TOPBP1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOPBP1
Entrez Gene ID	100083291
Full Name	DNA topoisomerase II binding protein 1
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007666207.2	XP_007664397.1	LOW QUALITY PROTEIN: DNA topoisomerase 2-binding protein 1
XM_029070875.1	XP_028926708.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_029070876.1	XP_028926709.1	DNA topoisomerase 2-binding protein 1 isoform X2
XM_029070877.1	XP_028926710.1	DNA topoisomerase 2-binding protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following TOPBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc133432	XM_029070875.1 Latest version!	Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc133433	XM_029070876.1 Latest version!	Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc133434	XM_029070877.1 Latest version!	Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc06242	XM_007666207.2 Latest version!	Ornithorhynchus anatinus topoisomerase (DNA) II binding protein 1 (TOPBP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOPBP1 ( XM_029070875.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028926708.1 Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc133432
Clone ID Related Accession (Same CDS sequence)	XM_029070875.1
Accession Version	XM_029070875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041735.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGC TTGGTTGTAG AATTGTGGGA 
CCTCAAGTAG TCCTATTTTG TATGCAGCAC CAGCGATGTG TACCAAGAGC AGACTATCCG 
GTTTTTAATA TGATTATGGC AGATGTAACT ATATCCTGTA CCAGTCTTGA CAAAGAAACA 
AGGGAAGAAG TTCATAAATA TGTTCAGATG ATGGGTGGAC GAGTGTACAG AGATCTTAAT 
GTATCAGTAA CTCATCTTAT TGCTGGAGAA GTAGGCAGCA AAAAATACTT AGTAGCTGCT 
AGTTTAAAGA AACCTATCAT GCGCCCTTCT TGGATAAAAA CGCTATGGGA GAAGTCACAA 
GAAAACAGAA TGATCAGATG TACAGATGTA GACCTAGAAG AGTTTCGGTG CCCTCTATTT 
CTTGGCTGCA CAATTTGTGT GACTGGATTA AGTAGTCTGG ACAGGAAGGA GGTTCAGCGA 
CTAACGGTAG AACACGGCGG CCAATACATG GGACAATTAA AGATGAATGA AACCACTCAC 
CTTGTCGTGC AAGAACCAAA AGGGCAGAAA TATGAATGTG CCAAGAGATG GAATGTACAT 
TGTGTGACCA TCCAGTGGTT TTTCGACAGT ATTGAGAGAG GTTTTTGTCA AGACGAAGTT 
ATGTATAAGA CAGAGCCCAG ACCAGAAACA AAGAACCTGC CAGATACTTC AACTCCTACT 
GGTCAGACCA GCAAAGCCGA TAGTCGTACC CTTTCAGACG TCAGCCATAT TTCTAATATC 
AATTCAAGTT GCGTAAATGA GTCTGCGTTT ACCTCTGCTC TAAACAGCAA ATTGGATTCT 
CTCCTGGAGG ATTTAGAAAA TCTGGATGTC AGCACTTTAC AAGCACCAGA AGATTTACTA 
GACGGTTGTC GAATATATCT CTGTGGATTT AGTGGCCGAA AATTAGATAA GCTGAGAAGG 
CTTATTAATT GTGGTGGTGG AGTTCGTTTT AATCAGCTCA ACGAAGATGT CACTCATGTC 
ATTATAGGAG ACTTTGATGA TGAATTGAAG CACTTTTGGA ATAAGTCAAC ACACAGGCCA 
TATGTAGTAG GAGCAAAATG GCTGCTGGAG TGCTTTAGTA AGGGCTATCT GCTTTCTGAG 
GAGCCATATG TTCACGTGAA CTACCGAGCA GTGGAAATTT CCGTTTCAGA GCAACCTGGA 
ATCAAACCTG CCCTTTCAAA AAGAAATAGC AATTTGCCTA AGAAAGATTC CGTGGCCTCT 
GAGGTGCAGC AGCAAGCGGA TGAGGACCTA CTCTCTCAGT ATGAGAAAAA TGACTCGACA 
TTAGGGGTGG CTGAGAAATC TGAAGCTGGG AAATTTAATG ATTCTACTCA TATCCTTTGG 
AAAGAGGAGG ATCAATCTTC CAGTCACAAT CACCTTCCTG AGGCTTCTAC GATTATTGAA 
AGCGGATTGT TCAGCAAGAA AAGGTTCCTC GTTGTGGGTT TTTCTGAAGA TGATGAGCCT 
TGTATTTCAG ATGCTATAAG AGAAAATGCT GGGAAAGTTG TATCCCTCCA AAAAAGAATC 
ATTGCTGACT ATGCTGTGGT TCCTTTACTG GGGTGTAAAG TGGAGTCAAC TGTAGGAGAA 
GTGGTTACAA ACACATGGCT GGGTATGTGC ATTGAGCATC AGGTCCTGTT TGACCCAGAG 
TCAAATCCTC TTTTCACACC ACTACCTGTA ATGGAAGGGA AAACCCCTTT AGAAGACTGC 
GTTCTTTCAT TCAGCCAATT TACTGGGGCA GAGAGAGACT CCTTGATATA CTTAGCAAGT 
TTGCTTGGAG CGAGAGTTCA AGAGTTCTTC GTACGCAAAG CTAATACCAA AAAAGGCATG 
TTTGCCAGTA CCCACCTGGT GCTGAAAGAA CCTGATGGTT CCAAATATGA AGCGGCAAAA 
AAGTGGAATT TACCTGCAAT CACTGTGTCT TGGCTCTTAC AATCTGCGAG GCTGGGAAAG 
AAAGCTGAAG AAAGCAAGTT TCTGGTCGAG AACGCATCTG CCAAAGCAGA AGAAAGTTCT 
CAAAGTCAGA AACCAACAGA TCAGACTCAA GTATCTGGTT CAGACACACC AGAAAAGTCT 
AGCACACTCT TGGAGAGCAA AAGGAACTCA GCCGTGACAC CTTTGGATAT GAACAGGTTT 
CAGAGTAAAG CTTTTCATAC GGTACTCTCA CAGCAAGCCA GGAAAACCTC AGTTTCCGGA 
GAAGGAAAAC CACTTCAGAA GGAACCGTCG CTACACCTAG ACACACCATC GAAATTTCTG 
TCCAAAGACA AGCTCTTCAA GCCTTCATTT GATGTGAAGG ATGCCTTGGC AGTTTTGGAA 
ACTCCAAGAG GGTGTAGCCA GAGACACAGG AAGTTGAGCA CCCCCCTCTC AGAAGTCATT 
GGCAGGAACC TGAAACTGGC CGTAGCAAAT AGCACTCGTC ACGTAGCGGT TCTTTCCGCA 
AGTCCTCGGC TGGAGGATGC CCAGCTGGAA AAGTCCATAC AGAAAGAAGA ATTCAAACCA 
TTAACTGATG TAGTGATATG CGTCAGTAAG AAACTCAGTA AGAAGCAGAG CGAGCTGAAC 
GGTATTGCAG CATCTCTAGG AGCAGATTAC AGGTGGAGCT TCGATGAAAC GGTGACACAT 
TTCATCTACC AGGGGCGACA GAACGACAGT AATCGAGAAT ATAGATCTGT TAAAGAAAGA 
GGAATACATA TTGTTTCTGA ACACTGGCTT TTACAAAGTG CCCAAGAATA CAGACGCCTT 
CCCGAATCTC TTTACCCACA CACCTACAAT CCCAAAATGA GCCTGGATAT AAGTGCGACA 
CAAGATGAGA GGCTCTATAC CGGTCAGCTT TCATCATCTG ATAAAATACC AAAGGATAAT 
GAGCCTGAGT CTCTATCTAT GGAGGTGAAG AATGTAGAAG ACGGACCCTC TGATCCGATG 
GAGATGGAGA CCGCAAATGA CAATCACCCA AATCCCAAAG GAGTGCTAAC ACAGACCCTG 
GAAATGAGGG AAAACTTCCA GAAGCAGCTG CAAGAAATCA TGTCTGCGAC ATCAATAGCG 
AAACCCCAAG GGCAGAGGGG CTCCCTCTCC AGAAATGGGT GTGACAGCAC TCCTTCAACT 
CCAGACAGCG CGCGTTCTGC TCGAAGTAGT CGCACCCGAG CCTTGGAAGT GCTAAGACAG 
CCTCATCGGA CCCTAACCGA TCTCAACACC GAGCCCTCCC AGAGCGAACA GATCATTTGG 
GACGATCCTA CGGCACGAGA GGAAAGAGCG AGGCTGGCCA GCAACCTGCA GTGGCCCACC 
AGTCCCACGC GACTCTCCGA GCTCCCAGCC GACGTGACAG ACTTGGATGA CTCCCCGTTT 
ACGGACTCCT TACATGACTC TGAAACTTCC GAGCTAGCTG CTGCTGATCC CGAAAAAGCA 
TGGGACACTG AGGACCCGAA GAACCAGATC TCGGAAGAAG CAGAAACCAC AATGAAAGAT 
GACCACCTGA TCCCTACACC ACAAGCCCCC AGCATCGCTT TCCCCCTAGC CAACCCGCCC 
GTGGCTCCAC AACCCAAAGA GAAGACGGTA CGGGAAAAGG AGACTCGTGA AGAGCTGACA 
AAACAATACA TATTCCAGCT CTCCTCTCTC AATCCTCAAG AACGAATCGA CTACTGTCAT 
TTGATCGAGG AACTAGGTGG ATCGGTGCTT GAAAAGCAGT GCTTTGACGC GAGCTGCACG 
CACATCGTTG TGGGTCATCC TCTCCGAAAT GAGAAGTATT TGGCTTCCAT GGCAGCTGGC 
AAGTGGGTCC TTCATCGCTC CTACCTCGAG GCCTGTAGGA CCGCCGGGTG CTTTGTGCAG 
GAGGAAGATT ATGAATGGGG AAGCAGTTCC ATACTCGATG TGCTGACTGG AATCAACCTG 
CAGCAGAAAA AATTAGCCCT GGCAGCAATG AGATGGCGGA AGAAAATCCA GAGAAGACAA 
GAAGAAGCAG GCGTTGTTGA GGGGGCATTT AGTGGCTGGA GGGTAGTTTT AAATGTTGAC 
CAGTCCAGAG AAGCAGGATT CAAGCGTCTT CTTCAGTCAG GAGGGGCAAA GGTGCTGCCG 
GGGCACTCTC CATCTCTCTT TAAAGAAGCC ACACACCTCT TCGCGGACTT CAGTAAACTA 
AAACCAGACG ATACCAGAGT TAATGTGATG GAAGCTGCTG CCCAAAATGT GAACTGCTTG 
AAACCAGAAT ACATTGCAGA TTATCTTATG CAGGAGTTGC CTCCCCCGAT GGAGAATTAC 
TGCCTTCCTG GAGCAGCTGT TCCTCAGAAC AGAAAGGAAC TCGATGCAGG TCTATCCCAG 
AAGAGAAAAG CGCCCGCTGA AAAAAATAAA GTCAAGCGCC TCCGGCTACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028926708.1
CDS	76..4629
Translation	MSSSDREPFFVKFIKSQDNREFFFTALESIKEFQSEEYLQTITEEEALKIKENDRSLYICDPFGGDVFEHLKKLGCRIVGPQVVLFCMQHQRCVPRADYPVFNMIMADVTISCTSLDKETREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAASLKKPIMRPSWIKTLWEKSQENRMIRCTDVDLEEFRCPLFLGCTICVTGLSSLDRKEVQRLTVEHGGQYMGQLKMNETTHLVVQEPKGQKYECAKRWNVHCVTIQWFFDSIERGFCQDEVMYKTEPRPETKNLPDTSTPTGQTSKADSRTLSDVSHISNINSSCVNESAFTSALNSKLDSLLEDLENLDVSTLQAPEDLLDGCRIYLCGFSGRKLDKLRRLINCGGGVRFNQLNEDVTHVIIGDFDDELKHFWNKSTHRPYVVGAKWLLECFSKGYLLSEEPYVHVNYRAVEISVSEQPGIKPALSKRNSNLPKKDSVASEVQQQADEDLLSQYEKNDSTLGVAEKSEAGKFNDSTHILWKEEDQSSSHNHLPEASTIIESGLFSKKRFLVVGFSEDDEPCISDAIRENAGKVVSLQKRIIADYAVVPLLGCKVESTVGEVVTNTWLGMCIEHQVLFDPESNPLFTPLPVMEGKTPLEDCVLSFSQFTGAERDSLIYLASLLGARVQEFFVRKANTKKGMFASTHLVLKEPDGSKYEAAKKWNLPAITVSWLLQSARLGKKAEESKFLVENASAKAEESSQSQKPTDQTQVSGSDTPEKSSTLLESKRNSAVTPLDMNRFQSKAFHTVLSQQARKTSVSGEGKPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAVLETPRGCSQRHRKLSTPLSEVIGRNLKLAVANSTRHVAVLSASPRLEDAQLEKSIQKEEFKPLTDVVICVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRQNDSNREYRSVKERGIHIVSEHWLLQSAQEYRRLPESLYPHTYNPKMSLDISATQDERLYTGQLSSSDKIPKDNEPESLSMEVKNVEDGPSDPMEMETANDNHPNPKGVLTQTLEMRENFQKQLQEIMSATSIAKPQGQRGSLSRNGCDSTPSTPDSARSARSSRTRALEVLRQPHRTLTDLNTEPSQSEQIIWDDPTAREERARLASNLQWPTSPTRLSELPADVTDLDDSPFTDSLHDSETSELAAADPEKAWDTEDPKNQISEEAETTMKDDHLIPTPQAPSIAFPLANPPVAPQPKEKTVREKETREELTKQYIFQLSSLNPQERIDYCHLIEELGGSVLEKQCFDASCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRTAGCFVQEEDYEWGSSSILDVLTGINLQQKKLALAAMRWRKKIQRRQEEAGVVEGAFSGWRVVLNVDQSREAGFKRLLQSGGAKVLPGHSPSLFKEATHLFADFSKLKPDDTRVNVMEAAAQNVNCLKPEYIADYLMQELPPPMENYCLPGAAVPQNRKELDAGLSQKRKAPAEKNKVKRLRLH

