B230118H07Rik cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	B230118H07Rik
Entrez Gene ID	68170
Full Name	RIKEN cDNA B230118H07 gene
Synonyms	NWC
General protein information	Preferred Names RIKEN cDNA B230118H07 gene Names uncharacterized protein C11orf74 homolog Rag1/Nwc fusion protein Rag1/Rag2/Nwc fusion protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E2\|2 53.83 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500094.3	XP_006500157.1	uncharacterized protein C11orf74 homolog isoform X1
NM_001362687.1	NP_001349616.1	protein C11orf74 homolog isoform 1
NM_026592.4	NP_080868.1	protein C11orf74 homolog isoform 1
NM_026592.3	NP_080868.1	uncharacterized protein C11orf74 homolog
NM_001362688.1	NP_001349617.1	protein C11orf74 homolog isoform 2
XM_006500095.3	XP_006500158.1	uncharacterized protein C11orf74 homolog isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	B230118H07Rik	NP_080868.1
Homo sapiens (human)	C11orf74	NP_001263652.1
Canis lupus familiaris (dog)	C18H11orf74	XP_533151.3
	C14H11orf74	XP_001114718.1
Bos taurus (cattle)	C15H11orf74	NP_001029922.1
Pan troglodytes (chimpanzee)	C11H11orf74	XP_001155047.1
Rattus norvegicus (Norway rat)	RGD1309730	NP_001099961.2

Gene Ontology
Bibliography

Related articles in PubMed

Search for the Function of NWC, Third Gene Within RAG Locus: Generation and Characterization of NWC-Deficient Mice.
Kasztura M, Sniezewski L, Laszkiewicz A, Majkowski M, Kobak K, Peczek K, Janik S, Kapusniak V, Miazek A, Cebrat M, Kisielow P
Archivum immunologiae et therapiae experimentalis64(4)311-9(2016 Aug)

Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter.
Laszkiewicz A, Bzdzion ?, Kasztura M, Snie?ewski L, Janik S, Kisielow P, Cebrat M
PloS one9(9)e106927(2014)

Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells.
Laszkiewicz A, Sniezewski L, Kasztura M, Bzdzion L, Cebrat M, Kisielow P
PloS one7(9)e44807(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

functional inactivation of NWC does not cause any gross developmental, physiological or reproductive abnormalities, and that under physiological conditions NWC may be involved in assembling and functioning of cilia
Title: Search for the Function of NWC, Third Gene Within RAG Locus: Generation and Characterization of NWC-Deficient Mice.

Ikaros, a lymphocyte-specific transcription factor, acts as a repressor of NWC promoter--thus identifying a new Ikaros target--but is insufficient for inducing methylation which depends on antisense transcription driven by RAG-2 promoter
Title: Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter.

Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells
Title: Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells.

Careful analysis of the Rag2 intergenic region uncovered a second evolutionarily conserved Nwc promoter region from which a previously unknown Nwc transcript can be generated in nonlymphocytes of Nwctmpro1 and normal mice.
Title: Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron.

These results indicate that accessibility of NWC promoter and RAG promoters to trans-acting factors is regulated by different epigenetic mechanisms.
Title: Mechanism of lymphocyte-specific inactivation of RAG-2 intragenic promoter of NWC: implications for epigenetic control of RAG locus.

