Tyw1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Tyw1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tyw1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu12990 NM_178897.4
Latest version!
Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $272.30
$389.00
OMu12980 NM_001015876.2
Latest version!
Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu12980 XM_006504331.3
Latest version!
Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu81205 XM_017320584.2
Latest version!
Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu12980 XM_006504331.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu12980 XM_006504331.2 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu81205 XM_017320584.1 Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu12990
    Clone ID Related Accession (Same CDS sequence) NM_178897.4
    Accession Version NM_178897.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Mus musculus(house mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045279.1 and AC113029.23. On Aug 1, 2009 this sequence version replaced NM_178897.3. Transcript Variant: This variant (2) has multiple differences in its 3' end, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK045279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGGTATTTAT TGGTTTTATT
    CAAATATTAT TTCTATTTTC TGCAACTTAT TTATTTTTGT CCATTTATTT TGTGTGTGCG
    CACATGTGTG GCGGTCAGAG CACAGCCTAC AGGATTTGGT TCTCTTTGTG GGCTCTGGGG
    ACCAAACTGT CTTTTAGTGT GACAGCAGCC ACTTGGATGG CTCCGGTATG GTTCTCAGCA
    GTGCTGTTGT GGTATACTTC ACATGCAGTA CGTGGCTCAC GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_849228.1
    CDS24..1388
    Translation

    Target ORF information:

    RefSeq Version NM_178897.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_178897.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGGTATTTAT TGGTTTTATT
    CAAATATTAT TTCTATTTTC TGCAACTTAT TTATTTTTGT CCATTTATTT TGTGTGTGCG
    CACATGTGTG GCGGTCAGAG CACAGCCTAC AGGATTTGGT TCTCTTTGTG GGCTCTGGGG
    ACCAAACTGT CTTTTAGTGT GACAGCAGCC ACTTGGATGG CTCCGGTATG GTTCTCAGCA
    GTGCTGTTGT GGTATACTTC ACATGCAGTA CGTGGCTCAC GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81205
    Clone ID Related Accession (Same CDS sequence) XM_017320584.1 , XM_017320584.2
    Accession Version XM_017320584.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
    AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG
    CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT
    GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT
    GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG
    GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC
    GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC
    CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC
    CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG
    ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG
    AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC
    TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA
    TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG
    CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA
    CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG
    CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC
    ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG
    ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA
    TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT
    GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG
    GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG
    CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT
    GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT
    GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA
    GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG
    GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG
    AACAAAACGA AGGACATTTC CGGATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176073.1
    CDS624..2213
    Translation

    Target ORF information:

    RefSeq Version XM_017320584.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
    AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG
    CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT
    GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT
    GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG
    GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC
    GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC
    CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC
    CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG
    ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG
    AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC
    TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA
    TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG
    CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA
    CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG
    CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC
    ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG
    ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA
    TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT
    GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG
    GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG
    CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT
    GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT
    GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA
    GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG
    GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG
    AACAAAACGA AGGACATTTC CGGATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu12980
    Clone ID Related Accession (Same CDS sequence) XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
    Accession Version NM_001015876.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Mus musculus(house mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB619468.1, BC068126.1, CD551326.1 and BY670442.1. On Aug 1, 2009 this sequence version replaced NM_001015876.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC068126.1, AK082884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001015876.1
    CDS154..2319
    Translation

    Target ORF information:

    RefSeq Version NM_001015876.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001015876.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu12980
    Clone ID Related Accession (Same CDS sequence) XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
    Accession Version XM_006504331.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504394.1
    Translation

    Target ORF information:

    RefSeq Version XM_006504331.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu12980
    Clone ID Related Accession (Same CDS sequence) XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
    Accession Version XM_006504331.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504394.1
    CDS71..2236
    Translation

    Target ORF information:

    RefSeq Version XM_006504331.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504331.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu12980
    Clone ID Related Accession (Same CDS sequence) XM_006504331.1 , NM_001015876.2 , XM_006504331.2 , XM_006504331.3
    Accession Version XM_006504331.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504394.1
    CDS71..2236
    Translation

    Target ORF information:

    RefSeq Version XM_006504331.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504331.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTCCTC TGGATGTGTG GGACTTGTCG CCTTTGCTGT CACTGTGGAT GAACAGGTTC 
    TACATATATA TGGGCTGTGC CCTGGGCCTT ACCCTTTGCA TCTGTGTCCA GATCATCAAG
    AAGCAGGTCA CTCGTTCCCA GGAGAAACGT GTCCCAGGAG CTCCTGATAG CTCCCTCTCC
    CCTCAGAAGA AGCAGACACA CGTTTCTGGA GTGAAGATTT TTTACGGATC TCAGACGGGC
    ACTGCCAAGG GATTTGCAGT GGTTCTTGCC AAAGCTGTCA CCTCTCTGGA CCTGCCTGTG
    GCCATTATTA ATCTGAAGGA ATATGATCCA GATGACAGTC TGATAGGAGA GATCACTAGT
    AAAACCGTCT GTGCCTTCCT GGTCGCTACG TACACGGACG GCTGCCCTAC AGAGAGTGCA
    GAGTGGTTCT GCAAGTGGTT AGAGGAATCA GCTAATGATT TCCGCTTTGG CAAAACTTAC
    CTGAAGGGAC TTCGCTATGC AGTGTTTGGC CTGGGAGACT CTGCCTACCG GAGCCACTTC
    AACAAGGTCA GCACGAACGT TGACAAATGG CTCTGGATGC TCGGTGCCCA GCGGGTGCTG
    ACCCGAGGGG AAGGCGACTG CAATGCAGTT CAGAGCAAAC ATGGCAGCAT CGAGGCCGAC
    TTCACAGCCT GGAAGACCAA GTTCATCTCC CGGCTGCAGG CCCTGCAGAG AGGGGAGAAA
    AAGGCTTGTG GCGGGAACTG CAAGAGAGGG AAGTGTGAGT CGGCCCAGCA TGGCCCAGGA
    GAGGCGCGGC CTCACCCGCA AGGCGAATTG CATCCTGGGG ATGCTGAGGA GGAAGAGCCC
    TGTGAGAGCA GCAGCGAGGA CGAGCTCGGG ACCCAGGATT ATCAGAGCCT GACTTCTGTG
    GTGGATGTTG AGGACCTGGG CAACATCATG AACCCCGTGA AGAGGGAAAA GAGAGAAAAG
    TCTCATCAGG ACGGGAAAGC TGCCATGCAG AGGAACCCAG AGAAGACTGA GGACGGCGAA
    GGAAGAGCTA TGATAACACC TGCTCTGCGG GAAGCCCTGA CTAAACAAGG GTACCAGCTG
    ATTGGCAGCC ACTCTGGTGT GAAGCTTTGC AGGTGGACAA AGTCGATGCT GCGGGGGAGA
    GGAGGTTGCT ACAAACACAC ATTCTACGGC ATTGAGAGCC ACCGCTGCAT GGAAGCCACC
    CCAAGCTTGG CGTGTGCGAA TAAATGTGTC TTCTGCTGGC GGCACCATAC CAATCCTGTG
    GGCACTGAGT GGCGGTGGAA GATGGACCAG CCAGAATTGA TCTTGAAGGA AGCCATTGAG
    AACCATCAGA ACATGATTAA ACAGTTCAAA GGCGTGCCAG GCCTCAAGGC GGAGCGCTTT
    GAAGAGGGGA TGGAGGTCAA GCACTGTGCG CTGTCACTCG TGGGTGAGCC GATCATGTAC
    CCAGAAATCA ACAGGCTTTT GAAGCTGCTC CACCAGCACG GCATCTCCAG CTTCCTTGTC
    ACCAATGCGC AGTTCCCTGA GGAGATCAGG AAGCTCACAC CAGTTACTCA GCTCTACGTG
    AGCGTGGACG CCAGCACCAG AGACGGCCTG AAGAAGATCG ACCGCCCGCT CTTCAAGGAT
    TTCTGGCAGC GCTTCCTAGA CAGTTTGAAA GCCTTATCTG CAAAGCAACA ACGAACGGTC
    TACAGACTGA CGCTTGTGAA GTGTTGGAAC GTGGATGAAC TCCAGGCCTA CGCTGAGCTG
    GTGTCCTTGG GGAACCCCGA CTTCATCGAG GTCAAGGGTG TGACCTACTG TGGTGAGAGT
    GCAGCCAGCA GCCTCACCAT GGCCAATGTC CCTTGGCACG AGGAAGTGGT TCGGTTTGTC
    CGTGAGCTGG TCGATCTGCT TCCCGACTAT GAAGTTGCGT GTGAACACGA GCACTCCAAC
    TGCCTCCTGA TCGGACACAA GAAGTTTAAG ATCGATGGAG AATGGTGGAC ATGGATCAAT
    TACAGCCGCT TCCAAGAGCT CGTTCTGCAG TACGAAGAGA GTGGAGGCTC CAAGACGTTC
    AGCTCGAGGG ATTACATGGC CAGAACCCCA CAGTGGGCAC TGTTTGGTGC CAGGGAAAGA
    GGCTTTGATC CCAAGGACAC GAGATACCAG AGGAAGAACA AAACGAAGGA CATTTCCGGA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81205
    Clone ID Related Accession (Same CDS sequence) XM_017320584.1 , XM_017320584.2
    Accession Version XM_017320584.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
    AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG
    CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT
    GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT
    GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG
    GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC
    GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC
    CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC
    CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG
    ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG
    AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC
    TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA
    TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG
    CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA
    CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG
    CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC
    ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG
    ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA
    TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT
    GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG
    GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG
    CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT
    GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT
    GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA
    GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG
    GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG
    AACAAAACGA AGGACATTTC CGGATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176073.1
    CDS624..2213
    Translation