Target ORF information:

RefSeq Version	XM_029070875.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029070875.1

ORF Insert Sequence:

ATGTCCAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGC TTGGTTGTAG AATTGTGGGA 
CCTCAAGTAG TCCTATTTTG TATGCAGCAC CAGCGATGTG TACCAAGAGC AGACTATCCG 
GTTTTTAATA TGATTATGGC AGATGTAACT ATATCCTGTA CCAGTCTTGA CAAAGAAACA 
AGGGAAGAAG TTCATAAATA TGTTCAGATG ATGGGTGGAC GAGTGTACAG AGATCTTAAT 
GTATCAGTAA CTCATCTTAT TGCTGGAGAA GTAGGCAGCA AAAAATACTT AGTAGCTGCT 
AGTTTAAAGA AACCTATCAT GCGCCCTTCT TGGATAAAAA CGCTATGGGA GAAGTCACAA 
GAAAACAGAA TGATCAGATG TACAGATGTA GACCTAGAAG AGTTTCGGTG CCCTCTATTT 
CTTGGCTGCA CAATTTGTGT GACTGGATTA AGTAGTCTGG ACAGGAAGGA GGTTCAGCGA 
CTAACGGTAG AACACGGCGG CCAATACATG GGACAATTAA AGATGAATGA AACCACTCAC 
CTTGTCGTGC AAGAACCAAA AGGGCAGAAA TATGAATGTG CCAAGAGATG GAATGTACAT 
TGTGTGACCA TCCAGTGGTT TTTCGACAGT ATTGAGAGAG GTTTTTGTCA AGACGAAGTT 
ATGTATAAGA CAGAGCCCAG ACCAGAAACA AAGAACCTGC CAGATACTTC AACTCCTACT 
GGTCAGACCA GCAAAGCCGA TAGTCGTACC CTTTCAGACG TCAGCCATAT TTCTAATATC 
AATTCAAGTT GCGTAAATGA GTCTGCGTTT ACCTCTGCTC TAAACAGCAA ATTGGATTCT 
CTCCTGGAGG ATTTAGAAAA TCTGGATGTC AGCACTTTAC AAGCACCAGA AGATTTACTA 
GACGGTTGTC GAATATATCT CTGTGGATTT AGTGGCCGAA AATTAGATAA GCTGAGAAGG 
CTTATTAATT GTGGTGGTGG AGTTCGTTTT AATCAGCTCA ACGAAGATGT CACTCATGTC 
ATTATAGGAG ACTTTGATGA TGAATTGAAG CACTTTTGGA ATAAGTCAAC ACACAGGCCA 
TATGTAGTAG GAGCAAAATG GCTGCTGGAG TGCTTTAGTA AGGGCTATCT GCTTTCTGAG 
GAGCCATATG TTCACGTGAA CTACCGAGCA GTGGAAATTT CCGTTTCAGA GCAACCTGGA 
ATCAAACCTG CCCTTTCAAA AAGAAATAGC AATTTGCCTA AGAAAGATTC CGTGGCCTCT 
GAGGTGCAGC AGCAAGCGGA TGAGGACCTA CTCTCTCAGT ATGAGAAAAA TGACTCGACA 
TTAGGGGTGG CTGAGAAATC TGAAGCTGGG AAATTTAATG ATTCTACTCA TATCCTTTGG 
AAAGAGGAGG ATCAATCTTC CAGTCACAAT CACCTTCCTG AGGCTTCTAC GATTATTGAA 
AGCGGATTGT TCAGCAAGAA AAGGTTCCTC GTTGTGGGTT TTTCTGAAGA TGATGAGCCT 
TGTATTTCAG ATGCTATAAG AGAAAATGCT GGGAAAGTTG TATCCCTCCA AAAAAGAATC 
ATTGCTGACT ATGCTGTGGT TCCTTTACTG GGGTGTAAAG TGGAGTCAAC TGTAGGAGAA 
GTGGTTACAA ACACATGGCT GGGTATGTGC ATTGAGCATC AGGTCCTGTT TGACCCAGAG 
TCAAATCCTC TTTTCACACC ACTACCTGTA ATGGAAGGGA AAACCCCTTT AGAAGACTGC 
GTTCTTTCAT TCAGCCAATT TACTGGGGCA GAGAGAGACT CCTTGATATA CTTAGCAAGT 
TTGCTTGGAG CGAGAGTTCA AGAGTTCTTC GTACGCAAAG CTAATACCAA AAAAGGCATG 
TTTGCCAGTA CCCACCTGGT GCTGAAAGAA CCTGATGGTT CCAAATATGA AGCGGCAAAA 
AAGTGGAATT TACCTGCAAT CACTGTGTCT TGGCTCTTAC AATCTGCGAG GCTGGGAAAG 
AAAGCTGAAG AAAGCAAGTT TCTGGTCGAG AACGCATCTG CCAAAGCAGA AGAAAGTTCT 
CAAAGTCAGA AACCAACAGA TCAGACTCAA GTATCTGGTT CAGACACACC AGAAAAGTCT 
AGCACACTCT TGGAGAGCAA AAGGAACTCA GCCGTGACAC CTTTGGATAT GAACAGGTTT 
CAGAGTAAAG CTTTTCATAC GGTACTCTCA CAGCAAGCCA GGAAAACCTC AGTTTCCGGA 
GAAGGAAAAC CACTTCAGAA GGAACCGTCG CTACACCTAG ACACACCATC GAAATTTCTG 
TCCAAAGACA AGCTCTTCAA GCCTTCATTT GATGTGAAGG ATGCCTTGGC AGTTTTGGAA 
ACTCCAAGAG GGTGTAGCCA GAGACACAGG AAGTTGAGCA CCCCCCTCTC AGAAGTCATT 
GGCAGGAACC TGAAACTGGC CGTAGCAAAT AGCACTCGTC ACGTAGCGGT TCTTTCCGCA 
AGTCCTCGGC TGGAGGATGC CCAGCTGGAA AAGTCCATAC AGAAAGAAGA ATTCAAACCA 
TTAACTGATG TAGTGATATG CGTCAGTAAG AAACTCAGTA AGAAGCAGAG CGAGCTGAAC 
GGTATTGCAG CATCTCTAGG AGCAGATTAC AGGTGGAGCT TCGATGAAAC GGTGACACAT 
TTCATCTACC AGGGGCGACA GAACGACAGT AATCGAGAAT ATAGATCTGT TAAAGAAAGA 
GGAATACATA TTGTTTCTGA ACACTGGCTT TTACAAAGTG CCCAAGAATA CAGACGCCTT 
CCCGAATCTC TTTACCCACA CACCTACAAT CCCAAAATGA GCCTGGATAT AAGTGCGACA 
CAAGATGAGA GGCTCTATAC CGGTCAGCTT TCATCATCTG ATAAAATACC AAAGGATAAT 
GAGCCTGAGT CTCTATCTAT GGAGGTGAAG AATGTAGAAG ACGGACCCTC TGATCCGATG 
GAGATGGAGA CCGCAAATGA CAATCACCCA AATCCCAAAG GAGTGCTAAC ACAGACCCTG 
GAAATGAGGG AAAACTTCCA GAAGCAGCTG CAAGAAATCA TGTCTGCGAC ATCAATAGCG 
AAACCCCAAG GGCAGAGGGG CTCCCTCTCC AGAAATGGGT GTGACAGCAC TCCTTCAACT 
CCAGACAGCG CGCGTTCTGC TCGAAGTAGT CGCACCCGAG CCTTGGAAGT GCTAAGACAG 
CCTCATCGGA CCCTAACCGA TCTCAACACC GAGCCCTCCC AGAGCGAACA GATCATTTGG 
GACGATCCTA CGGCACGAGA GGAAAGAGCG AGGCTGGCCA GCAACCTGCA GTGGCCCACC 
AGTCCCACGC GACTCTCCGA GCTCCCAGCC GACGTGACAG ACTTGGATGA CTCCCCGTTT 
ACGGACTCCT TACATGACTC TGAAACTTCC GAGCTAGCTG CTGCTGATCC CGAAAAAGCA 
TGGGACACTG AGGACCCGAA GAACCAGATC TCGGAAGAAG CAGAAACCAC AATGAAAGAT 
GACCACCTGA TCCCTACACC ACAAGCCCCC AGCATCGCTT TCCCCCTAGC CAACCCGCCC 
GTGGCTCCAC AACCCAAAGA GAAGACGGTA CGGGAAAAGG AGACTCGTGA AGAGCTGACA 
AAACAATACA TATTCCAGCT CTCCTCTCTC AATCCTCAAG AACGAATCGA CTACTGTCAT 
TTGATCGAGG AACTAGGTGG ATCGGTGCTT GAAAAGCAGT GCTTTGACGC GAGCTGCACG 
CACATCGTTG TGGGTCATCC TCTCCGAAAT GAGAAGTATT TGGCTTCCAT GGCAGCTGGC 
AAGTGGGTCC TTCATCGCTC CTACCTCGAG GCCTGTAGGA CCGCCGGGTG CTTTGTGCAG 
GAGGAAGATT ATGAATGGGG AAGCAGTTCC ATACTCGATG TGCTGACTGG AATCAACCTG 
CAGCAGAAAA AATTAGCCCT GGCAGCAATG AGATGGCGGA AGAAAATCCA GAGAAGACAA 
GAAGAAGCAG GCGTTGTTGA GGGGGCATTT AGTGGCTGGA GGGTAGTTTT AAATGTTGAC 
CAGTCCAGAG AAGCAGGATT CAAGCGTCTT CTTCAGTCAG GAGGGGCAAA GGTGCTGCCG 
GGGCACTCTC CATCTCTCTT TAAAGAAGCC ACACACCTCT TCGCGGACTT CAGTAAACTA 
AAACCAGACG ATACCAGAGT TAATGTGATG GAAGCTGCTG CCCAAAATGT GAACTGCTTG 
AAACCAGAAT ACATTGCAGA TTATCTTATG CAGGAGTTGC CTCCCCCGAT GGAGAATTAC 
TGCCTTCCTG GAGCAGCTGT TCCTCAGAAC AGAAAGGAAC TCGATGCAGG TCTATCCCAG 
AAGAGAAAAG CGCCCGCTGA AAAAAATAAA GTCAAGCGCC TCCGGCTACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPBP1 ( XM_029070876.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028926709.1 Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc133433
Clone ID Related Accession (Same CDS sequence)	XM_029070876.1
Accession Version	XM_029070876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041735.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGC TTGGTTGTAG AATTGTGGGA 
CCTCAAGTAG TCCTATTTTG TATGCAGCAC CAGCGATGTG TACCAAGAGC AGACTATCCG 
GTTTTTAATA TGATTATGGC AGATGTAACT ATATCCTGTA CCAGTCTTGA CAAAGAAACA 
AGGGAAGAAG TTCATAAATA TGTTCAGATG ATGGGTGGAC GAGTGTACAG AGATCTTAAT 
GTATCAGTAA CTCATCTTAT TGCTGGAGAA GTAGGCAGCA AAAAATACTT AGTAGCTGCT 
AGTTTAAAGA AACCTATCAT GCGCCCTTCT TGGATAAAAA CGCTATGGGA GAAGTCACAA 
GAAAAAATGA TCAGATGTAC AGATGTAGAC CTAGAAGAGT TTCGGTGCCC TCTATTTCTT 
GGCTGCACAA TTTGTGTGAC TGGATTAAGT AGTCTGGACA GGAAGGAGGT TCAGCGACTA 
ACGGTAGAAC ACGGCGGCCA ATACATGGGA CAATTAAAGA TGAATGAAAC CACTCACCTT 