The following B230118H07Rik gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B230118H07Rik cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30835	XM_006500095.3 Latest version!	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu15192	NM_026592.4 Latest version!	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu15192	NM_001362687.1 Latest version!	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu30834	XM_006500094.3 Latest version!	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu84317	NM_001362688.1 Latest version!	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu15192	NM_026592.3	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B230118H07Rik ( XM_006500095.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500158.1 Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30835
Clone ID Related Accession (Same CDS sequence)	XM_006500095.3
Accession Version	XM_006500095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	uncharacterized protein C11orf74 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGGG CTGGAAAGGT GAAGGTAGAC AACTTCCTAG ACTTAGAAGA TGTGGACATG 
GAGGAAGAGA GTAAGCCCCA GATGAACAAG GATTGCCTGC TACTTCCAGG TGAGGTGGAA 
CAGGATATAA GCACCAGCGT TCCATCTTAT GATCCTTCTG TGGACCTACC GCTCACCTCT 
GAGGTGAGGC CAAAGCCTGC TGTGGGAAGG ACTCAGAAAC AAACCGAAGA GATACTTGGA 
GATGAAGTTC AGCCCTTCTT TCTCGATGAA GAATTTGATT ATGACAATGT GACTCTCACT 
CCTAAGTTTA CTGCTGGAGA GATAGCAACC ATCCAAGAGC TATCTAAGCA AAAGAGAGTG 
GGTTCCGGTA CAAACGTGTG CGAACTCCAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500158.1
CDS	659..1054
Translation	MTRAGKVKVDNFLDLEDVDMEEESKPQMNKDCLLLPGEVEQDISTSVPSYDPSVDLPLTSEVRPKPAVGRTQKQTEEILGDEVQPFFLDEEFDYDNVTLTPKFTAGEIATIQELSKQKRVGSGTNVCELHD

Target ORF information:

RefSeq Version	XM_006500095.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500095.3

ORF Insert Sequence:

ATGACCCGGG CTGGAAAGGT GAAGGTAGAC AACTTCCTAG ACTTAGAAGA TGTGGACATG 
GAGGAAGAGA GTAAGCCCCA GATGAACAAG GATTGCCTGC TACTTCCAGG TGAGGTGGAA 
CAGGATATAA GCACCAGCGT TCCATCTTAT GATCCTTCTG TGGACCTACC GCTCACCTCT 
GAGGTGAGGC CAAAGCCTGC TGTGGGAAGG ACTCAGAAAC AAACCGAAGA GATACTTGGA 
GATGAAGTTC AGCCCTTCTT TCTCGATGAA GAATTTGATT ATGACAATGT GACTCTCACT 
CCTAAGTTTA CTGCTGGAGA GATAGCAACC ATCCAAGAGC TATCTAAGCA AAAGAGAGTG 
GGTTCCGGTA CAAACGTGTG CGAACTCCAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B230118H07Rik ( NM_001362687.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349616.1 Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15192
Clone ID Related Accession (Same CDS sequence)	NM_001362687.1 , NM_026592.4 , NM_026592.3
Accession Version	NM_001362687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	protein C11orf74 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929569.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK088637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG ATACTTGGAG ATGAAGTTCA GCCCTTCTTT 
CTCGATGAAG AATTTGATTA TGACAATGTG ACTCTCACTC CTAAGTTTAC TGCTGGAGAG 
ATAGCAACCA TCCAAGAGCT ATCTAAGCAA AAGAGAGTGG GTTCCGGTAC AAACGTGTGC 
GAACTCCATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349616.1
CDS	169..903
Translation	MLAQIPRPEIMDEDQLMKEVLDKFVNCHEQTYEEFLSTFTHLSKEDNVAKWGAHGTDSSENIFSTVKWNHKTESNGRHFRNTSIFLHTSSEHSEEDQIVIDEGHKVGSSFQGDMTRAGKVKVDNFLDLEDVDMEEESKPQMNKDCLLLPGEVEQDISTSVPSYDPSVDLPLTSEVRPKPAVGRTQKQTEEILGDEVQPFFLDEEFDYDNVTLTPKFTAGEIATIQELSKQKRVGSGTNVCELHD

Target ORF information:

RefSeq Version	NM_001362687.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362687.1

ORF Insert Sequence:

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG ATACTTGGAG ATGAAGTTCA GCCCTTCTTT 
CTCGATGAAG AATTTGATTA TGACAATGTG ACTCTCACTC CTAAGTTTAC TGCTGGAGAG 
ATAGCAACCA TCCAAGAGCT ATCTAAGCAA AAGAGAGTGG GTTCCGGTAC AAACGTGTGC 
GAACTCCATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B230118H07Rik ( NM_026592.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080868.1 Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15192
Clone ID Related Accession (Same CDS sequence)	NM_001362687.1 , NM_026592.4 , NM_026592.3
Accession Version	NM_026592.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Mus musculus(house mouse)
Product	protein C11orf74 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY777225.1, AK051679.1 and BE946529.1. On Apr 25, 2018 this sequence version replaced NM_026592.3. ##Evidence-Data-START## Transcript exon combination :: AK051679.1, CB233850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG ATACTTGGAG ATGAAGTTCA GCCCTTCTTT 
CTCGATGAAG AATTTGATTA TGACAATGTG ACTCTCACTC CTAAGTTTAC TGCTGGAGAG 
ATAGCAACCA TCCAAGAGCT ATCTAAGCAA AAGAGAGTGG GTTCCGGTAC AAACGTGTGC 
GAACTCCATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080868.1
CDS	171..905
Translation	MLAQIPRPEIMDEDQLMKEVLDKFVNCHEQTYEEFLSTFTHLSKEDNVAKWGAHGTDSSENIFSTVKWNHKTESNGRHFRNTSIFLHTSSEHSEEDQIVIDEGHKVGSSFQGDMTRAGKVKVDNFLDLEDVDMEEESKPQMNKDCLLLPGEVEQDISTSVPSYDPSVDLPLTSEVRPKPAVGRTQKQTEEILGDEVQPFFLDEEFDYDNVTLTPKFTAGEIATIQELSKQKRVGSGTNVCELHD

Target ORF information:

RefSeq Version	NM_026592.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026592.4

ORF Insert Sequence:

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG ATACTTGGAG ATGAAGTTCA GCCCTTCTTT 
CTCGATGAAG AATTTGATTA TGACAATGTG ACTCTCACTC CTAAGTTTAC TGCTGGAGAG 
ATAGCAACCA TCCAAGAGCT ATCTAAGCAA AAGAGAGTGG GTTCCGGTAC AAACGTGTGC 
GAACTCCATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B230118H07Rik ( NM_026592.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080868.1 Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), mRNA.

Price & Availability↑

CloneID	OMu15192
Clone ID Related Accession (Same CDS sequence)	NM_001362687.1 , NM_026592.4 , NM_026592.3
Accession Version	NM_026592.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Mus musculus(house mouse)
Product	uncharacterized protein C11orf74 homolog
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from AK051679.1. On Apr 6, 2007 this sequence version replaced NM_026592.2. ##Evidence-Data-START## Transcript exon combination :: AK051679.1, CB233850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG ATACTTGGAG ATGAAGTTCA GCCCTTCTTT 
CTCGATGAAG AATTTGATTA TGACAATGTG ACTCTCACTC CTAAGTTTAC TGCTGGAGAG 
ATAGCAACCA TCCAAGAGCT ATCTAAGCAA AAGAGAGTGG GTTCCGGTAC AAACGTGTGC 
GAACTCCATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080868.1
CDS	126..860
Translation	MLAQIPRPEIMDEDQLMKEVLDKFVNCHEQTYEEFLSTFTHLSKEDNVAKWGAHGTDSSENIFSTVKWNHKTESNGRHFRNTSIFLHTSSEHSEEDQIVIDEGHKVGSSFQGDMTRAGKVKVDNFLDLEDVDMEEESKPQMNKDCLLLPGEVEQDISTSVPSYDPSVDLPLTSEVRPKPAVGRTQKQTEEILGDEVQPFFLDEEFDYDNVTLTPKFTAGEIATIQELSKQKRVGSGTNVCELHD

Target ORF information:

RefSeq Version	NM_026592.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026592.3

ORF Insert Sequence:

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG ATACTTGGAG ATGAAGTTCA GCCCTTCTTT 
CTCGATGAAG AATTTGATTA TGACAATGTG ACTCTCACTC CTAAGTTTAC TGCTGGAGAG 
ATAGCAACCA TCCAAGAGCT ATCTAAGCAA AAGAGAGTGG GTTCCGGTAC AAACGTGTGC 
GAACTCCATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B230118H07Rik ( XM_006500094.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500157.1 Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30834
Clone ID Related Accession (Same CDS sequence)	XM_006500094.3
Accession Version	XM_006500094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	uncharacterized protein C11orf74 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATACTTGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500157.1
CDS	472..915
Translation	MLAQIPRPEIMDEDQLMKEVLDKFVNCHEQTYEEFLSTFTHLSKEDNVAKWGAHGTDSSENIFSTVKWNHKTESNGRHFRNTSIFLHTSSEHSEEDQIVIDEGHKVGSSFQGDMTRAGKVKVDNFLDLEDVDMEEESKPQMNKDTWR

