NEDD4L cDNA ORF clone, Ornithorhynchus anatinus(platypus)

  • Gene

  • Clones

  • gRNAs

The following NEDD4L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOc00892 XM_007659798.1
Latest version!
Ornithorhynchus anatinus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OOc125297 XM_029059769.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OOc125298 XM_029059770.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OOc125299 XM_029059771.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OOc125287 XM_029059758.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OOc125288 XM_029059759.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125289 XM_029059760.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125290 XM_029059761.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125291 XM_029059762.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125292 XM_029059763.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125293 XM_029059765.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125294 XM_029059766.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125295 XM_029059767.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125296 XM_029059768.1
Latest version!
Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOc00892
    Clone ID Related Accession (Same CDS sequence) XM_007659798.1
    Accession Version XM_007659798.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-04-14
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG 
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TTAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGACCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AATAGTAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCA GCTGTTCCCT ATTCCAGAGA GTTTAAACAG
    AAATATGACT ATTTTAGGAA GAAGTTAAAG AAACCAGCTG ATATTCCAAA TAGATTTGAA
    ATGAAGCTCC ACAGAAATAA CATATTTGAA GAATCATACA GGAGGATCAT GTCAGTGAAG
    AGACCTGATG TACTAAAAGC CAGGCTATGG ATTGAATTTG AATCTGAAAA AGGACTAGAT
    TATGGGGGCG TGGCCAGAGA ATGGTTCTTC CTATTGTCTA AGGAAATGTT CAATCCTTAC
    TACGGTCTCT TTGAGTACTC CGCAACGGAC AACTACACAC TTCAAATCAA TCCCAATTCA
    GGCCTTTGTA ATGAAGATCA TCTGTCCTAT TTCACTTTTA TTGGAAGGGT TGCTGGGCTG
    GCAGTGTTTC ATGGGAAGCT CTTAGATGGA TTCTTCATTA GACCCTTTTA CAAAATGATG
    TTGGGGAAGC AAATAACATT GAATGACATG GAGTCAGTGG ACAGTGAATA TTATAACTCT
    TTGAAATGGA TCTTGGAAAA TGATCCTACT GAACTGGATC TTATGTTTTG CATAGATGAA
    GAAAATTTTG GACAGACATA CCAAGTGGAT TTGAAACCCA ACGGATCTGA AATTATGGTA
    ACAAATGAAA ATAAAAGGGA ATATATTGAC CTAGTCATCC AGTGGAGATT TGTAAACCGA
    GTTCAGAAAC AAATGAATGC CTTCTTGGAG GGATTCACAG AACTGCTTCC TACAGATTTG
    ATTAAAATTT TTGATGAGAA TGAGCTGGAG TTGCTCATGT GTGGCCTTGG AGATGTTGAT
    GTTAATGACT GGAGACAACA TGCTATTTAC AAGAATGGCT ATTGCCCAAA TCACCCAGTC
    ATTCAGTGGT TCTGGAAGAT TATATTATTG AAACTACAGC ATTGCTACTA CCAGGATCAG
    AGTTGGCAGA AGCTCAAAAC CATTCCATAT GGAAATATGT TCTGCCTCCC AACTCTCAAT
    CCCCAAGACG GTGCCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007657988.1
    CDS64..2604
    Translation

    Target ORF information:

    RefSeq Version XM_007659798.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_007659798.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG 
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TTAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGACCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AATAGTAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCA GCTGTTCCCT ATTCCAGAGA GTTTAAACAG
    AAATATGACT ATTTTAGGAA GAAGTTAAAG AAACCAGCTG ATATTCCAAA TAGATTTGAA
    ATGAAGCTCC ACAGAAATAA CATATTTGAA GAATCATACA GGAGGATCAT GTCAGTGAAG
    AGACCTGATG TACTAAAAGC CAGGCTATGG ATTGAATTTG AATCTGAAAA AGGACTAGAT
    TATGGGGGCG TGGCCAGAGA ATGGTTCTTC CTATTGTCTA AGGAAATGTT CAATCCTTAC
    TACGGTCTCT TTGAGTACTC CGCAACGGAC AACTACACAC TTCAAATCAA TCCCAATTCA
    GGCCTTTGTA ATGAAGATCA TCTGTCCTAT TTCACTTTTA TTGGAAGGGT TGCTGGGCTG
    GCAGTGTTTC ATGGGAAGCT CTTAGATGGA TTCTTCATTA GACCCTTTTA CAAAATGATG
    TTGGGGAAGC AAATAACATT GAATGACATG GAGTCAGTGG ACAGTGAATA TTATAACTCT
    TTGAAATGGA TCTTGGAAAA TGATCCTACT GAACTGGATC TTATGTTTTG CATAGATGAA
    GAAAATTTTG GACAGACATA CCAAGTGGAT TTGAAACCCA ACGGATCTGA AATTATGGTA
    ACAAATGAAA ATAAAAGGGA ATATATTGAC CTAGTCATCC AGTGGAGATT TGTAAACCGA
    GTTCAGAAAC AAATGAATGC CTTCTTGGAG GGATTCACAG AACTGCTTCC TACAGATTTG
    ATTAAAATTT TTGATGAGAA TGAGCTGGAG TTGCTCATGT GTGGCCTTGG AGATGTTGAT
    GTTAATGACT GGAGACAACA TGCTATTTAC AAGAATGGCT ATTGCCCAAA TCACCCAGTC
    ATTCAGTGGT TCTGGAAGAT TATATTATTG AAACTACAGC ATTGCTACTA CCAGGATCAG
    AGTTGGCAGA AGCTCAAAAC CATTCCATAT GGAAATATGT TCTGCCTCCC AACTCTCAAT
    CCCCAAGACG GTGCCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125297
    Clone ID Related Accession (Same CDS sequence) XM_029059769.1
    Accession Version XM_029059769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTGGGAGA GCCGGTGTAT GGACTTTCTG AAGACGAGGG GGAGTCGCGC 
    ATCCTCAGAG TAAAAGTTGT TTCTGGAATT GACCTGGCGA AGAAGGACAT CTTTGGAGCC
    AGTGACCCGT ATGTGAAACT TTCATTGTAT GTAGCTGATG AGAATCGAGA ACTTGCTTTG
    GTACAGACCA AAACCATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTCATTTT
    AGAGTGAACC CAACTAATCA TAGGCTCTTG TTTGAAGTGT TTGATGAAAA CAGATTGACG
    AGAGACGACT TCTTGGGGCA GGTGGACGTG CCTCTTAATC ATCTACCGAC AGAAGACCCA
    ACCATGGAAC GACCCTACAC CTTTAAGGAT TTTCTCCTTA GGCCAAGGAG TCACAAATCC
    CGGGTAAAGG GCTTCTTGAG ATTGAAGATG GCCTACATGC CGAAGAATGG AGGACAAGAT
    GAAGAAAATA ATGAACAAAG GGAAGAGTTG GAGCACGGGT GGGAGGTCGT TGACTCCAAT
    GACTCAGCTT CCCAACACCA GGAGGAATTG CCCCCACCAC CTCTTCCCCC CGGATGGGAA
    GAGAAAGTTG ATAACTTGGG GCGAACTTAT TACGTCAACC ACAACAACAG GACTACCCAG
    TGGCACCGAC CAAGTTTAAT TGACGTGTCT TCTGAATCGG ATAATAATAT CAGGCAGATA
    AATCAGGAAG CAGCCCACAG ACGATTCCGC TCTCGTAGAC ACATCAGTGA AGATTTAGAA
    CCTGAACCTG CAGAAGGTGG AGATGCTCCT GAGCCCTGGG AAACCATCTC AGAGGAAGTG
    AACATAGCCA GTGACTCTAT GAGTCTGGCA TTGCCACCAC CTCCTGCTTC TCCAGTCTCT
    CGGACCAGTC CTCAAGAGCT GTCAGAGGAA TTGAACAGAA GACTTCAGAT CACTCCGGAT
    TCTAATGGGG AACAATTCAG TTCTTTAATT CAAAGGGAAC CTCCTTCAAG ACTGAGGTCA
    TGCAGTGTAA CAGATACGGT GGCTGAACAG GCCCATCTAC CCCTGCAGAG TGTCCCAACT
    AGGAGAGCTC GTTCTTCAAC TGTCACAGGT GGTGAGGAGC CAACGCCATC AGTGGCCTAC
    GTACATACTA CGCCGGGACT ACCTTCAGGC TGGGAAGAAC GGAAAGATGC TAAGGGACGC
    ACGTACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCTGAAGATG GTGCTACCGG GTCAGCCGCA AACAGTAACA ACCACCTAAG TGAACCACAG
    ATACGTCGGC CTCGTAGCCT CAGTTCACCC ACAGTAACTT TGTCTGCCCC CCTGGAGGGT
    GCCAAAGATT CTCCCGTTCG CCGAGCTGTG AAGGACACCC TTTCCAATCC ACAGTCCCCA
    CAGCCTTCAC CTTACAATTC TCCAAAGCCT CAGCACAAAG TGACACAAAG CTTCTTGCCA
    CCTGGTTGGG AGATGCGGAT TGCTCCAAAT GGCCGGCCCT TCTTCATTGA TCATAATACA
    AAGACGACCA CATGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGCG ATCAAAAGCA
    TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAAGAAAG AATTCACTTG
    GATGGACGAA CGTTTTATAT AGATCATAAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915602.1
    CDS108..3035
    Translation

    Target ORF information:

    RefSeq Version XM_029059769.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACCG GGCTGGGAGA GCCGGTGTAT GGACTTTCTG AAGACGAGGG GGAGTCGCGC 
    ATCCTCAGAG TAAAAGTTGT TTCTGGAATT GACCTGGCGA AGAAGGACAT CTTTGGAGCC
    AGTGACCCGT ATGTGAAACT TTCATTGTAT GTAGCTGATG AGAATCGAGA ACTTGCTTTG
    GTACAGACCA AAACCATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTCATTTT
    AGAGTGAACC CAACTAATCA TAGGCTCTTG TTTGAAGTGT TTGATGAAAA CAGATTGACG
    AGAGACGACT TCTTGGGGCA GGTGGACGTG CCTCTTAATC ATCTACCGAC AGAAGACCCA
    ACCATGGAAC GACCCTACAC CTTTAAGGAT TTTCTCCTTA GGCCAAGGAG TCACAAATCC
    CGGGTAAAGG GCTTCTTGAG ATTGAAGATG GCCTACATGC CGAAGAATGG AGGACAAGAT
    GAAGAAAATA ATGAACAAAG GGAAGAGTTG GAGCACGGGT GGGAGGTCGT TGACTCCAAT
    GACTCAGCTT CCCAACACCA GGAGGAATTG CCCCCACCAC CTCTTCCCCC CGGATGGGAA
    GAGAAAGTTG ATAACTTGGG GCGAACTTAT TACGTCAACC ACAACAACAG GACTACCCAG
    TGGCACCGAC CAAGTTTAAT TGACGTGTCT TCTGAATCGG ATAATAATAT CAGGCAGATA
    AATCAGGAAG CAGCCCACAG ACGATTCCGC TCTCGTAGAC ACATCAGTGA AGATTTAGAA
    CCTGAACCTG CAGAAGGTGG AGATGCTCCT GAGCCCTGGG AAACCATCTC AGAGGAAGTG
    AACATAGCCA GTGACTCTAT GAGTCTGGCA TTGCCACCAC CTCCTGCTTC TCCAGTCTCT
    CGGACCAGTC CTCAAGAGCT GTCAGAGGAA TTGAACAGAA GACTTCAGAT CACTCCGGAT
    TCTAATGGGG AACAATTCAG TTCTTTAATT CAAAGGGAAC CTCCTTCAAG ACTGAGGTCA
    TGCAGTGTAA CAGATACGGT GGCTGAACAG GCCCATCTAC CCCTGCAGAG TGTCCCAACT
    AGGAGAGCTC GTTCTTCAAC TGTCACAGGT GGTGAGGAGC CAACGCCATC AGTGGCCTAC
    GTACATACTA CGCCGGGACT ACCTTCAGGC TGGGAAGAAC GGAAAGATGC TAAGGGACGC
    ACGTACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCTGAAGATG GTGCTACCGG GTCAGCCGCA AACAGTAACA ACCACCTAAG TGAACCACAG
    ATACGTCGGC CTCGTAGCCT CAGTTCACCC ACAGTAACTT TGTCTGCCCC CCTGGAGGGT
    GCCAAAGATT CTCCCGTTCG CCGAGCTGTG AAGGACACCC TTTCCAATCC ACAGTCCCCA
    CAGCCTTCAC CTTACAATTC TCCAAAGCCT CAGCACAAAG TGACACAAAG CTTCTTGCCA
    CCTGGTTGGG AGATGCGGAT TGCTCCAAAT GGCCGGCCCT TCTTCATTGA TCATAATACA
    AAGACGACCA CATGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGCG ATCAAAAGCA
    TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAAGAAAG AATTCACTTG
    GATGGACGAA CGTTTTATAT AGATCATAAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125298
    Clone ID Related Accession (Same CDS sequence) XM_029059770.1
    Accession Version XM_029059770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTGGGAGA GCCGGTGTAT GGACTTTCTG AAGACGAGGG GGAGTCGCGC 
    ATCCTCAGAG TAAAAGTTGT TTCTGGAATT GACCTGGCGA AGAAGGACAT CTTTGGAGCC
    AGTGACCCGT ATGTGAAACT TTCATTGTAT GTAGCTGATG AGAATCGAGA ACTTGCTTTG
    GTACAGACCA AAACCATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTCATTTT
    AGAGTGAACC CAACTAATCA TAGGCTCTTG TTTGAAGTGT TTGATGAAAA CAGATTGACG
    AGAGACGACT TCTTGGGGCA GGTGGACGTG CCTCTTAATC ATCTACCGAC AGAAGACCCA
    ACCATGGAAC GACCCTACAC CTTTAAGGAT TTTCTCCTTA GGCCAAGGAG TCACAAATCC
    CGGGTAAAGG GCTTCTTGAG ATTGAAGATG GCCTACATGC CGAAGAATGG AGGACAAGAT
    GAAGAAAATA ATGAACAAAG GGAAGAGTTG GAGCACGGGT GGGAGGTCGT TGACTCCAAT
    GACTCAGCTT CCCAACACCA GGAGGAATTG CCCCCACCAC CTCTTCCCCC CGGATGGGAA
    GAGAAAGTTG ATAACTTGGG GCGAACTTAT TACGTCAACC ACAACAACAG GACTACCCAG
    TGGCACCGAC CAAGTTTAAT TGACGTGTCT TCTGAATCGG ATAATAATAT CAGGCAGATA
    AATCAGGAAG CAGCCCACAG ACGATTCCGC TCTCGTAGAC ACATCAGTGA AGATTTAGAA
    CCTGAACCTG CAGAAGGTGG AGATGCTCCT GAGCCCTGGG AAACCATCTC AGAGGAAGTG
    AACATAGCCA GTGACTCTAT GAGTCTGGCA TTGCCACCAC CTCCTGCTTC TCCAGTCTCT
    CGGACCAGTC CTCAAGAGCT GTCAGAGGAA TTGAACAGAA GACTTCAGAT CACTCCGGAT
    TCTAATGGGG AACAATTCAG TTCTTTAATT CAAAGGGAAC CTCCTTCAAG ACTGAGGTCA
    TGCAGTGTAA CAGATACGGT GGCTGAACAG GCCCATCTAC CCCTGCCATC AGTGGCCTAC
    GTACATACTA CGCCGGGACT ACCTTCAGGC TGGGAAGAAC GGAAAGATGC TAAGGGACGC
    ACGTACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCTGAAGATG GTGCTACCGG GTCAGCCGCA AACAGTAACA ACCACCTAAG TGAACCACAG
    ATACGTCGGC CTCGTAGCCT CAGTTCACCC ACAGTAACTT TGTCTGCCCC CCTGGAGGGT
    GCCAAAGATT CTCCCGTTCG CCGAGCTGTG AAGGACACCC TTTCCAATCC ACAGTCCCCA
    CAGCCTTCAC CTTACAATTC TCCAAAGCCT CAGCACAAAG TGACACAAAG CTTCTTGCCA
    CCTGGTTGGG AGATGCGGAT TGCTCCAAAT GGCCGGCCCT TCTTCATTGA TCATAATACA
    AAGACGACCA CATGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGCG ATCAAAAGCA
    TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAAGAAAG AATTCACTTG
    GATGGACGAA CGTTTTATAT AGATCATAAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915603.1
    CDS107..2974
    Translation

    Target ORF information:

    RefSeq Version XM_029059770.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACCG GGCTGGGAGA GCCGGTGTAT GGACTTTCTG AAGACGAGGG GGAGTCGCGC 
    ATCCTCAGAG TAAAAGTTGT TTCTGGAATT GACCTGGCGA AGAAGGACAT CTTTGGAGCC
    AGTGACCCGT ATGTGAAACT TTCATTGTAT GTAGCTGATG AGAATCGAGA ACTTGCTTTG
    GTACAGACCA AAACCATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTCATTTT
    AGAGTGAACC CAACTAATCA TAGGCTCTTG TTTGAAGTGT TTGATGAAAA CAGATTGACG
    AGAGACGACT TCTTGGGGCA GGTGGACGTG CCTCTTAATC ATCTACCGAC AGAAGACCCA
    ACCATGGAAC GACCCTACAC CTTTAAGGAT TTTCTCCTTA GGCCAAGGAG TCACAAATCC
    CGGGTAAAGG GCTTCTTGAG ATTGAAGATG GCCTACATGC CGAAGAATGG AGGACAAGAT
    GAAGAAAATA ATGAACAAAG GGAAGAGTTG GAGCACGGGT GGGAGGTCGT TGACTCCAAT
    GACTCAGCTT CCCAACACCA GGAGGAATTG CCCCCACCAC CTCTTCCCCC CGGATGGGAA
    GAGAAAGTTG ATAACTTGGG GCGAACTTAT TACGTCAACC ACAACAACAG GACTACCCAG
    TGGCACCGAC CAAGTTTAAT TGACGTGTCT TCTGAATCGG ATAATAATAT CAGGCAGATA
    AATCAGGAAG CAGCCCACAG ACGATTCCGC TCTCGTAGAC ACATCAGTGA AGATTTAGAA
    CCTGAACCTG CAGAAGGTGG AGATGCTCCT GAGCCCTGGG AAACCATCTC AGAGGAAGTG
    AACATAGCCA GTGACTCTAT GAGTCTGGCA TTGCCACCAC CTCCTGCTTC TCCAGTCTCT
    CGGACCAGTC CTCAAGAGCT GTCAGAGGAA TTGAACAGAA GACTTCAGAT CACTCCGGAT
    TCTAATGGGG AACAATTCAG TTCTTTAATT CAAAGGGAAC CTCCTTCAAG ACTGAGGTCA
    TGCAGTGTAA CAGATACGGT GGCTGAACAG GCCCATCTAC CCCTGCCATC AGTGGCCTAC
    GTACATACTA CGCCGGGACT ACCTTCAGGC TGGGAAGAAC GGAAAGATGC TAAGGGACGC
    ACGTACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCTGAAGATG GTGCTACCGG GTCAGCCGCA AACAGTAACA ACCACCTAAG TGAACCACAG
    ATACGTCGGC CTCGTAGCCT CAGTTCACCC ACAGTAACTT TGTCTGCCCC CCTGGAGGGT
    GCCAAAGATT CTCCCGTTCG CCGAGCTGTG AAGGACACCC TTTCCAATCC ACAGTCCCCA
    CAGCCTTCAC CTTACAATTC TCCAAAGCCT CAGCACAAAG TGACACAAAG CTTCTTGCCA
    CCTGGTTGGG AGATGCGGAT TGCTCCAAAT GGCCGGCCCT TCTTCATTGA TCATAATACA
    AAGACGACCA CATGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGCG ATCAAAAGCA
    TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAAGAAAG AATTCACTTG
    GATGGACGAA CGTTTTATAT AGATCATAAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125299
    Clone ID Related Accession (Same CDS sequence) XM_029059771.1
    Accession Version XM_029059771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG 
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AGAAGCCAGG TGTTGATATG TGGAGACATT
    GATACCAGAG ACATAGTGCC TTCCTACGAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915604.1
    CDS315..3062
    Translation

    Target ORF information:

    RefSeq Version XM_029059771.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG 
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AGAAGCCAGG TGTTGATATG TGGAGACATT
    GATACCAGAG ACATAGTGCC TTCCTACGAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125287
    Clone ID Related Accession (Same CDS sequence) XM_029059758.1
    Accession Version XM_029059758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AGAAGCCAGG TGTTGATATG TGGAGACATT
    GATACCAGAG ACATAGTGCC TTCCTACGAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915591.1
    CDS34..4041
    Translation