GTCGTGCAAG AACCAAAAGG GCAGAAATAT GAATGTGCCA AGAGATGGAA TGTACATTGT 
GTGACCATCC AGTGGTTTTT CGACAGTATT GAGAGAGGTT TTTGTCAAGA CGAAGTTATG 
TATAAGACAG AGCCCAGACC AGAAACAAAG AACCTGCCAG ATACTTCAAC TCCTACTGGT 
CAGACCAGCA AAGCCGATAG TCGTACCCTT TCAGACGTCA GCCATATTTC TAATATCAAT 
TCAAGTTGCG TAAATGAGTC TGCGTTTACC TCTGCTCTAA ACAGCAAATT GGATTCTCTC 
CTGGAGGATT TAGAAAATCT GGATGTCAGC ACTTTACAAG CACCAGAAGA TTTACTAGAC 
GGTTGTCGAA TATATCTCTG TGGATTTAGT GGCCGAAAAT TAGATAAGCT GAGAAGGCTT 
ATTAATTGTG GTGGTGGAGT TCGTTTTAAT CAGCTCAACG AAGATGTCAC TCATGTCATT 
ATAGGAGACT TTGATGATGA ATTGAAGCAC TTTTGGAATA AGTCAACACA CAGGCCATAT 
GTAGTAGGAG CAAAATGGCT GCTGGAGTGC TTTAGTAAGG GCTATCTGCT TTCTGAGGAG 
CCATATGTTC ACGTGAACTA CCGAGCAGTG GAAATTTCCG TTTCAGAGCA ACCTGGAATC 
AAACCTGCCC TTTCAAAAAG AAATAGCAAT TTGCCTAAGA AAGATTCCGT GGCCTCTGAG 
GTGCAGCAGC AAGCGGATGA GGACCTACTC TCTCAGTATG AGAAAAATGA CTCGACATTA 
GGGGTGGCTG AGAAATCTGA AGCTGGGAAA TTTAATGATT CTACTCATAT CCTTTGGAAA 
GAGGAGGATC AATCTTCCAG TCACAATCAC CTTCCTGAGG CTTCTACGAT TATTGAAAGC 
GGATTGTTCA GCAAGAAAAG GTTCCTCGTT GTGGGTTTTT CTGAAGATGA TGAGCCTTGT 
ATTTCAGATG CTATAAGAGA AAATGCTGGG AAAGTTGTAT CCCTCCAAAA AAGAATCATT 
GCTGACTATG CTGTGGTTCC TTTACTGGGG TGTAAAGTGG AGTCAACTGT AGGAGAAGTG 
GTTACAAACA CATGGCTGGG TATGTGCATT GAGCATCAGG TCCTGTTTGA CCCAGAGTCA 
AATCCTCTTT TCACACCACT ACCTGTAATG GAAGGGAAAA CCCCTTTAGA AGACTGCGTT 
CTTTCATTCA GCCAATTTAC TGGGGCAGAG AGAGACTCCT TGATATACTT AGCAAGTTTG 
CTTGGAGCGA GAGTTCAAGA GTTCTTCGTA CGCAAAGCTA ATACCAAAAA AGGCATGTTT 
GCCAGTACCC ACCTGGTGCT GAAAGAACCT GATGGTTCCA AATATGAAGC GGCAAAAAAG 
TGGAATTTAC CTGCAATCAC TGTGTCTTGG CTCTTACAAT CTGCGAGGCT GGGAAAGAAA 
GCTGAAGAAA GCAAGTTTCT GGTCGAGAAC GCATCTGCCA AAGCAGAAGA AAGTTCTCAA 
AGTCAGAAAC CAACAGATCA GACTCAAGTA TCTGGTTCAG ACACACCAGA AAAGTCTAGC 
ACACTCTTGG AGAGCAAAAG GAACTCAGCC GTGACACCTT TGGATATGAA CAGGTTTCAG 
AGTAAAGCTT TTCATACGGT ACTCTCACAG CAAGCCAGGA AAACCTCAGT TTCCGGAGAA 
GGAAAACCAC TTCAGAAGGA ACCGTCGCTA CACCTAGACA CACCATCGAA ATTTCTGTCC 
AAAGACAAGC TCTTCAAGCC TTCATTTGAT GTGAAGGATG CCTTGGCAGT TTTGGAAACT 
CCAAGAGGGT GTAGCCAGAG ACACAGGAAG TTGAGCACCC CCCTCTCAGA AGTCATTGGC 
AGGAACCTGA AACTGGCCGT AGCAAATAGC ACTCGTCACG TAGCGGTTCT TTCCGCAAGT 
CCTCGGCTGG AGGATGCCCA GCTGGAAAAG TCCATACAGA AAGAAGAATT CAAACCATTA 
ACTGATGTAG TGATATGCGT CAGTAAGAAA CTCAGTAAGA AGCAGAGCGA GCTGAACGGT 
ATTGCAGCAT CTCTAGGAGC AGATTACAGG TGGAGCTTCG ATGAAACGGT GACACATTTC 
ATCTACCAGG GGCGACAGAA CGACAGTAAT CGAGAATATA GATCTGTTAA AGAAAGAGGA 
ATACATATTG TTTCTGAACA CTGGCTTTTA CAAAGTGCCC AAGAATACAG ACGCCTTCCC 
GAATCTCTTT ACCCACACAC CTACAATCCC AAAATGAGCC TGGATATAAG TGCGACACAA 
GATGAGAGGC TCTATACCGG TCAGCTTTCA TCATCTGATA AAATACCAAA GGATAATGAG 
CCTGAGTCTC TATCTATGGA GGTGAAGAAT GTAGAAGACG GACCCTCTGA TCCGATGGAG 
ATGGAGACCG CAAATGACAA TCACCCAAAT CCCAAAGGAG TGCTAACACA GACCCTGGAA 
ATGAGGGAAA ACTTCCAGAA GCAGCTGCAA GAAATCATGT CTGCGACATC AATAGCGAAA 
CCCCAAGGGC AGAGGGGCTC CCTCTCCAGA AATGGGTGTG ACAGCACTCC TTCAACTCCA 
GACAGCGCGC GTTCTGCTCG AAGTAGTCGC ACCCGAGCCT TGGAAGTGCT AAGACAGCCT 
CATCGGACCC TAACCGATCT CAACACCGAG CCCTCCCAGA GCGAACAGAT CATTTGGGAC 
GATCCTACGG CACGAGAGGA AAGAGCGAGG CTGGCCAGCA ACCTGCAGTG GCCCACCAGT 
CCCACGCGAC TCTCCGAGCT CCCAGCCGAC GTGACAGACT TGGATGACTC CCCGTTTACG 
GACTCCTTAC ATGACTCTGA AACTTCCGAG CTAGCTGCTG CTGATCCCGA AAAAGCATGG 
GACACTGAGG ACCCGAAGAA CCAGATCTCG GAAGAAGCAG AAACCACAAT GAAAGATGAC 
CACCTGATCC CTACACCACA AGCCCCCAGC ATCGCTTTCC CCCTAGCCAA CCCGCCCGTG 
GCTCCACAAC CCAAAGAGAA GACGGTACGG GAAAAGGAGA CTCGTGAAGA GCTGACAAAA 
CAATACATAT TCCAGCTCTC CTCTCTCAAT CCTCAAGAAC GAATCGACTA CTGTCATTTG 
ATCGAGGAAC TAGGTGGATC GGTGCTTGAA AAGCAGTGCT TTGACGCGAG CTGCACGCAC 
ATCGTTGTGG GTCATCCTCT CCGAAATGAG AAGTATTTGG CTTCCATGGC AGCTGGCAAG 
TGGGTCCTTC ATCGCTCCTA CCTCGAGGCC TGTAGGACCG CCGGGTGCTT TGTGCAGGAG 
GAAGATTATG AATGGGGAAG CAGTTCCATA CTCGATGTGC TGACTGGAAT CAACCTGCAG 
CAGAAAAAAT TAGCCCTGGC AGCAATGAGA TGGCGGAAGA AAATCCAGAG AAGACAAGAA 
GAAGCAGGCG TTGTTGAGGG GGCATTTAGT GGCTGGAGGG TAGTTTTAAA TGTTGACCAG 
TCCAGAGAAG CAGGATTCAA GCGTCTTCTT CAGTCAGGAG GGGCAAAGGT GCTGCCGGGG 
CACTCTCCAT CTCTCTTTAA AGAAGCCACA CACCTCTTCG CGGACTTCAG TAAACTAAAA 
CCAGACGATA CCAGAGTTAA TGTGATGGAA GCTGCTGCCC AAAATGTGAA CTGCTTGAAA 
CCAGAATACA TTGCAGATTA TCTTATGCAG GAGTTGCCTC CCCCGATGGA GAATTACTGC 
CTTCCTGGAG CAGCTGTTCC TCAGAACAGA AAGGAACTCG ATGCAGGTCT ATCCCAGAAG 
AGAAAAGCGC CCGCTGAAAA AAATAAAGTC AAGCGCCTCC GGCTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028926709.1
CDS	163..4713
Translation	MSSSDREPFFVKFIKSQDNREFFFTALESIKEFQSEEYLQTITEEEALKIKENDRSLYICDPFGGDVFEHLKKLGCRIVGPQVVLFCMQHQRCVPRADYPVFNMIMADVTISCTSLDKETREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAASLKKPIMRPSWIKTLWEKSQEKMIRCTDVDLEEFRCPLFLGCTICVTGLSSLDRKEVQRLTVEHGGQYMGQLKMNETTHLVVQEPKGQKYECAKRWNVHCVTIQWFFDSIERGFCQDEVMYKTEPRPETKNLPDTSTPTGQTSKADSRTLSDVSHISNINSSCVNESAFTSALNSKLDSLLEDLENLDVSTLQAPEDLLDGCRIYLCGFSGRKLDKLRRLINCGGGVRFNQLNEDVTHVIIGDFDDELKHFWNKSTHRPYVVGAKWLLECFSKGYLLSEEPYVHVNYRAVEISVSEQPGIKPALSKRNSNLPKKDSVASEVQQQADEDLLSQYEKNDSTLGVAEKSEAGKFNDSTHILWKEEDQSSSHNHLPEASTIIESGLFSKKRFLVVGFSEDDEPCISDAIRENAGKVVSLQKRIIADYAVVPLLGCKVESTVGEVVTNTWLGMCIEHQVLFDPESNPLFTPLPVMEGKTPLEDCVLSFSQFTGAERDSLIYLASLLGARVQEFFVRKANTKKGMFASTHLVLKEPDGSKYEAAKKWNLPAITVSWLLQSARLGKKAEESKFLVENASAKAEESSQSQKPTDQTQVSGSDTPEKSSTLLESKRNSAVTPLDMNRFQSKAFHTVLSQQARKTSVSGEGKPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAVLETPRGCSQRHRKLSTPLSEVIGRNLKLAVANSTRHVAVLSASPRLEDAQLEKSIQKEEFKPLTDVVICVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRQNDSNREYRSVKERGIHIVSEHWLLQSAQEYRRLPESLYPHTYNPKMSLDISATQDERLYTGQLSSSDKIPKDNEPESLSMEVKNVEDGPSDPMEMETANDNHPNPKGVLTQTLEMRENFQKQLQEIMSATSIAKPQGQRGSLSRNGCDSTPSTPDSARSARSSRTRALEVLRQPHRTLTDLNTEPSQSEQIIWDDPTAREERARLASNLQWPTSPTRLSELPADVTDLDDSPFTDSLHDSETSELAAADPEKAWDTEDPKNQISEEAETTMKDDHLIPTPQAPSIAFPLANPPVAPQPKEKTVREKETREELTKQYIFQLSSLNPQERIDYCHLIEELGGSVLEKQCFDASCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRTAGCFVQEEDYEWGSSSILDVLTGINLQQKKLALAAMRWRKKIQRRQEEAGVVEGAFSGWRVVLNVDQSREAGFKRLLQSGGAKVLPGHSPSLFKEATHLFADFSKLKPDDTRVNVMEAAAQNVNCLKPEYIADYLMQELPPPMENYCLPGAAVPQNRKELDAGLSQKRKAPAEKNKVKRLRLH

