Nrbf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Nrbf2
Entrez Gene ID	641340
Full Name	nuclear receptor binding factor 2
Synonyms	NRBF-2
General protein information	Preferred Names nuclear receptor binding factor 2 Names nuclear receptor-binding factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B5.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314049.1	XP_017169538.1	nuclear receptor-binding factor 2 isoform X1
XM_006513937.3	XP_006514000.1	nuclear receptor-binding factor 2 isoform X2
XM_006513938.3	XP_006514001.1	nuclear receptor-binding factor 2 isoform X3
XM_017314049.2	XP_017169538.1	nuclear receptor-binding factor 2 isoform X1
XM_006513937.4	XP_006514000.1	nuclear receptor-binding factor 2 isoform X2
XM_006513938.4	XP_006514001.1	nuclear receptor-binding factor 2 isoform X3
NM_001036293.2	NP_001031370.1	nuclear receptor-binding factor 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NRBF2	XP_507815.1
Bos taurus (cattle)	NRBF2	NP_001071599.1
Gallus gallus (chicken)	NRBF2	NP_001186462.1
Homo sapiens (human)	NRBF2	NP_110386.2
Xenopus tropicalis (tropical clawed frog)	nrbf2	NP_001016183.1
Rattus norvegicus (Norway rat)	Nrbf2	NP_071522.1
Mus musculus (house mouse)	Nrbf2	NP_001031370.1
Rattus norvegicus (Norway rat)	LOC100363472	XP_002728120.1
	NRBF2	XP_001092137.1
Canis lupus familiaris (dog)	NRBF2	XP_536362.3
Rattus norvegicus (Norway rat)	RGD1560263	XP_574989.1
Danio rerio (zebrafish)	nrbf2b	NP_001002613.1

Gene Ontology
Bibliography

Related articles in PubMed

MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy.
Ma X, Zhang S, He L, Rong Y, Brier LW, Sun Q, Liu R, Fan W, Chen S, Yue Z, Kim J, Guan KL, Li D, Zhong Q
Autophagy13(3)592-607(2017 Mar)

NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity.
Lu J, He L, Behrends C, Araki M, Araki K, Jun Wang Q, Catanzaro JM, Friedman SL, Zong WX, Fiel MI, Li M, Yue Z
Nature communications53920(2014 May)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Nuclear receptor binding factor-2 (NRBF-2), a possible gene activator protein interacting with nuclear hormone receptors.
Yasumo H, Masuda N, Furusawa T, Tsukamoto T, Sadano H, Osumi T
Biochimica et biophysica acta1490(1-2)189-97(2000 Jan)

Exchangeable gene trap using the Cre/mutated lox system.
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reveals NRBF2 as a critical molecular switch of PtdIns3K and autophagy activation, and its on/off state is precisely controlled by MTORC1 through phosphorylation.
Title: MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy.

These data reveal a key role for NRBF2 in the assembly of the specific Atg14L-Beclin 1-Vps34-Vps15 complex for autophagy induction.
Title: NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity.

The following Nrbf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nrbf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12812	NM_001036293.2 Latest version!	Mus musculus nuclear receptor binding factor 2 (Nrbf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu44686	XM_006513937.4 Latest version!	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu44687	XM_006513938.4 Latest version!	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu75525	XM_017314049.2 Latest version!	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu75525	XM_017314049.1	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu44686	XM_006513937.3	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu44687	XM_006513938.3	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nrbf2 ( NM_001036293.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001031370.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), mRNA.