    Target ORF information:

    RefSeq Version XM_029059758.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AGAAGCCAGG TGTTGATATG TGGAGACATT
    GATACCAGAG ACATAGTGCC TTCCTACGAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125288
    Clone ID Related Accession (Same CDS sequence) XM_029059759.1
    Accession Version XM_029059759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AATAGTAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCA GCTGTTCCCT ATTCCAGAGA GTTTAAACAG
    AAATATGACT ATTTTAGGAA GAAGTTAAAG AAACCAGCTG ATATTCCAAA TAGATTTGAA
    ATGAAGCTCC ACAGAAATAA CATATTTGAA GAATCATACA GGAGGATCAT GTCAGTGAAG
    AGACCTGATG TACTAAAAGC CAGGCTATGG ATTGAATTTG AATCTGAAAA AGGACTAGAT
    TATGGGGGCG TGGCCAGAGA ATGGTTCTTC CTATTGTCTA AGGAAATGTT CAATCCTTAC
    TACGGTCTCT TTGAGTACTC CGCAACGGAC AACTACACAC TTCAAATCAA TCCCAATTCA
    GGCCTTTGTA ATGAAGATCA TCTGTCCTAT TTCACTTTTA TTGGAAGGGT TGCTGGGCTG
    GCAGTGTTTC ATGGGAAGCT CTTAGATGGA TTCTTCATTA GACCCTTTTA CAAAATGATG
    TTGGGGAAGC AAATAACATT GAATGACATG GAGTCAGTGG ACAGTGAATA TTATAACTCT
    TTGAAATGGA TCTTGGAAAA TGATCCTACT GAACTGGATC TTATGTTTTG CATAGATGAA
    GAAAATTTTG GACAGACATA CCAAGTGGAT TTGAAACCCA ACGGATCTGA AATTATGGTA
    ACAAATGAAA ATAAAAGGGA ATATATTGAC CTAGTCATCC AGTGGAGATT TGTAAACCGA
    GTTCAGAAAC AAATGAATGC CTTCTTGGAG GGATTCACAG AACTGCTTCC TACAGATTTG
    ATTAAAATTT TTGATGAGAA TGAGCTGGAG TTGCTCATGT GTGGCCTTGG AGATGTTGAT
    GTTAATGACT GGAGACAACA TGCTATTTAC AAGAATGGCT ATTGCCCAAA TCACCCAGTC
    ATTCAGTGGT TCTGGAAGGC TGTGTTATTG ATGGATGCTG AGAAGCGTAT CCGGTTACTT
    CAGTTTGTTA CCGGGACCTC CCGGGTACCT ATGAATGGAT TTGCTGAGCT TTATGGTTCA
    AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT
    CACACATGCT TTAATCGCCT CGATTTACCT CCATATGAAT CTTTCGAAGA TTTAAGAGAG
    AAACTTCTCA TGGCCGTTGA AAACGCCCAA GGATTTGAAG GGGTGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915592.1
    CDS32..3982
    Translation

    Target ORF information:

    RefSeq Version XM_029059759.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AATAGTAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCA GCTGTTCCCT ATTCCAGAGA GTTTAAACAG
    AAATATGACT ATTTTAGGAA GAAGTTAAAG AAACCAGCTG ATATTCCAAA TAGATTTGAA
    ATGAAGCTCC ACAGAAATAA CATATTTGAA GAATCATACA GGAGGATCAT GTCAGTGAAG
    AGACCTGATG TACTAAAAGC CAGGCTATGG ATTGAATTTG AATCTGAAAA AGGACTAGAT
    TATGGGGGCG TGGCCAGAGA ATGGTTCTTC CTATTGTCTA AGGAAATGTT CAATCCTTAC
    TACGGTCTCT TTGAGTACTC CGCAACGGAC AACTACACAC TTCAAATCAA TCCCAATTCA
    GGCCTTTGTA ATGAAGATCA TCTGTCCTAT TTCACTTTTA TTGGAAGGGT TGCTGGGCTG
    GCAGTGTTTC ATGGGAAGCT CTTAGATGGA TTCTTCATTA GACCCTTTTA CAAAATGATG
    TTGGGGAAGC AAATAACATT GAATGACATG GAGTCAGTGG ACAGTGAATA TTATAACTCT
    TTGAAATGGA TCTTGGAAAA TGATCCTACT GAACTGGATC TTATGTTTTG CATAGATGAA
    GAAAATTTTG GACAGACATA CCAAGTGGAT TTGAAACCCA ACGGATCTGA AATTATGGTA
    ACAAATGAAA ATAAAAGGGA ATATATTGAC CTAGTCATCC AGTGGAGATT TGTAAACCGA
    GTTCAGAAAC AAATGAATGC CTTCTTGGAG GGATTCACAG AACTGCTTCC TACAGATTTG
    ATTAAAATTT TTGATGAGAA TGAGCTGGAG TTGCTCATGT GTGGCCTTGG AGATGTTGAT
    GTTAATGACT GGAGACAACA TGCTATTTAC AAGAATGGCT ATTGCCCAAA TCACCCAGTC
    ATTCAGTGGT TCTGGAAGGC TGTGTTATTG ATGGATGCTG AGAAGCGTAT CCGGTTACTT
    CAGTTTGTTA CCGGGACCTC CCGGGTACCT ATGAATGGAT TTGCTGAGCT TTATGGTTCA
    AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT
    CACACATGCT TTAATCGCCT CGATTTACCT CCATATGAAT CTTTCGAAGA TTTAAGAGAG
    AAACTTCTCA TGGCCGTTGA AAACGCCCAA GGATTTGAAG GGGTGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125289
    Clone ID Related Accession (Same CDS sequence) XM_029059760.1
    Accession Version XM_029059760.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AGAAGCCAGG TGTTGATATG TGGAGACATT
    GATACCAGAG ACATAGTGCC TTCCTACGAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915593.1
    CDS32..3979
    Translation

    Target ORF information:

    RefSeq Version XM_029059760.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGCTGATACC CAGCAGAGTA CTGATCACAC GGAGCTGTAC TGATGTTGAA
    GTTACTGGTG AAGAGGCAGA GAATGGGGGC AACCAGCCAT GCTCTCTGCT CAGTTCGGTT
    AGAAACACAG CATCAAAAGA AGATCCACGA CTGAAATTTC CAGTACATTT GCGATCAAAA
    GCATCTTTGA ACCCTAATGA TTTGGGCCCT CTTCCTCCTG GTTGGGAAGA AAGAATTCAC
    TTGGATGGAC GAACGTTTTA TATAGATCAT AGAAGCCAGG TGTTGATATG TGGAGACATT
    GATACCAGAG ACATAGTGCC TTCCTACGAT AGTAAAATTA CTCAGTGGGA AGACCCAAGA
    CTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT TAAACAGAAA
    TATGACTATT TTAGGAAGAA GTTAAAGAAA CCAGCTGATA TTCCAAATAG ATTTGAAATG
    AAGCTCCACA GAAATAACAT ATTTGAAGAA TCATACAGGA GGATCATGTC AGTGAAGAGA
    CCTGATGTAC TAAAAGCCAG GCTATGGATT GAATTTGAAT CTGAAAAAGG ACTAGATTAT
    GGGGGCGTGG CCAGAGAATG GTTCTTCCTA TTGTCTAAGG AAATGTTCAA TCCTTACTAC
    GGTCTCTTTG AGTACTCCGC AACGGACAAC TACACACTTC AAATCAATCC CAATTCAGGC
    CTTTGTAATG AAGATCATCT GTCCTATTTC ACTTTTATTG GAAGGGTTGC TGGGCTGGCA
    GTGTTTCATG GGAAGCTCTT AGATGGATTC TTCATTAGAC CCTTTTACAA AATGATGTTG
    GGGAAGCAAA TAACATTGAA TGACATGGAG TCAGTGGACA GTGAATATTA TAACTCTTTG
    AAATGGATCT TGGAAAATGA TCCTACTGAA CTGGATCTTA TGTTTTGCAT AGATGAAGAA
    AATTTTGGAC AGACATACCA AGTGGATTTG AAACCCAACG GATCTGAAAT TATGGTAACA
    AATGAAAATA AAAGGGAATA TATTGACCTA GTCATCCAGT GGAGATTTGT AAACCGAGTT
    CAGAAACAAA TGAATGCCTT CTTGGAGGGA TTCACAGAAC TGCTTCCTAC AGATTTGATT
    AAAATTTTTG ATGAGAATGA GCTGGAGTTG CTCATGTGTG GCCTTGGAGA TGTTGATGTT
    AATGACTGGA GACAACATGC TATTTACAAG AATGGCTATT GCCCAAATCA CCCAGTCATT
    CAGTGGTTCT GGAAGGCTGT GTTATTGATG GATGCTGAGA AGCGTATCCG GTTACTTCAG
    TTTGTTACCG GGACCTCCCG GGTACCTATG AATGGATTTG CTGAGCTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCCTCGA TTTACCTCCA TATGAATCTT TCGAAGATTT AAGAGAGAAA
    CTTCTCATGG CCGTTGAAAA CGCCCAAGGA TTTGAAGGGG TGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125290
    Clone ID Related Accession (Same CDS sequence) XM_029059761.1
    Accession Version XM_029059761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGGAAGATCC ACGACTGAAA TTTCCAGTAC ATTTGCGATC AAAAGCATCT
    TTGAACCCTA ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGACGAACGT TTTATATAGA TCATAGAAGC CAGGTGTTGA TATGTGGAGA CATTGATACC
    AGAGACATAG TGCCTTCCTA CGATAGTAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC AGCTGTTCCC TATTCCAGAG AGTTTAAACA GAAATATGAC
    TATTTTAGGA AGAAGTTAAA GAAACCAGCT GATATTCCAA ATAGATTTGA AATGAAGCTC
    CACAGAAATA ACATATTTGA AGAATCATAC AGGAGGATCA TGTCAGTGAA GAGACCTGAT
    GTACTAAAAG CCAGGCTATG GATTGAATTT GAATCTGAAA AAGGACTAGA TTATGGGGGC
    GTGGCCAGAG AATGGTTCTT CCTATTGTCT AAGGAAATGT TCAATCCTTA CTACGGTCTC
    TTTGAGTACT CCGCAACGGA CAACTACACA CTTCAAATCA ATCCCAATTC AGGCCTTTGT
    AATGAAGATC ATCTGTCCTA TTTCACTTTT ATTGGAAGGG TTGCTGGGCT GGCAGTGTTT
    CATGGGAAGC TCTTAGATGG ATTCTTCATT AGACCCTTTT ACAAAATGAT GTTGGGGAAG
    CAAATAACAT TGAATGACAT GGAGTCAGTG GACAGTGAAT ATTATAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAT CTTATGTTTT GCATAGATGA AGAAAATTTT
    GGACAGACAT ACCAAGTGGA TTTGAAACCC AACGGATCTG AAATTATGGT AACAAATGAA
    AATAAAAGGG AATATATTGA CCTAGTCATC CAGTGGAGAT TTGTAAACCG AGTTCAGAAA
    CAAATGAATG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTACAGATTT GATTAAAATT
    TTTGATGAGA ATGAGCTGGA GTTGCTCATG TGTGGCCTTG GAGATGTTGA TGTTAATGAC
    TGGAGACAAC ATGCTATTTA CAAGAATGGC TATTGCCCAA ATCACCCAGT CATTCAGTGG
    TTCTGGAAGG CTGTGTTATT GATGGATGCT GAGAAGCGTA TCCGGTTACT TCAGTTTGTT
    ACCGGGACCT CCCGGGTACC TATGAATGGA TTTGCTGAGC TTTATGGTTC AAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGAAGT CCTGAAAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TCGATTTACC TCCATATGAA TCTTTCGAAG ATTTAAGAGA GAAACTTCTC
    ATGGCCGTTG AAAACGCCCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915594.1
    CDS33..3914
    Translation