Target ORF information:

RefSeq Version	XM_029070876.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029070876.1

ORF Insert Sequence:

ATGTCCAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGC TTGGTTGTAG AATTGTGGGA 
CCTCAAGTAG TCCTATTTTG TATGCAGCAC CAGCGATGTG TACCAAGAGC AGACTATCCG 
GTTTTTAATA TGATTATGGC AGATGTAACT ATATCCTGTA CCAGTCTTGA CAAAGAAACA 
AGGGAAGAAG TTCATAAATA TGTTCAGATG ATGGGTGGAC GAGTGTACAG AGATCTTAAT 
GTATCAGTAA CTCATCTTAT TGCTGGAGAA GTAGGCAGCA AAAAATACTT AGTAGCTGCT 
AGTTTAAAGA AACCTATCAT GCGCCCTTCT TGGATAAAAA CGCTATGGGA GAAGTCACAA 
GAAAAAATGA TCAGATGTAC AGATGTAGAC CTAGAAGAGT TTCGGTGCCC TCTATTTCTT 
GGCTGCACAA TTTGTGTGAC TGGATTAAGT AGTCTGGACA GGAAGGAGGT TCAGCGACTA 
ACGGTAGAAC ACGGCGGCCA ATACATGGGA CAATTAAAGA TGAATGAAAC CACTCACCTT 
GTCGTGCAAG AACCAAAAGG GCAGAAATAT GAATGTGCCA AGAGATGGAA TGTACATTGT 
GTGACCATCC AGTGGTTTTT CGACAGTATT GAGAGAGGTT TTTGTCAAGA CGAAGTTATG 
TATAAGACAG AGCCCAGACC AGAAACAAAG AACCTGCCAG ATACTTCAAC TCCTACTGGT 
CAGACCAGCA AAGCCGATAG TCGTACCCTT TCAGACGTCA GCCATATTTC TAATATCAAT 
TCAAGTTGCG TAAATGAGTC TGCGTTTACC TCTGCTCTAA ACAGCAAATT GGATTCTCTC 
CTGGAGGATT TAGAAAATCT GGATGTCAGC ACTTTACAAG CACCAGAAGA TTTACTAGAC 
GGTTGTCGAA TATATCTCTG TGGATTTAGT GGCCGAAAAT TAGATAAGCT GAGAAGGCTT 
ATTAATTGTG GTGGTGGAGT TCGTTTTAAT CAGCTCAACG AAGATGTCAC TCATGTCATT 
ATAGGAGACT TTGATGATGA ATTGAAGCAC TTTTGGAATA AGTCAACACA CAGGCCATAT 
GTAGTAGGAG CAAAATGGCT GCTGGAGTGC TTTAGTAAGG GCTATCTGCT TTCTGAGGAG 
CCATATGTTC ACGTGAACTA CCGAGCAGTG GAAATTTCCG TTTCAGAGCA ACCTGGAATC 
AAACCTGCCC TTTCAAAAAG AAATAGCAAT TTGCCTAAGA AAGATTCCGT GGCCTCTGAG 
GTGCAGCAGC AAGCGGATGA GGACCTACTC TCTCAGTATG AGAAAAATGA CTCGACATTA 
GGGGTGGCTG AGAAATCTGA AGCTGGGAAA TTTAATGATT CTACTCATAT CCTTTGGAAA 
GAGGAGGATC AATCTTCCAG TCACAATCAC CTTCCTGAGG CTTCTACGAT TATTGAAAGC 
GGATTGTTCA GCAAGAAAAG GTTCCTCGTT GTGGGTTTTT CTGAAGATGA TGAGCCTTGT 
ATTTCAGATG CTATAAGAGA AAATGCTGGG AAAGTTGTAT CCCTCCAAAA AAGAATCATT 
GCTGACTATG CTGTGGTTCC TTTACTGGGG TGTAAAGTGG AGTCAACTGT AGGAGAAGTG 
GTTACAAACA CATGGCTGGG TATGTGCATT GAGCATCAGG TCCTGTTTGA CCCAGAGTCA 
AATCCTCTTT TCACACCACT ACCTGTAATG GAAGGGAAAA CCCCTTTAGA AGACTGCGTT 
CTTTCATTCA GCCAATTTAC TGGGGCAGAG AGAGACTCCT TGATATACTT AGCAAGTTTG 
CTTGGAGCGA GAGTTCAAGA GTTCTTCGTA CGCAAAGCTA ATACCAAAAA AGGCATGTTT 
GCCAGTACCC ACCTGGTGCT GAAAGAACCT GATGGTTCCA AATATGAAGC GGCAAAAAAG 
TGGAATTTAC CTGCAATCAC TGTGTCTTGG CTCTTACAAT CTGCGAGGCT GGGAAAGAAA 
GCTGAAGAAA GCAAGTTTCT GGTCGAGAAC GCATCTGCCA AAGCAGAAGA AAGTTCTCAA 
AGTCAGAAAC CAACAGATCA GACTCAAGTA TCTGGTTCAG ACACACCAGA AAAGTCTAGC 
ACACTCTTGG AGAGCAAAAG GAACTCAGCC GTGACACCTT TGGATATGAA CAGGTTTCAG 
AGTAAAGCTT TTCATACGGT ACTCTCACAG CAAGCCAGGA AAACCTCAGT TTCCGGAGAA 
GGAAAACCAC TTCAGAAGGA ACCGTCGCTA CACCTAGACA CACCATCGAA ATTTCTGTCC 
AAAGACAAGC TCTTCAAGCC TTCATTTGAT GTGAAGGATG CCTTGGCAGT TTTGGAAACT 
CCAAGAGGGT GTAGCCAGAG ACACAGGAAG TTGAGCACCC CCCTCTCAGA AGTCATTGGC 
AGGAACCTGA AACTGGCCGT AGCAAATAGC ACTCGTCACG TAGCGGTTCT TTCCGCAAGT 
CCTCGGCTGG AGGATGCCCA GCTGGAAAAG TCCATACAGA AAGAAGAATT CAAACCATTA 
ACTGATGTAG TGATATGCGT CAGTAAGAAA CTCAGTAAGA AGCAGAGCGA GCTGAACGGT 
ATTGCAGCAT CTCTAGGAGC AGATTACAGG TGGAGCTTCG ATGAAACGGT GACACATTTC 
ATCTACCAGG GGCGACAGAA CGACAGTAAT CGAGAATATA GATCTGTTAA AGAAAGAGGA 
ATACATATTG TTTCTGAACA CTGGCTTTTA CAAAGTGCCC AAGAATACAG ACGCCTTCCC 
GAATCTCTTT ACCCACACAC CTACAATCCC AAAATGAGCC TGGATATAAG TGCGACACAA 
GATGAGAGGC TCTATACCGG TCAGCTTTCA TCATCTGATA AAATACCAAA GGATAATGAG 
CCTGAGTCTC TATCTATGGA GGTGAAGAAT GTAGAAGACG GACCCTCTGA TCCGATGGAG 
ATGGAGACCG CAAATGACAA TCACCCAAAT CCCAAAGGAG TGCTAACACA GACCCTGGAA 
ATGAGGGAAA ACTTCCAGAA GCAGCTGCAA GAAATCATGT CTGCGACATC AATAGCGAAA 
CCCCAAGGGC AGAGGGGCTC CCTCTCCAGA AATGGGTGTG ACAGCACTCC TTCAACTCCA 
GACAGCGCGC GTTCTGCTCG AAGTAGTCGC ACCCGAGCCT TGGAAGTGCT AAGACAGCCT 
CATCGGACCC TAACCGATCT CAACACCGAG CCCTCCCAGA GCGAACAGAT CATTTGGGAC 
GATCCTACGG CACGAGAGGA AAGAGCGAGG CTGGCCAGCA ACCTGCAGTG GCCCACCAGT 
CCCACGCGAC TCTCCGAGCT CCCAGCCGAC GTGACAGACT TGGATGACTC CCCGTTTACG 
GACTCCTTAC ATGACTCTGA AACTTCCGAG CTAGCTGCTG CTGATCCCGA AAAAGCATGG 
GACACTGAGG ACCCGAAGAA CCAGATCTCG GAAGAAGCAG AAACCACAAT GAAAGATGAC 
CACCTGATCC CTACACCACA AGCCCCCAGC ATCGCTTTCC CCCTAGCCAA CCCGCCCGTG 
GCTCCACAAC CCAAAGAGAA GACGGTACGG GAAAAGGAGA CTCGTGAAGA GCTGACAAAA 
CAATACATAT TCCAGCTCTC CTCTCTCAAT CCTCAAGAAC GAATCGACTA CTGTCATTTG 
ATCGAGGAAC TAGGTGGATC GGTGCTTGAA AAGCAGTGCT TTGACGCGAG CTGCACGCAC 
ATCGTTGTGG GTCATCCTCT CCGAAATGAG AAGTATTTGG CTTCCATGGC AGCTGGCAAG 
TGGGTCCTTC ATCGCTCCTA CCTCGAGGCC TGTAGGACCG CCGGGTGCTT TGTGCAGGAG 
GAAGATTATG AATGGGGAAG CAGTTCCATA CTCGATGTGC TGACTGGAAT CAACCTGCAG 
CAGAAAAAAT TAGCCCTGGC AGCAATGAGA TGGCGGAAGA AAATCCAGAG AAGACAAGAA 
GAAGCAGGCG TTGTTGAGGG GGCATTTAGT GGCTGGAGGG TAGTTTTAAA TGTTGACCAG 
TCCAGAGAAG CAGGATTCAA GCGTCTTCTT CAGTCAGGAG GGGCAAAGGT GCTGCCGGGG 
CACTCTCCAT CTCTCTTTAA AGAAGCCACA CACCTCTTCG CGGACTTCAG TAAACTAAAA 
CCAGACGATA CCAGAGTTAA TGTGATGGAA GCTGCTGCCC AAAATGTGAA CTGCTTGAAA 
CCAGAATACA TTGCAGATTA TCTTATGCAG GAGTTGCCTC CCCCGATGGA GAATTACTGC 
CTTCCTGGAG CAGCTGTTCC TCAGAACAGA AAGGAACTCG ATGCAGGTCT ATCCCAGAAG 
AGAAAAGCGC CCGCTGAAAA AAATAAAGTC AAGCGCCTCC GGCTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPBP1 ( XM_029070877.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028926710.1 Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA.