Price & Availability↑

CloneID	OMu12812
Clone ID Related Accession (Same CDS sequence)	NM_001036293.2
Accession Version	NM_001036293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC155712.8. On Nov 17, 2006 this sequence version replaced NM_001036293.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC019448.1, AK002810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGTGA TGGAAGGACC CCTCAACCTT GCTCACCAGC AGAGCCGCCG AGCAGACCGT 
TTGTTAGCTG CTGGGAAATA CGAAGAGGCG ATTTCTTGTC ACAGAAAGGC AACAACATAT 
CTCTCTGAAG CCATGAAGCT GACCGAGTCT GAGCAGGCTC ACTTATCACT GGAACTGCAA 
AGGGACAGCC ACATGAAACA GCTGCTTCTC ATCCAGGAGA GGTGGAAGAG AGCAAAGCGA 
GAGGAGCGGT TGAAAGCGCA GCAGAGCACG GAACGGGACG GAGCCCCCCA CCTGCAGGCA 
CCTCCCAGGC CCTCTGAGGA TGCTGAAGGC CAGAGCCCTC TGCTTTCTCA GCCATACATC 
CCTTCCACAG AGAGGCGCCT CCCCGAGGTG CAGGGGGTCT TTGACAGGGA TCCAGACACA 
TTGCTGTTTT TACTTCAGCA AAAGAATGAG CCATCAGAGC CCTGTATTGG AAGCAAAGCC 
CCAAAAGATG ATAAAACGAT TATAGAGGAG CAGGCAACCA AAATAGCAGA TTTGAAGAGG 
CATGTGGAGT TCCTTGTGGC TGAGAATGAA AGATTAAGGA AAGAAAATAA ACAGCTAAAG 
GCTGAAAAGG CCAGACTCCT GAAAGGCACA GCTGAGAAGG AACTGGACGT CGATGCAGAC 
TTTGTAGAGA AGTCAGAGCT GTGGGGCTTA CCTTCACACT CGGAATCCGC CGCTGCCTCT 
TCAACCTGGC AGAAGTTTGC AGCTAACACG GGGAAAGCCA AGGACATTCC AATACCCAAC 
CTTCCTCCTC TGGATTTCCC ATCTCCAGAG CTTCCTCTGA TGGAGCTCTC GGAGGACATT 
CTGAAAGGAT TCATGAATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031370.1
CDS	156..1019
Translation	MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHRKATTYLSEAMKLTESEQAHLSLELQRDSHMKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	NM_001036293.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036293.2

ORF Insert Sequence:

ATGGAAGTGA TGGAAGGACC CCTCAACCTT GCTCACCAGC AGAGCCGCCG AGCAGACCGT 
TTGTTAGCTG CTGGGAAATA CGAAGAGGCG ATTTCTTGTC ACAGAAAGGC AACAACATAT 
CTCTCTGAAG CCATGAAGCT GACCGAGTCT GAGCAGGCTC ACTTATCACT GGAACTGCAA 
AGGGACAGCC ACATGAAACA GCTGCTTCTC ATCCAGGAGA GGTGGAAGAG AGCAAAGCGA 
GAGGAGCGGT TGAAAGCGCA GCAGAGCACG GAACGGGACG GAGCCCCCCA CCTGCAGGCA 
CCTCCCAGGC CCTCTGAGGA TGCTGAAGGC CAGAGCCCTC TGCTTTCTCA GCCATACATC 
CCTTCCACAG AGAGGCGCCT CCCCGAGGTG CAGGGGGTCT TTGACAGGGA TCCAGACACA 
TTGCTGTTTT TACTTCAGCA AAAGAATGAG CCATCAGAGC CCTGTATTGG AAGCAAAGCC 
CCAAAAGATG ATAAAACGAT TATAGAGGAG CAGGCAACCA AAATAGCAGA TTTGAAGAGG 
CATGTGGAGT TCCTTGTGGC TGAGAATGAA AGATTAAGGA AAGAAAATAA ACAGCTAAAG 
GCTGAAAAGG CCAGACTCCT GAAAGGCACA GCTGAGAAGG AACTGGACGT CGATGCAGAC 
TTTGTAGAGA AGTCAGAGCT GTGGGGCTTA CCTTCACACT CGGAATCCGC CGCTGCCTCT 
TCAACCTGGC AGAAGTTTGC AGCTAACACG GGGAAAGCCA AGGACATTCC AATACCCAAC 
CTTCCTCCTC TGGATTTCCC ATCTCCAGAG CTTCCTCTGA TGGAGCTCTC GGAGGACATT 
CTGAAAGGAT TCATGAATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrbf2 ( XM_006513937.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514000.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44686
Clone ID Related Accession (Same CDS sequence)	XM_006513937.3 , XM_006513937.4
Accession Version	XM_006513937.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514000.1
CDS	126..797
Translation	MKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	XM_006513937.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513937.3

ORF Insert Sequence:

ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrbf2 ( XM_006513938.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514001.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu44687
Clone ID Related Accession (Same CDS sequence)	XM_006513938.3 , XM_006513938.4
Accession Version	XM_006513938.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCGG GCGCCGCCGC TCCGCCTCGG GCCGCTGCTC ACCCCGGGGT TTATGGAAGT 
GATGGAAGGA CCCCTCAACC TTCATATCTC TCTGAAGCCA TGAAGCTGAC CGAGTCTGAG 
CAGGCTCACT TATCACTGGA ACTGCAAAGG GACAGCCACA TGAAACAGCT GCTTCTCATC 
CAGGAGAGGT GGAAGAGAGC AAAGCGAGAG GAGCGGTTGA AAGCGCAGCA GAGCACGGAA 
CGGGACGGAG CCCCCCACCT GCAGGCACCT CCCAGGCCCT CTGAGGATGC TGAAGGCCAG 
AGCCCTCTGC TTTCTCAGCC ATACATCCCT TCCACAGAGA GGCGCCTCCC CGAGGTGCAG 
GGGGTCTTTG ACAGGGATCC AGACACATTG CTGTTTTTAC TTCAGCAAAA GAATGAGCCA 
TCAGAGCCCT GTATTGGAAG CAAAGCCCCA AAAGATGATA AAACGATTAT AGAGGAGCAG 
GCAACCAAAA TAGCAGATTT GAAGAGGCAT GTGGAGTTCC TTGTGGCTGA GAATGAAAGA 
TTAAGGAAAG AAAATAAACA GCTAAAGGCT GAAAAGGCCA GACTCCTGAA AGGCACAGCT 
GAGAAGGAAC TGGACGTCGA TGCAGACTTT GTAGAGAAGT CAGAGCTGTG GGGCTTACCT 
TCACACTCGG AATCCGCCGC TGCCTCTTCA ACCTGGCAGA AGTTTGCAGC TAACACGGGG 
AAAGCCAAGG ACATTCCAAT ACCCAACCTT CCTCCTCTGG ATTTCCCATC TCCAGAGCTT 
CCTCTGATGG AGCTCTCGGA GGACATTCTG AAAGGATTCA TGAATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514001.1
CDS	168..998
Translation	MCPGAAAPPRAAAHPGVYGSDGRTPQPSYLSEAMKLTESEQAHLSLELQRDSHMKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	XM_006513938.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513938.3

ORF Insert Sequence:

ATGTGCCCGG GCGCCGCCGC TCCGCCTCGG GCCGCTGCTC ACCCCGGGGT TTATGGAAGT 
GATGGAAGGA CCCCTCAACC TTCATATCTC TCTGAAGCCA TGAAGCTGAC CGAGTCTGAG 
CAGGCTCACT TATCACTGGA ACTGCAAAGG GACAGCCACA TGAAACAGCT GCTTCTCATC 
CAGGAGAGGT GGAAGAGAGC AAAGCGAGAG GAGCGGTTGA AAGCGCAGCA GAGCACGGAA 
CGGGACGGAG CCCCCCACCT GCAGGCACCT CCCAGGCCCT CTGAGGATGC TGAAGGCCAG 
AGCCCTCTGC TTTCTCAGCC ATACATCCCT TCCACAGAGA GGCGCCTCCC CGAGGTGCAG 
GGGGTCTTTG ACAGGGATCC AGACACATTG CTGTTTTTAC TTCAGCAAAA GAATGAGCCA 
TCAGAGCCCT GTATTGGAAG CAAAGCCCCA AAAGATGATA AAACGATTAT AGAGGAGCAG 
GCAACCAAAA TAGCAGATTT GAAGAGGCAT GTGGAGTTCC TTGTGGCTGA GAATGAAAGA 
TTAAGGAAAG AAAATAAACA GCTAAAGGCT GAAAAGGCCA GACTCCTGAA AGGCACAGCT 
GAGAAGGAAC TGGACGTCGA TGCAGACTTT GTAGAGAAGT CAGAGCTGTG GGGCTTACCT 
TCACACTCGG AATCCGCCGC TGCCTCTTCA ACCTGGCAGA AGTTTGCAGC TAACACGGGG 
AAAGCCAAGG ACATTCCAAT ACCCAACCTT CCTCCTCTGG ATTTCCCATC TCCAGAGCTT 
CCTCTGATGG AGCTCTCGGA GGACATTCTG AAAGGATTCA TGAATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrbf2 ( XM_017314049.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169538.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu75525
Clone ID Related Accession (Same CDS sequence)	XM_017314049.1 , XM_017314049.2
Accession Version	XM_017314049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA CCGAGTCTGA GCAGGCTCAC TTATCACTGG AACTGCAAAG GGACAGCCAC 
ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169538.1
CDS	328..1059
Translation	MKLTESEQAHLSLELQRDSHMKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	XM_017314049.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314049.1

