NF1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

  • Gene

  • Clones

  • gRNAs

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOc06706 XM_007666669.1
Latest version!
Ornithorhynchus anatinus neurofibromin 1 (NF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OOc119297 XM_029082633.1
Latest version!
Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OOc119298 XM_029082634.1
Latest version!
Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OOc119299 XM_029082636.1
Latest version!
Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OOc119300 XM_029082637.1
Latest version!
Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOc06706
    Clone ID Related Accession (Same CDS sequence) XM_007666669.1
    Accession Version XM_007666669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8808bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-04-14
    Organism Ornithorhynchus anatinus(platypus)
    Product neurofibromin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794070.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGGCCACTT TAACGCTCCC ACCCTCAAAA CCAGAGGCGG GTATCAAGTG GGTGTTCAAT 
    CTGGAAGACC CGAAGATGCC GGGCTTGCCT GACGCTACAA GAAGAAGAAG AACATTCCCC
    CAACATTACA AGGGCAAAAT CCGACCAATG TGCATGTGCA AAAAGCACGA GACTGAATTT
    TGGGTGATAG AGTATTTCAG GAAGCTTTAT TTTGGGGGGG GCACCATCCT CTCCTGGCTG
    TACGAGATTC TTAGTTTGGT GATGGAAGGG AAAAGGCCCC CAAGAGAAGT CTGTAAGCTT
    CCAATAAAAG CCGGACAGCA GAGCACGCAC ACCAAAGTCA GTACTGAACA CAACAAGGAG
    TGTCTTATCA ACATTTCTAA ATACAAGTTT TCTTTGGTTA TAAGTGGTCT CACCAATATA
    TTAAAAAATG TTAATAATAT GAGAATATTT GGAGAAGCTG CCGAAAAGAA TTTGTATCTC
    TCTCAATTGA TTATATTGGA TACACTGGAA AAATGTCTTG CCGGGCAACC AAAGGACACC
    ATGCGATTGG ATGAGACTAT GCTGGTCAAG CAGTTGCTGC CGGAAATCTG CCATTTTATC
    CACACCTATC GGGAAGGAAA CCAACATGCA GCTGAGCTGC GGAATTCTGC CTCGGGAGTT
    CTCTTCTCTC TCAGCTGTAA TAACTTCAAT GCAGTCTTCA GCCGTATTTC CACCAGGTTA
    CAGGAGCTGA CTGTGTGCTC AGAAGATAAT GTTGATGTCC ATGATATAGA ATTGTTACAG
    TACATCAATG TGGACTGTGC AAAGCTGAAG CGACTGCTGC AGGAAACAGT ATTTAAGTTT
    AAAGCCCTTA AGAAAGTAGC TCAGCTGGCA GTTATAAACA GTCTGGAAAA AGCATTTTGG
    AACTGGGTAG AGAACTATCC TGATGAATTT ACAAAGTTGT ACCAGACTCC CCAGACAGAC
    ATGGCTGATT GTGCCGAAAA GTTGTTTGAC TTGGTGGATG GTTTTGCTGA AAGCACGAAA
    CGTAAAGCAG CAGTTTGGCC ATTGCAAATC ATTCTTCTTG TCCTGTGCCC AGAAATAATC
    CAAGATATAT CCAAGGATGT GGTGGAAGAA ACCAAAATAA ATAAGAAGTT GTTTCTGGAA
    AATCTCAGAA AAGCACTCGC TGGCCATGGT GGAAGTAGGC AACTGACTGA AAGTGCTGCT
    ATTGCTTGTG TCAAATTGTG CAAAGCAAGT ACCTACATCA ACTGGGAAGA TAACTCTGTC
    ATTTTCCTAT TAGTACAGTC CATGGTGGTA GATCTTAAGA ACCTGCTGTT TAATCCAACT
    AAACCCTTCT CAAGAGGCAA TCAGAATGCA GATGTGGATC TAATGATTGA CTGTTTAGTT
    TCTTGTTTTC GCATAAACCC TCACAACAAC CAACACTTTA AGATCTGCTT GGCCCAGAGT
    TCTCCATCCA CATTTCATTA CGTGCTGGTA AATTCACTCC ATCGAATCAT CACAAATAGC
    ACTTTCAAGC ATGGACTTGG CACTGCTTCT GCTCTGGATT GGTGGCCCAA GATTGATGCG
    GTGTACTGCC ATTCAGTTGA ACTCCGCAGC ATGTTCGGTG AAACGCTTCA CAAAGCAGTG
    CAGGGCTGTG GAGCCCATCC AGCAATACGA ATGGCCACGA GTCTTACATT CAAAGAAAAA
    ATGACAAGTC TTAAATTTAA AGAAAAACCT ACAGACTTGG AAACAAGAAG TTATAAATAT
    CTCCTGTTGT CCATGGTGAA ACTAATCCAT GCCGATCCAA AGCTGTTGCT CTGTAATCCG
    AGGAAACAAG GGCCCGAAAC CCAGGGCAGC ACAGCAGAGT TAATCACAGG ACTTGTCCAG
    CTTGTTCCCC AGTCAAACAT GCCAGAAATA GCCCAAGAAG CCATGGAGGC TTTGTTGGTT
    CTTCATCAAT TAGAGAGTAT TGATTTATGG AATCCCGATG CACCTATAGA AACATTTTGG
    GAGATTAGTT CGCAAATGCT TTTTTACATC TGTAAGAAGC TAACCAGTCA CCAGATGCTT
    AGCAGCACAG AAATTCTCAA ATGGCTGCGG GAAATTTTGA TCTGCAGGAA TAAATTCCTT
    CTTAAAAATA AGGACAGTAC AGTCGGATGC AGCGGAACTC CAATATGCCG CCAAGCTCAG
    ACTAAGCTAG AAGTTGCTCT CTACATGTTC CTCTGGAGCC CCGACATTGA GGCCGTCCTT
    GTCGCCATGT CCTGTTTCCG CCACCTCTGT GAAGAAGCTG ACATTAGGTG TGGGGTAGAT
    GAAGTTTCCG TGCATAATTT CTTGCCAAAC TACAATACCT TCATGGAGTT TGCCTCCGTC
    AGCAATATGA TGTCTACAGG AAGAGCGGCA CTTCAAAAGA GGGTGATGGC ACTGTTGAGA
    CGAATTGAAC ATCCAACTGC CGGCAACACT GAGGCATGGG AAGACACTCA TACAAAATGG
    GAACAAGCTA CGAAGCTGAT CCTTAACTAT CCAAAAGCCA AAATGGAAGA TGGCCAGGTA
    ACCGAAAGTC TTCACAAGAC CATTGTAAAG CGGCGAATGT CTCACGTGAG CGGGGGAGGA
    TCCATCGATT TGTCTGACAC GGACTCCCTG CAGGAGTGGA TCAATATGAC GGGCTTCCTT
    TGTGCTCTCG GTGGGGTGTG CCTGCAGCAC CGTGGCAACG CGGGCTTGGC CACCTACAGC
    CCACCTATGG GCCCAGTCAG TGAGCGAAAG GGTTCCATGA TTTCTATGGT ATCCACCGAA
    GGAAATACCG AGACGCCCGT CAGCAAGTTC ATGGACCGGT TGCTCTCCCT GATGGTGTGC
    AACCACGAGA AAGTGGGACT CCAAATACGG ACGAATATCA AGGACCTGGT GGGTCTCGAA
    TTAAGTCCTG CACTTTATCC GATGCTATTC AACAAGTTGA AGAACACCAT CAGCAAATTT
    TTTGACTCTC AAGGGCAGGT GTTGCTGAAT GATACCAATA CCCAATTTGT AGAGCAGACC
    ATAGCGATAA TGAAGAACTT GCTGGATAAT CACACCGAAG GCAGCTCGGA GCACCTGGGG
    CAAGCCAGCA TAGAAACAAT GATGCTCAAT CTAGTCAGGT ACGTTCGTGT GCTTGGGAAT
    TTGGTACACG CAATTCAGAT TAAAACGAAA CTGTGCCAGT TAGTTGAAGT AATGATGGAG
    AGGCGAGATG ATCTTTCGTT TTGCCAAGAA ATGAAATTTA GGAACAAGAT GGTGGAATAT
    CTGACAGATT GGGTCATGGG GACATCGAAC CAAGCAACAG ATGAAGATGT AAAGTGTTTA
    ACAAGAGATT TGGACCAGGC AAGCATGGAA GCAGTTGTGT CTCTTCTTGC TGGACTCCCC
    CTGCAGCCGG AAGAAGGAGA TGGTGTGGAA TTGATGGAAG CCAAGTCCCA GTTATTTCTT
    AAGTATTTCA CTTTGTTTAT GAACCTTCTC AATGACTGCA GTGAGGTTGA AGATGAAAAC
    GCCCAAGCGG GAGGCAGGAA ACGTGGGATG TCTCGAAGAC TGGCTTCCCT GAGGCACTGT
    ACCGTGCTTG CTATGTCAAA CTTACTCAAC GCTAACGTGG ACAGCGGCCT TATGCATTCC
    ATAGGTTTAG GTTATCACAA AGATCTCCAA ACGAGAGCCA CGTTCATGGA AGTCCTCACG
    AAAATTCTCC AGCAGGGCAC CGAGTTTGAC ACGTTAGCGG AGACCGTATT GGCCGATCGA
    TTTGAGAGGC TGGTGGAATT AGTCACGATG ATGGGTGATC AAGGAGAGCT TCCCATAGCA
    ATGGCCCTGG CCAACGTGGT ACCTTGTTCA CAATGGGATG AGTTAGCTCG GGTTCTCGTC
    ACTCTCTTTG ATTCACGCCA CTTACTCTAC CAGCTGCTTT GGAACATGTT TTCTAAGGAA
    GTTGAGCTGG CAGATTCCAT GCAGACTCTC TTCAGGGGGA ACAGTCTGGC CAGTAAAATA
    ATGACATTCT GCTTTAAGGT TTACGGTGCT ACATATCTGC AAAAGCTTTT GGAACCTTTA
    CTGAGGACTG TCATCACGTC GATGGAATGG CAGCACGTCA GCTTTGAGGT GGATCCTACA
    AGGTTAGAGC CTACAGAGAG CCTTGATGAT AACCAACGGA GTCTCTTACA AATGACTGAA
    AAGTTCTTCC ATGCTATCAT CAATTCCTCC TCAGAGTTTC CCCCTCAGCT TCGCAGTGTG
    TGCCATTGTT TGTACCAGGC AACTTGCCAC TCTCTACTGA ATAAAGCTAC AGTAAAAGAA
    AAAAAGGAAA ACAAAAAATC AGTGGTTAGC CAGCGTTTTC CTCAGAACAG TATTGGTGCA
    GTAGGAAGTG CCATGTTCCT CAGATTCATC AATCCTGCCA TTGTCTCGCC GTATGAGGCT
    GGGATCTTAG ATAAAAAGCC TCCGCCTAGA ATTGAGAGGG GCTTGAAGTT AATGTCAAAG
    ATACTTCAGA GTATTGCCAA TCATGTCCTG TTCACAAAAG AAGAGCATAT GAGGCCTTTT
    AATGACTTTG TGAAAAGCAA CTTCGATGCA GCCCGAAGGT TTTTCCTCGA TATAGCGTCT
    GATTGTCCTG CAAGCGATTC AGTAAATCAC AGCCTCTCCT TCATCAGTGA TGGCAATGTA
    CTTGCTTTAC ATCGTTTGCT CTGGAACAAC CAAGAAAAGA TCGGCCAATA TCTTTCCAGC
    AACAGGGACC ACAAGGCTGT TGGACGTCGA CCCTTTGACA AGATGGCTAC ACTCCTTGCG
    TATCTGGGCC CTCCTGAGCA CAAGCCCGTA GCAGATACCC ACTGGTCCAG CCTGAATCTG
    ACCAGTTCCA AATTTGAGGA GTTCATGACC AGACACCAGG TACATGAAAA AGAGGAATTT
    AAAGCATTGA AAACATTAAA TATCTTCTAT CAAGCAGGGA CTTCCAAGGC CGGGAATCCA
    GTTTTTTATT ACATTGCACG GAGGTTCAAG ACCGGTCAGA TCAATGGGGA TTTGCTGATA
    TACCACGTCT TGCTGACTCT CAAGCCATAT TATGCCAAGC CATATGAGAT CGTCGTGGAC
    CTTACCCACG CCGGCCCCAG CAATCGCTTT AAAACAGACT TTCTCTCCAA GTGGTTTGTC
    GTTTTCCCTG GCTTTGCGTA CGAAAATGTG TCTGCTGTTT ACATCTACAA CTGTAATTCT
    TGGGTCAGGG AGTACACCAA ATACCACGAG AGACTGCTCA CTGGCCTCAA AGGCAGCAAG
    AGACTTATTT TCATAGACTC GCCTGGAAAA TTGGCTGAGC ACATCGAGCA CGACCAGCAG
    AAACTGCCGG CTGCCACTTT GGCTTTAGAA GAGGATTTGA AGGTGTTCCA CAATGCTCTC
    AAACTGGCCC ACAAAGACAC CAAGGTTTCT ATTAAAGTTG GCTCGACTGC CGTTCAGGTC
    ACATCAGCCG AACGGACAAG GGTCTTGGGG CAGTCGGTTT TCCTGAACGA CATCTATTAC
    GCCTCAGAGA TCGAGGAGAT TTGCCTCGTT GACGAAAACC AGTTCACTTT AACCATTGCT
    AACCAAGGCA CTCCACTCAC CTTCATGCAC CAAGAGTGCG AAGCCATCGT TCAATCCATC
    ATCCACATAC GGACACGGTG GGAGCTGTCT CAACCCGACT CCATCCCCCA GCACACGAAA
    ATCCGGCCGA AGGATGTCCC CGGAACGCTC CTGAATATCG CGTTGCTTAA TCTGGGCAGC
    TCAGACCCTA GTTTACGGTC AGCTGCCTAT AATCTTCTGT GTGCCTTAAC CTGTACCTTT
    AATTTAAAGA TTGAAGGCCA GTTGCTAGAG ACTTCAGGTC TGTGTATCCC TGCCAACAAC
    ACCCTTTTTA TTGTCTCTAT TAGTAAGACC TTGGCAGCTA ATGAGCCACA CCTCACCCTG
    GAGTTTTTGG AAGAGTGTAT TTCTGGATTT AGCAAATCTA GTAATGGGTC TTGCTCTTCT
    ACCTCTTTCT CTTCAGAACA CCTACACTTA ATTTTGGAAT ATCCACATTT TAATCAAGGT
    ATTGAGCTGA AGCACCTTTG CTTAGAATAT ATGACTCCCT GGCTATCAAA TCTAGTGCGT
    TTCTGTAAAC ACAATGATGA TGCCAAACGC CAGCGAGTCA CTGCTATTCT TGACAAGCTC
    ATAACAATGA CGATTAATGA GAAACAGATG TATCCATCTA TCCAAGCCAA GATCTGGGGA
    AGTCTTGGAC AGATTACAGA TCTGCTTGAT GTCGTGCTGG ACAGTTTCAT CAAAACCAGT
    GCAACTGGTG GCTTGGGCTC TATAAAGGCT GAGGTGATGG CAGACACAGC TGTGGCTCTT
    GCTTCCGGAA ATGTGAAACT CGTTTCGAGT AAGGTCATCG GGAGAATGTG CAAAATAATC
    GACAAGACCT GCCTGTCTCC AACTCCCACC TTAGAACAAC ACCTCATGTG GGACGATATT
    GCCATCCTTG CCCGATACAT GCTGATGCTG TCCTTTAACA ACTCTCTCGA CGTGGCAGCC
    CACCTGCCCT ATCTCTTCCA TGTTGTTACT CTGTTGGTGG CCACTGGTCC TCTGTCCCTT
    AGAGCTTCCA CTCACGGACT GGTCATCAAC ATCATTCATT CTCTGTGCAC TTGTTCACAG
    CTTCATTTCA GTGAGGAGAC CAAGCAAGTT TTGAGGCTCA GCTTGACAGA GTTCTCATTA
    CCCAAGTTTT ATTTGCTGTT TGGCATCAGC AAAGTCAAGT CGGCAGCTGT CATAGCCTTC
    CGGTCCAGTT ACCGGGACAG GTCTTTCTCA CCTGGTTCCT ACGAGAGGGA GACGTTTGCC
    TTGACATCCC TGGAGACCGT GACAGAGGCT CTACTGGAGA TTATGGAGGC TTGTATGAGA
    GATATTCCAA CGTGCAAGTG GCTGGACCAA TGGACAGAAC TAGCACAAAA ATTTGCATTC
    CAGTATAATC CATCACTCCA GCCAAGAGCC CTCGTTGTCT TCGGTTGTAT CAGCAAAAGG
    GTATCTCACG GGCAGATAAA ACAAATTATC CGTATTCTTA GCAAGGGACT TGAGAGCTGT
    CTAAAGGGAC CTGATAATTA CAACAGTCAA GTTCTGATAG AAGCCACAGT AATAGCACTG
    ACCAAATTAC AGCCACTTTT GAATAAGGAT TCTCCTATGC ACAAAGCCCT CTTTTGGGTG
    GCTATGGCTG TGTTGCAGCT GGATGAAGTT AACTTGTATT CAGCTGGGAC AGCACTTCTT
    GAGCAGAACC TGCACACGTT AGATAGTCTC CGGGTATTCA ATGACAAGAG TCCAGAAGAA
    GTCTTCATGG AAATCCGAAA TCCGCTTGAA TGGCACTGCA AACAAATGGA TCATTTTGTT
    GGACTCAATT TTAACTCTAA TTTTAATTTT GCACTAGTAG GACACCTTTT AAAAGGATAC
    AGGCATCCTT CTCCTATCAT TGTTGCAAGA ACAGTAAGGA TCTTACACAC ACTACTAGCT
    CTGGTTAACA AACATAGGAA CTGTGACAAA TTTGAGGTGA ATACCCAGAG TGTGGCTTAC
    TTAGCAGCTT TGCTTACAGT GTCTGAGGAA GTGCGAAGTC GCTGTAGCCT AAAACATAGG
    AAATCACTGC TGCTGACAGA TGTCTCTATG GAAAATGTTC CTATGGATAC ATATCCCATC
    CATCACAACG ATCCCAGCTG TAGGACACTA AAGGAAAATC AACCATGGTC CTCCCCAAAG
    GGCTCCGAGA GACATCTCGC CGCCAGCTAC CCGACTGTTG GTCAGACCAG CCCCCGAACC
    CGGAAGTCCA TGAGTTTGGA TATGGGACAG CCTTCCCAGG CCAACACTAA GAAGCTGCTT
    GGAACCAGGA AAAGCTTTGA CCACTTGATA TCAGACACAA AGGCTCCTAA AAGGCAAGAA
    ATGGAATCTG GGATCACCAC CCCTCCCAAA ATGAGGAGAG TGGCCGAGAA TGACTATGAA
    ATGGAAACCC AGAGAATCTC CTCACCTCAA CAGCACCCAC ACCTGCGCAA AGTTTCTGTG
    TCTGAATCAA ATGTACTTCT TGATGAAGAA GTTCTAACCG ATCCCAAAAT CCAAGCCCTG
    CTGCTTACTG TCCTGGCTAC ACTGGTAAAA TACACTACAG ACGAGTTTGA TCAACGGATC
    CTTTATGAAT ATTTAGCCGA GGCCAGTGTT GTCTTCCCTA AAGTTTTTCC AGTTGTGCAC
    AATCTGTTGG ACTCCAAGAT TAATACCCTT CTGTCGCTAT GCCAAGATCC AAATCTACTG
    AATCCAATTC ATGGGATCGT GCAGAGCGTG GTCTACCATG AAGAGTCGCC ACCACAGTAC
    CAAACCTCTT ATCTACAAAG TTTTGGTTTT AATGGCTTGT GGAGGTTTGC GGGACCATTT
    TCTAAGCAAA CCCAAATTCC AGACTATGCT GAGCTGATTG TGAAATTTCT CGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAGCCAGTG AGGAGTCCTT ACTGACCCCC
    ACCTCTCCTT ATCCTCCAGC AGTGCAGAGC CAGCTGAGCA TTACTGCTAA TCTTAACCTT
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGCATTGA CAAGGAGAAT
    GTGGAGCTCT CTCCGACCAC TGGCCACTCC AACAGTGGGA GGACACGCCA CGGCTCCGCA
    AGCCAAGTGC AAAAGCAGCG AAGTGCCGGC AGTTTCAAAC GTCACATCTC AGACGATGTA
    TGCAGTGCAA TTTATGTAAG CGATTTTCAT GGTAAGTTGT GTTGTGGAAT TGTAGTTCGA
    ATTCGGGGAA AAAACAAACA AAACCCAGCA GGGGGTGTGT GGAGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007664859.1
    CDS1..8808
    Translation

    Target ORF information:

    RefSeq Version XM_007666669.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus neurofibromin 1 (NF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_007666669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGGCCACTT TAACGCTCCC ACCCTCAAAA CCAGAGGCGG GTATCAAGTG GGTGTTCAAT 
    CTGGAAGACC CGAAGATGCC GGGCTTGCCT GACGCTACAA GAAGAAGAAG AACATTCCCC
    CAACATTACA AGGGCAAAAT CCGACCAATG TGCATGTGCA AAAAGCACGA GACTGAATTT
    TGGGTGATAG AGTATTTCAG GAAGCTTTAT TTTGGGGGGG GCACCATCCT CTCCTGGCTG
    TACGAGATTC TTAGTTTGGT GATGGAAGGG AAAAGGCCCC CAAGAGAAGT CTGTAAGCTT
    CCAATAAAAG CCGGACAGCA GAGCACGCAC ACCAAAGTCA GTACTGAACA CAACAAGGAG
    TGTCTTATCA ACATTTCTAA ATACAAGTTT TCTTTGGTTA TAAGTGGTCT CACCAATATA
    TTAAAAAATG TTAATAATAT GAGAATATTT GGAGAAGCTG CCGAAAAGAA TTTGTATCTC
    TCTCAATTGA TTATATTGGA TACACTGGAA AAATGTCTTG CCGGGCAACC AAAGGACACC
    ATGCGATTGG ATGAGACTAT GCTGGTCAAG CAGTTGCTGC CGGAAATCTG CCATTTTATC
    CACACCTATC GGGAAGGAAA CCAACATGCA GCTGAGCTGC GGAATTCTGC CTCGGGAGTT
    CTCTTCTCTC TCAGCTGTAA TAACTTCAAT GCAGTCTTCA GCCGTATTTC CACCAGGTTA
    CAGGAGCTGA CTGTGTGCTC AGAAGATAAT GTTGATGTCC ATGATATAGA ATTGTTACAG
    TACATCAATG TGGACTGTGC AAAGCTGAAG CGACTGCTGC AGGAAACAGT ATTTAAGTTT
    AAAGCCCTTA AGAAAGTAGC TCAGCTGGCA GTTATAAACA GTCTGGAAAA AGCATTTTGG
    AACTGGGTAG AGAACTATCC TGATGAATTT ACAAAGTTGT ACCAGACTCC CCAGACAGAC
    ATGGCTGATT GTGCCGAAAA GTTGTTTGAC TTGGTGGATG GTTTTGCTGA AAGCACGAAA
    CGTAAAGCAG CAGTTTGGCC ATTGCAAATC ATTCTTCTTG TCCTGTGCCC AGAAATAATC
    CAAGATATAT CCAAGGATGT GGTGGAAGAA ACCAAAATAA ATAAGAAGTT GTTTCTGGAA
