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Iscu cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Iscu
Entrez Gene ID 66383
Full Name iron-sulfur cluster assembly enzyme
Synonyms 2310020H20Rik,AA407971,Nifu,Nifun
General protein information
Preferred Names

iron-sulfur cluster assembly enzyme

Names

iron-sulfur cluster assembly enzyme ISCU, mitochondrial
ISCU2
IscU iron-sulfur cluster scaffold homolog
NifU-like N-terminal domain containing
nifU-like N-terminal domain-containing protein
nifU-like protein
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

5

5|5 F

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_025526.4 NP_079802.1 iron-sulfur cluster assembly enzyme ISCU, mitochondrial precursor
NM_001363317.1 NP_001350246.1 iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 2
XM_030254754.1 XP_030110614.1 iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X1
XM_030254755.1 XP_030110615.1 iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X2
NM_025526.5 NP_079802.1 iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 1 precursor

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) ISCU XP_509343.2
Canis lupus familiaris (dog) LOC477527 XP_534722.1
Mus musculus (house mouse) Iscu NP_079802.1
Drosophila melanogaster (fruit fly) CG9836 NP_649840.1
Oryza sativa (rice) Os01g0662600 NP_001043783.1
Oryza sativa (rice) Os05g0568100 NP_001056355.1
Xenopus tropicalis (tropical clawed frog) iscu NP_989088.1
Bos taurus (cattle) ISCU NP_001069151.1
Danio rerio (zebrafish) iscub NP_001017800.2
Caenorhabditis elegans (roundworm) Y45F10D.4 NP_502658.1
Saccharomyces cerevisiae (baker's yeast) ISU1 NP_015190.1
Danio rerio (zebrafish) si:ch211-191d15.2 NP_001003632.1
Saccharomyces cerevisiae (baker's yeast) ISU2 NP_014869.3
Eremothecium gossypii AGOS_ACR112C NP_983514.1
Neurospora crassa NCU05778 XP_960038.1
Homo sapiens (human) ISCU NP_998760.1
Rattus norvegicus (Norway rat) Iscu NP_001099406.1
Gallus gallus (chicken) ISCU XP_003642230.2
Arabidopsis thaliana (thale cress) ISU1 NP_193953.1
Arabidopsis thaliana (thale cress) ISU2 NP_186751.1
ISCU XP_001103180.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005813 XP_315834.2
Kluyveromyces lactis KLLA0D07161g XP_453380.1
Schizosaccharomyces pombe (fission yeast) isu1 NP_592967.1
Arabidopsis thaliana (thale cress) ISU3 NP_192317.1

Gene Ontology


Bibliography

Related articles in PubMed

Regulation of iron homeostasis by the p53-ISCU pathway.
Funauchi Y, Tanikawa C, Yi Lo PH, Mori J, Daigo Y, Takano A, Miyagi Y, Okawa A, Nakamura Y, Matsuda K
Scientific reports516497(2015 Nov)

The role of zinc in the stability of the marginally stable IscU scaffold protein.
Iannuzzi C, Adrover M, Puglisi R, Yan R, Temussi PA, Pastore A
Protein science : a publication of the Protein Society23(9)1208-19(2014 Sep)

Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.
La P, Yang G, Dennery PA
The Journal of biological chemistry288(18)12901-9(2013 May)

Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Nordin A, Larsson E, Thornell LE, Holmberg M
Human genetics129(4)371-8(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ISCU expression was decreased in the majority of human liver cancer tissues, and its reduced expression was significantly associated with p53 mutation.
Title: Regulation of iron homeostasis by the p53-ISCU pathway.

IscU is a marginally stable protein at low ionic strength to the point that undergoes cold denaturation at around -8 degrees C with a corresponding dramatic decrease of enthalpy, which is consistent with the fluxional nature of the protein.
Title: The role of zinc in the stability of the marginally stable IscU scaffold protein.

mTORC1 associates with ISCU and phosphorylates ISCU at serine 14. This phosphorylation stabilized ISCU protein.
Title: Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.

Data show that complete loss of ISCU results in early embryonic death and confirm a fundamental role for ISCU in mammals.
Title: Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.

Results identify the iron-sulfur cluster assembly proteins (ISCU1/2) as direct targets for repression by the hypoxia-induced microRNA-210.
Title: MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2.