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CloneID	OOc133434
Clone ID Related Accession (Same CDS sequence)	XM_029070877.1
Accession Version	XM_029070877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	DNA topoisomerase 2-binding protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041735.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGC TTGGTTGTAG AATTGTGGGA 
CCTCAAGTAG TCCTATTTTG TATGCAGCAC CAGCGATGTG TACCAAGAGC AGACTATCCG 
GTTTTTAATA TGATTATGGC AGATGTAACT ATATCCTGTA CCAGTCTTGA CAAAGAAACA 
AGGGAAGAAG TTCATAAATA TGTTCAGATG ATGGGTGGAC GAGTGTACAG AGATCTTAAT 
GTATCAGTAA CTCATCTTAT TGCTGGAGAA GTAGGCAGCA AAAAATACTT AGTAGCTGCT 
AGTTTAAAGA AACCTATCAT GCGCCCTTCT TGGATAAAAA CGCTATGGGA GAAGTCACAA 
GAAAACAGAA TGATCAGATG TACAGATGTA GACCTAGAAG AGTTTCGGTG CCCTCTATTT 
CTTGGCTGCA CAATTTGTGT GACTGGATTA AGTAGTCTGG ACAGGAAGGA GGTTCAGCGA 
CTAACGGTAG AACACGGCGG CCAATACATG GGACAATTAA AGATGAATGA AACCACTCAC 
CTTGTCGTGC AAGAACCAAA AGGGCAGAAA TATGAATGTG CCAAGAGATG GAATGTACAT 
TGTGTGACCA TCCAGTGGTT TTTCGACAGT ATTGAGAGAG GTTTTTGTCA AGACGAAGTT 
ATGTATAAGA CAGAGCCCAG ACCAGAAACA AAGAACCTGC CAGATACTTC AACTCCTACT 
GGTCAGACCA GCAAAGCCGA TAGTCGTACC CTTTCAGACG TCAGCCATAT TTCTAATATC 
AATTCAAGTT GCGTAAATGA GTCTGCGTTT ACCTCTGCTC TAAACAGCAA ATTGGATTCT 
CTCCTGGAGG ATTTAGAAAA TCTGGATGTC AGCACTTTAC AAGCACCAGA AGATTTACTA 
GACGGTTGTC GAATATATCT CTGTGGATTT AGTGGCCGAA AATTAGATAA GCTGAGAAGG 
CTTATTAATT GTGGTGGTGG AGTTCGTTTT AATCAGCTCA ACGAAGATGT CACTCATGTC 
ATTATAGGAG ACTTTGATGA TGAATTGAAG CACTTTTGGA ATAAGTCAAC ACACAGGCCA 
TATGTAGTAG GAGCAAAATG GCTGCTGGAG TGCTTTAGTA AGGGCTATCT GCTTTCTGAG 
GAGCCATATG TTCACGTGAA CTACCGAGCA GTGGAAATTT CCGTTTCAGA GCAACCTGGA 
ATCAAACCTG CCCTTTCAAA AAGAAATAGC AATTTGCCTA AGAAAGATTC CGTGGCCTCT 
GAGGTGCAGC AGCAAGCGGA TGAGGACCTA CTCTCTCAGT ATGAGAAAAA TGACTCGACA 
TTAGGGGTGG CTGAGAAATC TGAAGCTGGG AAATTTAATG ATTCTACTCA TATCCTTTGG 
AAAGAGGAGG ATCAATCTTC CAGTCACAAT CACCTTCCTG AGGCTTCTAC GATTATTGAA 
AGCGGATTGT TCAGCAAGAA AAGGTTCCTC GTTGTGGGTT TTTCTGAAGA TGATGAGCCT 
TGTATTTCAG ATGCTATAAG AGAAAATGCT GGGAAAGTTG TATCCCTCCA AAAAAGAATC 
ATTGCTGACT ATGCTGTGGT TCCTTTACTG GGGTGTAAAG TGGAGTCAAC TGTAGGAGAA 
GTGGTTACAA ACACATGGCT GGGTATGTGC ATTGAGCATC AGGTCCTGTT TGACCCAGAG 
TCAAATCCTC TTTTCACACC ACTACCTGTA ATGGAAGGGA AAACCCCTTT AGAAGACTGC 
GTTCTTTCAT TCAGCCAATT TACTGGGGCA GAGAGAGACT CCTTGATATA CTTAGCAAGT 
TTGCTTGGAG CGAGAGTTCA AGAGTTCTTC GTACGCAAAG CTAATACCAA AAAAGGCATG 
TTTGCCAGTA CCCACCTGGT GCTGAAAGAA CCTGATGGTT CCAAATATGA AGCGGCAAAA 
AAGTGGAATT TACCTGCAAT CACTGTGTCT TGGCTCTTAC AATCTGCGAG GCTGGGAAAG 
AAAGCTGAAG AAAGCAAGTT TCTGGTCGAG AACGCATCTG CCAAAGAAGA AAGTTCTCAA 
AGTCAGAAAC CAACAGATCA GACTCAAGTA TCTGGTTCAG ACACACCAGA AAAGTCTAGC 
ACACTCTTGG AGAGCAAAAG GAACTCAGCC GTGACACCTT TGGATATGAA CAGGTTTCAG 
AGTAAAGCTT TTCATACGGT ACTCTCACAG CAAGCCAGGA AAACCTCAGT TTCCGGAGAA 
GGAAAACCAC TTCAGAAGGA ACCGTCGCTA CACCTAGACA CACCATCGAA ATTTCTGTCC 
AAAGACAAGC TCTTCAAGCC TTCATTTGAT GTGAAGGATG CCTTGGCAGT TTTGGAAACT 
CCAAGAGGGT GTAGCCAGAG ACACAGGAAG TTGAGCACCC CCCTCTCAGA AGTCATTGGC 
AGGAACCTGA AACTGGCCGT AGCAAATAGC ACTCGTCACG TAGCGGTTCT TTCCGCAAGT 
CCTCGGCTGG AGGATGCCCA GCTGGAAAAG TCCATACAGA AAGAAGAATT CAAACCATTA 
ACTGATGTAG TGATATGCGT CAGTAAGAAA CTCAGTAAGA AGCAGAGCGA GCTGAACGGT 
ATTGCAGCAT CTCTAGGAGC AGATTACAGG TGGAGCTTCG ATGAAACGGT GACACATTTC 
ATCTACCAGG GGCGACAGAA CGACAGTAAT CGAGAATATA GATCTGTTAA AGAAAGAGGA 
ATACATATTG TTTCTGAACA CTGGCTTTTA CAAAGTGCCC AAGAATACAG ACGCCTTCCC 
GAATCTCTTT ACCCACACAC CTACAATCCC AAAATGAGCC TGGATATAAG TGCGACACAA 
GATGAGAGGC TCTATACCGG TCAGCTTTCA TCATCTGATA AAATACCAAA GGATAATGAG 
CCTGAGTCTC TATCTATGGA GGTGAAGAAT GTAGAAGACG GACCCTCTGA TCCGATGGAG 
ATGGAGACCG CAAATGACAA TCACCCAAAT CCCAAAGGAG TGCTAACACA GACCCTGGAA 
ATGAGGGAAA ACTTCCAGAA GCAGCTGCAA GAAATCATGT CTGCGACATC AATAGCGAAA 
CCCCAAGGGC AGAGGGGCTC CCTCTCCAGA AATGGGTGTG ACAGCACTCC TTCAACTCCA 
GACAGCGCGC GTTCTGCTCG AAGTAGTCGC ACCCGAGCCT TGGAAGTGCT AAGACAGCCT 
CATCGGACCC TAACCGATCT CAACACCGAG CCCTCCCAGA GCGAACAGAT CATTTGGGAC 
GATCCTACGG CACGAGAGGA AAGAGCGAGG CTGGCCAGCA ACCTGCAGTG GCCCACCAGT 
CCCACGCGAC TCTCCGAGCT CCCAGCCGAC GTGACAGACT TGGATGACTC CCCGTTTACG 
GACTCCTTAC ATGACTCTGA AACTTCCGAG CTAGCTGCTG CTGATCCCGA AAAAGCATGG 
GACACTGAGG ACCCGAAGAA CCAGATCTCG GAAGAAGCAG AAACCACAAT GAAAGATGAC 
CACCTGATCC CTACACCACA AGCCCCCAGC ATCGCTTTCC CCCTAGCCAA CCCGCCCGTG 
GCTCCACAAC CCAAAGAGAA GACGGTACGG GAAAAGGAGA CTCGTGAAGA GCTGACAAAA 
CAATACATAT TCCAGCTCTC CTCTCTCAAT CCTCAAGAAC GAATCGACTA CTGTCATTTG 
ATCGAGGAAC TAGGTGGATC GGTGCTTGAA AAGCAGTGCT TTGACGCGAG CTGCACGCAC 
ATCGTTGTGG GTCATCCTCT CCGAAATGAG AAGTATTTGG CTTCCATGGC AGCTGGCAAG 
TGGGTCCTTC ATCGCTCCTA CCTCGAGGCC TGTAGGACCG CCGGGTGCTT TGTGCAGGAG 
GAAGATTATG AATGGGGAAG CAGTTCCATA CTCGATGTGC TGACTGGAAT CAACCTGCAG 
CAGAAAAAAT TAGCCCTGGC AGCAATGAGA TGGCGGAAGA AAATCCAGAG AAGACAAGAA 
GAAGCAGGCG TTGTTGAGGG GGCATTTAGT GGCTGGAGGG TAGTTTTAAA TGTTGACCAG 
TCCAGAGAAG CAGGATTCAA GCGTCTTCTT CAGTCAGGAG GGGCAAAGGT GCTGCCGGGG 
CACTCTCCAT CTCTCTTTAA AGAAGCCACA CACCTCTTCG CGGACTTCAG TAAACTAAAA 
CCAGACGATA CCAGAGTTAA TGTGATGGAA GCTGCTGCCC AAAATGTGAA CTGCTTGAAA 
CCAGAATACA TTGCAGATTA TCTTATGCAG GAGTTGCCTC CCCCGATGGA GAATTACTGC 
CTTCCTGGAG CAGCTGTTCC TCAGAACAGA AAGGAACTCG ATGCAGGTCT ATCCCAGAAG 
AGAAAAGCGC CCGCTGAAAA AAATAAAGTC AAGCGCCTCC GGCTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028926710.1
CDS	164..4714
Translation	MSSSDREPFFVKFIKSQDNREFFFTALESIKEFQSEEYLQTITEEEALKIKENDRSLYICDPFGGDVFEHLKKLGCRIVGPQVVLFCMQHQRCVPRADYPVFNMIMADVTISCTSLDKETREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAASLKKPIMRPSWIKTLWEKSQENRMIRCTDVDLEEFRCPLFLGCTICVTGLSSLDRKEVQRLTVEHGGQYMGQLKMNETTHLVVQEPKGQKYECAKRWNVHCVTIQWFFDSIERGFCQDEVMYKTEPRPETKNLPDTSTPTGQTSKADSRTLSDVSHISNINSSCVNESAFTSALNSKLDSLLEDLENLDVSTLQAPEDLLDGCRIYLCGFSGRKLDKLRRLINCGGGVRFNQLNEDVTHVIIGDFDDELKHFWNKSTHRPYVVGAKWLLECFSKGYLLSEEPYVHVNYRAVEISVSEQPGIKPALSKRNSNLPKKDSVASEVQQQADEDLLSQYEKNDSTLGVAEKSEAGKFNDSTHILWKEEDQSSSHNHLPEASTIIESGLFSKKRFLVVGFSEDDEPCISDAIRENAGKVVSLQKRIIADYAVVPLLGCKVESTVGEVVTNTWLGMCIEHQVLFDPESNPLFTPLPVMEGKTPLEDCVLSFSQFTGAERDSLIYLASLLGARVQEFFVRKANTKKGMFASTHLVLKEPDGSKYEAAKKWNLPAITVSWLLQSARLGKKAEESKFLVENASAKEESSQSQKPTDQTQVSGSDTPEKSSTLLESKRNSAVTPLDMNRFQSKAFHTVLSQQARKTSVSGEGKPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAVLETPRGCSQRHRKLSTPLSEVIGRNLKLAVANSTRHVAVLSASPRLEDAQLEKSIQKEEFKPLTDVVICVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRQNDSNREYRSVKERGIHIVSEHWLLQSAQEYRRLPESLYPHTYNPKMSLDISATQDERLYTGQLSSSDKIPKDNEPESLSMEVKNVEDGPSDPMEMETANDNHPNPKGVLTQTLEMRENFQKQLQEIMSATSIAKPQGQRGSLSRNGCDSTPSTPDSARSARSSRTRALEVLRQPHRTLTDLNTEPSQSEQIIWDDPTAREERARLASNLQWPTSPTRLSELPADVTDLDDSPFTDSLHDSETSELAAADPEKAWDTEDPKNQISEEAETTMKDDHLIPTPQAPSIAFPLANPPVAPQPKEKTVREKETREELTKQYIFQLSSLNPQERIDYCHLIEELGGSVLEKQCFDASCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRTAGCFVQEEDYEWGSSSILDVLTGINLQQKKLALAAMRWRKKIQRRQEEAGVVEGAFSGWRVVLNVDQSREAGFKRLLQSGGAKVLPGHSPSLFKEATHLFADFSKLKPDDTRVNVMEAAAQNVNCLKPEYIADYLMQELPPPMENYCLPGAAVPQNRKELDAGLSQKRKAPAEKNKVKRLRLH