    AATCTCAGAA AAGCACTCGC TGGCCATGGT GGAAGTAGGC AACTGACTGA AAGTGCTGCT
    ATTGCTTGTG TCAAATTGTG CAAAGCAAGT ACCTACATCA ACTGGGAAGA TAACTCTGTC
    ATTTTCCTAT TAGTACAGTC CATGGTGGTA GATCTTAAGA ACCTGCTGTT TAATCCAACT
    AAACCCTTCT CAAGAGGCAA TCAGAATGCA GATGTGGATC TAATGATTGA CTGTTTAGTT
    TCTTGTTTTC GCATAAACCC TCACAACAAC CAACACTTTA AGATCTGCTT GGCCCAGAGT
    TCTCCATCCA CATTTCATTA CGTGCTGGTA AATTCACTCC ATCGAATCAT CACAAATAGC
    ACTTTCAAGC ATGGACTTGG CACTGCTTCT GCTCTGGATT GGTGGCCCAA GATTGATGCG
    GTGTACTGCC ATTCAGTTGA ACTCCGCAGC ATGTTCGGTG AAACGCTTCA CAAAGCAGTG
    CAGGGCTGTG GAGCCCATCC AGCAATACGA ATGGCCACGA GTCTTACATT CAAAGAAAAA
    ATGACAAGTC TTAAATTTAA AGAAAAACCT ACAGACTTGG AAACAAGAAG TTATAAATAT
    CTCCTGTTGT CCATGGTGAA ACTAATCCAT GCCGATCCAA AGCTGTTGCT CTGTAATCCG
    AGGAAACAAG GGCCCGAAAC CCAGGGCAGC ACAGCAGAGT TAATCACAGG ACTTGTCCAG
    CTTGTTCCCC AGTCAAACAT GCCAGAAATA GCCCAAGAAG CCATGGAGGC TTTGTTGGTT
    CTTCATCAAT TAGAGAGTAT TGATTTATGG AATCCCGATG CACCTATAGA AACATTTTGG
    GAGATTAGTT CGCAAATGCT TTTTTACATC TGTAAGAAGC TAACCAGTCA CCAGATGCTT
    AGCAGCACAG AAATTCTCAA ATGGCTGCGG GAAATTTTGA TCTGCAGGAA TAAATTCCTT
    CTTAAAAATA AGGACAGTAC AGTCGGATGC AGCGGAACTC CAATATGCCG CCAAGCTCAG
    ACTAAGCTAG AAGTTGCTCT CTACATGTTC CTCTGGAGCC CCGACATTGA GGCCGTCCTT
    GTCGCCATGT CCTGTTTCCG CCACCTCTGT GAAGAAGCTG ACATTAGGTG TGGGGTAGAT
    GAAGTTTCCG TGCATAATTT CTTGCCAAAC TACAATACCT TCATGGAGTT TGCCTCCGTC
    AGCAATATGA TGTCTACAGG AAGAGCGGCA CTTCAAAAGA GGGTGATGGC ACTGTTGAGA
    CGAATTGAAC ATCCAACTGC CGGCAACACT GAGGCATGGG AAGACACTCA TACAAAATGG
    GAACAAGCTA CGAAGCTGAT CCTTAACTAT CCAAAAGCCA AAATGGAAGA TGGCCAGGTA
    ACCGAAAGTC TTCACAAGAC CATTGTAAAG CGGCGAATGT CTCACGTGAG CGGGGGAGGA
    TCCATCGATT TGTCTGACAC GGACTCCCTG CAGGAGTGGA TCAATATGAC GGGCTTCCTT
    TGTGCTCTCG GTGGGGTGTG CCTGCAGCAC CGTGGCAACG CGGGCTTGGC CACCTACAGC
    CCACCTATGG GCCCAGTCAG TGAGCGAAAG GGTTCCATGA TTTCTATGGT ATCCACCGAA
    GGAAATACCG AGACGCCCGT CAGCAAGTTC ATGGACCGGT TGCTCTCCCT GATGGTGTGC
    AACCACGAGA AAGTGGGACT CCAAATACGG ACGAATATCA AGGACCTGGT GGGTCTCGAA
    TTAAGTCCTG CACTTTATCC GATGCTATTC AACAAGTTGA AGAACACCAT CAGCAAATTT
    TTTGACTCTC AAGGGCAGGT GTTGCTGAAT GATACCAATA CCCAATTTGT AGAGCAGACC
    ATAGCGATAA TGAAGAACTT GCTGGATAAT CACACCGAAG GCAGCTCGGA GCACCTGGGG
    CAAGCCAGCA TAGAAACAAT GATGCTCAAT CTAGTCAGGT ACGTTCGTGT GCTTGGGAAT
    TTGGTACACG CAATTCAGAT TAAAACGAAA CTGTGCCAGT TAGTTGAAGT AATGATGGAG
    AGGCGAGATG ATCTTTCGTT TTGCCAAGAA ATGAAATTTA GGAACAAGAT GGTGGAATAT
    CTGACAGATT GGGTCATGGG GACATCGAAC CAAGCAACAG ATGAAGATGT AAAGTGTTTA
    ACAAGAGATT TGGACCAGGC AAGCATGGAA GCAGTTGTGT CTCTTCTTGC TGGACTCCCC
    CTGCAGCCGG AAGAAGGAGA TGGTGTGGAA TTGATGGAAG CCAAGTCCCA GTTATTTCTT
    AAGTATTTCA CTTTGTTTAT GAACCTTCTC AATGACTGCA GTGAGGTTGA AGATGAAAAC
    GCCCAAGCGG GAGGCAGGAA ACGTGGGATG TCTCGAAGAC TGGCTTCCCT GAGGCACTGT
    ACCGTGCTTG CTATGTCAAA CTTACTCAAC GCTAACGTGG ACAGCGGCCT TATGCATTCC
    ATAGGTTTAG GTTATCACAA AGATCTCCAA ACGAGAGCCA CGTTCATGGA AGTCCTCACG
    AAAATTCTCC AGCAGGGCAC CGAGTTTGAC ACGTTAGCGG AGACCGTATT GGCCGATCGA
    TTTGAGAGGC TGGTGGAATT AGTCACGATG ATGGGTGATC AAGGAGAGCT TCCCATAGCA
    ATGGCCCTGG CCAACGTGGT ACCTTGTTCA CAATGGGATG AGTTAGCTCG GGTTCTCGTC
    ACTCTCTTTG ATTCACGCCA CTTACTCTAC CAGCTGCTTT GGAACATGTT TTCTAAGGAA
    GTTGAGCTGG CAGATTCCAT GCAGACTCTC TTCAGGGGGA ACAGTCTGGC CAGTAAAATA
    ATGACATTCT GCTTTAAGGT TTACGGTGCT ACATATCTGC AAAAGCTTTT GGAACCTTTA
    CTGAGGACTG TCATCACGTC GATGGAATGG CAGCACGTCA GCTTTGAGGT GGATCCTACA
    AGGTTAGAGC CTACAGAGAG CCTTGATGAT AACCAACGGA GTCTCTTACA AATGACTGAA
    AAGTTCTTCC ATGCTATCAT CAATTCCTCC TCAGAGTTTC CCCCTCAGCT TCGCAGTGTG
    TGCCATTGTT TGTACCAGGC AACTTGCCAC TCTCTACTGA ATAAAGCTAC AGTAAAAGAA
    AAAAAGGAAA ACAAAAAATC AGTGGTTAGC CAGCGTTTTC CTCAGAACAG TATTGGTGCA
    GTAGGAAGTG CCATGTTCCT CAGATTCATC AATCCTGCCA TTGTCTCGCC GTATGAGGCT
    GGGATCTTAG ATAAAAAGCC TCCGCCTAGA ATTGAGAGGG GCTTGAAGTT AATGTCAAAG
    ATACTTCAGA GTATTGCCAA TCATGTCCTG TTCACAAAAG AAGAGCATAT GAGGCCTTTT
    AATGACTTTG TGAAAAGCAA CTTCGATGCA GCCCGAAGGT TTTTCCTCGA TATAGCGTCT
    GATTGTCCTG CAAGCGATTC AGTAAATCAC AGCCTCTCCT TCATCAGTGA TGGCAATGTA
    CTTGCTTTAC ATCGTTTGCT CTGGAACAAC CAAGAAAAGA TCGGCCAATA TCTTTCCAGC
    AACAGGGACC ACAAGGCTGT TGGACGTCGA CCCTTTGACA AGATGGCTAC ACTCCTTGCG
    TATCTGGGCC CTCCTGAGCA CAAGCCCGTA GCAGATACCC ACTGGTCCAG CCTGAATCTG
    ACCAGTTCCA AATTTGAGGA GTTCATGACC AGACACCAGG TACATGAAAA AGAGGAATTT
    AAAGCATTGA AAACATTAAA TATCTTCTAT CAAGCAGGGA CTTCCAAGGC CGGGAATCCA
    GTTTTTTATT ACATTGCACG GAGGTTCAAG ACCGGTCAGA TCAATGGGGA TTTGCTGATA
    TACCACGTCT TGCTGACTCT CAAGCCATAT TATGCCAAGC CATATGAGAT CGTCGTGGAC
    CTTACCCACG CCGGCCCCAG CAATCGCTTT AAAACAGACT TTCTCTCCAA GTGGTTTGTC
    GTTTTCCCTG GCTTTGCGTA CGAAAATGTG TCTGCTGTTT ACATCTACAA CTGTAATTCT
    TGGGTCAGGG AGTACACCAA ATACCACGAG AGACTGCTCA CTGGCCTCAA AGGCAGCAAG
    AGACTTATTT TCATAGACTC GCCTGGAAAA TTGGCTGAGC ACATCGAGCA CGACCAGCAG
    AAACTGCCGG CTGCCACTTT GGCTTTAGAA GAGGATTTGA AGGTGTTCCA CAATGCTCTC
    AAACTGGCCC ACAAAGACAC CAAGGTTTCT ATTAAAGTTG GCTCGACTGC CGTTCAGGTC
    ACATCAGCCG AACGGACAAG GGTCTTGGGG CAGTCGGTTT TCCTGAACGA CATCTATTAC
    GCCTCAGAGA TCGAGGAGAT TTGCCTCGTT GACGAAAACC AGTTCACTTT AACCATTGCT
    AACCAAGGCA CTCCACTCAC CTTCATGCAC CAAGAGTGCG AAGCCATCGT TCAATCCATC
    ATCCACATAC GGACACGGTG GGAGCTGTCT CAACCCGACT CCATCCCCCA GCACACGAAA
    ATCCGGCCGA AGGATGTCCC CGGAACGCTC CTGAATATCG CGTTGCTTAA TCTGGGCAGC
    TCAGACCCTA GTTTACGGTC AGCTGCCTAT AATCTTCTGT GTGCCTTAAC CTGTACCTTT
    AATTTAAAGA TTGAAGGCCA GTTGCTAGAG ACTTCAGGTC TGTGTATCCC TGCCAACAAC
    ACCCTTTTTA TTGTCTCTAT TAGTAAGACC TTGGCAGCTA ATGAGCCACA CCTCACCCTG
    GAGTTTTTGG AAGAGTGTAT TTCTGGATTT AGCAAATCTA GTAATGGGTC TTGCTCTTCT
    ACCTCTTTCT CTTCAGAACA CCTACACTTA ATTTTGGAAT ATCCACATTT TAATCAAGGT
    ATTGAGCTGA AGCACCTTTG CTTAGAATAT ATGACTCCCT GGCTATCAAA TCTAGTGCGT
    TTCTGTAAAC ACAATGATGA TGCCAAACGC CAGCGAGTCA CTGCTATTCT TGACAAGCTC
    ATAACAATGA CGATTAATGA GAAACAGATG TATCCATCTA TCCAAGCCAA GATCTGGGGA
    AGTCTTGGAC AGATTACAGA TCTGCTTGAT GTCGTGCTGG ACAGTTTCAT CAAAACCAGT
    GCAACTGGTG GCTTGGGCTC TATAAAGGCT GAGGTGATGG CAGACACAGC TGTGGCTCTT
    GCTTCCGGAA ATGTGAAACT CGTTTCGAGT AAGGTCATCG GGAGAATGTG CAAAATAATC
    GACAAGACCT GCCTGTCTCC AACTCCCACC TTAGAACAAC ACCTCATGTG GGACGATATT
    GCCATCCTTG CCCGATACAT GCTGATGCTG TCCTTTAACA ACTCTCTCGA CGTGGCAGCC
    CACCTGCCCT ATCTCTTCCA TGTTGTTACT CTGTTGGTGG CCACTGGTCC TCTGTCCCTT
    AGAGCTTCCA CTCACGGACT GGTCATCAAC ATCATTCATT CTCTGTGCAC TTGTTCACAG
    CTTCATTTCA GTGAGGAGAC CAAGCAAGTT TTGAGGCTCA GCTTGACAGA GTTCTCATTA
    CCCAAGTTTT ATTTGCTGTT TGGCATCAGC AAAGTCAAGT CGGCAGCTGT CATAGCCTTC
    CGGTCCAGTT ACCGGGACAG GTCTTTCTCA CCTGGTTCCT ACGAGAGGGA GACGTTTGCC
    TTGACATCCC TGGAGACCGT GACAGAGGCT CTACTGGAGA TTATGGAGGC TTGTATGAGA
    GATATTCCAA CGTGCAAGTG GCTGGACCAA TGGACAGAAC TAGCACAAAA ATTTGCATTC
    CAGTATAATC CATCACTCCA GCCAAGAGCC CTCGTTGTCT TCGGTTGTAT CAGCAAAAGG
    GTATCTCACG GGCAGATAAA ACAAATTATC CGTATTCTTA GCAAGGGACT TGAGAGCTGT
    CTAAAGGGAC CTGATAATTA CAACAGTCAA GTTCTGATAG AAGCCACAGT AATAGCACTG
    ACCAAATTAC AGCCACTTTT GAATAAGGAT TCTCCTATGC ACAAAGCCCT CTTTTGGGTG
    GCTATGGCTG TGTTGCAGCT GGATGAAGTT AACTTGTATT CAGCTGGGAC AGCACTTCTT
    GAGCAGAACC TGCACACGTT AGATAGTCTC CGGGTATTCA ATGACAAGAG TCCAGAAGAA
    GTCTTCATGG AAATCCGAAA TCCGCTTGAA TGGCACTGCA AACAAATGGA TCATTTTGTT
    GGACTCAATT TTAACTCTAA TTTTAATTTT GCACTAGTAG GACACCTTTT AAAAGGATAC
    AGGCATCCTT CTCCTATCAT TGTTGCAAGA ACAGTAAGGA TCTTACACAC ACTACTAGCT
    CTGGTTAACA AACATAGGAA CTGTGACAAA TTTGAGGTGA ATACCCAGAG TGTGGCTTAC
    TTAGCAGCTT TGCTTACAGT GTCTGAGGAA GTGCGAAGTC GCTGTAGCCT AAAACATAGG
    AAATCACTGC TGCTGACAGA TGTCTCTATG GAAAATGTTC CTATGGATAC ATATCCCATC
    CATCACAACG ATCCCAGCTG TAGGACACTA AAGGAAAATC AACCATGGTC CTCCCCAAAG
    GGCTCCGAGA GACATCTCGC CGCCAGCTAC CCGACTGTTG GTCAGACCAG CCCCCGAACC
    CGGAAGTCCA TGAGTTTGGA TATGGGACAG CCTTCCCAGG CCAACACTAA GAAGCTGCTT
    GGAACCAGGA AAAGCTTTGA CCACTTGATA TCAGACACAA AGGCTCCTAA AAGGCAAGAA
    ATGGAATCTG GGATCACCAC CCCTCCCAAA ATGAGGAGAG TGGCCGAGAA TGACTATGAA
    ATGGAAACCC AGAGAATCTC CTCACCTCAA CAGCACCCAC ACCTGCGCAA AGTTTCTGTG
    TCTGAATCAA ATGTACTTCT TGATGAAGAA GTTCTAACCG ATCCCAAAAT CCAAGCCCTG
    CTGCTTACTG TCCTGGCTAC ACTGGTAAAA TACACTACAG ACGAGTTTGA TCAACGGATC
    CTTTATGAAT ATTTAGCCGA GGCCAGTGTT GTCTTCCCTA AAGTTTTTCC AGTTGTGCAC
    AATCTGTTGG ACTCCAAGAT TAATACCCTT CTGTCGCTAT GCCAAGATCC AAATCTACTG
    AATCCAATTC ATGGGATCGT GCAGAGCGTG GTCTACCATG AAGAGTCGCC ACCACAGTAC
    CAAACCTCTT ATCTACAAAG TTTTGGTTTT AATGGCTTGT GGAGGTTTGC GGGACCATTT
    TCTAAGCAAA CCCAAATTCC AGACTATGCT GAGCTGATTG TGAAATTTCT CGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAGCCAGTG AGGAGTCCTT ACTGACCCCC
    ACCTCTCCTT ATCCTCCAGC AGTGCAGAGC CAGCTGAGCA TTACTGCTAA TCTTAACCTT
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGCATTGA CAAGGAGAAT
    GTGGAGCTCT CTCCGACCAC TGGCCACTCC AACAGTGGGA GGACACGCCA CGGCTCCGCA
    AGCCAAGTGC AAAAGCAGCG AAGTGCCGGC AGTTTCAAAC GTCACATCTC AGACGATGTA
    TGCAGTGCAA TTTATGTAAG CGATTTTCAT GGTAAGTTGT GTTGTGGAAT TGTAGTTCGA
    ATTCGGGGAA AAAACAAACA AAACCCAGCA GGGGGTGTGT GGAGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc119297
    Clone ID Related Accession (Same CDS sequence) XM_029082633.1
    Accession Version XM_029082633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041744.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGGAGTCC AAAAACGTAG GTTCTGCCCC TCACTACGCC GCTTGTCTGC TGTGGGACCT 
    TGGCTTCCAA TAAAAGCCGG ACAGCAGAGC ACGCACACCA AAGTCAGTAC TGAACACAAC
    AAGGAGTGTC TTATCAACAT TTCTAAATAC AAGTTTTCTT TGGTTATAAG TGGTCTCACC
    AATATATTAA AAAATGTTAA TAATATGAGA ATATTTGGAG AAGCTGCCGA AAAGAATTTG
    TATCTCTCTC AATTGATTAT ATTGGATACA CTGGAAAAAT GTCTTGCCGG GCAACCAAAG
    GACACCATGC GATTGGATGA GACTATGCTG GTCAAGCAGT TGCTGCCGGA AATCTGCCAT
    TTTATCCACA CCTATCGGGA AGGAAACCAA CATGCAGCTG AGCTGCGGAA TTCTGCCTCG
    GGAGTTCTCT TCTCTCTCAG CTGTAATAAC TTCAATGCAG TCTTCAGCCG TATTTCCACC
    AGGTTACAGG AGCTGACTGT GTGCTCAGAA GATAATGTTG ATGTCCATGA TATAGAATTG
    TTACAGTACA TCAATGTGGA CTGTGCAAAG CTGAAGCGAC TGCTGCAGGA AACAGTATTT
    AAGTTTAAAG CCCTTAAGAA AGTAGCTCAG CTGGCAGTTA TAAACAGTCT GGAAAAAGCA
    TTTTGGAACT GGGTAGAGAA CTATCCTGAT GAATTTACAA AGTTGTACCA GACTCCCCAG
    ACAGACATGG CTGATTGTGC CGAAAAGTTG TTTGACTTGG TGGATGGTTT TGCTGAAAGC
    ACGAAACGTA AAGCAGCAGT TTGGCCATTG CAAATCATTC TTCTTGTCCT GTGCCCAGAA
    ATAATCCAAG ATATATCCAA GGATGTGGTG GAAGAAACCA AAATAAATAA GAAGTTGTTT
    CTGGAAAATC TCAGAAAAGC ACTCGCTGGC CATGGTGGAA GTAGGCAACT GACTGAAAGT
    GCTGCTATTG CTTGTGTCAA ATTGTGCAAA GCAAGTACCT ACATCAACTG GGAAGATAAC
    TCTGTCATTT TCCTATTAGT ACAGTCCATG GTGGTAGATC TTAAGAACCT GCTGTTTAAT
    CCAACTAAAC CCTTCTCAAG AGGCAATCAG AATGCAGATG TGGATCTAAT GATTGACTGT
    TTAGTTTCTT GTTTTCGCAT AAACCCTCAC AACAACCAAC ACTTTAAGAT CTGCTTGGCC
    CAGAGTTCTC CATCCACATT TCATTACGTG CTGGTAAATT CACTCCATCG AATCATCACA
    AATTCTGCTC TGGATTGGTG GCCCAAGATT GATGCGGTGT ACTGCCATTC AGTTGAACTC
    CGCAGCATGT TCGGTGAAAC GCTTCACAAA GCAGTGCAGG GCTGTGGAGC CCATCCAGCA
    ATACGAATGG CCACGAGTCT TACATTCAAA GAAAAAATGA CAAGTCTTAA ATTTAAAGAA
    AAACCTACAG ACTTGGAAAC AAGAAGTTAT AAATATCTCC TGTTGTCCAT GGTGAAACTA
    ATCCATGCCG ATCCAAAGCT GTTGCTCTGT AATCCGAGGA AACAAGGGCC CGAAACCCAG
    GGCAGCACAG CAGAGTTAAT CACAGGACTT GTCCAGCTTG TTCCCCAGTC AAACATGCCA
    GAAATAGCCC AAGAAGCCAT GGAGGCTTTG TTGGTTCTTC ATCAATTAGA GAGTATTGAT
    TTATGGAATC CTGATGCACC TATAGAAACA TTTTGGGAGA TTAGTTCGCA AATGCTTTTT
    TACATCTGTA AGAAGCTAAC CAGTCACCAG ATGCTTAGCA GCACAGAAAT TCTCAAATGG
    CTGCGGGAAA TTTTGATCTG CAGGAATAAA TTCCTTCTTA AAAATAAGCA GTCAGATAGG
    AGTTCCTGCC ACTTCCTTTT CCTTTATGGG GTAGCATGTG ACGTCTCTTC TGGTGGGGGT
    GCTAGTCAAA TCTCTCTAGA CCATGAAGAG TTAATACGCA CCTGTACTCC GGGAGCATCC
    CTCAGGAAGG GGAAAGGAAA CTCATCCATG GACAGTACAG TCGGATGCAG CGGAACTCCA
    ATATGCCGCC AAGCTCAGAC TAAGCTAGAA GTTGCTCTCT ACATGTTCCT CTGGAGCCCC
    GACATTGAGG CCGTCCTTGT CGCCATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCTGAC
    ATTAGGTGTG GGGTAGATGA AGTTTCCGTG CATAATTTCT TGCCAAACTA CAATACCTTC
    ATGGAGTTTG CCTCCGTCAG CAATATGATG TCTACAGGAA GAGCGGCACT TCAAAAGAGG
    GTGATGGCAC TGTTGAGACG AATTGAACAT CCAACTGCCG GCAACACTGA GGCATGGGAA
    GACACTCATA CAAAATGGGA ACAAGCTACG AAGCTGATCC TTAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGTAAC CGAAAGTCTT CACAAGACCA TTGTAAAGCG GCGAATGTCT
    CACGTGAGCG GGGGAGGATC CATCGATTTG TCTGACACGG ACTCCCTGCA GGAGTGGATC
    AATATGACGG GCTTCCTTTG TGCTCTCGGT GGGGTGTGCC TGCAGCACCG TGGCAACGCG
    GGCTTGGCCA CCTACAGCCC ACCTATGGGC CCAGTCAGTG AGCGAAAGGG TTCCATGATT
    TCTATGGTAT CCACCGAAGG AAATACCGAG ACGCCCGTCA GCAAGTTCAT GGACCGGTTG
    CTCTCCCTGA TGGTGTGCAA CCACGAGAAA GTGGGACTCC AAATACGGAC GAATATCAAG
    GACCTGGTGG GTCTCGAATT AAGTCCTGCA CTTTATCCGA TGCTATTCAA CAAGTTGAAG
    AACACCATCA GCAAATTTTT TGACTCTCAA GGGCAGGTGT TGCTGAATGA TACCAATACC
    CAATTTGTAG AGCAGACCAT AGCGATAATG AAGAACTTGC TGGATAATCA CACCGAAGGC
    AGCTCGGAGC ACCTGGGGCA AGCCAGCATA GAAACAATGA TGCTCAATCT AGTCAGGTAC
    GTTCGTGTGC TTGGGAATTT GGTACACGCA ATTCAGATTA AAACGAAACT GTGCCAGTTA
    GTTGAAGTAA TGATGGAGAG GCGAGATGAT CTTTCGTTTT GCCAAGAAAT GAAATTTAGG
    AACAAGATGG TGGAATATCT GACAGATTGG GTCATGGGGA CATCGAACCA AGCAACAGAT
