGAAG GACAGTAGCC ACCCCGTCCA CGTGGATAAC 
TGCATCCTGA ATGCCGAAGC CCTCATGTGT ATCAAGGAGC CCCCAGCCTA CACGTTCCGG 
GACTACAGCG CCATCCTTTA CCTCAATGGC GACTTCGATG GAGGAAACTT TTACTTCACA 
GAACTAGATG CCAAGACTGT GACGGCAGAG GTGCAGCCCC AGTGTGGAAG GGCTGTGGGA 
TTCTCTTCTG GCACTGAGAA CCCACATGGA GTGAAGGCTG TCACCAGGGG GCAGCGCTGC 
GCCATCGCCC TGTGGTTCAC GCTGGATCCT CGGCACAGTG AGAGAGACAG GGTGCAGGCA 
GATGACCTGG TGAAGATGCT GTTCAGCCCA GAAGAGGTGG ACCTCCCCCA GGAACAGCCC 
CTGCCTGACC AGCAGGGCTC GCCAGAGCCT GGAGAAGAGT CTCTGTCAGA CAGAGGGTCG 
CTGCACAAGG ATGAGCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bone matrix hypermineralization in prolyl-3 hydroxylase 1 deficient mice.
Bone8515-22(2016 Apr)
Fratzl-Zelman N,B?chinger HP,Vranka JA,Roschger P,Klaushofer K,Rauch F

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)
Street VA,Kujawa SG,Manichaikul A,Broman KW,Kallman JC,Shilling DJ,Iwata AJ,Robinson LC,Robbins CA,Li J,Liberman MC,Tempel BL

Differential effects of collagen prolyl 3-hydroxylation on skeletal tissues.
PLoS genetics10(1)e1004121(2014 Jan)
Homan EP,Lietman C,Grafe I,Lennington J,Morello R,Napierala D,Jiang MM,Munivez EM,Dawson B,Bertin TK,Chen Y,Lua R,Lichtarge O,Hicks J,Weis MA,Eyre D,Lee BH

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
The Journal of biological chemistry288(44)31437-46(2013 Nov)
Ishikawa Y,B?chinger HP

Posttranslational modifications in type I collagen from different tissues extracted from wild type and prolyl 3-hydroxylase 1 null mice.
The Journal of biological chemistry288(34)24742-52(2013 Aug)
Pokidysheva E,Zientek KD,Ishikawa Y,Mizuno K,Vranka JA,Montgomery NT,Keene DR,Kawaguchi T,Okuyama K,B?chinger HP

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P3h1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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