The following Iscu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iscu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu23073 NM_025526.5
Latest version!		 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $69.30
$99.00
OMu84275 NM_001363317.1
Latest version!		 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OMu88994 XM_030254754.1
Latest version!		 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu88995 XM_030254755.1
Latest version!		 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu23073 NM_025526.4 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu23073
Clone ID Related Accession (Same CDS sequence) NM_025526.4 , NM_025526.5
Accession Version NM_025526.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 507bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Mus musculus(house mouse)
Product iron-sulfur cluster assembly enzyme ISCU, mitochondrial precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132939.23. On Oct 19, 2007 this sequence version replaced NM_025526.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK009021.1, AK083247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_079802.1
CDS17..523
Translation

Target ORF information:

RefSeq Version NM_025526.4
Organism Mus musculus(house mouse)
Definition Mus musculus iron-sulfur cluster assembly enzyme (Iscu), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_025526.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23073
Clone ID Related Accession (Same CDS sequence) NM_025526.4 , NM_025526.5
Accession Version NM_025526.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 507bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-30
Organism Mus musculus(house mouse)
Product iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 1 precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132939.23. On May 17, 2018 this sequence version replaced NM_025526.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CK791593.1, AK039834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_079802.1
CDS28..534
Translation

Target ORF information:

RefSeq Version NM_025526.5
Organism Mus musculus(house mouse)
Definition Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_025526.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu84275
Clone ID Related Accession (Same CDS sequence) NM_001363317.1
Accession Version NM_001363317.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-05
Organism Mus musculus(house mouse)
Product iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132939.23, AI851358.1 and AK083247.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AI851358.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCATGCTGG CAGAAGACGC CATCAAGGCC 
GCCCTGGCTG ACTACAAACT GAAGCAAGAG TCCAAGAAGG AGGAGCCAGA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350246.1
CDS147..686
Translation

Target ORF information:

RefSeq Version NM_001363317.1
Organism Mus musculus(house mouse)
Definition Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363317.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCATGCTGG CAGAAGACGC CATCAAGGCC 
GCCCTGGCTG ACTACAAACT GAAGCAAGAG TCCAAGAAGG AGGAGCCAGA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu88994
Clone ID Related Accession (Same CDS sequence) XM_030254754.1
Accession Version XM_030254754.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGCATGATC GAATCATAAA CACGCCTGAA ACCGAGCATC CCCACGCCCA TCGCAGCACC 
AGACAACCTT GCACGCCGCT TCGGACCTTC CCGCTCTGCG CCTGGCCACG CCCCTTCCGA 
GGGCGCGGTC AGAGGGGGCG TAGCCGGGCC GAGACGCCAG CGCAAGCGCC GAGCTGCGGT 
GACGTCGAAC GGGCGCGCCA TCCCTCCGGC GTCGCCGAGG TCGACGCAGC TCAAGCCGGC 
AGGATGGCGG CGGCCACGGG AGCTGGCCGT CTGAGGCGGG CGGCGTCGGC GCTGCTGCTG 
CGGAGCCCGC GCCTGCCCGC CCGGGAGCTG TCGGCTCCGG CCAGGCTCTA CCACAAGAAG 
GTTGTGGATC ATTATGAAAA CCCTCGGAAC GTGGGATCCC TTGACAAGAC ATCTAAAAAT 
GTTGGAACCG GATTGGTGGG GGCTCCGGCA TGTGGTGACG TCATGAAACT GCAGATCCAG 
GTGGATGAAA AGGGGAAGAT TGTGGACGCC AGATTCAAAA CATTTGGCTG CGGCTCCGCC 
ATTGCCTCCA GCTCCTTAGC CACAGAGTGG GTAAAGGGGA AAACGGTGGA GGAAGCCCTG 
ACCATCAAAA ACACCGACAT CGCCAAGGAG CTCTGCCTGC CGCCTGTGAA ACTGCACTGC 
TCCAGTGAGT CTGCCCCACC TGCCAGGCAG CTTGGATCTG TTCAGAGTCT GCTGTTCCCA 
CCCCACGGTG GCCGGGCAGC TGAGACAGGC AGCTCTTCCC CCACCCTGCG CTTCCCCAGT 
CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA GTGCCTGAGG CCACAGTGCA CTACCTACCA 
CACTGCCCCA CGAGAGCCTT CCCACACTGC TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030110614.1
CDS168..1040
Translation