    GAAGATGTAA AGTGTTTAAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTTGTGTCT
    CTTCTTGCTG GACTCCCCCT GCAGCCGGAA GAAGGAGATG GTGTGGAATT GATGGAAGCC
    AAGTCCCAGT TATTTCTTAA GTATTTCACT TTGTTTATGA ACCTTCTCAA TGACTGCAGT
    GAGGTTGAAG ATGAAAACGC CCAAGCGGGA GGCAGGAAAC GTGGGATGTC TCGAAGACTG
    GCTTCCCTGA GGCACTGTAC CGTGCTTGCT ATGTCAAACT TACTCAACGC TAACGTGGAC
    AGCGGCCTTA TGCATTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAAAC GAGAGCCACG
    TTCATGGAAG TCCTCACGAA AATTCTCCAG CAGGGCACCG AGTTTGACAC GTTAGCGGAG
    ACCGTATTGG CCGATCGATT TGAGAGGCTG GTGGAATTAG TCACGATGAT GGGTGATCAA
    GGAGAGCTTC CCATAGCAAT GGCCCTGGCC AACGTGGTAC CTTGTTCACA ATGGGATGAG
    TTAGCTCGGG TTCTCGTCAC TCTCTTTGAT TCACGCCACT TACTCTACCA GCTGCTTTGG
    AACATGTTTT CTAAGGAAGT TGAGCTGGCA GATTCCATGC AGACTCTCTT CAGGGGGAAC
    AGTCTGGCCA GTAAAATAAT GACATTCTGC TTTAAGGTTT ACGGTGCTAC ATATCTGCAA
    AAGCTTTTGG AACCTTTACT GAGGACTGTC ATCACGTCGA TGGAATGGCA GCACGTCAGC
    TTTGAGGTGG ATCCTACAAG GTTAGAGCCT ACAGAGAGCC TTGATGATAA CCAACGGAGT
    CTCTTACAAA TGACTGAAAA GTTCTTCCAT GCTATCATCA ATTCCTCCTC AGAGTTTCCC
    CCTCAGCTTC GCAGTGTGTG CCATTGTTTG TACCAGGCAA CTTGCCACTC TCTACTGAAT
    AAAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGTTTTCCT
    CAGAACAGTA TTGGTGCAGT AGGAAGTGCC ATGTTCCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAGGCTGG GATCTTAGAT AAAAAGCCTC CGCCTAGAAT TGAGAGGGGC
    TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT ATTGCCAATC ATGTCCTGTT CACAAAAGAA
    GAGCATATGA GGCCTTTTAA TGACTTTGTG AAAAGCAACT TCGATGCAGC CCGAAGGTTT
    TTCCTCGATA TAGCGTCTGA TTGTCCTGCA AGCGATTCAG TAAATCACAG CCTCTCCTTC
    ATCAGTGATG GCAATGTACT TGCTTTACAT CGTTTGCTCT GGAACAACCA AGAAAAGATC
    GGCCAATATC TTTCCAGCAA CAGGGACCAC AAGGCTGTTG GACGTCGACC CTTTGACAAG
    ATGGCTACAC TCCTTGCGTA TCTGGGCCCT CCTGAGCACA AGCCCGTAGC AGATACCCAC
    TGGTCCAGCC TGAATCTGAC CAGTTCCAAA TTTGAGGAGT TCATGACCAG ACACCAGGTA
    CATGAAAAAG AGGAATTTAA AGCATTGAAA ACATTAAATA TCTTCTATCA AGCAGGGACT
    TCCAAGGCCG GGAATCCAGT TTTTTATTAC ATTGCACGGA GGTTCAAGAC CGGTCAGATC
    AATGGGGATT TGCTGATATA CCACGTCTTG CTGACTCTCA AGCCATATTA TGCCAAGCCA
    TATGAGATCG TCGTGGACCT TACCCACGCC GGCCCCAGCA ATCGCTTTAA AACAGACTTT
    CTCTCCAAGT GGTTTGTCGT TTTCCCTGGC TTTGCGTACG AAAATGTGTC TGCTGTTTAC
    ATCTACAACT GTAATTCTTG GGTCAGGGAG TACACCAAAT ACCACGAGAG ACTGCTCACT
    GGCCTCAAAG GCAGCAAGAG ACTTATTTTC ATAGACTCGC CTGGAAAATT GGCTGAGCAC
    ATCGAGCACG ACCAGCAGAA ACTGCCGGCT GCCACTTTGG CTTTAGAAGA GGATTTGAAG
    GTGTTCCACA ATGCTCTCAA ACTGGCCCAC AAAGACACCA AGGTTTCTAT TAAAGTTGGC
    TCGACTGCCG TTCAGGTCAC ATCAGCCGAA CGGACAAGGG TCTTGGGGCA GTCGGTTTTC
    CTGAACGACA TCTATTACGC CTCAGAGATC GAGGAGATCT GCCTCGTTGA CGAAAACCAG
    TTCACTTTAA CCATTGCTAA CCAAGGCACT CCACTCACCT TCATGCACCA AGAGTGCGAA
    GCCATCGTTC AATCCATCAT CCACATACGG ACACGGTGGG AGCTGTCTCA ACCCGACTCC
    ATCCCCCAGC ACACGAAAAT CCGGCCGAAG GATGTCCCCG GAACGCTCCT GAATATCGCG
    TTGCTTAATC TGGGCAGCTC AGACCCTAGT TTACGGTCAG CTGCCTATAA TCTTCTGTGT
    GCCTTAACCT GTACCTTTAA TTTAAAGATT GAAGGCCAGT TGCTAGAGAC TTCAGGTCTG
    TGTATCCCTG CCAACAACAC CCTTTTTATT GTCTCTATTA GTAAGACCTT GGCAGCTAAT
    GAGCCACACC TCACCCTGGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAGCTGA AGCACCTTTG CTTAGAATAT ATGACTCCCT GGCTATCAAA TCTAGTGCGT
    TTCTGTAAAC ACAATGATGA TGCCAAACGC CAGCGAGTCA CTGCTATTCT TGACAAGCTC
    ATAACAATGA CGATTAATGA GAAACAGATG TATCCATCTA TCCAAGCCAA GATCTGGGGA
    AGTCTTGGAC AGATTACAGA TCTGCTTGAT GTCGTGCTGG ACAGTTTCAT CAAAACCAGT
    GCAACTGGTG GCTTGGGCTC TATAAAGGCT GAGGTGATGG CAGACACAGC TGTGGCTCTT
    GCTTCCGGAA ATGTGAAACT CGTTTCGAGT AAGGTCATCG GGAGAATGTG CAAAATAATC
    GACAAGACCT GCCTGTCTCC AACTCCCACC TTAGAACAAC ACCTCATGTG GGACGATATT
    GCCATCCTTG CCCGATACAT GCTGATGCTG TCCTTTAACA ACTCTCTCGA CGTGGCAGCC
    CACCTGCCCT ATCTCTTCCA TGTTGTTACT CTGTTGGTGG CCACTGGTCC TCTGTCCCTT
    AGAGCTTCCA CTCACGGACT GGTCATCAAC ATCATTCATT CTCTGTGCAC TTGTTCACAG
    CTTCATTTCA GTGAGGAGAC CAAGCAAGTT TTGAGGCTCA GCTTGACAGA GTTCTCATTA
    CCCAAGTTTT ATTTGCTGTT TGGCATCAGC AAAGTCAAGT CGGCAGCTGT CATAGCCTTC
    CGGTCCAGTT ACCGGGACAG GTCTTTCTCA CCTGGTTCCT ACGAGAGGGA GACGTTTGCC
    TTGACATCCC TGGAGACCGT GACAGAGGCT CTACTGGAGA TTATGGAGGC TTGTATGAGA
    GATATTCCAA CGTGCAAGTG GCTGGACCAA TGGACAGAAC TAGCACAAAA ATTTGCATTC
    CAGTATAATC CATCACTCCA GCCAAGAGCC CTCGTTGTCT TCGGTTGTAT CAGCAAAAGG
    GTATCTCACG GGCAGATAAA ACAAATTATC CGTATTCTTA GCAAGGGACT TGAGAGCTGT
    CTAAAGGGAC CTGATAATTA CAACAGTCAA GTTCTGATAG AAGCCACAGT AATAGCACTG
    ACCAAATTAC AGCCACTTTT GAATAAGGAT TCTCCTATGC ACAAAGCCCT CTTTTGGGTG
    GCTATGGCTG TGTTGCAGCT GGATGAAGTT AACTTGTATT CAGCTGGGAC AGCACTTCTT
    GAGCAGAACC TGCACACGTT AGATAGTCTC CGGGTATTCA ATGACAAGAG TCCAGAAGAA
    GTCTTCATGG AAATCCGAAA TCCGCTTGAA TGGCACTGCA AACAAATGGA TCATTTTGTT
    GGACTCAATT TTAACTCTAA TTTTAATTTT GCACTAGTAG GACACCTTTT AAAAGGATAC
    AGGCATCCTT CTCCTATCAT TGTTGCAAGA ACAGTAAGGA TCTTACACAC ACTACTAGCT
    CTGGTTAACA AACATAGGAA CTGTGACAAA TTTGAGGTGA ATACCCAGAG TGTGGCTTAC
    TTAGCAGCTT TGCTTACAGT GTCTGAGGAA GTGCGAAGTC GCTGTAGCCT AAAACATAGG
    AAATCACTGC TGCTGACAGA TGTCTCTATG GAAAATGTTC CTATGGATAC ATATCCCATC
    CATCACAACG ATCCCAGCTG TAGGACACTA AAGGAAAATC AACCATGGTC CTCCCCAAAG
    GGCTCCGAGA GACATCTCGC CGCCAGCTAC CCGACTGTTG GTCAGACCAG CCCCCGAACC
    CGGAAGTCCA TGAGTTTGGA TATGGGACAG CCTTCCCAGG CCAACACTAA GAAGCTGCTT
    GGAACCAGGA AAAGCTTTGA CCACTTGATA TCAGACACAA AGGCTCCTAA AAGGCAAGAA
    ATGGAATCTG GGATCACCAC CCCTCCCAAA ATGAGGAGAG TGGCCGAGAA TGACTATGAA
    ATGGAAACCC AGAGAATCTC CTCACCTCAA CAGCACCCAC ACCTGCGCAA AGTTTCTGTG
    TCTGAATCAA ATGTACTTCT TGATGAAGAA GTTCTAACCG ATCCCAAAAT CCAAGCCCTG
    CTGCTTACTG TCCTGGCTAC ACTGGTAAAA TACACTACAG ACGAGTTTGA TCAACGGATC
    CTTTATGAAT ATTTAGCCGA GGCCAGTGTT GTCTTCCCTA AAGTTTTTCC AGTTGTGCAC
    AATCTGTTGG ACTCCAAGAT TAATACCCTT CTGTCGCTAT GCCAAGATCC AAATCTACTG
    AATCCAATTC ATGGGATCGT GCAGAGCGTG GTCTACCATG AAGAGTCGCC ACCACAGTAC
    CAAACCTCTT ATCTACAAAG TTTTGGTTTT AATGGCTTGT GGAGGTTTGC GGGACCATTT
    TCTAAGCAAA CCCAAATTCC AGACTATGCT GAGCTGATTG TGAAATTTCT CGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAGCCAGTG AGGAGTCCTT ACTGACCCCC
    ACCTCTCCTT ATCCTCCAGC AGTGCAGAGC CAGCTGAGCA TTACTGCTAA TCTTAACCTT
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGCATTGA CAAGGAGAAT
    GTGGAGCTCT CTCCGACCAC TGGCCACTCC AACAGTGGGA GGACACGCCA CGGCTCCGCA
    AGCCAAGTGC AAAAGCAGCG AAGTGCCGGC AGTTTCAAAC GTCACAGTAT CAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028938466.1
    CDS16..8541
    Translation

    Target ORF information:

    RefSeq Version XM_029082633.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029082633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGGAGTCC AAAAACGTAG GTTCTGCCCC TCACTACGCC GCTTGTCTGC TGTGGGACCT 
    TGGCTTCCAA TAAAAGCCGG ACAGCAGAGC ACGCACACCA AAGTCAGTAC TGAACACAAC
    AAGGAGTGTC TTATCAACAT TTCTAAATAC AAGTTTTCTT TGGTTATAAG TGGTCTCACC
    AATATATTAA AAAATGTTAA TAATATGAGA ATATTTGGAG AAGCTGCCGA AAAGAATTTG
    TATCTCTCTC AATTGATTAT ATTGGATACA CTGGAAAAAT GTCTTGCCGG GCAACCAAAG
    GACACCATGC GATTGGATGA GACTATGCTG GTCAAGCAGT TGCTGCCGGA AATCTGCCAT
    TTTATCCACA CCTATCGGGA AGGAAACCAA CATGCAGCTG AGCTGCGGAA TTCTGCCTCG
    GGAGTTCTCT TCTCTCTCAG CTGTAATAAC TTCAATGCAG TCTTCAGCCG TATTTCCACC
    AGGTTACAGG AGCTGACTGT GTGCTCAGAA GATAATGTTG ATGTCCATGA TATAGAATTG
    TTACAGTACA TCAATGTGGA CTGTGCAAAG CTGAAGCGAC TGCTGCAGGA AACAGTATTT
    AAGTTTAAAG CCCTTAAGAA AGTAGCTCAG CTGGCAGTTA TAAACAGTCT GGAAAAAGCA
    TTTTGGAACT GGGTAGAGAA CTATCCTGAT GAATTTACAA AGTTGTACCA GACTCCCCAG
    ACAGACATGG CTGATTGTGC CGAAAAGTTG TTTGACTTGG TGGATGGTTT TGCTGAAAGC
    ACGAAACGTA AAGCAGCAGT TTGGCCATTG CAAATCATTC TTCTTGTCCT GTGCCCAGAA
    ATAATCCAAG ATATATCCAA GGATGTGGTG GAAGAAACCA AAATAAATAA GAAGTTGTTT
    CTGGAAAATC TCAGAAAAGC ACTCGCTGGC CATGGTGGAA GTAGGCAACT GACTGAAAGT
    GCTGCTATTG CTTGTGTCAA ATTGTGCAAA GCAAGTACCT ACATCAACTG GGAAGATAAC
    TCTGTCATTT TCCTATTAGT ACAGTCCATG GTGGTAGATC TTAAGAACCT GCTGTTTAAT
    CCAACTAAAC CCTTCTCAAG AGGCAATCAG AATGCAGATG TGGATCTAAT GATTGACTGT
    TTAGTTTCTT GTTTTCGCAT AAACCCTCAC AACAACCAAC ACTTTAAGAT CTGCTTGGCC
    CAGAGTTCTC CATCCACATT TCATTACGTG CTGGTAAATT CACTCCATCG AATCATCACA
    AATTCTGCTC TGGATTGGTG GCCCAAGATT GATGCGGTGT ACTGCCATTC AGTTGAACTC
    CGCAGCATGT TCGGTGAAAC GCTTCACAAA GCAGTGCAGG GCTGTGGAGC CCATCCAGCA
    ATACGAATGG CCACGAGTCT TACATTCAAA GAAAAAATGA CAAGTCTTAA ATTTAAAGAA
    AAACCTACAG ACTTGGAAAC AAGAAGTTAT AAATATCTCC TGTTGTCCAT GGTGAAACTA
    ATCCATGCCG ATCCAAAGCT GTTGCTCTGT AATCCGAGGA AACAAGGGCC CGAAACCCAG
    GGCAGCACAG CAGAGTTAAT CACAGGACTT GTCCAGCTTG TTCCCCAGTC AAACATGCCA
    GAAATAGCCC AAGAAGCCAT GGAGGCTTTG TTGGTTCTTC ATCAATTAGA GAGTATTGAT
    TTATGGAATC CTGATGCACC TATAGAAACA TTTTGGGAGA TTAGTTCGCA AATGCTTTTT
    TACATCTGTA AGAAGCTAAC CAGTCACCAG ATGCTTAGCA GCACAGAAAT TCTCAAATGG
    CTGCGGGAAA TTTTGATCTG CAGGAATAAA TTCCTTCTTA AAAATAAGCA GTCAGATAGG
    AGTTCCTGCC ACTTCCTTTT CCTTTATGGG GTAGCATGTG ACGTCTCTTC TGGTGGGGGT
    GCTAGTCAAA TCTCTCTAGA CCATGAAGAG TTAATACGCA CCTGTACTCC GGGAGCATCC
    CTCAGGAAGG GGAAAGGAAA CTCATCCATG GACAGTACAG TCGGATGCAG CGGAACTCCA
    ATATGCCGCC AAGCTCAGAC TAAGCTAGAA GTTGCTCTCT ACATGTTCCT CTGGAGCCCC
    GACATTGAGG CCGTCCTTGT CGCCATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCTGAC
    ATTAGGTGTG GGGTAGATGA AGTTTCCGTG CATAATTTCT TGCCAAACTA CAATACCTTC
    ATGGAGTTTG CCTCCGTCAG CAATATGATG TCTACAGGAA GAGCGGCACT TCAAAAGAGG
    GTGATGGCAC TGTTGAGACG AATTGAACAT CCAACTGCCG GCAACACTGA GGCATGGGAA
    GACACTCATA CAAAATGGGA ACAAGCTACG AAGCTGATCC TTAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGTAAC CGAAAGTCTT CACAAGACCA TTGTAAAGCG GCGAATGTCT
    CACGTGAGCG GGGGAGGATC CATCGATTTG TCTGACACGG ACTCCCTGCA GGAGTGGATC
    AATATGACGG GCTTCCTTTG TGCTCTCGGT GGGGTGTGCC TGCAGCACCG TGGCAACGCG
    GGCTTGGCCA CCTACAGCCC ACCTATGGGC CCAGTCAGTG AGCGAAAGGG TTCCATGATT
    TCTATGGTAT CCACCGAAGG AAATACCGAG ACGCCCGTCA GCAAGTTCAT GGACCGGTTG
    CTCTCCCTGA TGGTGTGCAA CCACGAGAAA GTGGGACTCC AAATACGGAC GAATATCAAG
    GACCTGGTGG GTCTCGAATT AAGTCCTGCA CTTTATCCGA TGCTATTCAA CAAGTTGAAG
    AACACCATCA GCAAATTTTT TGACTCTCAA GGGCAGGTGT TGCTGAATGA TACCAATACC
    CAATTTGTAG AGCAGACCAT AGCGATAATG AAGAACTTGC TGGATAATCA CACCGAAGGC
    AGCTCGGAGC ACCTGGGGCA AGCCAGCATA GAAACAATGA TGCTCAATCT AGTCAGGTAC
    GTTCGTGTGC TTGGGAATTT GGTACACGCA ATTCAGATTA AAACGAAACT GTGCCAGTTA
    GTTGAAGTAA TGATGGAGAG GCGAGATGAT CTTTCGTTTT GCCAAGAAAT GAAATTTAGG
    AACAAGATGG TGGAATATCT GACAGATTGG GTCATGGGGA CATCGAACCA AGCAACAGAT
    GAAGATGTAA AGTGTTTAAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTTGTGTCT
    CTTCTTGCTG GACTCCCCCT GCAGCCGGAA GAAGGAGATG GTGTGGAATT GATGGAAGCC
    AAGTCCCAGT TATTTCTTAA GTATTTCACT TTGTTTATGA ACCTTCTCAA TGACTGCAGT
    GAGGTTGAAG ATGAAAACGC CCAAGCGGGA GGCAGGAAAC GTGGGATGTC TCGAAGACTG
    GCTTCCCTGA GGCACTGTAC CGTGCTTGCT ATGTCAAACT TACTCAACGC TAACGTGGAC
    AGCGGCCTTA TGCATTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAAAC GAGAGCCACG
    TTCATGGAAG TCCTCACGAA AATTCTCCAG CAGGGCACCG AGTTTGACAC GTTAGCGGAG
    ACCGTATTGG CCGATCGATT TGAGAGGCTG GTGGAATTAG TCACGATGAT GGGTGATCAA
    GGAGAGCTTC CCATAGCAAT GGCCCTGGCC AACGTGGTAC CTTGTTCACA ATGGGATGAG
    TTAGCTCGGG TTCTCGTCAC TCTCTTTGAT TCACGCCACT TACTCTACCA GCTGCTTTGG
    AACATGTTTT CTAAGGAAGT TGAGCTGGCA GATTCCATGC AGACTCTCTT CAGGGGGAAC
    AGTCTGGCCA GTAAAATAAT GACATTCTGC TTTAAGGTTT ACGGTGCTAC ATATCTGCAA
    AAGCTTTTGG AACCTTTACT GAGGACTGTC ATCACGTCGA TGGAATGGCA GCACGTCAGC
    TTTGAGGTGG ATCCTACAAG GTTAGAGCCT ACAGAGAGCC TTGATGATAA CCAACGGAGT
    CTCTTACAAA TGACTGAAAA GTTCTTCCAT GCTATCATCA ATTCCTCCTC AGAGTTTCCC
    CCTCAGCTTC GCAGTGTGTG CCATTGTTTG TACCAGGCAA CTTGCCACTC TCTACTGAAT
    AAAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGTTTTCCT
    CAGAACAGTA TTGGTGCAGT AGGAAGTGCC ATGTTCCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAGGCTGG GATCTTAGAT AAAAAGCCTC CGCCTAGAAT TGAGAGGGGC
    TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT ATTGCCAATC ATGTCCTGTT CACAAAAGAA
    GAGCATATGA GGCCTTTTAA TGACTTTGTG AAAAGCAACT TCGATGCAGC CCGAAGGTTT
    TTCCTCGATA TAGCGTCTGA TTGTCCTGCA AGCGATTCAG TAAATCACAG CCTCTCCTTC
    ATCAGTGATG GCAATGTACT TGCTTTACAT CGTTTGCTCT GGAACAACCA AGAAAAGATC
    GGCCAATATC TTTCCAGCAA CAGGGACCAC AAGGCTGTTG GACGTCGACC CTTTGACAAG
    ATGGCTACAC TCCTTGCGTA TCTGGGCCCT CCTGAGCACA AGCCCGTAGC AGATACCCAC
    TGGTCCAGCC TGAATCTGAC CAGTTCCAAA TTTGAGGAGT TCATGACCAG ACACCAGGTA
    CATGAAAAAG AGGAATTTAA AGCATTGAAA ACATTAAATA TCTTCTATCA AGCAGGGACT
    TCCAAGGCCG GGAATCCAGT TTTTTATTAC ATTGCACGGA GGTTCAAGAC CGGTCAGATC
    AATGGGGATT TGCTGATATA CCACGTCTTG CTGACTCTCA AGCCATATTA TGCCAAGCCA
    TATGAGATCG TCGTGGACCT TACCCACGCC GGCCCCAGCA ATCGCTTTAA AACAGACTTT
    CTCTCCAAGT GGTTTGTCGT TTTCCCTGGC TTTGCGTACG AAAATGTGTC TGCTGTTTAC
    ATCTACAACT GTAATTCTTG GGTCAGGGAG TACACCAAAT ACCACGAGAG ACTGCTCACT
    GGCCTCAAAG GCAGCAAGAG ACTTATTTTC ATAGACTCGC CTGGAAAATT GGCTGAGCAC
    ATCGAGCACG ACCAGCAGAA ACTGCCGGCT GCCACTTTGG CTTTAGAAGA GGATTTGAAG
    GTGTTCCACA ATGCTCTCAA ACTGGCCCAC AAAGACACCA AGGTTTCTAT TAAAGTTGGC
    TCGACTGCCG TTCAGGTCAC ATCAGCCGAA CGGACAAGGG TCTTGGGGCA GTCGGTTTTC
    CTGAACGACA TCTATTACGC CTCAGAGATC GAGGAGATCT GCCTCGTTGA CGAAAACCAG
    TTCACTTTAA CCATTGCTAA CCAAGGCACT CCACTCACCT TCATGCACCA AGAGTGCGAA
