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COL5A1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol COL5A1
Entrez Gene ID 1289
Full Name collagen type V alpha 1 chain
Synonyms EDSC,EDSCL1
General protein information
Preferred Names

collagen type V alpha 1 chain

Names

collagen alpha-1(V) chain
collagen, type V, alpha 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

9

9q34.3
Summary This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013].
Disorder MIM:

120215

Disorder Html: Ehlers-Danlos syndrome, classic type, 130000 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017014266.1 XP_016869755.1 collagen alpha-1(V) chain isoform X1
NM_000093.4 NP_000084.3 collagen alpha-1(V) chain isoform 1 preproprotein
NM_001278074.1 NP_001265003.1 collagen alpha-1(V) chain isoform 2 preproprotein

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Col5a1 NP_604447.1
Homo sapiens (human) COL5A1 NP_001265003.1
Mus musculus (house mouse) Col5a1 NP_056549.2
Gallus gallus (chicken) COL5A1 NP_990121.1
Xenopus tropicalis (tropical clawed frog) col5a1 XP_004916702.1
Danio rerio (zebrafish) col5a1 XP_002665924.2

Gene Ontology


Bibliography

Related articles in PubMed

Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing.
Li H, Wu H, Zhang H, Li Y, Li S, Hou Q, Wu S, Yang SY
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317705334(2017 Jun)

Hypoxia and extra-cellular matrix gene expression in adipose tissue associates with reduced insulin sensitivity in black South African women.
Kotz?-H?rstmann LM, Keswell D, Adams K, Dlamini T, Goedecke JH
Endocrine55(1)144-152(2017 Jan)

Elucidation of the molecular mechanisms of anaplastic thyroid carcinoma by integrated miRNA and mRNA analysis.
Liu G, Wu K, Sheng Y
Oncology reports36(5)3005-3013(2016 Nov)

Characterization of tissue-specific and developmentally regulated alternative splicing of exon 64 in the COL5A1 gene.
Mitchell AL, Judis LM, Schwarze U, Vaynshtok PM, Drumm ML, Byers PH
Connective tissue research53(3)267-76(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both in cell lines and in mouse model, the extracellular matrix receptors including the integrin ( ITGA3 and ITGA2B), collagen ( COL5A1), and laminin ( LAMA5) were significantly inhibited by curcumin at messenger RNA and protein levels.
Title: Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing.

Data suggest that, compared with obese white women, obese black women exhibit higher expression of HIF1A (hypoxia inducible factor 1 alpha), COL5A1 (collagen Valpha1), and COL6A1 (collagen VIalpha1) in gluteal but not abdominal subcutaneous adipose tissue depots; up-regulation of expression of these proteins correlates with reduced insulin sensitivity in black women only.
Title: Hypoxia and extra-cellular matrix gene expression in adipose tissue associates with reduced insulin sensitivity in black South African women.

Variants of the COL5A1 gene seem to influence quadriceps muscle-tendon stiffness.
Title: Human COL5A1 polymorphisms and quadriceps muscle-tendon mechanical stiffness in vivo.

Gene expression levels of three randomly selected DEGs, VCAN, COL5A1 and KCNJ16, were examined using RT-PCR in 10 ATC samples.. angiogenesis was activated by the high expression of CTHRC1, VCAN and POSTN, providing necessary nutrition for tumor cells
Title: Elucidation of the molecular mechanisms of anaplastic thyroid carcinoma by integrated miRNA and mRNA analysis.

COL5A1 mutation is associated with Adrenocortical Adenomas.
Title: Genetic Landscape of Sporadic Unilateral Adrenocortical Adenomas Without PRKACA p.Leu206Arg Mutation.