Target ORF information:

RefSeq Version	XM_029070877.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029070877.1

ORF Insert Sequence:

ATGTCCAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGC TTGGTTGTAG AATTGTGGGA 
CCTCAAGTAG TCCTATTTTG TATGCAGCAC CAGCGATGTG TACCAAGAGC AGACTATCCG 
GTTTTTAATA TGATTATGGC AGATGTAACT ATATCCTGTA CCAGTCTTGA CAAAGAAACA 
AGGGAAGAAG TTCATAAATA TGTTCAGATG ATGGGTGGAC GAGTGTACAG AGATCTTAAT 
GTATCAGTAA CTCATCTTAT TGCTGGAGAA GTAGGCAGCA AAAAATACTT AGTAGCTGCT 
AGTTTAAAGA AACCTATCAT GCGCCCTTCT TGGATAAAAA CGCTATGGGA GAAGTCACAA 
GAAAACAGAA TGATCAGATG TACAGATGTA GACCTAGAAG AGTTTCGGTG CCCTCTATTT 
CTTGGCTGCA CAATTTGTGT GACTGGATTA AGTAGTCTGG ACAGGAAGGA GGTTCAGCGA 
CTAACGGTAG AACACGGCGG CCAATACATG GGACAATTAA AGATGAATGA AACCACTCAC 
CTTGTCGTGC AAGAACCAAA AGGGCAGAAA TATGAATGTG CCAAGAGATG GAATGTACAT 
TGTGTGACCA TCCAGTGGTT TTTCGACAGT ATTGAGAGAG GTTTTTGTCA AGACGAAGTT 
ATGTATAAGA CAGAGCCCAG ACCAGAAACA AAGAACCTGC CAGATACTTC AACTCCTACT 
GGTCAGACCA GCAAAGCCGA TAGTCGTACC CTTTCAGACG TCAGCCATAT TTCTAATATC 
AATTCAAGTT GCGTAAATGA GTCTGCGTTT ACCTCTGCTC TAAACAGCAA ATTGGATTCT 
CTCCTGGAGG ATTTAGAAAA TCTGGATGTC AGCACTTTAC AAGCACCAGA AGATTTACTA 
GACGGTTGTC GAATATATCT CTGTGGATTT AGTGGCCGAA AATTAGATAA GCTGAGAAGG 
CTTATTAATT GTGGTGGTGG AGTTCGTTTT AATCAGCTCA ACGAAGATGT CACTCATGTC 
ATTATAGGAG ACTTTGATGA TGAATTGAAG CACTTTTGGA ATAAGTCAAC ACACAGGCCA 
TATGTAGTAG GAGCAAAATG GCTGCTGGAG TGCTTTAGTA AGGGCTATCT GCTTTCTGAG 
GAGCCATATG TTCACGTGAA CTACCGAGCA GTGGAAATTT CCGTTTCAGA GCAACCTGGA 
ATCAAACCTG CCCTTTCAAA AAGAAATAGC AATTTGCCTA AGAAAGATTC CGTGGCCTCT 
GAGGTGCAGC AGCAAGCGGA TGAGGACCTA CTCTCTCAGT ATGAGAAAAA TGACTCGACA 
TTAGGGGTGG CTGAGAAATC TGAAGCTGGG AAATTTAATG ATTCTACTCA TATCCTTTGG 
AAAGAGGAGG ATCAATCTTC CAGTCACAAT CACCTTCCTG AGGCTTCTAC GATTATTGAA 
AGCGGATTGT TCAGCAAGAA AAGGTTCCTC GTTGTGGGTT TTTCTGAAGA TGATGAGCCT 
TGTATTTCAG ATGCTATAAG AGAAAATGCT GGGAAAGTTG TATCCCTCCA AAAAAGAATC 
ATTGCTGACT ATGCTGTGGT TCCTTTACTG GGGTGTAAAG TGGAGTCAAC TGTAGGAGAA 
GTGGTTACAA ACACATGGCT GGGTATGTGC ATTGAGCATC AGGTCCTGTT TGACCCAGAG 
TCAAATCCTC TTTTCACACC ACTACCTGTA ATGGAAGGGA AAACCCCTTT AGAAGACTGC 
GTTCTTTCAT TCAGCCAATT TACTGGGGCA GAGAGAGACT CCTTGATATA CTTAGCAAGT 
TTGCTTGGAG CGAGAGTTCA AGAGTTCTTC GTACGCAAAG CTAATACCAA AAAAGGCATG 
TTTGCCAGTA CCCACCTGGT GCTGAAAGAA CCTGATGGTT CCAAATATGA AGCGGCAAAA 
AAGTGGAATT TACCTGCAAT CACTGTGTCT TGGCTCTTAC AATCTGCGAG GCTGGGAAAG 
AAAGCTGAAG AAAGCAAGTT TCTGGTCGAG AACGCATCTG CCAAAGAAGA AAGTTCTCAA 
AGTCAGAAAC CAACAGATCA GACTCAAGTA TCTGGTTCAG ACACACCAGA AAAGTCTAGC 
ACACTCTTGG AGAGCAAAAG GAACTCAGCC GTGACACCTT TGGATATGAA CAGGTTTCAG 
AGTAAAGCTT TTCATACGGT ACTCTCACAG CAAGCCAGGA AAACCTCAGT TTCCGGAGAA 
GGAAAACCAC TTCAGAAGGA ACCGTCGCTA CACCTAGACA CACCATCGAA ATTTCTGTCC 
AAAGACAAGC TCTTCAAGCC TTCATTTGAT GTGAAGGATG CCTTGGCAGT TTTGGAAACT 
CCAAGAGGGT GTAGCCAGAG ACACAGGAAG TTGAGCACCC CCCTCTCAGA AGTCATTGGC 
AGGAACCTGA AACTGGCCGT AGCAAATAGC ACTCGTCACG TAGCGGTTCT TTCCGCAAGT 
CCTCGGCTGG AGGATGCCCA GCTGGAAAAG TCCATACAGA AAGAAGAATT CAAACCATTA 
ACTGATGTAG TGATATGCGT CAGTAAGAAA CTCAGTAAGA AGCAGAGCGA GCTGAACGGT 
ATTGCAGCAT CTCTAGGAGC AGATTACAGG TGGAGCTTCG ATGAAACGGT GACACATTTC 
ATCTACCAGG GGCGACAGAA CGACAGTAAT CGAGAATATA GATCTGTTAA AGAAAGAGGA 
ATACATATTG TTTCTGAACA CTGGCTTTTA CAAAGTGCCC AAGAATACAG ACGCCTTCCC 
GAATCTCTTT ACCCACACAC CTACAATCCC AAAATGAGCC TGGATATAAG TGCGACACAA 
GATGAGAGGC TCTATACCGG TCAGCTTTCA TCATCTGATA AAATACCAAA GGATAATGAG 
CCTGAGTCTC TATCTATGGA GGTGAAGAAT GTAGAAGACG GACCCTCTGA TCCGATGGAG 
ATGGAGACCG CAAATGACAA TCACCCAAAT CCCAAAGGAG TGCTAACACA GACCCTGGAA 
ATGAGGGAAA ACTTCCAGAA GCAGCTGCAA GAAATCATGT CTGCGACATC AATAGCGAAA 
CCCCAAGGGC AGAGGGGCTC CCTCTCCAGA AATGGGTGTG ACAGCACTCC TTCAACTCCA 
GACAGCGCGC GTTCTGCTCG AAGTAGTCGC ACCCGAGCCT TGGAAGTGCT AAGACAGCCT 
CATCGGACCC TAACCGATCT CAACACCGAG CCCTCCCAGA GCGAACAGAT CATTTGGGAC 
GATCCTACGG CACGAGAGGA AAGAGCGAGG CTGGCCAGCA ACCTGCAGTG GCCCACCAGT 
CCCACGCGAC TCTCCGAGCT CCCAGCCGAC GTGACAGACT TGGATGACTC CCCGTTTACG 
GACTCCTTAC ATGACTCTGA AACTTCCGAG CTAGCTGCTG CTGATCCCGA AAAAGCATGG 
GACACTGAGG ACCCGAAGAA CCAGATCTCG GAAGAAGCAG AAACCACAAT GAAAGATGAC 
CACCTGATCC CTACACCACA AGCCCCCAGC ATCGCTTTCC CCCTAGCCAA CCCGCCCGTG 
GCTCCACAAC CCAAAGAGAA GACGGTACGG GAAAAGGAGA CTCGTGAAGA GCTGACAAAA 
CAATACATAT TCCAGCTCTC CTCTCTCAAT CCTCAAGAAC GAATCGACTA CTGTCATTTG 
ATCGAGGAAC TAGGTGGATC GGTGCTTGAA AAGCAGTGCT TTGACGCGAG CTGCACGCAC 
ATCGTTGTGG GTCATCCTCT CCGAAATGAG AAGTATTTGG CTTCCATGGC AGCTGGCAAG 
TGGGTCCTTC ATCGCTCCTA CCTCGAGGCC TGTAGGACCG CCGGGTGCTT TGTGCAGGAG 
GAAGATTATG AATGGGGAAG CAGTTCCATA CTCGATGTGC TGACTGGAAT CAACCTGCAG 
CAGAAAAAAT TAGCCCTGGC AGCAATGAGA TGGCGGAAGA AAATCCAGAG AAGACAAGAA 
GAAGCAGGCG TTGTTGAGGG GGCATTTAGT GGCTGGAGGG TAGTTTTAAA TGTTGACCAG 
TCCAGAGAAG CAGGATTCAA GCGTCTTCTT CAGTCAGGAG GGGCAAAGGT GCTGCCGGGG 
CACTCTCCAT CTCTCTTTAA AGAAGCCACA CACCTCTTCG CGGACTTCAG TAAACTAAAA 
CCAGACGATA CCAGAGTTAA TGTGATGGAA GCTGCTGCCC AAAATGTGAA CTGCTTGAAA 
CCAGAATACA TTGCAGATTA TCTTATGCAG GAGTTGCCTC CCCCGATGGA GAATTACTGC 
CTTCCTGGAG CAGCTGTTCC TCAGAACAGA AAGGAACTCG ATGCAGGTCT ATCCCAGAAG 
AGAAAAGCGC CCGCTGAAAA AAATAAAGTC AAGCGCCTCC GGCTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPBP1 ( XM_007666207.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007664397.1 Ornithorhynchus anatinus topoisomerase (DNA) II binding protein 1 (TOPBP1), mRNA.