    GCCATCGTTC AATCCATCAT CCACATACGG ACACGGTGGG AGCTGTCTCA ACCCGACTCC
    ATCCCCCAGC ACACGAAAAT CCGGCCGAAG GATGTCCCCG GAACGCTCCT GAATATCGCG
    TTGCTTAATC TGGGCAGCTC AGACCCTAGT TTACGGTCAG CTGCCTATAA TCTTCTGTGT
    GCCTTAACCT GTACCTTTAA TTTAAAGATT GAAGGCCAGT TGCTAGAGAC TTCAGGTCTG
    TGTATCCCTG CCAACAACAC CCTTTTTATT GTCTCTATTA GTAAGACCTT GGCAGCTAAT
    GAGCCACACC TCACCCTGGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAGCTGA AGCACCTTTG CTTAGAATAT ATGACTCCCT GGCTATCAAA TCTAGTGCGT
    TTCTGTAAAC ACAATGATGA TGCCAAACGC CAGCGAGTCA CTGCTATTCT TGACAAGCTC
    ATAACAATGA CGATTAATGA GAAACAGATG TATCCATCTA TCCAAGCCAA GATCTGGGGA
    AGTCTTGGAC AGATTACAGA TCTGCTTGAT GTCGTGCTGG ACAGTTTCAT CAAAACCAGT
    GCAACTGGTG GCTTGGGCTC TATAAAGGCT GAGGTGATGG CAGACACAGC TGTGGCTCTT
    GCTTCCGGAA ATGTGAAACT CGTTTCGAGT AAGGTCATCG GGAGAATGTG CAAAATAATC
    GACAAGACCT GCCTGTCTCC AACTCCCACC TTAGAACAAC ACCTCATGTG GGACGATATT
    GCCATCCTTG CCCGATACAT GCTGATGCTG TCCTTTAACA ACTCTCTCGA CGTGGCAGCC
    CACCTGCCCT ATCTCTTCCA TGTTGTTACT CTGTTGGTGG CCACTGGTCC TCTGTCCCTT
    AGAGCTTCCA CTCACGGACT GGTCATCAAC ATCATTCATT CTCTGTGCAC TTGTTCACAG
    CTTCATTTCA GTGAGGAGAC CAAGCAAGTT TTGAGGCTCA GCTTGACAGA GTTCTCATTA
    CCCAAGTTTT ATTTGCTGTT TGGCATCAGC AAAGTCAAGT CGGCAGCTGT CATAGCCTTC
    CGGTCCAGTT ACCGGGACAG GTCTTTCTCA CCTGGTTCCT ACGAGAGGGA GACGTTTGCC
    TTGACATCCC TGGAGACCGT GACAGAGGCT CTACTGGAGA TTATGGAGGC TTGTATGAGA
    GATATTCCAA CGTGCAAGTG GCTGGACCAA TGGACAGAAC TAGCACAAAA ATTTGCATTC
    CAGTATAATC CATCACTCCA GCCAAGAGCC CTCGTTGTCT TCGGTTGTAT CAGCAAAAGG
    GTATCTCACG GGCAGATAAA ACAAATTATC CGTATTCTTA GCAAGGGACT TGAGAGCTGT
    CTAAAGGGAC CTGATAATTA CAACAGTCAA GTTCTGATAG AAGCCACAGT AATAGCACTG
    ACCAAATTAC AGCCACTTTT GAATAAGGAT TCTCCTATGC ACAAAGCCCT CTTTTGGGTG
    GCTATGGCTG TGTTGCAGCT GGATGAAGTT AACTTGTATT CAGCTGGGAC AGCACTTCTT
    GAGCAGAACC TGCACACGTT AGATAGTCTC CGGGTATTCA ATGACAAGAG TCCAGAAGAA
    GTCTTCATGG AAATCCGAAA TCCGCTTGAA TGGCACTGCA AACAAATGGA TCATTTTGTT
    GGACTCAATT TTAACTCTAA TTTTAATTTT GCACTAGTAG GACACCTTTT AAAAGGATAC
    AGGCATCCTT CTCCTATCAT TGTTGCAAGA ACAGTAAGGA TCTTACACAC ACTACTAGCT
    CTGGTTAACA AACATAGGAA CTGTGACAAA TTTGAGGTGA ATACCCAGAG TGTGGCTTAC
    TTAGCAGCTT TGCTTACAGT GTCTGAGGAA GTGCGAAGTC GCTGTAGCCT AAAACATAGG
    AAATCACTGC TGCTGACAGA TGTCTCTATG GAAAATGTTC CTATGGATAC ATATCCCATC
    CATCACAACG ATCCCAGCTG TAGGACACTA AAGGAAAATC AACCATGGTC CTCCCCAAAG
    GGCTCCGAGA GACATCTCGC CGCCAGCTAC CCGACTGTTG GTCAGACCAG CCCCCGAACC
    CGGAAGTCCA TGAGTTTGGA TATGGGACAG CCTTCCCAGG CCAACACTAA GAAGCTGCTT
    GGAACCAGGA AAAGCTTTGA CCACTTGATA TCAGACACAA AGGCTCCTAA AAGGCAAGAA
    ATGGAATCTG GGATCACCAC CCCTCCCAAA ATGAGGAGAG TGGCCGAGAA TGACTATGAA
    ATGGAAACCC AGAGAATCTC CTCACCTCAA CAGCACCCAC ACCTGCGCAA AGTTTCTGTG
    TCTGAATCAA ATGTACTTCT TGATGAAGAA GTTCTAACCG ATCCCAAAAT CCAAGCCCTG
    CTGCTTACTG TCCTGGCTAC ACTGGTAAAA TACACTACAG ACGAGTTTGA TCAACGGATC
    CTTTATGAAT ATTTAGCCGA GGCCAGTGTT GTCTTCCCTA AAGTTTTTCC AGTTGTGCAC
    AATCTGTTGG ACTCCAAGAT TAATACCCTT CTGTCGCTAT GCCAAGATCC AAATCTACTG
    AATCCAATTC ATGGGATCGT GCAGAGCGTG GTCTACCATG AAGAGTCGCC ACCACAGTAC
    CAAACCTCTT ATCTACAAAG TTTTGGTTTT AATGGCTTGT GGAGGTTTGC GGGACCATTT
    TCTAAGCAAA CCCAAATTCC AGACTATGCT GAGCTGATTG TGAAATTTCT CGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAGCCAGTG AGGAGTCCTT ACTGACCCCC
    ACCTCTCCTT ATCCTCCAGC AGTGCAGAGC CAGCTGAGCA TTACTGCTAA TCTTAACCTT
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGCATTGA CAAGGAGAAT
    GTGGAGCTCT CTCCGACCAC TGGCCACTCC AACAGTGGGA GGACACGCCA CGGCTCCGCA
    AGCCAAGTGC AAAAGCAGCG AAGTGCCGGC AGTTTCAAAC GTCACAGTAT CAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc119298
    Clone ID Related Accession (Same CDS sequence) XM_029082634.1
    Accession Version XM_029082634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041744.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAAGCCGGT GGAGTGGGTC CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AAGCCGGACA GCAGAGCACG CACACCAAAG TCAGTACTGA ACACAACAAG
    GAGTGTCTTA TCAACATTTC TAAATACAAG TTTTCTTTGG TTATAAGTGG TCTCACCAAT
    ATATTAAAAA ATGTTAATAA TATGAGAATA TTTGGAGAAG CTGCCGAAAA GAATTTGTAT
    CTCTCTCAAT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC
    ACCATGCGAT TGGATGAGAC TATGCTGGTC AAGCAGTTGC TGCCGGAAAT CTGCCATTTT
    ATCCACACCT ATCGGGAAGG AAACCAACAT GCAGCTGAGC TGCGGAATTC TGCCTCGGGA
    GTTCTCTTCT CTCTCAGCTG TAATAACTTC AATGCAGTCT TCAGCCGTAT TTCCACCAGG
    TTACAGGAGC TGACTGTGTG CTCAGAAGAT AATGTTGATG TCCATGATAT AGAATTGTTA
    CAGTACATCA ATGTGGACTG TGCAAAGCTG AAGCGACTGC TGCAGGAAAC AGTATTTAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGCTG GCAGTTATAA ACAGTCTGGA AAAAGCATTT
    TGGAACTGGG TAGAGAACTA TCCTGATGAA TTTACAAAGT TGTACCAGAC TCCCCAGACA
    GACATGGCTG ATTGTGCCGA AAAGTTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACG
    AAACGTAAAG CAGCAGTTTG GCCATTGCAA ATCATTCTTC TTGTCCTGTG CCCAGAAATA
    ATCCAAGATA TATCCAAGGA TGTGGTGGAA GAAACCAAAA TAAATAAGAA GTTGTTTCTG
    GAAAATCTCA GAAAAGCACT CGCTGGCCAT GGTGGAAGTA GGCAACTGAC TGAAAGTGCT
    GCTATTGCTT GTGTCAAATT GTGCAAAGCA AGTACCTACA TCAACTGGGA AGATAACTCT
    GTCATTTTCC TATTAGTACA GTCCATGGTG GTAGATCTTA AGAACCTGCT GTTTAATCCA
    ACTAAACCCT TCTCAAGAGG CAATCAGAAT GCAGATGTGG ATCTAATGAT TGACTGTTTA
    GTTTCTTGTT TTCGCATAAA CCCTCACAAC AACCAACACT TTAAGATCTG CTTGGCCCAG
    AGTTCTCCAT CCACATTTCA TTACGTGCTG GTAAATTCAC TCCATCGAAT CATCACAAAT
    TCTGCTCTGG ATTGGTGGCC CAAGATTGAT GCGGTGTACT GCCATTCAGT TGAACTCCGC
    AGCATGTTCG GTGAAACGCT TCACAAAGCA GTGCAGGGCT GTGGAGCCCA TCCAGCAATA
    CGAATGGCCA CGAGTCTTAC ATTCAAAGAA AAAATGACAA GTCTTAAATT TAAAGAAAAA
    CCTACAGACT TGGAAACAAG AAGTTATAAA TATCTCCTGT TGTCCATGGT GAAACTAATC
    CATGCCGATC CAAAGCTGTT GCTCTGTAAT CCGAGGAAAC AAGGGCCCGA AACCCAGGGC
    AGCACAGCAG AGTTAATCAC AGGACTTGTC CAGCTTGTTC CCCAGTCAAA CATGCCAGAA
    ATAGCCCAAG AAGCCATGGA GGCTTTGTTG GTTCTTCATC AATTAGAGAG TATTGATTTA
    TGGAATCCTG ATGCACCTAT AGAAACATTT TGGGAGATTA GTTCGCAAAT GCTTTTTTAC
    ATCTGTAAGA AGCTAACCAG TCACCAGATG CTTAGCAGCA CAGAAATTCT CAAATGGCTG
    CGGGAAATTT TGATCTGCAG GAATAAATTC CTTCTTAAAA ATAAGCAGTC AGATAGGAGT
    TCCTGCCACT TCCTTTTCCT TTATGGGGTA GCATGTGACG TCTCTTCTGG TGGGGGTGCT
    AGTCAAATCT CTCTAGACCA TGAAGAGTTA ATACGCACCT GTACTCCGGG AGCATCCCTC
    AGGAAGGGGA AAGGAAACTC ATCCATGGAC AGTACAGTCG GATGCAGCGG AACTCCAATA
    TGCCGCCAAG CTCAGACTAA GCTAGAAGTT GCTCTCTACA TGTTCCTCTG GAGCCCCGAC
    ATTGAGGCCG TCCTTGTCGC CATGTCCTGT TTCCGCCACC TCTGTGAAGA AGCTGACATT
    AGGTGTGGGG TAGATGAAGT TTCCGTGCAT AATTTCTTGC CAAACTACAA TACCTTCATG
    GAGTTTGCCT CCGTCAGCAA TATGATGTCT ACAGGAAGAG CGGCACTTCA AAAGAGGGTG
    ATGGCACTGT TGAGACGAAT TGAACATCCA ACTGCCGGCA ACACTGAGGC ATGGGAAGAC
    ACTCATACAA AATGGGAACA AGCTACGAAG CTGATCCTTA ACTATCCAAA AGCCAAAATG
    GAAGATGGCC AGGTAACCGA AAGTCTTCAC AAGACCATTG TAAAGCGGCG AATGTCTCAC
    GTGAGCGGGG GAGGATCCAT CGATTTGTCT GACACGGACT CCCTGCAGGA GTGGATCAAT
    ATGACGGGCT TCCTTTGTGC TCTCGGTGGG GTGTGCCTGC AGCACCGTGG CAACGCGGGC
    TTGGCCACCT ACAGCCCACC TATGGGCCCA GTCAGTGAGC GAAAGGGTTC CATGATTTCT
    ATGGTATCCA CCGAAGGAAA TACCGAGACG CCCGTCAGCA AGTTCATGGA CCGGTTGCTC
    TCCCTGATGG TGTGCAACCA CGAGAAAGTG GGACTCCAAA TACGGACGAA TATCAAGGAC
    CTGGTGGGTC TCGAATTAAG TCCTGCACTT TATCCGATGC TATTCAACAA GTTGAAGAAC
    ACCATCAGCA AATTTTTTGA CTCTCAAGGG CAGGTGTTGC TGAATGATAC CAATACCCAA
    TTTGTAGAGC AGACCATAGC GATAATGAAG AACTTGCTGG ATAATCACAC CGAAGGCAGC
    TCGGAGCACC TGGGGCAAGC CAGCATAGAA ACAATGATGC TCAATCTAGT CAGGTACGTT
    CGTGTGCTTG GGAATTTGGT ACACGCAATT CAGATTAAAA CGAAACTGTG CCAGTTAGTT
    GAAGTAATGA TGGAGAGGCG AGATGATCTT TCGTTTTGCC AAGAAATGAA ATTTAGGAAC
    AAGATGGTGG AATATCTGAC AGATTGGGTC ATGGGGACAT CGAACCAAGC AACAGATGAA
    GATGTAAAGT GTTTAACAAG AGATTTGGAC CAGGCAAGCA TGGAAGCAGT TGTGTCTCTT
    CTTGCTGGAC TCCCCCTGCA GCCGGAAGAA GGAGATGGTG TGGAATTGAT GGAAGCCAAG
    TCCCAGTTAT TTCTTAAGTA TTTCACTTTG TTTATGAACC TTCTCAATGA CTGCAGTGAG
    GTTGAAGATG AAAACGCCCA AGCGGGAGGC AGGAAACGTG GGATGTCTCG AAGACTGGCT
    TCCCTGAGGC ACTGTACCGT GCTTGCTATG TCAAACTTAC TCAACGCTAA CGTGGACAGC
    GGCCTTATGC ATTCCATAGG TTTAGGTTAT CACAAAGATC TCCAAACGAG AGCCACGTTC
    ATGGAAGTCC TCACGAAAAT TCTCCAGCAG GGCACCGAGT TTGACACGTT AGCGGAGACC
    GTATTGGCCG ATCGATTTGA GAGGCTGGTG GAATTAGTCA CGATGATGGG TGATCAAGGA
    GAGCTTCCCA TAGCAATGGC CCTGGCCAAC GTGGTACCTT GTTCACAATG GGATGAGTTA
    GCTCGGGTTC TCGTCACTCT CTTTGATTCA CGCCACTTAC TCTACCAGCT GCTTTGGAAC
    ATGTTTTCTA AGGAAGTTGA GCTGGCAGAT TCCATGCAGA CTCTCTTCAG GGGGAACAGT
    CTGGCCAGTA AAATAATGAC ATTCTGCTTT AAGGTTTACG GTGCTACATA TCTGCAAAAG
    CTTTTGGAAC CTTTACTGAG GACTGTCATC ACGTCGATGG AATGGCAGCA CGTCAGCTTT
    GAGGTGGATC CTACAAGGTT AGAGCCTACA GAGAGCCTTG ATGATAACCA ACGGAGTCTC
    TTACAAATGA CTGAAAAGTT CTTCCATGCT ATCATCAATT CCTCCTCAGA GTTTCCCCCT
    CAGCTTCGCA GTGTGTGCCA TTGTTTGTAC CAGGCAACTT GCCACTCTCT ACTGAATAAA
    GCTACAGTAA AAGAAAAAAA GGAAAACAAA AAATCAGTGG TTAGCCAGCG TTTTCCTCAG
    AACAGTATTG GTGCAGTAGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AGGCTGGGAT CTTAGATAAA AAGCCTCCGC CTAGAATTGA GAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TCCTGTTCAC AAAAGAAGAG
    CATATGAGGC CTTTTAATGA CTTTGTGAAA AGCAACTTCG ATGCAGCCCG AAGGTTTTTC
    CTCGATATAG CGTCTGATTG TCCTGCAAGC GATTCAGTAA ATCACAGCCT CTCCTTCATC
    AGTGATGGCA ATGTACTTGC TTTACATCGT TTGCTCTGGA ACAACCAAGA AAAGATCGGC
    CAATATCTTT CCAGCAACAG GGACCACAAG GCTGTTGGAC GTCGACCCTT TGACAAGATG
    GCTACACTCC TTGCGTATCT GGGCCCTCCT GAGCACAAGC CCGTAGCAGA TACCCACTGG
    TCCAGCCTGA ATCTGACCAG TTCCAAATTT GAGGAGTTCA TGACCAGACA CCAGGTACAT
    GAAAAAGAGG AATTTAAAGC ATTGAAAACA TTAAATATCT TCTATCAAGC AGGGACTTCC
    AAGGCCGGGA ATCCAGTTTT TTATTACATT GCACGGAGGT TCAAGACCGG TCAGATCAAT
    GGGGATTTGC TGATATACCA CGTCTTGCTG ACTCTCAAGC CATATTATGC CAAGCCATAT
    GAGATCGTCG TGGACCTTAC CCACGCCGGC CCCAGCAATC GCTTTAAAAC AGACTTTCTC
    TCCAAGTGGT TTGTCGTTTT CCCTGGCTTT GCGTACGAAA ATGTGTCTGC TGTTTACATC
    TACAACTGTA ATTCTTGGGT CAGGGAGTAC ACCAAATACC ACGAGAGACT GCTCACTGGC
    CTCAAAGGCA GCAAGAGACT TATTTTCATA GACTCGCCTG GAAAATTGGC TGAGCACATC
    GAGCACGACC AGCAGAAACT GCCGGCTGCC ACTTTGGCTT TAGAAGAGGA TTTGAAGGTG
    TTCCACAATG CTCTCAAACT GGCCCACAAA GACACCAAGG TTTCTATTAA AGTTGGCTCG
    ACTGCCGTTC AGGTCACATC AGCCGAACGG ACAAGGGTCT TGGGGCAGTC GGTTTTCCTG
    AACGACATCT ATTACGCCTC AGAGATCGAG GAGATCTGCC TCGTTGACGA AAACCAGTTC
    ACTTTAACCA TTGCTAACCA AGGCACTCCA CTCACCTTCA TGCACCAAGA GTGCGAAGCC
    ATCGTTCAAT CCATCATCCA CATACGGACA CGGTGGGAGC TGTCTCAACC CGACTCCATC
    CCCCAGCACA CGAAAATCCG GCCGAAGGAT GTCCCCGGAA CGCTCCTGAA TATCGCGTTG
    CTTAATCTGG GCAGCTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACCTGTA CCTTTAATTT AAAGATTGAA GGCCAGTTGC TAGAGACTTC AGGTCTGTGT
    ATCCCTGCCA ACAACACCCT TTTTATTGTC TCTATTAGTA AGACCTTGGC AGCTAATGAG
    CCACACCTCA CCCTGGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAGCTGAAGC ACCTTTGCTT AGAATATATG ACTCCCTGGC TATCAAATCT AGTGCGTTTC
    TGTAAACACA ATGATGATGC CAAACGCCAG CGAGTCACTG CTATTCTTGA CAAGCTCATA
    ACAATGACGA TTAATGAGAA ACAGATGTAT CCATCTATCC AAGCCAAGAT CTGGGGAAGT
    CTTGGACAGA TTACAGATCT GCTTGATGTC GTGCTGGACA GTTTCATCAA AACCAGTGCA
    ACTGGTGGCT TGGGCTCTAT AAAGGCTGAG GTGATGGCAG ACACAGCTGT GGCTCTTGCT
    TCCGGAAATG TGAAACTCGT TTCGAGTAAG GTCATCGGGA GAATGTGCAA AATAATCGAC
    AAGACCTGCC TGTCTCCAAC TCCCACCTTA GAACAACACC TCATGTGGGA CGATATTGCC
    ATCCTTGCCC GATACATGCT GATGCTGTCC TTTAACAACT CTCTCGACGT GGCAGCCCAC
    CTGCCCTATC TCTTCCATGT TGTTACTCTG TTGGTGGCCA CTGGTCCTCT GTCCCTTAGA
    GCTTCCACTC ACGGACTGGT CATCAACATC ATTCATTCTC TGTGCACTTG TTCACAGCTT
    CATTTCAGTG AGGAGACCAA GCAAGTTTTG AGGCTCAGCT TGACAGAGTT CTCATTACCC
    AAGTTTTATT TGCTGTTTGG CATCAGCAAA GTCAAGTCGG CAGCTGTCAT AGCCTTCCGG
    TCCAGTTACC GGGACAGGTC TTTCTCACCT GGTTCCTACG AGAGGGAGAC GTTTGCCTTG
    ACATCCCTGG AGACCGTGAC AGAGGCTCTA CTGGAGATTA TGGAGGCTTG TATGAGAGAT
    ATTCCAACGT GCAAGTGGCT GGACCAATGG ACAGAACTAG CACAAAAATT TGCATTCCAG
    TATAATCCAT CACTCCAGCC AAGAGCCCTC GTTGTCTTCG GTTGTATCAG CAAAAGGGTA
    TCTCACGGGC AGATAAAACA AATTATCCGT ATTCTTAGCA AGGGACTTGA GAGCTGTCTA
    AAGGGACCTG ATAATTACAA CAGTCAAGTT CTGATAGAAG CCACAGTAAT AGCACTGACC
    AAATTACAGC CACTTTTGAA TAAGGATTCT CCTATGCACA AAGCCCTCTT TTGGGTGGCT
    ATGGCTGTGT TGCAGCTGGA TGAAGTTAAC TTGTATTCAG CTGGGACAGC ACTTCTTGAG
    CAGAACCTGC ACACGTTAGA TAGTCTCCGG GTATTCAATG ACAAGAGTCC AGAAGAAGTC
    TTCATGGAAA TCCGAAATCC GCTTGAATGG CACTGCAAAC AAATGGATCA TTTTGTTGGA
    CTCAATTTTA ACTCTAATTT TAATTTTGCA CTAGTAGGAC ACCTTTTAAA AGGATACAGG
    CATCCTTCTC CTATCATTGT TGCAAGAACA GTAAGGATCT TACACACACT ACTAGCTCTG
    GTTAACAAAC ATAGGAACTG TGACAAATTT GAGGTGAATA CCCAGAGTGT GGCTTACTTA
    GCAGCTTTGC TTACAGTGTC TGAGGAAGTG CGAAGTCGCT GTAGCCTAAA ACATAGGAAA
    TCACTGCTGC TGACAGATGT CTCTATGGAA AATGTTCCTA TGGATACATA TCCCATCCAT
    CACAACGATC CCAGCTGTAG GACACTAAAG GAAAATCAAC CATGGTCCTC CCCAAAGGGC
    TCCGAGAGAC ATCTCGCCGC CAGCTACCCG ACTGTTGGTC AGACCAGCCC CCGAACCCGG
    AAGTCCATGA GTTTGGATAT GGGACAGCCT TCCCAGGCCA ACACTAAGAA GCTGCTTGGA
    ACCAGGAAAA GCTTTGACCA CTTGATATCA GACACAAAGG CTCCTAAAAG GCAAGAAATG
    GAATCTGGGA TCACCACCCC TCCCAAAATG AGGAGAGTGG CCGAGAATGA CTATGAAATG
    GAAACCCAGA GAATCTCCTC ACCTCAACAG CACCCACACC TGCGCAAAGT TTCTGTGTCT
    GAATCAAATG TACTTCTTGA TGAAGAAGTT CTAACCGATC CCAAAATCCA AGCCCTGCTG