    Target ORF information:

    RefSeq Version XM_029059761.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGGAAGATCC ACGACTGAAA TTTCCAGTAC ATTTGCGATC AAAAGCATCT
    TTGAACCCTA ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGACGAACGT TTTATATAGA TCATAGAAGC CAGGTGTTGA TATGTGGAGA CATTGATACC
    AGAGACATAG TGCCTTCCTA CGATAGTAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG
    AACCCAGCTA TTACTGGTCC AGCTGTTCCC TATTCCAGAG AGTTTAAACA GAAATATGAC
    TATTTTAGGA AGAAGTTAAA GAAACCAGCT GATATTCCAA ATAGATTTGA AATGAAGCTC
    CACAGAAATA ACATATTTGA AGAATCATAC AGGAGGATCA TGTCAGTGAA GAGACCTGAT
    GTACTAAAAG CCAGGCTATG GATTGAATTT GAATCTGAAA AAGGACTAGA TTATGGGGGC
    GTGGCCAGAG AATGGTTCTT CCTATTGTCT AAGGAAATGT TCAATCCTTA CTACGGTCTC
    TTTGAGTACT CCGCAACGGA CAACTACACA CTTCAAATCA ATCCCAATTC AGGCCTTTGT
    AATGAAGATC ATCTGTCCTA TTTCACTTTT ATTGGAAGGG TTGCTGGGCT GGCAGTGTTT
    CATGGGAAGC TCTTAGATGG ATTCTTCATT AGACCCTTTT ACAAAATGAT GTTGGGGAAG
    CAAATAACAT TGAATGACAT GGAGTCAGTG GACAGTGAAT ATTATAACTC TTTGAAATGG
    ATCTTGGAAA ATGATCCTAC TGAACTGGAT CTTATGTTTT GCATAGATGA AGAAAATTTT
    GGACAGACAT ACCAAGTGGA TTTGAAACCC AACGGATCTG AAATTATGGT AACAAATGAA
    AATAAAAGGG AATATATTGA CCTAGTCATC CAGTGGAGAT TTGTAAACCG AGTTCAGAAA
    CAAATGAATG CCTTCTTGGA GGGATTCACA GAACTGCTTC CTACAGATTT GATTAAAATT
    TTTGATGAGA ATGAGCTGGA GTTGCTCATG TGTGGCCTTG GAGATGTTGA TGTTAATGAC
    TGGAGACAAC ATGCTATTTA CAAGAATGGC TATTGCCCAA ATCACCCAGT CATTCAGTGG
    TTCTGGAAGG CTGTGTTATT GATGGATGCT GAGAAGCGTA TCCGGTTACT TCAGTTTGTT
    ACCGGGACCT CCCGGGTACC TATGAATGGA TTTGCTGAGC TTTATGGTTC AAATGGTCCT
    CAGCTGTTTA CAATAGAGCA ATGGGGAAGT CCTGAAAAAC TGCCCAGAGC TCACACATGC
    TTTAATCGCC TCGATTTACC TCCATATGAA TCTTTCGAAG ATTTAAGAGA GAAACTTCTC
    ATGGCCGTTG AAAACGCCCA AGGATTTGAA GGGGTGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125291
    Clone ID Related Accession (Same CDS sequence) XM_029059762.1
    Accession Version XM_029059762.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGGAAGATCC ACGACTGAAA TTTCCAGTAC ATTTGCGATC AAAAGCATCT
    TTGAACCCTA ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGACGAACGT TTTATATAGA TCATAATAGT AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTTAA ACAGAAATAT
    GACTATTTTA GGAAGAAGTT AAAGAAACCA GCTGATATTC CAAATAGATT TGAAATGAAG
    CTCCACAGAA ATAACATATT TGAAGAATCA TACAGGAGGA TCATGTCAGT GAAGAGACCT
    GATGTACTAA AAGCCAGGCT ATGGATTGAA TTTGAATCTG AAAAAGGACT AGATTATGGG
    GGCGTGGCCA GAGAATGGTT CTTCCTATTG TCTAAGGAAA TGTTCAATCC TTACTACGGT
    CTCTTTGAGT ACTCCGCAAC GGACAACTAC ACACTTCAAA TCAATCCCAA TTCAGGCCTT
    TGTAATGAAG ATCATCTGTC CTATTTCACT TTTATTGGAA GGGTTGCTGG GCTGGCAGTG
    TTTCATGGGA AGCTCTTAGA TGGATTCTTC ATTAGACCCT TTTACAAAAT GATGTTGGGG
    AAGCAAATAA CATTGAATGA CATGGAGTCA GTGGACAGTG AATATTATAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GATCTTATGT TTTGCATAGA TGAAGAAAAT
    TTTGGACAGA CATACCAAGT GGATTTGAAA CCCAACGGAT CTGAAATTAT GGTAACAAAT
    GAAAATAAAA GGGAATATAT TGACCTAGTC ATCCAGTGGA GATTTGTAAA CCGAGTTCAG
    AAACAAATGA ATGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTACAGA TTTGATTAAA
    ATTTTTGATG AGAATGAGCT GGAGTTGCTC ATGTGTGGCC TTGGAGATGT TGATGTTAAT
    GACTGGAGAC AACATGCTAT TTACAAGAAT GGCTATTGCC CAAATCACCC AGTCATTCAG
    TGGTTCTGGA AGGCTGTGTT ATTGATGGAT GCTGAGAAGC GTATCCGGTT ACTTCAGTTT
    GTTACCGGGA CCTCCCGGGT ACCTATGAAT GGATTTGCTG AGCTTTATGG TTCAAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTCGATTT ACCTCCATAT GAATCTTTCG AAGATTTAAG AGAGAAACTT
    CTCATGGCCG TTGAAAACGC CCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915595.1
    CDS31..3855
    Translation

    Target ORF information:

    RefSeq Version XM_029059762.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGGAAGATCC ACGACTGAAA TTTCCAGTAC ATTTGCGATC AAAAGCATCT
    TTGAACCCTA ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGACGAACGT TTTATATAGA TCATAATAGT AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTTAA ACAGAAATAT
    GACTATTTTA GGAAGAAGTT AAAGAAACCA GCTGATATTC CAAATAGATT TGAAATGAAG
    CTCCACAGAA ATAACATATT TGAAGAATCA TACAGGAGGA TCATGTCAGT GAAGAGACCT
    GATGTACTAA AAGCCAGGCT ATGGATTGAA TTTGAATCTG AAAAAGGACT AGATTATGGG
    GGCGTGGCCA GAGAATGGTT CTTCCTATTG TCTAAGGAAA TGTTCAATCC TTACTACGGT
    CTCTTTGAGT ACTCCGCAAC GGACAACTAC ACACTTCAAA TCAATCCCAA TTCAGGCCTT
    TGTAATGAAG ATCATCTGTC CTATTTCACT TTTATTGGAA GGGTTGCTGG GCTGGCAGTG
    TTTCATGGGA AGCTCTTAGA TGGATTCTTC ATTAGACCCT TTTACAAAAT GATGTTGGGG
    AAGCAAATAA CATTGAATGA CATGGAGTCA GTGGACAGTG AATATTATAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GATCTTATGT TTTGCATAGA TGAAGAAAAT
    TTTGGACAGA CATACCAAGT GGATTTGAAA CCCAACGGAT CTGAAATTAT GGTAACAAAT
    GAAAATAAAA GGGAATATAT TGACCTAGTC ATCCAGTGGA GATTTGTAAA CCGAGTTCAG
    AAACAAATGA ATGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTACAGA TTTGATTAAA
    ATTTTTGATG AGAATGAGCT GGAGTTGCTC ATGTGTGGCC TTGGAGATGT TGATGTTAAT
    GACTGGAGAC AACATGCTAT TTACAAGAAT GGCTATTGCC CAAATCACCC AGTCATTCAG
    TGGTTCTGGA AGGCTGTGTT ATTGATGGAT GCTGAGAAGC GTATCCGGTT ACTTCAGTTT
    GTTACCGGGA CCTCCCGGGT ACCTATGAAT GGATTTGCTG AGCTTTATGG TTCAAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTCGATTT ACCTCCATAT GAATCTTTCG AAGATTTAAG AGAGAAACTT
    CTCATGGCCG TTGAAAACGC CCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125292
    Clone ID Related Accession (Same CDS sequence) XM_029059763.1
    Accession Version XM_029059763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGGAAGATCC ACGACTGAAA TTTCCAGTAC ATTTGCGATC AAAAGCATCT
    TTGAACCCTA ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGACGAACGT TTTATATAGA TCATAATAGT AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTTAA ACAGAAATAT
    GACTATTTTA GGAAGAAGTT AAAGAAACCA GCTGATATTC CAAATAGATT TGAAATGAAG
    CTCCACAGAA ATAACATATT TGAAGAATCA TACAGGAGGA TCATGTCAGT GAAGAGACCT
    GATGTACTAA AAGCCAGGCT ATGGATTGAA TTTGAATCTG AAAAAGGACT AGATTATGGG
    GGCGTGGCCA GAGAATGGTT CTTCCTATTG TCTAAGGAAA TGTTCAATCC TTACTACGGT
    CTCTTTGAGT ACTCCGCAAC GGACAACTAC ACACTTCAAA TCAATCCCAA TTCAGGCCTT
    TGTAATGAAG ATCATCTGTC CTATTTCACT TTTATTGGAA GGGTTGCTGG GCTGGCAGTG
    TTTCATGGGA AGCTCTTAGA TGGATTCTTC ATTAGACCCT TTTACAAAAT GATGTTGGGG
    AAGCAAATAA CATTGAATGA CATGGAGTCA GTGGACAGTG AATATTATAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GATCTTATGT TTTGCATAGA TGAAGAAAAT
    TTTGGACAGA CATACCAAGT GGATTTGAAA CCCAACGGAT CTGAAATTAT GGTAACAAAT
    GAAAATAAAA GGGAATATAT TGACCTAGTC ATCCAGTGGA GATTTGTAAA CCGAGTTCAG
    AAACAAATGA ATGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTACAGA TTTGATTAAA
    ATTTTTGATG AGAATGAGCT GGAGTTGCTC ATGTGTGGCC TTGGAGATGT TGATGTTAAT
    GACTGGAGAC AACATGCTAT TTACAAGAAT GGCTATTGCC CAAATCACCC AGTCATTCAG
    TGGTTCTGGA AGGCTGTGTT ATTGATGGAT GCTGAGAAGC GTATCCGGTT ACTTCAGTTT
    GTTACCGGGA CCTCCCGGGT ACCTATGAAT GGATTTGCTG AGCTTTATGG TTCAAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTCGATTT ACCTCCATAT GAATCTTTCG AAGATTTAAG AGAGAAACTT
    CTCATGGCCG TTGAAAACGC CCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915596.1
    CDS30..3794
    Translation

