Aff4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Aff4
Entrez Gene ID	93736
Full Name	AF4/FMR2 family, member 4
Synonyms	AF5Q31,Alf4,Laf4l,MCEF
General protein information	Preferred Names AF4/FMR2 family, member 4 Names AF4/FMR2 family member 4 ALL1 fused gene from 5q31
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001363331.1	NP_001350260.1	AF4/FMR2 family member 4 isoform 2
XM_006534560.3	XP_006534623.1	AF4/FMR2 family member 4 isoform X2
XM_011249331.3	XP_011247633.1	AF4/FMR2 family member 4 isoform X1
NM_033565.2	NP_291043.1	AF4/FMR2 family member 4 isoform 1
XM_011249331.2	XP_011247633.1	AF4/FMR2 family member 4 isoform X1
XM_030246443.1	XP_030102303.1	AF4/FMR2 family member 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF4	XP_517928.2
Canis lupus familiaris (dog)	AFF4	XP_850309.1
Mus musculus (house mouse)	Aff4	NP_291043.1
Gallus gallus (chicken)	AFF4	XP_004945088.1
Danio rerio (zebrafish)	aff4	XP_684327.6
Rattus norvegicus (Norway rat)	Aff4	NP_001100471.1
Xenopus tropicalis (tropical clawed frog)	aff4	XP_002935317.2
Homo sapiens (human)	AFF4	NP_055238.1
Bos taurus (cattle)	AFF4	NP_001178058.1

Gene Ontology
Bibliography

Related articles in PubMed

Importance of a specific amino acid pairing for murine MLL leukemias driven by MLLT1/3 or AFF1/4.
Lokken AA, Achille NJ, Chang MJ, Lin JJ, Kuntimaddi A, Leach BI, Malik B, Nesbit JB, Zhang S, Bushweller JH, Zeleznik-Le NJ, Hemenway CS
Leukemia research38(11)1309-15(2014 Nov)

The RNA Pol II elongation factor Ell3 marks enhancers in ES cells and primes future gene activation.
Lin C, Garruss AS, Luo Z, Guo F, Shilatifard A
Cell152(1-2)144-56(2013 Jan)

Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.
Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y
The Journal of biological chemistry287(24)19985-96(2012 Jun)

The little elongation complex regulates small nuclear RNA transcription.
Smith ER, Lin C, Garrett AS, Thornton J, Mohaghegh N, Hu D, Jackson J, Saraf A, Swanson SK, Seidel C, Florens L, Washburn MP, Eissenberg JC, Shilatifard A
Molecular cell44(6)954-65(2011 Dec)

Differential chromatin looping regulates CD4 expression in immature thymocytes.
Jiang H, Peterlin BM
Molecular and cellular biology28(3)907-12(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

define a specific pairing of two amino acids that creates a salt bridge between MLLT1/3 and AFF proteins that is critically important for MLL-mediated transformation of HPCs
Title: Importance of a specific amino acid pairing for murine MLL leukemias driven by MLLT1/3 or AFF1/4.

AFF4 may therefore help to maintain activation of AMPK downstream signaling under conditions of prolonged stimulation with ghrelin, such as during fasting.
Title: Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.

In the absence of Runx1 on the silencer, P-TEFb interacts with the transcription complex, forming a different chromatin loop between the enhancer and the promoter, which leads to the expression of the CD4 gene
Title: Differential chromatin looping regulates CD4 expression in immature thymocytes.

Af4 functions as a positive regulator of Pol II transcription elongation factor b (P-TEFb) kinase and, in complex with MLL fusion partners Af9, Enl and Af10, as a mediator of histone H3-K79 methylation by recruiting Dot1 to elongating Pol II
Title: The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling.

AF5q31 (Aff4) is a transcriptional regulator in testicular somatic cells and is essential for male germ cell differentiation and survival.
Title: Infertility with defective spermiogenesis in mice lacking AF5q31, the target of chromosomal translocation in human infant leukemia.

The following Aff4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aff4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15411	XM_011249331.3 Latest version!	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu47156	XM_030246443.1 Latest version!	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15411	NM_033565.2 Latest version!	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu47156	NM_001363331.1 Latest version!	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu47156	XM_006534560.3 Latest version!	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu15411	XM_011249331.2	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aff4 ( XM_011249331.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247633.1 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15411
Clone ID Related Accession (Same CDS sequence)	XM_011249331.3 , NM_033565.2 , XM_011249331.2
Accession Version	XM_011249331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT 
TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC 
CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC 
AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA 
CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT 
ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG 
GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG 
AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA 
CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA 
GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA 
GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC 
TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG 
AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA 
CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA 
AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC 
AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA 
GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG 
ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT 
CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT 
AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC 
AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA 
ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA 
GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG 
AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG 
GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT 
ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA 
ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG 
ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG 
CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA 
AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA 
ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA 
AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT 
GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC 
AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA 
AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG 
AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG 
TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA 
CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA 
GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG 
ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC 
CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC 
TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT 
CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247633.1
CDS	526..4008
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_011249331.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249331.3