    CTTACTGTCC TGGCTACACT GGTAAAATAC ACTACAGACG AGTTTGATCA ACGGATCCTT
    TATGAATATT TAGCCGAGGC CAGTGTTGTC TTCCCTAAAG TTTTTCCAGT TGTGCACAAT
    CTGTTGGACT CCAAGATTAA TACCCTTCTG TCGCTATGCC AAGATCCAAA TCTACTGAAT
    CCAATTCATG GGATCGTGCA GAGCGTGGTC TACCATGAAG AGTCGCCACC ACAGTACCAA
    ACCTCTTATC TACAAAGTTT TGGTTTTAAT GGCTTGTGGA GGTTTGCGGG ACCATTTTCT
    AAGCAAACCC AAATTCCAGA CTATGCTGAG CTGATTGTGA AATTTCTCGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA GCCAGTGAGG AGTCCTTACT GACCCCCACC
    TCTCCTTATC CTCCAGCAGT GCAGAGCCAG CTGAGCATTA CTGCTAATCT TAACCTTTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GCATTGACAA GGAGAATGTG
    GAGCTCTCTC CGACCACTGG CCACTCCAAC AGTGGGAGGA CACGCCACGG CTCCGCAAGC
    CAAGTGCAAA AGCAGCGAAG TGCCGGCAGT TTCAAACGTC ACAGTATCAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028938467.1
    CDS43..8565
    Translation

    Target ORF information:

    RefSeq Version XM_029082634.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029082634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAAGCCGGT GGAGTGGGTC CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AAGCCGGACA GCAGAGCACG CACACCAAAG TCAGTACTGA ACACAACAAG
    GAGTGTCTTA TCAACATTTC TAAATACAAG TTTTCTTTGG TTATAAGTGG TCTCACCAAT
    ATATTAAAAA ATGTTAATAA TATGAGAATA TTTGGAGAAG CTGCCGAAAA GAATTTGTAT
    CTCTCTCAAT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC
    ACCATGCGAT TGGATGAGAC TATGCTGGTC AAGCAGTTGC TGCCGGAAAT CTGCCATTTT
    ATCCACACCT ATCGGGAAGG AAACCAACAT GCAGCTGAGC TGCGGAATTC TGCCTCGGGA
    GTTCTCTTCT CTCTCAGCTG TAATAACTTC AATGCAGTCT TCAGCCGTAT TTCCACCAGG
    TTACAGGAGC TGACTGTGTG CTCAGAAGAT AATGTTGATG TCCATGATAT AGAATTGTTA
    CAGTACATCA ATGTGGACTG TGCAAAGCTG AAGCGACTGC TGCAGGAAAC AGTATTTAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGCTG GCAGTTATAA ACAGTCTGGA AAAAGCATTT
    TGGAACTGGG TAGAGAACTA TCCTGATGAA TTTACAAAGT TGTACCAGAC TCCCCAGACA
    GACATGGCTG ATTGTGCCGA AAAGTTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACG
    AAACGTAAAG CAGCAGTTTG GCCATTGCAA ATCATTCTTC TTGTCCTGTG CCCAGAAATA
    ATCCAAGATA TATCCAAGGA TGTGGTGGAA GAAACCAAAA TAAATAAGAA GTTGTTTCTG
    GAAAATCTCA GAAAAGCACT CGCTGGCCAT GGTGGAAGTA GGCAACTGAC TGAAAGTGCT
    GCTATTGCTT GTGTCAAATT GTGCAAAGCA AGTACCTACA TCAACTGGGA AGATAACTCT
    GTCATTTTCC TATTAGTACA GTCCATGGTG GTAGATCTTA AGAACCTGCT GTTTAATCCA
    ACTAAACCCT TCTCAAGAGG CAATCAGAAT GCAGATGTGG ATCTAATGAT TGACTGTTTA
    GTTTCTTGTT TTCGCATAAA CCCTCACAAC AACCAACACT TTAAGATCTG CTTGGCCCAG
    AGTTCTCCAT CCACATTTCA TTACGTGCTG GTAAATTCAC TCCATCGAAT CATCACAAAT
    TCTGCTCTGG ATTGGTGGCC CAAGATTGAT GCGGTGTACT GCCATTCAGT TGAACTCCGC
    AGCATGTTCG GTGAAACGCT TCACAAAGCA GTGCAGGGCT GTGGAGCCCA TCCAGCAATA
    CGAATGGCCA CGAGTCTTAC ATTCAAAGAA AAAATGACAA GTCTTAAATT TAAAGAAAAA
    CCTACAGACT TGGAAACAAG AAGTTATAAA TATCTCCTGT TGTCCATGGT GAAACTAATC
    CATGCCGATC CAAAGCTGTT GCTCTGTAAT CCGAGGAAAC AAGGGCCCGA AACCCAGGGC
    AGCACAGCAG AGTTAATCAC AGGACTTGTC CAGCTTGTTC CCCAGTCAAA CATGCCAGAA
    ATAGCCCAAG AAGCCATGGA GGCTTTGTTG GTTCTTCATC AATTAGAGAG TATTGATTTA
    TGGAATCCTG ATGCACCTAT AGAAACATTT TGGGAGATTA GTTCGCAAAT GCTTTTTTAC
    ATCTGTAAGA AGCTAACCAG TCACCAGATG CTTAGCAGCA CAGAAATTCT CAAATGGCTG
    CGGGAAATTT TGATCTGCAG GAATAAATTC CTTCTTAAAA ATAAGCAGTC AGATAGGAGT
    TCCTGCCACT TCCTTTTCCT TTATGGGGTA GCATGTGACG TCTCTTCTGG TGGGGGTGCT
    AGTCAAATCT CTCTAGACCA TGAAGAGTTA ATACGCACCT GTACTCCGGG AGCATCCCTC
    AGGAAGGGGA AAGGAAACTC ATCCATGGAC AGTACAGTCG GATGCAGCGG AACTCCAATA
    TGCCGCCAAG CTCAGACTAA GCTAGAAGTT GCTCTCTACA TGTTCCTCTG GAGCCCCGAC
    ATTGAGGCCG TCCTTGTCGC CATGTCCTGT TTCCGCCACC TCTGTGAAGA AGCTGACATT
    AGGTGTGGGG TAGATGAAGT TTCCGTGCAT AATTTCTTGC CAAACTACAA TACCTTCATG
    GAGTTTGCCT CCGTCAGCAA TATGATGTCT ACAGGAAGAG CGGCACTTCA AAAGAGGGTG
    ATGGCACTGT TGAGACGAAT TGAACATCCA ACTGCCGGCA ACACTGAGGC ATGGGAAGAC
    ACTCATACAA AATGGGAACA AGCTACGAAG CTGATCCTTA ACTATCCAAA AGCCAAAATG
    GAAGATGGCC AGGTAACCGA AAGTCTTCAC AAGACCATTG TAAAGCGGCG AATGTCTCAC
    GTGAGCGGGG GAGGATCCAT CGATTTGTCT GACACGGACT CCCTGCAGGA GTGGATCAAT
    ATGACGGGCT TCCTTTGTGC TCTCGGTGGG GTGTGCCTGC AGCACCGTGG CAACGCGGGC
    TTGGCCACCT ACAGCCCACC TATGGGCCCA GTCAGTGAGC GAAAGGGTTC CATGATTTCT
    ATGGTATCCA CCGAAGGAAA TACCGAGACG CCCGTCAGCA AGTTCATGGA CCGGTTGCTC
    TCCCTGATGG TGTGCAACCA CGAGAAAGTG GGACTCCAAA TACGGACGAA TATCAAGGAC
    CTGGTGGGTC TCGAATTAAG TCCTGCACTT TATCCGATGC TATTCAACAA GTTGAAGAAC
    ACCATCAGCA AATTTTTTGA CTCTCAAGGG CAGGTGTTGC TGAATGATAC CAATACCCAA
    TTTGTAGAGC AGACCATAGC GATAATGAAG AACTTGCTGG ATAATCACAC CGAAGGCAGC
    TCGGAGCACC TGGGGCAAGC CAGCATAGAA ACAATGATGC TCAATCTAGT CAGGTACGTT
    CGTGTGCTTG GGAATTTGGT ACACGCAATT CAGATTAAAA CGAAACTGTG CCAGTTAGTT
    GAAGTAATGA TGGAGAGGCG AGATGATCTT TCGTTTTGCC AAGAAATGAA ATTTAGGAAC
    AAGATGGTGG AATATCTGAC AGATTGGGTC ATGGGGACAT CGAACCAAGC AACAGATGAA
    GATGTAAAGT GTTTAACAAG AGATTTGGAC CAGGCAAGCA TGGAAGCAGT TGTGTCTCTT
    CTTGCTGGAC TCCCCCTGCA GCCGGAAGAA GGAGATGGTG TGGAATTGAT GGAAGCCAAG
    TCCCAGTTAT TTCTTAAGTA TTTCACTTTG TTTATGAACC TTCTCAATGA CTGCAGTGAG
    GTTGAAGATG AAAACGCCCA AGCGGGAGGC AGGAAACGTG GGATGTCTCG AAGACTGGCT
    TCCCTGAGGC ACTGTACCGT GCTTGCTATG TCAAACTTAC TCAACGCTAA CGTGGACAGC
    GGCCTTATGC ATTCCATAGG TTTAGGTTAT CACAAAGATC TCCAAACGAG AGCCACGTTC
    ATGGAAGTCC TCACGAAAAT TCTCCAGCAG GGCACCGAGT TTGACACGTT AGCGGAGACC
    GTATTGGCCG ATCGATTTGA GAGGCTGGTG GAATTAGTCA CGATGATGGG TGATCAAGGA
    GAGCTTCCCA TAGCAATGGC CCTGGCCAAC GTGGTACCTT GTTCACAATG GGATGAGTTA
    GCTCGGGTTC TCGTCACTCT CTTTGATTCA CGCCACTTAC TCTACCAGCT GCTTTGGAAC
    ATGTTTTCTA AGGAAGTTGA GCTGGCAGAT TCCATGCAGA CTCTCTTCAG GGGGAACAGT
    CTGGCCAGTA AAATAATGAC ATTCTGCTTT AAGGTTTACG GTGCTACATA TCTGCAAAAG
    CTTTTGGAAC CTTTACTGAG GACTGTCATC ACGTCGATGG AATGGCAGCA CGTCAGCTTT
    GAGGTGGATC CTACAAGGTT AGAGCCTACA GAGAGCCTTG ATGATAACCA ACGGAGTCTC
    TTACAAATGA CTGAAAAGTT CTTCCATGCT ATCATCAATT CCTCCTCAGA GTTTCCCCCT
    CAGCTTCGCA GTGTGTGCCA TTGTTTGTAC CAGGCAACTT GCCACTCTCT ACTGAATAAA
    GCTACAGTAA AAGAAAAAAA GGAAAACAAA AAATCAGTGG TTAGCCAGCG TTTTCCTCAG
    AACAGTATTG GTGCAGTAGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AGGCTGGGAT CTTAGATAAA AAGCCTCCGC CTAGAATTGA GAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TCCTGTTCAC AAAAGAAGAG
    CATATGAGGC CTTTTAATGA CTTTGTGAAA AGCAACTTCG ATGCAGCCCG AAGGTTTTTC
    CTCGATATAG CGTCTGATTG TCCTGCAAGC GATTCAGTAA ATCACAGCCT CTCCTTCATC
    AGTGATGGCA ATGTACTTGC TTTACATCGT TTGCTCTGGA ACAACCAAGA AAAGATCGGC
    CAATATCTTT CCAGCAACAG GGACCACAAG GCTGTTGGAC GTCGACCCTT TGACAAGATG
    GCTACACTCC TTGCGTATCT GGGCCCTCCT GAGCACAAGC CCGTAGCAGA TACCCACTGG
    TCCAGCCTGA ATCTGACCAG TTCCAAATTT GAGGAGTTCA TGACCAGACA CCAGGTACAT
    GAAAAAGAGG AATTTAAAGC ATTGAAAACA TTAAATATCT TCTATCAAGC AGGGACTTCC
    AAGGCCGGGA ATCCAGTTTT TTATTACATT GCACGGAGGT TCAAGACCGG TCAGATCAAT
    GGGGATTTGC TGATATACCA CGTCTTGCTG ACTCTCAAGC CATATTATGC CAAGCCATAT
    GAGATCGTCG TGGACCTTAC CCACGCCGGC CCCAGCAATC GCTTTAAAAC AGACTTTCTC
    TCCAAGTGGT TTGTCGTTTT CCCTGGCTTT GCGTACGAAA ATGTGTCTGC TGTTTACATC
    TACAACTGTA ATTCTTGGGT CAGGGAGTAC ACCAAATACC ACGAGAGACT GCTCACTGGC
    CTCAAAGGCA GCAAGAGACT TATTTTCATA GACTCGCCTG GAAAATTGGC TGAGCACATC
    GAGCACGACC AGCAGAAACT GCCGGCTGCC ACTTTGGCTT TAGAAGAGGA TTTGAAGGTG
    TTCCACAATG CTCTCAAACT GGCCCACAAA GACACCAAGG TTTCTATTAA AGTTGGCTCG
    ACTGCCGTTC AGGTCACATC AGCCGAACGG ACAAGGGTCT TGGGGCAGTC GGTTTTCCTG
    AACGACATCT ATTACGCCTC AGAGATCGAG GAGATCTGCC TCGTTGACGA AAACCAGTTC
    ACTTTAACCA TTGCTAACCA AGGCACTCCA CTCACCTTCA TGCACCAAGA GTGCGAAGCC
    ATCGTTCAAT CCATCATCCA CATACGGACA CGGTGGGAGC TGTCTCAACC CGACTCCATC
    CCCCAGCACA CGAAAATCCG GCCGAAGGAT GTCCCCGGAA CGCTCCTGAA TATCGCGTTG
    CTTAATCTGG GCAGCTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACCTGTA CCTTTAATTT AAAGATTGAA GGCCAGTTGC TAGAGACTTC AGGTCTGTGT
    ATCCCTGCCA ACAACACCCT TTTTATTGTC TCTATTAGTA AGACCTTGGC AGCTAATGAG
    CCACACCTCA CCCTGGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAGCTGAAGC ACCTTTGCTT AGAATATATG ACTCCCTGGC TATCAAATCT AGTGCGTTTC
    TGTAAACACA ATGATGATGC CAAACGCCAG CGAGTCACTG CTATTCTTGA CAAGCTCATA
    ACAATGACGA TTAATGAGAA ACAGATGTAT CCATCTATCC AAGCCAAGAT CTGGGGAAGT
    CTTGGACAGA TTACAGATCT GCTTGATGTC GTGCTGGACA GTTTCATCAA AACCAGTGCA
    ACTGGTGGCT TGGGCTCTAT AAAGGCTGAG GTGATGGCAG ACACAGCTGT GGCTCTTGCT
    TCCGGAAATG TGAAACTCGT TTCGAGTAAG GTCATCGGGA GAATGTGCAA AATAATCGAC
    AAGACCTGCC TGTCTCCAAC TCCCACCTTA GAACAACACC TCATGTGGGA CGATATTGCC
    ATCCTTGCCC GATACATGCT GATGCTGTCC TTTAACAACT CTCTCGACGT GGCAGCCCAC
    CTGCCCTATC TCTTCCATGT TGTTACTCTG TTGGTGGCCA CTGGTCCTCT GTCCCTTAGA
    GCTTCCACTC ACGGACTGGT CATCAACATC ATTCATTCTC TGTGCACTTG TTCACAGCTT
    CATTTCAGTG AGGAGACCAA GCAAGTTTTG AGGCTCAGCT TGACAGAGTT CTCATTACCC
    AAGTTTTATT TGCTGTTTGG CATCAGCAAA GTCAAGTCGG CAGCTGTCAT AGCCTTCCGG
    TCCAGTTACC GGGACAGGTC TTTCTCACCT GGTTCCTACG AGAGGGAGAC GTTTGCCTTG
    ACATCCCTGG AGACCGTGAC AGAGGCTCTA CTGGAGATTA TGGAGGCTTG TATGAGAGAT
    ATTCCAACGT GCAAGTGGCT GGACCAATGG ACAGAACTAG CACAAAAATT TGCATTCCAG
    TATAATCCAT CACTCCAGCC AAGAGCCCTC GTTGTCTTCG GTTGTATCAG CAAAAGGGTA
    TCTCACGGGC AGATAAAACA AATTATCCGT ATTCTTAGCA AGGGACTTGA GAGCTGTCTA
    AAGGGACCTG ATAATTACAA CAGTCAAGTT CTGATAGAAG CCACAGTAAT AGCACTGACC
    AAATTACAGC CACTTTTGAA TAAGGATTCT CCTATGCACA AAGCCCTCTT TTGGGTGGCT
    ATGGCTGTGT TGCAGCTGGA TGAAGTTAAC TTGTATTCAG CTGGGACAGC ACTTCTTGAG
    CAGAACCTGC ACACGTTAGA TAGTCTCCGG GTATTCAATG ACAAGAGTCC AGAAGAAGTC
    TTCATGGAAA TCCGAAATCC GCTTGAATGG CACTGCAAAC AAATGGATCA TTTTGTTGGA
    CTCAATTTTA ACTCTAATTT TAATTTTGCA CTAGTAGGAC ACCTTTTAAA AGGATACAGG
    CATCCTTCTC CTATCATTGT TGCAAGAACA GTAAGGATCT TACACACACT ACTAGCTCTG
    GTTAACAAAC ATAGGAACTG TGACAAATTT GAGGTGAATA CCCAGAGTGT GGCTTACTTA
    GCAGCTTTGC TTACAGTGTC TGAGGAAGTG CGAAGTCGCT GTAGCCTAAA ACATAGGAAA
    TCACTGCTGC TGACAGATGT CTCTATGGAA AATGTTCCTA TGGATACATA TCCCATCCAT
    CACAACGATC CCAGCTGTAG GACACTAAAG GAAAATCAAC CATGGTCCTC CCCAAAGGGC
    TCCGAGAGAC ATCTCGCCGC CAGCTACCCG ACTGTTGGTC AGACCAGCCC CCGAACCCGG
    AAGTCCATGA GTTTGGATAT GGGACAGCCT TCCCAGGCCA ACACTAAGAA GCTGCTTGGA
    ACCAGGAAAA GCTTTGACCA CTTGATATCA GACACAAAGG CTCCTAAAAG GCAAGAAATG
    GAATCTGGGA TCACCACCCC TCCCAAAATG AGGAGAGTGG CCGAGAATGA CTATGAAATG
    GAAACCCAGA GAATCTCCTC ACCTCAACAG CACCCACACC TGCGCAAAGT TTCTGTGTCT
    GAATCAAATG TACTTCTTGA TGAAGAAGTT CTAACCGATC CCAAAATCCA AGCCCTGCTG
    CTTACTGTCC TGGCTACACT GGTAAAATAC ACTACAGACG AGTTTGATCA ACGGATCCTT
    TATGAATATT TAGCCGAGGC CAGTGTTGTC TTCCCTAAAG TTTTTCCAGT TGTGCACAAT
    CTGTTGGACT CCAAGATTAA TACCCTTCTG TCGCTATGCC AAGATCCAAA TCTACTGAAT
    CCAATTCATG GGATCGTGCA GAGCGTGGTC TACCATGAAG AGTCGCCACC ACAGTACCAA
    ACCTCTTATC TACAAAGTTT TGGTTTTAAT GGCTTGTGGA GGTTTGCGGG ACCATTTTCT
    AAGCAAACCC AAATTCCAGA CTATGCTGAG CTGATTGTGA AATTTCTCGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA GCCAGTGAGG AGTCCTTACT GACCCCCACC
    TCTCCTTATC CTCCAGCAGT GCAGAGCCAG CTGAGCATTA CTGCTAATCT TAACCTTTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GCATTGACAA GGAGAATGTG
    GAGCTCTCTC CGACCACTGG CCACTCCAAC AGTGGGAGGA CACGCCACGG CTCCGCAAGC
    CAAGTGCAAA AGCAGCGAAG TGCCGGCAGT TTCAAACGTC ACAGTATCAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc119299
    Clone ID Related Accession (Same CDS sequence) XM_029082636.1
    Accession Version XM_029082636.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041744.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGGAGTCC AAAAACGTAG GTTCTGCCCC TCACTACGCC GCTTGTCTGC TGTGGGACCT 
    TGGCTTCCAA TAAAAGCCGG ACAGCAGAGC ACGCACACCA AAGTCAGTAC TGAACACAAC
    AAGGAGTGTC TTATCAACAT TTCTAAATAC AAGTTTTCTT TGGTTATAAG TGGTCTCACC
    AATATATTAA AAAATGTTAA TAATATGAGA ATATTTGGAG AAGCTGCCGA AAAGAATTTG
    TATCTCTCTC AATTGATTAT ATTGGATACA CTGGAAAAAT GTCTTGCCGG GCAACCAAAG
    GACACCATGC GATTGGATGA GACTATGCTG GTCAAGCAGT TGCTGCCGGA AATCTGCCAT
    TTTATCCACA CCTATCGGGA AGGAAACCAA CATGCAGCTG AGCTGCGGAA TTCTGCCTCG
    GGAGTTCTCT TCTCTCTCAG CTGTAATAAC TTCAATGCAG TCTTCAGCCG TATTTCCACC
    AGGTTACAGG AGCTGACTGT GTGCTCAGAA GATAATGTTG ATGTCCATGA TATAGAATTG
    TTACAGTACA TCAATGTGGA CTGTGCAAAG CTGAAGCGAC TGCTGCAGGA AACAGTATTT
    AAGTTTAAAG CCCTTAAGAA AGTAGCTCAG CTGGCAGTTA TAAACAGTCT GGAAAAAGCA
    TTTTGGAACT GGGTAGAGAA CTATCCTGAT GAATTTACAA AGTTGTACCA GACTCCCCAG
    ACAGACATGG CTGATTGTGC CGAAAAGTTG TTTGACTTGG TGGATGGTTT TGCTGAAAGC
    ACGAAACGTA AAGCAGCAGT TTGGCCATTG CAAATCATTC TTCTTGTCCT GTGCCCAGAA
    ATAATCCAAG ATATATCCAA GGATGTGGTG GAAGAAACCA AAATAAATAA GAAGTTGTTT
    CTGGAAAATC TCAGAAAAGC ACTCGCTGGC CATGGTGGAA GTAGGCAACT GACTGAAAGT
    GCTGCTATTG CTTGTGTCAA ATTGTGCAAA GCAAGTACCT ACATCAACTG GGAAGATAAC
    TCTGTCATTT TCCTATTAGT ACAGTCCATG GTGGTAGATC TTAAGAACCT GCTGTTTAAT
    CCAACTAAAC CCTTCTCAAG AGGCAATCAG AATGCAGATG TGGATCTAAT GATTGACTGT
    TTAGTTTCTT GTTTTCGCAT AAACCCTCAC AACAACCAAC ACTTTAAGAT CTGCTTGGCC
    CAGAGTTCTC CATCCACATT TCATTACGTG CTGGTAAATT CACTCCATCG AATCATCACA
    AATTCTGCTC TGGATTGGTG GCCCAAGATT GATGCGGTGT ACTGCCATTC AGTTGAACTC
    CGCAGCATGT TCGGTGAAAC GCTTCACAAA GCAGTGCAGG GCTGTGGAGC CCATCCAGCA
    ATACGAATGG CCACGAGTCT TACATTCAAA GAAAAAATGA CAAGTCTTAA ATTTAAAGAA
    AAACCTACAG ACTTGGAAAC AAGAAGTTAT AAATATCTCC TGTTGTCCAT GGTGAAACTA