    Target ORF information:

    RefSeq Version XM_029059763.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGGTGCC
    AAAGATTCTC CCGTTCGCCG AGCTGTGAAG GACACCCTTT CCAATCCACA GTCCCCACAG
    CCTTCACCTT ACAATTCTCC AAAGCCTCAG CACAAAGTGA CACAAAGCTT CTTGCCACCT
    GGTTGGGAGA TGCGGATTGC TCCAAATGGC CGGCCCTTCT TCATTGATCA TAATACAAAG
    ACGACCACAT GGGAAGATCC ACGACTGAAA TTTCCAGTAC ATTTGCGATC AAAAGCATCT
    TTGAACCCTA ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AAGAAAGAAT TCACTTGGAT
    GGACGAACGT TTTATATAGA TCATAATAGT AAAATTACTC AGTGGGAAGA CCCAAGACTG
    CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTTAA ACAGAAATAT
    GACTATTTTA GGAAGAAGTT AAAGAAACCA GCTGATATTC CAAATAGATT TGAAATGAAG
    CTCCACAGAA ATAACATATT TGAAGAATCA TACAGGAGGA TCATGTCAGT GAAGAGACCT
    GATGTACTAA AAGCCAGGCT ATGGATTGAA TTTGAATCTG AAAAAGGACT AGATTATGGG
    GGCGTGGCCA GAGAATGGTT CTTCCTATTG TCTAAGGAAA TGTTCAATCC TTACTACGGT
    CTCTTTGAGT ACTCCGCAAC GGACAACTAC ACACTTCAAA TCAATCCCAA TTCAGGCCTT
    TGTAATGAAG ATCATCTGTC CTATTTCACT TTTATTGGAA GGGTTGCTGG GCTGGCAGTG
    TTTCATGGGA AGCTCTTAGA TGGATTCTTC ATTAGACCCT TTTACAAAAT GATGTTGGGG
    AAGCAAATAA CATTGAATGA CATGGAGTCA GTGGACAGTG AATATTATAA CTCTTTGAAA
    TGGATCTTGG AAAATGATCC TACTGAACTG GATCTTATGT TTTGCATAGA TGAAGAAAAT
    TTTGGACAGA CATACCAAGT GGATTTGAAA CCCAACGGAT CTGAAATTAT GGTAACAAAT
    GAAAATAAAA GGGAATATAT TGACCTAGTC ATCCAGTGGA GATTTGTAAA CCGAGTTCAG
    AAACAAATGA ATGCCTTCTT GGAGGGATTC ACAGAACTGC TTCCTACAGA TTTGATTAAA
    ATTTTTGATG AGAATGAGCT GGAGTTGCTC ATGTGTGGCC TTGGAGATGT TGATGTTAAT
    GACTGGAGAC AACATGCTAT TTACAAGAAT GGCTATTGCC CAAATCACCC AGTCATTCAG
    TGGTTCTGGA AGGCTGTGTT ATTGATGGAT GCTGAGAAGC GTATCCGGTT ACTTCAGTTT
    GTTACCGGGA CCTCCCGGGT ACCTATGAAT GGATTTGCTG AGCTTTATGG TTCAAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCCTCGATTT ACCTCCATAT GAATCTTTCG AAGATTTAAG AGAGAAACTT
    CTCATGGCCG TTGAAAACGC CCAAGGATTT GAAGGGGTGG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125293
    Clone ID Related Accession (Same CDS sequence) XM_029059765.1
    Accession Version XM_029059765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGAAGAT
    CCACGACTGA AATTTCCAGT ACATTTGCGA TCAAAAGCAT CTTTGAACCC TAATGATTTG
    GGCCCTCTTC CTCCTGGTTG GGAAGAAAGA ATTCACTTGG ATGGACGAAC GTTTTATATA
    GATCATAGAA GCCAGGTGTT GATATGTGGA GACATTGATA CCAGAGACAT AGTGCCTTCC
    TACGATAGTA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCAGCTGTTC CCTATTCCAG AGAGTTTAAA CAGAAATATG ACTATTTTAG GAAGAAGTTA
    AAGAAACCAG CTGATATTCC AAATAGATTT GAAATGAAGC TCCACAGAAA TAACATATTT
    GAAGAATCAT ACAGGAGGAT CATGTCAGTG AAGAGACCTG ATGTACTAAA AGCCAGGCTA
    TGGATTGAAT TTGAATCTGA AAAAGGACTA GATTATGGGG GCGTGGCCAG AGAATGGTTC
    TTCCTATTGT CTAAGGAAAT GTTCAATCCT TACTACGGTC TCTTTGAGTA CTCCGCAACG
    GACAACTACA CACTTCAAAT CAATCCCAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC
    TATTTCACTT TTATTGGAAG GGTTGCTGGG CTGGCAGTGT TTCATGGGAA GCTCTTAGAT
    GGATTCTTCA TTAGACCCTT TTACAAAATG ATGTTGGGGA AGCAAATAAC ATTGAATGAC
    ATGGAGTCAG TGGACAGTGA ATATTATAAC TCTTTGAAAT GGATCTTGGA AAATGATCCT
    ACTGAACTGG ATCTTATGTT TTGCATAGAT GAAGAAAATT TTGGACAGAC ATACCAAGTG
    GATTTGAAAC CCAACGGATC TGAAATTATG GTAACAAATG AAAATAAAAG GGAATATATT
    GACCTAGTCA TCCAGTGGAG ATTTGTAAAC CGAGTTCAGA AACAAATGAA TGCCTTCTTG
    GAGGGATTCA CAGAACTGCT TCCTACAGAT TTGATTAAAA TTTTTGATGA GAATGAGCTG
    GAGTTGCTCA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATGCTATT
    TACAAGAATG GCTATTGCCC AAATCACCCA GTCATTCAGT GGTTCTGGAA GGCTGTGTTA
    TTGATGGATG CTGAGAAGCG TATCCGGTTA CTTCAGTTTG TTACCGGGAC CTCCCGGGTA
    CCTATGAATG GATTTGCTGA GCTTTATGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGAA GTCCTGAAAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTCGATTTA
    CCTCCATATG AATCTTTCGA AGATTTAAGA GAGAAACTTC TCATGGCCGT TGAAAACGCC
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915598.1
    CDS33..3716
    Translation

    Target ORF information:

    RefSeq Version XM_029059765.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGCTTGCT
    GAAGATGGTG CTACCGGGTC AGCCGCAAAC AGTAACAACC ACCTAAGTGA ACCACAGATA
    CGTCGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTGT CTGCCCCCCT GGAGGAAGAT
    CCACGACTGA AATTTCCAGT ACATTTGCGA TCAAAAGCAT CTTTGAACCC TAATGATTTG
    GGCCCTCTTC CTCCTGGTTG GGAAGAAAGA ATTCACTTGG ATGGACGAAC GTTTTATATA
    GATCATAGAA GCCAGGTGTT GATATGTGGA GACATTGATA CCAGAGACAT AGTGCCTTCC
    TACGATAGTA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCAGCTGTTC CCTATTCCAG AGAGTTTAAA CAGAAATATG ACTATTTTAG GAAGAAGTTA
    AAGAAACCAG CTGATATTCC AAATAGATTT GAAATGAAGC TCCACAGAAA TAACATATTT
    GAAGAATCAT ACAGGAGGAT CATGTCAGTG AAGAGACCTG ATGTACTAAA AGCCAGGCTA
    TGGATTGAAT TTGAATCTGA AAAAGGACTA GATTATGGGG GCGTGGCCAG AGAATGGTTC
    TTCCTATTGT CTAAGGAAAT GTTCAATCCT TACTACGGTC TCTTTGAGTA CTCCGCAACG
    GACAACTACA CACTTCAAAT CAATCCCAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC
    TATTTCACTT TTATTGGAAG GGTTGCTGGG CTGGCAGTGT TTCATGGGAA GCTCTTAGAT
    GGATTCTTCA TTAGACCCTT TTACAAAATG ATGTTGGGGA AGCAAATAAC ATTGAATGAC
    ATGGAGTCAG TGGACAGTGA ATATTATAAC TCTTTGAAAT GGATCTTGGA AAATGATCCT
    ACTGAACTGG ATCTTATGTT TTGCATAGAT GAAGAAAATT TTGGACAGAC ATACCAAGTG
    GATTTGAAAC CCAACGGATC TGAAATTATG GTAACAAATG AAAATAAAAG GGAATATATT
    GACCTAGTCA TCCAGTGGAG ATTTGTAAAC CGAGTTCAGA AACAAATGAA TGCCTTCTTG
    GAGGGATTCA CAGAACTGCT TCCTACAGAT TTGATTAAAA TTTTTGATGA GAATGAGCTG
    GAGTTGCTCA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATGCTATT
    TACAAGAATG GCTATTGCCC AAATCACCCA GTCATTCAGT GGTTCTGGAA GGCTGTGTTA
    TTGATGGATG CTGAGAAGCG TATCCGGTTA CTTCAGTTTG TTACCGGGAC CTCCCGGGTA
    CCTATGAATG GATTTGCTGA GCTTTATGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGAA GTCCTGAAAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTCGATTTA
    CCTCCATATG AATCTTTCGA AGATTTAAGA GAGAAACTTC TCATGGCCGT TGAAAACGCC
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125294
    Clone ID Related Accession (Same CDS sequence) XM_029059766.1
    Accession Version XM_029059766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGGAAGAT
    CCACGACTGA AATTTCCAGT ACATTTGCGA TCAAAAGCAT CTTTGAACCC TAATGATTTG
    GGCCCTCTTC CTCCTGGTTG GGAAGAAAGA ATTCACTTGG ATGGACGAAC GTTTTATATA
    GATCATAGAA GCCAGGTGTT GATATGTGGA GACATTGATA CCAGAGACAT AGTGCCTTCC
    TACGATAGTA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCAGCTGTTC CCTATTCCAG AGAGTTTAAA CAGAAATATG ACTATTTTAG GAAGAAGTTA
    AAGAAACCAG CTGATATTCC AAATAGATTT GAAATGAAGC TCCACAGAAA TAACATATTT
    GAAGAATCAT ACAGGAGGAT CATGTCAGTG AAGAGACCTG ATGTACTAAA AGCCAGGCTA
    TGGATTGAAT TTGAATCTGA AAAAGGACTA GATTATGGGG GCGTGGCCAG AGAATGGTTC
    TTCCTATTGT CTAAGGAAAT GTTCAATCCT TACTACGGTC TCTTTGAGTA CTCCGCAACG
    GACAACTACA CACTTCAAAT CAATCCCAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC
    TATTTCACTT TTATTGGAAG GGTTGCTGGG CTGGCAGTGT TTCATGGGAA GCTCTTAGAT
    GGATTCTTCA TTAGACCCTT TTACAAAATG ATGTTGGGGA AGCAAATAAC ATTGAATGAC
    ATGGAGTCAG TGGACAGTGA ATATTATAAC TCTTTGAAAT GGATCTTGGA AAATGATCCT
    ACTGAACTGG ATCTTATGTT TTGCATAGAT GAAGAAAATT TTGGACAGAC ATACCAAGTG
    GATTTGAAAC CCAACGGATC TGAAATTATG GTAACAAATG AAAATAAAAG GGAATATATT
    GACCTAGTCA TCCAGTGGAG ATTTGTAAAC CGAGTTCAGA AACAAATGAA TGCCTTCTTG
    GAGGGATTCA CAGAACTGCT TCCTACAGAT TTGATTAAAA TTTTTGATGA GAATGAGCTG
    GAGTTGCTCA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATGCTATT
    TACAAGAATG GCTATTGCCC AAATCACCCA GTCATTCAGT GGTTCTGGAA GGCTGTGTTA
    TTGATGGATG CTGAGAAGCG TATCCGGTTA CTTCAGTTTG TTACCGGGAC CTCCCGGGTA
    CCTATGAATG GATTTGCTGA GCTTTATGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGAA GTCCTGAAAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTCGATTTA
    CCTCCATATG AATCTTTCGA AGATTTAAGA GAGAAACTTC TCATGGCCGT TGAAAACGCC
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915599.1
    CDS32..3595
    Translation

    Target ORF information:

    RefSeq Version XM_029059766.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGGAAGAT
    CCACGACTGA AATTTCCAGT ACATTTGCGA TCAAAAGCAT CTTTGAACCC TAATGATTTG
    GGCCCTCTTC CTCCTGGTTG GGAAGAAAGA ATTCACTTGG ATGGACGAAC GTTTTATATA
    GATCATAGAA GCCAGGTGTT GATATGTGGA GACATTGATA CCAGAGACAT AGTGCCTTCC
    TACGATAGTA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT
    CCAGCTGTTC CCTATTCCAG AGAGTTTAAA CAGAAATATG ACTATTTTAG GAAGAAGTTA
    AAGAAACCAG CTGATATTCC AAATAGATTT GAAATGAAGC TCCACAGAAA TAACATATTT
    GAAGAATCAT ACAGGAGGAT CATGTCAGTG AAGAGACCTG ATGTACTAAA AGCCAGGCTA
    TGGATTGAAT TTGAATCTGA AAAAGGACTA GATTATGGGG GCGTGGCCAG AGAATGGTTC
    TTCCTATTGT CTAAGGAAAT GTTCAATCCT TACTACGGTC TCTTTGAGTA CTCCGCAACG
    GACAACTACA CACTTCAAAT CAATCCCAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC
    TATTTCACTT TTATTGGAAG GGTTGCTGGG CTGGCAGTGT TTCATGGGAA GCTCTTAGAT
    GGATTCTTCA TTAGACCCTT TTACAAAATG ATGTTGGGGA AGCAAATAAC ATTGAATGAC
    ATGGAGTCAG TGGACAGTGA ATATTATAAC TCTTTGAAAT GGATCTTGGA AAATGATCCT
    ACTGAACTGG ATCTTATGTT TTGCATAGAT GAAGAAAATT TTGGACAGAC ATACCAAGTG
    GATTTGAAAC CCAACGGATC TGAAATTATG GTAACAAATG AAAATAAAAG GGAATATATT
    GACCTAGTCA TCCAGTGGAG ATTTGTAAAC CGAGTTCAGA AACAAATGAA TGCCTTCTTG
    GAGGGATTCA CAGAACTGCT TCCTACAGAT TTGATTAAAA TTTTTGATGA GAATGAGCTG
    GAGTTGCTCA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATGCTATT
    TACAAGAATG GCTATTGCCC AAATCACCCA GTCATTCAGT GGTTCTGGAA GGCTGTGTTA
    TTGATGGATG CTGAGAAGCG TATCCGGTTA CTTCAGTTTG TTACCGGGAC CTCCCGGGTA
    CCTATGAATG GATTTGCTGA GCTTTATGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG
    CAATGGGGAA GTCCTGAAAA ACTGCCCAGA GCTCACACAT GCTTTAATCG CCTCGATTTA
    CCTCCATATG AATCTTTCGA AGATTTAAGA GAGAAACTTC TCATGGCCGT TGAAAACGCC
    CAAGGATTTG AAGGGGTGGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125295
    Clone ID Related Accession (Same CDS sequence) XM_029059767.1
    Accession Version XM_029059767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGGAAGAT
    CCACGACTGA AATTTCCAGT ACATTTGCGA TCAAAAGCAT CTTTGAACCC TAATGATTTG
    GGCCCTCTTC CTCCTGGTTG GGAAGAAAGA ATTCACTTGG ATGGACGAAC GTTTTATATA
    GATCATAATA GTAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCAGCTG TTCCCTATTC CAGAGAGTTT AAACAGAAAT ATGACTATTT TAGGAAGAAG
    TTAAAGAAAC CAGCTGATAT TCCAAATAGA TTTGAAATGA AGCTCCACAG AAATAACATA
    TTTGAAGAAT CATACAGGAG GATCATGTCA GTGAAGAGAC CTGATGTACT AAAAGCCAGG
    CTATGGATTG AATTTGAATC TGAAAAAGGA CTAGATTATG GGGGCGTGGC CAGAGAATGG
    TTCTTCCTAT TGTCTAAGGA AATGTTCAAT CCTTACTACG GTCTCTTTGA GTACTCCGCA
    ACGGACAACT ACACACTTCA AATCAATCCC AATTCAGGCC TTTGTAATGA AGATCATCTG
    TCCTATTTCA CTTTTATTGG AAGGGTTGCT GGGCTGGCAG TGTTTCATGG GAAGCTCTTA
    GATGGATTCT TCATTAGACC CTTTTACAAA ATGATGTTGG GGAAGCAAAT AACATTGAAT
    GACATGGAGT CAGTGGACAG TGAATATTAT AACTCTTTGA AATGGATCTT GGAAAATGAT
    CCTACTGAAC TGGATCTTAT GTTTTGCATA GATGAAGAAA ATTTTGGACA GACATACCAA
    GTGGATTTGA AACCCAACGG ATCTGAAATT ATGGTAACAA ATGAAAATAA AAGGGAATAT
    ATTGACCTAG TCATCCAGTG GAGATTTGTA AACCGAGTTC AGAAACAAAT GAATGCCTTC
    TTGGAGGGAT TCACAGAACT GCTTCCTACA GATTTGATTA AAATTTTTGA TGAGAATGAG
    CTGGAGTTGC TCATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATGCT
    ATTTACAAGA ATGGCTATTG CCCAAATCAC CCAGTCATTC AGTGGTTCTG GAAGGCTGTG
    TTATTGATGG ATGCTGAGAA GCGTATCCGG TTACTTCAGT TTGTTACCGG GACCTCCCGG
    GTACCTATGA ATGGATTTGC TGAGCTTTAT GGTTCAAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CATGCTTTAA TCGCCTCGAT
    TTACCTCCAT ATGAATCTTT CGAAGATTTA AGAGAGAAAC TTCTCATGGC CGTTGAAAAC
    GCCCAAGGAT TTGAAGGGGT GGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915600.1
    CDS31..3537
    Translation

    Target ORF information:

    RefSeq Version XM_029059767.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCATC GGCTGCGACT TTATTTTGGT TCCGGTCGAA GCAACACGGC CCCAGAATCA 
    GAGATCCTAG ACCAAGAAAG AGAAGACGAC TTTTGCATGG CATTCCACAC TCTGCCAAGG
    AGAAACGGTC CTCACGCTTT TCCTCGACAT GGAGCGGAAT ATGGGAACGC TGGGCTGCAA
    GAAGGAGTGG GAGCACTAAA ACGAAGTACT TCCATGTTTA TTCCTCAGCT GTTGAATAAC
    ATCGACGCCC GCCCTACGAG AAGCTCGTCT GTGCAGATCT CCCTTCAGCG CAAAGCTCTG
    AACGGACATA CCGATGAGTG TGGGGCACCA GAGCCTCCTG AGGAGGCCCT TCCGTGTAAA
    TTTTCGGACT TCCGGGAGAA TTTCTCGTTA CCTGCAAGGA GGCCTCCTTC ATCTCCAAGC
    AGCCCACGAG GATCCCCAGA AGGGGAAAAC CCAGAGAGTT CCTCTTCCTC CAGTCTGGCA
    GGGGATGCAG GGCAGCCGGT CAATGGAGCC AGCTCTGGCA GTCATAGGCT GTTTCGTGCA
    ATTCCTTCCG ATAACCAGGA TGAGGCTGCC CCCACCACCG CCCAAGGAGC GGAACCAGGT
    GAAGCCGCTT CGGGCGTGAA CCTAAGTGGA GTGAGAATTC AGCATCGTGC CTCCAGTGTC
    GACATGCGTC AGGTTAGGCT GCTGCCTTTT GGGTCTGACT GTCCGAATAA TCCTCCTGCC
    TCTGAACCGA GGCGATGGTC TTTGCAGCAC GTGCCCGACG TGTCAGCGAG TACCGGGAAA
    AGGTGTTTTG TTTTCCAGCT GCAACAGCCT CAAGCAAACG CTCCGAGCCC CGGTAGTGAT
    TGCAATTTCG GTTTCACCGG AACCAAGGGG GACAGGTTGG TTAGGTATCC CCGGATTAGG
    CTGGAGAGGA GCACCTCTTA TCCCGTCCAG CCGCGACCGC TACAGATTAC TCCCACCGAA
    GATGGCATAA GTTCAGCAAC TCTTAGCAGT AGCAGTAATG GGCCTGAAAT CCACAGGAGT
    AATTCAGTAG AGAGGATCCC GCAGGGACCA GGCTGTATGT TTAAGATCAG GCCAGATCAA
    AACTCAAGGC AACAGCATTT TAGAATTCTT GTCACCCGTG GCTCCGAAGA ACCCGAGCGA
    GCCGCAGGGG AGGGTAGTTC CGGAGATCCT GCCACTTCGG TAGTGAGTGC TCCGACGAGA
    GACGACTTCT TGGGGCAGGT GGACGTGCCT CTTAATCATC TACCGACAGA AGACCCAACC
    ATGGAACGAC CCTACACCTT TAAGGATTTT CTCCTTAGGC CAAGGAGTCA CAAATCCCGG
    GTAAAGGGCT TCTTGAGATT GAAGATGGCC TACATGCCGA AGAATGGAGG ACAAGATGAA
    GAAAATAATG AACAAAGGGA AGAGTTGGAG CACGGGTGGG AGGTCGTTGA CTCCAATGAC
    TCAGCTTCCC AACACCAGGA GGAATTGCCC CCACCACCTC TTCCCCCCGG ATGGGAAGAG
    AAAGTTGATA ACTTGGGGCG AACTTATTAC GTCAACCACA ACAACAGGAC TACCCAGTGG
    CACCGACCAA GTTTAATTGA CGTGTCTTCT GAATCGGATA ATAATATCAG GCAGATAAAT
    CAGGAAGCAG CCCACAGACG ATTCCGCTCT CGTAGACACA TCAGTGAAGA TTTAGAACCT
    GAACCTGCAG AAGGTGGAGA TGCTCCTGAG CCCTGGGAAA CCATCTCAGA GGAAGTGAAC
    ATAGCCAGTG ACTCTATGAG TCTGGCATTG CCACCACCTC CTGCTTCTCC AGTCTCTCGG
    ACCAGTCCTC AAGAGCTGTC AGAGGAATTG AACAGAAGAC TTCAGATCAC TCCGGATTCT
    AATGGGGAAC AATTCAGTTC TTTAATTCAA AGGGAACCTC CTTCAAGACT GAGGTCATGC
    AGTGTAACAG ATACGGTGGC TGAACAGGCC CATCTACCCC TGCAGAGTGT CCCAACTAGG
    AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAGGAGCCAA CGCCATCAGT GGCCTACGTA
    CATACTACGC CGGGACTACC TTCAGGCTGG GAAGAACGGA AAGATGCTAA GGGACGCACG
    TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GACCTATCAT GCAGGAAGAT
    CCACGACTGA AATTTCCAGT ACATTTGCGA TCAAAAGCAT CTTTGAACCC TAATGATTTG
    GGCCCTCTTC CTCCTGGTTG GGAAGAAAGA ATTCACTTGG ATGGACGAAC GTTTTATATA
    GATCATAATA GTAAAATTAC TCAGTGGGAA GACCCAAGAC TGCAGAACCC AGCTATTACT
    GGTCCAGCTG TTCCCTATTC CAGAGAGTTT AAACAGAAAT ATGACTATTT TAGGAAGAAG
    TTAAAGAAAC CAGCTGATAT TCCAAATAGA TTTGAAATGA AGCTCCACAG AAATAACATA
    TTTGAAGAAT CATACAGGAG GATCATGTCA GTGAAGAGAC CTGATGTACT AAAAGCCAGG
    CTATGGATTG AATTTGAATC TGAAAAAGGA CTAGATTATG GGGGCGTGGC CAGAGAATGG
    TTCTTCCTAT TGTCTAAGGA AATGTTCAAT CCTTACTACG GTCTCTTTGA GTACTCCGCA
    ACGGACAACT ACACACTTCA AATCAATCCC AATTCAGGCC TTTGTAATGA AGATCATCTG
    TCCTATTTCA CTTTTATTGG AAGGGTTGCT GGGCTGGCAG TGTTTCATGG GAAGCTCTTA
    GATGGATTCT TCATTAGACC CTTTTACAAA ATGATGTTGG GGAAGCAAAT AACATTGAAT
    GACATGGAGT CAGTGGACAG TGAATATTAT AACTCTTTGA AATGGATCTT GGAAAATGAT
    CCTACTGAAC TGGATCTTAT GTTTTGCATA GATGAAGAAA ATTTTGGACA GACATACCAA
    GTGGATTTGA AACCCAACGG ATCTGAAATT ATGGTAACAA ATGAAAATAA AAGGGAATAT
    ATTGACCTAG TCATCCAGTG GAGATTTGTA AACCGAGTTC AGAAACAAAT GAATGCCTTC
    TTGGAGGGAT TCACAGAACT GCTTCCTACA GATTTGATTA AAATTTTTGA TGAGAATGAG
    CTGGAGTTGC TCATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATGCT
    ATTTACAAGA ATGGCTATTG CCCAAATCAC CCAGTCATTC AGTGGTTCTG GAAGGCTGTG
    TTATTGATGG ATGCTGAGAA GCGTATCCGG TTACTTCAGT TTGTTACCGG GACCTCCCGG
    GTACCTATGA ATGGATTTGC TGAGCTTTAT GGTTCAAATG GTCCTCAGCT GTTTACAATA
    GAGCAATGGG GAAGTCCTGA AAAACTGCCC AGAGCTCACA CATGCTTTAA TCGCCTCGAT
    TTACCTCCAT ATGAATCTTT CGAAGATTTA AGAGAGAAAC TTCTCATGGC CGTTGAAAAC
    GCCCAAGGAT TTGAAGGGGT GGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125296
    Clone ID Related Accession (Same CDS sequence) XM_029059768.1
    Accession Version XM_029059768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGACCG GGCTGGGAGA GCCGGTGTAT GGACTTTCTG AAGACGAGGG GGAGTCGCGC 
    ATCCTCAGAG TAAAAGTTGT TTCTGGAATT GACCTGGCGA AGAAGGACAT CTTTGGAGCC
    AGTGACCCGT ATGTGAAACT TTCATTGTAT GTAGCTGATG AGAATCGAGA ACTTGCTTTG
    GTACAGACCA AAACCATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTCATTTT
    AGAGTGAACC CAACTAATCA TAGGCTCTTG TTTGAAGTGT TTGATGAAAA CAGATTGACG
    AGAGACGACT TCTTGGGGCA GGTGGACGTG CCTCTTAATC ATCTACCGAC AGAAGACCCA
    ACCATGGAAC GACCCTACAC CTTTAAGGAT TTTCTCCTTA GGCCAAGGAG TCACAAATCC
    CGGGTAAAGG GCTTCTTGAG ATTGAAGATG GCCTACATGC CGAAGAATGG AGGACAAGAT
    GAAGAAAATA ATGAACAAAG GGAAGAGTTG GAGCACGGGT GGGAGGTCGT TGACTCCAAT
    GACTCAGCTT CCCAACACCA GGAGGAATTG CCCCCACCAC CTCTTCCCCC CGGATGGGAA
    GAGAAAGTTG ATAACTTGGG GCGAACTTAT TACGTCAACC ACAACAACAG GACTACCCAG
    TGGCACCGAC CAAGTTTAAT TGACGTGTCT TCTGAATCGG ATAATAATAT CAGGCAGATA
    AATCAGGAAG CAGCCCACAG ACGATTCCGC TCTCGTAGAC ACATCAGTGA AGATTTAGAA
    CCTGAACCTG CAGAAGGTGG AGATGCTCCT GAGCCCTGGG AAACCATCTC AGAGGAAGTG
    AACATAGCCA GTGACTCTAT GAGTCTGGCA TTGCCACCAC CTCCTGCTTC TCCAGTCTCT
    CGGACCAGTC CTCAAGAGCT GTCAGAGGAA TTGAACAGAA GACTTCAGAT CACTCCGGAT
    TCTAATGGGG AACAATTCAG TTCTTTAATT CAAAGGGAAC CTCCTTCAAG ACTGAGGTCA
    TGCAGTGTAA CAGATACGGT GGCTGAACAG GCCCATCTAC CCCTGCAGAG TGTCCCAACT
    AGGAGAGCTC GTTCTTCAAC TGTCACAGGT GGTGAGGAGC CAACGCCATC AGTGGCCTAC
    GTACATACTA CGCCGGGACT ACCTTCAGGC TGGGAAGAAC GGAAAGATGC TAAGGGACGC
    ACGTACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCTGAAGATG GTGCTACCGG GTCAGCCGCA AACAGTAACA ACCACCTAAG TGAACCACAG
    ATACGTCGGC CTCGTAGCCT CAGTTCACCC ACAGTAACTT TGTCTGCCCC CCTGGAGGGT
    GCCAAAGATT CTCCCGTTCG CCGAGCTGTG AAGGACACCC TTTCCAATCC ACAGTCCCCA
    CAGCCTTCAC CTTACAATTC TCCAAAGCCT CAGCACAAAG TGACACAAAG CTTCTTGCCA
    CCTGGTTGGG AGATGCGGAT TGCTCCAAAT GGCCGGCCCT TCTTCATTGA TCATAATACA
    AAGACGACCA CATGGCTGAT ACCCAGCAGA GTACTGATCA CACGGAGCTG TACTGATGTT
    GAAGTTACTG GTGAAGAGGC AGAGAATGGG GGCAACCAGC CATGCTCTCT GCTCAGTTCG
    GTTAGAAACA CAGCATCAAA AGAAGATCCA CGACTGAAAT TTCCAGTACA TTTGCGATCA
    AAAGCATCTT TGAACCCTAA TGATTTGGGC CCTCTTCCTC CTGGTTGGGA AGAAAGAATT
    CACTTGGATG GACGAACGTT TTATATAGAT CATAGAAGCC AGGTGTTGAT ATGTGGAGAC
    ATTGATACCA GAGACATAGT GCCTTCCTAC GATAGTAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCA GCTGTTCCCT ATTCCAGAGA GTTTAAACAG
    AAATATGACT ATTTTAGGAA GAAGTTAAAG AAACCAGCTG ATATTCCAAA TAGATTTGAA
    ATGAAGCTCC ACAGAAATAA CATATTTGAA GAATCATACA GGAGGATCAT GTCAGTGAAG
    AGACCTGATG TACTAAAAGC CAGGCTATGG ATTGAATTTG AATCTGAAAA AGGACTAGAT
    TATGGGGGCG TGGCCAGAGA ATGGTTCTTC CTATTGTCTA AGGAAATGTT CAATCCTTAC
    TACGGTCTCT TTGAGTACTC CGCAACGGAC AACTACACAC TTCAAATCAA TCCCAATTCA
    GGCCTTTGTA ATGAAGATCA TCTGTCCTAT TTCACTTTTA TTGGAAGGGT TGCTGGGCTG
    GCAGTGTTTC ATGGGAAGCT CTTAGATGGA TTCTTCATTA GACCCTTTTA CAAAATGATG
    TTGGGGAAGC AAATAACATT GAATGACATG GAGTCAGTGG ACAGTGAATA TTATAACTCT
    TTGAAATGGA TCTTGGAAAA TGATCCTACT GAACTGGATC TTATGTTTTG CATAGATGAA
    GAAAATTTTG GACAGACATA CCAAGTGGAT TTGAAACCCA ACGGATCTGA AATTATGGTA
    ACAAATGAAA ATAAAAGGGA ATATATTGAC CTAGTCATCC AGTGGAGATT TGTAAACCGA
    GTTCAGAAAC AAATGAATGC CTTCTTGGAG GGATTCACAG AACTGCTTCC TACAGATTTG
    ATTAAAATTT TTGATGAGAA TGAGCTGGAG TTGCTCATGT GTGGCCTTGG AGATGTTGAT
    GTTAATGACT GGAGACAACA TGCTATTTAC AAGAATGGCT ATTGCCCAAA TCACCCAGTC
    ATTCAGTGGT TCTGGAAGGC TGTGTTATTG ATGGATGCTG AGAAGCGTAT CCGGTTACTT
    CAGTTTGTTA CCGGGACCTC CCGGGTACCT ATGAATGGAT TTGCTGAGCT TTATGGTTCA
    AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT
    CACACATGCT TTAATCGCCT CGATTTACCT CCATATGAAT CTTTCGAAGA TTTAAGAGAG
    AAACTTCTCA TGGCCGTTGA AAACGCCCAA GGATTTGAAG GGGTGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028915601.1
    CDS109..3219
    Translation