The following COL5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20536 NM_001278074.1
Latest version!		 Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $951.30
$1359.00
OHu22455 NM_000093.4
Latest version!		 Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu98441 XM_017014266.1
Latest version!		 Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $951.30
$1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu20536
Clone ID Related Accession (Same CDS sequence) NM_001278074.1
Accession Version NM_001278074.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-12-29
Organism Homo sapiens(human)
Product collagen alpha-1(V) chain isoform 2 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591890.31, AB371583.1, CN399221.1, AB209864.1, CN399265.1, CN399198.1, BM051019.1, AI190929.1, CB152831.1, ES314363.1, BC032405.1, CN484933.1 and CA446007.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2 which is of the same length but contains an alternate segment, compared to isoform 1. This variant is based on experimental data in PMID 22149965 and is supported by partial transcripts and RNA-seq data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 22149965 ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001265003.1
CDS384..5900
Misc Feature(1)2
Misc Feature(2)273..275
Misc Feature(3)498..1073
Misc Feature(4)1074..1712
Misc Feature(5)1083..1085
Misc Feature(6)1089..1091
Misc Feature(7)1101..1103
Misc Feature(8)1167..1169
Misc Feature(9)1170..1172
Misc Feature(10)1395..1397
Misc Feature(11)1401..1403
Misc Feature(12)1419..1421
Misc Feature(13)1422..1424
Misc Feature(14)1629..1631
Misc Feature(15)1632..1634
Misc Feature(16)1641..1643
Misc Feature(17)1644..1646
Misc Feature(18)1713..2057
Misc Feature(19)2058..5093
Misc Feature(20)2091..2093
Misc Feature(21)2109..2111
Misc Feature(22)2244..2246
Misc Feature(23)2262..2264
Misc Feature(24)2298..2300
Misc Feature(25)2307..2309
Misc Feature(26)2325..2327
Misc Feature(27)2343..2345
Misc Feature(28)2352..2354
Misc Feature(29)2406..2408
Misc Feature(30)2415..2417
Misc Feature(31)2421..2423
Misc Feature(32)2439..2441
Misc Feature(33)2451..2453
Misc Feature(34)2469..2471
Misc Feature(35)2496..2498
Misc Feature(36)2505..2507
Misc Feature(37)2532..2534
Misc Feature(38)2541..2543
Misc Feature(39)2559..>2684
Misc Feature(40)2559..2561
Misc Feature(41)2577..2579
Misc Feature(42)2613..2615
Misc Feature(43)2631..2633
Misc Feature(44)2649..2651
Misc Feature(45)2667..2669
Misc Feature(46)2676..2678
Misc Feature(47)2694..2696
Misc Feature(48)2703..2705
Misc Feature(49)2721..2723
Misc Feature(50)2748..2750
Misc Feature(51)2766..2768
Misc Feature(52)2793..2795
Misc Feature(53)2802..2804
Misc Feature(54)2811..2813
Misc Feature(55)2829..2831
Misc Feature(56)2838..2840
Misc Feature(57)2883..2885
Misc Feature(58)2919..2921
Misc Feature(59)2964..2966
Misc Feature(60)2973..2975
Misc Feature(61)2991..2993
Misc Feature(62)2994..3173
Misc Feature(63)3000..3002
Misc Feature(64)3009..3011
Misc Feature(65)3027..3029
Misc Feature(66)3045..3047
Misc Feature(67)3054..3056
Misc Feature(68)3072..3074
Misc Feature(69)3090..3092
Misc Feature(70)3099..3101
Misc Feature(71)3171..3173
Misc Feature(72)3216..3218
Misc Feature(73)3432..3434
Misc Feature(74)3441..3443
Misc Feature(75)3450..3452
Misc Feature(76)3468..3470
Misc Feature(77)4044..4046
Misc Feature(78)4053..4055
Misc Feature(79)4596..>4727
Misc Feature(80)4782..4784
Misc Feature(81)4791..4793
Misc Feature(82)4803..4940
Misc Feature(83)5094..5198
Misc Feature(84)5184..5186
Misc Feature(85)5193..5195
Misc Feature(86)5211..5891
Exon (1)1..492
Exon (2)493..660
Exon (3)661..874
Exon (4)875..1037
Exon (5)1038..1169
Exon (6)1170..1307
Exon (7)1308..1547
Exon (8)1548..1715
Exon (9)1716..1772
Exon (10)1773..1814
Exon (11)1815..1877
Exon (12)1878..1952
Exon (13)1953..2045
Exon (14)2046..2102
Exon (15)2103..2156
Exon (16)2157..2210
Exon (17)2211..2264
Exon (18)2265..2318
Exon (19)2319..2372
Exon (20)2373..2417
Exon (21)2418..2471
Exon (22)2472..2516
Exon (23)2517..2570
Exon (24)2571..2615
Exon (25)2616..2669
Exon (26)2670..2714
Exon (27)2715..2768
Exon (28)2769..2813
Exon (29)2814..2867
Exon (30)2868..2975
Exon (31)2976..3029
Exon (32)3030..3083
Exon (33)3084..3128
Exon (34)3129..3182
Exon (35)3183..3227
Exon (36)3228..3281
Exon (37)3282..3335
Exon (38)3336..3389
Exon (39)3390..3497
Exon (40)3498..3587
Exon (41)3588..3641
Exon (42)3642..3749
Exon (43)3750..3857
Exon (44)3858..3911
Exon (45)3912..3965
Exon (46)3966..4073
Exon (47)4074..4127
Exon (48)4128..4235
Exon (49)4236..4289
Exon (50)4290..4397
Exon (51)4398..4451
Exon (52)4452..4505
Exon (53)4506..4559
Exon (54)4560..4613
Exon (55)4614..4721
Exon (56)4722..4775
Exon (57)4776..4829
Exon (58)4830..4937
Exon (59)4938..4991
Exon (60)4992..5027
Exon (61)5028..5081
Exon (62)5082..5337
Exon (63)5338..5450
Exon (64)5451..5519
Exon (65)5520..5753
Exon (66)5754..8440
Translation