    ATCCATGCCG ATCCAAAGCT GTTGCTCTGT AATCCGAGGA AACAAGGGCC CGAAACCCAG
    GGCAGCACAG CAGAGTTAAT CACAGGACTT GTCCAGCTTG TTCCCCAGTC AAACATGCCA
    GAAATAGCCC AAGAAGCCAT GGAGGCTTTG TTGGTTCTTC ATCAATTAGA GAGTATTGAT
    TTATGGAATC CTGATGCACC TATAGAAACA TTTTGGGAGA TTAGTTCGCA AATGCTTTTT
    TACATCTGTA AGAAGCTAAC CAGTCACCAG ATGCTTAGCA GCACAGAAAT TCTCAAATGG
    CTGCGGGAAA TTTTGATCTG CAGGAATAAA TTCCTTCTTA AAAATAAGCA GTCAGATAGG
    AGTTCCTGCC ACTTCCTTTT CCTTTATGGG GTAGCATGTG ACGTCTCTTC TGGTGGGGGT
    GCTAGTCAAA TCTCTCTAGA CCATGAAGAG TTAATACGCA CCTGTACTCC GGGAGCATCC
    CTCAGGAAGG GGAAAGGAAA CTCATCCATG GACAGTACAG TCGGATGCAG CGGAACTCCA
    ATATGCCGCC AAGCTCAGAC TAAGCTAGAA GTTGCTCTCT ACATGTTCCT CTGGAGCCCC
    GACATTGAGG CCGTCCTTGT CGCCATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCTGAC
    ATTAGGTGTG GGGTAGATGA AGTTTCCGTG CATAATTTCT TGCCAAACTA CAATACCTTC
    ATGGAGTTTG CCTCCGTCAG CAATATGATG TCTACAGGAA GAGCGGCACT TCAAAAGAGG
    GTGATGGCAC TGTTGAGACG AATTGAACAT CCAACTGCCG GCAACACTGA GGCATGGGAA
    GACACTCATA CAAAATGGGA ACAAGCTACG AAGCTGATCC TTAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGTAAC CGAAAGTCTT CACAAGACCA TTGTAAAGCG GCGAATGTCT
    CACGTGAGCG GGGGAGGATC CATCGATTTG TCTGACACGG ACTCCCTGCA GGAGTGGATC
    AATATGACGG GCTTCCTTTG TGCTCTCGGT GGGGTGTGCC TGCAGCACCG TGGCAACGCG
    GGCTTGGCCA CCTACAGCCC ACCTATGGGC CCAGTCAGTG AGCGAAAGGG TTCCATGATT
    TCTATGGTAT CCACCGAAGG AAATACCGAG ACGCCCGTCA GCAAGTTCAT GGACCGGTTG
    CTCTCCCTGA TGGTGTGCAA CCACGAGAAA GTGGGACTCC AAATACGGAC GAATATCAAG
    GACCTGGTGG GTCTCGAATT AAGTCCTGCA CTTTATCCGA TGCTATTCAA CAAGTTGAAG
    AACACCATCA GCAAATTTTT TGACTCTCAA GGGCAGGTGT TGCTGAATGA TACCAATACC
    CAATTTGTAG AGCAGACCAT AGCGATAATG AAGAACTTGC TGGATAATCA CACCGAAGGC
    AGCTCGGAGC ACCTGGGGCA AGCCAGCATA GAAACAATGA TGCTCAATCT AGTCAGGTAC
    GTTCGTGTGC TTGGGAATTT GGTACACGCA ATTCAGATTA AAACGAAACT GTGCCAGTTA
    GTTGAAGTAA TGATGGAGAG GCGAGATGAT CTTTCGTTTT GCCAAGAAAT GAAATTTAGG
    AACAAGATGG TGGAATATCT GACAGATTGG GTCATGGGGA CATCGAACCA AGCAACAGAT
    GAAGATGTAA AGTGTTTAAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTTGTGTCT
    CTTCTTGCTG GACTCCCCCT GCAGCCGGAA GAAGGAGATG GTGTGGAATT GATGGAAGCC
    AAGTCCCAGT TATTTCTTAA GTATTTCACT TTGTTTATGA ACCTTCTCAA TGACTGCAGT
    GAGGTTGAAG ATGAAAACGC CCAAGCGGGA GGCAGGAAAC GTGGGATGTC TCGAAGACTG
    GCTTCCCTGA GGCACTGTAC CGTGCTTGCT ATGTCAAACT TACTCAACGC TAACGTGGAC
    AGCGGCCTTA TGCATTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAAAC GAGAGCCACG
    TTCATGGAAG TCCTCACGAA AATTCTCCAG CAGGGCACCG AGTTTGACAC GTTAGCGGAG
    ACCGTATTGG CCGATCGATT TGAGAGGCTG GTGGAATTAG TCACGATGAT GGGTGATCAA
    GGAGAGCTTC CCATAGCAAT GGCCCTGGCC AACGTGGTAC CTTGTTCACA ATGGGATGAG
    TTAGCTCGGG TTCTCGTCAC TCTCTTTGAT TCACGCCACT TACTCTACCA GCTGCTTTGG
    AACATGTTTT CTAAGGAAGT TGAGCTGGCA GATTCCATGC AGACTCTCTT CAGGGGGAAC
    AGTCTGGCCA GTAAAATAAT GACATTCTGC TTTAAGGTTT ACGGTGCTAC ATATCTGCAA
    AAGCTTTTGG AACCTTTACT GAGGACTGTC ATCACGTCGA TGGAATGGCA GCACGTCAGC
    TTTGAGGTGG ATCCTACAAG GTTAGAGCCT ACAGAGAGCC TTGATGATAA CCAACGGAGT
    CTCTTACAAA TGACTGAAAA GTTCTTCCAT GCTATCATCA ATTCCTCCTC AGAGTTTCCC
    CCTCAGCTTC GCAGTGTGTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG TTTTCCTCAG
    AACAGTATTG GTGCAGTAGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AGGCTGGGAT CTTAGATAAA AAGCCTCCGC CTAGAATTGA GAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TCCTGTTCAC AAAAGAAGAG
    CATATGAGGC CTTTTAATGA CTTTGTGAAA AGCAACTTCG ATGCAGCCCG AAGGTTTTTC
    CTCGATATAG CGTCTGATTG TCCTGCAAGC GATTCAGTAA ATCACAGCCT CTCCTTCATC
    AGTGATGGCA ATGTACTTGC TTTACATCGT TTGCTCTGGA ACAACCAAGA AAAGATCGGC
    CAATATCTTT CCAGCAACAG GGACCACAAG GCTGTTGGAC GTCGACCCTT TGACAAGATG
    GCTACACTCC TTGCGTATCT GGGCCCTCCT GAGCACAAGC CCGTAGCAGA TACCCACTGG
    TCCAGCCTGA ATCTGACCAG TTCCAAATTT GAGGAGTTCA TGACCAGACA CCAGGTACAT
    GAAAAAGAGG AATTTAAAGC ATTGAAAACA TTAAATATCT TCTATCAAGC AGGGACTTCC
    AAGGCCGGGA ATCCAGTTTT TTATTACATT GCACGGAGGT TCAAGACCGG TCAGATCAAT
    GGGGATTTGC TGATATACCA CGTCTTGCTG ACTCTCAAGC CATATTATGC CAAGCCATAT
    GAGATCGTCG TGGACCTTAC CCACGCCGGC CCCAGCAATC GCTTTAAAAC AGACTTTCTC
    TCCAAGTGGT TTGTCGTTTT CCCTGGCTTT GCGTACGAAA ATGTGTCTGC TGTTTACATC
    TACAACTGTA ATTCTTGGGT CAGGGAGTAC ACCAAATACC ACGAGAGACT GCTCACTGGC
    CTCAAAGGCA GCAAGAGACT TATTTTCATA GACTCGCCTG GAAAATTGGC TGAGCACATC
    GAGCACGACC AGCAGAAACT GCCGGCTGCC ACTTTGGCTT TAGAAGAGGA TTTGAAGGTG
    TTCCACAATG CTCTCAAACT GGCCCACAAA GACACCAAGG TTTCTATTAA AGTTGGCTCG
    ACTGCCGTTC AGGTCACATC AGCCGAACGG ACAAGGGTCT TGGGGCAGTC GGTTTTCCTG
    AACGACATCT ATTACGCCTC AGAGATCGAG GAGATCTGCC TCGTTGACGA AAACCAGTTC
    ACTTTAACCA TTGCTAACCA AGGCACTCCA CTCACCTTCA TGCACCAAGA GTGCGAAGCC
    ATCGTTCAAT CCATCATCCA CATACGGACA CGGTGGGAGC TGTCTCAACC CGACTCCATC
    CCCCAGCACA CGAAAATCCG GCCGAAGGAT GTCCCCGGAA CGCTCCTGAA TATCGCGTTG
    CTTAATCTGG GCAGCTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACCTGTA CCTTTAATTT AAAGATTGAA GGCCAGTTGC TAGAGACTTC AGGTCTGTGT
    ATCCCTGCCA ACAACACCCT TTTTATTGTC TCTATTAGTA AGACCTTGGC AGCTAATGAG
    CCACACCTCA CCCTGGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAGCTGAAGC ACCTTTGCTT AGAATATATG ACTCCCTGGC TATCAAATCT AGTGCGTTTC
    TGTAAACACA ATGATGATGC CAAACGCCAG CGAGTCACTG CTATTCTTGA CAAGCTCATA
    ACAATGACGA TTAATGAGAA ACAGATGTAT CCATCTATCC AAGCCAAGAT CTGGGGAAGT
    CTTGGACAGA TTACAGATCT GCTTGATGTC GTGCTGGACA GTTTCATCAA AACCAGTGCA
    ACTGGTGGCT TGGGCTCTAT AAAGGCTGAG GTGATGGCAG ACACAGCTGT GGCTCTTGCT
    TCCGGAAATG TGAAACTCGT TTCGAGTAAG GTCATCGGGA GAATGTGCAA AATAATCGAC
    AAGACCTGCC TGTCTCCAAC TCCCACCTTA GAACAACACC TCATGTGGGA CGATATTGCC
    ATCCTTGCCC GATACATGCT GATGCTGTCC TTTAACAACT CTCTCGACGT GGCAGCCCAC
    CTGCCCTATC TCTTCCATGT TGTTACTCTG TTGGTGGCCA CTGGTCCTCT GTCCCTTAGA
    GCTTCCACTC ACGGACTGGT CATCAACATC ATTCATTCTC TGTGCACTTG TTCACAGCTT
    CATTTCAGTG AGGAGACCAA GCAAGTTTTG AGGCTCAGCT TGACAGAGTT CTCATTACCC
    AAGTTTTATT TGCTGTTTGG CATCAGCAAA GTCAAGTCGG CAGCTGTCAT AGCCTTCCGG
    TCCAGTTACC GGGACAGGTC TTTCTCACCT GGTTCCTACG AGAGGGAGAC GTTTGCCTTG
    ACATCCCTGG AGACCGTGAC AGAGGCTCTA CTGGAGATTA TGGAGGCTTG TATGAGAGAT
    ATTCCAACGT GCAAGTGGCT GGACCAATGG ACAGAACTAG CACAAAAATT TGCATTCCAG
    TATAATCCAT CACTCCAGCC AAGAGCCCTC GTTGTCTTCG GTTGTATCAG CAAAAGGGTA
    TCTCACGGGC AGATAAAACA AATTATCCGT ATTCTTAGCA AGGGACTTGA GAGCTGTCTA
    AAGGGACCTG ATAATTACAA CAGTCAAGTT CTGATAGAAG CCACAGTAAT AGCACTGACC
    AAATTACAGC CACTTTTGAA TAAGGATTCT CCTATGCACA AAGCCCTCTT TTGGGTGGCT
    ATGGCTGTGT TGCAGCTGGA TGAAGTTAAC TTGTATTCAG CTGGGACAGC ACTTCTTGAG
    CAGAACCTGC ACACGTTAGA TAGTCTCCGG GTATTCAATG ACAAGAGTCC AGAAGAAGTC
    TTCATGGAAA TCCGAAATCC GCTTGAATGG CACTGCAAAC AAATGGATCA TTTTGTTGGA
    CTCAATTTTA ACTCTAATTT TAATTTTGCA CTAGTAGGAC ACCTTTTAAA AGGATACAGG
    CATCCTTCTC CTATCATTGT TGCAAGAACA GTAAGGATCT TACACACACT ACTAGCTCTG
    GTTAACAAAC ATAGGAACTG TGACAAATTT GAGGTGAATA CCCAGAGTGT GGCTTACTTA
    GCAGCTTTGC TTACAGTGTC TGAGGAAGTG CGAAGTCGCT GTAGCCTAAA ACATAGGAAA
    TCACTGCTGC TGACAGATGT CTCTATGGAA AATGTTCCTA TGGATACATA TCCCATCCAT
    CACAACGATC CCAGCTGTAG GACACTAAAG GAAAATCAAC CATGGTCCTC CCCAAAGGGC
    TCCGAGAGAC ATCTCGCCGC CAGCTACCCG ACTGTTGGTC AGACCAGCCC CCGAACCCGG
    AAGTCCATGA GTTTGGATAT GGGACAGCCT TCCCAGGCCA ACACTAAGAA GCTGCTTGGA
    ACCAGGAAAA GCTTTGACCA CTTGATATCA GACACAAAGG CTCCTAAAAG GCAAGAAATG
    GAATCTGGGA TCACCACCCC TCCCAAAATG AGGAGAGTGG CCGAGAATGA CTATGAAATG
    GAAACCCAGA GAATCTCCTC ACCTCAACAG CACCCACACC TGCGCAAAGT TTCTGTGTCT
    GAATCAAATG TACTTCTTGA TGAAGAAGTT CTAACCGATC CCAAAATCCA AGCCCTGCTG
    CTTACTGTCC TGGCTACACT GGTAAAATAC ACTACAGACG AGTTTGATCA ACGGATCCTT
    TATGAATATT TAGCCGAGGC CAGTGTTGTC TTCCCTAAAG TTTTTCCAGT TGTGCACAAT
    CTGTTGGACT CCAAGATTAA TACCCTTCTG TCGCTATGCC AAGATCCAAA TCTACTGAAT
    CCAATTCATG GGATCGTGCA GAGCGTGGTC TACCATGAAG AGTCGCCACC ACAGTACCAA
    ACCTCTTATC TACAAAGTTT TGGTTTTAAT GGCTTGTGGA GGTTTGCGGG ACCATTTTCT
    AAGCAAACCC AAATTCCAGA CTATGCTGAG CTGATTGTGA AATTTCTCGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA GCCAGTGAGG AGTCCTTACT GACCCCCACC
    TCTCCTTATC CTCCAGCAGT GCAGAGCCAG CTGAGCATTA CTGCTAATCT TAACCTTTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GCATTGACAA GGAGAATGTG
    GAGCTCTCTC CGACCACTGG CCACTCCAAC AGTGGGAGGA CACGCCACGG CTCCGCAAGC
    CAAGTGCAAA AGCAGCGAAG TGCCGGCAGT TTCAAACGTC ACAGTATCAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028938469.1
    CDS16..8478
    Translation

    Target ORF information:

    RefSeq Version XM_029082636.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029082636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGGAGTCC AAAAACGTAG GTTCTGCCCC TCACTACGCC GCTTGTCTGC TGTGGGACCT 
    TGGCTTCCAA TAAAAGCCGG ACAGCAGAGC ACGCACACCA AAGTCAGTAC TGAACACAAC
    AAGGAGTGTC TTATCAACAT TTCTAAATAC AAGTTTTCTT TGGTTATAAG TGGTCTCACC
    AATATATTAA AAAATGTTAA TAATATGAGA ATATTTGGAG AAGCTGCCGA AAAGAATTTG
    TATCTCTCTC AATTGATTAT ATTGGATACA CTGGAAAAAT GTCTTGCCGG GCAACCAAAG
    GACACCATGC GATTGGATGA GACTATGCTG GTCAAGCAGT TGCTGCCGGA AATCTGCCAT
    TTTATCCACA CCTATCGGGA AGGAAACCAA CATGCAGCTG AGCTGCGGAA TTCTGCCTCG
    GGAGTTCTCT TCTCTCTCAG CTGTAATAAC TTCAATGCAG TCTTCAGCCG TATTTCCACC
    AGGTTACAGG AGCTGACTGT GTGCTCAGAA GATAATGTTG ATGTCCATGA TATAGAATTG
    TTACAGTACA TCAATGTGGA CTGTGCAAAG CTGAAGCGAC TGCTGCAGGA AACAGTATTT
    AAGTTTAAAG CCCTTAAGAA AGTAGCTCAG CTGGCAGTTA TAAACAGTCT GGAAAAAGCA
    TTTTGGAACT GGGTAGAGAA CTATCCTGAT GAATTTACAA AGTTGTACCA GACTCCCCAG
    ACAGACATGG CTGATTGTGC CGAAAAGTTG TTTGACTTGG TGGATGGTTT TGCTGAAAGC
    ACGAAACGTA AAGCAGCAGT TTGGCCATTG CAAATCATTC TTCTTGTCCT GTGCCCAGAA
    ATAATCCAAG ATATATCCAA GGATGTGGTG GAAGAAACCA AAATAAATAA GAAGTTGTTT
    CTGGAAAATC TCAGAAAAGC ACTCGCTGGC CATGGTGGAA GTAGGCAACT GACTGAAAGT
    GCTGCTATTG CTTGTGTCAA ATTGTGCAAA GCAAGTACCT ACATCAACTG GGAAGATAAC
    TCTGTCATTT TCCTATTAGT ACAGTCCATG GTGGTAGATC TTAAGAACCT GCTGTTTAAT
    CCAACTAAAC CCTTCTCAAG AGGCAATCAG AATGCAGATG TGGATCTAAT GATTGACTGT
    TTAGTTTCTT GTTTTCGCAT AAACCCTCAC AACAACCAAC ACTTTAAGAT CTGCTTGGCC
    CAGAGTTCTC CATCCACATT TCATTACGTG CTGGTAAATT CACTCCATCG AATCATCACA
    AATTCTGCTC TGGATTGGTG GCCCAAGATT GATGCGGTGT ACTGCCATTC AGTTGAACTC
    CGCAGCATGT TCGGTGAAAC GCTTCACAAA GCAGTGCAGG GCTGTGGAGC CCATCCAGCA
    ATACGAATGG CCACGAGTCT TACATTCAAA GAAAAAATGA CAAGTCTTAA ATTTAAAGAA
    AAACCTACAG ACTTGGAAAC AAGAAGTTAT AAATATCTCC TGTTGTCCAT GGTGAAACTA
    ATCCATGCCG ATCCAAAGCT GTTGCTCTGT AATCCGAGGA AACAAGGGCC CGAAACCCAG
    GGCAGCACAG CAGAGTTAAT CACAGGACTT GTCCAGCTTG TTCCCCAGTC AAACATGCCA
    GAAATAGCCC AAGAAGCCAT GGAGGCTTTG TTGGTTCTTC ATCAATTAGA GAGTATTGAT
    TTATGGAATC CTGATGCACC TATAGAAACA TTTTGGGAGA TTAGTTCGCA AATGCTTTTT
    TACATCTGTA AGAAGCTAAC CAGTCACCAG ATGCTTAGCA GCACAGAAAT TCTCAAATGG
    CTGCGGGAAA TTTTGATCTG CAGGAATAAA TTCCTTCTTA AAAATAAGCA GTCAGATAGG
    AGTTCCTGCC ACTTCCTTTT CCTTTATGGG GTAGCATGTG ACGTCTCTTC TGGTGGGGGT
    GCTAGTCAAA TCTCTCTAGA CCATGAAGAG TTAATACGCA CCTGTACTCC GGGAGCATCC
    CTCAGGAAGG GGAAAGGAAA CTCATCCATG GACAGTACAG TCGGATGCAG CGGAACTCCA
    ATATGCCGCC AAGCTCAGAC TAAGCTAGAA GTTGCTCTCT ACATGTTCCT CTGGAGCCCC
    GACATTGAGG CCGTCCTTGT CGCCATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCTGAC
    ATTAGGTGTG GGGTAGATGA AGTTTCCGTG CATAATTTCT TGCCAAACTA CAATACCTTC
    ATGGAGTTTG CCTCCGTCAG CAATATGATG TCTACAGGAA GAGCGGCACT TCAAAAGAGG
    GTGATGGCAC TGTTGAGACG AATTGAACAT CCAACTGCCG GCAACACTGA GGCATGGGAA
    GACACTCATA CAAAATGGGA ACAAGCTACG AAGCTGATCC TTAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGTAAC CGAAAGTCTT CACAAGACCA TTGTAAAGCG GCGAATGTCT
    CACGTGAGCG GGGGAGGATC CATCGATTTG TCTGACACGG ACTCCCTGCA GGAGTGGATC
    AATATGACGG GCTTCCTTTG TGCTCTCGGT GGGGTGTGCC TGCAGCACCG TGGCAACGCG
    GGCTTGGCCA CCTACAGCCC ACCTATGGGC CCAGTCAGTG AGCGAAAGGG TTCCATGATT
    TCTATGGTAT CCACCGAAGG AAATACCGAG ACGCCCGTCA GCAAGTTCAT GGACCGGTTG
    CTCTCCCTGA TGGTGTGCAA CCACGAGAAA GTGGGACTCC AAATACGGAC GAATATCAAG
    GACCTGGTGG GTCTCGAATT AAGTCCTGCA CTTTATCCGA TGCTATTCAA CAAGTTGAAG
    AACACCATCA GCAAATTTTT TGACTCTCAA GGGCAGGTGT TGCTGAATGA TACCAATACC
    CAATTTGTAG AGCAGACCAT AGCGATAATG AAGAACTTGC TGGATAATCA CACCGAAGGC
    AGCTCGGAGC ACCTGGGGCA AGCCAGCATA GAAACAATGA TGCTCAATCT AGTCAGGTAC
    GTTCGTGTGC TTGGGAATTT GGTACACGCA ATTCAGATTA AAACGAAACT GTGCCAGTTA
    GTTGAAGTAA TGATGGAGAG GCGAGATGAT CTTTCGTTTT GCCAAGAAAT GAAATTTAGG
    AACAAGATGG TGGAATATCT GACAGATTGG GTCATGGGGA CATCGAACCA AGCAACAGAT
    GAAGATGTAA AGTGTTTAAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTTGTGTCT
    CTTCTTGCTG GACTCCCCCT GCAGCCGGAA GAAGGAGATG GTGTGGAATT GATGGAAGCC
    AAGTCCCAGT TATTTCTTAA GTATTTCACT TTGTTTATGA ACCTTCTCAA TGACTGCAGT
    GAGGTTGAAG ATGAAAACGC CCAAGCGGGA GGCAGGAAAC GTGGGATGTC TCGAAGACTG
    GCTTCCCTGA GGCACTGTAC CGTGCTTGCT ATGTCAAACT TACTCAACGC TAACGTGGAC
    AGCGGCCTTA TGCATTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAAAC GAGAGCCACG
    TTCATGGAAG TCCTCACGAA AATTCTCCAG CAGGGCACCG AGTTTGACAC GTTAGCGGAG
    ACCGTATTGG CCGATCGATT TGAGAGGCTG GTGGAATTAG TCACGATGAT GGGTGATCAA
    GGAGAGCTTC CCATAGCAAT GGCCCTGGCC AACGTGGTAC CTTGTTCACA ATGGGATGAG
    TTAGCTCGGG TTCTCGTCAC TCTCTTTGAT TCACGCCACT TACTCTACCA GCTGCTTTGG
    AACATGTTTT CTAAGGAAGT TGAGCTGGCA GATTCCATGC AGACTCTCTT CAGGGGGAAC