    Target ORF information:

    RefSeq Version XM_029059768.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus NEDD4 like E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029059768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGACCG GGCTGGGAGA GCCGGTGTAT GGACTTTCTG AAGACGAGGG GGAGTCGCGC 
    ATCCTCAGAG TAAAAGTTGT TTCTGGAATT GACCTGGCGA AGAAGGACAT CTTTGGAGCC
    AGTGACCCGT ATGTGAAACT TTCATTGTAT GTAGCTGATG AGAATCGAGA ACTTGCTTTG
    GTACAGACCA AAACCATTAA AAAGACACTG AACCCAAAAT GGAATGAAGA ATTTCATTTT
    AGAGTGAACC CAACTAATCA TAGGCTCTTG TTTGAAGTGT TTGATGAAAA CAGATTGACG
    AGAGACGACT TCTTGGGGCA GGTGGACGTG CCTCTTAATC ATCTACCGAC AGAAGACCCA
    ACCATGGAAC GACCCTACAC CTTTAAGGAT TTTCTCCTTA GGCCAAGGAG TCACAAATCC
    CGGGTAAAGG GCTTCTTGAG ATTGAAGATG GCCTACATGC CGAAGAATGG AGGACAAGAT
    GAAGAAAATA ATGAACAAAG GGAAGAGTTG GAGCACGGGT GGGAGGTCGT TGACTCCAAT
    GACTCAGCTT CCCAACACCA GGAGGAATTG CCCCCACCAC CTCTTCCCCC CGGATGGGAA
    GAGAAAGTTG ATAACTTGGG GCGAACTTAT TACGTCAACC ACAACAACAG GACTACCCAG
    TGGCACCGAC CAAGTTTAAT TGACGTGTCT TCTGAATCGG ATAATAATAT CAGGCAGATA
    AATCAGGAAG CAGCCCACAG ACGATTCCGC TCTCGTAGAC ACATCAGTGA AGATTTAGAA
    CCTGAACCTG CAGAAGGTGG AGATGCTCCT GAGCCCTGGG AAACCATCTC AGAGGAAGTG
    AACATAGCCA GTGACTCTAT GAGTCTGGCA TTGCCACCAC CTCCTGCTTC TCCAGTCTCT
    CGGACCAGTC CTCAAGAGCT GTCAGAGGAA TTGAACAGAA GACTTCAGAT CACTCCGGAT
    TCTAATGGGG AACAATTCAG TTCTTTAATT CAAAGGGAAC CTCCTTCAAG ACTGAGGTCA
    TGCAGTGTAA CAGATACGGT GGCTGAACAG GCCCATCTAC CCCTGCAGAG TGTCCCAACT
    AGGAGAGCTC GTTCTTCAAC TGTCACAGGT GGTGAGGAGC CAACGCCATC AGTGGCCTAC
    GTACATACTA CGCCGGGACT ACCTTCAGGC TGGGAAGAAC GGAAAGATGC TAAGGGACGC
    ACGTACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGACCTAT CATGCAGCTT
    GCTGAAGATG GTGCTACCGG GTCAGCCGCA AACAGTAACA ACCACCTAAG TGAACCACAG
    ATACGTCGGC CTCGTAGCCT CAGTTCACCC ACAGTAACTT TGTCTGCCCC CCTGGAGGGT
    GCCAAAGATT CTCCCGTTCG CCGAGCTGTG AAGGACACCC TTTCCAATCC ACAGTCCCCA
    CAGCCTTCAC CTTACAATTC TCCAAAGCCT CAGCACAAAG TGACACAAAG CTTCTTGCCA
    CCTGGTTGGG AGATGCGGAT TGCTCCAAAT GGCCGGCCCT TCTTCATTGA TCATAATACA
    AAGACGACCA CATGGCTGAT ACCCAGCAGA GTACTGATCA CACGGAGCTG TACTGATGTT
    GAAGTTACTG GTGAAGAGGC AGAGAATGGG GGCAACCAGC CATGCTCTCT GCTCAGTTCG
    GTTAGAAACA CAGCATCAAA AGAAGATCCA CGACTGAAAT TTCCAGTACA TTTGCGATCA
    AAAGCATCTT TGAACCCTAA TGATTTGGGC CCTCTTCCTC CTGGTTGGGA AGAAAGAATT
    CACTTGGATG GACGAACGTT TTATATAGAT CATAGAAGCC AGGTGTTGAT ATGTGGAGAC
    ATTGATACCA GAGACATAGT GCCTTCCTAC GATAGTAAAA TTACTCAGTG GGAAGACCCA
    AGACTGCAGA ACCCAGCTAT TACTGGTCCA GCTGTTCCCT ATTCCAGAGA GTTTAAACAG
    AAATATGACT ATTTTAGGAA GAAGTTAAAG AAACCAGCTG ATATTCCAAA TAGATTTGAA
    ATGAAGCTCC ACAGAAATAA CATATTTGAA GAATCATACA GGAGGATCAT GTCAGTGAAG
    AGACCTGATG TACTAAAAGC CAGGCTATGG ATTGAATTTG AATCTGAAAA AGGACTAGAT
    TATGGGGGCG TGGCCAGAGA ATGGTTCTTC CTATTGTCTA AGGAAATGTT CAATCCTTAC
    TACGGTCTCT TTGAGTACTC CGCAACGGAC AACTACACAC TTCAAATCAA TCCCAATTCA
    GGCCTTTGTA ATGAAGATCA TCTGTCCTAT TTCACTTTTA TTGGAAGGGT TGCTGGGCTG
    GCAGTGTTTC ATGGGAAGCT CTTAGATGGA TTCTTCATTA GACCCTTTTA CAAAATGATG
    TTGGGGAAGC AAATAACATT GAATGACATG GAGTCAGTGG ACAGTGAATA TTATAACTCT
    TTGAAATGGA TCTTGGAAAA TGATCCTACT GAACTGGATC TTATGTTTTG CATAGATGAA
    GAAAATTTTG GACAGACATA CCAAGTGGAT TTGAAACCCA ACGGATCTGA AATTATGGTA
    ACAAATGAAA ATAAAAGGGA ATATATTGAC CTAGTCATCC AGTGGAGATT TGTAAACCGA
    GTTCAGAAAC AAATGAATGC CTTCTTGGAG GGATTCACAG AACTGCTTCC TACAGATTTG
    ATTAAAATTT TTGATGAGAA TGAGCTGGAG TTGCTCATGT GTGGCCTTGG AGATGTTGAT
    GTTAATGACT GGAGACAACA TGCTATTTAC AAGAATGGCT ATTGCCCAAA TCACCCAGTC
    ATTCAGTGGT TCTGGAAGGC TGTGTTATTG ATGGATGCTG AGAAGCGTAT CCGGTTACTT
    CAGTTTGTTA CCGGGACCTC CCGGGTACCT ATGAATGGAT TTGCTGAGCT TTATGGTTCA
    AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT
    CACACATGCT TTAATCGCCT CGATTTACCT CCATATGAAT CTTTCGAAGA TTTAAGAGAG
    AAACTTCTCA TGGCCGTTGA AAACGCCCAA GGATTTGAAG GGGTGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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