Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M Nature methods8(6)478-80(2011 Jun)
The following SPATA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPATA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY047940.1 and AY489187.1. On Feb 11, 2015 this sequence version replaced NM_173499.3.
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
##Evidence-Data-START##
Transcript exon combination :: AY489187.1, BC066292.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY047940.1, HY024478.1 and AY489187.1.
Transcript Variant: This variant (4) lacks part of the 5' coding region and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same isoform.
##Evidence-Data-START##
Transcript exon combination :: HY010803.1, HY024478.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY047940.1, HY034703.1 and AY489187.1.
Transcript Variant: This variant (3) lacks part of the 5' coding region and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same isoform.
##Evidence-Data-START##
Transcript exon combination :: HY034703.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2148874 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY047940.1 and AY489187.1.
Transcript Variant: This variant (2) lacks part of the 5' coding region and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same isoform.
##Evidence-Data-START##
Transcript exon combination :: HY047940.1, BI825473.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
On Jun 6, 2016 this sequence version replaced XM_011521265.1.
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##