ORF Insert Sequence:

ATGAAGCTGA CCGAGTCTGA GCAGGCTCAC TTATCACTGG AACTGCAAAG GGACAGCCAC 
ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrbf2 ( XM_006513937.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514000.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44686
Clone ID Related Accession (Same CDS sequence)	XM_006513937.3 , XM_006513937.4
Accession Version	XM_006513937.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513937.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514000.1
CDS	158..829
Translation	MKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	XM_006513937.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513937.4

ORF Insert Sequence:

ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrbf2 ( XM_006513938.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514001.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu44687
Clone ID Related Accession (Same CDS sequence)	XM_006513938.3 , XM_006513938.4
Accession Version	XM_006513938.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513938.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCCGG GCGCCGCCGC TCCGCCTCGG GCCGCTGCTC ACCCCGGGGT TTATGGAAGT 
GATGGAAGGA CCCCTCAACC TTCATATCTC TCTGAAGCCA TGAAGCTGAC CGAGTCTGAG 
CAGGCTCACT TATCACTGGA ACTGCAAAGG GACAGCCACA TGAAACAGCT GCTTCTCATC 
CAGGAGAGGT GGAAGAGAGC AAAGCGAGAG GAGCGGTTGA AAGCGCAGCA GAGCACGGAA 
CGGGACGGAG CCCCCCACCT GCAGGCACCT CCCAGGCCCT CTGAGGATGC TGAAGGCCAG 
AGCCCTCTGC TTTCTCAGCC ATACATCCCT TCCACAGAGA GGCGCCTCCC CGAGGTGCAG 
GGGGTCTTTG ACAGGGATCC AGACACATTG CTGTTTTTAC TTCAGCAAAA GAATGAGCCA 
TCAGAGCCCT GTATTGGAAG CAAAGCCCCA AAAGATGATA AAACGATTAT AGAGGAGCAG 
GCAACCAAAA TAGCAGATTT GAAGAGGCAT GTGGAGTTCC TTGTGGCTGA GAATGAAAGA 
TTAAGGAAAG AAAATAAACA GCTAAAGGCT GAAAAGGCCA GACTCCTGAA AGGCACAGCT 
GAGAAGGAAC TGGACGTCGA TGCAGACTTT GTAGAGAAGT CAGAGCTGTG GGGCTTACCT 
TCACACTCGG AATCCGCCGC TGCCTCTTCA ACCTGGCAGA AGTTTGCAGC TAACACGGGG 
AAAGCCAAGG ACATTCCAAT ACCCAACCTT CCTCCTCTGG ATTTCCCATC TCCAGAGCTT 
CCTCTGATGG AGCTCTCGGA GGACATTCTG AAAGGATTCA TGAATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514001.1
CDS	1..831
Translation	MCPGAAAPPRAAAHPGVYGSDGRTPQPSYLSEAMKLTESEQAHLSLELQRDSHMKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	XM_006513938.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513938.4

ORF Insert Sequence:

ATGTGCCCGG GCGCCGCCGC TCCGCCTCGG GCCGCTGCTC ACCCCGGGGT TTATGGAAGT 
GATGGAAGGA CCCCTCAACC TTCATATCTC TCTGAAGCCA TGAAGCTGAC CGAGTCTGAG 
CAGGCTCACT TATCACTGGA ACTGCAAAGG GACAGCCACA TGAAACAGCT GCTTCTCATC 
CAGGAGAGGT GGAAGAGAGC AAAGCGAGAG GAGCGGTTGA AAGCGCAGCA GAGCACGGAA 
CGGGACGGAG CCCCCCACCT GCAGGCACCT CCCAGGCCCT CTGAGGATGC TGAAGGCCAG 
AGCCCTCTGC TTTCTCAGCC ATACATCCCT TCCACAGAGA GGCGCCTCCC CGAGGTGCAG 
GGGGTCTTTG ACAGGGATCC AGACACATTG CTGTTTTTAC TTCAGCAAAA GAATGAGCCA 
TCAGAGCCCT GTATTGGAAG CAAAGCCCCA AAAGATGATA AAACGATTAT AGAGGAGCAG 
GCAACCAAAA TAGCAGATTT GAAGAGGCAT GTGGAGTTCC TTGTGGCTGA GAATGAAAGA 
TTAAGGAAAG AAAATAAACA GCTAAAGGCT GAAAAGGCCA GACTCCTGAA AGGCACAGCT 
GAGAAGGAAC TGGACGTCGA TGCAGACTTT GTAGAGAAGT CAGAGCTGTG GGGCTTACCT 
TCACACTCGG AATCCGCCGC TGCCTCTTCA ACCTGGCAGA AGTTTGCAGC TAACACGGGG 
AAAGCCAAGG ACATTCCAAT ACCCAACCTT CCTCCTCTGG ATTTCCCATC TCCAGAGCTT 
CCTCTGATGG AGCTCTCGGA GGACATTCTG AAAGGATTCA TGAATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrbf2 ( XM_017314049.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169538.1 Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu75525
Clone ID Related Accession (Same CDS sequence)	XM_017314049.1 , XM_017314049.2
Accession Version	XM_017314049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear receptor-binding factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA CCGAGTCTGA GCAGGCTCAC TTATCACTGG AACTGCAAAG GGACAGCCAC 
ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169538.1
CDS	575..1306
Translation	MKLTESEQAHLSLELQRDSHMKQLLLIQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDTLLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGTAEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMND

Target ORF information:

RefSeq Version	XM_017314049.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor binding factor 2 (Nrbf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314049.2

ORF Insert Sequence:

ATGAAGCTGA CCGAGTCTGA GCAGGCTCAC TTATCACTGG AACTGCAAAG GGACAGCCAC 
ATGAAACAGC TGCTTCTCAT CCAGGAGAGG TGGAAGAGAG CAAAGCGAGA GGAGCGGTTG 
AAAGCGCAGC AGAGCACGGA ACGGGACGGA GCCCCCCACC TGCAGGCACC TCCCAGGCCC 
TCTGAGGATG CTGAAGGCCA GAGCCCTCTG CTTTCTCAGC CATACATCCC TTCCACAGAG 
AGGCGCCTCC CCGAGGTGCA GGGGGTCTTT GACAGGGATC CAGACACATT GCTGTTTTTA 
CTTCAGCAAA AGAATGAGCC ATCAGAGCCC TGTATTGGAA GCAAAGCCCC AAAAGATGAT 
AAAACGATTA TAGAGGAGCA GGCAACCAAA ATAGCAGATT TGAAGAGGCA TGTGGAGTTC 
CTTGTGGCTG AGAATGAAAG ATTAAGGAAA GAAAATAAAC AGCTAAAGGC TGAAAAGGCC 
AGACTCCTGA AAGGCACAGC TGAGAAGGAA CTGGACGTCG ATGCAGACTT TGTAGAGAAG 
TCAGAGCTGT GGGGCTTACC TTCACACTCG GAATCCGCCG CTGCCTCTTC AACCTGGCAG 
AAGTTTGCAG CTAACACGGG GAAAGCCAAG GACATTCCAA TACCCAACCT TCCTCCTCTG 
GATTTCCCAT CTCCAGAGCT TCCTCTGATG GAGCTCTCGG AGGACATTCT GAAAGGATTC 
ATGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MTORC1-mediated NRBF2 phosphorylation functions as a switch for the class III PtdIns3K and autophagy.
Autophagy13(3)592-607(2017 Mar)
Ma X,Zhang S,He L,Rong Y,Brier LW,Sun Q,Liu R,Fan W,Chen S,Yue Z,Kim J,Guan KL,Li D,Zhong Q

NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity.
Nature communications53920(2014 May)
Lu J,He L,Behrends C,Araki M,Araki K,Jun Wang Q,Catanzaro JM,Friedman SL,Zong WX,Fiel MI,Li M,Yue Z

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Nuclear receptor binding factor-2 (NRBF-2), a possible gene activator protein interacting with nuclear hormone receptors.
Biochimica et biophysica acta1490(1-2)189-97(2000 Jan)
Yasumo H,Masuda N,Furusawa T,Tsukamoto T,Sadano H,Osumi T

Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)
Araki K,Imaizumi T,Sekimoto T,Yoshinobu K,Yoshimuta J,Akizuki M,Miura K,Araki M,Yamamura K

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Nrbf2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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