    Target ORF information:

    RefSeq Version XM_017320584.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320584.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTCGGTG CCCAGCGGGT GCTGACCCGA GGGGAAGGCG ACTGCAATGC AGTTCAGAGC 
    AAACATGGCA GCATCGAGGC CGACTTCACA GCCTGGAAGA CCAAGTTCAT CTCCCGGCTG
    CAGGCCCTGC AGAGAGGGGA GAAAAAGGCT TGTGGCGGGA ACTGCAAGAG AGGGAAGTGT
    GAGTCGGCCC AGCATGGCCC AGGAGAGGCG CGGCCTCACC CGCAAGGCGA ATTGCATCCT
    GGGGATGCTG AGGAGGAAGA GCCCTGTGAG AGCAGCAGCG AGGACGAGCT CGGGACCCAG
    GATTATCAGA GCCTGACTTC TGTGGTGGAT GTTGAGGACC TGGGCAACAT CATGAACCCC
    GTGAAGAGGG AAAAGAGAGA AAAGTCTCAT CAGGACGGGA AAGCTGCCAT GCAGAGGAAC
    CCAGAGAAGA CTGAGGACGG CGAAGGAAGA GCTATGATAA CACCTGCTCT GCGGGAAGCC
    CTGACTAAAC AAGGGTACCA GCTGATTGGC AGCCACTCTG GTGTGAAGCT TTGCAGGTGG
    ACAAAGTCGA TGCTGCGGGG GAGAGGAGGT TGCTACAAAC ACACATTCTA CGGCATTGAG
    AGCCACCGCT GCATGGAAGC CACCCCAAGC TTGGCGTGTG CGAATAAATG TGTCTTCTGC
    TGGCGGCACC ATACCAATCC TGTGGGCACT GAGTGGCGGT GGAAGATGGA CCAGCCAGAA
    TTGATCTTGA AGGAAGCCAT TGAGAACCAT CAGAACATGA TTAAACAGTT CAAAGGCGTG
    CCAGGCCTCA AGGCGGAGCG CTTTGAAGAG GGGATGGAGG TCAAGCACTG TGCGCTGTCA
    CTCGTGGGTG AGCCGATCAT GTACCCAGAA ATCAACAGGC TTTTGAAGCT GCTCCACCAG
    CACGGCATCT CCAGCTTCCT TGTCACCAAT GCGCAGTTCC CTGAGGAGAT CAGGAAGCTC
    ACACCAGTTA CTCAGCTCTA CGTGAGCGTG GACGCCAGCA CCAGAGACGG CCTGAAGAAG
    ATCGACCGCC CGCTCTTCAA GGATTTCTGG CAGCGCTTCC TAGACAGTTT GAAAGCCTTA
    TCTGCAAAGC AACAACGAAC GGTCTACAGA CTGACGCTTG TGAAGTGTTG GAACGTGGAT
    GAACTCCAGG CCTACGCTGA GCTGGTGTCC TTGGGGAACC CCGACTTCAT CGAGGTCAAG
    GGTGTGACCT ACTGTGGTGA GAGTGCAGCC AGCAGCCTCA CCATGGCCAA TGTCCCTTGG
    CACGAGGAAG TGGTTCGGTT TGTCCGTGAG CTGGTCGATC TGCTTCCCGA CTATGAAGTT
    GCGTGTGAAC ACGAGCACTC CAACTGCCTC CTGATCGGAC ACAAGAAGTT TAAGATCGAT
    GGAGAATGGT GGACATGGAT CAATTACAGC CGCTTCCAAG AGCTCGTTCT GCAGTACGAA
    GAGAGTGGAG GCTCCAAGAC GTTCAGCTCG AGGGATTACA TGGCCAGAAC CCCACAGTGG
    GCACTGTTTG GTGCCAGGGA AAGAGGCTTT GATCCCAAGG ACACGAGATA CCAGAGGAAG
    AACAAAACGA AGGACATTTC CGGATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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