Target ORF information:

RefSeq Version NM_001278074.1
Organism Homo sapiens(human)
Definition Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001278074.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22455
Clone ID Related Accession (Same CDS sequence) NM_000093.4
Accession Version NM_000093.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-12-29
Organism Homo sapiens(human)
Product collagen alpha-1(V) chain isoform 1 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591890.31, AB371583.1, CN399221.1, AB209864.1, CN399265.1, BM051019.1, AI190929.1, CB152831.1, ES314363.1, BC032405.1, CN484933.1 and CA446007.1. This sequence is a reference standard in the RefSeqGene project. On May 10, 2013 this sequence version replaced NM_000093.3. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB371583.1, D90279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGGCC AGAATCACTT CTTGGCCCAA AGAAAACCCG 
GGCTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000084.3
CDS384..5900
Misc Feature(1)2
Misc Feature(2)273..275
Misc Feature(3)498..1073
Misc Feature(4)1074..1712
Misc Feature(5)1083..1085
Misc Feature(6)1089..1091
Misc Feature(7)1101..1103
Misc Feature(8)1167..1169
Misc Feature(9)1170..1172
Misc Feature(10)1395..1397
Misc Feature(11)1401..1403
Misc Feature(12)1419..1421
Misc Feature(13)1422..1424
Misc Feature(14)1629..1631
Misc Feature(15)1632..1634
Misc Feature(16)1641..1643
Misc Feature(17)1644..1646
Misc Feature(18)1713..2057
Misc Feature(19)2058..5093
Misc Feature(20)2091..2093
Misc Feature(21)2109..2111
Misc Feature(22)2244..2246
Misc Feature(23)2262..2264
Misc Feature(24)2298..2300
Misc Feature(25)2307..2309
Misc Feature(26)2325..2327
Misc Feature(27)2343..2345
Misc Feature(28)2352..2354
Misc Feature(29)2406..2408
Misc Feature(30)2415..2417
Misc Feature(31)2421..2423
Misc Feature(32)2439..2441
Misc Feature(33)2451..2453
Misc Feature(34)2469..2471
Misc Feature(35)2496..2498
Misc Feature(36)2505..2507
Misc Feature(37)2532..2534
Misc Feature(38)2541..2543
Misc Feature(39)2559..>2684
Misc Feature(40)2559..2561
Misc Feature(41)2577..2579
Misc Feature(42)2613..2615
Misc Feature(43)2631..2633
Misc Feature(44)2649..2651
Misc Feature(45)2667..2669
Misc Feature(46)2676..2678
Misc Feature(47)2694..2696
Misc Feature(48)2703..2705
Misc Feature(49)2721..2723
Misc Feature(50)2748..2750
Misc Feature(51)2766..2768
Misc Feature(52)2793..2795
Misc Feature(53)2802..2804
Misc Feature(54)2811..2813
Misc Feature(55)2829..2831
Misc Feature(56)2838..2840
Misc Feature(57)2883..2885
Misc Feature(58)2919..2921
Misc Feature(59)2964..2966
Misc Feature(60)2973..2975
Misc Feature(61)2991..2993
Misc Feature(62)2994..3173
Misc Feature(63)3000..3002
Misc Feature(64)3009..3011
Misc Feature(65)3027..3029
Misc Feature(66)3045..3047
Misc Feature(67)3054..3056
Misc Feature(68)3072..3074
Misc Feature(69)3090..3092
Misc Feature(70)3099..3101
Misc Feature(71)3171..3173
Misc Feature(72)3216..3218
Misc Feature(73)3432..3434
Misc Feature(74)3441..3443
Misc Feature(75)3450..3452
Misc Feature(76)3468..3470
Misc Feature(77)4044..4046
Misc Feature(78)4053..4055
Misc Feature(79)4782..4784
Misc Feature(80)4791..4793
Misc Feature(81)4803..4940
Misc Feature(82)5094..5198
Misc Feature(83)5184..5186
Misc Feature(84)5193..5195
Misc Feature(85)5211..5891
Exon (1)1..492
Exon (2)493..660
Exon (3)661..874
Exon (4)875..1037
Exon (5)1038..1169
Exon (6)1170..1307
Exon (7)1308..1547
Exon (8)1548..1715
Exon (9)1716..1772
Exon (10)1773..1814
Exon (11)1815..1877
Exon (12)1878..1952
Exon (13)1953..2045
Exon (14)2046..2102
Exon (15)2103..2156
Exon (16)2157..2210
Exon (17)2211..2264
Exon (18)2265..2318
Exon (19)2319..2372
Exon (20)2373..2417
Exon (21)2418..2471
Exon (22)2472..2516
Exon (23)2517..2570
Exon (24)2571..2615
Exon (25)2616..2669
Exon (26)2670..2714
Exon (27)2715..2768
Exon (28)2769..2813
Exon (29)2814..2867
Exon (30)2868..2975
Exon (31)2976..3029
Exon (32)3030..3083
Exon (33)3084..3128
Exon (34)3129..3182
Exon (35)3183..3227
Exon (36)3228..3281
Exon (37)3282..3335
Exon (38)3336..3389
Exon (39)3390..3497
Exon (40)3498..3587
Exon (41)3588..3641
Exon (42)3642..3749
Exon (43)3750..3857
Exon (44)3858..3911
Exon (45)3912..3965
Exon (46)3966..4073
Exon (47)4074..4127
Exon (48)4128..4235
Exon (49)4236..4289
Exon (50)4290..4397
Exon (51)4398..4451
Exon (52)4452..4505
Exon (53)4506..4559
Exon (54)4560..4613
Exon (55)4614..4721
Exon (56)4722..4775
Exon (57)4776..4829
Exon (58)4830..4937
Exon (59)4938..4991
Exon (60)4992..5027
Exon (61)5028..5081
Exon (62)5082..5337
Exon (63)5338..5450
Exon (64)5451..5519
Exon (65)5520..5753
Exon (66)5754..8440
Translation