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CloneID	OOc06242
Clone ID Related Accession (Same CDS sequence)	XM_007666207.2
Accession Version	XM_007666207.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	LOW QUALITY PROTEIN: DNA topoisomerase 2-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 15, 2016 this sequence version replaced XM_007666207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTCTAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGG AAGAAGTTCA TAAATATGTT 
CAGATGATGG GTGGACGAGT GTACAGAGAT CTTAATGTAT CAGTAACTCA TCTTATTGCT 
GGAGAAGTAG GCAGCAAAAA ATACTTAGTA GCTGCTAGTT TAAAGAAACC TATCATGCGC 
CCTTCTTGGA TAAAAACGCT ATGGGAGAAG TCACAAGAAA ACAGAATGAT CAGATGTACA 
GATGTAGACC TAGAAGAGTT TCGGTGCCCT CTATTTCTTG GCTGCACAAT TTGTGTGACT 
GGATTAAGTA GTCTGGACAG GAAGGAGGTT CAGCGACTAA CGGTAGAACA CGGCGGCCAA 
TACATGGGAC AATTAAAGAT GAATGAAACC ACTCACCTTG TCGTGCAAGA ACCAAAAGGG 
CAGAAATATG AATGTGCCAA GAGATGGAAT GTACATTGTG TGACCATCCA GTGGTTTTTC 
GACAGTATTG AGAGAGGTTT TTGTCAAGAC GAAGTTATGT ATAAGACAGA GCCCAGACCA 
GAAACAAAGA ACCTGCCAGA TACTTCAACT CCTACTGGTC AGACCAGCAA AGCCGATAGT 
CGTACCCTTT CAGACGTCAG CCATATTTCT AATATCAATT CAAGTTGCGT AAATGAGTCT 
GCGTTTACCT CTGCTCTAAA CAGCAAATTG GATTCTCTCC TGGAGGATTT AGAAAATCTG 
GATGTCAGCA CTTTACAAGC ACCAGAAGAT TTACTAGACG GTTGTCGAAT ATATCTCTGT 
GGATTTAGTG GCCGAAAATT AGATAAGCTG AGAAGGCTTA TTAATTGTGG TGGTGGAGTT 
CGTTTTAATC AGCTCAACGA AGATGTCACT CATGTCATTA TAGGAGACTT TGATGATGAA 
TTGAAGCACT TTTGGAATAA GTCAACACAC AGGCCATATG TAGTAGGAGC AAAATGGCTG 
CTGGAGTGCT TTAGTAAGGG CTATCTGCTT TCTGAGGAGC CATATGTTCA CATGAACTAC 
CGAGCAGTGG AAATTTCCGT TTCAGAGCAA CCTGGAATCA AACCTGCCCT TTCAAAAAGA 
AATAGCAATT TGCCTAAGAA AGATTCCGTG GCCTCTGAGG TGCAGCAGCA AGCGGATGAG 
GACCTACTCT CTCAGTATGA GAAAAATGAC TCGACATTAG GGGTGGCTGA GAAATCTGAA 
GCTGGGAAAT TTAATGATTC TACTCATATC CTTTGGAAAG AGGAGGATCA ATCTTCCAGT 
CACAATCACC TTCCTGAGGC TTCTACGATT ATTGAAAGCG GATTGTTCAG CAAGAAAAGG 
TTCCTCGTTG TGGGTTTTTC TGAAGATGAT GAGCCTTGTA TTTCAGATGC TATAAGAGAA 
AATGCTGGGA AAGTTGTATC CCTCCAAAAA AGAATCATTG CTGACTATGC TGTGGTTCCT 
TTACTGGGGT GTAAAGTGGA GTCAACTGTA GGAGAAGTGG TTACAAACAC ATGGCTGGGT 
ATGTGCATTG AGCATCAGGT CCTGTTTGAC CCAGAGTCAA ATCCTCTTTT CACACCACTA 
CCTGTAATGG AAGGGAAAAC CCCTTTAGAA GACTGCGTTC TTTCATTCAG CCAATTTACT 
GGGGCAGAGA GAGACTCCTT GATATACTTA GCAAGTTTGC TTGGAGCGAG AGTTCAAGAG 
TTCTTCGTAC GCAAAGCTAA TACCAAAAAA GGCATGTTTG CCAGTACCCA CCTGGTGCTG 
AAAGAACCTG ATGGTTCCAA ATATGAAGCG GCAAAAAAGT GGAATTTACC TGCAATCACT 
GTGTCTTGGC TCTTACAATC TGCGAGGCTG GGAAAGAAAG CTGAAGAAAG CAAGTTTCTG 
GTCGAGAACG CATCTGCCAA AGAAGAAAGT TCTCAAAGTC AGAAACCAAC AGATCAGACT 
CAAGTATCTG GTTCAGACAC ACCAGAAAAG TCTAGCACAC TCTTGGAGAG CAAAAGGAAC 
TCAGCCGTGA CACCTTTGGA TATGAACAGG TTTCAGAGTA AAGCTTTTCA TACGGTACTC 
TCACAGCAAG CCAGGAAAAC CTCAGTTTCC GGAGAAGGAA AACCACTTCA GAAGGAACCG 
TCGCTACACC TAGACACACC ATCGAAATTT CTGTCCAAAG ACAAGCTCTT CAAGCCTTCA 
TTTGATGTGA AGGATGCCTT GGCAGTTTTG GAAACTCCAA GAGGGTGTAG CCAGAGACAC 
AGGAAGTTGA GCACCCCCCT CTCAGAAGTC ATTGGCAGGA ACCTGAAACT GGCCGTAGCA 
AATAGCACTC GTCACGTAGC GGTTCTTTCC GCAAGTCCTC GGCTGGAGGA TGCCCAGCTG 
GAAAAGTCCA TACAGAAAGA AGAATTCAAA CCATTAACTG ATGTAGTGAT ATGCGTCAGT 
AAGAAACTCA GTAAGAAGCA GAGCGAGCTG AACGGTATTG CAGCATCTCT AGGAGCAGAT 
TACAGGTGGA GCTTCGATGA AACGGTGACA CATTTCATCT ACCAGGGGCG ACAGAACGAC 
AGTAATCGAG AATATAGATC TGTTAAAGAA AGAGGAATAC ATATTGTTTC TGAACACTGG 
CTTTTACAAA GTGCCCAAGA ATACAGACGC CTTCCCGAAT CTCTTTACCC ACACACCTAC 
AATCCCAAAA TGAGCCTGGA TATAAGTGCG ACACAAGATG AGAGGCTCTA TACCGGTCAG 
CTTTCATCAT CTGATAAAAT ACCAAAGGAT AATGAGCCTG AGTCTCTATC TATGGAGGTG 
AAGAATGTAG AAGATGGACC CTCTGATCCG ATGGAGATGG AGACCGCAAA TGACAATCAC 
CCAAATCCCA AAGGAGTGCT AACACAGACC CTGGAAATGA GGGAAAACTT CCAGAAGCAG 
CTGCAAGAAA TCATGTCTGC GACATCAATA GCGAAACCCC AAGGGCAGAG GGGCTCCCTC 
TCCAGAAATG GGTGTGACAG CACTCCTTCA ACTCCAGACA GCGCGCGTTC TGCTCGAAGT 
AGTCGCACCC GAGCCTTGGA AGTGCTAAGA CAGCCTCATC GGACCCTAAC CGATCTCAAC 
ACCGAGCCCT CCCAGAGCGA ACAGATCATT TGGGACGATC CTACGGCACG AGAGGAAAGA 
GCGAGGCTGG CCAGCAACCT GCAGTGGCCC ACCAGTCCCA CGCGACTCTC CGAGCTCCCA 
GCCGACGTGA CAGACTTGGA TGACTCCCCG TTTACGGACT CCTTACATGA CTCTGAAACT 
TCCGAGCTAG CTGCTGCTGA TCCCGAAAAA GCATGGGACA CCGAGGACCC GAAGAACCAG 
ATCTCGGAAG AAGCAGAAAC CACAATGAAA GATGACCACC TGATCCCTAC ACCACAAGCC 
CCCAGCATCG CTTTCCCCCT AGCCAACCCG CCCGTGGCTC CACAACCCAA AGAGAAGACG 
GTACGGGAAA AGGAGACTCG TGAAGAGCTG ACAAAACAAT ACATATTCCA GCTCTCCTCT 
CTCAATCCTC AAGAACGAAT CGACTACTGT CATTTGATCG AGGAACTAGG TGGATCGGTG 
CTTGAAAAGC AGTGCTTTGA CGCGAGCTGC ACGCACATCG TTGTGGGTCA TCCTCTCCGA 
AATGAGAAGT ATTTGGCTTC CATGGCAGCT GGCAAGTGGG TCCTTCATCG CTCCTACCTT 
GAGGCCTGTA GGACCGCCGG GTGCTTTGTG CAGGAGGAAG ATTATGAATG GGGAAGCAGT 
TCCATACTCG ATGTGCTGAC TGGAATCAAC CTGCAGCAGA AAAAATTAGC CCTGGCAGCA 
ATGAGATGGC GGAAGAAAAT CCAGAGAAGA CAAGAAGAAG CAGGCGTTGT TGAGGGGGCA 
TTTAGTGGCT GGAGGGTAGT TTTAAATGTT GACCAGTCCA GAGAAGCAGG ATTCAAGCGT 
CTTCTTCAGT CAGGAGGGGC AAAGGTGCTG CCGGGGCACT CTCCATCTCT CTTTAAAGAA 
GCCACACACC TCTTCGCGGA CTTCAGTAAA CTAAAACCAG ACGATACCAG AGTTAATGTG 
ATGGAAGCTG CTGCCCAAAA TGTGAACTGC TTGAAACCAG AATACATTGC AGATTATCTT 
ATGCAGGAGT TGCCTCCCCC GATGGAGAAT TACTGCCTTC CTGGAGCAGC TGTTCCTCAG 
AACAGAAAGG AACTCGATGC AGGTCTATCC CAGAAGAGAA AAGCGCCCGC TGAAAAAAAT 
AAAGTCAAGC GCCTCCGGCT ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007664397.1
CDS	1..4407
Translation	MSSSDREPFFVKFIKSQDNREFFFTALESIKEFQSEEYLQTITEEEALKIKENDRSLYICDPFGGDVFEHLKKEEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAASLKKPIMRPSWIKTLWEKSQENRMIRCTDVDLEEFRCPLFLGCTICVTGLSSLDRKEVQRLTVEHGGQYMGQLKMNETTHLVVQEPKGQKYECAKRWNVHCVTIQWFFDSIERGFCQDEVMYKTEPRPETKNLPDTSTPTGQTSKADSRTLSDVSHISNINSSCVNESAFTSALNSKLDSLLEDLENLDVSTLQAPEDLLDGCRIYLCGFSGRKLDKLRRLINCGGGVRFNQLNEDVTHVIIGDFDDELKHFWNKSTHRPYVVGAKWLLECFSKGYLLSEEPYVHMNYRAVEISVSEQPGIKPALSKRNSNLPKKDSVASEVQQQADEDLLSQYEKNDSTLGVAEKSEAGKFNDSTHILWKEEDQSSSHNHLPEASTIIESGLFSKKRFLVVGFSEDDEPCISDAIRENAGKVVSLQKRIIADYAVVPLLGCKVESTVGEVVTNTWLGMCIEHQVLFDPESNPLFTPLPVMEGKTPLEDCVLSFSQFTGAERDSLIYLASLLGARVQEFFVRKANTKKGMFASTHLVLKEPDGSKYEAAKKWNLPAITVSWLLQSARLGKKAEESKFLVENASAKEESSQSQKPTDQTQVSGSDTPEKSSTLLESKRNSAVTPLDMNRFQSKAFHTVLSQQARKTSVSGEGKPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAVLETPRGCSQRHRKLSTPLSEVIGRNLKLAVANSTRHVAVLSASPRLEDAQLEKSIQKEEFKPLTDVVICVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRQNDSNREYRSVKERGIHIVSEHWLLQSAQEYRRLPESLYPHTYNPKMSLDISATQDERLYTGQLSSSDKIPKDNEPESLSMEVKNVEDGPSDPMEMETANDNHPNPKGVLTQTLEMRENFQKQLQEIMSATSIAKPQGQRGSLSRNGCDSTPSTPDSARSARSSRTRALEVLRQPHRTLTDLNTEPSQSEQIIWDDPTAREERARLASNLQWPTSPTRLSELPADVTDLDDSPFTDSLHDSETSELAAADPEKAWDTEDPKNQISEEAETTMKDDHLIPTPQAPSIAFPLANPPVAPQPKEKTVREKETREELTKQYIFQLSSLNPQERIDYCHLIEELGGSVLEKQCFDASCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRTAGCFVQEEDYEWGSSSILDVLTGINLQQKKLALAAMRWRKKIQRRQEEAGVVEGAFSGWRVVLNVDQSREAGFKRLLQSGGAKVLPGHSPSLFKEATHLFADFSKLKPDDTRVNVMEAAAQNVNCLKPEYIADYLMQELPPPMENYCLPGAAVPQNRKELDAGLSQKRKAPAEKNKVKRLRLH