Target ORF information:

RefSeq Version	XM_006500094.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500094.3

ORF Insert Sequence:

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATACTTGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B230118H07Rik ( NM_001362688.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349617.1 Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 3, mRNA.

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CloneID	OMu84317
Clone ID Related Accession (Same CDS sequence)	NM_001362688.1
Accession Version	NM_001362688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	protein C11orf74 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929569.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CB193700.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG CTGAAGCAAG CTTTATTTAA TACTGGCCAG 
GAACATGGAC TGGCCAGGTA CCGACCTGGG ATTCCCAGAG AATGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349617.1
CDS	171..821
Translation	MLAQIPRPEIMDEDQLMKEVLDKFVNCHEQTYEEFLSTFTHLSKEDNVAKWGAHGTDSSENIFSTVKWNHKTESNGRHFRNTSIFLHTSSEHSEEDQIVIDEGHKVGSSFQGDMTRAGKVKVDNFLDLEDVDMEEESKPQMNKDCLLLPGEVEQDISTSVPSYDPSVDLPLTSEVRPKPAVGRTQKQTEELKQALFNTGQEHGLARYRPGIPREWC

Target ORF information:

RefSeq Version	NM_001362688.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA B230118H07 gene (B230118H07Rik), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362688.1

ORF Insert Sequence:

ATGCTTGCTC AAATACCGAG GCCAGAGATA ATGGATGAAG ATCAGTTAAT GAAAGAAGTC 
TTGGATAAGT TTGTCAACTG CCATGAGCAA ACCTATGAAG AATTTCTGAG CACTTTCACT 
CATCTTTCAA AAGAGGATAA TGTGGCCAAA TGGGGAGCAC ATGGAACTGA TTCATCAGAA 
AATATATTTT CTACTGTAAA ATGGAATCAT AAAACTGAAT CAAATGGTCG TCATTTTAGA 
AATACATCCA TCTTTCTTCA CACTTCATCG GAACACTCAG AAGAGGACCA GATTGTGATT 
GATGAAGGCC ACAAAGTTGG GAGTTCCTTT CAAGGTGATA TGACCCGGGC TGGAAAGGTG 
AAGGTAGACA ACTTCCTAGA CTTAGAAGAT GTGGACATGG AGGAAGAGAG TAAGCCCCAG 
ATGAACAAGG ATTGCCTGCT ACTTCCAGGT GAGGTGGAAC AGGATATAAG CACCAGCGTT 
CCATCTTATG ATCCTTCTGT GGACCTACCG CTCACCTCTG AGGTGAGGCC AAAGCCTGCT 
GTGGGAAGGA CTCAGAAACA AACCGAAGAG CTGAAGCAAG CTTTATTTAA TACTGGCCAG 
GAACATGGAC TGGCCAGGTA CCGACCTGGG ATTCCCAGAG AATGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Search for the Function of NWC, Third Gene Within RAG Locus: Generation and Characterization of NWC-Deficient Mice.
Archivum immunologiae et therapiae experimentalis64(4)311-9(2016 Aug)
Kasztura M,Sniezewski L,Laszkiewicz A,Majkowski M,Kobak K,Peczek K,Janik S,Kapusniak V,Miazek A,Cebrat M,Kisielow P

Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter.
PloS one9(9)e106927(2014)
Laszkiewicz A,Bzdzion ?,Kasztura M,Snie?ewski L,Janik S,Kisielow P,Cebrat M

Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells.
PloS one7(9)e44807(2012)
Laszkiewicz A,Sniezewski L,Kasztura M,Bzdzion L,Cebrat M,Kisielow P

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B230118H07Rik cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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