Target ORF information:

RefSeq Version NM_000093.4
Organism Homo sapiens(human)
Definition Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_000093.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGGCC AGAATCACTT CTTGGCCCAA AGAAAACCCG 
GGCTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98441
Clone ID Related Accession (Same CDS sequence) XM_017014266.1
Accession Version XM_017014266.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product collagen alpha-1(V) chain isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGGTTT GCCCGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869755.1
CDS399..5546
Translation

Target ORF information:

RefSeq Version XM_017014266.1
Organism Homo sapiens(human)
Definition Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017014266.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGGTTT GCCCGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317705334(2017 Jun)
Li H,Wu H,Zhang H,Li Y,Li S,Hou Q,Wu S,Yang SY


book

Hypoxia and extra-cellular matrix gene expression in adipose tissue associates with reduced insulin sensitivity in black South African women.
Endocrine55(1)144-152(2017 Jan)
Kotz?-H?rstmann LM,Keswell D,Adams K,Dlamini T,Goedecke JH


book

Elucidation of the molecular mechanisms of anaplastic thyroid carcinoma by integrated miRNA and mRNA analysis.
Oncology reports36(5)3005-3013(2016 Nov)
Liu G,Wu K,Sheng Y


book

Characterization of tissue-specific and developmentally regulated alternative splicing of exon 64 in the COL5A1 gene.
Connective tissue research53(3)267-76(2012)
Mitchell AL,Judis LM,Schwarze U,Vaynshtok PM,Drumm ML,Byers PH