    AGTCTGGCCA GTAAAATAAT GACATTCTGC TTTAAGGTTT ACGGTGCTAC ATATCTGCAA
    AAGCTTTTGG AACCTTTACT GAGGACTGTC ATCACGTCGA TGGAATGGCA GCACGTCAGC
    TTTGAGGTGG ATCCTACAAG GTTAGAGCCT ACAGAGAGCC TTGATGATAA CCAACGGAGT
    CTCTTACAAA TGACTGAAAA GTTCTTCCAT GCTATCATCA ATTCCTCCTC AGAGTTTCCC
    CCTCAGCTTC GCAGTGTGTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG TTTTCCTCAG
    AACAGTATTG GTGCAGTAGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AGGCTGGGAT CTTAGATAAA AAGCCTCCGC CTAGAATTGA GAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TCCTGTTCAC AAAAGAAGAG
    CATATGAGGC CTTTTAATGA CTTTGTGAAA AGCAACTTCG ATGCAGCCCG AAGGTTTTTC
    CTCGATATAG CGTCTGATTG TCCTGCAAGC GATTCAGTAA ATCACAGCCT CTCCTTCATC
    AGTGATGGCA ATGTACTTGC TTTACATCGT TTGCTCTGGA ACAACCAAGA AAAGATCGGC
    CAATATCTTT CCAGCAACAG GGACCACAAG GCTGTTGGAC GTCGACCCTT TGACAAGATG
    GCTACACTCC TTGCGTATCT GGGCCCTCCT GAGCACAAGC CCGTAGCAGA TACCCACTGG
    TCCAGCCTGA ATCTGACCAG TTCCAAATTT GAGGAGTTCA TGACCAGACA CCAGGTACAT
    GAAAAAGAGG AATTTAAAGC ATTGAAAACA TTAAATATCT TCTATCAAGC AGGGACTTCC
    AAGGCCGGGA ATCCAGTTTT TTATTACATT GCACGGAGGT TCAAGACCGG TCAGATCAAT
    GGGGATTTGC TGATATACCA CGTCTTGCTG ACTCTCAAGC CATATTATGC CAAGCCATAT
    GAGATCGTCG TGGACCTTAC CCACGCCGGC CCCAGCAATC GCTTTAAAAC AGACTTTCTC
    TCCAAGTGGT TTGTCGTTTT CCCTGGCTTT GCGTACGAAA ATGTGTCTGC TGTTTACATC
    TACAACTGTA ATTCTTGGGT CAGGGAGTAC ACCAAATACC ACGAGAGACT GCTCACTGGC
    CTCAAAGGCA GCAAGAGACT TATTTTCATA GACTCGCCTG GAAAATTGGC TGAGCACATC
    GAGCACGACC AGCAGAAACT GCCGGCTGCC ACTTTGGCTT TAGAAGAGGA TTTGAAGGTG
    TTCCACAATG CTCTCAAACT GGCCCACAAA GACACCAAGG TTTCTATTAA AGTTGGCTCG
    ACTGCCGTTC AGGTCACATC AGCCGAACGG ACAAGGGTCT TGGGGCAGTC GGTTTTCCTG
    AACGACATCT ATTACGCCTC AGAGATCGAG GAGATCTGCC TCGTTGACGA AAACCAGTTC
    ACTTTAACCA TTGCTAACCA AGGCACTCCA CTCACCTTCA TGCACCAAGA GTGCGAAGCC
    ATCGTTCAAT CCATCATCCA CATACGGACA CGGTGGGAGC TGTCTCAACC CGACTCCATC
    CCCCAGCACA CGAAAATCCG GCCGAAGGAT GTCCCCGGAA CGCTCCTGAA TATCGCGTTG
    CTTAATCTGG GCAGCTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACCTGTA CCTTTAATTT AAAGATTGAA GGCCAGTTGC TAGAGACTTC AGGTCTGTGT
    ATCCCTGCCA ACAACACCCT TTTTATTGTC TCTATTAGTA AGACCTTGGC AGCTAATGAG
    CCACACCTCA CCCTGGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAGCTGAAGC ACCTTTGCTT AGAATATATG ACTCCCTGGC TATCAAATCT AGTGCGTTTC
    TGTAAACACA ATGATGATGC CAAACGCCAG CGAGTCACTG CTATTCTTGA CAAGCTCATA
    ACAATGACGA TTAATGAGAA ACAGATGTAT CCATCTATCC AAGCCAAGAT CTGGGGAAGT
    CTTGGACAGA TTACAGATCT GCTTGATGTC GTGCTGGACA GTTTCATCAA AACCAGTGCA
    ACTGGTGGCT TGGGCTCTAT AAAGGCTGAG GTGATGGCAG ACACAGCTGT GGCTCTTGCT
    TCCGGAAATG TGAAACTCGT TTCGAGTAAG GTCATCGGGA GAATGTGCAA AATAATCGAC
    AAGACCTGCC TGTCTCCAAC TCCCACCTTA GAACAACACC TCATGTGGGA CGATATTGCC
    ATCCTTGCCC GATACATGCT GATGCTGTCC TTTAACAACT CTCTCGACGT GGCAGCCCAC
    CTGCCCTATC TCTTCCATGT TGTTACTCTG TTGGTGGCCA CTGGTCCTCT GTCCCTTAGA
    GCTTCCACTC ACGGACTGGT CATCAACATC ATTCATTCTC TGTGCACTTG TTCACAGCTT
    CATTTCAGTG AGGAGACCAA GCAAGTTTTG AGGCTCAGCT TGACAGAGTT CTCATTACCC
    AAGTTTTATT TGCTGTTTGG CATCAGCAAA GTCAAGTCGG CAGCTGTCAT AGCCTTCCGG
    TCCAGTTACC GGGACAGGTC TTTCTCACCT GGTTCCTACG AGAGGGAGAC GTTTGCCTTG
    ACATCCCTGG AGACCGTGAC AGAGGCTCTA CTGGAGATTA TGGAGGCTTG TATGAGAGAT
    ATTCCAACGT GCAAGTGGCT GGACCAATGG ACAGAACTAG CACAAAAATT TGCATTCCAG
    TATAATCCAT CACTCCAGCC AAGAGCCCTC GTTGTCTTCG GTTGTATCAG CAAAAGGGTA
    TCTCACGGGC AGATAAAACA AATTATCCGT ATTCTTAGCA AGGGACTTGA GAGCTGTCTA
    AAGGGACCTG ATAATTACAA CAGTCAAGTT CTGATAGAAG CCACAGTAAT AGCACTGACC
    AAATTACAGC CACTTTTGAA TAAGGATTCT CCTATGCACA AAGCCCTCTT TTGGGTGGCT
    ATGGCTGTGT TGCAGCTGGA TGAAGTTAAC TTGTATTCAG CTGGGACAGC ACTTCTTGAG
    CAGAACCTGC ACACGTTAGA TAGTCTCCGG GTATTCAATG ACAAGAGTCC AGAAGAAGTC
    TTCATGGAAA TCCGAAATCC GCTTGAATGG CACTGCAAAC AAATGGATCA TTTTGTTGGA
    CTCAATTTTA ACTCTAATTT TAATTTTGCA CTAGTAGGAC ACCTTTTAAA AGGATACAGG
    CATCCTTCTC CTATCATTGT TGCAAGAACA GTAAGGATCT TACACACACT ACTAGCTCTG
    GTTAACAAAC ATAGGAACTG TGACAAATTT GAGGTGAATA CCCAGAGTGT GGCTTACTTA
    GCAGCTTTGC TTACAGTGTC TGAGGAAGTG CGAAGTCGCT GTAGCCTAAA ACATAGGAAA
    TCACTGCTGC TGACAGATGT CTCTATGGAA AATGTTCCTA TGGATACATA TCCCATCCAT
    CACAACGATC CCAGCTGTAG GACACTAAAG GAAAATCAAC CATGGTCCTC CCCAAAGGGC
    TCCGAGAGAC ATCTCGCCGC CAGCTACCCG ACTGTTGGTC AGACCAGCCC CCGAACCCGG
    AAGTCCATGA GTTTGGATAT GGGACAGCCT TCCCAGGCCA ACACTAAGAA GCTGCTTGGA
    ACCAGGAAAA GCTTTGACCA CTTGATATCA GACACAAAGG CTCCTAAAAG GCAAGAAATG
    GAATCTGGGA TCACCACCCC TCCCAAAATG AGGAGAGTGG CCGAGAATGA CTATGAAATG
    GAAACCCAGA GAATCTCCTC ACCTCAACAG CACCCACACC TGCGCAAAGT TTCTGTGTCT
    GAATCAAATG TACTTCTTGA TGAAGAAGTT CTAACCGATC CCAAAATCCA AGCCCTGCTG
    CTTACTGTCC TGGCTACACT GGTAAAATAC ACTACAGACG AGTTTGATCA ACGGATCCTT
    TATGAATATT TAGCCGAGGC CAGTGTTGTC TTCCCTAAAG TTTTTCCAGT TGTGCACAAT
    CTGTTGGACT CCAAGATTAA TACCCTTCTG TCGCTATGCC AAGATCCAAA TCTACTGAAT
    CCAATTCATG GGATCGTGCA GAGCGTGGTC TACCATGAAG AGTCGCCACC ACAGTACCAA
    ACCTCTTATC TACAAAGTTT TGGTTTTAAT GGCTTGTGGA GGTTTGCGGG ACCATTTTCT
    AAGCAAACCC AAATTCCAGA CTATGCTGAG CTGATTGTGA AATTTCTCGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA GCCAGTGAGG AGTCCTTACT GACCCCCACC
    TCTCCTTATC CTCCAGCAGT GCAGAGCCAG CTGAGCATTA CTGCTAATCT TAACCTTTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GCATTGACAA GGAGAATGTG
    GAGCTCTCTC CGACCACTGG CCACTCCAAC AGTGGGAGGA CACGCCACGG CTCCGCAAGC
    CAAGTGCAAA AGCAGCGAAG TGCCGGCAGT TTCAAACGTC ACAGTATCAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc119300
    Clone ID Related Accession (Same CDS sequence) XM_029082637.1
    Accession Version XM_029082637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8460bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041744.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCCGCGC ACAAGCCGGT GGAGTGGGTC CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AAGCCGGACA GCAGAGCACG CACACCAAAG TCAGTACTGA ACACAACAAG
    GAGTGTCTTA TCAACATTTC TAAATACAAG TTTTCTTTGG TTATAAGTGG TCTCACCAAT
    ATATTAAAAA ATGTTAATAA TATGAGAATA TTTGGAGAAG CTGCCGAAAA GAATTTGTAT
    CTCTCTCAAT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC
    ACCATGCGAT TGGATGAGAC TATGCTGGTC AAGCAGTTGC TGCCGGAAAT CTGCCATTTT
    ATCCACACCT ATCGGGAAGG AAACCAACAT GCAGCTGAGC TGCGGAATTC TGCCTCGGGA
    GTTCTCTTCT CTCTCAGCTG TAATAACTTC AATGCAGTCT TCAGCCGTAT TTCCACCAGG
    TTACAGGAGC TGACTGTGTG CTCAGAAGAT AATGTTGATG TCCATGATAT AGAATTGTTA
    CAGTACATCA ATGTGGACTG TGCAAAGCTG AAGCGACTGC TGCAGGAAAC AGTATTTAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGCTG GCAGTTATAA ACAGTCTGGA AAAAGCATTT
    TGGAACTGGG TAGAGAACTA TCCTGATGAA TTTACAAAGT TGTACCAGAC TCCCCAGACA
    GACATGGCTG ATTGTGCCGA AAAGTTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACG
    AAACGTAAAG CAGCAGTTTG GCCATTGCAA ATCATTCTTC TTGTCCTGTG CCCAGAAATA
    ATCCAAGATA TATCCAAGGA TGTGGTGGAA GAAACCAAAA TAAATAAGAA GTTGTTTCTG
    GAAAATCTCA GAAAAGCACT CGCTGGCCAT GGTGGAAGTA GGCAACTGAC TGAAAGTGCT
    GCTATTGCTT GTGTCAAATT GTGCAAAGCA AGTACCTACA TCAACTGGGA AGATAACTCT
    GTCATTTTCC TATTAGTACA GTCCATGGTG GTAGATCTTA AGAACCTGCT GTTTAATCCA
    ACTAAACCCT TCTCAAGAGG CAATCAGAAT GCAGATGTGG ATCTAATGAT TGACTGTTTA
    GTTTCTTGTT TTCGCATAAA CCCTCACAAC AACCAACACT TTAAGATCTG CTTGGCCCAG
    AGTTCTCCAT CCACATTTCA TTACGTGCTG GTAAATTCAC TCCATCGAAT CATCACAAAT
    TCTGCTCTGG ATTGGTGGCC CAAGATTGAT GCGGTGTACT GCCATTCAGT TGAACTCCGC
    AGCATGTTCG GTGAAACGCT TCACAAAGCA GTGCAGGGCT GTGGAGCCCA TCCAGCAATA
    CGAATGGCCA CGAGTCTTAC ATTCAAAGAA AAAATGACAA GTCTTAAATT TAAAGAAAAA
    CCTACAGACT TGGAAACAAG AAGTTATAAA TATCTCCTGT TGTCCATGGT GAAACTAATC
    CATGCCGATC CAAAGCTGTT GCTCTGTAAT CCGAGGAAAC AAGGGCCCGA AACCCAGGGC
    AGCACAGCAG AGTTAATCAC AGGACTTGTC CAGCTTGTTC CCCAGTCAAA CATGCCAGAA
    ATAGCCCAAG AAGCCATGGA GGCTTTGTTG GTTCTTCATC AATTAGAGAG TATTGATTTA
    TGGAATCCTG ATGCACCTAT AGAAACATTT TGGGAGATTA GTTCGCAAAT GCTTTTTTAC
    ATCTGTAAGA AGCTAACCAG TCACCAGATG CTTAGCAGCA CAGAAATTCT CAAATGGCTG
    CGGGAAATTT TGATCTGCAG GAATAAATTC CTTCTTAAAA ATAAGCAGTC AGATAGGAGT
    TCCTGCCACT TCCTTTTCCT TTATGGGGTA GCATGTGACG TCTCTTCTGG TGGGGGTGCT
    AGTCAAATCT CTCTAGACCA TGAAGAGTTA ATACGCACCT GTACTCCGGG AGCATCCCTC
    AGGAAGGGGA AAGGAAACTC ATCCATGGAC AGTACAGTCG GATGCAGCGG AACTCCAATA
    TGCCGCCAAG CTCAGACTAA GCTAGAAGTT GCTCTCTACA TGTTCCTCTG GAGCCCCGAC
    ATTGAGGCCG TCCTTGTCGC CATGTCCTGT TTCCGCCACC TCTGTGAAGA AGCTGACATT
    AGGTGTGGGG TAGATGAAGT TTCCGTGCAT AATTTCTTGC CAAACTACAA TACCTTCATG
    GAGTTTGCCT CCGTCAGCAA TATGATGTCT ACAGGAAGAG CGGCACTTCA AAAGAGGGTG
    ATGGCACTGT TGAGACGAAT TGAACATCCA ACTGCCGGCA ACACTGAGGC ATGGGAAGAC
    ACTCATACAA AATGGGAACA AGCTACGAAG CTGATCCTTA ACTATCCAAA AGCCAAAATG
    GAAGATGGCC AGGTAACCGA AAGTCTTCAC AAGACCATTG TAAAGCGGCG AATGTCTCAC
    GTGAGCGGGG GAGGATCCAT CGATTTGTCT GACACGGACT CCCTGCAGGA GTGGATCAAT
    ATGACGGGCT TCCTTTGTGC TCTCGGTGGG GTGTGCCTGC AGCACCGTGG CAACGCGGGC
    TTGGCCACCT ACAGCCCACC TATGGGCCCA GTCAGTGAGC GAAAGGGTTC CATGATTTCT
    ATGGTATCCA CCGAAGGAAA TACCGAGACG CCCGTCAGCA AGTTCATGGA CCGGTTGCTC
    TCCCTGATGG TGTGCAACCA CGAGAAAGTG GGACTCCAAA TACGGACGAA TATCAAGGAC
    CTGGTGGGTC TCGAATTAAG TCCTGCACTT TATCCGATGC TATTCAACAA GTTGAAGAAC
    ACCATCAGCA AATTTTTTGA CTCTCAAGGG CAGGTGTTGC TGAATGATAC CAATACCCAA
    TTTGTAGAGC AGACCATAGC GATAATGAAG AACTTGCTGG ATAATCACAC CGAAGGCAGC
    TCGGAGCACC TGGGGCAAGC CAGCATAGAA ACAATGATGC TCAATCTAGT CAGGTACGTT
    CGTGTGCTTG GGAATTTGGT ACACGCAATT CAGATTAAAA CGAAACTGTG CCAGTTAGTT
    GAAGTAATGA TGGAGAGGCG AGATGATCTT TCGTTTTGCC AAGAAATGAA ATTTAGGAAC
    AAGATGGTGG AATATCTGAC AGATTGGGTC ATGGGGACAT CGAACCAAGC AACAGATGAA
    GATGTAAAGT GTTTAACAAG AGATTTGGAC CAGGCAAGCA TGGAAGCAGT TGTGTCTCTT
    CTTGCTGGAC TCCCCCTGCA GCCGGAAGAA GGAGATGGTG TGGAATTGAT GGAAGCCAAG
    TCCCAGTTAT TTCTTAAGTA TTTCACTTTG TTTATGAACC TTCTCAATGA CTGCAGTGAG
    GTTGAAGATG AAAACGCCCA AGCGGGAGGC AGGAAACGTG GGATGTCTCG AAGACTGGCT
    TCCCTGAGGC ACTGTACCGT GCTTGCTATG TCAAACTTAC TCAACGCTAA CGTGGACAGC
    GGCCTTATGC ATTCCATAGG TTTAGGTTAT CACAAAGATC TCCAAACGAG AGCCACGTTC
    ATGGAAGTCC TCACGAAAAT TCTCCAGCAG GGCACCGAGT TTGACACGTT AGCGGAGACC
    GTATTGGCCG ATCGATTTGA GAGGCTGGTG GAATTAGTCA CGATGATGGG TGATCAAGGA
    GAGCTTCCCA TAGCAATGGC CCTGGCCAAC GTGGTACCTT GTTCACAATG GGATGAGTTA
    GCTCGGGTTC TCGTCACTCT CTTTGATTCA CGCCACTTAC TCTACCAGCT GCTTTGGAAC
    ATGTTTTCTA AGGAAGTTGA GCTGGCAGAT TCCATGCAGA CTCTCTTCAG GGGGAACAGT
    CTGGCCAGTA AAATAATGAC ATTCTGCTTT AAGGTTTACG GTGCTACATA TCTGCAAAAG
    CTTTTGGAAC CTTTACTGAG GACTGTCATC ACGTCGATGG AATGGCAGCA CGTCAGCTTT
    GAGGTGGATC CTACAAGGTT AGAGCCTACA GAGAGCCTTG ATGATAACCA ACGGAGTCTC
    TTACAAATGA CTGAAAAGTT CTTCCATGCT ATCATCAATT CCTCCTCAGA GTTTCCCCCT
    CAGCTTCGCA GTGTGTGCCA TTGTTTGTAC CAGGTGGTTA GCCAGCGTTT TCCTCAGAAC
    AGTATTGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTCA TCAATCCTGC CATTGTCTCG
    CCGTATGAGG CTGGGATCTT AGATAAAAAG CCTCCGCCTA GAATTGAGAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTCC TGTTCACAAA AGAAGAGCAT
    ATGAGGCCTT TTAATGACTT TGTGAAAAGC AACTTCGATG CAGCCCGAAG GTTTTTCCTC
    GATATAGCGT CTGATTGTCC TGCAAGCGAT TCAGTAAATC ACAGCCTCTC CTTCATCAGT
    GATGGCAATG TACTTGCTTT ACATCGTTTG CTCTGGAACA ACCAAGAAAA GATCGGCCAA
    TATCTTTCCA GCAACAGGGA CCACAAGGCT GTTGGACGTC GACCCTTTGA CAAGATGGCT
    ACACTCCTTG CGTATCTGGG CCCTCCTGAG CACAAGCCCG TAGCAGATAC CCACTGGTCC
    AGCCTGAATC TGACCAGTTC CAAATTTGAG GAGTTCATGA CCAGACACCA GGTACATGAA
    AAAGAGGAAT TTAAAGCATT GAAAACATTA AATATCTTCT ATCAAGCAGG GACTTCCAAG
    GCCGGGAATC CAGTTTTTTA TTACATTGCA CGGAGGTTCA AGACCGGTCA GATCAATGGG
    GATTTGCTGA TATACCACGT CTTGCTGACT CTCAAGCCAT ATTATGCCAA GCCATATGAG
    ATCGTCGTGG ACCTTACCCA CGCCGGCCCC AGCAATCGCT TTAAAACAGA CTTTCTCTCC
    AAGTGGTTTG TCGTTTTCCC TGGCTTTGCG TACGAAAATG TGTCTGCTGT TTACATCTAC
    AACTGTAATT CTTGGGTCAG GGAGTACACC AAATACCACG AGAGACTGCT CACTGGCCTC
    AAAGGCAGCA AGAGACTTAT TTTCATAGAC TCGCCTGGAA AATTGGCTGA GCACATCGAG
    CACGACCAGC AGAAACTGCC GGCTGCCACT TTGGCTTTAG AAGAGGATTT GAAGGTGTTC
    CACAATGCTC TCAAACTGGC CCACAAAGAC ACCAAGGTTT CTATTAAAGT TGGCTCGACT
    GCCGTTCAGG TCACATCAGC CGAACGGACA AGGGTCTTGG GGCAGTCGGT TTTCCTGAAC
    GACATCTATT ACGCCTCAGA GATCGAGGAG ATCTGCCTCG TTGACGAAAA CCAGTTCACT
    TTAACCATTG CTAACCAAGG CACTCCACTC ACCTTCATGC ACCAAGAGTG CGAAGCCATC
    GTTCAATCCA TCATCCACAT ACGGACACGG TGGGAGCTGT CTCAACCCGA CTCCATCCCC
    CAGCACACGA AAATCCGGCC GAAGGATGTC CCCGGAACGC TCCTGAATAT CGCGTTGCTT
    AATCTGGGCA GCTCAGACCC TAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACCTGTACCT TTAATTTAAA GATTGAAGGC CAGTTGCTAG AGACTTCAGG TCTGTGTATC
    CCTGCCAACA ACACCCTTTT TATTGTCTCT ATTAGTAAGA CCTTGGCAGC TAATGAGCCA
    CACCTCACCC TGGAGTTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAG
    CTGAAGCACC TTTGCTTAGA ATATATGACT CCCTGGCTAT CAAATCTAGT GCGTTTCTGT
    AAACACAATG ATGATGCCAA ACGCCAGCGA GTCACTGCTA TTCTTGACAA GCTCATAACA
    ATGACGATTA ATGAGAAACA GATGTATCCA TCTATCCAAG CCAAGATCTG GGGAAGTCTT
    GGACAGATTA CAGATCTGCT TGATGTCGTG CTGGACAGTT TCATCAAAAC CAGTGCAACT
    GGTGGCTTGG GCTCTATAAA GGCTGAGGTG ATGGCAGACA CAGCTGTGGC TCTTGCTTCC
    GGAAATGTGA AACTCGTTTC GAGTAAGGTC ATCGGGAGAA TGTGCAAAAT AATCGACAAG
    ACCTGCCTGT CTCCAACTCC CACCTTAGAA CAACACCTCA TGTGGGACGA TATTGCCATC
