GNE cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following GNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22152 NM_005476.7
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu22850 NM_001128227.3
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu22152 XM_017014167.1
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu22444 NM_001190384.3
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu22971 NM_001190383.3
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu23071 NM_001190388.1
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu42653 XM_005251334.4
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu108932 NM_001190388.2
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu108932 NM_001374798.1
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu98397 NM_001374797.1
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu98397 XM_017014168.1
Latest version!
Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu22152 NM_005476.5 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu22152 NM_005476.6 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu22444 NM_001190384.2 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu22971 NM_001190383.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu22850 NM_001128227.2 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu22444 NM_001190384.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu22971 NM_001190383.2 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22152
    Clone ID Related Accession (Same CDS sequence) NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
    Accession Version NM_005476.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, BC121179.2, AL158830.17 and CA503236.1. On Jun 18, 2010 this sequence version replaced NM_005476.4. Transcript Variant: This variant (2) contains a different 5' terminal exon compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG, and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. This variant has been called GNE1 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.39095.1, SRR1803613.148792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005467.1
    CDS222..2390
    Translation

    Target ORF information:

    RefSeq Version NM_005476.5
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005476.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22152
    Clone ID Related Accession (Same CDS sequence) NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
    Accession Version NM_005476.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, BC121179.2, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2018 this sequence version replaced NM_005476.5. Transcript Variant: This variant (2) contains a different 5' terminal exon compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG, and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. This variant has been called GNE1 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.194578.1, SRR1803617.222976.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005467.1
    CDS171..2339
    Translation

    Target ORF information:

    RefSeq Version NM_005476.6
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005476.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22152
    Clone ID Related Accession (Same CDS sequence) NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
    Accession Version NM_005476.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-14
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, BC121179.2, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_005476.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.150925.1, SRR1803617.222976.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005467.1
    CDS171..2339
    Translation

    Target ORF information:

    RefSeq Version NM_005476.7
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005476.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22850
    Clone ID Related Accession (Same CDS sequence) NM_001128227.2 , NM_001128227.3
    Accession Version NM_001128227.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC418354.1, DC416534.1, AK312539.1, BF197948.1, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jun 18, 2010 this sequence version replaced NM_001128227.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). This variant has been called GNE2 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312539.1, AM697708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
    TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG
    CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG
    TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC
    CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC
    ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC
    CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT
    CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA
    ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC
    ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA
    TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT
    CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA
    AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC
    ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC
    CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC
    ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG
    TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA
    GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG
    AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT
    CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG
    ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT
    CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC
    TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA
    ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC
    CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA
    GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC
    AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT
    CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC
    CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA
    ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC
    CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA
    TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC
    TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA
    GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG
    GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA
    GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC
    TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT
    GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121699.1
    CDS113..2374
    Translation

    Target ORF information:

    RefSeq Version NM_001128227.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
    TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG
    CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG
    TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC
    CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC
    ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC
    CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT
    CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA
    ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC
    ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA
    TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT
    CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA
    AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC
    ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC
    CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC
    ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG
    TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA
    GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG
    AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT
    CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG
    ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT
    CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC
    TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA
    ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC
    CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA
    GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC
    AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT
    CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC
    CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA
    ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC
    CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA
    TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC
    TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA
    GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG
    GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA
    GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC
    TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT
    GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22850
    Clone ID Related Accession (Same CDS sequence) NM_001128227.2 , NM_001128227.3
    Accession Version NM_001128227.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC416534.1, AK312539.1, BF197948.1, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001128227.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312539.1, AM697708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
    TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG
    CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG
    TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC
    CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC
    ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC
    CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT
    CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA
    ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC
    ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA
    TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT
    CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA
    AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC
    ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC
    CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC
    ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG
    TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA
    GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG
    AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT
    CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG
    ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT
    CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC
    TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA
    ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC
    CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA
    GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC
    AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT
    CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC
    CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA
    ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC
    CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA
    TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC
    TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA
    GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG
    GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA
    GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC
    TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT
    GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121699.1
    CDS35..2296
    Translation

    Target ORF information:

    RefSeq Version NM_001128227.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128227.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
    TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG
    CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG
    TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC
    CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC
    ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC
    CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT
    CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA
    ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC
    ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA
    TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT
    CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA
    AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC
    ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC
    CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC
    ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG
    TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA
    GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG
    AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT
    CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG
    ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT
    CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC
    TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA
    ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC
    CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA
    GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC
    AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT
    CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC
    CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA
    ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC
    CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA
    TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC
    TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA
    GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG
    GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA
    GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC
    TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT
    GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22152
    Clone ID Related Accession (Same CDS sequence) NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
    Accession Version XM_017014167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869656.1
    CDS67..2235
    Translation

    Target ORF information:

    RefSeq Version XM_017014167.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22444
    Clone ID Related Accession (Same CDS sequence) NM_001190384.3 , NM_001190384.2 , NM_001190384.1
    Accession Version NM_001190384.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, AK295562.1, BC121179.2, AL158830.17 and CA503236.1. On Jun 2, 2019 this sequence version replaced NM_001190384.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295562.1, SRR1803611.217709.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT
    GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG
    GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT
    TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT
    GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG
    TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT
    TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT
    CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG
    GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA
    GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA
    CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
    GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA
    CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
    CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
    GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
    ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
    GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
    GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
    GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
    GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
    CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
    CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
    GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
    AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177313.1
    CDS142..1980
    Translation

    Target ORF information:

    RefSeq Version NM_001190384.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190384.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT
    GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG
    GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT
    TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT
    GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG
    TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT
    TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT
    CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG
    GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA
    GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA
    CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
    GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA
    CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
    CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
    GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
    ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
    GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
    GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
    GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
    GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
    CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
    CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
    GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
    AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22444
    Clone ID Related Accession (Same CDS sequence) NM_001190384.3 , NM_001190384.2 , NM_001190384.1
    Accession Version NM_001190384.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, AK295562.1, BC121179.2, AL158830.17 and CA503236.1. On Jan 20, 2018 this sequence version replaced NM_001190384.1. Transcript Variant: This variant (5) contains a different 5' terminal exon and lacks two alternate 5' coding region segments, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 5) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.217709.1, AK295562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT
    GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG
    GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT
    TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT
    GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG
    TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT
    TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT
    CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG
    GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA
    GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA
    CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
    GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA
    CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
    CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
    GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
    ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
    GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
    GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
    GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
    GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
    CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
    CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
    GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
    AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177313.1
    CDS142..1980
    Translation

    Target ORF information:

    RefSeq Version NM_001190384.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190384.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT
    GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG
    GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT
    TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT
    GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG
    TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT
    TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT
    CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG
    GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA
    GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA
    CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
    GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA
    CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
    CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
    GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
    ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
    GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
    GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
    GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
    GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
    CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
    CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
    GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
    AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22444
    Clone ID Related Accession (Same CDS sequence) NM_001190384.3 , NM_001190384.2 , NM_001190384.1
    Accession Version NM_001190384.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, AK295562.1, BC121179.2, AL158830.17 and CA503236.1. Transcript Variant: This variant (5) contains a different 5' terminal exon and lacks two alternate 5' coding region segments, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 5) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.217709.1, AK295562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT
    GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG
    GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT
    TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT
    GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG
    TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT
    TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT
    CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG
    GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA
    GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA
    CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
    GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA
    CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
    CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
    GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
    ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
    GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
    GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
    GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
    GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
    CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
    CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
    GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
    AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177313.1
    CDS193..2031
    Translation

    Target ORF information:

    RefSeq Version NM_001190384.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT
    GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG
    GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT
    TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT
    GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG
    TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT
    TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT
    CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG
    GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA
    GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA
    CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
    GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA
    CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
    CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
    GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
    ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
    GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
    GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
    GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
    GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
    CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
    CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
    GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
    AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22971
    Clone ID Related Accession (Same CDS sequence) NM_001190383.1 , NM_001190383.2 , NM_001190383.3
    Accession Version NM_001190383.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, EU093084.1, AL158830.17 and CA503236.1. Transcript Variant: This variant (4) contains a different 5' terminal exon and lacks a 3' coding region segment, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 4) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU093084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT
    GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG
    GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG
    GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG
    TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC
    AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG
    AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC
    TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG
    GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG
    GACTACACAA CACGCAGGAT CTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177312.1
    CDS222..2168
    Translation

    Target ORF information:

    RefSeq Version NM_001190383.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT
    GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG
    GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG
    GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG
    TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC
    AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG
    AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC
    TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG
    GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG
    GACTACACAA CACGCAGGAT CTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22971
    Clone ID Related Accession (Same CDS sequence) NM_001190383.1 , NM_001190383.2 , NM_001190383.3
    Accession Version NM_001190383.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, EU093084.1, AL158830.17 and CA503236.1. On Jan 19, 2018 this sequence version replaced NM_001190383.1. Transcript Variant: This variant (4) contains a different 5' terminal exon and lacks a 3' coding region segment, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 4) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU093084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT
    GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG
    GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG
    GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG
    TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC
    AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG
    AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC
    TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG
    GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG
    GACTACACAA CACGCAGGAT CTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177312.1
    CDS171..2117
    Translation

    Target ORF information:

    RefSeq Version NM_001190383.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT
    GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG
    GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG
    GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG
    TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC
    AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG
    AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC
    TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG
    GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG
    GACTACACAA CACGCAGGAT CTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22971
    Clone ID Related Accession (Same CDS sequence) NM_001190383.1 , NM_001190383.2 , NM_001190383.3
    Accession Version NM_001190383.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, EU093084.1, AL158830.17 and CA503236.1. On Jun 2, 2019 this sequence version replaced NM_001190383.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU093084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT
    GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG
    GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG
    GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG
    TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC
    AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG
    AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC
    TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG
    GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG
    GACTACACAA CACGCAGGAT CTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177312.1
    CDS171..2117
    Translation

    Target ORF information:

    RefSeq Version NM_001190383.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190383.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC
    CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT
    ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT
    GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG
    GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG
    GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG
    TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC
    AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG
    AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC
    TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG
    GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG
    GACTACACAA CACGCAGGAT CTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23071
    Clone ID Related Accession (Same CDS sequence) NM_001190388.1
    Accession Version NM_001190388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-23
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC418354.1, AK296687.1, AM697709.1, AL158830.17 and CA503236.1. Transcript Variant: This variant (3) lacks a 5' coding segment, which results in the use of an upstream alternate start codon, compared to variant 1. The predicted protein (isoform 3) has a shorter and distinct N-terminus when it is compared to isoform 1. This variant has been called GNE3 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296687.1, AM697709.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTATTG GTGACTGCTC CGTGGCAGCT AAACCAAGAA AACAGCTGCT CTGCTCATTA 
    TTTCAAACCA CACTAGGGTA CAGAGCTCGT GCTTCGGGAT GGAAACCTAT GGTTATCTGC
    AGAGGGAGTC ATGCTTTCAA GGACCTCATA AATACATATC GAATGATTGA ACAAGATGAC
    TTTGACATTA ACACCAGGCT ACACACAATT GTGAGGGGAG AAGATGAGGC AGCCATGGTG
    GAGTCAGTAG GCCTGGCCCT AGTGAAGCTG CCAGATGTCC TTAATCGCCT GAAGCCTGAT
    ATCATGATTG TTCATGGAGA CAGGTTTGAT GCCCTGGCTC TGGCCACATC TGCTGCCTTG
    ATGAACATCC GAATCCTTCA CATTGAAGGT GGGGAAGTCA GTGGGACCAT TGATGACTCT
    ATCAGACATG CCATAACAAA ACTGGCTCAT TATCATGTGT GCTGCACCCG CAGTGCAGAG
    CAGCACCTGA TATCCATGTG TGAGGACCAT GATCGCATCC TTTTGGCAGG CTGCCCTTCC
    TATGACAAAC TTCTCTCAGC CAAGAACAAA GACTACATGA GCATCATTCG CATGTGGCTA
    GGTGATGATG TAAAATCTAA AGATTACATT GTTGCACTAC AGCACCCTGT GACCACTGAC
    ATTAAGCATT CCATAAAAAT GTTTGAATTA ACATTGGATG CACTTATCTC ATTTAACAAG
    CGGACCCTAG TCCTGTTTCC AAATATTGAC GCAGGGAGCA AAGAGATGGT TCGAGTGATG
    CGGAAGAAGG GCATTGAGCA TCATCCCAAC TTTCGTGCAG TTAAACACGT CCCATTTGAC
    CAGTTTATAC AGTTGGTTGC CCATGCTGGC TGTATGATTG GGAACAGCAG CTGTGGGGTT
    CGAGAAGTTG GAGCTTTTGG AACACCTGTG ATCAACCTGG GAACACGTCA GATTGGAAGA
    GAAACAGGGG AGAATGTTCT TCATGTCCGG GATGCTGACA CCCAAGACAA AATATTGCAA
    GCACTGCACC TTCAGTTTGG TAAACAGTAC CCTTGTTCAA AGATATATGG GGATGGAAAT
    GCTGTTCCAA GGATTTTGAA GTTTCTCAAA TCTATCGATC TTCAAGAGCC ACTGCAAAAG
    AAATTCTGCT TTCCTCCTGT GAAGGAGAAT ATCTCTCAAG ATATTGACCA TATTCTTGAA
    ACTCTAAGTG CCTTGGCCGT TGATCTTGGC GGGACGAACC TCCGAGTTGC AATAGTCAGC
    ATGAAGGGTG AAATAGTTAA GAAGTATACT CAGTTCAATC CTAAAACCTA TGAAGAGAGG
    ATTAATTTAA TCCTACAGAT GTGTGTGGAA GCTGCAGCAG AAGCTGTAAA ACTGAACTGC
    AGAATTTTGG GAGTAGGCAT TTCCACAGGT GGCCGTGTAA ATCCTCGGGA AGGAATTGTG
    CTGCATTCAA CCAAACTGAT CCAAGAGTGG AACTCTGTGG ACCTTAGGAC CCCCCTTTCT
    GACACTTTGC ATCTCCCTGT GTGGGTAGAC AATGATGGCA ACTGTGCTGC CCTGGCGGAA
    AGGAAATTTG GCCAAGGAAA GGGACTGGAA AACTTTGTTA CACTTATCAC AGGCACAGGA
    ATCGGTGGTG GAATTATCCA TCAGCATGAA TTGATCCACG GAAGCTCCTT CTGTGCTGCA
    GAACTGGGCC ACCTTGTTGT GTCTCTGGAT GGGCCTGATT GTTCCTGTGG AAGCCATGGG
    TGCATTGAAG CATACGCCTC TGGAATGGCC TTGCAGAGGG AGGCAAAAAA GCTCCATGAT
    GAGGACCTGC TCTTGGTGGA AGGGATGTCA GTGCCAAAAG ATGAGGCTGT GGGTGCGCTC
    CATCTCATCC AAGCTGCGAA ACTTGGCAAT GCGAAGGCCC AGAGCATCCT AAGAACAGCT
    GGAACAGCTT TGGGTCTTGG GGTTGTGAAC ATCCTCCATA CCATGAATCC CTCCCTTGTG
    ATCCTCTCCG GAGTCCTGGC CAGTCACTAT ATCCACATTG TCAAAGACGT CATTCGCCAG
    CAGGCCTTGT CCTCCGTGCA GGACGTGGAT GTGGTGGTTT CGGATTTGGT TGACCCCGCC
    CTGCTGGGTG CTGCCAGCAT GGTTCTGGAC TACACAACAC GCAGGATCTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177317.1
    CDS15..2168
    Translation

    Target ORF information:

    RefSeq Version NM_001190388.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTATTG GTGACTGCTC CGTGGCAGCT AAACCAAGAA AACAGCTGCT CTGCTCATTA 
    TTTCAAACCA CACTAGGGTA CAGAGCTCGT GCTTCGGGAT GGAAACCTAT GGTTATCTGC
    AGAGGGAGTC ATGCTTTCAA GGACCTCATA AATACATATC GAATGATTGA ACAAGATGAC
    TTTGACATTA ACACCAGGCT ACACACAATT GTGAGGGGAG AAGATGAGGC AGCCATGGTG
    GAGTCAGTAG GCCTGGCCCT AGTGAAGCTG CCAGATGTCC TTAATCGCCT GAAGCCTGAT
    ATCATGATTG TTCATGGAGA CAGGTTTGAT GCCCTGGCTC TGGCCACATC TGCTGCCTTG
    ATGAACATCC GAATCCTTCA CATTGAAGGT GGGGAAGTCA GTGGGACCAT TGATGACTCT
    ATCAGACATG CCATAACAAA ACTGGCTCAT TATCATGTGT GCTGCACCCG CAGTGCAGAG
    CAGCACCTGA TATCCATGTG TGAGGACCAT GATCGCATCC TTTTGGCAGG CTGCCCTTCC
    TATGACAAAC TTCTCTCAGC CAAGAACAAA GACTACATGA GCATCATTCG CATGTGGCTA
    GGTGATGATG TAAAATCTAA AGATTACATT GTTGCACTAC AGCACCCTGT GACCACTGAC
    ATTAAGCATT CCATAAAAAT GTTTGAATTA ACATTGGATG CACTTATCTC ATTTAACAAG
    CGGACCCTAG TCCTGTTTCC AAATATTGAC GCAGGGAGCA AAGAGATGGT TCGAGTGATG
    CGGAAGAAGG GCATTGAGCA TCATCCCAAC TTTCGTGCAG TTAAACACGT CCCATTTGAC
    CAGTTTATAC AGTTGGTTGC CCATGCTGGC TGTATGATTG GGAACAGCAG CTGTGGGGTT
    CGAGAAGTTG GAGCTTTTGG AACACCTGTG ATCAACCTGG GAACACGTCA GATTGGAAGA
    GAAACAGGGG AGAATGTTCT TCATGTCCGG GATGCTGACA CCCAAGACAA AATATTGCAA
    GCACTGCACC TTCAGTTTGG TAAACAGTAC CCTTGTTCAA AGATATATGG GGATGGAAAT
    GCTGTTCCAA GGATTTTGAA GTTTCTCAAA TCTATCGATC TTCAAGAGCC ACTGCAAAAG
    AAATTCTGCT TTCCTCCTGT GAAGGAGAAT ATCTCTCAAG ATATTGACCA TATTCTTGAA
    ACTCTAAGTG CCTTGGCCGT TGATCTTGGC GGGACGAACC TCCGAGTTGC AATAGTCAGC
    ATGAAGGGTG AAATAGTTAA GAAGTATACT CAGTTCAATC CTAAAACCTA TGAAGAGAGG
    ATTAATTTAA TCCTACAGAT GTGTGTGGAA GCTGCAGCAG AAGCTGTAAA ACTGAACTGC
    AGAATTTTGG GAGTAGGCAT TTCCACAGGT GGCCGTGTAA ATCCTCGGGA AGGAATTGTG
    CTGCATTCAA CCAAACTGAT CCAAGAGTGG AACTCTGTGG ACCTTAGGAC CCCCCTTTCT
    GACACTTTGC ATCTCCCTGT GTGGGTAGAC AATGATGGCA ACTGTGCTGC CCTGGCGGAA
    AGGAAATTTG GCCAAGGAAA GGGACTGGAA AACTTTGTTA CACTTATCAC AGGCACAGGA
    ATCGGTGGTG GAATTATCCA TCAGCATGAA TTGATCCACG GAAGCTCCTT CTGTGCTGCA
    GAACTGGGCC ACCTTGTTGT GTCTCTGGAT GGGCCTGATT GTTCCTGTGG AAGCCATGGG
    TGCATTGAAG CATACGCCTC TGGAATGGCC TTGCAGAGGG AGGCAAAAAA GCTCCATGAT
    GAGGACCTGC TCTTGGTGGA AGGGATGTCA GTGCCAAAAG ATGAGGCTGT GGGTGCGCTC
    CATCTCATCC AAGCTGCGAA ACTTGGCAAT GCGAAGGCCC AGAGCATCCT AAGAACAGCT
    GGAACAGCTT TGGGTCTTGG GGTTGTGAAC ATCCTCCATA CCATGAATCC CTCCCTTGTG
    ATCCTCTCCG GAGTCCTGGC CAGTCACTAT ATCCACATTG TCAAAGACGT CATTCGCCAG
    CAGGCCTTGT CCTCCGTGCA GGACGTGGAT GTGGTGGTTT CGGATTTGGT TGACCCCGCC
    CTGCTGGGTG CTGCCAGCAT GGTTCTGGAC TACACAACAC GCAGGATCTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42653
    Clone ID Related Accession (Same CDS sequence) XM_005251334.4
    Accession Version XM_005251334.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
    TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG
    CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG
    TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC
    CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC
    ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC
    CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT
    CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA
    ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC
    ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA
    TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT
    CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251391.1
    CDS113..2221
    Translation

    Target ORF information:

    RefSeq Version XM_005251334.4
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251334.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
    TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG
    CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG
    TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC
    CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC
    ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC
    CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT
    CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA
    ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC
    ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA
    TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT
    CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG GAGCAAAGAG
    ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA
    CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC
    AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA
    CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA
    GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA
    TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA
    GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT
    GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA
    GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA
    ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT
    GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT
    CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT
    AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT
    GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT
    ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC
    TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC
    TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA
    AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG
    GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC
    ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG
    AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA
    GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT
    TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG
    ATCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108932
    Clone ID Related Accession (Same CDS sequence) NM_001190388.2 , NM_001374798.1
    Accession Version NM_001190388.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158830.17. On Oct 16, 2019 this sequence version replaced NM_001190388.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296687.1, AM697709.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG
    CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA
    CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA
    GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT
    AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG
    AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA
    ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC
    CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG
    ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT
    CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT
    ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC
    CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT
    AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA
    GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT
    CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC
    CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC
    TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA
    CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA
    AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT
    TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG
    GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT
    GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG
    AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC
    ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC
    AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG
    GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC
    AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177317.2
    CDS99..2090
    Translation

    Target ORF information:

    RefSeq Version NM_001190388.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190388.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG
    CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA
    CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA
    GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT
    AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG
    AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA
    ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC
    CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG
    ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT
    CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT
    ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC
    CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT
    AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA
    GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT
    CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC
    CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC
    TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA
    CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA
    AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT
    TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG
    GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT
    GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG
    AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC
    ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC
    AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG
    GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC
    AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108932
    Clone ID Related Accession (Same CDS sequence) NM_001190388.2 , NM_001374798.1
    Accession Version NM_001374798.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158830.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.164519.1, SRR1803617.124877.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG
    CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA
    CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA
    GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT
    AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG
    AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA
    ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC
    CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG
    ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT
    CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT
    ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC
    CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT
    AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA
    GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT
    CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC
    CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC
    TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA
    CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA
    AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT
    TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG
    GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT
    GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG
    AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC
    ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC
    AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG
    GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC
    AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361727.1
    CDS142..2133
    Translation

    Target ORF information:

    RefSeq Version NM_001374798.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374798.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
    GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT
    AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG
    GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT
    GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC
    TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT
    TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC
    ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG
    CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA
    CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA
    GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT
    AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG
    AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA
    ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC
    CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG
    ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT
    CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT
    ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC
    CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT
    AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA
    GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT
    CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC
    CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC
    TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA
    CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA
    AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT
    TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG
    GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT
    GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG
    AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC
    ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC
    AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG
    GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC
    AGGATCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98397
    Clone ID Related Accession (Same CDS sequence) NM_001374797.1 , XM_017014168.1
    Accession Version NM_001374797.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158830.17. On Oct 16, 2019 this sequence version replaced XM_017014168.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.111094.1, SRR1803613.74655.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG
    CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT
    GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT
    GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT
    CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT
    GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG
    AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT
    GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC
    GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT
    AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG
    ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC
    ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG
    ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT
    GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA
    AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC
    CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT
    GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC
    TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG
    GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG
    AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT
    GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG
    GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG
    CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC
    ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361726.1
    CDS171..2186
    Translation

    Target ORF information:

    RefSeq Version NM_001374797.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374797.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG
    CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT
    GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT
    GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT
    CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT
    GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG
    AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT
    GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC
    GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT
    AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG
    ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC
    ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG
    ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT
    GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA
    AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC
    CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT
    GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC
    TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG
    GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG
    AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT
    GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG
    GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG
    CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC
    ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98397
    Clone ID Related Accession (Same CDS sequence) NM_001374797.1 , XM_017014168.1
    Accession Version XM_017014168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG
    CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT
    GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT
    GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT
    CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT
    GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG
    AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT
    GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC
    GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT
    AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG
    ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC
    ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG
    ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT
    GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA
    AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC
    CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT
    GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC
    TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG
    GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG
    AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT
    GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG
    GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG
    CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC
    ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869657.1
    CDS199..2214
    Translation

    Target ORF information:

    RefSeq Version XM_017014168.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
    GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA
    CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG
    ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT
    GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT
    CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC
    ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG
    ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC
    ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG
    GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC
    ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG
    CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT
    GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT
    GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT
    CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT
    GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG
    AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT
    GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC
    GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT
    AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG
    ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC
    ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG
    ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT
    GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA
    AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC
    CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT
    GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC
    TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG
    GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG
    AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT
    GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG
    GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG
    CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC
    ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.