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT 
TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC 
CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC 
AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA 
CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT 
ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG 
GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG 
AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA 
CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA 
GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA 
GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC 
TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG 
AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA 
CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA 
AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC 
AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA 
GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG 
ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT 
CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT 
AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC 
AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA 
ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA 
GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG 
AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG 
GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT 
ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA 
ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG 
ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG 
CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA 
AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA 
ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA 
AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT 
GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC 
AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA 
AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG 
AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG 
TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA 
CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA 
GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG 
ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC 
CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC 
TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT 
CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff4 ( XM_030246443.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102303.1 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47156
Clone ID Related Accession (Same CDS sequence)	NM_001363331.1 , XM_006534560.3 , XM_030246443.1
Accession Version	XM_030246443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG 
TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC 
CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG 
GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA 
CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT 
GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT 
GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT 
GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC 
AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC 
TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA 
ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC 
AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT 
GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC 
AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA 
AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT 
AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC 
GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT 
CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT 
CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC 
ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA 
GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG 
CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC 
CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA 
TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA 
CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT 
GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC 
AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC 
AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT 
ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT 
TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG 
TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT 
GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC 
ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA 
GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG 
CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG 
AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG 
ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG 
GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG 
GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA 
GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC 
CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG 
CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102303.1
CDS	587..3982
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_030246443.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246443.1

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG 
TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC 
CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG 
GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA 
CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT 
GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT 
GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT 
GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC 
AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC 
TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA 
ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC 
AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT 
GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC 
AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA 
AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT 
AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC 
GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT 
CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT 
CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC 
ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA 
GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG 
CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC 
CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA 
TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA 
CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT 
GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC 
AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC 
AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT 
ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT 
TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG 
TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT 
GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC 
ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA 
GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG 
CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG 
AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG 
ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG 
GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG 
GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA 
GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC 
CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG 
CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff4 ( NM_033565.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291043.1 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 1, mRNA.

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CloneID	OMu15411
Clone ID Related Accession (Same CDS sequence)	XM_011249331.3 , NM_033565.2 , XM_011249331.2
Accession Version	NM_033565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ064959.1, BC138999.1 and AI536326.1. On Jul 18, 2009 this sequence version replaced NM_033565.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF190449.1, BC138999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT 
TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC 
CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC 
AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA 
CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT 
ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG 
GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG 
AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA 
CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA 
GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA 
GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC 
TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG 
AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA 
CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA 
AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC 
AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA 
GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG 
ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT 
CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT 
AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC 
AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA 
ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA 
GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG 
AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG 
GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT 
ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA 
ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG 
ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG 
CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA 
AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA 
ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA 
AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT 
GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC 
AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA 
AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG 
AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG 
TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA 
CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA 
GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG 
ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC 
CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC 
TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT 
CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291043.1
CDS	368..3850
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	NM_033565.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033565.2

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT 
TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC 
CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC 
AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA 
CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT 
ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG 
GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG 
AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA 
CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA 
GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA 
GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC 
TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG 
AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA 
CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA 
AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC 
AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA 
GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG 
ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT 
CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT 
AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC 
AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA 
ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA 
GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG 
AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG 
GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT 
ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA 
ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG 
ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG 
CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA 
AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA 
ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA 
AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT 
GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC 
AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA 
AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG 
AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG 
TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA 
CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA 
GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG 
ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC 
CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC 
TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT 
CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff4 ( NM_001363331.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350260.1 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 2, mRNA.

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CloneID	OMu47156
Clone ID Related Accession (Same CDS sequence)	NM_001363331.1 , XM_006534560.3 , XM_030246443.1
Accession Version	NM_001363331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592489.13. On May 17, 2018 this sequence version replaced XM_006534560.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG 
TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC 
CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG 
GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA 
CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT 
GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT 
GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT 
GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC 
AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC 
TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA 
ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC 
AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT 
GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC 
AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA 
AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT 
AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC 
GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT 
CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT 
CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC 
ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA 
GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG 
CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC 
CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA 
TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA 
CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT 
GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC 
AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC 
AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT 
ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT 
TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG 
TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT 
GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC 
ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA 
GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG 
CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG 
AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG 
ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG 
GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG 
GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA 
GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC 
CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG 
CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350260.1
CDS	368..3763
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	NM_001363331.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363331.1

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG 
TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC 
CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG 
GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA 
CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT 
GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT 
GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT 
GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC 
AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC 
TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA 
ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC 
AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT 
GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC 
AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA 
AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT 
AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC 
GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT 
CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT 
CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC 
ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA 
GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG 
CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC 
CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA 
TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA 
CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT 
GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC 
AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC 
AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT 
ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT 
TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG 
TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT 
GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC 
ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA 
GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG 
CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG 
AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG 
ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG 
GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG 
GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA 
GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC 
CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG 
CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff4 ( XM_011249331.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247633.1 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.