    CTTGCCCGAT ACATGCTGAT GCTGTCCTTT AACAACTCTC TCGACGTGGC AGCCCACCTG
    CCCTATCTCT TCCATGTTGT TACTCTGTTG GTGGCCACTG GTCCTCTGTC CCTTAGAGCT
    TCCACTCACG GACTGGTCAT CAACATCATT CATTCTCTGT GCACTTGTTC ACAGCTTCAT
    TTCAGTGAGG AGACCAAGCA AGTTTTGAGG CTCAGCTTGA CAGAGTTCTC ATTACCCAAG
    TTTTATTTGC TGTTTGGCAT CAGCAAAGTC AAGTCGGCAG CTGTCATAGC CTTCCGGTCC
    AGTTACCGGG ACAGGTCTTT CTCACCTGGT TCCTACGAGA GGGAGACGTT TGCCTTGACA
    TCCCTGGAGA CCGTGACAGA GGCTCTACTG GAGATTATGG AGGCTTGTAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAATGGACA GAACTAGCAC AAAAATTTGC ATTCCAGTAT
    AATCCATCAC TCCAGCCAAG AGCCCTCGTT GTCTTCGGTT GTATCAGCAA AAGGGTATCT
    CACGGGCAGA TAAAACAAAT TATCCGTATT CTTAGCAAGG GACTTGAGAG CTGTCTAAAG
    GGACCTGATA ATTACAACAG TCAAGTTCTG ATAGAAGCCA CAGTAATAGC ACTGACCAAA
    TTACAGCCAC TTTTGAATAA GGATTCTCCT ATGCACAAAG CCCTCTTTTG GGTGGCTATG
    GCTGTGTTGC AGCTGGATGA AGTTAACTTG TATTCAGCTG GGACAGCACT TCTTGAGCAG
    AACCTGCACA CGTTAGATAG TCTCCGGGTA TTCAATGACA AGAGTCCAGA AGAAGTCTTC
    ATGGAAATCC GAAATCCGCT TGAATGGCAC TGCAAACAAA TGGATCATTT TGTTGGACTC
    AATTTTAACT CTAATTTTAA TTTTGCACTA GTAGGACACC TTTTAAAAGG ATACAGGCAT
    CCTTCTCCTA TCATTGTTGC AAGAACAGTA AGGATCTTAC ACACACTACT AGCTCTGGTT
    AACAAACATA GGAACTGTGA CAAATTTGAG GTGAATACCC AGAGTGTGGC TTACTTAGCA
    GCTTTGCTTA CAGTGTCTGA GGAAGTGCGA AGTCGCTGTA GCCTAAAACA TAGGAAATCA
    CTGCTGCTGA CAGATGTCTC TATGGAAAAT GTTCCTATGG ATACATATCC CATCCATCAC
    AACGATCCCA GCTGTAGGAC ACTAAAGGAA AATCAACCAT GGTCCTCCCC AAAGGGCTCC
    GAGAGACATC TCGCCGCCAG CTACCCGACT GTTGGTCAGA CCAGCCCCCG AACCCGGAAG
    TCCATGAGTT TGGATATGGG ACAGCCTTCC CAGGCCAACA CTAAGAAGCT GCTTGGAACC
    AGGAAAAGCT TTGACCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCTGGGATCA CCACCCCTCC CAAAATGAGG AGAGTGGCCG AGAATGACTA TGAAATGGAA
    ACCCAGAGAA TCTCCTCACC TCAACAGCAC CCACACCTGC GCAAAGTTTC TGTGTCTGAA
    TCAAATGTAC TTCTTGATGA AGAAGTTCTA ACCGATCCCA AAATCCAAGC CCTGCTGCTT
    ACTGTCCTGG CTACACTGGT AAAATACACT ACAGACGAGT TTGATCAACG GATCCTTTAT
    GAATATTTAG CCGAGGCCAG TGTTGTCTTC CCTAAAGTTT TTCCAGTTGT GCACAATCTG
    TTGGACTCCA AGATTAATAC CCTTCTGTCG CTATGCCAAG ATCCAAATCT ACTGAATCCA
    ATTCATGGGA TCGTGCAGAG CGTGGTCTAC CATGAAGAGT CGCCACCACA GTACCAAACC
    TCTTATCTAC AAAGTTTTGG TTTTAATGGC TTGTGGAGGT TTGCGGGACC ATTTTCTAAG
    CAAACCCAAA TTCCAGACTA TGCTGAGCTG ATTGTGAAAT TTCTCGATGC CTTGATTGAC
    ACGTATTTGC CTGGAATTGA TGAAGAAGCC AGTGAGGAGT CCTTACTGAC CCCCACCTCT
    CCTTATCCTC CAGCAGTGCA GAGCCAGCTG AGCATTACTG CTAATCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGCA TTGACAAGGA GAATGTGGAG
    CTCTCTCCGA CCACTGGCCA CTCCAACAGT GGGAGGACAC GCCACGGCTC CGCAAGCCAA
    GTGCAAAAGC AGCGAAGTGC CGGCAGTTTC AAACGTCACA GTATCAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028938470.1
    CDS42..8501
    Translation

    Target ORF information:

    RefSeq Version XM_029082637.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus neurofibromin 1 (NF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029082637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCCGCGC ACAAGCCGGT GGAGTGGGTC CAGGCCGTGG TGAACCGCTT CGACGAGCAG 
    CTTCCAATAA AAGCCGGACA GCAGAGCACG CACACCAAAG TCAGTACTGA ACACAACAAG
    GAGTGTCTTA TCAACATTTC TAAATACAAG TTTTCTTTGG TTATAAGTGG TCTCACCAAT
    ATATTAAAAA ATGTTAATAA TATGAGAATA TTTGGAGAAG CTGCCGAAAA GAATTTGTAT
    CTCTCTCAAT TGATTATATT GGATACACTG GAAAAATGTC TTGCCGGGCA ACCAAAGGAC
    ACCATGCGAT TGGATGAGAC TATGCTGGTC AAGCAGTTGC TGCCGGAAAT CTGCCATTTT
    ATCCACACCT ATCGGGAAGG AAACCAACAT GCAGCTGAGC TGCGGAATTC TGCCTCGGGA
    GTTCTCTTCT CTCTCAGCTG TAATAACTTC AATGCAGTCT TCAGCCGTAT TTCCACCAGG
    TTACAGGAGC TGACTGTGTG CTCAGAAGAT AATGTTGATG TCCATGATAT AGAATTGTTA
    CAGTACATCA ATGTGGACTG TGCAAAGCTG AAGCGACTGC TGCAGGAAAC AGTATTTAAG
    TTTAAAGCCC TTAAGAAAGT AGCTCAGCTG GCAGTTATAA ACAGTCTGGA AAAAGCATTT
    TGGAACTGGG TAGAGAACTA TCCTGATGAA TTTACAAAGT TGTACCAGAC TCCCCAGACA
    GACATGGCTG ATTGTGCCGA AAAGTTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACG
    AAACGTAAAG CAGCAGTTTG GCCATTGCAA ATCATTCTTC TTGTCCTGTG CCCAGAAATA
    ATCCAAGATA TATCCAAGGA TGTGGTGGAA GAAACCAAAA TAAATAAGAA GTTGTTTCTG
    GAAAATCTCA GAAAAGCACT CGCTGGCCAT GGTGGAAGTA GGCAACTGAC TGAAAGTGCT
    GCTATTGCTT GTGTCAAATT GTGCAAAGCA AGTACCTACA TCAACTGGGA AGATAACTCT
    GTCATTTTCC TATTAGTACA GTCCATGGTG GTAGATCTTA AGAACCTGCT GTTTAATCCA
    ACTAAACCCT TCTCAAGAGG CAATCAGAAT GCAGATGTGG ATCTAATGAT TGACTGTTTA
    GTTTCTTGTT TTCGCATAAA CCCTCACAAC AACCAACACT TTAAGATCTG CTTGGCCCAG
    AGTTCTCCAT CCACATTTCA TTACGTGCTG GTAAATTCAC TCCATCGAAT CATCACAAAT
    TCTGCTCTGG ATTGGTGGCC CAAGATTGAT GCGGTGTACT GCCATTCAGT TGAACTCCGC
    AGCATGTTCG GTGAAACGCT TCACAAAGCA GTGCAGGGCT GTGGAGCCCA TCCAGCAATA
    CGAATGGCCA CGAGTCTTAC ATTCAAAGAA AAAATGACAA GTCTTAAATT TAAAGAAAAA
    CCTACAGACT TGGAAACAAG AAGTTATAAA TATCTCCTGT TGTCCATGGT GAAACTAATC
    CATGCCGATC CAAAGCTGTT GCTCTGTAAT CCGAGGAAAC AAGGGCCCGA AACCCAGGGC
    AGCACAGCAG AGTTAATCAC AGGACTTGTC CAGCTTGTTC CCCAGTCAAA CATGCCAGAA
    ATAGCCCAAG AAGCCATGGA GGCTTTGTTG GTTCTTCATC AATTAGAGAG TATTGATTTA
    TGGAATCCTG ATGCACCTAT AGAAACATTT TGGGAGATTA GTTCGCAAAT GCTTTTTTAC
    ATCTGTAAGA AGCTAACCAG TCACCAGATG CTTAGCAGCA CAGAAATTCT CAAATGGCTG
    CGGGAAATTT TGATCTGCAG GAATAAATTC CTTCTTAAAA ATAAGCAGTC AGATAGGAGT
    TCCTGCCACT TCCTTTTCCT TTATGGGGTA GCATGTGACG TCTCTTCTGG TGGGGGTGCT
    AGTCAAATCT CTCTAGACCA TGAAGAGTTA ATACGCACCT GTACTCCGGG AGCATCCCTC
    AGGAAGGGGA AAGGAAACTC ATCCATGGAC AGTACAGTCG GATGCAGCGG AACTCCAATA
    TGCCGCCAAG CTCAGACTAA GCTAGAAGTT GCTCTCTACA TGTTCCTCTG GAGCCCCGAC
    ATTGAGGCCG TCCTTGTCGC CATGTCCTGT TTCCGCCACC TCTGTGAAGA AGCTGACATT
    AGGTGTGGGG TAGATGAAGT TTCCGTGCAT AATTTCTTGC CAAACTACAA TACCTTCATG
    GAGTTTGCCT CCGTCAGCAA TATGATGTCT ACAGGAAGAG CGGCACTTCA AAAGAGGGTG
    ATGGCACTGT TGAGACGAAT TGAACATCCA ACTGCCGGCA ACACTGAGGC ATGGGAAGAC
    ACTCATACAA AATGGGAACA AGCTACGAAG CTGATCCTTA ACTATCCAAA AGCCAAAATG
    GAAGATGGCC AGGTAACCGA AAGTCTTCAC AAGACCATTG TAAAGCGGCG AATGTCTCAC
    GTGAGCGGGG GAGGATCCAT CGATTTGTCT GACACGGACT CCCTGCAGGA GTGGATCAAT
    ATGACGGGCT TCCTTTGTGC TCTCGGTGGG GTGTGCCTGC AGCACCGTGG CAACGCGGGC
    TTGGCCACCT ACAGCCCACC TATGGGCCCA GTCAGTGAGC GAAAGGGTTC CATGATTTCT
    ATGGTATCCA CCGAAGGAAA TACCGAGACG CCCGTCAGCA AGTTCATGGA CCGGTTGCTC
    TCCCTGATGG TGTGCAACCA CGAGAAAGTG GGACTCCAAA TACGGACGAA TATCAAGGAC
    CTGGTGGGTC TCGAATTAAG TCCTGCACTT TATCCGATGC TATTCAACAA GTTGAAGAAC
    ACCATCAGCA AATTTTTTGA CTCTCAAGGG CAGGTGTTGC TGAATGATAC CAATACCCAA
    TTTGTAGAGC AGACCATAGC GATAATGAAG AACTTGCTGG ATAATCACAC CGAAGGCAGC
    TCGGAGCACC TGGGGCAAGC CAGCATAGAA ACAATGATGC TCAATCTAGT CAGGTACGTT
    CGTGTGCTTG GGAATTTGGT ACACGCAATT CAGATTAAAA CGAAACTGTG CCAGTTAGTT
    GAAGTAATGA TGGAGAGGCG AGATGATCTT TCGTTTTGCC AAGAAATGAA ATTTAGGAAC
    AAGATGGTGG AATATCTGAC AGATTGGGTC ATGGGGACAT CGAACCAAGC AACAGATGAA
    GATGTAAAGT GTTTAACAAG AGATTTGGAC CAGGCAAGCA TGGAAGCAGT TGTGTCTCTT
    CTTGCTGGAC TCCCCCTGCA GCCGGAAGAA GGAGATGGTG TGGAATTGAT GGAAGCCAAG
    TCCCAGTTAT TTCTTAAGTA TTTCACTTTG TTTATGAACC TTCTCAATGA CTGCAGTGAG
    GTTGAAGATG AAAACGCCCA AGCGGGAGGC AGGAAACGTG GGATGTCTCG AAGACTGGCT
    TCCCTGAGGC ACTGTACCGT GCTTGCTATG TCAAACTTAC TCAACGCTAA CGTGGACAGC
    GGCCTTATGC ATTCCATAGG TTTAGGTTAT CACAAAGATC TCCAAACGAG AGCCACGTTC
    ATGGAAGTCC TCACGAAAAT TCTCCAGCAG GGCACCGAGT TTGACACGTT AGCGGAGACC
    GTATTGGCCG ATCGATTTGA GAGGCTGGTG GAATTAGTCA CGATGATGGG TGATCAAGGA
    GAGCTTCCCA TAGCAATGGC CCTGGCCAAC GTGGTACCTT GTTCACAATG GGATGAGTTA
    GCTCGGGTTC TCGTCACTCT CTTTGATTCA CGCCACTTAC TCTACCAGCT GCTTTGGAAC
    ATGTTTTCTA AGGAAGTTGA GCTGGCAGAT TCCATGCAGA CTCTCTTCAG GGGGAACAGT
    CTGGCCAGTA AAATAATGAC ATTCTGCTTT AAGGTTTACG GTGCTACATA TCTGCAAAAG
    CTTTTGGAAC CTTTACTGAG GACTGTCATC ACGTCGATGG AATGGCAGCA CGTCAGCTTT
    GAGGTGGATC CTACAAGGTT AGAGCCTACA GAGAGCCTTG ATGATAACCA ACGGAGTCTC
    TTACAAATGA CTGAAAAGTT CTTCCATGCT ATCATCAATT CCTCCTCAGA GTTTCCCCCT
    CAGCTTCGCA GTGTGTGCCA TTGTTTGTAC CAGGTGGTTA GCCAGCGTTT TCCTCAGAAC
    AGTATTGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTCA TCAATCCTGC CATTGTCTCG
    CCGTATGAGG CTGGGATCTT AGATAAAAAG CCTCCGCCTA GAATTGAGAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTCC TGTTCACAAA AGAAGAGCAT
    ATGAGGCCTT TTAATGACTT TGTGAAAAGC AACTTCGATG CAGCCCGAAG GTTTTTCCTC
    GATATAGCGT CTGATTGTCC TGCAAGCGAT TCAGTAAATC ACAGCCTCTC CTTCATCAGT
    GATGGCAATG TACTTGCTTT ACATCGTTTG CTCTGGAACA ACCAAGAAAA GATCGGCCAA
    TATCTTTCCA GCAACAGGGA CCACAAGGCT GTTGGACGTC GACCCTTTGA CAAGATGGCT
    ACACTCCTTG CGTATCTGGG CCCTCCTGAG CACAAGCCCG TAGCAGATAC CCACTGGTCC
    AGCCTGAATC TGACCAGTTC CAAATTTGAG GAGTTCATGA CCAGACACCA GGTACATGAA
    AAAGAGGAAT TTAAAGCATT GAAAACATTA AATATCTTCT ATCAAGCAGG GACTTCCAAG
    GCCGGGAATC CAGTTTTTTA TTACATTGCA CGGAGGTTCA AGACCGGTCA GATCAATGGG
    GATTTGCTGA TATACCACGT CTTGCTGACT CTCAAGCCAT ATTATGCCAA GCCATATGAG
    ATCGTCGTGG ACCTTACCCA CGCCGGCCCC AGCAATCGCT TTAAAACAGA CTTTCTCTCC
    AAGTGGTTTG TCGTTTTCCC TGGCTTTGCG TACGAAAATG TGTCTGCTGT TTACATCTAC
    AACTGTAATT CTTGGGTCAG GGAGTACACC AAATACCACG AGAGACTGCT CACTGGCCTC
    AAAGGCAGCA AGAGACTTAT TTTCATAGAC TCGCCTGGAA AATTGGCTGA GCACATCGAG
    CACGACCAGC AGAAACTGCC GGCTGCCACT TTGGCTTTAG AAGAGGATTT GAAGGTGTTC
    CACAATGCTC TCAAACTGGC CCACAAAGAC ACCAAGGTTT CTATTAAAGT TGGCTCGACT
    GCCGTTCAGG TCACATCAGC CGAACGGACA AGGGTCTTGG GGCAGTCGGT TTTCCTGAAC
    GACATCTATT ACGCCTCAGA GATCGAGGAG ATCTGCCTCG TTGACGAAAA CCAGTTCACT
    TTAACCATTG CTAACCAAGG CACTCCACTC ACCTTCATGC ACCAAGAGTG CGAAGCCATC
    GTTCAATCCA TCATCCACAT ACGGACACGG TGGGAGCTGT CTCAACCCGA CTCCATCCCC
    CAGCACACGA AAATCCGGCC GAAGGATGTC CCCGGAACGC TCCTGAATAT CGCGTTGCTT
    AATCTGGGCA GCTCAGACCC TAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACCTGTACCT TTAATTTAAA GATTGAAGGC CAGTTGCTAG AGACTTCAGG TCTGTGTATC
    CCTGCCAACA ACACCCTTTT TATTGTCTCT ATTAGTAAGA CCTTGGCAGC TAATGAGCCA
    CACCTCACCC TGGAGTTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAG
    CTGAAGCACC TTTGCTTAGA ATATATGACT CCCTGGCTAT CAAATCTAGT GCGTTTCTGT
    AAACACAATG ATGATGCCAA ACGCCAGCGA GTCACTGCTA TTCTTGACAA GCTCATAACA
    ATGACGATTA ATGAGAAACA GATGTATCCA TCTATCCAAG CCAAGATCTG GGGAAGTCTT
    GGACAGATTA CAGATCTGCT TGATGTCGTG CTGGACAGTT TCATCAAAAC CAGTGCAACT
    GGTGGCTTGG GCTCTATAAA GGCTGAGGTG ATGGCAGACA CAGCTGTGGC TCTTGCTTCC
    GGAAATGTGA AACTCGTTTC GAGTAAGGTC ATCGGGAGAA TGTGCAAAAT AATCGACAAG
    ACCTGCCTGT CTCCAACTCC CACCTTAGAA CAACACCTCA TGTGGGACGA TATTGCCATC
    CTTGCCCGAT ACATGCTGAT GCTGTCCTTT AACAACTCTC TCGACGTGGC AGCCCACCTG
    CCCTATCTCT TCCATGTTGT TACTCTGTTG GTGGCCACTG GTCCTCTGTC CCTTAGAGCT
    TCCACTCACG GACTGGTCAT CAACATCATT CATTCTCTGT GCACTTGTTC ACAGCTTCAT
    TTCAGTGAGG AGACCAAGCA AGTTTTGAGG CTCAGCTTGA CAGAGTTCTC ATTACCCAAG
    TTTTATTTGC TGTTTGGCAT CAGCAAAGTC AAGTCGGCAG CTGTCATAGC CTTCCGGTCC
    AGTTACCGGG ACAGGTCTTT CTCACCTGGT TCCTACGAGA GGGAGACGTT TGCCTTGACA
    TCCCTGGAGA CCGTGACAGA GGCTCTACTG GAGATTATGG AGGCTTGTAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAATGGACA GAACTAGCAC AAAAATTTGC ATTCCAGTAT
    AATCCATCAC TCCAGCCAAG AGCCCTCGTT GTCTTCGGTT GTATCAGCAA AAGGGTATCT
    CACGGGCAGA TAAAACAAAT TATCCGTATT CTTAGCAAGG GACTTGAGAG CTGTCTAAAG
    GGACCTGATA ATTACAACAG TCAAGTTCTG ATAGAAGCCA CAGTAATAGC ACTGACCAAA
    TTACAGCCAC TTTTGAATAA GGATTCTCCT ATGCACAAAG CCCTCTTTTG GGTGGCTATG
    GCTGTGTTGC AGCTGGATGA AGTTAACTTG TATTCAGCTG GGACAGCACT TCTTGAGCAG
    AACCTGCACA CGTTAGATAG TCTCCGGGTA TTCAATGACA AGAGTCCAGA AGAAGTCTTC
    ATGGAAATCC GAAATCCGCT TGAATGGCAC TGCAAACAAA TGGATCATTT TGTTGGACTC
    AATTTTAACT CTAATTTTAA TTTTGCACTA GTAGGACACC TTTTAAAAGG ATACAGGCAT
    CCTTCTCCTA TCATTGTTGC AAGAACAGTA AGGATCTTAC ACACACTACT AGCTCTGGTT
    AACAAACATA GGAACTGTGA CAAATTTGAG GTGAATACCC AGAGTGTGGC TTACTTAGCA
    GCTTTGCTTA CAGTGTCTGA GGAAGTGCGA AGTCGCTGTA GCCTAAAACA TAGGAAATCA
    CTGCTGCTGA CAGATGTCTC TATGGAAAAT GTTCCTATGG ATACATATCC CATCCATCAC
    AACGATCCCA GCTGTAGGAC ACTAAAGGAA AATCAACCAT GGTCCTCCCC AAAGGGCTCC
    GAGAGACATC TCGCCGCCAG CTACCCGACT GTTGGTCAGA CCAGCCCCCG AACCCGGAAG
    TCCATGAGTT TGGATATGGG ACAGCCTTCC CAGGCCAACA CTAAGAAGCT GCTTGGAACC
    AGGAAAAGCT TTGACCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCTGGGATCA CCACCCCTCC CAAAATGAGG AGAGTGGCCG AGAATGACTA TGAAATGGAA
    ACCCAGAGAA TCTCCTCACC TCAACAGCAC CCACACCTGC GCAAAGTTTC TGTGTCTGAA
    TCAAATGTAC TTCTTGATGA AGAAGTTCTA ACCGATCCCA AAATCCAAGC CCTGCTGCTT
    ACTGTCCTGG CTACACTGGT AAAATACACT ACAGACGAGT TTGATCAACG GATCCTTTAT
    GAATATTTAG CCGAGGCCAG TGTTGTCTTC CCTAAAGTTT TTCCAGTTGT GCACAATCTG
    TTGGACTCCA AGATTAATAC CCTTCTGTCG CTATGCCAAG ATCCAAATCT ACTGAATCCA
    ATTCATGGGA TCGTGCAGAG CGTGGTCTAC CATGAAGAGT CGCCACCACA GTACCAAACC
    TCTTATCTAC AAAGTTTTGG TTTTAATGGC TTGTGGAGGT TTGCGGGACC ATTTTCTAAG
    CAAACCCAAA TTCCAGACTA TGCTGAGCTG ATTGTGAAAT TTCTCGATGC CTTGATTGAC
    ACGTATTTGC CTGGAATTGA TGAAGAAGCC AGTGAGGAGT CCTTACTGAC CCCCACCTCT
    CCTTATCCTC CAGCAGTGCA GAGCCAGCTG AGCATTACTG CTAATCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGCA TTGACAAGGA GAATGTGGAG
    CTCTCTCCGA CCACTGGCCA CTCCAACAGT GGGAGGACAC GCCACGGCTC CGCAAGCCAA
    GTGCAAAAGC AGCGAAGTGC CGGCAGTTTC AAACGTCACA GTATCAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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