Target ORF information:

RefSeq Version XM_030254754.1
Organism Mus musculus(house mouse)
Definition Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030254754.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGCATGATC GAATCATAAA CACGCCTGAA ACCGAGCATC CCCACGCCCA TCGCAGCACC 
AGACAACCTT GCACGCCGCT TCGGACCTTC CCGCTCTGCG CCTGGCCACG CCCCTTCCGA 
GGGCGCGGTC AGAGGGGGCG TAGCCGGGCC GAGACGCCAG CGCAAGCGCC GAGCTGCGGT 
GACGTCGAAC GGGCGCGCCA TCCCTCCGGC GTCGCCGAGG TCGACGCAGC TCAAGCCGGC 
AGGATGGCGG CGGCCACGGG AGCTGGCCGT CTGAGGCGGG CGGCGTCGGC GCTGCTGCTG 
CGGAGCCCGC GCCTGCCCGC CCGGGAGCTG TCGGCTCCGG CCAGGCTCTA CCACAAGAAG 
GTTGTGGATC ATTATGAAAA CCCTCGGAAC GTGGGATCCC TTGACAAGAC ATCTAAAAAT 
GTTGGAACCG GATTGGTGGG GGCTCCGGCA TGTGGTGACG TCATGAAACT GCAGATCCAG 
GTGGATGAAA AGGGGAAGAT TGTGGACGCC AGATTCAAAA CATTTGGCTG CGGCTCCGCC 
ATTGCCTCCA GCTCCTTAGC CACAGAGTGG GTAAAGGGGA AAACGGTGGA GGAAGCCCTG 
ACCATCAAAA ACACCGACAT CGCCAAGGAG CTCTGCCTGC CGCCTGTGAA ACTGCACTGC 
TCCAGTGAGT CTGCCCCACC TGCCAGGCAG CTTGGATCTG TTCAGAGTCT GCTGTTCCCA 
CCCCACGGTG GCCGGGCAGC TGAGACAGGC AGCTCTTCCC CCACCCTGCG CTTCCCCAGT 
CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA GTGCCTGAGG CCACAGTGCA CTACCTACCA 
CACTGCCCCA CGAGAGCCTT CCCACACTGC TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu88995
Clone ID Related Accession (Same CDS sequence) XM_030254755.1
Accession Version XM_030254755.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 663bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCAGTGAGT CTGCCCCACC TGCCAGGCAG 
CTTGGATCTG TTCAGAGTCT GCTGTTCCCA CCCCACGGTG GCCGGGCAGC TGAGACAGGC 
AGCTCTTCCC CCACCCTGCG CTTCCCCAGT CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA 
GTGCCTGAGG CCACAGTGCA CTACCTACCA CACTGCCCCA CGAGAGCCTT CCCACACTGC 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030110615.1
CDS177..839
Translation

Target ORF information:

RefSeq Version XM_030254755.1
Organism Mus musculus(house mouse)
Definition Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030254755.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCAGTGAGT CTGCCCCACC TGCCAGGCAG 
CTTGGATCTG TTCAGAGTCT GCTGTTCCCA CCCCACGGTG GCCGGGCAGC TGAGACAGGC 
AGCTCTTCCC CCACCCTGCG CTTCCCCAGT CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA 
GTGCCTGAGG CCACAGTGCA CTACCTACCA CACTGCCCCA CGAGAGCCTT CCCACACTGC 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Regulation of iron homeostasis by the p53-ISCU pathway.
Scientific reports516497(2015 Nov)
Funauchi Y,Tanikawa C,Yi Lo PH,Mori J,Daigo Y,Takano A,Miyagi Y,Okawa A,Nakamura Y,Matsuda K


book

The role of zinc in the stability of the marginally stable IscU scaffold protein.
Protein science : a publication of the Protein Society23(9)1208-19(2014 Sep)
Iannuzzi C,Adrover M,Puglisi R,Yan R,Temussi PA,Pastore A


book

Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.
The Journal of biological chemistry288(18)12901-9(2013 May)
La P,Yang G,Dennery PA


book

Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Human genetics129(4)371-8(2011 Apr)
Nordin A,Larsson E,Thornell LE,Holmberg M