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CloneID	OMu15411
Clone ID Related Accession (Same CDS sequence)	XM_011249331.3 , NM_033565.2 , XM_011249331.2
Accession Version	XM_011249331.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT 
TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC 
CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC 
AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA 
CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT 
ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG 
GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG 
AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA 
CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA 
GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA 
GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC 
TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG 
AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA 
CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA 
AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC 
AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA 
GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG 
ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT 
CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT 
AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC 
AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA 
ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA 
GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG 
AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG 
GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT 
ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA 
ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG 
ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG 
CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA 
AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA 
ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA 
AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT 
GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC 
AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA 
AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG 
AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG 
TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA 
CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA 
GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG 
ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC 
CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC 
TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT 
CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247633.1
CDS	745..4227
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_011249331.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249331.2

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT 
TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC 
CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC 
AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA 
CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT 
ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG 
GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG 
AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA 
CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA 
GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA 
GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC 
TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG 
AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA 
CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA 
AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC 
AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA 
GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG 
ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT 
CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT 
AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC 
AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA 
ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA 
GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG 
AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG 
GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT 
ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA 
ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG 
ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG 
CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA 
AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA 
ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA 
AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT 
GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC 
AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA 
AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG 
AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG 
TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA 
CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA 
GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG 
ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC 
CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC 
TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT 
CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aff4 ( XM_006534560.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534623.1 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.

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CloneID	OMu47156
Clone ID Related Accession (Same CDS sequence)	NM_001363331.1 , XM_006534560.3 , XM_030246443.1
Accession Version	XM_006534560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG 
TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC 
CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG 
GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA 
CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT 
GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT 
GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT 
GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC 
AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC 
TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA 
ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC 
AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT 
GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC 
AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA 
AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT 
AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC 
GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT 
CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT 
CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC 
ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA 
GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG 
CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC 
CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA 
TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA 
CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT 
GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC 
AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC 
AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT 
ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT 
TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG 
TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT 
GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC 
ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA 
GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG 
CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG 
AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG 
ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG 
GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG 
GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA 
GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC 
CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG 
CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534623.1
CDS	653..4048
Translation	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRSIPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGGSGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKESQQSNFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASKPSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTRASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSGTSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_006534560.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534560.3

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA 
GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT 
GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA 
GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT 
CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG 
AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA 
AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG 
GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG 
GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC 
GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA 
TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT 
GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT 
GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG 
GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG 
TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC 
CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG 
GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA 
CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT 
GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT 
GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT 
GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC 
AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC 
TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA 
ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC 
AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT 
GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC 
AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA 
AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT 
AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC 
GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT 
CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT 
CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC 
ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA 
GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG 
CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC 
CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA 
TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA 
CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT 
GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC 
AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC 
AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT 
ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT 
TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG 
TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT 
GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC 
ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA 
GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG 
CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG 
AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG 
ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG 
GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG 
GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA 
GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC 
CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG 
CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Importance of a specific amino acid pairing for murine MLL leukemias driven by MLLT1/3 or AFF1/4.
Leukemia research38(11)1309-15(2014 Nov)
Lokken AA,Achille NJ,Chang MJ,Lin JJ,Kuntimaddi A,Leach BI,Malik B,Nesbit JB,Zhang S,Bushweller JH,Zeleznik-Le NJ,Hemenway CS

The RNA Pol II elongation factor Ell3 marks enhancers in ES cells and primes future gene activation.
Cell152(1-2)144-56(2013 Jan)
Lin C,Garruss AS,Luo Z,Guo F,Shilatifard A

Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.
The Journal of biological chemistry287(24)19985-96(2012 Jun)
Komori T,Doi A,Nosaka T,Furuta H,Akamizu T,Kitamura T,Senba E,Morikawa Y

The little elongation complex regulates small nuclear RNA transcription.
Molecular cell44(6)954-65(2011 Dec)
Smith ER,Lin C,Garrett AS,Thornton J,Mohaghegh N,Hu D,Jackson J,Saraf A,Swanson SK,Seidel C,Florens L,Washburn MP,Eissenberg JC,Shilatifard A

Differential chromatin looping regulates CD4 expression in immature thymocytes.
Molecular and cellular biology28(3)907-12(2008 Feb)
Jiang H,Peterlin BM

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Aff4 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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