Target ORF information:

RefSeq Version	XM_007666207.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus topoisomerase (DNA) II binding protein 1 (TOPBP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007666207.2

ORF Insert Sequence:

ATGTCTAGCA GTGACAGGGA GCCGTTTTTT GTGAAATTTA TAAAGTCTCA GGACAACAGA 
GAATTTTTTT TTACAGCTCT TGAGTCTATA AAAGAATTCC AGTCAGAAGA GTATCTCCAG 
ACTATTACAG AAGAGGAGGC CCTCAAAATA AAAGAAAATG ACAGATCCCT TTATATCTGT 
GACCCATTTG GTGGCGACGT CTTCGAACAC CTCAAAAAGG AAGAAGTTCA TAAATATGTT 
CAGATGATGG GTGGACGAGT GTACAGAGAT CTTAATGTAT CAGTAACTCA TCTTATTGCT 
GGAGAAGTAG GCAGCAAAAA ATACTTAGTA GCTGCTAGTT TAAAGAAACC TATCATGCGC 
CCTTCTTGGA TAAAAACGCT ATGGGAGAAG TCACAAGAAA ACAGAATGAT CAGATGTACA 
GATGTAGACC TAGAAGAGTT TCGGTGCCCT CTATTTCTTG GCTGCACAAT TTGTGTGACT 
GGATTAAGTA GTCTGGACAG GAAGGAGGTT CAGCGACTAA CGGTAGAACA CGGCGGCCAA 
TACATGGGAC AATTAAAGAT GAATGAAACC ACTCACCTTG TCGTGCAAGA ACCAAAAGGG 
CAGAAATATG AATGTGCCAA GAGATGGAAT GTACATTGTG TGACCATCCA GTGGTTTTTC 
GACAGTATTG AGAGAGGTTT TTGTCAAGAC GAAGTTATGT ATAAGACAGA GCCCAGACCA 
GAAACAAAGA ACCTGCCAGA TACTTCAACT CCTACTGGTC AGACCAGCAA AGCCGATAGT 
CGTACCCTTT CAGACGTCAG CCATATTTCT AATATCAATT CAAGTTGCGT AAATGAGTCT 
GCGTTTACCT CTGCTCTAAA CAGCAAATTG GATTCTCTCC TGGAGGATTT AGAAAATCTG 
GATGTCAGCA CTTTACAAGC ACCAGAAGAT TTACTAGACG GTTGTCGAAT ATATCTCTGT 
GGATTTAGTG GCCGAAAATT AGATAAGCTG AGAAGGCTTA TTAATTGTGG TGGTGGAGTT 
CGTTTTAATC AGCTCAACGA AGATGTCACT CATGTCATTA TAGGAGACTT TGATGATGAA 
TTGAAGCACT TTTGGAATAA GTCAACACAC AGGCCATATG TAGTAGGAGC AAAATGGCTG 
CTGGAGTGCT TTAGTAAGGG CTATCTGCTT TCTGAGGAGC CATATGTTCA CATGAACTAC 
CGAGCAGTGG AAATTTCCGT TTCAGAGCAA CCTGGAATCA AACCTGCCCT TTCAAAAAGA 
AATAGCAATT TGCCTAAGAA AGATTCCGTG GCCTCTGAGG TGCAGCAGCA AGCGGATGAG 
GACCTACTCT CTCAGTATGA GAAAAATGAC TCGACATTAG GGGTGGCTGA GAAATCTGAA 
GCTGGGAAAT TTAATGATTC TACTCATATC CTTTGGAAAG AGGAGGATCA ATCTTCCAGT 
CACAATCACC TTCCTGAGGC TTCTACGATT ATTGAAAGCG GATTGTTCAG CAAGAAAAGG 
TTCCTCGTTG TGGGTTTTTC TGAAGATGAT GAGCCTTGTA TTTCAGATGC TATAAGAGAA 
AATGCTGGGA AAGTTGTATC CCTCCAAAAA AGAATCATTG CTGACTATGC TGTGGTTCCT 
TTACTGGGGT GTAAAGTGGA GTCAACTGTA GGAGAAGTGG TTACAAACAC ATGGCTGGGT 
ATGTGCATTG AGCATCAGGT CCTGTTTGAC CCAGAGTCAA ATCCTCTTTT CACACCACTA 
CCTGTAATGG AAGGGAAAAC CCCTTTAGAA GACTGCGTTC TTTCATTCAG CCAATTTACT 
GGGGCAGAGA GAGACTCCTT GATATACTTA GCAAGTTTGC TTGGAGCGAG AGTTCAAGAG 
TTCTTCGTAC GCAAAGCTAA TACCAAAAAA GGCATGTTTG CCAGTACCCA CCTGGTGCTG 
AAAGAACCTG ATGGTTCCAA ATATGAAGCG GCAAAAAAGT GGAATTTACC TGCAATCACT 
GTGTCTTGGC TCTTACAATC TGCGAGGCTG GGAAAGAAAG CTGAAGAAAG CAAGTTTCTG 
GTCGAGAACG CATCTGCCAA AGAAGAAAGT TCTCAAAGTC AGAAACCAAC AGATCAGACT 
CAAGTATCTG GTTCAGACAC ACCAGAAAAG TCTAGCACAC TCTTGGAGAG CAAAAGGAAC 
TCAGCCGTGA CACCTTTGGA TATGAACAGG TTTCAGAGTA AAGCTTTTCA TACGGTACTC 
TCACAGCAAG CCAGGAAAAC CTCAGTTTCC GGAGAAGGAA AACCACTTCA GAAGGAACCG 
TCGCTACACC TAGACACACC ATCGAAATTT CTGTCCAAAG ACAAGCTCTT CAAGCCTTCA 
TTTGATGTGA AGGATGCCTT GGCAGTTTTG GAAACTCCAA GAGGGTGTAG CCAGAGACAC 
AGGAAGTTGA GCACCCCCCT CTCAGAAGTC ATTGGCAGGA ACCTGAAACT GGCCGTAGCA 
AATAGCACTC GTCACGTAGC GGTTCTTTCC GCAAGTCCTC GGCTGGAGGA TGCCCAGCTG 
GAAAAGTCCA TACAGAAAGA AGAATTCAAA CCATTAACTG ATGTAGTGAT ATGCGTCAGT 
AAGAAACTCA GTAAGAAGCA GAGCGAGCTG AACGGTATTG CAGCATCTCT AGGAGCAGAT 
TACAGGTGGA GCTTCGATGA AACGGTGACA CATTTCATCT ACCAGGGGCG ACAGAACGAC 
AGTAATCGAG AATATAGATC TGTTAAAGAA AGAGGAATAC ATATTGTTTC TGAACACTGG 
CTTTTACAAA GTGCCCAAGA ATACAGACGC CTTCCCGAAT CTCTTTACCC ACACACCTAC 
AATCCCAAAA TGAGCCTGGA TATAAGTGCG ACACAAGATG AGAGGCTCTA TACCGGTCAG 
CTTTCATCAT CTGATAAAAT ACCAAAGGAT AATGAGCCTG AGTCTCTATC TATGGAGGTG 
AAGAATGTAG AAGATGGACC CTCTGATCCG ATGGAGATGG AGACCGCAAA TGACAATCAC 
CCAAATCCCA AAGGAGTGCT AACACAGACC CTGGAAATGA GGGAAAACTT CCAGAAGCAG 
CTGCAAGAAA TCATGTCTGC GACATCAATA GCGAAACCCC AAGGGCAGAG GGGCTCCCTC 
TCCAGAAATG GGTGTGACAG CACTCCTTCA ACTCCAGACA GCGCGCGTTC TGCTCGAAGT 
AGTCGCACCC GAGCCTTGGA AGTGCTAAGA CAGCCTCATC GGACCCTAAC CGATCTCAAC 
ACCGAGCCCT CCCAGAGCGA ACAGATCATT TGGGACGATC CTACGGCACG AGAGGAAAGA 
GCGAGGCTGG CCAGCAACCT GCAGTGGCCC ACCAGTCCCA CGCGACTCTC CGAGCTCCCA 
GCCGACGTGA CAGACTTGGA TGACTCCCCG TTTACGGACT CCTTACATGA CTCTGAAACT 
TCCGAGCTAG CTGCTGCTGA TCCCGAAAAA GCATGGGACA CCGAGGACCC GAAGAACCAG 
ATCTCGGAAG AAGCAGAAAC CACAATGAAA GATGACCACC TGATCCCTAC ACCACAAGCC 
CCCAGCATCG CTTTCCCCCT AGCCAACCCG CCCGTGGCTC CACAACCCAA AGAGAAGACG 
GTACGGGAAA AGGAGACTCG TGAAGAGCTG ACAAAACAAT ACATATTCCA GCTCTCCTCT 
CTCAATCCTC AAGAACGAAT CGACTACTGT CATTTGATCG AGGAACTAGG TGGATCGGTG 
CTTGAAAAGC AGTGCTTTGA CGCGAGCTGC ACGCACATCG TTGTGGGTCA TCCTCTCCGA 
AATGAGAAGT ATTTGGCTTC CATGGCAGCT GGCAAGTGGG TCCTTCATCG CTCCTACCTT 
GAGGCCTGTA GGACCGCCGG GTGCTTTGTG CAGGAGGAAG ATTATGAATG GGGAAGCAGT 
TCCATACTCG ATGTGCTGAC TGGAATCAAC CTGCAGCAGA AAAAATTAGC CCTGGCAGCA 
ATGAGATGGC GGAAGAAAAT CCAGAGAAGA CAAGAAGAAG CAGGCGTTGT TGAGGGGGCA 
TTTAGTGGCT GGAGGGTAGT TTTAAATGTT GACCAGTCCA GAGAAGCAGG ATTCAAGCGT 
CTTCTTCAGT CAGGAGGGGC AAAGGTGCTG CCGGGGCACT CTCCATCTCT CTTTAAAGAA 
GCCACACACC TCTTCGCGGA CTTCAGTAAA CTAAAACCAG ACGATACCAG AGTTAATGTG 
ATGGAAGCTG CTGCCCAAAA TGTGAACTGC TTGAAACCAG AATACATTGC AGATTATCTT 
ATGCAGGAGT TGCCTCCCCC GATGGAGAAT TACTGCCTTC CTGGAGCAGC TGTTCCTCAG 
AACAGAAAGG AACTCGATGC AGGTCTATCC CAGAAGAGAA AAGCGCCCGC TGAAAAAAAT 
AAAGTCAAGC GCCTCCGGCT ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TOPBP1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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